RchiOBHm_Chr4g0442371

Clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0017660
Physical Location & Seq
Forward (+)
63986482 .. 63987837
1356 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41013

Sequence Viewer

Length: 1356 bp
ATGCAGAGGCGATTCCGGCAAGTCTTCACCGCTCTAAAAGAGCACAGCTCCGTGAGCTACGCCAAGATCGCCACGGTTGGAGGGTTCTGCAACGTGGAGTTCATCATCATAAAAGCAACAGCTCCGGATGACTTACCGTTGCCGGAAAAGTACATTCAAGAGCTCTTGAAAATCTTCTCCATCTCTCCATCCACCCTGAGTGACTTCTCACTCAGCTTCTCTCGCCGCTTCGGCAAGACTCGATGCTGGAGAGTTGCACTCAAGTGCTTGATCCTCCTCCACCGTCTGCTCCGTGCAGTGCCCGACGACAGTGAGTTTAGGAGTGAGCTCCTTTGGACACGTTCCATTGGCTTGCTGTCCCTCAACGACTGTCGCTTCCGTGACGATTCCTCCTCGGCTTCAGCAGACTACACAGCCTTTGTGAGATCCTATGCTCAGCTCCTTGACGAAGCTCTTCATTGCTTTTGCTTGGATAACGAGGCACCCGAAGAACCACAAAAACAATATCAAGAGGAAGGAGAAGAAGGAGAAGGAGAAGATGAACAAGAGGAAGAAGATGATGAACCGCAATTTCAGAGCTTATCAACCAAAATGGAAGAAATTAGTCGAATGCTTGAAATGTTGCCGCAGCTACAAAGCCTAACTGATCGAGTCATGGACTGCCGGCCAACTGGGCCGGCAGCTAAAAGCTTCTTGGTCCAAATGGCCATGAAACTTCTCATTCGAGACAGCTTTGTGTTTTACACAATCTTTAGGAGGGAAATTGTTATGGTTCTTGATAGCCTCTTTCAGATGCCTTATCGGAACTGCATATCAGCTTTTGAGATTTACAAGAAGGCAGCAGTGCAAGCAAATGACCTTTGTGAATTCTACGACTGGTGTAAGGCAATGGGATTGTGTGGTGCATATGAGTATCCATTTATCGATCGAATCCCACATATTCAAATCCATGCTCTAGAGAACTTCCTCCATGGAATGTGGCAGTTGACAGATCAGTCCTCTTCCACGCCGACCTCATCGTCATCCTCTTCTTTGGTGGAGTGCTCTAGTGAAACTGTAACGGAGGACGACAGCATTCTTGTTAGTACAAAATGGGAGAAGCCATTGATTCAGTTCGGTGGGGATCACGAGAACGAAAAGGCCTTAATTCTGTTTGGGAAAGATGCGGAGGAGAAGATGTTGATTCAGTTTGAAGAAGTGGAGGAGAGCTGGGAGAGCATTCTGGAAGCTTCCATCAATGTCTCACCTTCTTATCAACAAAACAATATGTTGTGCTTGTACAATCCGAACGTCGTTAATCCTTTCTATCACCATGGATTTGTTACAACCAACAACGCCATTCCTTCCTTAATCTAG

Protein Analysis

451

Amino Acids

51.72

Weight (kDa)

4.73

Isoelectric Point (pI)

59.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 35 - 324 4.5e-48 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017660)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g33220
malus_domestica MD13G1017400.v1.1 MD16G1015500.v1.1
prunus_persica Prupe.1G336100_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0442371
rosa_laevigata RLG00000006015
rosa_multiflora Rmu_co8473163.1_g000001 Rmu_sc0000124.1_g000044
rosa_rugosa Rorug04G0340600
rosa_samantha Rh4BG405900 Rh4CG421000 Rh4DG399000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 994, 1018
AccB1I GGYRCC 1 cut(s) 481
AccBSI CCGCTC 1 cut(s) 32
AccIII TCCGGA 1 cut(s) 124
AciI CCGC 5 cut(s) 30, 226, 566, 626, 1166
AclWI GGATC 3 cut(s) 265, 420, 1131
AcoI YGGCCR 2 cut(s) 665, 705
AcsI RAATTY 1 cut(s) 866
AcuI CTGAAG 1 cut(s) 384
AfaI GTAC 3 cut(s) 152, 1087, 1280
AflIII ACRYGT 1 cut(s) 338
AgsI TTSAA 5 cut(s) 158, 169, 617, 944, 1193
AleI CACNNNNGTG 1 cut(s) 262
Alw21I GWGCWC 4 cut(s) 45, 165, 330, 1046
Alw26I GTCTC 2 cut(s) 720, 1246
AlwI GGATC 3 cut(s) 265, 420, 1131
Aor13HI TCCGGA 1 cut(s) 124
AoxI GGCC 4 cut(s) 665, 674, 705, 1140
ApeKI GCWGC 3 cut(s) 628, 680, 839
ApoI RAATTY 1 cut(s) 866
Asp700I GAANNNNTTC 2 cut(s) 173, 453
AspS9I GGNCC 2 cut(s) 674, 697
AsuHPI GGTGA 3 cut(s) 19, 1236, 1301
AvaII GGWCC 1 cut(s) 697
BaeGI GKGCMC 1 cut(s) 303
BalI TGGCCA 1 cut(s) 707
BanI GGYRCC 1 cut(s) 481
BanII GRGCYC 2 cut(s) 165, 330
BauI CACGAG 1 cut(s) 1127
BbsI GAAGAC 1 cut(s) 16
Bbv12I GWGCWC 4 cut(s) 45, 165, 330, 1046
BbvI GCAGC 3 cut(s) 640, 692, 851
BccI CCATC 3 cut(s) 188, 196, 1241
BciVI GTATCC 1 cut(s) 924
BcoDI GTCTC 2 cut(s) 720, 1246
BfaI CTAG 3 cut(s) 956, 1047, 1354
BfuI GTATCC 1 cut(s) 924
BglI GCCNNNNNGGC 2 cut(s) 231, 673
BisI GCNGC 5 cut(s) 226, 626, 629, 681, 840
BlpI GCTNAGC 1 cut(s) 435
BlsI GCNGC 5 cut(s) 227, 627, 630, 682, 841
Bme18I GGWCC 1 cut(s) 697
BmgT120I GGNCC 2 cut(s) 674, 697
BmiI GGNNCC 1 cut(s) 483
BmrI ACTGGG 1 cut(s) 681
BmsI GCATC 3 cut(s) 233, 783, 1153
BmuI ACTGGG 1 cut(s) 681
BpiI GAAGAC 1 cut(s) 16
BplI GAGNNNNNCTC 4 cut(s) 32, 64, 243, 275
BpmI CTGGAG 1 cut(s) 268
Bpu1102I GCTNAGC 1 cut(s) 435
BpuEI CTTGAG 1 cut(s) 245
Bsa29I ATCGAT 1 cut(s) 924
BsaJI CCNNGG 4 cut(s) 72, 393, 970, 1312
BsaWI WCCGGW 1 cut(s) 124
BsaXI ACNNNNNCTCC 4 cut(s) 374, 404, 1163, 1193
Bse118I RCCGGY 2 cut(s) 663, 676
Bse1I ACTGG 2 cut(s) 676, 881
Bse3DI GCAATG 2 cut(s) 457, 894
BseAI TCCGGA 1 cut(s) 124
BseCI ATCGAT 1 cut(s) 924
BseDI CCNNGG 4 cut(s) 72, 393, 970, 1312
BseGI GGATG 3 cut(s) 133, 188, 1022
BseMI GCAATG 2 cut(s) 457, 894
BseMII CTCAG 3 cut(s) 188, 226, 449
BseNI ACTGG 2 cut(s) 676, 881
BseRI GAGGAG 4 cut(s) 266, 382, 1184, 1217
BseSI GKGCMC 1 cut(s) 303
BseXI GCAGC 3 cut(s) 640, 692, 851
BseYI CCCAGC 1 cut(s) 1209
BsgI GTGCAG 1 cut(s) 315
Bsh1285I CGRYCG 1 cut(s) 928
BshFI GGCC 4 cut(s) 667, 676, 707, 1142
BshNI GGYRCC 1 cut(s) 481
BshVI ATCGAT 1 cut(s) 924
BsiEI CGRYCG 1 cut(s) 928
BsiHKAI GWGCWC 4 cut(s) 45, 165, 330, 1046
BsiSI CCGG 5 cut(s) 16, 125, 143, 664, 677
BslFI GGGAC 1 cut(s) 343
BsmAI GTCTC 2 cut(s) 720, 1246
BsmFI GGGAC 1 cut(s) 343
BsmI GAATGC 3 cut(s) 615, 1074, 1218
BsnI GGCC 4 cut(s) 667, 676, 707, 1142
Bsp1286I GDGCHC 5 cut(s) 45, 165, 303, 330, 1046
Bsp13I TCCGGA 1 cut(s) 124
Bsp1407I TGTACA 1 cut(s) 1278
Bsp143I GATC 7 cut(s) 66, 270, 425, 646, 925, 991, 1123
Bsp1720I GCTNAGC 1 cut(s) 435
Bsp19I CCATGG 2 cut(s) 970, 1312
BspACI CCGC 5 cut(s) 30, 226, 566, 626, 1166
BspANI GGCC 4 cut(s) 667, 676, 707, 1142
BspCNI CTCAG 3 cut(s) 189, 225, 448
BspDI ATCGAT 1 cut(s) 924
BspEI TCCGGA 1 cut(s) 124
BspLI GGNNCC 1 cut(s) 483
BspPI GGATC 3 cut(s) 265, 420, 1131
BspQI GCTCTTC 1 cut(s) 459
BspT107I GGYRCC 1 cut(s) 481
BsrBI CCGCTC 1 cut(s) 32
BsrDI GCAATG 2 cut(s) 457, 894
BsrFI RCCGGY 2 cut(s) 663, 676
BsrGI TGTACA 1 cut(s) 1278
BsrI ACTGG 2 cut(s) 676, 881
BssAI RCCGGY 2 cut(s) 663, 676
BssECI CCNNGG 4 cut(s) 72, 393, 970, 1312
BssMI GATC 7 cut(s) 66, 270, 425, 646, 925, 991, 1123
BssSI CACGAG 1 cut(s) 1127
BssT1I CCWWGG 2 cut(s) 970, 1312
Bst2BI CACGAG 1 cut(s) 1127
Bst4CI ACNGT 6 cut(s) 76, 138, 284, 311, 371, 1057
Bst6I CTCTTC 3 cut(s) 459, 1006, 1033
BstAUI TGTACA 1 cut(s) 1278
BstC8I GCNNGC 4 cut(s) 353, 665, 678, 849
BstDEI CTNAG 3 cut(s) 197, 212, 435
BstDSI CCRYGG 3 cut(s) 72, 970, 1312
BstF5I GGATG 3 cut(s) 133, 188, 1022
BstKTI GATC 7 cut(s) 69, 273, 428, 649, 928, 994, 1126
BstMAI GTCTC 2 cut(s) 720, 1246
BstMBI GATC 7 cut(s) 66, 270, 425, 646, 925, 991, 1123
BstMCI CGRYCG 1 cut(s) 928
BstMWI GCNNNNNNNGC 8 cut(s) 16, 54, 68, 222, 231, 673, 848, 1215
BstSLI GKGCMC 1 cut(s) 303
BstV1I GCAGC 3 cut(s) 640, 692, 851
BstV2I GAAGAC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 425
BstYI RGATCY 1 cut(s) 425
Bsu15I ATCGAT 1 cut(s) 924
BsuI GTATCC 1 cut(s) 924
BsuRI GGCC 4 cut(s) 667, 676, 707, 1142
BsuTUI ATCGAT 1 cut(s) 924
BtgI CCRYGG 3 cut(s) 72, 970, 1312
BtsCI GGATG 3 cut(s) 133, 188, 1022
BtsI GCAGTG 2 cut(s) 303, 849
BtsIMutI CAGTG 3 cut(s) 303, 316, 849
Cac8I GCNNGC 4 cut(s) 353, 665, 678, 849
Cfr10I RCCGGY 2 cut(s) 663, 676
Cfr13I GGNCC 2 cut(s) 674, 697
ClaI ATCGAT 1 cut(s) 924
Csp6I GTAC 3 cut(s) 151, 1086, 1279
CviAII CATG 5 cut(s) 655, 709, 950, 971, 1313
CviQI GTAC 3 cut(s) 151, 1086, 1279
DdeI CTNAG 3 cut(s) 197, 212, 435
DpnI GATC 7 cut(s) 68, 272, 427, 648, 927, 993, 1125
DpnII GATC 7 cut(s) 66, 270, 425, 646, 925, 991, 1123
DrdI GACNNNNNNGTC 2 cut(s) 994, 1018
DseDI GACNNNNNNGTC 2 cut(s) 994, 1018
EaeI YGGCCR 2 cut(s) 665, 705
Eam1104I CTCTTC 3 cut(s) 459, 1006, 1033
EarI CTCTTC 3 cut(s) 459, 1006, 1033
Ecl136II GAGCTC 2 cut(s) 163, 328
Eco130I CCWWGG 2 cut(s) 970, 1312
Eco147I AGGCCT 1 cut(s) 1142
Eco24I GRGCYC 2 cut(s) 165, 330
Eco47I GGWCC 1 cut(s) 697
Eco53kI GAGCTC 2 cut(s) 163, 328
Eco57I CTGAAG 1 cut(s) 384
EcoICRI GAGCTC 2 cut(s) 163, 328
EcoRI GAATTC 1 cut(s) 866
EcoT14I CCWWGG 2 cut(s) 970, 1312
EcoT38I GRGCYC 2 cut(s) 165, 330
ErhI CCWWGG 2 cut(s) 970, 1312
FaeI CATG 5 cut(s) 658, 712, 953, 974, 1316
FaqI GGGAC 1 cut(s) 343
FatI CATG 5 cut(s) 654, 708, 949, 970, 1312
FauNDI CATATG 1 cut(s) 907
Fnu4HI GCNGC 5 cut(s) 226, 626, 629, 681, 840
FokI GGATG 3 cut(s) 140, 175, 1009
FriOI GRGCYC 2 cut(s) 165, 330
Fsp4HI GCNGC 5 cut(s) 226, 626, 629, 681, 840
FspBI CTAG 3 cut(s) 956, 1047, 1354
GluI GCNGC 5 cut(s) 226, 626, 629, 681, 840
GsaI CCCAGC 1 cut(s) 1213
GsuI CTGGAG 1 cut(s) 268
HaeIII GGCC 4 cut(s) 667, 676, 707, 1142
HapII CCGG 5 cut(s) 16, 125, 143, 664, 677
Hin1II CATG 5 cut(s) 658, 712, 953, 974, 1316
HincII GTYRAC 1 cut(s) 987
HindII GTYRAC 1 cut(s) 987
HindIII AAGCTT 2 cut(s) 688, 1227
HinfI GANTC 7 cut(s) 12, 238, 386, 651, 930, 1108, 1183
HpaII CCGG 5 cut(s) 16, 125, 143, 664, 677
HphI GGTGA 3 cut(s) 19, 1236, 1301
Hpy166II GTNNAC 1 cut(s) 987
Hpy188I TCNGA 4 cut(s) 576, 792, 804, 1287
Hpy188III TCNNGA 9 cut(s) 125, 158, 166, 509, 725, 776, 956, 1127, 1223
Hpy8I GTNNAC 1 cut(s) 987
Hpy99I CGWCG 2 cut(s) 308, 1295
HpyAV CCTTC 6 cut(s) 509, 518, 524, 829, 1257, 1353
HpyCH4III ACNGT 6 cut(s) 76, 138, 284, 311, 371, 1057
HpyCH4IV ACGT 3 cut(s) 93, 340, 1290
HpyCH4V TGCA 7 cut(s) 4, 90, 257, 296, 810, 847, 905
HpyF10VI GCNNNNNNNGC 8 cut(s) 16, 54, 68, 222, 231, 673, 848, 1215
HpyF3I CTNAG 3 cut(s) 197, 212, 435
HpySE526I ACGT 3 cut(s) 93, 340, 1290
Hsp92II CATG 5 cut(s) 658, 712, 953, 974, 1316
Kpn2I TCCGGA 1 cut(s) 124
KroI GCCGGC 2 cut(s) 663, 676
KroNI GCCGGC 2 cut(s) 665, 678
Kzo9I GATC 7 cut(s) 66, 270, 425, 646, 925, 991, 1123
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 5 cut(s) 53, 127, 294, 333, 444
Lsp1109I GCAGC 3 cut(s) 640, 692, 851
LweI GCATC 3 cut(s) 233, 783, 1153
MaeI CTAG 3 cut(s) 956, 1047, 1354
MaeII ACGT 3 cut(s) 93, 340, 1290
MaeIII GTNAC 4 cut(s) 200, 380, 1057, 1321
MalI GATC 7 cut(s) 68, 272, 427, 648, 927, 993, 1125
MbiI CCGCTC 1 cut(s) 32
MboI GATC 7 cut(s) 66, 270, 425, 646, 925, 991, 1123
MflI RGATCY 1 cut(s) 425
MhlI GDGCHC 5 cut(s) 45, 165, 303, 330, 1046
MlsI TGGCCA 1 cut(s) 707
MluCI AATT 5 cut(s) 569, 600, 762, 866, 1146
MluNI TGGCCA 1 cut(s) 707
MlyI GAGTC 2 cut(s) 232, 660
MmeI TCCRAC 1 cut(s) 58
Mox20I TGGCCA 1 cut(s) 707
MroI TCCGGA 1 cut(s) 124
MroNI GCCGGC 2 cut(s) 663, 676
MroXI GAANNNNTTC 2 cut(s) 173, 453
MscI TGGCCA 1 cut(s) 707
MseI TTAA 3 cut(s) 1145, 1296, 1349
MslI CAYNNNNRTG 1 cut(s) 262
Msp20I TGGCCA 1 cut(s) 707
MspI CCGG 5 cut(s) 16, 125, 143, 664, 677
Mva1269I GAATGC 3 cut(s) 615, 1074, 1218
MwoI GCNNNNNNNGC 8 cut(s) 16, 54, 68, 222, 231, 673, 848, 1215
NaeI GCCGGC 2 cut(s) 665, 678
NcoI CCATGG 2 cut(s) 970, 1312
NdeI CATATG 1 cut(s) 907
NdeII GATC 7 cut(s) 66, 270, 425, 646, 925, 991, 1123
NgoMIV GCCGGC 2 cut(s) 663, 676
NlaIII CATG 5 cut(s) 658, 712, 953, 974, 1316
NlaIV GGNNCC 1 cut(s) 483
NmeAIII GCCGAG 1 cut(s) 374
NmuCI GTSAC 2 cut(s) 200, 380
OliI CACNNNNGTG 1 cut(s) 262
PceI AGGCCT 1 cut(s) 1142
PciSI GCTCTTC 1 cut(s) 459
PcsI WCGNNNNNNNCGW 1 cut(s) 483
PctI GAATGC 3 cut(s) 615, 1074, 1218
PdiI GCCGGC 2 cut(s) 665, 678
PdmI GAANNNNTTC 2 cut(s) 173, 453
PfeI GAWTC 5 cut(s) 12, 386, 930, 1108, 1183
PkrI GCNGC 5 cut(s) 227, 627, 630, 682, 841
Ple19I CGATCG 1 cut(s) 928
PleI GAGTC 2 cut(s) 232, 659
PpsI GAGTC 2 cut(s) 232, 659
Psp124BI GAGCTC 2 cut(s) 165, 330
PspFI CCCAGC 1 cut(s) 1209
PspN4I GGNNCC 1 cut(s) 483
PspPI GGNCC 2 cut(s) 674, 697
PsuI RGATCY 1 cut(s) 425
PvuI CGATCG 1 cut(s) 928
RsaI GTAC 3 cut(s) 152, 1087, 1280
RsaNI GTAC 3 cut(s) 151, 1086, 1279
RseI CAYNNNNRTG 1 cut(s) 262
SacI GAGCTC 2 cut(s) 165, 330
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 3 cut(s) 1145, 1296, 1349
SatI GCNGC 5 cut(s) 226, 626, 629, 681, 840
Sau3AI GATC 7 cut(s) 66, 270, 425, 646, 925, 991, 1123
Sau96I GGNCC 2 cut(s) 674, 697
SchI GAGTC 2 cut(s) 232, 660
SduI GDGCHC 5 cut(s) 45, 165, 303, 330, 1046
SfaNI GCATC 3 cut(s) 233, 783, 1153
SfiI GGCCNNNNNGGCC 1 cut(s) 673
SinI GGWCC 1 cut(s) 697
SmiMI CAYNNNNRTG 1 cut(s) 262
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 5 cut(s) 569, 600, 762, 866, 1146
SseBI AGGCCT 1 cut(s) 1142
SsiI CCGC 5 cut(s) 30, 226, 566, 626, 1166
SspMI CTAG 3 cut(s) 956, 1047, 1354
SstI GAGCTC 2 cut(s) 165, 330
StuI AGGCCT 1 cut(s) 1142
StyI CCWWGG 2 cut(s) 970, 1312
TaaI ACNGT 6 cut(s) 76, 138, 284, 311, 371, 1057
TaiI ACGT 3 cut(s) 96, 343, 1293
TaqI TCGA 6 cut(s) 241, 607, 649, 724, 924, 928
TasI AATT 5 cut(s) 569, 600, 762, 866, 1146
TatI WGTACW 3 cut(s) 150, 1085, 1278
TauI GCSGC 2 cut(s) 228, 628
TfiI GAWTC 5 cut(s) 12, 386, 930, 1108, 1183
Tru1I TTAA 3 cut(s) 1145, 1296, 1349
Tru9I TTAA 3 cut(s) 1145, 1296, 1349
TscAI CASTG 3 cut(s) 303, 316, 849
TseFI GTSAC 2 cut(s) 200, 380
TseI GCWGC 3 cut(s) 628, 680, 839
Tsp45I GTSAC 2 cut(s) 200, 380
TspDTI ATGAA 5 cut(s) 91, 446, 555, 576, 725
TspGWI ACGGA 4 cut(s) 40, 281, 368, 1076
TspRI CASTG 3 cut(s) 303, 316, 849
VpaK11BI GGWCC 1 cut(s) 697
XapI RAATTY 1 cut(s) 866
XbaI TCTAGA 1 cut(s) 955
XmnI GAANNNNTTC 2 cut(s) 173, 453
XspI CTAG 3 cut(s) 956, 1047, 1354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.