Rw4G033180

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005436
Physical Location & Seq
Reverse (-)
60276709 .. 60287459
10751 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G033180.1

Sequence Viewer

Length: 2979 bp
ATGGCACTGGCAGTTTCAGCTGGCATGTGTTTTGGTGGAAATGGATTGAGAAGTGCAATAGCTTTTGAAGCTGAGAAGGGATATCGGAATGCATTGGGTAGCAAAATGAGTTTTTATTGCAGGTTCAGTACTTTTCTCCTTTCCAAAGTATTGAAATTATGTTCTCGATCAAAGCATACTTTTGCTAAGAGTATTTTGGAGGAGGCAGATAGTTATGGCATAGCAAGCATCTCAGATCGGTCAAAGTTGCTTAACAAGGTATCTGTTTTGATGGGTTATGATGGTCTTCATGATTTAATTGAGAATGAAAGAGCTGAAAAGCATTATGGTATGAGTTTTAAAGATGCAATGGATGATTTTGATGCCTCCTTTGTCTGCAAGAGATTTCCATCAATCATTTTGGGTGATTCCCCTCCGGTGGAATTGTATGATGGGACTGCTAATTTCTTTGAAAAAATGACACTTTTAACAACTCAAAGTCCTGAAGGATTTCCTTCTGGCTCTACGGTTGAAGAGCAGGAGGGTGATTATCTCTATGAAACAGGGGATTCTCTGTATCCTGCGTTTCCAAGTGAAGAAACTTCCTTGTTAACAGATGATCAGTCCACGACTTTGGCTACTGAGAGATACACATATCAACCAGTTCCTGTGGAGGAATCTTCTAACAAAGTTTCGCATAAATCACAAAAGAATGTGCCTGATGAGGTGTTCCTGGACAATTCTATCAGTTGTATACCTGGAATAAGTAAGAAGCGTCTCAATCAGCTAGAAAATTGTGGCTTTCATACGGTGCGGAAATTGCTGCACCACTTTCCTCGGACCTACGCTGATTTACAGAATGCTCAAATTGACATAGATGATGGCCAGTACTTGATTTTCATTGGAAAGATATTAAAATCAAGGGGAATCAAAGCTGGTACTTCTTTTTCAATTGTTGAGGTTGTTGTGGGTTGTGAAATTGCTGACGATAAAATAACCGAGCATAAGGATGATTCTACGGATTGTAGAAGGAAGACAATATATTTGCATCTGAAGAAATTTTTTCGTGGTACACGTTTTACCTCTCTACCGTTTCTTAGAATAGTTGAGAAGAAGCACAGAGAGGGAGACTTTGTATGTGTTAGTGGTAAGGTGAGGATAATGCCTGCTAAAGATCACTATGAAATGAGAGAATATAATATTGATGTCCTTAAAGATGAAAATGACTTGTCTTTTCATGCAAAAGGGAGGCCATATCCTATATACCCTTCAAAAGGAGGCTTAAATCCTGACGTTTTGAGAGATATCATTGCAAGAGTTGTACAAGTTTTACCTATCAATATTGATCCTATTCCTAAAAGAATCACTCTGGAATTTGGGCTGCTCAGTCTTCATGATGCTTATACTGAGATTCACCAACCAAAGAGTATGAGTGAAGCTGATTTGGCTCGGAAGAGGCTAATATTTGATGAGTTCTTTTACCTTCAGCTAGCGCGCTTATACCAAATGCTTGAAGGGCTTGGTACACAAATAGAGAAAGATGGATTGCTAAATAAGTATAGGAAACCTGAATCAAGTGCTGCTTATATGGAAGACTGGTCCAGTCTTACCAAGAAGTTTCTAAAAGCTCTTCCATATGCCCTTACGGGTAGTCAGCTGACTGCTGTTTCAGAAATTATCTGGGATCTCAGGCAACCAGTTCCAATGAATCGACTTTTGCAGGGTGATGTTGGCTGCGGTAAAACTGTTGTTGCTTTTCTAGCATGCATGGAGGTAATGGGCTCTGGATATCAGGCAGCTTTCATGGTTCCTACTGAGTTGCTTGCCGTCCAGCATTACGAGCACTTGAAGAATTTGCTTGAAACCATGGAGGATGTTGAGTGCAAACCTTCTATTGCCTTACTAACAGGATCAACCCCGTCAAAACAGTCACGGATGATTCAGAAGAGTCTCCAGACTGGAGAGATATCAATGGTCATTGGAACGACTAGTTTAATAGCTGACCGTGTGGAGTTCTCTGCATTACGGATTGCTGTGGTGGATGAACAACATCGTTTTGGTGTCATCCAGAGAGGACGGTTTAACAGTAAGCTATACTATACCTCCATAATATCAAACATGCTAGCCACAAACTCGGATGGAGCCTCCAAAGGTGAAAAGCATATGGCTCCTCATGTTCTTGCCATGTCAGCTACTCCGATCCCAAGGACGCTTGCTCTGGCTTTATATGGGGATATGTCATTAACACAAATAACAGACTTGCCACCAGGAAGAATACCAGTGGAGACATTCATTATTCAAGGAAATGAAAATGGGTATGAGGATGCATATGAGATGATGCTCGATGAGTTAAAAGAAGGAGGCAAAGTGTATCTAGTGTATCCAGTCATTGAACAATCTGAGCAGCTGCCTCAACTCCGTGCAGCTTCTGCGGATTTTGAAGCTATATCTCATAGGTTTCAGGGCTACAGCTGTGGATTGTTGCATGGAAAAATGAAGAGTGATGAGAAAGATGAAGCACTGAGAAAGTTTAGGTCTGGAGAAACAAATATACTTCTTGCCACACAAGTAATTGAGATTGGTGTTGATGTCCCAGATGCATCTATGATGGTTGTTATGAATGCTGAGAGGTTTGGGATTGCTCAGTTACATCAGCTTAGAGGACGAGTTGGACGAGGTGTGAGAAAGTCTAAGTGTATACTGTTAGCATCTTCTGAGAGTAGCCTACCACGGCTGAAGGTCCTTGGGAAATCATCAGATGGGTTTTACTTGGCGAATATGGACCTTCTTTTACGTGGACCTGGTAACTTGCTTGGCAAGAAACAGTCGGGACATCTTCCAGAGTTTCCTATTGCAAGATTGGAAATAGATGGCAATATCTTACAAGAAGCACACCTTGCTGCTCTGAAAATTTTAGGTGATTCTCATGATTTGGAGCAATTCCCGCTGCTCAAAGCAGAGCTTAGCATGAGACAGCCTCTTTCTATTCTGGGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

992

Amino Acids

110.76

Weight (kDa)

6.14

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 544 - 726 3.3e-07 Type III restriction enzyme, res subunit
DEAD PF00270 563 - 730 3.7e-19 DEAD/DEAH box helicase
Helicase_C PF00271 772 - 885 9.7e-20 Helicase conserved C-terminal domain
RecG_dom3_C PF19833 912 - 968 4.1e-08 ATP-dependent DNA helicase RecG, domain 3, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 111
AccI GTMKAC 2 cut(s) 733, 2677
AccII CGCG 1 cut(s) 1474
AciI CCGC 4 cut(s) 793, 1716, 2411, 2924
AclWI GGATC 4 cut(s) 1319, 1671, 1897, 2173
AcoI YGGCCR 1 cut(s) 862
AcsI RAATTY 4 cut(s) 1037, 1352, 1831, 2889
AcuI CTGAAG 4 cut(s) 504, 1052, 1448, 2735
AfaI GTAC 6 cut(s) 130, 869, 919, 1051, 1302, 1504
AfiI CCNNNNNNNGG 2 cut(s) 418, 1253
AflIII ACRYGT 1 cut(s) 1052
AhlI ACTAGT 1 cut(s) 1967
AjnI CCWGG 4 cut(s) 711, 736, 2245, 2779
Alw21I GWGCWC 1 cut(s) 1824
Alw26I GTCTC 5 cut(s) 761, 1101, 1934, 2258, 2944
AlwI GGATC 4 cut(s) 1319, 1671, 1897, 2173
AlwNI CAGNNNCTG 2 cut(s) 647, 2408
AoxI GGCC 2 cut(s) 862, 1229
ApoI RAATTY 4 cut(s) 1037, 1352, 1831, 2889
ArsI GACNNNNNNTTYG 2 cut(s) 1006, 1038
Asp700I GAANNNNTTC 1 cut(s) 489
AspLEI GCGC 2 cut(s) 1474, 1476
AspS9I GGNCC 5 cut(s) 819, 1578, 2719, 2761, 2777
AsuHPI GGTGA 7 cut(s) 416, 536, 1144, 1385, 1715, 2144, 2909
AsuNHI GCTAGC 2 cut(s) 1468, 2101
AvaII GGWCC 5 cut(s) 819, 1578, 2719, 2761, 2777
BalI TGGCCA 1 cut(s) 864
BanII GRGCYC 1 cut(s) 1763
BbsI GAAGAC 4 cut(s) 278, 1019, 1361, 1578
Bbv12I GWGCWC 1 cut(s) 1824
BceAI ACGGC 2 cut(s) 1790, 2726
BciT130I CCWGG 4 cut(s) 713, 738, 2247, 2781
BciVI GTATCC 2 cut(s) 567, 2370
BclI TGATCA 1 cut(s) 598
BcoDI GTCTC 5 cut(s) 761, 1101, 1934, 2258, 2944
BcuI ACTAGT 1 cut(s) 1967
BfaI CTAG 6 cut(s) 767, 1469, 1739, 1968, 2102, 2354
BfmI CTRYAG 1 cut(s) 2446
BfuAI ACCTGC 1 cut(s) 111
BfuI GTATCC 2 cut(s) 567, 2370
BlpI GCTNAGC 1 cut(s) 2942
BmcAI AGTACT 2 cut(s) 130, 869
Bme1390I CCNGG 4 cut(s) 713, 738, 2247, 2781
Bme18I GGWCC 5 cut(s) 819, 1578, 2719, 2761, 2777
BmgT120I GGNCC 5 cut(s) 819, 1578, 2719, 2761, 2777
BmiI GGNNCC 3 cut(s) 1788, 2122, 2148
BmrFI CCNGG 4 cut(s) 713, 738, 2247, 2781
BmtI GCTAGC 2 cut(s) 1472, 2105
BpiI GAAGAC 4 cut(s) 278, 1019, 1361, 1578
BplI GAGNNNNNCTC 2 cut(s) 2941, 2973
BpmI CTGGAG 3 cut(s) 1916, 1959, 2538
Bpu1102I GCTNAGC 1 cut(s) 2942
BsaAI YACGTR 1 cut(s) 2774
BsaBI GATNNNNATC 1 cut(s) 388
BsaJI CCNNGG 5 cut(s) 815, 1845, 2183, 2708, 2722
BsaWI WCCGGW 1 cut(s) 415
BsaXI ACNNNNNCTCC 6 cut(s) 1098, 1128, 2066, 2096, 2331, 2361
Bsc4I CCNNNNNNNGG 2 cut(s) 418, 1253
Bse1I ACTGG 9 cut(s) 12, 641, 865, 1580, 1581, 1676, 1942, 2258, 2363
Bse3DI GCAATG 2 cut(s) 354, 1287
Bse8I GATNNNNATC 1 cut(s) 388
BseBI CCWGG 4 cut(s) 713, 738, 2247, 2781
BseDI CCNNGG 5 cut(s) 815, 1845, 2183, 2708, 2722
BseGI GGATG 8 cut(s) 358, 994, 1858, 1920, 2026, 2043, 2122, 2308
BseJI GATNNNNATC 1 cut(s) 388
BseLI CCNNNNNNNGG 2 cut(s) 418, 1253
BseMI GCAATG 2 cut(s) 354, 1287
BseNI ACTGG 9 cut(s) 12, 641, 865, 1580, 1581, 1676, 1942, 2258, 2363
BsePI GCGCGC 1 cut(s) 1472
BseRI GAGGAG 2 cut(s) 215, 2139
BsgI GTGCAG 2 cut(s) 788, 2421
Bsh1236I CGCG 1 cut(s) 1474
BshFI GGCC 2 cut(s) 864, 1231
BsiHKAI GWGCWC 1 cut(s) 1824
BsiSI CCGG 1 cut(s) 416
BslFI GGGAC 3 cut(s) 448, 2555, 2823
BslI CCNNNNNNNGG 2 cut(s) 418, 1253
BsmAI GTCTC 5 cut(s) 761, 1101, 1934, 2258, 2944
BsmBI CGTCTC 1 cut(s) 761
BsmFI GGGAC 3 cut(s) 448, 2555, 2823
BsmI GAATGC 3 cut(s) 94, 844, 2605
BsnI GGCC 2 cut(s) 864, 1231
Bsp1286I GDGCHC 2 cut(s) 1763, 1824
Bsp1407I TGTACA 1 cut(s) 1300
Bsp143I GATC 8 cut(s) 167, 235, 598, 1153, 1324, 1663, 1889, 2178
Bsp1720I GCTNAGC 1 cut(s) 2942
Bsp19I CCATGG 1 cut(s) 1845
BspACI CCGC 4 cut(s) 793, 1716, 2411, 2924
BspANI GGCC 2 cut(s) 864, 1231
BspFNI CGCG 1 cut(s) 1474
BspHI TCATGA 3 cut(s) 289, 1372, 2905
BspLI GGNNCC 3 cut(s) 1788, 2122, 2148
BspMI ACCTGC 1 cut(s) 111
BspOI GCTAGC 2 cut(s) 1472, 2105
BspPI GGATC 4 cut(s) 1319, 1671, 1897, 2173
BspQI GCTCTTC 2 cut(s) 507, 1614
BsrDI GCAATG 2 cut(s) 354, 1287
BsrGI TGTACA 1 cut(s) 1300
BsrI ACTGG 9 cut(s) 12, 641, 865, 1580, 1581, 1676, 1942, 2258, 2363
BssECI CCNNGG 5 cut(s) 815, 1845, 2183, 2708, 2722
BssHII GCGCGC 1 cut(s) 1472
BssMI GATC 8 cut(s) 167, 235, 598, 1153, 1324, 1663, 1889, 2178
BssNAI GTATAC 2 cut(s) 734, 2678
BssT1I CCWWGG 3 cut(s) 1845, 2183, 2722
Bst1107I GTATAC 2 cut(s) 734, 2678
Bst2UI CCWGG 4 cut(s) 713, 738, 2247, 2781
Bst6I CTCTTC 5 cut(s) 507, 1427, 1614, 1919, 2471
BstAPI GCANNNNNTGC 2 cut(s) 2408, 2876
BstAUI TGTACA 1 cut(s) 1300
BstBAI YACGTR 1 cut(s) 2774
BstC8I GCNNGC 9 cut(s) 22, 226, 1146, 1470, 1474, 1744, 1803, 2103, 2193
BstDSI CCRYGG 2 cut(s) 1845, 2708
BstENI CCTNNNNNAGG 1 cut(s) 1251
BstF5I GGATG 8 cut(s) 358, 994, 1858, 1920, 2026, 2043, 2122, 2308
BstFNI CGCG 1 cut(s) 1474
BstHHI GCGC 2 cut(s) 1474, 1476
BstKTI GATC 8 cut(s) 170, 238, 601, 1156, 1327, 1666, 1892, 2181
BstMAI GTCTC 5 cut(s) 761, 1101, 1934, 2258, 2944
BstMBI GATC 8 cut(s) 167, 235, 598, 1153, 1324, 1663, 1889, 2178
BstNI CCWGG 4 cut(s) 713, 738, 2247, 2781
BstNSI RCATGY 3 cut(s) 28, 1746, 2101
BstSCI CCNGG 4 cut(s) 711, 736, 2245, 2779
BstSFI CTRYAG 1 cut(s) 2446
BstUI CGCG 1 cut(s) 1474
BstV2I GAAGAC 4 cut(s) 278, 1019, 1361, 1578
BstX2I RGATCY 1 cut(s) 1663
BstXI CCANNNNNNTGG 1 cut(s) 613
BstYI RGATCY 1 cut(s) 1663
BstZ17I GTATAC 2 cut(s) 734, 2678
BsuI GTATCC 2 cut(s) 567, 2370
BsuRI GGCC 2 cut(s) 864, 1231
BtgI CCRYGG 2 cut(s) 1845, 2708
BtsCI GGATG 8 cut(s) 358, 994, 1858, 1920, 2026, 2043, 2122, 2308
BtsIMutI CAGTG 3 cut(s) 5, 2265, 2498
BveI ACCTGC 1 cut(s) 111
Cac8I GCNNGC 9 cut(s) 22, 226, 1146, 1470, 1474, 1744, 1803, 2103, 2193
CaiI CAGNNNCTG 2 cut(s) 647, 2408
CciI TCATGA 3 cut(s) 289, 1372, 2905
CfoI GCGC 2 cut(s) 1474, 1476
Cfr13I GGNCC 5 cut(s) 819, 1578, 2719, 2761, 2777
CseI GACGC 2 cut(s) 743, 2197
CsiI ACCWGGT 1 cut(s) 2779
Csp6I GTAC 6 cut(s) 129, 868, 918, 1050, 1301, 1503
CviQI GTAC 6 cut(s) 129, 868, 918, 1050, 1301, 1503
DpnI GATC 8 cut(s) 169, 237, 600, 1155, 1326, 1665, 1891, 2180
DpnII GATC 8 cut(s) 167, 235, 598, 1153, 1324, 1663, 1889, 2178
DraI TTTAAA 1 cut(s) 340
EaeI YGGCCR 1 cut(s) 862
Eam1104I CTCTTC 5 cut(s) 507, 1427, 1614, 1919, 2471
EarI CTCTTC 5 cut(s) 507, 1427, 1614, 1919, 2471
Eco130I CCWWGG 3 cut(s) 1845, 2183, 2722
Eco24I GRGCYC 1 cut(s) 1763
Eco32I GATATC 4 cut(s) 83, 1285, 1769, 1947
Eco47I GGWCC 5 cut(s) 819, 1578, 2719, 2761, 2777
Eco57I CTGAAG 4 cut(s) 504, 1052, 1448, 2735
EcoNI CCTNNNNNAGG 1 cut(s) 1251
EcoO109I RGGNCCY 1 cut(s) 2719
EcoRII CCWGG 4 cut(s) 711, 736, 2245, 2779
EcoRV GATATC 4 cut(s) 83, 1285, 1769, 1947
EcoT14I CCWWGG 3 cut(s) 1845, 2183, 2722
EcoT22I ATGCAT 4 cut(s) 94, 1748, 2308, 2581
EcoT38I GRGCYC 1 cut(s) 1763
ErhI CCWWGG 3 cut(s) 1845, 2183, 2722
Esp3I CGTCTC 1 cut(s) 761
FalI AAGNNNNNCTT 6 cut(s) 1546, 1578, 2859, 2891, 2925, 2957
FaqI GGGAC 3 cut(s) 448, 2555, 2823
FauI CCCGC 1 cut(s) 2931
FauNDI CATATG 3 cut(s) 1615, 2142, 2308
FbaI TGATCA 1 cut(s) 598
FblI GTMKAC 2 cut(s) 733, 2677
FokI GGATG 8 cut(s) 365, 1001, 1865, 1927, 2030, 2033, 2129, 2315
FriOI GRGCYC 1 cut(s) 1763
FspBI CTAG 6 cut(s) 767, 1469, 1739, 1968, 2102, 2354
GlaI GCGC 2 cut(s) 1473, 1475
GsuI CTGGAG 3 cut(s) 1916, 1959, 2538
HaeIII GGCC 2 cut(s) 864, 1231
HapII CCGG 1 cut(s) 416
HgaI GACGC 2 cut(s) 743, 2197
HhaI GCGC 2 cut(s) 1474, 1476
Hin6I GCGC 2 cut(s) 1472, 1474
HinP1I GCGC 2 cut(s) 1472, 1474
HincII GTYRAC 1 cut(s) 591
HindII GTYRAC 1 cut(s) 591
HpaI GTTAAC 1 cut(s) 591
HpaII CCGG 1 cut(s) 416
HphI GGTGA 7 cut(s) 416, 536, 1144, 1385, 1715, 2144, 2909
Hpy166II GTNNAC 7 cut(s) 591, 606, 734, 1052, 1505, 2678, 2777
Hpy8I GTNNAC 7 cut(s) 591, 606, 734, 1052, 1505, 2678, 2777
HpyCH4IV ACGT 3 cut(s) 1054, 1272, 2773
HpySE526I ACGT 3 cut(s) 1054, 1272, 2773
HspAI GCGC 2 cut(s) 1472, 1474
Ksp22I TGATCA 1 cut(s) 598
KspAI GTTAAC 1 cut(s) 591
Kzo9I GATC 8 cut(s) 167, 235, 598, 1153, 1324, 1663, 1889, 2178
LguI GCTCTTC 2 cut(s) 507, 1614
LmnI GCTCC 3 cut(s) 2120, 2152, 2914
MabI ACCWGGT 1 cut(s) 2779
MaeI CTAG 6 cut(s) 767, 1469, 1739, 1968, 2102, 2354
MaeII ACGT 3 cut(s) 1054, 1272, 2773
MaeIII GTNAC 4 cut(s) 1908, 2625, 2783, 2972
MalI GATC 8 cut(s) 169, 237, 600, 1155, 1326, 1665, 1891, 2180
MboI GATC 8 cut(s) 167, 235, 598, 1153, 1324, 1663, 1889, 2178
MfeI CAATTG 1 cut(s) 930
MflI RGATCY 1 cut(s) 1663
MhlI GDGCHC 2 cut(s) 1763, 1824
MlsI TGGCCA 1 cut(s) 864
MluNI TGGCCA 1 cut(s) 864
MlyI GAGTC 1 cut(s) 1936
MmeI TCCRAC 1 cut(s) 2629
Mox20I TGGCCA 1 cut(s) 864
Mph1103I ATGCAT 4 cut(s) 94, 1748, 2308, 2581
MroXI GAANNNNTTC 1 cut(s) 489
MscI TGGCCA 1 cut(s) 864
MslI CAYNNNNRTG 1 cut(s) 987
Msp20I TGGCCA 1 cut(s) 864
MspA1I CMGCKG 5 cut(s) 20, 1636, 2386, 2451, 2926
MspI CCGG 1 cut(s) 416
MspR9I CCNGG 4 cut(s) 713, 738, 2247, 2781
MunI CAATTG 1 cut(s) 930
Mva1269I GAATGC 3 cut(s) 94, 844, 2605
MvaI CCWGG 4 cut(s) 713, 738, 2247, 2781
MvnI CGCG 1 cut(s) 1474
NcoI CCATGG 1 cut(s) 1845
NdeI CATATG 3 cut(s) 1615, 2142, 2308
NdeII GATC 8 cut(s) 167, 235, 598, 1153, 1324, 1663, 1889, 2178
NheI GCTAGC 2 cut(s) 1468, 2101
NlaIV GGNNCC 3 cut(s) 1788, 2122, 2148
NmuCI GTSAC 2 cut(s) 1908, 2972
NsiI ATGCAT 4 cut(s) 94, 1748, 2308, 2581
NspI RCATGY 3 cut(s) 28, 1746, 2101
PaeI GCATGC 1 cut(s) 1746
PagI TCATGA 3 cut(s) 289, 1372, 2905
PauI GCGCGC 1 cut(s) 1472
PciSI GCTCTTC 2 cut(s) 507, 1614
PcsI WCGNNNNNNNCGW 2 cut(s) 1051, 2650
PctI GAATGC 3 cut(s) 94, 844, 2605
PdmI GAANNNNTTC 1 cut(s) 489
PfoI TCCNGGA 1 cut(s) 711
PleI GAGTC 1 cut(s) 1935
PpsI GAGTC 1 cut(s) 1935
Ppu21I YACGTR 1 cut(s) 2774
PpuMI RGGWCCY 1 cut(s) 2719
Psp5II RGGWCCY 1 cut(s) 2719
Psp6I CCWGG 4 cut(s) 711, 736, 2245, 2779
PspGI CCWGG 4 cut(s) 711, 736, 2245, 2779
PspN4I GGNNCC 3 cut(s) 1788, 2122, 2148
PspPI GGNCC 5 cut(s) 819, 1578, 2719, 2761, 2777
PspPPI RGGWCCY 1 cut(s) 2719
PstNI CAGNNNCTG 2 cut(s) 647, 2408
PsuI RGATCY 1 cut(s) 1663
PteI GCGCGC 1 cut(s) 1472
PvuII CAGCTG 4 cut(s) 20, 1636, 2386, 2451
RsaI GTAC 6 cut(s) 130, 869, 919, 1051, 1302, 1504
RsaNI GTAC 6 cut(s) 129, 868, 918, 1050, 1301, 1503
RseI CAYNNNNRTG 1 cut(s) 987
SapI GCTCTTC 2 cut(s) 507, 1614
Sau3AI GATC 8 cut(s) 167, 235, 598, 1153, 1324, 1663, 1889, 2178
Sau96I GGNCC 5 cut(s) 819, 1578, 2719, 2761, 2777
ScaI AGTACT 2 cut(s) 130, 869
SchI GAGTC 1 cut(s) 1936
ScrFI CCNGG 4 cut(s) 713, 738, 2247, 2781
SduI GDGCHC 2 cut(s) 1763, 1824
SexAI ACCWGGT 1 cut(s) 2779
SfcI CTRYAG 1 cut(s) 2446
SinI GGWCC 5 cut(s) 819, 1578, 2719, 2761, 2777
SmiMI CAYNNNNRTG 1 cut(s) 987
SpeI ACTAGT 1 cut(s) 1967
SphI GCATGC 1 cut(s) 1746
SsiI CCGC 4 cut(s) 793, 1716, 2411, 2924
SspI AATATT 3 cut(s) 1180, 1321, 1443
SspMI CTAG 6 cut(s) 767, 1469, 1739, 1968, 2102, 2354
StyD4I CCNGG 4 cut(s) 711, 736, 2245, 2779
StyI CCWWGG 3 cut(s) 1845, 2183, 2722
TaiI ACGT 3 cut(s) 1057, 1275, 2776
TaqI TCGA 3 cut(s) 166, 1690, 2322
TaqII GACCGA 1 cut(s) 228
TatI WGTACW 3 cut(s) 128, 867, 1300
TscAI CASTG 3 cut(s) 12, 2265, 2505
TseFI GTSAC 2 cut(s) 1908, 2972
Tsp45I GTSAC 2 cut(s) 1908, 2972
TspGWI ACGGA 4 cut(s) 1013, 1927, 2020, 2387
TspRI CASTG 3 cut(s) 12, 2265, 2505
VpaK11BI GGWCC 5 cut(s) 819, 1578, 2719, 2761, 2777
XagI CCTNNNNNAGG 1 cut(s) 1251
XapI RAATTY 4 cut(s) 1037, 1352, 1831, 2889
XceI RCATGY 3 cut(s) 28, 1746, 2101
XmiI GTMKAC 2 cut(s) 733, 2677
XmnI GAANNNNTTC 1 cut(s) 489
XspI CTAG 6 cut(s) 767, 1469, 1739, 1968, 2102, 2354
ZrmI AGTACT 2 cut(s) 130, 869
Zsp2I ATGCAT 4 cut(s) 94, 1748, 2308, 2581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.