FvH4_4g32470

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005436
Physical Location & Seq
Reverse (-)
31101201 .. 31110649
9449 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g32470.t1

Sequence Viewer

Length: 2970 bp
ATGGCACTGGCAGTTTCAGCTAGCATGTGTTTCGGAGGAAATGGATTGAGGAGCGCAATAGCTTTTGAAGCTGAGAAGGGATATCGGAATGCATTGGGTAGCAAAATGAGGTTCAGTACTTTTCTCCTTTCTAAAATATTGAAAATTTGTTCTCGATCAAAGCATACTTTTGCTAAGAGTATTTTGGAGGAGGCAGATAGTTATGGCATAGCAAGCGTCTCAGATCGATCAAAGTTACTTAACAAGGTATCTGTTTTGATGGGTTATGATGGTCTTCATGATTTGATTGAGAATGAAAGAGCTGAAAAGCATTATGGTATGAATATTAAAGATGCAATGGATGATTTTGATGTCTCGTTCGTCTGCCAGAGATTTCCATCAATCATTTTGGGTAGTTCCCCTCAAGTGGAATTGTATGATGGGACTGCTAATTTCTTTGAAAAGTTGACACCTTTAACGACTCAAGGTCCAGAAGGATTTTCTTCTGATTCTGCGGTTGAAGAACAGGAGGGAGATCATCTCTATGAAACAGGGGATTCTCTGTATCCTTCTTTTCCAAGTGCTGAAACTTCCATATTAACAGAGGATCAGTCAAAGACTTTGGCTGCTGAGAGACACTCCTATCAACCAGTTCCCGTGGATGAATCTTCTAACAAAGTGTCAGTTAAATCACAAAAGAACATTGTGCCTGATGAGTTGTTCCTGGACAATCCTATCAGTTGTGTACCTGGAATAAGTAAGAAGCGTATTAATCAGCTAGAAAATTGTGGCTTTCATACGGTTCGCAAATTGCTGCACCACTTTCCTCGGACCTATGCTGATTTACAGAATGCTCAAATTGAAATTGATGATGGACAATACTTGATTTTCATTGGAAAGATATTAAAATCAAGGGGAATCAAAGCCGGTGCTTCTTTTTCCATTGTTGAGGTTGTCGTGGGATGTGAGATTGCTGATGATAAAATAATGGACAATCAAGATGATTCTACCGATTGTAGAAGGAAAACAATATATGTGCATCTGAAGAAATTTTTTCGTGGTACACGTTTCACTTCTCTACCGTTTCTTAGAATTGTTGAGCAGAAGCACAAAGAGGGAGACTTTGTTTGTGTTAGTGGTAAGGTGAGGACAATGCCTACCAAAGATCACTATGAAATGAGAGAATATAATATTGATGTCCTTAAGGATGAAAATGACTTGTCTTTTCATGCAAAAGGGAGGCCATATCCTATATACCCTTCAAAAGGAGGCTTAAATCCAAACATTTTGAGAGATATCATTGAAAGAGTTGTACAAGTTTTACCTGTCAATATTGATCCTATTCCTAAAAGTATCATTCAGGAATTTGGGCTGCTCAGTCTTCATGATGCGTACACTGAGATTCACCAACCGAAGAGTATGAGTGAAGCTGATTTGGCTCGGAAAAGGCTAATATTTGACGAGTTCTTTTACCTTCAGCTAGCGCGCTTATACCAAATGCTTGAAGGGCTTGGTACACAGATAGAGAAAGATGGATTGCTAGATAAGTACAGGAAACCTGAATCAAGTGCTGCTTATATGGAAGATTGGTCCAATCTTACCAAGAAGTTCCTTAAAGCTCTTCCATATGCTCTTACAGCCAGTCAGCTCACTGCTGTTTCAGAAATTATATGGGACCTCAGGCAACCAGTGCCCATGAATCGGCTATTGCAGGGTGATGTTGGCTGTGGTAAAACTGTAGTTGCTTTTCTAGCATGCATGGAGGTAATAGGCTCTGGATATCAGGCAGCTTTCATGGTTCCTACTGAGTTGCTTGCTGTCCAGCATTATGAGCACTTAAAGAATTTGCTTGAAACTATGGAGGATGTTGAGTTCAAACCTACTATTGCGTTACTAACAGGATCAACCCCGTCAAAACAATCACGGATGATTCAAAAGAGTCTCCAGACTGGAGAGATATCAATGGTCATTGGAACAACCAGTTTGATAGCTGACCGTGTGGAGTTCTCTGCATTACGGATTGCTGTGGTGGATGAACAACATCGTTTCGGTGTCATCCAGAGAGGACGGTTTAACAGTAAGCTATACTATACCTCCATAAGTTCTAACATGCTAGCCACAAACTCAGATGGGACCTCCAAATGTGAAAAGCATATGGCTCCTCATGTTCTTGCCATGTCAGCTACTCCGATCCCAAGGACACTTGCTCTGGCCTTATACGGGGATATGTCATTAACACAAATAACAGACTTGCCACCTGGAAGAATACCGGTGGAGACATTCATTATTCAAGGAAATGAAAATGGGTATGAGGATGCTTATGAGATGATGCTAGATGAGTTAAAAGAAGGAGGCAAAGTATATCTAGTGTATCCGGTCATTGAACAATCTGAGCAGCTGCCTCAACTTCGGGCAGCTTCTGCGGATTTTGAAGCTATATCTCATAGGTTTCGCGGCTACAGCTGCGGATTGTTGCATGGAAAAATGAAGAGTGATGAGAAAGATGAAGCATTGAGAAAGTTTAGGTCTGGTGAAACAGATATACTTCTTGCCACGCAAGTGATTGAGATTGGTGTTGATGTCCCAGATGCATCTATGATGGTTGTTATGAACGCTGAGAGGTTTGGGATTGCTCAGTTGCATCAACTTAGAGGACGAGTTGGACGAGGAGTGAGAAAGTCTAAGTGTTTACTGTTAGCATCTTCTGAGAGCAGCCTGCCACGGCTGAGGGTCCTCGGAAAATCATCAGATGGGTTTTACTTGGCTAATATGGACCTTCTTTTACGTGGACCTGGTAACTTGCTTGGCAAGAAACAGTCGGGACATCTTCCAGAGTTTCCTATTGCAAGATTGGAAATGGATGGCAATATCTTACAAGAAGCACACCATGCTGCTCTGAAAGTTTTAGGTGATTCTCATGATTTGGAGCAATTCCCGGTACTCAAGGCAGAGCTTAGCATGAGACAACCGCTGTCTATTCTGGGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

990

Amino Acids

110.42

Weight (kDa)

6.01

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 538 - 723 1.7e-07 Type III restriction enzyme, res subunit
DEAD PF00270 559 - 727 4.8e-19 DEAD/DEAH box helicase
Helicase_C PF00271 769 - 882 1.1e-19 Helicase conserved C-terminal domain
RecG_dom3_C PF19833 909 - 966 1.2e-08 ATP-dependent DNA helicase RecG, domain 3, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 1465, 2433
AciI CCGC 5 cut(s) 494, 2402, 2433, 2445, 2948
AclWI GGATC 4 cut(s) 594, 1310, 1888, 2164
AcsI RAATTY 4 cut(s) 144, 1028, 1343, 1822
AcuI CTGAAG 2 cut(s) 1043, 1439
AfaI GTAC 8 cut(s) 118, 726, 1042, 1293, 1373, 1495, 1529, 2919
AfiI CCNNNNNNNGG 4 cut(s) 406, 1244, 1680, 2199
AflII CTTAAG 1 cut(s) 1181
AflIII ACRYGT 1 cut(s) 1043
AgeI ACCGGT 1 cut(s) 2248
AhdI GACNNNNNGTC 1 cut(s) 465
AjnI CCWGG 4 cut(s) 702, 727, 2236, 2770
AleI CACNNNNGTG 1 cut(s) 2537
AloI GAACNNNNNNTCC 2 cut(s) 1835, 1867
Alw21I GWGCWC 1 cut(s) 1815
Alw26I GTCTC 7 cut(s) 223, 358, 607, 1092, 1925, 2249, 2935
AlwI GGATC 4 cut(s) 594, 1310, 1888, 2164
AlwNI CAGNNNCTG 1 cut(s) 2399
AoxI GGCC 2 cut(s) 1220, 2190
ApoI RAATTY 4 cut(s) 144, 1028, 1343, 1822
AseI ATTAAT 1 cut(s) 750
AsiGI ACCGGT 1 cut(s) 2248
AspLEI GCGC 3 cut(s) 56, 1465, 1467
AspS9I GGNCC 8 cut(s) 467, 810, 1569, 1654, 2112, 2710, 2752, 2768
AsuC2I CCSGG 1 cut(s) 2915
AsuHPI GGTGA 5 cut(s) 1135, 1376, 1706, 2522, 2900
AsuNHI GCTAGC 3 cut(s) 20, 1459, 2092
AvaII GGWCC 8 cut(s) 467, 810, 1569, 1654, 2112, 2710, 2752, 2768
AxyI CCTNAGG 1 cut(s) 1658
BaeGI GKGCMC 1 cut(s) 1674
BbsI GAAGAC 2 cut(s) 266, 1352
Bbv12I GWGCWC 1 cut(s) 1815
BbvCI CCTCAGC 1 cut(s) 2705
BceAI ACGGC 1 cut(s) 2717
BcgI CGANNNNNNTGC 2 cut(s) 13, 47
BciT130I CCWGG 4 cut(s) 704, 729, 2238, 2772
BciVI GTATCC 2 cut(s) 555, 2361
BcnI CCSGG 1 cut(s) 2915
BcoDI GTCTC 7 cut(s) 223, 358, 607, 1092, 1925, 2249, 2935
BfaI CTAG 8 cut(s) 21, 758, 1460, 1520, 1730, 2093, 2312, 2345
BfmI CTRYAG 2 cut(s) 1716, 2437
BfrI CTTAAG 1 cut(s) 1181
BfuI GTATCC 2 cut(s) 555, 2361
BlpI GCTNAGC 1 cut(s) 2933
BmcAI AGTACT 1 cut(s) 118
Bme1390I CCNGG 5 cut(s) 704, 729, 2238, 2772, 2915
Bme18I GGWCC 8 cut(s) 467, 810, 1569, 1654, 2112, 2710, 2752, 2768
BmeRI GACNNNNNGTC 1 cut(s) 465
BmgT120I GGNCC 8 cut(s) 467, 810, 1569, 1654, 2112, 2710, 2752, 2768
BmiI GGNNCC 5 cut(s) 1655, 1779, 2113, 2139, 2711
BmrFI CCNGG 5 cut(s) 704, 729, 2238, 2772, 2915
BmsI GCATC 9 cut(s) 322, 1027, 1357, 2284, 2298, 2557, 2579, 2629, 2687
BmtI GCTAGC 3 cut(s) 24, 1463, 2096
BpiI GAAGAC 2 cut(s) 266, 1352
BplI GAGNNNNNCTC 4 cut(s) 504, 536, 602, 634
BpmI CTGGAG 2 cut(s) 1907, 1950
Bpu10I CCTNAGC 1 cut(s) 2705
Bpu1102I GCTNAGC 1 cut(s) 2933
BpuEI CTTGAG 3 cut(s) 387, 447, 2906
BpuMI CCSGG 1 cut(s) 2915
Bsa29I ATCGAT 1 cut(s) 226
BsaAI YACGTR 1 cut(s) 2765
BsaBI GATNNNNATC 1 cut(s) 376
BsaJI CCNNGG 5 cut(s) 636, 806, 2174, 2699, 2713
BsaWI WCCGGW 2 cut(s) 2248, 2353
BsaXI ACNNNNNCTCC 6 cut(s) 1089, 1119, 2057, 2087, 2322, 2352
Bsc4I CCNNNNNNNGG 4 cut(s) 406, 1244, 1680, 2199
Bse118I RCCGGY 2 cut(s) 905, 2248
Bse1I ACTGG 6 cut(s) 12, 629, 1620, 1667, 1933, 1959
Bse21I CCTNAGG 1 cut(s) 1658
Bse3DI GCAATG 1 cut(s) 342
Bse8I GATNNNNATC 1 cut(s) 376
BseBI CCWGG 4 cut(s) 704, 729, 2238, 2772
BseCI ATCGAT 1 cut(s) 226
BseDI CCNNGG 5 cut(s) 636, 806, 2174, 2699, 2713
BseJI GATNNNNATC 1 cut(s) 376
BseLI CCNNNNNNNGG 4 cut(s) 406, 1244, 1680, 2199
BseMI GCAATG 1 cut(s) 342
BseNI ACTGG 6 cut(s) 12, 629, 1620, 1667, 1933, 1959
BsePI GCGCGC 1 cut(s) 1463
BseRI GAGGAG 4 cut(s) 64, 203, 2130, 2661
BseSI GKGCMC 1 cut(s) 1674
BsgI GTGCAG 1 cut(s) 779
Bsh1236I CGCG 2 cut(s) 1465, 2433
BshFI GGCC 2 cut(s) 1222, 2192
BshTI ACCGGT 1 cut(s) 2248
BshVI ATCGAT 1 cut(s) 226
BsiHKAI GWGCWC 1 cut(s) 1815
BsiSI CCGG 4 cut(s) 906, 2249, 2354, 2915
BslFI GGGAC 5 cut(s) 436, 1667, 2125, 2546, 2814
BslI CCNNNNNNNGG 4 cut(s) 406, 1244, 1680, 2199
BsmAI GTCTC 7 cut(s) 223, 358, 607, 1092, 1925, 2249, 2935
BsmBI CGTCTC 1 cut(s) 223
BsmFI GGGAC 5 cut(s) 436, 1667, 2125, 2546, 2814
BsmI GAATGC 2 cut(s) 94, 835
BsnI GGCC 2 cut(s) 1222, 2192
Bsp1286I GDGCHC 2 cut(s) 1674, 1815
Bsp1407I TGTACA 1 cut(s) 1291
Bsp143I GATC 9 cut(s) 155, 223, 227, 514, 586, 1144, 1315, 1880, 2169
Bsp1720I GCTNAGC 1 cut(s) 2933
BspACI CCGC 5 cut(s) 494, 2402, 2433, 2445, 2948
BspANI GGCC 2 cut(s) 1222, 2192
BspDI ATCGAT 1 cut(s) 226
BspFNI CGCG 2 cut(s) 1465, 2433
BspHI TCATGA 3 cut(s) 277, 1363, 2896
BspLI GGNNCC 5 cut(s) 1655, 1779, 2113, 2139, 2711
BspOI GCTAGC 3 cut(s) 24, 1463, 2096
BspPI GGATC 4 cut(s) 594, 1310, 1888, 2164
BspQI GCTCTTC 1 cut(s) 1605
BspTI CTTAAG 1 cut(s) 1181
BsrDI GCAATG 1 cut(s) 342
BsrFI RCCGGY 2 cut(s) 905, 2248
BsrGI TGTACA 1 cut(s) 1291
BsrI ACTGG 6 cut(s) 12, 629, 1620, 1667, 1933, 1959
BssAI RCCGGY 2 cut(s) 905, 2248
BssECI CCNNGG 5 cut(s) 636, 806, 2174, 2699, 2713
BssHII GCGCGC 1 cut(s) 1463
BssMI GATC 9 cut(s) 155, 223, 227, 514, 586, 1144, 1315, 1880, 2169
BssT1I CCWWGG 1 cut(s) 2174
Bst2UI CCWGG 4 cut(s) 704, 729, 2238, 2772
Bst4CI ACNGT 8 cut(s) 781, 1062, 1717, 1976, 2049, 2057, 2673, 2796
Bst6I CTCTTC 3 cut(s) 1388, 1605, 2462
BstAFI CTTAAG 1 cut(s) 1181
BstAPI GCANNNNNTGC 3 cut(s) 1669, 2399, 2867
BstAUI TGTACA 1 cut(s) 1291
BstBAI YACGTR 1 cut(s) 2765
BstC8I GCNNGC 8 cut(s) 22, 214, 1461, 1465, 1735, 1794, 2094, 2696
BstDSI CCRYGG 2 cut(s) 636, 2699
BstENI CCTNNNNNAGG 1 cut(s) 1242
BstFNI CGCG 2 cut(s) 1465, 2433
BstHHI GCGC 3 cut(s) 56, 1465, 1467
BstKTI GATC 9 cut(s) 158, 226, 230, 517, 589, 1147, 1318, 1883, 2172
BstMAI GTCTC 7 cut(s) 223, 358, 607, 1092, 1925, 2249, 2935
BstMBI GATC 9 cut(s) 155, 223, 227, 514, 586, 1144, 1315, 1880, 2169
BstNI CCWGG 4 cut(s) 704, 729, 2238, 2772
BstNSI RCATGY 3 cut(s) 28, 1737, 2092
BstSCI CCNGG 5 cut(s) 702, 727, 2236, 2770, 2913
BstSFI CTRYAG 2 cut(s) 1716, 2437
BstSLI GKGCMC 1 cut(s) 1674
BstUI CGCG 2 cut(s) 1465, 2433
BstV2I GAAGAC 2 cut(s) 266, 1352
Bsu15I ATCGAT 1 cut(s) 226
Bsu36I CCTNAGG 1 cut(s) 1658
BsuI GTATCC 2 cut(s) 555, 2361
BsuRI GGCC 2 cut(s) 1222, 2192
BsuTUI ATCGAT 1 cut(s) 226
BtgI CCRYGG 2 cut(s) 636, 2699
BtsI GCAGTG 1 cut(s) 1629
BtsIMutI CAGTG 4 cut(s) 5, 1374, 1629, 1674
Cac8I GCNNGC 8 cut(s) 22, 214, 1461, 1465, 1735, 1794, 2094, 2696
CaiI CAGNNNCTG 1 cut(s) 2399
CciI TCATGA 3 cut(s) 277, 1363, 2896
CfoI GCGC 3 cut(s) 56, 1465, 1467
Cfr10I RCCGGY 2 cut(s) 905, 2248
Cfr13I GGNCC 8 cut(s) 467, 810, 1569, 1654, 2112, 2710, 2752, 2768
ClaI ATCGAT 1 cut(s) 226
CseI GACGC 1 cut(s) 205
CsiI ACCWGGT 1 cut(s) 2770
Csp6I GTAC 8 cut(s) 117, 725, 1041, 1292, 1372, 1494, 1528, 2918
CspAI ACCGGT 1 cut(s) 2248
CviQI GTAC 8 cut(s) 117, 725, 1041, 1292, 1372, 1494, 1528, 2918
DpnI GATC 9 cut(s) 157, 225, 229, 516, 588, 1146, 1317, 1882, 2171
DpnII GATC 9 cut(s) 155, 223, 227, 514, 586, 1144, 1315, 1880, 2169
DriI GACNNNNNGTC 1 cut(s) 465
Eam1104I CTCTTC 3 cut(s) 1388, 1605, 2462
Eam1105I GACNNNNNGTC 1 cut(s) 465
EarI CTCTTC 3 cut(s) 1388, 1605, 2462
Eco130I CCWWGG 1 cut(s) 2174
Eco32I GATATC 4 cut(s) 83, 1276, 1760, 1938
Eco47I GGWCC 8 cut(s) 467, 810, 1569, 1654, 2112, 2710, 2752, 2768
Eco57I CTGAAG 2 cut(s) 1043, 1439
Eco81I CCTNAGG 1 cut(s) 1658
EcoNI CCTNNNNNAGG 1 cut(s) 1242
EcoO109I RGGNCCY 3 cut(s) 1654, 2112, 2710
EcoRII CCWGG 4 cut(s) 702, 727, 2236, 2770
EcoRV GATATC 4 cut(s) 83, 1276, 1760, 1938
EcoT14I CCWWGG 1 cut(s) 2174
EcoT22I ATGCAT 3 cut(s) 94, 1739, 2572
ErhI CCWWGG 1 cut(s) 2174
Esp3I CGTCTC 1 cut(s) 223
FalI AAGNNNNNCTT 2 cut(s) 1537, 1569
FaqI GGGAC 5 cut(s) 436, 1667, 2125, 2546, 2814
FauNDI CATATG 2 cut(s) 1606, 2133
FspBI CTAG 8 cut(s) 21, 758, 1460, 1520, 1730, 2093, 2312, 2345
GlaI GCGC 3 cut(s) 55, 1464, 1466
GsuI CTGGAG 2 cut(s) 1907, 1950
HaeIII GGCC 2 cut(s) 1222, 2192
HapII CCGG 4 cut(s) 906, 2249, 2354, 2915
HgaI GACGC 1 cut(s) 205
HhaI GCGC 3 cut(s) 56, 1465, 1467
Hin6I GCGC 3 cut(s) 54, 1463, 1465
HinP1I GCGC 3 cut(s) 54, 1463, 1465
HincII GTYRAC 1 cut(s) 447
HindII GTYRAC 1 cut(s) 447
HpaII CCGG 4 cut(s) 906, 2249, 2354, 2915
HphI GGTGA 5 cut(s) 1135, 1376, 1706, 2522, 2900
Hpy166II GTNNAC 7 cut(s) 447, 725, 1043, 1374, 1496, 2669, 2768
Hpy8I GTNNAC 7 cut(s) 447, 725, 1043, 1374, 1496, 2669, 2768
HpyAV CCTTC 9 cut(s) 70, 467, 558, 993, 1248, 1463, 1478, 2321, 2765
HpyCH4III ACNGT 8 cut(s) 781, 1062, 1717, 1976, 2049, 2057, 2673, 2796
HpyCH4IV ACGT 2 cut(s) 1045, 2764
HpySE526I ACGT 2 cut(s) 1045, 2764
HspAI GCGC 3 cut(s) 54, 1463, 1465
Kzo9I GATC 9 cut(s) 155, 223, 227, 514, 586, 1144, 1315, 1880, 2169
LguI GCTCTTC 1 cut(s) 1605
LmnI GCTCC 3 cut(s) 51, 2143, 2905
LweI GCATC 9 cut(s) 322, 1027, 1357, 2284, 2298, 2557, 2579, 2629, 2687
MabI ACCWGGT 1 cut(s) 2770
MaeI CTAG 8 cut(s) 21, 758, 1460, 1520, 1730, 2093, 2312, 2345
MaeII ACGT 2 cut(s) 1045, 2764
MaeIII GTNAC 4 cut(s) 234, 1869, 2774, 2963
MalI GATC 9 cut(s) 157, 225, 229, 516, 588, 1146, 1317, 1882, 2171
MboI GATC 9 cut(s) 155, 223, 227, 514, 586, 1144, 1315, 1880, 2169
MhlI GDGCHC 2 cut(s) 1674, 1815
MlyI GAGTC 2 cut(s) 454, 1927
MmeI TCCRAC 1 cut(s) 2620
Mph1103I ATGCAT 3 cut(s) 94, 1739, 2572
MslI CAYNNNNRTG 2 cut(s) 522, 2537
MspA1I CMGCKG 3 cut(s) 2377, 2442, 2950
MspCI CTTAAG 1 cut(s) 1181
MspI CCGG 4 cut(s) 906, 2249, 2354, 2915
MspR9I CCNGG 5 cut(s) 704, 729, 2238, 2772, 2915
Mva1269I GAATGC 2 cut(s) 94, 835
MvaI CCWGG 4 cut(s) 704, 729, 2238, 2772
MvnI CGCG 2 cut(s) 1465, 2433
NciI CCSGG 1 cut(s) 2915
NdeI CATATG 2 cut(s) 1606, 2133
NdeII GATC 9 cut(s) 155, 223, 227, 514, 586, 1144, 1315, 1880, 2169
NheI GCTAGC 3 cut(s) 20, 1459, 2092
NlaIV GGNNCC 5 cut(s) 1655, 1779, 2113, 2139, 2711
NmuCI GTSAC 1 cut(s) 2963
NsiI ATGCAT 3 cut(s) 94, 1739, 2572
NspI RCATGY 3 cut(s) 28, 1737, 2092
OliI CACNNNNGTG 1 cut(s) 2537
PaeI GCATGC 1 cut(s) 1737
PagI TCATGA 3 cut(s) 277, 1363, 2896
PauI GCGCGC 1 cut(s) 1463
PciSI GCTCTTC 1 cut(s) 1605
PcsI WCGNNNNNNNCGW 2 cut(s) 1042, 2641
PctI GAATGC 2 cut(s) 94, 835
PfoI TCCNGGA 1 cut(s) 702
PinAI ACCGGT 1 cut(s) 2248
PleI GAGTC 2 cut(s) 454, 1926
PpsI GAGTC 2 cut(s) 454, 1926
Ppu21I YACGTR 1 cut(s) 2765
PpuMI RGGWCCY 3 cut(s) 1654, 2112, 2710
PshBI ATTAAT 1 cut(s) 750
Psp5II RGGWCCY 3 cut(s) 1654, 2112, 2710
Psp6I CCWGG 4 cut(s) 702, 727, 2236, 2770
PspGI CCWGG 4 cut(s) 702, 727, 2236, 2770
PspN4I GGNNCC 5 cut(s) 1655, 1779, 2113, 2139, 2711
PspPI GGNCC 8 cut(s) 467, 810, 1569, 1654, 2112, 2710, 2752, 2768
PspPPI RGGWCCY 3 cut(s) 1654, 2112, 2710
PstNI CAGNNNCTG 1 cut(s) 2399
PteI GCGCGC 1 cut(s) 1463
PvuII CAGCTG 2 cut(s) 2377, 2442
RsaI GTAC 8 cut(s) 118, 726, 1042, 1293, 1373, 1495, 1529, 2919
RsaNI GTAC 8 cut(s) 117, 725, 1041, 1292, 1372, 1494, 1528, 2918
RseI CAYNNNNRTG 2 cut(s) 522, 2537
SapI GCTCTTC 1 cut(s) 1605
Sau3AI GATC 9 cut(s) 155, 223, 227, 514, 586, 1144, 1315, 1880, 2169
Sau96I GGNCC 8 cut(s) 467, 810, 1569, 1654, 2112, 2710, 2752, 2768
ScaI AGTACT 1 cut(s) 118
SchI GAGTC 2 cut(s) 454, 1927
ScrFI CCNGG 5 cut(s) 704, 729, 2238, 2772, 2915
SduI GDGCHC 2 cut(s) 1674, 1815
SexAI ACCWGGT 1 cut(s) 2770
SfaNI GCATC 9 cut(s) 322, 1027, 1357, 2284, 2298, 2557, 2579, 2629, 2687
SfcI CTRYAG 2 cut(s) 1716, 2437
SinI GGWCC 8 cut(s) 467, 810, 1569, 1654, 2112, 2710, 2752, 2768
SmiMI CAYNNNNRTG 2 cut(s) 522, 2537
SmlI CTYRAG 4 cut(s) 402, 462, 1181, 2921
SmoI CTYRAG 4 cut(s) 402, 462, 1181, 2921
SphI GCATGC 1 cut(s) 1737
SsiI CCGC 5 cut(s) 494, 2402, 2433, 2445, 2948
SspI AATATT 5 cut(s) 138, 325, 1171, 1312, 1434
SspMI CTAG 8 cut(s) 21, 758, 1460, 1520, 1730, 2093, 2312, 2345
StyD4I CCNGG 5 cut(s) 702, 727, 2236, 2770, 2913
StyI CCWWGG 1 cut(s) 2174
TaaI ACNGT 8 cut(s) 781, 1062, 1717, 1976, 2049, 2057, 2673, 2796
TaiI ACGT 2 cut(s) 1048, 2767
TaqI TCGA 2 cut(s) 154, 226
TatI WGTACW 3 cut(s) 116, 1291, 1527
TauI GCSGC 1 cut(s) 2436
TscAI CASTG 4 cut(s) 12, 1381, 1636, 1674
TseFI GTSAC 1 cut(s) 2963
Tsp45I GTSAC 1 cut(s) 2963
TspGWI ACGGA 2 cut(s) 1918, 2011
TspRI CASTG 4 cut(s) 12, 1381, 1636, 1674
Vha464I CTTAAG 1 cut(s) 1181
VpaK11BI GGWCC 8 cut(s) 467, 810, 1569, 1654, 2112, 2710, 2752, 2768
VspI ATTAAT 1 cut(s) 750
XagI CCTNNNNNAGG 1 cut(s) 1242
XapI RAATTY 4 cut(s) 144, 1028, 1343, 1822
XceI RCATGY 3 cut(s) 28, 1737, 2092
XspI CTAG 8 cut(s) 21, 758, 1460, 1520, 1730, 2093, 2312, 2345
ZrmI AGTACT 1 cut(s) 118
Zsp2I ATGCAT 3 cut(s) 94, 1739, 2572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.