MD16G1078500.v1.1

DUF1338

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010892
Physical Location & Seq
Forward (+)
5512588 .. 5515351
2764 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1078500.v1.1.491

Sequence Viewer

Length: 1161 bp
ATGAGACTGTCCAGAATGATCTCCTCCGTTACCTCCTCCTCATCCGCCATTAACAGAACCTCATCAACCCTTTACTCTCTCACTCCTCTCCAACCAAACGTTAATCCCATCACCATCACCCGGAGGAATTTCATCTCCCTCACCGAATCATCATCCTTTCCCCTCAGACATTCAATCTCCATGAACAAGCAATATGTCTCTACGCTTTCCAGCTCCAGATCAGACAACGGAACATCCCTTGATCCTCCTTCCCAGGGAGCCGAATCATTTTTCAGGGCAGTGTTGGCAAGCATGGAGGCTGTCTACTTAAGCAGAAACCCAACTGCAAAAGCAATCTTGGAGCTTGTTGGGTCGGCTTCCGATGAACAAATTTACTATGACCATTTCGCATTCAGGACTTTTGGGGTGAATGGTTATGGCATTGATTCCATGGCCAAAATTTTCCTGGATTTTGGGTACACGCCCCGGGAGGAATTGAGATTCCCAGCTAAAAAATTGAGAGCATTGTGGTTTTCTCCCCCCAGTGTTCCCATTCCTCATGGCGGCAGTGGTGTGAATGGGCCTCTGCCGAGAGTTTTCATATCCGAGCTTCTTGTAGATCAGATGAGTCCCCAAACTCAGGATATAATAAAAAAGTACACTGAAATATCTGGTTCTGGAAATAAACATGCTGCTCTAGCAAGTGCTCTCGGATCTTTAACGTGGGAGAAGCCGTTACATTCTGAATTCCAACAATTGGCTAGGGAAAGCGAATATGCTGCCTGGACGCTTGTCAATGGGTATGCACTGAACCATGCCACCATCTCCACTCATCAGCTGAAATCGCACTTGAGAGATATTAAAAGCCTCAACCAGTTCATTGAGAAGAATGGTTTCAAGTTGAATTCTGAAGGGGGAATTCTTAAAGTGAGCCCCGATGGCCTTCTTCAGCAAAGTTCAACAGTCGCAGATTCAATCCATTTCCAATTTGCGGATGGTGTAACTGAATCAGTCCCCTGCTCCTACATCGAGTTTGCTGAACGCCTGGTTCTGCCTCAATACAGCAATCTGCCCCTTGACGAGGTTAAAGAGCATCACAGGCGAGACGGATTTGAGGTTGGAAATGCCGACAAGATCTTCGAGAGCACATCAAAGGAGCAGCTGACCAGGAAGGCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1338

Protein Analysis

387

Amino Acids

42.72

Weight (kDa)

7.2

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HGLS PF07063 95 - 344 1.7e-42 2-oxoadipate dioxygenase/decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 736
AccI GTMKAC 1 cut(s) 303
AciI CCGC 3 cut(s) 45, 543, 971
AclI AACGTT 1 cut(s) 99
AclWI GGATC 2 cut(s) 236, 700
AcoI YGGCCR 1 cut(s) 432
AcsI RAATTY 6 cut(s) 127, 369, 438, 725, 883, 897
AcuI CTGAAG 2 cut(s) 909, 911
AfaI GTAC 2 cut(s) 458, 638
AfiI CCNNNNNNNGG 7 cut(s) 120, 254, 542, 619, 736, 970, 1060
AflII CTTAAG 1 cut(s) 307
AgsI TTSAA 5 cut(s) 174, 877, 883, 939, 954
AjnI CCWGG 5 cut(s) 252, 444, 761, 1023, 1145
AluBI AGCT 6 cut(s) 213, 343, 488, 589, 817, 1141
AluI AGCT 6 cut(s) 213, 343, 488, 589, 817, 1141
Alw21I GWGCWC 2 cut(s) 688, 1127
Alw26I GTCTC 2 cut(s) 202, 1077
AlwI GGATC 2 cut(s) 236, 700
Ama87I CYCGRG 1 cut(s) 465
AoxI GGCC 3 cut(s) 432, 560, 919
ApeKI GCWGC 4 cut(s) 671, 758, 1138, 1154
ApoI RAATTY 6 cut(s) 127, 369, 438, 725, 883, 897
ArsI GACNNNNNNTTYG 2 cut(s) 1100, 1132
Asp700I GAANNNNTTC 1 cut(s) 872
AspS9I GGNCC 1 cut(s) 560
AsuC2I CCSGG 3 cut(s) 121, 466, 467
AsuHPI GGTGA 4 cut(s) 103, 109, 133, 418
AvaI CYCGRG 1 cut(s) 465
BalI TGGCCA 1 cut(s) 434
BanII GRGCYC 1 cut(s) 914
Bbv12I GWGCWC 2 cut(s) 688, 1127
BbvI GCAGC 4 cut(s) 658, 745, 1141, 1150
BccI CCATC 5 cut(s) 116, 122, 809, 911, 968
BceAI ACGGC 1 cut(s) 697
BcgI CGANNNNNNTGC 2 cut(s) 740, 774
BciT130I CCWGG 5 cut(s) 254, 446, 763, 1025, 1147
BcnI CCSGG 3 cut(s) 121, 466, 467
BcoDI GTCTC 2 cut(s) 202, 1077
BfaI CTAG 2 cut(s) 677, 741
BfrI CTTAAG 1 cut(s) 307
BglI GCCNNNNNGGC 1 cut(s) 918
BglII AGATCT 1 cut(s) 1113
BisI GCNGC 5 cut(s) 544, 672, 759, 1139, 1155
BlsI GCNGC 5 cut(s) 545, 673, 760, 1140, 1156
Bme1390I CCNGG 8 cut(s) 121, 254, 446, 466, 467, 763, 1025, 1147
BmeT110I CYCGRG 1 cut(s) 465
BmgT120I GGNCC 1 cut(s) 560
BmiI GGNNCC 1 cut(s) 259
BmrFI CCNGG 8 cut(s) 121, 254, 446, 466, 467, 763, 1025, 1147
BmrI ACTGGG 1 cut(s) 516
BmsI GCATC 1 cut(s) 1081
BmuI ACTGGG 1 cut(s) 516
BoxI GACNNNNGTC 1 cut(s) 770
BpmI CTGGAG 1 cut(s) 199
BpuEI CTTGAG 1 cut(s) 850
BpuMI CCSGG 3 cut(s) 121, 466, 467
BsaJI CCNNGG 5 cut(s) 252, 253, 429, 464, 465
BsaXI ACNNNNNCTCC 2 cut(s) 698, 728
Bsc4I CCNNNNNNNGG 7 cut(s) 120, 254, 542, 619, 736, 970, 1060
Bse1I ACTGG 2 cut(s) 522, 853
BseBI CCWGG 5 cut(s) 254, 446, 763, 1025, 1147
BseDI CCNNGG 5 cut(s) 252, 253, 429, 464, 465
BseGI GGATG 4 cut(s) 41, 152, 233, 979
BseLI CCNNNNNNNGG 7 cut(s) 120, 254, 542, 619, 736, 970, 1060
BseMII CTCAG 2 cut(s) 178, 632
BseNI ACTGG 2 cut(s) 522, 853
BseRI GAGGAG 4 cut(s) 13, 25, 28, 75
BseXI GCAGC 4 cut(s) 658, 745, 1141, 1150
BseYI CCCAGC 1 cut(s) 484
BshFI GGCC 3 cut(s) 434, 562, 921
BsiHKAI GWGCWC 2 cut(s) 688, 1127
BsiHKCI CYCGRG 1 cut(s) 465
BsiSI CCGG 2 cut(s) 121, 466
BslFI GGGAC 2 cut(s) 594, 977
BslI CCNNNNNNNGG 7 cut(s) 120, 254, 542, 619, 736, 970, 1060
BsmAI GTCTC 2 cut(s) 202, 1077
BsmBI CGTCTC 1 cut(s) 1077
BsmFI GGGAC 2 cut(s) 594, 977
BsmI GAATGC 1 cut(s) 389
BsnI GGCC 3 cut(s) 434, 562, 921
BsoBI CYCGRG 1 cut(s) 465
Bsp1286I GDGCHC 3 cut(s) 688, 914, 1127
Bsp143I GATC 6 cut(s) 18, 218, 241, 598, 692, 1113
Bsp19I CCATGG 1 cut(s) 429
BspACI CCGC 3 cut(s) 45, 543, 971
BspANI GGCC 3 cut(s) 434, 562, 921
BspCNI CTCAG 2 cut(s) 177, 631
BspLI GGNNCC 1 cut(s) 259
BspPI GGATC 2 cut(s) 236, 700
BspTI CTTAAG 1 cut(s) 307
BsrI ACTGG 2 cut(s) 522, 853
BssECI CCNNGG 5 cut(s) 252, 253, 429, 464, 465
BssMI GATC 6 cut(s) 18, 218, 241, 598, 692, 1113
BssT1I CCWWGG 1 cut(s) 429
Bst2UI CCWGG 5 cut(s) 254, 446, 763, 1025, 1147
Bst4CI ACNGT 2 cut(s) 9, 943
BstAFI CTTAAG 1 cut(s) 307
BstC8I GCNNGC 1 cut(s) 289
BstDEI CTNAG 2 cut(s) 164, 618
BstDSI CCRYGG 1 cut(s) 429
BstENI CCTNNNNNAGG 1 cut(s) 1058
BstF5I GGATG 4 cut(s) 41, 152, 233, 979
BstKTI GATC 6 cut(s) 21, 221, 244, 601, 695, 1116
BstMAI GTCTC 2 cut(s) 202, 1077
BstMBI GATC 6 cut(s) 18, 218, 241, 598, 692, 1113
BstMWI GCNNNNNNNGC 5 cut(s) 284, 677, 823, 918, 1078
BstNI CCWGG 5 cut(s) 254, 446, 763, 1025, 1147
BstNSI RCATGY 1 cut(s) 671
BstPAI GACNNNNGTC 1 cut(s) 770
BstSCI CCNGG 8 cut(s) 119, 252, 444, 464, 465, 761, 1023, 1145
BstV1I GCAGC 4 cut(s) 658, 745, 1141, 1150
BstX2I RGATCY 2 cut(s) 692, 1113
BstYI RGATCY 2 cut(s) 692, 1113
BsuRI GGCC 3 cut(s) 434, 562, 921
BtgI CCRYGG 1 cut(s) 429
BtsCI GGATG 4 cut(s) 41, 152, 233, 979
BtsI GCAGTG 2 cut(s) 285, 553
BtsIMutI CAGTG 5 cut(s) 285, 529, 553, 639, 785
Cac8I GCNNGC 1 cut(s) 289
Cfr13I GGNCC 1 cut(s) 560
Cfr9I CCCGGG 1 cut(s) 465
CseI GACGC 1 cut(s) 775
Csp6I GTAC 2 cut(s) 457, 637
CviAII CATG 7 cut(s) 181, 292, 430, 539, 668, 794, 1158
CviQI GTAC 2 cut(s) 457, 637
DdeI CTNAG 2 cut(s) 164, 618
DpnI GATC 6 cut(s) 20, 220, 243, 600, 694, 1115
DpnII GATC 6 cut(s) 18, 218, 241, 598, 692, 1113
EaeI YGGCCR 1 cut(s) 432
EciI GGCGGA 1 cut(s) 34
Eco130I CCWWGG 1 cut(s) 429
Eco24I GRGCYC 1 cut(s) 914
Eco57I CTGAAG 2 cut(s) 909, 911
Eco88I CYCGRG 1 cut(s) 465
EcoNI CCTNNNNNAGG 1 cut(s) 1058
EcoRI GAATTC 3 cut(s) 725, 883, 897
EcoRII CCWGG 5 cut(s) 252, 444, 761, 1023, 1145
EcoT14I CCWWGG 1 cut(s) 429
EcoT38I GRGCYC 1 cut(s) 914
ErhI CCWWGG 1 cut(s) 429
Esp3I CGTCTC 1 cut(s) 1077
FaeI CATG 7 cut(s) 184, 295, 433, 542, 671, 797, 1161
FaqI GGGAC 2 cut(s) 594, 977
FatI CATG 7 cut(s) 180, 291, 429, 538, 667, 793, 1157
FblI GTMKAC 1 cut(s) 303
Fnu4HI GCNGC 5 cut(s) 544, 672, 759, 1139, 1155
FokI GGATG 4 cut(s) 28, 139, 220, 986
FriOI GRGCYC 1 cut(s) 914
Fsp4HI GCNGC 5 cut(s) 544, 672, 759, 1139, 1155
FspBI CTAG 2 cut(s) 677, 741
GluI GCNGC 5 cut(s) 544, 672, 759, 1139, 1155
GsaI CCCAGC 1 cut(s) 488
GsuI CTGGAG 1 cut(s) 199
HaeIII GGCC 3 cut(s) 434, 562, 921
HapII CCGG 2 cut(s) 121, 466
HgaI GACGC 1 cut(s) 775
Hin1II CATG 7 cut(s) 184, 295, 433, 542, 671, 797, 1161
HinfI GANTC 7 cut(s) 146, 263, 425, 480, 607, 950, 986
HpaII CCGG 2 cut(s) 121, 466
HphI GGTGA 4 cut(s) 103, 109, 133, 418
Hpy166II GTNNAC 3 cut(s) 304, 459, 639
Hpy188I TCNGA 8 cut(s) 167, 223, 361, 586, 603, 692, 724, 889
Hpy188III TCNNGA 6 cut(s) 12, 216, 394, 620, 657, 1120
Hpy8I GTNNAC 3 cut(s) 304, 459, 639
HpyAV CCTTC 4 cut(s) 258, 884, 932, 1144
HpyCH4III ACNGT 2 cut(s) 9, 943
HpyCH4IV ACGT 2 cut(s) 99, 701
HpyCH4V TGCA 3 cut(s) 326, 785, 1157
HpyF10VI GCNNNNNNNGC 5 cut(s) 284, 677, 823, 918, 1078
HpyF3I CTNAG 2 cut(s) 164, 618
HpySE526I ACGT 2 cut(s) 99, 701
Hsp92II CATG 7 cut(s) 184, 295, 433, 542, 671, 797, 1161
Kzo9I GATC 6 cut(s) 18, 218, 241, 598, 692, 1113
LmnI GCTCC 5 cut(s) 218, 257, 340, 1004, 1135
Lsp1109I GCAGC 4 cut(s) 658, 745, 1141, 1150
LweI GCATC 1 cut(s) 1081
MaeI CTAG 2 cut(s) 677, 741
MaeII ACGT 2 cut(s) 99, 701
MaeIII GTNAC 3 cut(s) 28, 714, 979
MalI GATC 6 cut(s) 20, 220, 243, 600, 694, 1115
MboI GATC 6 cut(s) 18, 218, 241, 598, 692, 1113
MboII GAAGA 3 cut(s) 877, 917, 1108
MfeI CAATTG 1 cut(s) 734
MflI RGATCY 2 cut(s) 692, 1113
MhlI GDGCHC 3 cut(s) 688, 914, 1127
MlsI TGGCCA 1 cut(s) 434
MluNI TGGCCA 1 cut(s) 434
MlyI GAGTC 1 cut(s) 616
MmeI TCCRAC 3 cut(s) 115, 754, 1078
Mox20I TGGCCA 1 cut(s) 434
MroXI GAANNNNTTC 1 cut(s) 872
MscI TGGCCA 1 cut(s) 434
MseI TTAA 7 cut(s) 51, 102, 308, 698, 840, 903, 1065
Msp20I TGGCCA 1 cut(s) 434
MspA1I CMGCKG 2 cut(s) 817, 1141
MspCI CTTAAG 1 cut(s) 307
MspI CCGG 2 cut(s) 121, 466
MspR9I CCNGG 8 cut(s) 121, 254, 446, 466, 467, 763, 1025, 1147
MunI CAATTG 1 cut(s) 734
Mva1269I GAATGC 1 cut(s) 389
MvaI CCWGG 5 cut(s) 254, 446, 763, 1025, 1147
MwoI GCNNNNNNNGC 5 cut(s) 284, 677, 823, 918, 1078
NciI CCSGG 3 cut(s) 121, 466, 467
NcoI CCATGG 1 cut(s) 429
NdeII GATC 6 cut(s) 18, 218, 241, 598, 692, 1113
NlaIII CATG 7 cut(s) 184, 295, 433, 542, 671, 797, 1161
NlaIV GGNNCC 1 cut(s) 259
NmeAIII GCCGAG 1 cut(s) 594
NspI RCATGY 1 cut(s) 671
PasI CCCWGGG 1 cut(s) 253
PctI GAATGC 1 cut(s) 389
PdmI GAANNNNTTC 1 cut(s) 872
PfeI GAWTC 6 cut(s) 146, 263, 425, 480, 950, 986
PflMI CCANNNNNTGG 1 cut(s) 736
PfoI TCCNGGA 1 cut(s) 444
PkrI GCNGC 5 cut(s) 545, 673, 760, 1140, 1156
PleI GAGTC 1 cut(s) 615
PpsI GAGTC 1 cut(s) 615
PshAI GACNNNNGTC 1 cut(s) 770
Psp1406I AACGTT 1 cut(s) 99
Psp6I CCWGG 5 cut(s) 252, 444, 761, 1023, 1145
PspFI CCCAGC 1 cut(s) 484
PspGI CCWGG 5 cut(s) 252, 444, 761, 1023, 1145
PspN4I GGNNCC 1 cut(s) 259
PspPI GGNCC 1 cut(s) 560
PsuI RGATCY 2 cut(s) 692, 1113
PvuII CAGCTG 2 cut(s) 817, 1141
RsaI GTAC 2 cut(s) 458, 638
RsaNI GTAC 2 cut(s) 457, 637
SaqAI TTAA 7 cut(s) 51, 102, 308, 698, 840, 903, 1065
SatI GCNGC 5 cut(s) 544, 672, 759, 1139, 1155
Sau3AI GATC 6 cut(s) 18, 218, 241, 598, 692, 1113
Sau96I GGNCC 1 cut(s) 560
SchI GAGTC 1 cut(s) 616
ScrFI CCNGG 8 cut(s) 121, 254, 446, 466, 467, 763, 1025, 1147
SduI GDGCHC 3 cut(s) 688, 914, 1127
SfaNI GCATC 1 cut(s) 1081
SmaI CCCGGG 1 cut(s) 467
SmlI CTYRAG 2 cut(s) 307, 829
SmoI CTYRAG 2 cut(s) 307, 829
SsiI CCGC 3 cut(s) 45, 543, 971
SspMI CTAG 2 cut(s) 677, 741
StyD4I CCNGG 8 cut(s) 119, 252, 444, 464, 465, 761, 1023, 1145
StyI CCWWGG 1 cut(s) 429
TaaI ACNGT 2 cut(s) 9, 943
TaiI ACGT 2 cut(s) 102, 704
TaqI TCGA 2 cut(s) 1008, 1119
TatI WGTACW 1 cut(s) 636
TauI GCSGC 1 cut(s) 546
TfiI GAWTC 6 cut(s) 146, 263, 425, 480, 950, 986
Tru1I TTAA 7 cut(s) 51, 102, 308, 698, 840, 903, 1065
Tru9I TTAA 7 cut(s) 51, 102, 308, 698, 840, 903, 1065
TscAI CASTG 5 cut(s) 285, 529, 553, 646, 792
TseI GCWGC 4 cut(s) 671, 758, 1138, 1154
TspDTI ATGAA 5 cut(s) 121, 197, 378, 568, 847
TspGWI ACGGA 3 cut(s) 16, 243, 1101
TspMI CCCGGG 1 cut(s) 465
TspRI CASTG 5 cut(s) 285, 529, 553, 646, 792
Van91I CCANNNNNTGG 1 cut(s) 736
Vha464I CTTAAG 1 cut(s) 307
XagI CCTNNNNNAGG 1 cut(s) 1058
XapI RAATTY 6 cut(s) 127, 369, 438, 725, 883, 897
XceI RCATGY 1 cut(s) 671
XcmI CCANNNNNNNNNTGG 2 cut(s) 442, 971
XmaI CCCGGG 1 cut(s) 465
XmiI GTMKAC 1 cut(s) 303
XmnI GAANNNNTTC 1 cut(s) 872
XspI CTAG 2 cut(s) 677, 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.