Rh4BG398500

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0005436
Physical Location & Seq
Reverse (-)
54935429 .. 54944821
9393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG398500.1

Sequence Viewer

Length: 2967 bp
ATGGCACTGGCAGTTTCAGCTGGCATGTGTTTCGGTGGAAATGGATTGAGAAGTGCAATAGCTTTTGAAGCTGAGAAGGGATATCGGAATGCATTGGGTAGCAAAATGAGGTTCAGTACTTTTCTCCTTTCCAAAGTATTGAAATTATGTTCTCGATCAAAGCATAACTTTGCTAAGAGTATTTTGGAGGAGGCAGATAGTTATGGCATAGCAAGCATCTCAGATCGGTCAAAGTTGCTTAACAAGGTATCTGTTTTGATGGGTTATGATGGTCTTCATGATTTAATTGAGAATGAAAGAGCTGAAAAGCATTATGGTATGAATTTTAAAGATGCAATGGATGATTTTGATGCCTCCTTTGTCTGCAAGAGATTTCCATCAATCATTTTGGGTGATTCCCCTCCGGTGGAATTGTATGATGGGACTGCTAATTTCTTTGAAAAAATGACACTTTTAACAACTCAAAGTCCTGAAGGATTTCCTTCTGGCTCTACGGTTGAAGAGCAGGAGGGAGATTATCTCTATGAAACAGGGGATTCTCTGTATCCTGCGTTTCCAAGTGAAGAAACTTCCTTGTTAACAGATGATCAGTCCACGACTTTGGCTACTGAGAGATACTCATATCAACCAGTTCCTGTGGAGGAATCTTCTAACAAAGTTTCGCATAAATCACAAAAGAATGTGCCTGATGAGGTGTTCCTGGACAATTCTATCAGTTGTATACCTGGAATAAGTAAGAAGCGTCTCAGTCAGCTAGAAAATTGTGGCTTTCATACGGTGCGGAAATTGCTGCACCACTTTCCTCGGACCTATGCTGATTTACAGAATGCTCAAATTGACATAGATGATGGACAGTACTTGATTTTCATTGGAAAGATATTAAAATCAAGGGGAATCAAAGCTGGTACTTCTTTTTCAATTGTTGAGGTTGTCGTGGGTTGTGAAATTGCTGACAATAAAATAACCGAGCATAAGGATGATTCTACCGATTGTCGAAGGAAGACAATATATTTGCATCTGAAGAAATTTTTTCGTGGTACACGTTTTACTTCTCTACCGTTTCTTAGAATAGTTGAGAAGAAGCACAGAGAGGGAGACTTTGTATGTGTTAGTGGTAAGGTGAGGATAATGCCTGCCAAAGATCACTATGAAATGAGAGAATATAATATTGATGTCCTTAAAGATGAAAATGACTTGTCTTTTCATGCAAAAGGGAGGCCATATCCTATATACCCTTCAAAAGGAGGCTTAAATCCTGACGTTTTGAGAGATATCATTGCAAGAGTTGTACAAGTTTTACCTATCAATATTGATCCTATTCCTAAAAGAATCACTCTGGAATTTGGGCTGCTCAGTCTTCATGATGCTTATACTGAGATTCACCAACCGAAGAGTATGAGTGAAGCTGATTTGGCTCGGAAGAGGCTAATATTTGATGAGTTCTTTTACCTTCAGCTAGCGCGCTTATACCAAATGCTTGAAGGGCTTGGTACACAAATAGAGAAAGATGGATTGCTAAATAAGTATAGGAAACCTGAATCAAGTGCTGCTTATATGGAAGACTGGTCCAGTCTTACCAAGAAGTTTCTAAAAGCTCTTCCATATGCCCTTACGGGTAGTCAGCTCACTGCTGTTTCAGAAATTATCTGGGATCTCAGGCAACCAGTTCCAATGAATCGGCTTTTGCAGGGTGATGTTGGCTGTGGTAAAACTGTAGTTGCTTTTCTAGCATGCATGGAGGTAATGGGCTCTGGATACCAGGCAGCTTTCATGGTTCCTACTGAGTTGCTTGCCGTCCAGCATTACGAGCACTTGAAGAATTTGCTTGAAACTATGGAGGATGTTGAGTGCAAACCTTCTATTGCCTTACTAACAGGATCAACCCCATCAAAACAATCACGGATGATTCAAAAGAGTCTCCAGACTGGAGAGATATCAATGGTCATTGGAACGACTAGTTTAATAGCTGACCGTGTGGAATTCTCTGCATTACGGATTGCTGTGGTGGATGAACAACATCGTTTTGGTGTCATCCAGAGAGGACGGTTTAACAGTAAGCTATACTATACCTCCATAATATCTAACATGCTAGCCACAAACTCAGATGGGACCTCCAAAGGTGAAAAGCATATGGCTCCTCATGTTCTTGCCATGTCAGCTACTCCGATCCCAAGGACGCTTGCTCTGGCTTTATATGGGGATATGTCATTAACACAAATAACAGACTTGCCACCAGGAAGAATACCAGTGGAGACATTCATTATTCAAGGAAATGAAAATGGGTATGAGGATGCATATGAGATGATGCTTGATGAGTTAAAAGAAGGAGGCAAAGTGTATCTAGTGTATCCAGTCATTGAACAATCTGAGCAGCTGCCTCAACTCCGTGCAGCTTCTGCGGATTTTGAAGCTATATCTCATAGGTTTCAGGGCTACAGCTGTGGATTGTTGCATGGAAAAATGAAGAGTGATGAGAAAGATGAAGCACTGAGAAAGTTTAGGTCTGGAGAAACAAATATACTTCTTGCCACACAAGTAATTGAGATTGGTGTTGATGTCCCAGATGCATCTATGATGGTTGTTATGAATGCTGAGAGGTTTGGGATTGCTCAGTTACATCAGCTTAGAGGACGAGTTGGACGAGGTGTGAGAAAGTCTAAGTGTATACTGTTAGCATCTTCTGAGAGTAGCCTACCACGGCTGAAGGTCCTTGGGAAATCATCAGATGGGTTTTACTTGGCGAATATGGACCTTCTTTTACGTGGACCTGGTAACTTGCTTGGCAAGAAACAGTCGGGACATCTTCCAGAGTTTCCTATTGCAAGATTGGAAATAGATGGCAATATCTTACAAGAAGCACACCTTGCTGCTCTGAAAATTTTAGGTGATTCTCATGATTTGGAGCAATTCCCGCTGCTCAAAGCAGAGCTTAGCATGAGACAGCCTCTGTCTATTCTGGGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

988

Amino Acids

110.29

Weight (kDa)

6.19

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 540 - 722 3.4e-07 Type III restriction enzyme, res subunit
DEAD PF00270 559 - 726 3.6e-19 DEAD/DEAH box helicase
Helicase_C PF00271 768 - 881 9.6e-20 Helicase conserved C-terminal domain
RecG_dom3_C PF19833 908 - 964 4.1e-08 ATP-dependent DNA helicase RecG, domain 3, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 721, 2665
AccII CGCG 1 cut(s) 1462
AciI CCGC 3 cut(s) 781, 2399, 2912
AclWI GGATC 4 cut(s) 1307, 1659, 1885, 2161
AcsI RAATTY 6 cut(s) 322, 1025, 1340, 1819, 1979, 2877
AcuI CTGAAG 4 cut(s) 492, 1040, 1436, 2723
AfaI GTAC 6 cut(s) 118, 857, 907, 1039, 1290, 1492
AfiI CCNNNNNNNGG 2 cut(s) 406, 1241
AflIII ACRYGT 1 cut(s) 1040
AhlI ACTAGT 1 cut(s) 1955
AjnI CCWGG 5 cut(s) 699, 724, 1758, 2233, 2767
Alw21I GWGCWC 1 cut(s) 1812
Alw26I GTCTC 5 cut(s) 749, 1089, 1922, 2246, 2932
AlwI GGATC 4 cut(s) 1307, 1659, 1885, 2161
AlwNI CAGNNNCTG 3 cut(s) 635, 2396, 2947
AoxI GGCC 1 cut(s) 1217
ApeKI GCWGC 9 cut(s) 790, 1348, 1547, 1763, 2371, 2374, 2390, 2867, 2914
ApoI RAATTY 6 cut(s) 322, 1025, 1340, 1819, 1979, 2877
ArsI GACNNNNNNTTYG 2 cut(s) 994, 1026
Asp700I GAANNNNTTC 1 cut(s) 477
AspLEI GCGC 2 cut(s) 1462, 1464
AspS9I GGNCC 6 cut(s) 807, 1566, 2109, 2707, 2749, 2765
AsuHPI GGTGA 6 cut(s) 404, 1132, 1373, 1703, 2132, 2897
AsuNHI GCTAGC 2 cut(s) 1456, 2089
AvaII GGWCC 6 cut(s) 807, 1566, 2109, 2707, 2749, 2765
BanII GRGCYC 1 cut(s) 1751
BbsI GAAGAC 4 cut(s) 266, 1007, 1349, 1566
Bbv12I GWGCWC 1 cut(s) 1812
BbvI GCAGC 9 cut(s) 777, 1335, 1534, 1775, 2361, 2383, 2402, 2854, 2901
BceAI ACGGC 2 cut(s) 1778, 2714
BcgI CGANNNNNNTGC 2 cut(s) 13, 47
BciT130I CCWGG 5 cut(s) 701, 726, 1760, 2235, 2769
BciVI GTATCC 3 cut(s) 555, 1748, 2358
BclI TGATCA 1 cut(s) 586
BcoDI GTCTC 5 cut(s) 749, 1089, 1922, 2246, 2932
BcuI ACTAGT 1 cut(s) 1955
BfaI CTAG 6 cut(s) 755, 1457, 1727, 1956, 2090, 2342
BfmI CTRYAG 2 cut(s) 1713, 2434
BfuI GTATCC 3 cut(s) 555, 1748, 2358
BisI GCNGC 9 cut(s) 791, 1349, 1548, 1764, 2372, 2375, 2391, 2868, 2915
BlpI GCTNAGC 1 cut(s) 2930
BlsI GCNGC 9 cut(s) 792, 1350, 1549, 1765, 2373, 2376, 2392, 2869, 2916
BmcAI AGTACT 2 cut(s) 118, 857
Bme1390I CCNGG 5 cut(s) 701, 726, 1760, 2235, 2769
Bme18I GGWCC 6 cut(s) 807, 1566, 2109, 2707, 2749, 2765
BmgT120I GGNCC 6 cut(s) 807, 1566, 2109, 2707, 2749, 2765
BmiI GGNNCC 3 cut(s) 1776, 2110, 2136
BmrFI CCNGG 5 cut(s) 701, 726, 1760, 2235, 2769
BmtI GCTAGC 2 cut(s) 1460, 2093
BpiI GAAGAC 4 cut(s) 266, 1007, 1349, 1566
BplI GAGNNNNNCTC 6 cut(s) 504, 536, 602, 634, 2929, 2961
BpmI CTGGAG 3 cut(s) 1904, 1947, 2526
Bpu1102I GCTNAGC 1 cut(s) 2930
BsaAI YACGTR 1 cut(s) 2762
BsaBI GATNNNNATC 1 cut(s) 376
BsaJI CCNNGG 4 cut(s) 803, 2171, 2696, 2710
BsaWI WCCGGW 1 cut(s) 403
BsaXI ACNNNNNCTCC 6 cut(s) 1086, 1116, 2054, 2084, 2319, 2349
Bsc4I CCNNNNNNNGG 2 cut(s) 406, 1241
Bse1I ACTGG 8 cut(s) 12, 629, 1568, 1569, 1664, 1930, 2246, 2351
Bse3DI GCAATG 2 cut(s) 342, 1275
Bse8I GATNNNNATC 1 cut(s) 376
BseBI CCWGG 5 cut(s) 701, 726, 1760, 2235, 2769
BseDI CCNNGG 4 cut(s) 803, 2171, 2696, 2710
BseGI GGATG 7 cut(s) 346, 982, 1846, 1908, 2014, 2031, 2296
BseJI GATNNNNATC 1 cut(s) 376
BseLI CCNNNNNNNGG 2 cut(s) 406, 1241
BseMI GCAATG 2 cut(s) 342, 1275
BseNI ACTGG 8 cut(s) 12, 629, 1568, 1569, 1664, 1930, 2246, 2351
BsePI GCGCGC 1 cut(s) 1460
BseRI GAGGAG 2 cut(s) 203, 2127
BseXI GCAGC 9 cut(s) 777, 1335, 1534, 1775, 2361, 2383, 2402, 2854, 2901
BsgI GTGCAG 2 cut(s) 776, 2409
Bsh1236I CGCG 1 cut(s) 1462
BshFI GGCC 1 cut(s) 1219
BsiHKAI GWGCWC 1 cut(s) 1812
BsiSI CCGG 1 cut(s) 404
BslFI GGGAC 4 cut(s) 436, 2122, 2543, 2811
BslI CCNNNNNNNGG 2 cut(s) 406, 1241
BsmAI GTCTC 5 cut(s) 749, 1089, 1922, 2246, 2932
BsmBI CGTCTC 1 cut(s) 749
BsmFI GGGAC 4 cut(s) 436, 2122, 2543, 2811
BsmI GAATGC 3 cut(s) 94, 832, 2593
BsnI GGCC 1 cut(s) 1219
Bsp1286I GDGCHC 2 cut(s) 1751, 1812
Bsp1407I TGTACA 1 cut(s) 1288
Bsp143I GATC 8 cut(s) 155, 223, 586, 1141, 1312, 1651, 1877, 2166
Bsp1720I GCTNAGC 1 cut(s) 2930
BspACI CCGC 3 cut(s) 781, 2399, 2912
BspANI GGCC 1 cut(s) 1219
BspFNI CGCG 1 cut(s) 1462
BspHI TCATGA 3 cut(s) 277, 1360, 2893
BspLI GGNNCC 3 cut(s) 1776, 2110, 2136
BspOI GCTAGC 2 cut(s) 1460, 2093
BspPI GGATC 4 cut(s) 1307, 1659, 1885, 2161
BspQI GCTCTTC 2 cut(s) 495, 1602
BsrDI GCAATG 2 cut(s) 342, 1275
BsrGI TGTACA 1 cut(s) 1288
BsrI ACTGG 8 cut(s) 12, 629, 1568, 1569, 1664, 1930, 2246, 2351
BssECI CCNNGG 4 cut(s) 803, 2171, 2696, 2710
BssHII GCGCGC 1 cut(s) 1460
BssMI GATC 8 cut(s) 155, 223, 586, 1141, 1312, 1651, 1877, 2166
BssNAI GTATAC 2 cut(s) 722, 2666
BssT1I CCWWGG 2 cut(s) 2171, 2710
Bst1107I GTATAC 2 cut(s) 722, 2666
Bst2UI CCWGG 5 cut(s) 701, 726, 1760, 2235, 2769
Bst6I CTCTTC 5 cut(s) 495, 1385, 1415, 1602, 2459
BstAPI GCANNNNNTGC 2 cut(s) 2396, 2864
BstAUI TGTACA 1 cut(s) 1288
BstBAI YACGTR 1 cut(s) 2762
BstC8I GCNNGC 9 cut(s) 22, 214, 1134, 1458, 1462, 1732, 1791, 2091, 2181
BstDSI CCRYGG 1 cut(s) 2696
BstENI CCTNNNNNAGG 1 cut(s) 1239
BstF5I GGATG 7 cut(s) 346, 982, 1846, 1908, 2014, 2031, 2296
BstFNI CGCG 1 cut(s) 1462
BstHHI GCGC 2 cut(s) 1462, 1464
BstKTI GATC 8 cut(s) 158, 226, 589, 1144, 1315, 1654, 1880, 2169
BstMAI GTCTC 5 cut(s) 749, 1089, 1922, 2246, 2932
BstMBI GATC 8 cut(s) 155, 223, 586, 1141, 1312, 1651, 1877, 2166
BstNI CCWGG 5 cut(s) 701, 726, 1760, 2235, 2769
BstNSI RCATGY 3 cut(s) 28, 1734, 2089
BstSCI CCNGG 5 cut(s) 699, 724, 1758, 2233, 2767
BstSFI CTRYAG 2 cut(s) 1713, 2434
BstUI CGCG 1 cut(s) 1462
BstV1I GCAGC 9 cut(s) 777, 1335, 1534, 1775, 2361, 2383, 2402, 2854, 2901
BstV2I GAAGAC 4 cut(s) 266, 1007, 1349, 1566
BstX2I RGATCY 1 cut(s) 1651
BstXI CCANNNNNNTGG 1 cut(s) 601
BstYI RGATCY 1 cut(s) 1651
BstZ17I GTATAC 2 cut(s) 722, 2666
BsuI GTATCC 3 cut(s) 555, 1748, 2358
BsuRI GGCC 1 cut(s) 1219
BtgI CCRYGG 1 cut(s) 2696
BtsCI GGATG 7 cut(s) 346, 982, 1846, 1908, 2014, 2031, 2296
BtsI GCAGTG 1 cut(s) 1626
BtsIMutI CAGTG 4 cut(s) 5, 1626, 2253, 2486
Cac8I GCNNGC 9 cut(s) 22, 214, 1134, 1458, 1462, 1732, 1791, 2091, 2181
CaiI CAGNNNCTG 3 cut(s) 635, 2396, 2947
CciI TCATGA 3 cut(s) 277, 1360, 2893
CfoI GCGC 2 cut(s) 1462, 1464
Cfr13I GGNCC 6 cut(s) 807, 1566, 2109, 2707, 2749, 2765
CseI GACGC 2 cut(s) 731, 2185
CsiI ACCWGGT 1 cut(s) 2767
Csp6I GTAC 6 cut(s) 117, 856, 906, 1038, 1289, 1491
CviQI GTAC 6 cut(s) 117, 856, 906, 1038, 1289, 1491
DpnI GATC 8 cut(s) 157, 225, 588, 1143, 1314, 1653, 1879, 2168
DpnII GATC 8 cut(s) 155, 223, 586, 1141, 1312, 1651, 1877, 2166
DraI TTTAAA 1 cut(s) 328
Eam1104I CTCTTC 5 cut(s) 495, 1385, 1415, 1602, 2459
EarI CTCTTC 5 cut(s) 495, 1385, 1415, 1602, 2459
Eco130I CCWWGG 2 cut(s) 2171, 2710
Eco24I GRGCYC 1 cut(s) 1751
Eco32I GATATC 3 cut(s) 83, 1273, 1935
Eco47I GGWCC 6 cut(s) 807, 1566, 2109, 2707, 2749, 2765
Eco57I CTGAAG 4 cut(s) 492, 1040, 1436, 2723
EcoNI CCTNNNNNAGG 1 cut(s) 1239
EcoO109I RGGNCCY 2 cut(s) 2109, 2707
EcoRI GAATTC 1 cut(s) 1979
EcoRII CCWGG 5 cut(s) 699, 724, 1758, 2233, 2767
EcoRV GATATC 3 cut(s) 83, 1273, 1935
EcoT14I CCWWGG 2 cut(s) 2171, 2710
EcoT22I ATGCAT 4 cut(s) 94, 1736, 2296, 2569
EcoT38I GRGCYC 1 cut(s) 1751
ErhI CCWWGG 2 cut(s) 2171, 2710
Esp3I CGTCTC 1 cut(s) 749
FalI AAGNNNNNCTT 8 cut(s) 152, 184, 1534, 1566, 2847, 2879, 2913, 2945
FaqI GGGAC 4 cut(s) 436, 2122, 2543, 2811
FauI CCCGC 1 cut(s) 2919
FauNDI CATATG 3 cut(s) 1603, 2130, 2296
FbaI TGATCA 1 cut(s) 586
FblI GTMKAC 2 cut(s) 721, 2665
Fnu4HI GCNGC 9 cut(s) 791, 1349, 1548, 1764, 2372, 2375, 2391, 2868, 2915
FokI GGATG 7 cut(s) 353, 989, 1853, 1915, 2018, 2021, 2303
FriOI GRGCYC 1 cut(s) 1751
Fsp4HI GCNGC 9 cut(s) 791, 1349, 1548, 1764, 2372, 2375, 2391, 2868, 2915
FspBI CTAG 6 cut(s) 755, 1457, 1727, 1956, 2090, 2342
GlaI GCGC 2 cut(s) 1461, 1463
GluI GCNGC 9 cut(s) 791, 1349, 1548, 1764, 2372, 2375, 2391, 2868, 2915
GsuI CTGGAG 3 cut(s) 1904, 1947, 2526
HaeIII GGCC 1 cut(s) 1219
HapII CCGG 1 cut(s) 404
HgaI GACGC 2 cut(s) 731, 2185
HhaI GCGC 2 cut(s) 1462, 1464
Hin6I GCGC 2 cut(s) 1460, 1462
HinP1I GCGC 2 cut(s) 1460, 1462
HincII GTYRAC 1 cut(s) 579
HindII GTYRAC 1 cut(s) 579
HpaI GTTAAC 1 cut(s) 579
HpaII CCGG 1 cut(s) 404
HphI GGTGA 6 cut(s) 404, 1132, 1373, 1703, 2132, 2897
Hpy166II GTNNAC 7 cut(s) 579, 594, 722, 1040, 1493, 2666, 2765
Hpy8I GTNNAC 7 cut(s) 579, 594, 722, 1040, 1493, 2666, 2765
HpyCH4IV ACGT 3 cut(s) 1042, 1260, 2761
HpySE526I ACGT 3 cut(s) 1042, 1260, 2761
HspAI GCGC 2 cut(s) 1460, 1462
Ksp22I TGATCA 1 cut(s) 586
KspAI GTTAAC 1 cut(s) 579
Kzo9I GATC 8 cut(s) 155, 223, 586, 1141, 1312, 1651, 1877, 2166
LguI GCTCTTC 2 cut(s) 495, 1602
LmnI GCTCC 2 cut(s) 2140, 2902
Lsp1109I GCAGC 9 cut(s) 777, 1335, 1534, 1775, 2361, 2383, 2402, 2854, 2901
MabI ACCWGGT 1 cut(s) 2767
MaeI CTAG 6 cut(s) 755, 1457, 1727, 1956, 2090, 2342
MaeII ACGT 3 cut(s) 1042, 1260, 2761
MaeIII GTNAC 3 cut(s) 2613, 2771, 2960
MalI GATC 8 cut(s) 157, 225, 588, 1143, 1314, 1653, 1879, 2168
MboI GATC 8 cut(s) 155, 223, 586, 1141, 1312, 1651, 1877, 2166
MfeI CAATTG 1 cut(s) 918
MflI RGATCY 1 cut(s) 1651
MhlI GDGCHC 2 cut(s) 1751, 1812
MlyI GAGTC 1 cut(s) 1924
MmeI TCCRAC 1 cut(s) 2617
Mph1103I ATGCAT 4 cut(s) 94, 1736, 2296, 2569
MroXI GAANNNNTTC 1 cut(s) 477
MslI CAYNNNNRTG 1 cut(s) 975
MspA1I CMGCKG 4 cut(s) 20, 2374, 2439, 2914
MspI CCGG 1 cut(s) 404
MspR9I CCNGG 5 cut(s) 701, 726, 1760, 2235, 2769
MunI CAATTG 1 cut(s) 918
Mva1269I GAATGC 3 cut(s) 94, 832, 2593
MvaI CCWGG 5 cut(s) 701, 726, 1760, 2235, 2769
MvnI CGCG 1 cut(s) 1462
NdeI CATATG 3 cut(s) 1603, 2130, 2296
NdeII GATC 8 cut(s) 155, 223, 586, 1141, 1312, 1651, 1877, 2166
NheI GCTAGC 2 cut(s) 1456, 2089
NlaIV GGNNCC 3 cut(s) 1776, 2110, 2136
NmuCI GTSAC 1 cut(s) 2960
NsiI ATGCAT 4 cut(s) 94, 1736, 2296, 2569
NspI RCATGY 3 cut(s) 28, 1734, 2089
PaeI GCATGC 1 cut(s) 1734
PagI TCATGA 3 cut(s) 277, 1360, 2893
PauI GCGCGC 1 cut(s) 1460
PciSI GCTCTTC 2 cut(s) 495, 1602
PcsI WCGNNNNNNNCGW 2 cut(s) 1039, 2638
PctI GAATGC 3 cut(s) 94, 832, 2593
PdmI GAANNNNTTC 1 cut(s) 477
PfoI TCCNGGA 1 cut(s) 699
PkrI GCNGC 9 cut(s) 792, 1350, 1549, 1765, 2373, 2376, 2392, 2869, 2916
PleI GAGTC 1 cut(s) 1923
PpsI GAGTC 1 cut(s) 1923
Ppu21I YACGTR 1 cut(s) 2762
PpuMI RGGWCCY 2 cut(s) 2109, 2707
Psp5II RGGWCCY 2 cut(s) 2109, 2707
Psp6I CCWGG 5 cut(s) 699, 724, 1758, 2233, 2767
PspGI CCWGG 5 cut(s) 699, 724, 1758, 2233, 2767
PspN4I GGNNCC 3 cut(s) 1776, 2110, 2136
PspPI GGNCC 6 cut(s) 807, 1566, 2109, 2707, 2749, 2765
PspPPI RGGWCCY 2 cut(s) 2109, 2707
PstNI CAGNNNCTG 3 cut(s) 635, 2396, 2947
PsuI RGATCY 1 cut(s) 1651
PteI GCGCGC 1 cut(s) 1460
PvuII CAGCTG 3 cut(s) 20, 2374, 2439
RsaI GTAC 6 cut(s) 118, 857, 907, 1039, 1290, 1492
RsaNI GTAC 6 cut(s) 117, 856, 906, 1038, 1289, 1491
RseI CAYNNNNRTG 1 cut(s) 975
SapI GCTCTTC 2 cut(s) 495, 1602
SatI GCNGC 9 cut(s) 791, 1349, 1548, 1764, 2372, 2375, 2391, 2868, 2915
Sau3AI GATC 8 cut(s) 155, 223, 586, 1141, 1312, 1651, 1877, 2166
Sau96I GGNCC 6 cut(s) 807, 1566, 2109, 2707, 2749, 2765
ScaI AGTACT 2 cut(s) 118, 857
SchI GAGTC 1 cut(s) 1924
ScrFI CCNGG 5 cut(s) 701, 726, 1760, 2235, 2769
SduI GDGCHC 2 cut(s) 1751, 1812
SexAI ACCWGGT 1 cut(s) 2767
SfcI CTRYAG 2 cut(s) 1713, 2434
SinI GGWCC 6 cut(s) 807, 1566, 2109, 2707, 2749, 2765
SmiMI CAYNNNNRTG 1 cut(s) 975
SpeI ACTAGT 1 cut(s) 1955
SphI GCATGC 1 cut(s) 1734
SsiI CCGC 3 cut(s) 781, 2399, 2912
SspI AATATT 3 cut(s) 1168, 1309, 1431
SspMI CTAG 6 cut(s) 755, 1457, 1727, 1956, 2090, 2342
StyD4I CCNGG 5 cut(s) 699, 724, 1758, 2233, 2767
StyI CCWWGG 2 cut(s) 2171, 2710
TaiI ACGT 3 cut(s) 1045, 1263, 2764
TaqI TCGA 2 cut(s) 154, 994
TaqII GACCGA 1 cut(s) 216
TatI WGTACW 3 cut(s) 116, 855, 1288
TscAI CASTG 4 cut(s) 12, 1633, 2253, 2493
TseFI GTSAC 1 cut(s) 2960
TseI GCWGC 9 cut(s) 790, 1348, 1547, 1763, 2371, 2374, 2390, 2867, 2914
Tsp45I GTSAC 1 cut(s) 2960
TspGWI ACGGA 3 cut(s) 1915, 2008, 2375
TspRI CASTG 4 cut(s) 12, 1633, 2253, 2493
VpaK11BI GGWCC 6 cut(s) 807, 1566, 2109, 2707, 2749, 2765
XagI CCTNNNNNAGG 1 cut(s) 1239
XapI RAATTY 6 cut(s) 322, 1025, 1340, 1819, 1979, 2877
XceI RCATGY 3 cut(s) 28, 1734, 2089
XmiI GTMKAC 2 cut(s) 721, 2665
XmnI GAANNNNTTC 1 cut(s) 477
XspI CTAG 6 cut(s) 755, 1457, 1727, 1956, 2090, 2342
ZrmI AGTACT 2 cut(s) 118, 857
Zsp2I ATGCAT 4 cut(s) 94, 1736, 2296, 2569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.