Rh4DG399000

Clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0017660
Physical Location & Seq
Forward (+)
61540652 .. 61541476
825 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG399000.1

Sequence Viewer

Length: 765 bp
ATGGAAGAAATTAGTCGAATGCTTGAAATGTTGCCGCAGCTACAAAGCCTAACTGATCGAGTCATGGACTGCCGGCCAACTGGGCCGGCAGCTAAAAGCTTCTTGGTCCAAATGGCCATGAAACTTCTCATTCGAGACAGCTTTGTGTTTTACACAATCTTTAGGAGGGAAATTGTTATGGTTCTTGATAGCCTCTTTCAGATGCCTTATCGGAACTGCATATCAGCTTTTGAGATTTACAAGAAGGCAGCAGTGCAAGCAAATGACCTTTGTGAATTCTACGACTGGTGTAAGGCAATGGGATTGTGTGGTGCATATGAGTATCCATTTATCGATCGAATCCCACATATTCAAATCCATGCTCTAGAGAACTTCCTCCATGGAATGTGGCAGTTGACAGATCAGTCCTCTTCCACGCCGACCTCATCGTCATCCTCTTCTTTGGTGGAGTGCTCTAGTGAAACTGTAACGGAGGACGACAGCATTCTTGTTAGTACAAAATGGGAGAAGCCATTGATTCAGTTCGGTGGGGATCACGAGAACGAAAAGGCCTTAATTCTGTTTGGGAAAGATGCGGAGGAGAAGATGTTGATTCAGTTTGAAGAAGTGGAGGAGAGCTGGGAGAGCATTCTGGAAGCTTCCATCAATGTCTCACCTTCTTATCAACAAAACAATATGTTGTGCTTGTACAATCCGAACGTCGTTAATCCTTTCTATCACCATGGATTTGTTACAACCAACAACGCCATTCCTTCCTTAATCTAG

Protein Analysis

254

Amino Acids

29.12

Weight (kDa)

4.69

Isoelectric Point (pI)

55.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 4 - 127 1.8e-25 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017660)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g33220
malus_domestica MD13G1017400.v1.1 MD16G1015500.v1.1
prunus_persica Prupe.1G336100_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0442371
rosa_laevigata RLG00000006015
rosa_multiflora Rmu_co8473163.1_g000001 Rmu_sc0000124.1_g000044
rosa_rugosa Rorug04G0340600
rosa_samantha Rh4BG405900 Rh4CG421000 Rh4DG399000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 403, 427
AciI CCGC 2 cut(s) 35, 575
AclWI GGATC 1 cut(s) 540
AcoI YGGCCR 2 cut(s) 74, 114
AcsI RAATTY 1 cut(s) 275
AfaI GTAC 2 cut(s) 496, 689
AgsI TTSAA 3 cut(s) 26, 353, 602
AluBI AGCT 7 cut(s) 40, 92, 99, 141, 227, 618, 638
AluI AGCT 7 cut(s) 40, 92, 99, 141, 227, 618, 638
Alw21I GWGCWC 1 cut(s) 455
Alw26I GTCTC 2 cut(s) 129, 655
AlwI GGATC 1 cut(s) 540
AoxI GGCC 4 cut(s) 74, 83, 114, 549
ApeKI GCWGC 3 cut(s) 37, 89, 248
ApoI RAATTY 1 cut(s) 275
AspS9I GGNCC 2 cut(s) 83, 106
AsuHPI GGTGA 2 cut(s) 645, 710
AvaII GGWCC 1 cut(s) 106
BalI TGGCCA 1 cut(s) 116
BauI CACGAG 1 cut(s) 536
Bbv12I GWGCWC 1 cut(s) 455
BbvI GCAGC 3 cut(s) 49, 101, 260
BccI CCATC 1 cut(s) 650
BciVI GTATCC 1 cut(s) 333
BcoDI GTCTC 2 cut(s) 129, 655
BfaI CTAG 3 cut(s) 365, 456, 763
BfuI GTATCC 1 cut(s) 333
BglI GCCNNNNNGGC 1 cut(s) 82
BisI GCNGC 4 cut(s) 35, 38, 90, 249
BlsI GCNGC 4 cut(s) 36, 39, 91, 250
Bme18I GGWCC 1 cut(s) 106
BmgT120I GGNCC 2 cut(s) 83, 106
BmrI ACTGGG 1 cut(s) 90
BmsI GCATC 2 cut(s) 192, 562
BmuI ACTGGG 1 cut(s) 90
Bsa29I ATCGAT 1 cut(s) 333
BsaJI CCNNGG 2 cut(s) 379, 721
BsaXI ACNNNNNCTCC 2 cut(s) 572, 602
Bse118I RCCGGY 2 cut(s) 72, 85
Bse1I ACTGG 2 cut(s) 85, 290
Bse3DI GCAATG 1 cut(s) 303
BseCI ATCGAT 1 cut(s) 333
BseDI CCNNGG 2 cut(s) 379, 721
BseGI GGATG 1 cut(s) 431
BseMI GCAATG 1 cut(s) 303
BseNI ACTGG 2 cut(s) 85, 290
BseRI GAGGAG 2 cut(s) 593, 626
BseXI GCAGC 3 cut(s) 49, 101, 260
BseYI CCCAGC 1 cut(s) 618
Bsh1285I CGRYCG 1 cut(s) 337
BshFI GGCC 4 cut(s) 76, 85, 116, 551
BshVI ATCGAT 1 cut(s) 333
BsiEI CGRYCG 1 cut(s) 337
BsiHKAI GWGCWC 1 cut(s) 455
BsiSI CCGG 2 cut(s) 73, 86
BsmAI GTCTC 2 cut(s) 129, 655
BsmI GAATGC 3 cut(s) 24, 483, 627
BsnI GGCC 4 cut(s) 76, 85, 116, 551
Bsp1286I GDGCHC 1 cut(s) 455
Bsp1407I TGTACA 1 cut(s) 687
Bsp143I GATC 4 cut(s) 55, 334, 400, 532
Bsp19I CCATGG 2 cut(s) 379, 721
BspACI CCGC 2 cut(s) 35, 575
BspANI GGCC 4 cut(s) 76, 85, 116, 551
BspDI ATCGAT 1 cut(s) 333
BspPI GGATC 1 cut(s) 540
BsrDI GCAATG 1 cut(s) 303
BsrFI RCCGGY 2 cut(s) 72, 85
BsrGI TGTACA 1 cut(s) 687
BsrI ACTGG 2 cut(s) 85, 290
BssAI RCCGGY 2 cut(s) 72, 85
BssECI CCNNGG 2 cut(s) 379, 721
BssMI GATC 4 cut(s) 55, 334, 400, 532
BssSI CACGAG 1 cut(s) 536
BssT1I CCWWGG 2 cut(s) 379, 721
Bst2BI CACGAG 1 cut(s) 536
Bst4CI ACNGT 1 cut(s) 466
Bst6I CTCTTC 2 cut(s) 415, 442
BstAUI TGTACA 1 cut(s) 687
BstC8I GCNNGC 3 cut(s) 74, 87, 258
BstDSI CCRYGG 2 cut(s) 379, 721
BstF5I GGATG 1 cut(s) 431
BstKTI GATC 4 cut(s) 58, 337, 403, 535
BstMAI GTCTC 2 cut(s) 129, 655
BstMBI GATC 4 cut(s) 55, 334, 400, 532
BstMCI CGRYCG 1 cut(s) 337
BstMWI GCNNNNNNNGC 3 cut(s) 82, 257, 624
BstV1I GCAGC 3 cut(s) 49, 101, 260
Bsu15I ATCGAT 1 cut(s) 333
BsuI GTATCC 1 cut(s) 333
BsuRI GGCC 4 cut(s) 76, 85, 116, 551
BsuTUI ATCGAT 1 cut(s) 333
BtgI CCRYGG 2 cut(s) 379, 721
BtsCI GGATG 1 cut(s) 431
BtsI GCAGTG 1 cut(s) 258
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 3 cut(s) 74, 87, 258
Cfr10I RCCGGY 2 cut(s) 72, 85
Cfr13I GGNCC 2 cut(s) 83, 106
ClaI ATCGAT 1 cut(s) 333
Csp6I GTAC 2 cut(s) 495, 688
CviAII CATG 5 cut(s) 64, 118, 359, 380, 722
CviQI GTAC 2 cut(s) 495, 688
DpnI GATC 4 cut(s) 57, 336, 402, 534
DpnII GATC 4 cut(s) 55, 334, 400, 532
DrdI GACNNNNNNGTC 2 cut(s) 403, 427
DseDI GACNNNNNNGTC 2 cut(s) 403, 427
EaeI YGGCCR 2 cut(s) 74, 114
Eam1104I CTCTTC 2 cut(s) 415, 442
EarI CTCTTC 2 cut(s) 415, 442
Eco130I CCWWGG 2 cut(s) 379, 721
Eco147I AGGCCT 1 cut(s) 551
Eco47I GGWCC 1 cut(s) 106
EcoRI GAATTC 1 cut(s) 275
EcoT14I CCWWGG 2 cut(s) 379, 721
ErhI CCWWGG 2 cut(s) 379, 721
FaeI CATG 5 cut(s) 67, 121, 362, 383, 725
FatI CATG 5 cut(s) 63, 117, 358, 379, 721
FauNDI CATATG 1 cut(s) 316
Fnu4HI GCNGC 4 cut(s) 35, 38, 90, 249
FokI GGATG 1 cut(s) 418
Fsp4HI GCNGC 4 cut(s) 35, 38, 90, 249
FspBI CTAG 3 cut(s) 365, 456, 763
GluI GCNGC 4 cut(s) 35, 38, 90, 249
GsaI CCCAGC 1 cut(s) 622
HaeIII GGCC 4 cut(s) 76, 85, 116, 551
HapII CCGG 2 cut(s) 73, 86
Hin1II CATG 5 cut(s) 67, 121, 362, 383, 725
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HindIII AAGCTT 2 cut(s) 97, 636
HinfI GANTC 4 cut(s) 60, 339, 517, 592
HpaII CCGG 2 cut(s) 73, 86
HphI GGTGA 2 cut(s) 645, 710
Hpy166II GTNNAC 1 cut(s) 396
Hpy188I TCNGA 3 cut(s) 201, 213, 696
Hpy188III TCNNGA 5 cut(s) 134, 185, 365, 536, 632
Hpy8I GTNNAC 1 cut(s) 396
Hpy99I CGWCG 1 cut(s) 704
HpyAV CCTTC 3 cut(s) 238, 666, 762
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4IV ACGT 1 cut(s) 699
HpyCH4V TGCA 3 cut(s) 219, 256, 314
HpyF10VI GCNNNNNNNGC 3 cut(s) 82, 257, 624
HpySE526I ACGT 1 cut(s) 699
Hsp92II CATG 5 cut(s) 67, 121, 362, 383, 725
KroI GCCGGC 2 cut(s) 72, 85
KroNI GCCGGC 2 cut(s) 74, 87
Kzo9I GATC 4 cut(s) 55, 334, 400, 532
LpnPI CCDG 6 cut(s) 66, 86, 99, 271, 604, 617
Lsp1109I GCAGC 3 cut(s) 49, 101, 260
LweI GCATC 2 cut(s) 192, 562
MaeI CTAG 3 cut(s) 365, 456, 763
MaeII ACGT 1 cut(s) 699
MaeIII GTNAC 2 cut(s) 466, 730
MalI GATC 4 cut(s) 57, 336, 402, 534
MboI GATC 4 cut(s) 55, 334, 400, 532
MboII GAAGA 5 cut(s) 17, 402, 429, 595, 614
MhlI GDGCHC 1 cut(s) 455
MlsI TGGCCA 1 cut(s) 116
MluCI AATT 4 cut(s) 9, 171, 275, 555
MluNI TGGCCA 1 cut(s) 116
MlyI GAGTC 1 cut(s) 69
MnlI CCTC 9 cut(s) 159, 203, 386, 418, 433, 445, 466, 571, 604
Mox20I TGGCCA 1 cut(s) 116
MroNI GCCGGC 2 cut(s) 72, 85
MscI TGGCCA 1 cut(s) 116
MseI TTAA 3 cut(s) 554, 705, 758
Msp20I TGGCCA 1 cut(s) 116
MspI CCGG 2 cut(s) 73, 86
Mva1269I GAATGC 3 cut(s) 24, 483, 627
MwoI GCNNNNNNNGC 3 cut(s) 82, 257, 624
NaeI GCCGGC 2 cut(s) 74, 87
NcoI CCATGG 2 cut(s) 379, 721
NdeI CATATG 1 cut(s) 316
NdeII GATC 4 cut(s) 55, 334, 400, 532
NgoMIV GCCGGC 2 cut(s) 72, 85
NlaIII CATG 5 cut(s) 67, 121, 362, 383, 725
PceI AGGCCT 1 cut(s) 551
PctI GAATGC 3 cut(s) 24, 483, 627
PdiI GCCGGC 2 cut(s) 74, 87
PfeI GAWTC 3 cut(s) 339, 517, 592
PkrI GCNGC 4 cut(s) 36, 39, 91, 250
Ple19I CGATCG 1 cut(s) 337
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
PspFI CCCAGC 1 cut(s) 618
PspPI GGNCC 2 cut(s) 83, 106
PvuI CGATCG 1 cut(s) 337
RsaI GTAC 2 cut(s) 496, 689
RsaNI GTAC 2 cut(s) 495, 688
SaqAI TTAA 3 cut(s) 554, 705, 758
SatI GCNGC 4 cut(s) 35, 38, 90, 249
Sau3AI GATC 4 cut(s) 55, 334, 400, 532
Sau96I GGNCC 2 cut(s) 83, 106
SchI GAGTC 1 cut(s) 69
SduI GDGCHC 1 cut(s) 455
SfaNI GCATC 2 cut(s) 192, 562
SfiI GGCCNNNNNGGCC 1 cut(s) 82
SinI GGWCC 1 cut(s) 106
Sse9I AATT 4 cut(s) 9, 171, 275, 555
SseBI AGGCCT 1 cut(s) 551
SsiI CCGC 2 cut(s) 35, 575
SspMI CTAG 3 cut(s) 365, 456, 763
StuI AGGCCT 1 cut(s) 551
StyI CCWWGG 2 cut(s) 379, 721
TaaI ACNGT 1 cut(s) 466
TaiI ACGT 1 cut(s) 702
TaqI TCGA 5 cut(s) 16, 58, 133, 333, 337
TasI AATT 4 cut(s) 9, 171, 275, 555
TatI WGTACW 2 cut(s) 494, 687
TauI GCSGC 1 cut(s) 37
TfiI GAWTC 3 cut(s) 339, 517, 592
Tru1I TTAA 3 cut(s) 554, 705, 758
Tru9I TTAA 3 cut(s) 554, 705, 758
TscAI CASTG 1 cut(s) 258
TseI GCWGC 3 cut(s) 37, 89, 248
TspDTI ATGAA 1 cut(s) 134
TspGWI ACGGA 1 cut(s) 485
TspRI CASTG 1 cut(s) 258
VpaK11BI GGWCC 1 cut(s) 106
XapI RAATTY 1 cut(s) 275
XbaI TCTAGA 1 cut(s) 364
XspI CTAG 3 cut(s) 365, 456, 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.