Rroxscaffold_5G00382220

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005436
Physical Location & Seq
Reverse (-)
62067446 .. 62079768
12323 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00382220.1

Sequence Viewer

Length: 3198 bp
ATGGCACTGGCAGTTTCAGCTGGCATGTGTTTCGGTGGAAATGGATTGAGAAGTGCAATAGCTTTTGAAGCTGAGAAGGGATATCGGAATGCATTGGGTAGCAAAATGAGGTTCAGTACTTTTCTCCTTTCCAAAGTATTGAAATTATGTTCTCGATCAAAGCATGCTTTTGCTAAGAGTATTTTGGAGGAGGCAGATAGTTATGGCATAGCAAGCATCTCAGATCGGTCAAAGTTGCTTAACAAGAGGCTTGCTGTTGAATACCCATTCCCTGGCATAGCTCTTCCAGTTGGAAATTCTAAGTTCTATATCAGCTATATGGTTATGAAGAGAAATGTGGCTCCTTACATTATTAGAAAGTGGCTTTTAAGTGAGCTATTTCAGATTGTCTTTTGTGAAGCCCGAATTCCCTGGTGTCCTGGATCTCTCAAATCCATCACTGTGCATTTTAGAACAAGATTTAGTATAAGGAATGCTGTATCTGTTTTGATGGGTTATGATGGTCTTCATGATTTAATTGAGAATGAAAGAGCTGAAAAGCATTATGGTATGAATTTTAAAGATGCAATGGATGATTTTGATGCCTCCTTTGTCTGCAAGAGATTTCCATCAATCATTTTGGGTGATTCCCCTCCGGTGGAATTGTATGATGGGACTGCTAATTTCTTTGAAAAAATGACACTTTTAACAACTCAAAGTCCTGAAGGATTTCCTTCTGGCTCTGCGGTTGAAGAGCAGGAGGGAGATTATCTCTATGAAACAGGGGATTCTCTGTATCCTGAGTTTCCAAGTGAAGAAACTTCCTTGTTAACAGATGATCAGTCCACGACTTTGGCTACTGAGGGATACTCATATCAACCAGTTCCTGTGGAGGAATCTTCTAACAAAGTTTCGCATAAATCACAAAAGAATGTGCCTGATGAGGTGTTCCTGGACAATTCTATCAGTTGTATACCTGGAATAAGTAAGAAGCGTCTCAATCAGCTAGAAAATTGTGGCTTTCATACGGTGCGGAAATTGCTGCACCACTTTCCTCGGACCTACGCTGATTTACAGAATGCTCAAATGGACATAGATGATGGACAGTACTTGATTTTCATTGGAAAGATATTAAAATCAAGGGGAATCAAAGCTGGTACTTCTTTTTCAATTGTTGAGGTTGTCGTGGGTTGTGAAATTGCTGACGATAAAATAACCGAGCGTAAGGATGATTCTACAGATTGTAGAAGGAAGACAATATATTTGCATCTGAAGAAATTTTTTCGTGGTACACGTTTTACTTCTCTACCGTTTCTTAGAATAGTTGAGAAGAAGCACAGAGAGGGAGACTTTGTATGTGTTAGTGGTAAGGTGAGGATAATGCCTGCCAAAGATCACTATGAAATGAGAGAATATAATATTGATGTCCTTAAAGATGAAAATGACTTGTCTTTTCATGCAAAAGGGAGGCCATATCCTATATACCCTTCAAAAGGAGGCTTAAATCCTGACGTTTTGAGAGATATCATTGCAAGAGTTGTACAAGTTTTACCTATCAATATTGATCCCATTCCTAAAAGAATCACTCTGGAATTTGGGCTGCTCAGTCTTCATGATGCTTATACCGAGATTCACCAACCGAAGAGTATGAGTGAAGCTGATTTGGCTCGGAAGAGGCTAATATTTGATGAGTTCTTTTACCTTCAGCTAGCGCGCTTATACCAAATGCTTGAAGGGCTTGGTACACAAATAGAGAAAGATGGATTGCTAAATAAGTATAGGAAACCTGAATCAAGTGCTACTTATATGGAAGACTGGTCCAGTCTTACCAAGAAGTTTCTAAAAGCTCTTCCATATGCCCTTACGGGTAGTCAACTCACTGCTGTTTCAGAAATTATCTGGGATCTCAGGCAACCAGTTCCAATGAATCGGCTTTTGCAGGGTGATGTTGGCTGTGGTAAAACTGTAGTTGCTTTTCTAGCATGCATGGAGGTAATGGGCTCTGGATATCAGGCAGCTTTCATGGTTCCTACTGAGTTGCTTGCTGTCCAGCATTACGAGCACTTGAAGAATTTGCTTGAAACTATGGAGGATGTTGAGTGCAAACCTTCTATTGCCTTACTAACAGGATCAACCCCGTCAAAACAGTCACGGATGATTCAAAAGGCAAGTAATAACACTGGAGAGATATCAATGGTCATTGGAACGACTAGTTTAATAGCTGACCGTGTGGAGTTCTCTGCATTACGGATTGCTGTGGTGGATGAACAACATCGTTTTGGTGTCATCCAGAGAGGACGGTTTAACAGTAAGCTATACTATACCTCCATAATATCTAACATGCTAGCCACAAACTCAGATGGGACCTCCAAAGGTGAAAAGCATATGGCTCCTCATGTTCTTGCCATGTCAGCTACTCCGATCCCAAGGACGCTTGCTCTGGCTTTATATGGGGATATGTCATTAACACAAATAACAGACTTGCCACCAGGAAGAATACCAGTGGAGACATTCATTATTCAAGGACATGAAAATGGGTATGAGGATGCATATGAGATGATGCTCGATGAGTTAAAAGAAGGAGGCAAAGTGTATCTAGTGTATCCGGTCATTGAACAATCTGAGCAGCTGCCTCAACTTCGTGCAGCTTCTGCAGATTTTGAAGCTATATCTCATAGGTTTCAGGGCTACAGCTGTGGATTGTTGCATGGAAAAATGAAGAGTGATGAGAAAGATGAAGCACTGAGAAAGTTTAGGTCTGGAGAAACAAATATACTTCTTGCCACACAAGTAATTGAGATTGGTGTTGATGTCCCAGATGCATCTATGATGGTTGTTATGAATGCTGAGAGGTTTGGGATTGCTCAGTTACATCAGCTTAGAGGACGAGTTGGACGAGGTGTGAGAAAGTCTAAGTGTATACTGTTAGCATCTTCTGAGAGTAGCCTACCACGGCTGAAGGTCCTTGGGAAATCATCAGATGGGTTTTACTTGGCGAATATGGACCTTCTTTTACGTGGACCTGGTAACTTGCTTGGCAAGAAACAGTCGGGACATCTTCCAGAGTTTCCTATTGCAAGATTGGAAATAGATGGCAATATCTTACAAGAAGCACACCTTGCTGCTCTGAAAATTTTAGGTGATTCTCATGATTTGGAGCAATTCCCACTGCTCAAAGCAGAGCTTAGCATGAGACAGCCTCTGTCTATTCTGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

1065

Amino Acids

119.23

Weight (kDa)

6.72

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 617 - 800 4.4e-07 Type III restriction enzyme, res subunit
DEAD PF00270 636 - 804 5.7e-19 DEAD/DEAH box helicase
Helicase_C PF00271 846 - 959 1.1e-19 Helicase conserved C-terminal domain
RecG_dom3_C PF19833 986 - 1042 4.5e-08 ATP-dependent DNA helicase RecG, domain 3, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 272
AccI GTMKAC 2 cut(s) 952, 2899
AccII CGCG 1 cut(s) 1693
AciI CCGC 2 cut(s) 725, 1012
AclWI GGATC 5 cut(s) 430, 1538, 1890, 2116, 2395
AcsI RAATTY 7 cut(s) 295, 405, 553, 1256, 1571, 2050, 3111
AcuI CTGAAG 4 cut(s) 723, 1271, 1667, 2957
AfaI GTAC 6 cut(s) 118, 1088, 1138, 1270, 1521, 1723
AfiI CCNNNNNNNGG 3 cut(s) 272, 637, 1472
AflIII ACRYGT 1 cut(s) 1271
AhlI ACTAGT 1 cut(s) 2189
AjnI CCWGG 7 cut(s) 271, 410, 418, 930, 955, 2467, 3001
Alw21I GWGCWC 1 cut(s) 2043
Alw26I GTCTC 4 cut(s) 980, 1320, 2480, 3166
AlwI GGATC 5 cut(s) 430, 1538, 1890, 2116, 2395
AlwNI CAGNNNCTG 3 cut(s) 866, 2630, 3181
AoxI GGCC 1 cut(s) 1448
ApeKI GCWGC 7 cut(s) 1021, 1579, 1994, 2605, 2608, 2624, 3101
ApoI RAATTY 7 cut(s) 295, 405, 553, 1256, 1571, 2050, 3111
ArsI GACNNNNNNTTYG 2 cut(s) 1225, 1257
Asp700I GAANNNNTTC 1 cut(s) 708
AspLEI GCGC 2 cut(s) 1693, 1695
AspS9I GGNCC 6 cut(s) 1038, 1797, 2343, 2941, 2983, 2999
AsuHPI GGTGA 6 cut(s) 635, 1363, 1604, 1934, 2366, 3131
AsuNHI GCTAGC 2 cut(s) 1687, 2323
AvaII GGWCC 6 cut(s) 1038, 1797, 2343, 2941, 2983, 2999
BanII GRGCYC 1 cut(s) 1982
BbsI GAAGAC 4 cut(s) 497, 1238, 1580, 1797
Bbv12I GWGCWC 1 cut(s) 2043
BbvI GCAGC 7 cut(s) 1008, 1566, 2006, 2595, 2617, 2636, 3088
BceAI ACGGC 1 cut(s) 2948
BcgI CGANNNNNNTGC 2 cut(s) 13, 47
BciT130I CCWGG 7 cut(s) 273, 412, 420, 932, 957, 2469, 3003
BciVI GTATCC 3 cut(s) 786, 839, 2592
BclI TGATCA 1 cut(s) 817
BcoDI GTCTC 4 cut(s) 980, 1320, 2480, 3166
BcuI ACTAGT 1 cut(s) 2189
BfaI CTAG 6 cut(s) 986, 1688, 1958, 2190, 2324, 2576
BfmI CTRYAG 4 cut(s) 1215, 1944, 2631, 2668
BfuI GTATCC 3 cut(s) 786, 839, 2592
BisI GCNGC 7 cut(s) 1022, 1580, 1995, 2606, 2609, 2625, 3102
BlpI GCTNAGC 1 cut(s) 3164
BlsI GCNGC 7 cut(s) 1023, 1581, 1996, 2607, 2610, 2626, 3103
BmcAI AGTACT 2 cut(s) 118, 1088
Bme1390I CCNGG 7 cut(s) 273, 412, 420, 932, 957, 2469, 3003
Bme18I GGWCC 6 cut(s) 1038, 1797, 2343, 2941, 2983, 2999
BmgT120I GGNCC 6 cut(s) 1038, 1797, 2343, 2941, 2983, 2999
BmiI GGNNCC 4 cut(s) 342, 2007, 2344, 2370
BmrFI CCNGG 7 cut(s) 273, 412, 420, 932, 957, 2469, 3003
BmtI GCTAGC 2 cut(s) 1691, 2327
BpiI GAAGAC 4 cut(s) 497, 1238, 1580, 1797
BplI GAGNNNNNCTC 6 cut(s) 735, 767, 833, 865, 3163, 3195
BpmI CTGGAG 2 cut(s) 2181, 2760
Bpu1102I GCTNAGC 1 cut(s) 3164
BsaAI YACGTR 1 cut(s) 2996
BsaBI GATNNNNATC 1 cut(s) 607
BsaJI CCNNGG 6 cut(s) 271, 410, 1034, 2405, 2930, 2944
BsaWI WCCGGW 2 cut(s) 634, 2584
BsaXI ACNNNNNCTCC 6 cut(s) 1317, 1347, 2288, 2318, 2553, 2583
Bsc4I CCNNNNNNNGG 3 cut(s) 272, 637, 1472
Bse1I ACTGG 8 cut(s) 12, 287, 860, 1799, 1800, 1895, 2164, 2480
Bse3DI GCAATG 2 cut(s) 573, 1506
Bse8I GATNNNNATC 1 cut(s) 607
BseBI CCWGG 7 cut(s) 273, 412, 420, 932, 957, 2469, 3003
BseDI CCNNGG 6 cut(s) 271, 410, 1034, 2405, 2930, 2944
BseGI GGATG 7 cut(s) 577, 1213, 2077, 2139, 2248, 2265, 2530
BseJI GATNNNNATC 1 cut(s) 607
BseLI CCNNNNNNNGG 3 cut(s) 272, 637, 1472
BseMI GCAATG 2 cut(s) 573, 1506
BseNI ACTGG 8 cut(s) 12, 287, 860, 1799, 1800, 1895, 2164, 2480
BsePI GCGCGC 1 cut(s) 1691
BseRI GAGGAG 2 cut(s) 203, 2361
BseXI GCAGC 7 cut(s) 1008, 1566, 2006, 2595, 2617, 2636, 3088
BsgI GTGCAG 2 cut(s) 1007, 2643
Bsh1236I CGCG 1 cut(s) 1693
BshFI GGCC 1 cut(s) 1450
BsiHKAI GWGCWC 1 cut(s) 2043
BsiSI CCGG 2 cut(s) 635, 2585
BslFI GGGAC 4 cut(s) 667, 2356, 2777, 3045
BslI CCNNNNNNNGG 3 cut(s) 272, 637, 1472
BsmAI GTCTC 4 cut(s) 980, 1320, 2480, 3166
BsmBI CGTCTC 1 cut(s) 980
BsmFI GGGAC 4 cut(s) 667, 2356, 2777, 3045
BsmI GAATGC 4 cut(s) 94, 478, 1063, 2827
BsnI GGCC 1 cut(s) 1450
Bsp1286I GDGCHC 2 cut(s) 1982, 2043
Bsp1407I TGTACA 1 cut(s) 1519
Bsp143I GATC 9 cut(s) 155, 223, 422, 817, 1372, 1543, 1882, 2108, 2400
Bsp1720I GCTNAGC 1 cut(s) 3164
BspACI CCGC 2 cut(s) 725, 1012
BspANI GGCC 1 cut(s) 1450
BspFNI CGCG 1 cut(s) 1693
BspHI TCATGA 3 cut(s) 508, 1591, 3127
BspLI GGNNCC 4 cut(s) 342, 2007, 2344, 2370
BspMAI CTGCAG 1 cut(s) 2635
BspOI GCTAGC 2 cut(s) 1691, 2327
BspPI GGATC 5 cut(s) 430, 1538, 1890, 2116, 2395
BspQI GCTCTTC 3 cut(s) 288, 726, 1833
BsrDI GCAATG 2 cut(s) 573, 1506
BsrGI TGTACA 1 cut(s) 1519
BsrI ACTGG 8 cut(s) 12, 287, 860, 1799, 1800, 1895, 2164, 2480
BssECI CCNNGG 6 cut(s) 271, 410, 1034, 2405, 2930, 2944
BssHII GCGCGC 1 cut(s) 1691
BssMI GATC 9 cut(s) 155, 223, 422, 817, 1372, 1543, 1882, 2108, 2400
BssNAI GTATAC 2 cut(s) 953, 2900
BssT1I CCWWGG 2 cut(s) 2405, 2944
Bst1107I GTATAC 2 cut(s) 953, 2900
Bst2UI CCWGG 7 cut(s) 273, 412, 420, 932, 957, 2469, 3003
Bst6I CTCTTC 7 cut(s) 288, 323, 726, 1616, 1646, 1833, 2693
BstAPI GCANNNNNTGC 2 cut(s) 2630, 3098
BstAUI TGTACA 1 cut(s) 1519
BstBAI YACGTR 1 cut(s) 2996
BstDSI CCRYGG 1 cut(s) 2930
BstENI CCTNNNNNAGG 1 cut(s) 1470
BstF5I GGATG 7 cut(s) 577, 1213, 2077, 2139, 2248, 2265, 2530
BstFNI CGCG 1 cut(s) 1693
BstHHI GCGC 2 cut(s) 1693, 1695
BstKTI GATC 9 cut(s) 158, 226, 425, 820, 1375, 1546, 1885, 2111, 2403
BstMAI GTCTC 4 cut(s) 980, 1320, 2480, 3166
BstMBI GATC 9 cut(s) 155, 223, 422, 817, 1372, 1543, 1882, 2108, 2400
BstNI CCWGG 7 cut(s) 273, 412, 420, 932, 957, 2469, 3003
BstNSI RCATGY 4 cut(s) 28, 167, 1965, 2323
BstSCI CCNGG 7 cut(s) 271, 410, 418, 930, 955, 2467, 3001
BstSFI CTRYAG 4 cut(s) 1215, 1944, 2631, 2668
BstUI CGCG 1 cut(s) 1693
BstV1I GCAGC 7 cut(s) 1008, 1566, 2006, 2595, 2617, 2636, 3088
BstV2I GAAGAC 4 cut(s) 497, 1238, 1580, 1797
BstX2I RGATCY 2 cut(s) 422, 1882
BstXI CCANNNNNNTGG 1 cut(s) 832
BstYI RGATCY 2 cut(s) 422, 1882
BstZ17I GTATAC 2 cut(s) 953, 2900
BsuI GTATCC 3 cut(s) 786, 839, 2592
BsuRI GGCC 1 cut(s) 1450
BtgI CCRYGG 1 cut(s) 2930
BtsCI GGATG 7 cut(s) 577, 1213, 2077, 2139, 2248, 2265, 2530
BtsI GCAGTG 2 cut(s) 1857, 3146
BtsIMutI CAGTG 7 cut(s) 5, 438, 1857, 2157, 2487, 2720, 3146
CaiI CAGNNNCTG 3 cut(s) 866, 2630, 3181
CciI TCATGA 3 cut(s) 508, 1591, 3127
CfoI GCGC 2 cut(s) 1693, 1695
Cfr13I GGNCC 6 cut(s) 1038, 1797, 2343, 2941, 2983, 2999
CseI GACGC 2 cut(s) 962, 2419
CsiI ACCWGGT 1 cut(s) 3001
Csp6I GTAC 6 cut(s) 117, 1087, 1137, 1269, 1520, 1722
CviQI GTAC 6 cut(s) 117, 1087, 1137, 1269, 1520, 1722
DpnI GATC 9 cut(s) 157, 225, 424, 819, 1374, 1545, 1884, 2110, 2402
DpnII GATC 9 cut(s) 155, 223, 422, 817, 1372, 1543, 1882, 2108, 2400
DraI TTTAAA 1 cut(s) 559
Eam1104I CTCTTC 7 cut(s) 288, 323, 726, 1616, 1646, 1833, 2693
EarI CTCTTC 7 cut(s) 288, 323, 726, 1616, 1646, 1833, 2693
Eco130I CCWWGG 2 cut(s) 2405, 2944
Eco24I GRGCYC 1 cut(s) 1982
Eco32I GATATC 4 cut(s) 83, 1504, 1988, 2169
Eco47I GGWCC 6 cut(s) 1038, 1797, 2343, 2941, 2983, 2999
Eco57I CTGAAG 4 cut(s) 723, 1271, 1667, 2957
EcoNI CCTNNNNNAGG 1 cut(s) 1470
EcoO109I RGGNCCY 2 cut(s) 2343, 2941
EcoRI GAATTC 1 cut(s) 405
EcoRII CCWGG 7 cut(s) 271, 410, 418, 930, 955, 2467, 3001
EcoRV GATATC 4 cut(s) 83, 1504, 1988, 2169
EcoT14I CCWWGG 2 cut(s) 2405, 2944
EcoT22I ATGCAT 4 cut(s) 94, 1967, 2530, 2803
EcoT38I GRGCYC 1 cut(s) 1982
ErhI CCWWGG 2 cut(s) 2405, 2944
Esp3I CGTCTC 1 cut(s) 980
FalI AAGNNNNNCTT 6 cut(s) 1765, 1797, 3081, 3113, 3147, 3179
FaqI GGGAC 4 cut(s) 667, 2356, 2777, 3045
FauNDI CATATG 3 cut(s) 1834, 2364, 2530
FbaI TGATCA 1 cut(s) 817
FblI GTMKAC 2 cut(s) 952, 2899
Fnu4HI GCNGC 7 cut(s) 1022, 1580, 1995, 2606, 2609, 2625, 3102
FokI GGATG 7 cut(s) 584, 1220, 2084, 2146, 2252, 2255, 2537
FriOI GRGCYC 1 cut(s) 1982
Fsp4HI GCNGC 7 cut(s) 1022, 1580, 1995, 2606, 2609, 2625, 3102
FspBI CTAG 6 cut(s) 986, 1688, 1958, 2190, 2324, 2576
GlaI GCGC 2 cut(s) 1692, 1694
GluI GCNGC 7 cut(s) 1022, 1580, 1995, 2606, 2609, 2625, 3102
GsuI CTGGAG 2 cut(s) 2181, 2760
HaeIII GGCC 1 cut(s) 1450
HapII CCGG 2 cut(s) 635, 2585
HgaI GACGC 2 cut(s) 962, 2419
HhaI GCGC 2 cut(s) 1693, 1695
Hin6I GCGC 2 cut(s) 1691, 1693
HinP1I GCGC 2 cut(s) 1691, 1693
HincII GTYRAC 2 cut(s) 810, 1853
HindII GTYRAC 2 cut(s) 810, 1853
HpaI GTTAAC 1 cut(s) 810
HpaII CCGG 2 cut(s) 635, 2585
HphI GGTGA 6 cut(s) 635, 1363, 1604, 1934, 2366, 3131
Hpy166II GTNNAC 8 cut(s) 810, 825, 953, 1271, 1724, 1853, 2900, 2999
Hpy8I GTNNAC 8 cut(s) 810, 825, 953, 1271, 1724, 1853, 2900, 2999
HpyCH4IV ACGT 3 cut(s) 1273, 1491, 2995
HpySE526I ACGT 3 cut(s) 1273, 1491, 2995
HspAI GCGC 2 cut(s) 1691, 1693
Ksp22I TGATCA 1 cut(s) 817
KspAI GTTAAC 1 cut(s) 810
Kzo9I GATC 9 cut(s) 155, 223, 422, 817, 1372, 1543, 1882, 2108, 2400
LguI GCTCTTC 3 cut(s) 288, 726, 1833
LmnI GCTCC 3 cut(s) 346, 2374, 3136
Lsp1109I GCAGC 7 cut(s) 1008, 1566, 2006, 2595, 2617, 2636, 3088
MabI ACCWGGT 1 cut(s) 3001
MaeI CTAG 6 cut(s) 986, 1688, 1958, 2190, 2324, 2576
MaeII ACGT 3 cut(s) 1273, 1491, 2995
MaeIII GTNAC 3 cut(s) 2127, 2847, 3005
MalI GATC 9 cut(s) 157, 225, 424, 819, 1374, 1545, 1884, 2110, 2402
MboI GATC 9 cut(s) 155, 223, 422, 817, 1372, 1543, 1882, 2108, 2400
MfeI CAATTG 1 cut(s) 1149
MflI RGATCY 2 cut(s) 422, 1882
MhlI GDGCHC 2 cut(s) 1982, 2043
MmeI TCCRAC 2 cut(s) 271, 2851
Mph1103I ATGCAT 4 cut(s) 94, 1967, 2530, 2803
MroXI GAANNNNTTC 1 cut(s) 708
MslI CAYNNNNRTG 2 cut(s) 440, 2511
MspA1I CMGCKG 3 cut(s) 20, 2608, 2673
MspI CCGG 2 cut(s) 635, 2585
MspR9I CCNGG 7 cut(s) 273, 412, 420, 932, 957, 2469, 3003
MunI CAATTG 1 cut(s) 1149
Mva1269I GAATGC 4 cut(s) 94, 478, 1063, 2827
MvaI CCWGG 7 cut(s) 273, 412, 420, 932, 957, 2469, 3003
MvnI CGCG 1 cut(s) 1693
NdeI CATATG 3 cut(s) 1834, 2364, 2530
NdeII GATC 9 cut(s) 155, 223, 422, 817, 1372, 1543, 1882, 2108, 2400
NheI GCTAGC 2 cut(s) 1687, 2323
NlaIV GGNNCC 4 cut(s) 342, 2007, 2344, 2370
NmuCI GTSAC 1 cut(s) 2127
NsiI ATGCAT 4 cut(s) 94, 1967, 2530, 2803
NspI RCATGY 4 cut(s) 28, 167, 1965, 2323
PaeI GCATGC 2 cut(s) 167, 1965
PagI TCATGA 3 cut(s) 508, 1591, 3127
PauI GCGCGC 1 cut(s) 1691
PciSI GCTCTTC 3 cut(s) 288, 726, 1833
PcsI WCGNNNNNNNCGW 2 cut(s) 1270, 2872
PctI GAATGC 4 cut(s) 94, 478, 1063, 2827
PdmI GAANNNNTTC 1 cut(s) 708
PflMI CCANNNNNTGG 1 cut(s) 272
PfoI TCCNGGA 2 cut(s) 418, 930
PkrI GCNGC 7 cut(s) 1023, 1581, 1996, 2607, 2610, 2626, 3103
Ppu21I YACGTR 1 cut(s) 2996
PpuMI RGGWCCY 2 cut(s) 2343, 2941
Psp5II RGGWCCY 2 cut(s) 2343, 2941
Psp6I CCWGG 7 cut(s) 271, 410, 418, 930, 955, 2467, 3001
PspGI CCWGG 7 cut(s) 271, 410, 418, 930, 955, 2467, 3001
PspN4I GGNNCC 4 cut(s) 342, 2007, 2344, 2370
PspPI GGNCC 6 cut(s) 1038, 1797, 2343, 2941, 2983, 2999
PspPPI RGGWCCY 2 cut(s) 2343, 2941
PstI CTGCAG 1 cut(s) 2635
PstNI CAGNNNCTG 3 cut(s) 866, 2630, 3181
PsuI RGATCY 2 cut(s) 422, 1882
PteI GCGCGC 1 cut(s) 1691
PvuII CAGCTG 3 cut(s) 20, 2608, 2673
RsaI GTAC 6 cut(s) 118, 1088, 1138, 1270, 1521, 1723
RsaNI GTAC 6 cut(s) 117, 1087, 1137, 1269, 1520, 1722
RseI CAYNNNNRTG 2 cut(s) 440, 2511
SapI GCTCTTC 3 cut(s) 288, 726, 1833
SatI GCNGC 7 cut(s) 1022, 1580, 1995, 2606, 2609, 2625, 3102
Sau3AI GATC 9 cut(s) 155, 223, 422, 817, 1372, 1543, 1882, 2108, 2400
Sau96I GGNCC 6 cut(s) 1038, 1797, 2343, 2941, 2983, 2999
ScaI AGTACT 2 cut(s) 118, 1088
ScrFI CCNGG 7 cut(s) 273, 412, 420, 932, 957, 2469, 3003
SduI GDGCHC 2 cut(s) 1982, 2043
SexAI ACCWGGT 1 cut(s) 3001
SfcI CTRYAG 4 cut(s) 1215, 1944, 2631, 2668
SinI GGWCC 6 cut(s) 1038, 1797, 2343, 2941, 2983, 2999
SmiMI CAYNNNNRTG 2 cut(s) 440, 2511
SpeI ACTAGT 1 cut(s) 2189
SphI GCATGC 2 cut(s) 167, 1965
SsiI CCGC 2 cut(s) 725, 1012
SspI AATATT 3 cut(s) 1399, 1540, 1662
SspMI CTAG 6 cut(s) 986, 1688, 1958, 2190, 2324, 2576
StyD4I CCNGG 7 cut(s) 271, 410, 418, 930, 955, 2467, 3001
StyI CCWWGG 2 cut(s) 2405, 2944
TaiI ACGT 3 cut(s) 1276, 1494, 2998
TaqI TCGA 2 cut(s) 154, 2544
TaqII GACCGA 1 cut(s) 216
TatI WGTACW 3 cut(s) 116, 1086, 1519
TscAI CASTG 7 cut(s) 12, 445, 1864, 2164, 2487, 2727, 3153
TseFI GTSAC 1 cut(s) 2127
TseI GCWGC 7 cut(s) 1021, 1579, 1994, 2605, 2608, 2624, 3101
Tsp45I GTSAC 1 cut(s) 2127
TspGWI ACGGA 2 cut(s) 2146, 2242
TspRI CASTG 7 cut(s) 12, 445, 1864, 2164, 2487, 2727, 3153
Van91I CCANNNNNTGG 1 cut(s) 272
VpaK11BI GGWCC 6 cut(s) 1038, 1797, 2343, 2941, 2983, 2999
XagI CCTNNNNNAGG 1 cut(s) 1470
XapI RAATTY 7 cut(s) 295, 405, 553, 1256, 1571, 2050, 3111
XceI RCATGY 4 cut(s) 28, 167, 1965, 2323
XmiI GTMKAC 2 cut(s) 952, 2899
XmnI GAANNNNTTC 1 cut(s) 708
XspI CTAG 6 cut(s) 986, 1688, 1958, 2190, 2324, 2576
ZrmI AGTACT 2 cut(s) 118, 1088
Zsp2I ATGCAT 4 cut(s) 94, 1967, 2530, 2803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.