FvH4_4g33220

Clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0017660
Physical Location & Seq
Forward (+)
31517066 .. 31518442
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33220.t1

Sequence Viewer

Length: 1377 bp
ATGCAGAGGCGATTCCGGCAAGTCTTCACCGCTCTAAGAGAGCACACCTCCGTGAGCTACGCAAAGATCGCCACGGTTGGAGGGTTCTGCAACGTGGAACTCATCATCATAAAAGCAACCGCTCCGGATGACTTGCCTTTGCCGGAAAAGTACATTCAAGAGCTCTTGAAAATCTTCTCCATCTCTCCAACCACTTTGAGAGAATTCTCACTCAGCTTCTCTCGCCGCTTCAGCAAGACTCGATGCTGGAGAGTTGCACTCAAGTGCTTGATCCTCCTCCACCGTCTCCTCCGTGCAGTCCCCGACGACAGTTTGTTTCGGAGTGAGCTCCTTTGGATACGTTCCATTGGCTTGATGTCTCTCAACAACTGTCGCTTTCGCGATGATTCCTCCTCTGCTTCAGCAGACTACACAGCGTTTATCAGATCCTATGCTCAGCTCCTCGACGAAGCTCTTCATTGCTTTTTCTTGGACAACACGGCACCCCAAGAATTACCACAACTACAATATCAAGAGGAAGGAGAAGATGAACAAGAGGAAGAAGAAGATGATGAACCAAAGTTTCAGAGCTTATCAGCAAAAATGGAAGAAATGAGTCGAATGCTTGAAATGTTGCCGCAGCTACAAAGCCTGATTGATCGAGTCATGGACTGCCGGCCCACAGGGCCGGCAGCTAGAAGCTATTTGGTCCAAGTAGCCATGAAACTTCTAATTCGAGACAGCTTTGTGTGTTACACAATCTTTAGGAGGGAAATTGTTATGGTTCTTGATAGCCTATTTCAGATGCCTTATCGGAATTGCATAACGGCTTTTGGGATTTACAAGAAGGCAGCAGTGCAAGCAAATGAGCTCGGTGAATTCTACGACTGGTGTAAGGCGATGGGATTGTGTGGTGCATACGAGTATCCATTTATTGATAGAATCCCACATATTCAGATCCATGCTCTTGAGAACTTCCTCCATGGGATGTGGCAGTTGACAGATCAGTCCTCTTCCACTCCGACCTCATCGCCATCCTCTTCTTTAGTGGAGAGCTCTAGTGAAACCCCAACGGAGGACGACTGCAGCATTCTTGTTAGTACAAAATGGGAGAAGCCATTGATTCAGTTCGGTAAGGATAACGTGGACGTGAAGCCCTTAATTCGTTTTCGGAAAGAGGAAGAAGAGAAGATGTTGATTCAGTTTGAAGAGGTGGAGGAGAGCTGGGAGAGCATTCTTGAAGCTTCCATCAATGTCTCGCCTTCTCATCATCAACAAAACAACATGTTGTGCTTGTACGATCCGAACGTCGTTAACCCTTTCTACCAGTCGAGCATGATGAGGCAAAATTACCATGGTTTTGTTACCACCAACAACGCCATTCCTTCCTTAATCTAG

Protein Analysis

459

Amino Acids

52.93

Weight (kDa)

5.1

Isoelectric Point (pI)

61.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 35 - 321 5.5e-50 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017660)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g33220
malus_domestica MD13G1017400.v1.1 MD16G1015500.v1.1
prunus_persica Prupe.1G336100_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0442371
rosa_laevigata RLG00000006015
rosa_multiflora Rmu_co8473163.1_g000001 Rmu_sc0000124.1_g000044
rosa_rugosa Rorug04G0340600
rosa_samantha Rh4BG405900 Rh4CG421000 Rh4DG399000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 985
AccB1I GGYRCC 1 cut(s) 481
AccBSI CCGCTC 2 cut(s) 32, 122
AccII CGCG 1 cut(s) 381
AccIII TCCGGA 1 cut(s) 124
AciI CCGC 4 cut(s) 30, 120, 226, 617
AclWI GGATC 4 cut(s) 265, 420, 931, 1274
AcsI RAATTY 2 cut(s) 203, 857
AcuI CTGAAG 2 cut(s) 214, 384
AfaI GTAC 3 cut(s) 152, 1081, 1277
AfiI CCNNNNNNNGG 1 cut(s) 1054
AflIII ACRYGT 1 cut(s) 1263
AgsI TTSAA 5 cut(s) 158, 169, 608, 1187, 1220
AjiI CACGTC 1 cut(s) 1129
AleI CACNNNNGTG 2 cut(s) 50, 262
Alw21I GWGCWC 5 cut(s) 45, 165, 330, 852, 1037
Alw26I GTCTC 4 cut(s) 290, 363, 711, 1240
AlwI GGATC 4 cut(s) 265, 420, 931, 1274
Aor13HI TCCGGA 1 cut(s) 124
AoxI GGCC 2 cut(s) 656, 665
ApeKI GCWGC 4 cut(s) 619, 671, 830, 1065
ApoI RAATTY 2 cut(s) 203, 857
Asp700I GAANNNNTTC 2 cut(s) 173, 453
AspS9I GGNCC 3 cut(s) 657, 665, 688
AsuHPI GGTGA 2 cut(s) 19, 866
AvaII GGWCC 1 cut(s) 688
BanI GGYRCC 1 cut(s) 481
BanII GRGCYC 4 cut(s) 165, 330, 852, 1037
BbsI GAAGAC 1 cut(s) 16
Bbv12I GWGCWC 5 cut(s) 45, 165, 330, 852, 1037
BbvI GCAGC 4 cut(s) 631, 683, 842, 1077
BccI CCATC 4 cut(s) 188, 874, 1021, 1235
BceAI ACGGC 2 cut(s) 495, 822
BciVI GTATCC 2 cut(s) 330, 915
BcoDI GTCTC 4 cut(s) 290, 363, 711, 1240
BfaI CTAG 3 cut(s) 675, 1038, 1375
BfmI CTRYAG 1 cut(s) 1063
BfuI GTATCC 2 cut(s) 330, 915
BglI GCCNNNNNGGC 1 cut(s) 664
BisI GCNGC 6 cut(s) 226, 617, 620, 672, 831, 1066
BlpI GCTNAGC 1 cut(s) 435
BlsI GCNGC 6 cut(s) 227, 618, 621, 673, 832, 1067
Bme18I GGWCC 1 cut(s) 688
BmgBI CACGTC 1 cut(s) 1129
BmgT120I GGNCC 3 cut(s) 657, 665, 688
BmiI GGNNCC 1 cut(s) 483
BmsI GCATC 2 cut(s) 233, 774
BpiI GAAGAC 1 cut(s) 16
BplI GAGNNNNNCTC 4 cut(s) 32, 64, 243, 275
BpmI CTGGAG 1 cut(s) 268
Bpu1102I GCTNAGC 1 cut(s) 435
BpuEI CTTGAG 2 cut(s) 245, 968
BsaJI CCNNGG 3 cut(s) 72, 961, 1333
BsaWI WCCGGW 1 cut(s) 124
Bsc4I CCNNNNNNNGG 1 cut(s) 1054
Bse118I RCCGGY 2 cut(s) 654, 667
Bse1I ACTGG 2 cut(s) 872, 1306
Bse3DI GCAATG 1 cut(s) 457
BseAI TCCGGA 1 cut(s) 124
BseDI CCNNGG 3 cut(s) 72, 961, 1333
BseGI GGATG 3 cut(s) 133, 972, 1013
BseLI CCNNNNNNNGG 1 cut(s) 1054
BseMI GCAATG 1 cut(s) 457
BseMII CTCAG 2 cut(s) 226, 449
BseNI ACTGG 2 cut(s) 872, 1306
BseRI GAGGAG 5 cut(s) 266, 278, 382, 431, 1211
BseXI GCAGC 4 cut(s) 631, 683, 842, 1077
BseYI CCCAGC 1 cut(s) 1203
BsgI GTGCAG 1 cut(s) 315
Bsh1236I CGCG 1 cut(s) 381
BshFI GGCC 2 cut(s) 658, 667
BshNI GGYRCC 1 cut(s) 481
BsiHKAI GWGCWC 5 cut(s) 45, 165, 330, 852, 1037
BsiSI CCGG 5 cut(s) 16, 125, 143, 655, 668
BslFI GGGAC 1 cut(s) 284
BslI CCNNNNNNNGG 1 cut(s) 1054
BsmAI GTCTC 4 cut(s) 290, 363, 711, 1240
BsmBI CGTCTC 1 cut(s) 290
BsmFI GGGAC 1 cut(s) 284
BsmI GAATGC 3 cut(s) 606, 1068, 1212
BsnI GGCC 2 cut(s) 658, 667
Bsp1286I GDGCHC 5 cut(s) 45, 165, 330, 852, 1037
Bsp13I TCCGGA 1 cut(s) 124
Bsp143I GATC 7 cut(s) 66, 270, 425, 637, 936, 982, 1279
Bsp1720I GCTNAGC 1 cut(s) 435
Bsp19I CCATGG 2 cut(s) 961, 1333
Bsp68I TCGCGA 1 cut(s) 381
BspACI CCGC 4 cut(s) 30, 120, 226, 617
BspANI GGCC 2 cut(s) 658, 667
BspCNI CTCAG 2 cut(s) 225, 448
BspEI TCCGGA 1 cut(s) 124
BspFNI CGCG 1 cut(s) 381
BspLI GGNNCC 1 cut(s) 483
BspMAI CTGCAG 1 cut(s) 1067
BspPI GGATC 4 cut(s) 265, 420, 931, 1274
BspQI GCTCTTC 1 cut(s) 459
BspT107I GGYRCC 1 cut(s) 481
BsrBI CCGCTC 2 cut(s) 32, 122
BsrDI GCAATG 1 cut(s) 457
BsrFI RCCGGY 2 cut(s) 654, 667
BsrI ACTGG 2 cut(s) 872, 1306
BssAI RCCGGY 2 cut(s) 654, 667
BssECI CCNNGG 3 cut(s) 72, 961, 1333
BssMI GATC 7 cut(s) 66, 270, 425, 637, 936, 982, 1279
BssT1I CCWWGG 2 cut(s) 961, 1333
Bst4CI ACNGT 4 cut(s) 76, 284, 311, 371
Bst6I CTCTTC 5 cut(s) 459, 997, 1024, 1158, 1182
BstC8I GCNNGC 3 cut(s) 656, 669, 840
BstDEI CTNAG 3 cut(s) 35, 212, 435
BstDSI CCRYGG 3 cut(s) 72, 961, 1333
BstF5I GGATG 3 cut(s) 133, 972, 1013
BstFNI CGCG 1 cut(s) 381
BstKTI GATC 7 cut(s) 69, 273, 428, 640, 939, 985, 1282
BstMAI GTCTC 4 cut(s) 290, 363, 711, 1240
BstMBI GATC 7 cut(s) 66, 270, 425, 637, 936, 982, 1279
BstMWI GCNNNNNNNGC 7 cut(s) 16, 68, 222, 231, 664, 839, 1209
BstNSI RCATGY 1 cut(s) 1267
BstSFI CTRYAG 1 cut(s) 1063
BstUI CGCG 1 cut(s) 381
BstV1I GCAGC 4 cut(s) 631, 683, 842, 1077
BstV2I GAAGAC 1 cut(s) 16
BstX2I RGATCY 2 cut(s) 425, 936
BstYI RGATCY 2 cut(s) 425, 936
BsuI GTATCC 2 cut(s) 330, 915
BsuRI GGCC 2 cut(s) 658, 667
BtgI CCRYGG 3 cut(s) 72, 961, 1333
BtgZI GCGATG 3 cut(s) 396, 893, 993
BtrI CACGTC 1 cut(s) 1129
BtsCI GGATG 3 cut(s) 133, 972, 1013
BtsI GCAGTG 1 cut(s) 840
BtsIMutI CAGTG 1 cut(s) 840
BtuMI TCGCGA 1 cut(s) 381
Cac8I GCNNGC 3 cut(s) 656, 669, 840
Cfr10I RCCGGY 2 cut(s) 654, 667
Cfr13I GGNCC 3 cut(s) 657, 665, 688
Csp6I GTAC 3 cut(s) 151, 1080, 1276
CviAII CATG 7 cut(s) 646, 700, 941, 962, 1264, 1315, 1334
CviQI GTAC 3 cut(s) 151, 1080, 1276
DdeI CTNAG 3 cut(s) 35, 212, 435
DpnI GATC 7 cut(s) 68, 272, 427, 639, 938, 984, 1281
DpnII GATC 7 cut(s) 66, 270, 425, 637, 936, 982, 1279
DrdI GACNNNNNNGTC 1 cut(s) 985
DseDI GACNNNNNNGTC 1 cut(s) 985
Eam1104I CTCTTC 5 cut(s) 459, 997, 1024, 1158, 1182
EarI CTCTTC 5 cut(s) 459, 997, 1024, 1158, 1182
Ecl136II GAGCTC 4 cut(s) 163, 328, 850, 1035
Eco130I CCWWGG 2 cut(s) 961, 1333
Eco24I GRGCYC 4 cut(s) 165, 330, 852, 1037
Eco47I GGWCC 1 cut(s) 688
Eco53kI GAGCTC 4 cut(s) 163, 328, 850, 1035
Eco57I CTGAAG 2 cut(s) 214, 384
EcoICRI GAGCTC 4 cut(s) 163, 328, 850, 1035
EcoRI GAATTC 2 cut(s) 203, 857
EcoT14I CCWWGG 2 cut(s) 961, 1333
EcoT38I GRGCYC 4 cut(s) 165, 330, 852, 1037
ErhI CCWWGG 2 cut(s) 961, 1333
Esp3I CGTCTC 1 cut(s) 290
FaeI CATG 7 cut(s) 649, 703, 944, 965, 1267, 1318, 1337
FaqI GGGAC 1 cut(s) 284
FatI CATG 7 cut(s) 645, 699, 940, 961, 1263, 1314, 1333
Fnu4HI GCNGC 6 cut(s) 226, 617, 620, 672, 831, 1066
FokI GGATG 3 cut(s) 140, 979, 1000
FriOI GRGCYC 4 cut(s) 165, 330, 852, 1037
Fsp4HI GCNGC 6 cut(s) 226, 617, 620, 672, 831, 1066
FspBI CTAG 3 cut(s) 675, 1038, 1375
GluI GCNGC 6 cut(s) 226, 617, 620, 672, 831, 1066
GsaI CCCAGC 1 cut(s) 1207
GsuI CTGGAG 1 cut(s) 268
HaeIII GGCC 2 cut(s) 658, 667
HapII CCGG 5 cut(s) 16, 125, 143, 655, 668
Hin1II CATG 7 cut(s) 649, 703, 944, 965, 1267, 1318, 1337
HincII GTYRAC 2 cut(s) 978, 1294
HindII GTYRAC 2 cut(s) 978, 1294
HindIII AAGCTT 1 cut(s) 1221
HinfI GANTC 8 cut(s) 12, 238, 386, 595, 642, 921, 1102, 1177
HpaI GTTAAC 1 cut(s) 1294
HpaII CCGG 5 cut(s) 16, 125, 143, 655, 668
HphI GGTGA 2 cut(s) 19, 866
Hpy166II GTNNAC 3 cut(s) 978, 1126, 1294
Hpy188I TCNGA 9 cut(s) 321, 425, 567, 783, 795, 936, 1002, 1152, 1284
Hpy188III TCNNGA 9 cut(s) 125, 158, 166, 380, 512, 716, 767, 947, 1217
Hpy8I GTNNAC 3 cut(s) 978, 1126, 1294
Hpy99I CGWCG 3 cut(s) 308, 449, 1292
HpyAV CCTTC 4 cut(s) 512, 820, 1251, 1374
HpyCH4III ACNGT 4 cut(s) 76, 284, 311, 371
HpyCH4IV ACGT 5 cut(s) 93, 340, 1122, 1128, 1287
HpyCH4V TGCA 8 cut(s) 4, 90, 257, 296, 801, 838, 896, 1065
HpyF10VI GCNNNNNNNGC 7 cut(s) 16, 68, 222, 231, 664, 839, 1209
HpyF3I CTNAG 3 cut(s) 35, 212, 435
HpySE526I ACGT 5 cut(s) 93, 340, 1122, 1128, 1287
Hsp92II CATG 7 cut(s) 649, 703, 944, 965, 1267, 1318, 1337
Kpn2I TCCGGA 1 cut(s) 124
KroI GCCGGC 2 cut(s) 654, 667
KroNI GCCGGC 2 cut(s) 656, 669
KspAI GTTAAC 1 cut(s) 1294
Kzo9I GATC 7 cut(s) 66, 270, 425, 637, 936, 982, 1279
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 3 cut(s) 127, 333, 444
Lsp1109I GCAGC 4 cut(s) 631, 683, 842, 1077
LweI GCATC 2 cut(s) 233, 774
MaeI CTAG 3 cut(s) 675, 1038, 1375
MaeII ACGT 5 cut(s) 93, 340, 1122, 1128, 1287
MaeIII GTNAC 2 cut(s) 731, 1342
MalI GATC 7 cut(s) 68, 272, 427, 639, 938, 984, 1281
MbiI CCGCTC 2 cut(s) 32, 122
MboI GATC 7 cut(s) 66, 270, 425, 637, 936, 982, 1279
MflI RGATCY 2 cut(s) 425, 936
MhlI GDGCHC 5 cut(s) 45, 165, 330, 852, 1037
MluCI AATT 8 cut(s) 203, 491, 711, 753, 796, 857, 1140, 1327
MlyI GAGTC 3 cut(s) 232, 604, 651
MmeI TCCRAC 3 cut(s) 58, 212, 1025
MroI TCCGGA 1 cut(s) 124
MroNI GCCGGC 2 cut(s) 654, 667
MroXI GAANNNNTTC 2 cut(s) 173, 453
MseI TTAA 3 cut(s) 1139, 1293, 1370
MslI CAYNNNNRTG 2 cut(s) 50, 262
MspI CCGG 5 cut(s) 16, 125, 143, 655, 668
Mva1269I GAATGC 3 cut(s) 606, 1068, 1212
MvnI CGCG 1 cut(s) 381
MwoI GCNNNNNNNGC 7 cut(s) 16, 68, 222, 231, 664, 839, 1209
NaeI GCCGGC 2 cut(s) 656, 669
NcoI CCATGG 2 cut(s) 961, 1333
NdeII GATC 7 cut(s) 66, 270, 425, 637, 936, 982, 1279
NgoMIV GCCGGC 2 cut(s) 654, 667
NlaIII CATG 7 cut(s) 649, 703, 944, 965, 1267, 1318, 1337
NlaIV GGNNCC 1 cut(s) 483
NruI TCGCGA 1 cut(s) 381
NspI RCATGY 1 cut(s) 1267
OliI CACNNNNGTG 2 cut(s) 50, 262
PciI ACATGT 1 cut(s) 1263
PciSI GCTCTTC 1 cut(s) 459
PcsI WCGNNNNNNNCGW 1 cut(s) 1284
PctI GAATGC 3 cut(s) 606, 1068, 1212
PdiI GCCGGC 2 cut(s) 656, 669
PdmI GAANNNNTTC 2 cut(s) 173, 453
PfeI GAWTC 5 cut(s) 12, 386, 921, 1102, 1177
PkrI GCNGC 6 cut(s) 227, 618, 621, 673, 832, 1067
PleI GAGTC 3 cut(s) 232, 603, 650
PpsI GAGTC 3 cut(s) 232, 603, 650
PscI ACATGT 1 cut(s) 1263
Psp124BI GAGCTC 4 cut(s) 165, 330, 852, 1037
PspFI CCCAGC 1 cut(s) 1203
PspN4I GGNNCC 1 cut(s) 483
PspPI GGNCC 3 cut(s) 657, 665, 688
PstI CTGCAG 1 cut(s) 1067
PsuI RGATCY 2 cut(s) 425, 936
RruI TCGCGA 1 cut(s) 381
RsaI GTAC 3 cut(s) 152, 1081, 1277
RsaNI GTAC 3 cut(s) 151, 1080, 1276
RseI CAYNNNNRTG 2 cut(s) 50, 262
SacI GAGCTC 4 cut(s) 165, 330, 852, 1037
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 3 cut(s) 1139, 1293, 1370
SatI GCNGC 6 cut(s) 226, 617, 620, 672, 831, 1066
Sau3AI GATC 7 cut(s) 66, 270, 425, 637, 936, 982, 1279
Sau96I GGNCC 3 cut(s) 657, 665, 688
SchI GAGTC 3 cut(s) 232, 604, 651
SduI GDGCHC 5 cut(s) 45, 165, 330, 852, 1037
SfaNI GCATC 2 cut(s) 233, 774
SfcI CTRYAG 1 cut(s) 1063
SfiI GGCCNNNNNGGCC 1 cut(s) 664
SinI GGWCC 1 cut(s) 688
SmiMI CAYNNNNRTG 2 cut(s) 50, 262
SmlI CTYRAG 2 cut(s) 260, 947
SmoI CTYRAG 2 cut(s) 260, 947
Sse9I AATT 8 cut(s) 203, 491, 711, 753, 796, 857, 1140, 1327
SsiI CCGC 4 cut(s) 30, 120, 226, 617
SspMI CTAG 3 cut(s) 675, 1038, 1375
SstI GAGCTC 4 cut(s) 165, 330, 852, 1037
StyI CCWWGG 2 cut(s) 961, 1333
TaaI ACNGT 4 cut(s) 76, 284, 311, 371
TaiI ACGT 5 cut(s) 96, 343, 1125, 1131, 1290
TaqI TCGA 6 cut(s) 241, 444, 598, 640, 715, 1310
TasI AATT 8 cut(s) 203, 491, 711, 753, 796, 857, 1140, 1327
TatI WGTACW 2 cut(s) 150, 1079
TauI GCSGC 2 cut(s) 228, 619
TfiI GAWTC 5 cut(s) 12, 386, 921, 1102, 1177
Tru1I TTAA 3 cut(s) 1139, 1293, 1370
Tru9I TTAA 3 cut(s) 1139, 1293, 1370
TscAI CASTG 1 cut(s) 840
TseI GCWGC 4 cut(s) 619, 671, 830, 1065
TspDTI ATGAA 4 cut(s) 446, 543, 567, 716
TspGWI ACGGA 3 cut(s) 40, 281, 1067
TspRI CASTG 1 cut(s) 840
VpaK11BI GGWCC 1 cut(s) 688
XapI RAATTY 2 cut(s) 203, 857
XceI RCATGY 1 cut(s) 1267
XmnI GAANNNNTTC 2 cut(s) 173, 453
XspI CTAG 3 cut(s) 675, 1038, 1375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.