MD13G1025100.v1.1

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005436
Physical Location & Seq
Forward (+)
1763844 .. 1772171
8328 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1025100.v1.1.491

Sequence Viewer

Length: 2925 bp
ATGGCACCCACAGTCTCAGCTGTGCAACCTTGCTTCAGCGGGAATGGATTGAGAAGTGCAATTGCTTTTGAAGCTGAGAAGGGATATCGGAATGCTTTGGGTAGAAAAATGAGGTTCAGTAATTTTCTCTTTTCAACGGTATCGAGATTATGTTCTCGTCCAAAGCATAGTCTTGTCAAAAATAATTTGGAGGAAGTAGATATTTTTGGCATAGCAAGCATCTCAGATCGGTCAAAGTTGCTCAATAAGGTATCTGCTTTGGTTGGTTATGATAGTCTTGATGATTTGATTGAGAATGAAGGAGCTGAAAAGCAGTCCGGTATGCATGTTAGAGATGTCGTAGATGAATTTGATGTCTCCTTGGTGTGTGCACGATTTCCATCAATCATTTTAGGTAGTTCCCCTCGGGTGGAATTGTATGATGGAACCACCAGTTTCAATGAAAGGATGCTTTTGTCAACTCAAAGTGGTGAAGGATTTCTGTCTGATACTATGTGTGAAGAGCAGTCCGCAACTGTGGTTATTGAGGAAAACTCACATCAAACAGTTCCTGTGGAGGAATCTTCTAGCAAGGTTTTGCTTAAATCACAAAATCATACTGGGTCTGTTCAGTTTTCCCTGGACAGTTCTATCAGTTTCATACCTGGAATAACTAAGAAGCGCTGTCGGCAGCTAGAAGACTGTGGCTTTCATACATTGCGGCAATTGCTTCACCACTTTCCTCGGACCTATGCGGATTTACAGAATGCTCAGATGAAAATTGATGATGGACAGTACTTGATTTTCATTGGGAAGGTATTAAGTTCGAGGGGAATCAAAGCTAGTAGTTCTTTCTCATTTATTGAGGTAGTTGTTGGATGCGAAATTATGGACGATGAATCAACTGAGCATTTGAATGATTCTGCTGATGGTAGAAGAAAGAAGACAATTTCTTTGCATCTGAAGAAATTTTTTCGTGGCGCTCGTTTTACTTCTCTACCCTTTCTTAGACTTGTTGAGGGAAAGCATAAAGAGGGGGACTTTGTATGTGTTAGTGGCAAGGTAAGGACAATGCCTGTCAAAGGTCACTATGAAATGAGAGAATACAATATTGATGTGCTTAAAGATGAAAATGAAGTGTCTTTTCATGCAAAAGGGAGGCCTTATCCTATATACCCTTCAAAAGGAGGTTTAGATCCAAATGTTTTGAGAGACATCATTGCAAGAGTTGCACAAGTTTTGGCTGTCGATGTTGATCCTATTCCTAAAAATATCACTCAGGACTTTGGGCTGTTAAGCCTTCATGATGCGTACAAGGGGATTCACGAACCAAAGAGTATGGACGAAGCTGATTTGGCTCGGAAAAGGCTAATATTTGATGAGTTCTTTTACCTGCAGTTAGCGCGCCTTTACCAAATGCTTGAAGGTCTTGGTACACAAATAGAGAAAGATGGATTGCTGGATAAGTACAGGAAACCTGAATCAAGTGCTGCTTACACGAAGGAATGGTCCATTCTTACCAAGAAGTTTTCAAAAGCTCTTCCATATACCCTTACTCCTAGTCAACTCACTGCCGTTTCAGAAATCATCTGGGATCTAAGGCAACCAGTTCCTATGAATCGGCTTTTGCAGGTTGGTGATGTTGGTTGTGGGAAAACCGTGGTAGCTTTTCTAGCATGCATGGAGGTTATTGGCTCTGGATATCAGGCAGCTTTCATGGTTCCCACTGAGTTGCTTGCAGTCCAGCATTACGAGCACTTAAATAAATTGCTTGAAAATATGGAGGATGTTGAACGAAAACCTTCAATTGCTTTACTAACAGGCTCAACCCCATCAAAACAATCACGGATAATTCTTGAGGCATGGATTAATGTCCACTTCTTTTGTTTTGGTCTCAGGACTGGAGAAATATCGATGGTCGTTGGAACCACCAGTTTAATATCTGACAAAATCGAATTCTTTGCATTACGCATTGCAGTGGTGGATGAACAACACCGTTTTGGTGTGATCCAGAGAGGACGGTTTAACAGTAAGGTTCTATACTATACCTCCATAAATTCTAGAATGGTAGCCGCCAGTTCAGATGTGACCTCAAAAGGTGACAACCATATGGCTCCTCATGTTCTTGCCATGTCAGCTACTCCTATCCCGAGGACACTTGCTCTTGCTTTATATGGGGATATGTCATTGACACAGATAACAGACTTACCTCCAGGAAGAATTCCAGTTGAGACATTCATCATTGAAGGAAATGACAATGGATTTGAGGATGTTTATGAGATGATGCTGGATGAGTTAAAAGAAGGAGGGAGAGTCTATCTTGTGTATCCAGTTATTGAACAATCTGAACAGCTGCCGCAACTTCGTGCTGCTGCGGCAGATTTTGAAGCTATATCTGATAGGTTTCAGGGCTACACTTGTGGATTGTTGCATGGAAGGATGAAGAGTGATGAGAAAGATGAAGCATTGAGAAAGTTCAGATCTGGAGAAACAGATATACTGCTTTCCACACAAGTAATTGAGATTGGTGTTGATGTTCCGGATGCATCTATGATGGTTGTTATGAATGCTGAGAGATTTGGGATAGCTCAGTTACACCAACTTAGAGGACGAGTTGGACGAGGAGTGAGAAAGTCTAAATGTTTACTATTAGTATCTTCTTCCAGTAGTCTAACTCGGCTGAAGGTCCTGGGGAAATCGTCAGATGGTTTTTATTTGGCAAACATGGACCTTCTTTTACGTGGACCTGGTAACTTGCTTGGCAAGAAACAGTCAGGACATCTTCCAGAGTTTCCTATTGCAAGATTGGAAGTAGATGGCAATATCTTACAAGAAGCGCATCTTGCTGCTCTGAAAGTTTTAGGTGCTTCCCATGATTTGGAGCAATTCCCGCTGCTTAAGGCAGAGCTTAGCATGAGACAGCCGCTGTCTATTCTGGGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

975

Amino Acids

108.44

Weight (kDa)

6.25

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 510 - 708 1.4e-06 Type III restriction enzyme, res subunit
DEAD PF00270 533 - 712 8.9e-15 DEAD/DEAH box helicase
Helicase_C PF00271 753 - 867 1.7e-19 Helicase conserved C-terminal domain
RecG_dom3_C PF19833 894 - 950 4.5e-08 ATP-dependent DNA helicase RecG, domain 3, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1380, 1600
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 2857
AccII CGCG 1 cut(s) 1384
AccIII TCCGGA 1 cut(s) 2518
AciI CCGC 9 cut(s) 39, 510, 700, 734, 2052, 2336, 2354, 2870, 2903
AclWI GGATC 4 cut(s) 1169, 1229, 1581, 1981
AcsI RAATTY 5 cut(s) 347, 947, 1934, 2035, 2199
AcuI CTGAAG 3 cut(s) 19, 962, 2681
AfaI GTAC 4 cut(s) 776, 1292, 1414, 1448
AfeI AGCGCT 1 cut(s) 662
AfiI CCNNNNNNNGG 4 cut(s) 409, 1061, 1163, 2857
AflII CTTAAG 1 cut(s) 2876
AjnI CCWGG 5 cut(s) 618, 643, 2191, 2667, 2725
Alw21I GWGCWC 2 cut(s) 373, 1737
Alw26I GTCTC 6 cut(s) 19, 361, 1185, 1877, 2204, 2890
Alw44I GTGCAC 1 cut(s) 369
AlwI GGATC 4 cut(s) 1169, 1229, 1581, 1981
AlwNI CAGNNNCTG 2 cut(s) 551, 2905
Ama87I CYCGRG 2 cut(s) 405, 2128
Aor13HI TCCGGA 1 cut(s) 2518
Aor51HI AGCGCT 1 cut(s) 662
AoxI GGCC 1 cut(s) 1139
ApaLI GTGCAC 1 cut(s) 369
ApeKI GCWGC 8 cut(s) 670, 1469, 1688, 2332, 2348, 2351, 2825, 2872
ApoI RAATTY 5 cut(s) 347, 947, 1934, 2035, 2199
ArsI GACNNNNNNTTYG 6 cut(s) 916, 948, 1472, 1504, 2225, 2257
AseI ATTAAT 1 cut(s) 1848
Asp700I GAANNNNTTC 2 cut(s) 477, 1780
AspLEI GCGC 5 cut(s) 663, 962, 1384, 1386, 2818
AspS9I GGNCC 5 cut(s) 726, 1488, 2665, 2707, 2723
AsuHPI GGTGA 4 cut(s) 482, 704, 1628, 2090
AvaI CYCGRG 2 cut(s) 405, 2128
AvaII GGWCC 5 cut(s) 726, 1488, 2665, 2707, 2723
BaeGI GKGCMC 1 cut(s) 373
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 2 cut(s) 684, 929
Bbv12I GWGCWC 2 cut(s) 373, 1737
BbvI GCAGC 8 cut(s) 682, 1456, 1700, 2319, 2335, 2338, 2812, 2859
BceAI ACGGC 1 cut(s) 1538
BcgI CGANNNNNNTGC 2 cut(s) 1922, 1956
BciT130I CCWGG 5 cut(s) 620, 645, 2193, 2669, 2727
BciVI GTATCC 1 cut(s) 2316
BcoDI GTCTC 6 cut(s) 19, 361, 1185, 1877, 2204, 2890
BfaI CTAG 6 cut(s) 567, 674, 822, 1539, 1652, 2040
BfmI CTRYAG 1 cut(s) 1373
BfoI RGCGCY 2 cut(s) 664, 963
BfrI CTTAAG 1 cut(s) 2876
BfuAI ACCTGC 2 cut(s) 1380, 1600
BfuI GTATCC 1 cut(s) 2316
BglII AGATCT 1 cut(s) 2459
BlpI GCTNAGC 1 cut(s) 2888
BmcAI AGTACT 1 cut(s) 776
Bme1390I CCNGG 5 cut(s) 620, 645, 2193, 2669, 2727
Bme18I GGWCC 5 cut(s) 726, 1488, 2665, 2707, 2723
BmeT110I CYCGRG 2 cut(s) 405, 2128
BmgT120I GGNCC 5 cut(s) 726, 1488, 2665, 2707, 2723
BmiI GGNNCC 5 cut(s) 6, 427, 1701, 1906, 2094
BmrFI CCNGG 5 cut(s) 620, 645, 2193, 2669, 2727
BmrI ACTGGG 1 cut(s) 609
BmsI GCATC 9 cut(s) 228, 438, 848, 946, 1276, 2253, 2512, 2534, 2827
BmuI ACTGGG 1 cut(s) 609
BpiI GAAGAC 2 cut(s) 684, 929
BplI GAGNNNNNCTC 2 cut(s) 518, 550
BpmI CTGGAG 3 cut(s) 1902, 2175, 2484
Bpu1102I GCTNAGC 1 cut(s) 2888
BpuEI CTTGAG 1 cut(s) 1856
Bsa29I ATCGAT 1 cut(s) 1892
BsaAI YACGTR 1 cut(s) 2720
BsaBI GATNNNNATC 2 cut(s) 379, 1233
BsaI GGTCTC 1 cut(s) 1877
BsaJI CCNNGG 7 cut(s) 360, 404, 618, 722, 1638, 2129, 2668
BsaWI WCCGGW 2 cut(s) 317, 2518
BsaXI ACNNNNNCTCC 6 cut(s) 1519, 1549, 2012, 2042, 2277, 2307
Bsc4I CCNNNNNNNGG 4 cut(s) 409, 1061, 1163, 2857
Bse1I ACTGG 9 cut(s) 432, 604, 1586, 1885, 1911, 2055, 2204, 2309, 2643
Bse3DI GCAATG 3 cut(s) 695, 1197, 1950
Bse8I GATNNNNATC 2 cut(s) 379, 1233
BseAI TCCGGA 1 cut(s) 2518
BseBI CCWGG 5 cut(s) 620, 645, 2193, 2669, 2727
BseCI ATCGAT 1 cut(s) 1892
BseDI CCNNGG 7 cut(s) 360, 404, 618, 722, 1638, 2129, 2668
BseGI GGATG 8 cut(s) 453, 863, 1771, 1969, 2254, 2275, 2424, 2527
BseJI GATNNNNATC 2 cut(s) 379, 1233
BseLI CCNNNNNNNGG 4 cut(s) 409, 1061, 1163, 2857
BseMI GCAATG 3 cut(s) 695, 1197, 1950
BseNI ACTGG 9 cut(s) 432, 604, 1586, 1885, 1911, 2055, 2204, 2309, 2643
BsePI GCGCGC 1 cut(s) 1382
BseRI GAGGAG 2 cut(s) 2085, 2616
BseSI GKGCMC 1 cut(s) 373
BseXI GCAGC 8 cut(s) 682, 1456, 1700, 2319, 2335, 2338, 2812, 2859
Bsh1236I CGCG 1 cut(s) 1384
BshFI GGCC 1 cut(s) 1141
BshNI GGYRCC 1 cut(s) 4
BshVI ATCGAT 1 cut(s) 1892
BsiHKAI GWGCWC 2 cut(s) 373, 1737
BsiHKCI CYCGRG 2 cut(s) 405, 2128
BsiSI CCGG 2 cut(s) 318, 2519
BslFI GGGAC 1 cut(s) 1031
BslI CCNNNNNNNGG 4 cut(s) 409, 1061, 1163, 2857
BsmAI GTCTC 6 cut(s) 19, 361, 1185, 1877, 2204, 2890
BsmFI GGGAC 1 cut(s) 1031
BsmI GAATGC 3 cut(s) 97, 751, 2551
BsnI GGCC 1 cut(s) 1141
Bso31I GGTCTC 1 cut(s) 1877
BsoBI CYCGRG 2 cut(s) 405, 2128
Bsp1286I GDGCHC 2 cut(s) 373, 1737
Bsp13I TCCGGA 1 cut(s) 2518
Bsp143I GATC 6 cut(s) 226, 1174, 1234, 1573, 1986, 2459
Bsp1720I GCTNAGC 1 cut(s) 2888
BspACI CCGC 9 cut(s) 39, 510, 700, 734, 2052, 2336, 2354, 2870, 2903
BspANI GGCC 1 cut(s) 1141
BspDI ATCGAT 1 cut(s) 1892
BspEI TCCGGA 1 cut(s) 2518
BspFNI CGCG 1 cut(s) 1384
BspHI TCATGA 1 cut(s) 1282
BspLI GGNNCC 5 cut(s) 6, 427, 1701, 1906, 2094
BspMAI CTGCAG 1 cut(s) 1377
BspMI ACCTGC 2 cut(s) 1380, 1600
BspPI GGATC 4 cut(s) 1169, 1229, 1581, 1981
BspQI GCTCTTC 2 cut(s) 495, 1524
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 2876
BspTNI GGTCTC 1 cut(s) 1877
BsrDI GCAATG 3 cut(s) 695, 1197, 1950
BsrI ACTGG 9 cut(s) 432, 604, 1586, 1885, 1911, 2055, 2204, 2309, 2643
BssECI CCNNGG 7 cut(s) 360, 404, 618, 722, 1638, 2129, 2668
BssHII GCGCGC 1 cut(s) 1382
BssMI GATC 6 cut(s) 226, 1174, 1234, 1573, 1986, 2459
BssT1I CCWWGG 1 cut(s) 360
Bst2UI CCWGG 5 cut(s) 620, 645, 2193, 2669, 2727
Bst6I CTCTTC 3 cut(s) 495, 1524, 2417
BstAFI CTTAAG 1 cut(s) 2876
BstAPI GCANNNNNTGC 1 cut(s) 1208
BstBAI YACGTR 1 cut(s) 2720
BstC8I GCNNGC 4 cut(s) 217, 1384, 1657, 1716
BstDSI CCRYGG 1 cut(s) 1638
BstENI CCTNNNNNAGG 2 cut(s) 1059, 1161
BstF5I GGATG 8 cut(s) 453, 863, 1771, 1969, 2254, 2275, 2424, 2527
BstFNI CGCG 1 cut(s) 1384
BstH2I RGCGCY 2 cut(s) 664, 963
BstHHI GCGC 5 cut(s) 663, 962, 1384, 1386, 2818
BstKTI GATC 6 cut(s) 229, 1177, 1237, 1576, 1989, 2462
BstMAI GTCTC 6 cut(s) 19, 361, 1185, 1877, 2204, 2890
BstMBI GATC 6 cut(s) 226, 1174, 1234, 1573, 1986, 2459
BstNI CCWGG 5 cut(s) 620, 645, 2193, 2669, 2727
BstNSI RCATGY 2 cut(s) 329, 1659
BstSCI CCNGG 5 cut(s) 618, 643, 2191, 2667, 2725
BstSFI CTRYAG 1 cut(s) 1373
BstSLI GKGCMC 1 cut(s) 373
BstUI CGCG 1 cut(s) 1384
BstV1I GCAGC 8 cut(s) 682, 1456, 1700, 2319, 2335, 2338, 2812, 2859
BstV2I GAAGAC 2 cut(s) 684, 929
BstX2I RGATCY 3 cut(s) 1174, 1573, 2459
BstYI RGATCY 3 cut(s) 1174, 1573, 2459
Bsu15I ATCGAT 1 cut(s) 1892
BsuI GTATCC 1 cut(s) 2316
BsuRI GGCC 1 cut(s) 1141
BsuTUI ATCGAT 1 cut(s) 1892
BtgI CCRYGG 1 cut(s) 1638
BtsCI GGATG 8 cut(s) 453, 863, 1771, 1969, 2254, 2275, 2424, 2527
BtsI GCAGTG 2 cut(s) 1548, 1962
BtsIMutI CAGTG 3 cut(s) 1548, 1704, 1962
BveI ACCTGC 2 cut(s) 1380, 1600
Cac8I GCNNGC 4 cut(s) 217, 1384, 1657, 1716
CaiI CAGNNNCTG 2 cut(s) 551, 2905
CciI TCATGA 1 cut(s) 1282
CfoI GCGC 5 cut(s) 663, 962, 1384, 1386, 2818
Cfr13I GGNCC 5 cut(s) 726, 1488, 2665, 2707, 2723
ClaI ATCGAT 1 cut(s) 1892
CsiI ACCWGGT 1 cut(s) 2725
Csp6I GTAC 4 cut(s) 775, 1291, 1413, 1447
CspCI CAANNNNNGTGG 2 cut(s) 1844, 1879
CviQI GTAC 4 cut(s) 775, 1291, 1413, 1447
DpnI GATC 6 cut(s) 228, 1176, 1236, 1575, 1988, 2461
DpnII GATC 6 cut(s) 226, 1174, 1234, 1573, 1986, 2459
Eam1104I CTCTTC 3 cut(s) 495, 1524, 2417
EarI CTCTTC 3 cut(s) 495, 1524, 2417
Eco130I CCWWGG 1 cut(s) 360
Eco147I AGGCCT 1 cut(s) 1141
Eco31I GGTCTC 1 cut(s) 1877
Eco32I GATATC 2 cut(s) 86, 1682
Eco47I GGWCC 5 cut(s) 726, 1488, 2665, 2707, 2723
Eco47III AGCGCT 1 cut(s) 662
Eco57I CTGAAG 3 cut(s) 19, 962, 2681
Eco88I CYCGRG 2 cut(s) 405, 2128
EcoNI CCTNNNNNAGG 2 cut(s) 1059, 1161
EcoO109I RGGNCCY 1 cut(s) 2665
EcoRI GAATTC 2 cut(s) 1934, 2199
EcoRII CCWGG 5 cut(s) 618, 643, 2191, 2667, 2725
EcoRV GATATC 2 cut(s) 86, 1682
EcoT14I CCWWGG 1 cut(s) 360
EcoT22I ATGCAT 3 cut(s) 327, 1661, 2527
ErhI CCWWGG 1 cut(s) 360
FalI AAGNNNNNCTT 4 cut(s) 1456, 1488, 2805, 2837
FaqI GGGAC 1 cut(s) 1031
FauI CCCGC 2 cut(s) 32, 2877
FauNDI CATATG 1 cut(s) 2088
FokI GGATG 8 cut(s) 460, 870, 1778, 1976, 2261, 2282, 2431, 2534
FspBI CTAG 6 cut(s) 567, 674, 822, 1539, 1652, 2040
GlaI GCGC 5 cut(s) 662, 961, 1383, 1385, 2817
GsuI CTGGAG 3 cut(s) 1902, 2175, 2484
HaeII RGCGCY 2 cut(s) 664, 963
HaeIII GGCC 1 cut(s) 1141
HapII CCGG 2 cut(s) 318, 2519
HhaI GCGC 5 cut(s) 663, 962, 1384, 1386, 2818
Hin6I GCGC 5 cut(s) 661, 960, 1382, 1384, 2816
HinP1I GCGC 5 cut(s) 661, 960, 1382, 1384, 2816
HincII GTYRAC 2 cut(s) 459, 1544
HindII GTYRAC 2 cut(s) 459, 1544
HinfI GANTC 8 cut(s) 560, 813, 878, 899, 1300, 1460, 1597, 2292
HpaII CCGG 2 cut(s) 318, 2519
HphI GGTGA 4 cut(s) 482, 704, 1628, 2090
Hpy166II GTNNAC 7 cut(s) 371, 459, 1415, 1544, 1855, 2624, 2723
Hpy8I GTNNAC 7 cut(s) 371, 459, 1415, 1544, 1855, 2624, 2723
HpyCH4IV ACGT 1 cut(s) 2719
HpySE526I ACGT 1 cut(s) 2719
HspAI GCGC 5 cut(s) 661, 960, 1382, 1384, 2816
Kpn2I TCCGGA 1 cut(s) 2518
Kzo9I GATC 6 cut(s) 226, 1174, 1234, 1573, 1986, 2459
LguI GCTCTTC 2 cut(s) 495, 1524
LmnI GCTCC 3 cut(s) 302, 2098, 2860
Lsp1109I GCAGC 8 cut(s) 682, 1456, 1700, 2319, 2335, 2338, 2812, 2859
LweI GCATC 9 cut(s) 228, 438, 848, 946, 1276, 2253, 2512, 2534, 2827
MabI ACCWGGT 1 cut(s) 2725
MaeI CTAG 6 cut(s) 567, 674, 822, 1539, 1652, 2040
MaeII ACGT 1 cut(s) 2719
MaeIII GTNAC 6 cut(s) 1064, 2065, 2078, 2571, 2729, 2918
MalI GATC 6 cut(s) 228, 1176, 1236, 1575, 1988, 2461
MboI GATC 6 cut(s) 226, 1174, 1234, 1573, 1986, 2459
MfeI CAATTG 3 cut(s) 60, 704, 1785
MflI RGATCY 3 cut(s) 1174, 1573, 2459
MhlI GDGCHC 2 cut(s) 373, 1737
MlyI GAGTC 1 cut(s) 2301
MmeI TCCRAC 3 cut(s) 835, 1882, 2575
Mph1103I ATGCAT 3 cut(s) 327, 1661, 2527
MroI TCCGGA 1 cut(s) 2518
MroXI GAANNNNTTC 2 cut(s) 477, 1780
MslI CAYNNNNRTG 2 cut(s) 894, 1955
MspA1I CMGCKG 5 cut(s) 20, 39, 2332, 2872, 2905
MspCI CTTAAG 1 cut(s) 2876
MspI CCGG 2 cut(s) 318, 2519
MspR9I CCNGG 5 cut(s) 620, 645, 2193, 2669, 2727
MunI CAATTG 3 cut(s) 60, 704, 1785
Mva1269I GAATGC 3 cut(s) 97, 751, 2551
MvaI CCWGG 5 cut(s) 620, 645, 2193, 2669, 2727
MvnI CGCG 1 cut(s) 1384
NdeI CATATG 1 cut(s) 2088
NdeII GATC 6 cut(s) 226, 1174, 1234, 1573, 1986, 2459
NlaIV GGNNCC 5 cut(s) 6, 427, 1701, 1906, 2094
NmeAIII GCCGAG 1 cut(s) 2635
NmuCI GTSAC 4 cut(s) 1064, 2065, 2078, 2918
NsiI ATGCAT 3 cut(s) 327, 1661, 2527
NspI RCATGY 2 cut(s) 329, 1659
PaeI GCATGC 1 cut(s) 1659
PagI TCATGA 1 cut(s) 1282
PauI GCGCGC 1 cut(s) 1382
PceI AGGCCT 1 cut(s) 1141
PciSI GCTCTTC 2 cut(s) 495, 1524
PcsI WCGNNNNNNNCGW 2 cut(s) 961, 2596
PctI GAATGC 3 cut(s) 97, 751, 2551
PdmI GAANNNNTTC 2 cut(s) 477, 1780
PfeI GAWTC 7 cut(s) 560, 813, 878, 899, 1300, 1460, 1597
PflMI CCANNNNNTGG 1 cut(s) 2857
PfoI TCCNGGA 1 cut(s) 2191
PleI GAGTC 1 cut(s) 2300
PpsI GAGTC 1 cut(s) 2300
Ppu21I YACGTR 1 cut(s) 2720
PpuMI RGGWCCY 1 cut(s) 2665
PshBI ATTAAT 1 cut(s) 1848
Psp5II RGGWCCY 1 cut(s) 2665
Psp6I CCWGG 5 cut(s) 618, 643, 2191, 2667, 2725
PspGI CCWGG 5 cut(s) 618, 643, 2191, 2667, 2725
PspN4I GGNNCC 5 cut(s) 6, 427, 1701, 1906, 2094
PspPI GGNCC 5 cut(s) 726, 1488, 2665, 2707, 2723
PspPPI RGGWCCY 1 cut(s) 2665
PstI CTGCAG 1 cut(s) 1377
PstNI CAGNNNCTG 2 cut(s) 551, 2905
PsuI RGATCY 3 cut(s) 1174, 1573, 2459
PteI GCGCGC 1 cut(s) 1382
PvuII CAGCTG 2 cut(s) 20, 2332
RsaI GTAC 4 cut(s) 776, 1292, 1414, 1448
RsaNI GTAC 4 cut(s) 775, 1291, 1413, 1447
RseI CAYNNNNRTG 2 cut(s) 894, 1955
SapI GCTCTTC 2 cut(s) 495, 1524
Sau3AI GATC 6 cut(s) 226, 1174, 1234, 1573, 1986, 2459
Sau96I GGNCC 5 cut(s) 726, 1488, 2665, 2707, 2723
ScaI AGTACT 1 cut(s) 776
SchI GAGTC 1 cut(s) 2301
ScrFI CCNGG 5 cut(s) 620, 645, 2193, 2669, 2727
SduI GDGCHC 2 cut(s) 373, 1737
SexAI ACCWGGT 1 cut(s) 2725
SfaNI GCATC 9 cut(s) 228, 438, 848, 946, 1276, 2253, 2512, 2534, 2827
SfcI CTRYAG 1 cut(s) 1373
SinI GGWCC 5 cut(s) 726, 1488, 2665, 2707, 2723
SmiMI CAYNNNNRTG 2 cut(s) 894, 1955
SmlI CTYRAG 2 cut(s) 1835, 2876
SmoI CTYRAG 2 cut(s) 1835, 2876
SphI GCATGC 1 cut(s) 1659
SseBI AGGCCT 1 cut(s) 1141
SsiI CCGC 9 cut(s) 39, 510, 700, 734, 2052, 2336, 2354, 2870, 2903
SspI AATATT 2 cut(s) 1090, 1353
SspMI CTAG 6 cut(s) 567, 674, 822, 1539, 1652, 2040
StuI AGGCCT 1 cut(s) 1141
StyD4I CCNGG 5 cut(s) 618, 643, 2191, 2667, 2725
StyI CCWWGG 1 cut(s) 360
TaiI ACGT 1 cut(s) 2722
TaqI TCGA 5 cut(s) 143, 806, 1227, 1892, 1932
TaqII GACCGA 1 cut(s) 219
TatI WGTACW 2 cut(s) 774, 1446
TauI GCSGC 5 cut(s) 703, 2054, 2338, 2357, 2905
TfiI GAWTC 7 cut(s) 560, 813, 878, 899, 1300, 1460, 1597
TscAI CASTG 3 cut(s) 1555, 1711, 1962
TseFI GTSAC 4 cut(s) 1064, 2065, 2078, 2918
TseI GCWGC 8 cut(s) 670, 1469, 1688, 2332, 2348, 2351, 2825, 2872
Tsp45I GTSAC 4 cut(s) 1064, 2065, 2078, 2918
TspGWI ACGGA 1 cut(s) 1840
TspRI CASTG 3 cut(s) 1555, 1711, 1962
Van91I CCANNNNNTGG 1 cut(s) 2857
Vha464I CTTAAG 1 cut(s) 2876
VneI GTGCAC 1 cut(s) 369
VpaK11BI GGWCC 5 cut(s) 726, 1488, 2665, 2707, 2723
VspI ATTAAT 1 cut(s) 1848
XagI CCTNNNNNAGG 2 cut(s) 1059, 1161
XapI RAATTY 5 cut(s) 347, 947, 1934, 2035, 2199
XbaI TCTAGA 1 cut(s) 2039
XceI RCATGY 2 cut(s) 329, 1659
XmnI GAANNNNTTC 2 cut(s) 477, 1780
XspI CTAG 6 cut(s) 567, 674, 822, 1539, 1652, 2040
ZrmI AGTACT 1 cut(s) 776
Zsp2I ATGCAT 3 cut(s) 327, 1661, 2527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.