MD16G1015500.v1.1

Clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0017660
Physical Location & Seq
Reverse (-)
1203334 .. 1204779
1446 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1015500.v1.1.491

Sequence Viewer

Length: 1446 bp
ATGCAGAAGCGATTTCGGCAAGTTTTCACAGCTCTAAGAGAGCACAGCTCCGTGAACTATGCCAAAATCGCTATGCTTGGAGGGTTTTGCAATGTCGAACTCATCATCATAAAAGCCACTGCTCCTGATGACTTGCCATTGCCCGAAAAGTACATTCAGGAGCTCTTGAAAATCTTCTCCATTTCCCCGTCTTCGTTAAGAGATTTTTTACTCAGCTTCGCTCGCCGCTTTGGCAAGACACACTGCTGGCGGGTTGCACTCAAGTGTCTAATTCTACTCCACCGCCTACTCCGTTCGGTGCCTGAAGACAGCAACTTCCGGTCTGAGCTCCTTTGGGCTCAATCCAACGGTTTGATGTCTCTCAATCAGTGTCACTTCCGCGATGATTCTTCCTTGGCTTCTGCCTATTACAGTGCCCTTATTAGATCGTACGCTCAGCTCCTCGACGAAGCTCTTCATTGCTATTGGTTGGATTCCAAGGCAGCCTACGACCAGCAAGAACAACGACAATATGAAGAATATCGCGAACAAGAAGAAGAATACGAAGAAGACGGAAAAGAAGAAGAAGAGCCGGAACTTGAGAGCTTATCCAATAGAATGACGGAAGTAGGTCGAATGCTTGAAGTGTTGCCGCAGCTGCAAACCCTCATTGACCTTGTCATGGACTGCCGTCCCACAGGACCAGCAGCCAAAGCCTTCCTCGTCCAACTCGCCATGAAACACATCATCAGAGACAGCCTCATGTGCTACAGAATCTTCTGCAGCGAAATCGTCACGGTTCTGGACAGTTTGTTTCAAATGCCATACAGGAGTTGCATTTTGGCTTTCGGCATTTACAAAAAGGCAGCCGTCCAAGCAAACCAACTCAGCGAATTTTACGAGTGGTGTAAAGCCATGGGACTCTGCGGATCGTACGAGTATCCCTTCGTTGATCAAATCCCACACATACAAATTCACGCTCTCGAGAATTTTCTCAACGGTATGTGGCAGTTGACGGAGTCCTCATCCACACCAACTTCGCCAATTTCTTCGGCCTCGGTGCCGTCATCTTTCGTGGAGTTTTCAAGTTCCAGCACTTTGACAGAGGACGATATTAAGGATTTTGTATTTATGTCACAAAAGGAGCATATTCTTGTTAGTACAAAATGGGAGAAGTCGTTGATTCAGTTCGAAAGAGAAGGTTACAACGAGGAGAAGCTGTTGATTCAGTTTGATGAGGAGAAGCCGTTGATTGAATTCGAAGATGATATCGTTGACGAAAGCTGGGAAAGCCTGCTTGATGCTTCCATTAACAAGTCTCCTGCTGCTGAAACTCAACAAAACAATGTATGGCAGATGCAAATATACAACCCCAATGCTCTAAATCCTTTCCATGATTCCATCAGCCAGTCATCGGGCCAAATTACCATGGGGACTATAACGTTTGCTGTGCCCAAATTTAGATGA

Protein Analysis

482

Amino Acids

55.49

Weight (kDa)

4.87

Isoelectric Point (pI)

57.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 35 - 326 3.6e-47 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017660)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g33220
malus_domestica MD13G1017400.v1.1 MD16G1015500.v1.1
prunus_persica Prupe.1G336100_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0442371
rosa_laevigata RLG00000006015
rosa_multiflora Rmu_co8473163.1_g000001 Rmu_sc0000124.1_g000044
rosa_rugosa Rorug04G0340600
rosa_samantha Rh4BG405900 Rh4CG421000 Rh4DG399000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 298, 1039
AccII CGCG 2 cut(s) 381, 525
AciI CCGC 6 cut(s) 226, 250, 283, 379, 632, 906
AclI AACGTT 1 cut(s) 1421
AclWI GGATC 1 cut(s) 916
AcsI RAATTY 5 cut(s) 872, 951, 967, 1235, 1436
AcuI CTGAAG 1 cut(s) 324
AfaI GTAC 4 cut(s) 152, 431, 914, 1141
AfiI CCNNNNNNNGG 2 cut(s) 661, 1393
AgsI TTSAA 5 cut(s) 169, 623, 797, 1065, 1235
AleI CACNNNNGTG 1 cut(s) 262
Alw21I GWGCWC 3 cut(s) 45, 165, 330
Alw26I GTCTC 3 cut(s) 363, 726, 1302
AlwI GGATC 1 cut(s) 916
Ama87I CYCGRG 1 cut(s) 962
AoxI GGCC 2 cut(s) 1032, 1396
ApeKI GCWGC 7 cut(s) 482, 634, 637, 686, 762, 845, 1304
ApoI RAATTY 5 cut(s) 872, 951, 967, 1235, 1436
Asp700I GAANNNNTTC 2 cut(s) 173, 453
AspS9I GGNCC 2 cut(s) 680, 1396
AsuII TTCGAA 2 cut(s) 1170, 1239
AvaI CYCGRG 1 cut(s) 962
AvaII GGWCC 1 cut(s) 680
BaeGI GKGCMC 2 cut(s) 418, 1434
BanI GGYRCC 2 cut(s) 298, 1039
BanII GRGCYC 3 cut(s) 165, 330, 340
BbsI GAAGAC 3 cut(s) 183, 312, 555
Bbv12I GWGCWC 3 cut(s) 45, 165, 330
BbvI GCAGC 7 cut(s) 494, 624, 646, 698, 774, 857, 1291
BccI CCATC 1 cut(s) 1388
BceAI ACGGC 4 cut(s) 654, 833, 1027, 1210
BcgI CGANNNNNNTGC 2 cut(s) 751, 785
BciVI GTATCC 1 cut(s) 930
BclI TGATCA 1 cut(s) 931
BcoDI GTCTC 3 cut(s) 363, 726, 1302
BfmI CTRYAG 2 cut(s) 748, 760
BfuI GTATCC 1 cut(s) 930
BglI GCCNNNNNGGC 1 cut(s) 231
BisI GCNGC 9 cut(s) 226, 483, 632, 635, 638, 687, 763, 846, 1305
BlpI GCTNAGC 1 cut(s) 435
BlsI GCNGC 9 cut(s) 227, 484, 633, 636, 639, 688, 764, 847, 1306
Bme18I GGWCC 1 cut(s) 680
BmeT110I CYCGRG 1 cut(s) 962
BmgT120I GGNCC 2 cut(s) 680, 1396
BmiI GGNNCC 2 cut(s) 300, 1041
BmsI GCATC 2 cut(s) 1270, 1326
BoxI GACNNNNGTC 1 cut(s) 669
BpiI GAAGAC 3 cut(s) 183, 312, 555
BplI GAGNNNNNCTC 4 cut(s) 32, 64, 723, 755
Bpu1102I GCTNAGC 1 cut(s) 435
Bpu14I TTCGAA 2 cut(s) 1170, 1239
BpuEI CTTGAG 2 cut(s) 245, 599
BsaJI CCNNGG 5 cut(s) 393, 477, 894, 1035, 1407
BsaWI WCCGGW 1 cut(s) 318
BsaXI ACNNNNNCTCC 6 cut(s) 1142, 1172, 1184, 1211, 1214, 1241
Bsc4I CCNNNNNNNGG 2 cut(s) 661, 1393
Bse1I ACTGG 1 cut(s) 1387
Bse3DI GCAATG 3 cut(s) 97, 137, 457
BseDI CCNNGG 5 cut(s) 393, 477, 894, 1035, 1407
BseGI GGATG 1 cut(s) 1004
BseLI CCNNNNNNNGG 2 cut(s) 661, 1393
BseMI GCAATG 3 cut(s) 97, 137, 457
BseMII CTCAG 4 cut(s) 226, 315, 449, 880
BseNI ACTGG 1 cut(s) 1387
BseRI GAGGAG 3 cut(s) 431, 1205, 1232
BseSI GKGCMC 2 cut(s) 418, 1434
BseXI GCAGC 7 cut(s) 494, 624, 646, 698, 774, 857, 1291
BseYI CCCAGC 1 cut(s) 1263
Bsh1236I CGCG 2 cut(s) 381, 525
BshFI GGCC 2 cut(s) 1034, 1398
BshNI GGYRCC 2 cut(s) 298, 1039
BsiHKAI GWGCWC 3 cut(s) 45, 165, 330
BsiHKCI CYCGRG 1 cut(s) 962
BsiSI CCGG 2 cut(s) 319, 572
BsiWI CGTACG 2 cut(s) 429, 912
BslFI GGGAC 3 cut(s) 657, 912, 1426
BslI CCNNNNNNNGG 2 cut(s) 661, 1393
BsmAI GTCTC 3 cut(s) 363, 726, 1302
BsmFI GGGAC 3 cut(s) 657, 912, 1426
BsmI GAATGC 1 cut(s) 621
BsnI GGCC 2 cut(s) 1034, 1398
BsoBI CYCGRG 1 cut(s) 962
Bsp119I TTCGAA 2 cut(s) 1170, 1239
Bsp1286I GDGCHC 6 cut(s) 45, 165, 330, 340, 418, 1434
Bsp143I GATC 3 cut(s) 425, 908, 931
Bsp1720I GCTNAGC 1 cut(s) 435
Bsp19I CCATGG 2 cut(s) 894, 1407
Bsp68I TCGCGA 1 cut(s) 525
BspACI CCGC 6 cut(s) 226, 250, 283, 379, 632, 906
BspANI GGCC 2 cut(s) 1034, 1398
BspCNI CTCAG 4 cut(s) 225, 316, 448, 879
BspFNI CGCG 2 cut(s) 381, 525
BspLI GGNNCC 2 cut(s) 300, 1041
BspMAI CTGCAG 1 cut(s) 764
BspPI GGATC 1 cut(s) 916
BspQI GCTCTTC 2 cut(s) 459, 561
BspT104I TTCGAA 2 cut(s) 1170, 1239
BspT107I GGYRCC 2 cut(s) 298, 1039
BsrDI GCAATG 3 cut(s) 97, 137, 457
BsrI ACTGG 1 cut(s) 1387
BssECI CCNNGG 5 cut(s) 393, 477, 894, 1035, 1407
BssMI GATC 3 cut(s) 425, 908, 931
BssT1I CCWWGG 4 cut(s) 393, 477, 894, 1407
Bst4CI ACNGT 5 cut(s) 350, 413, 778, 788, 980
Bst6I CTCTTC 2 cut(s) 459, 561
BstBI TTCGAA 2 cut(s) 1170, 1239
BstC8I GCNNGC 3 cut(s) 223, 248, 1274
BstDEI CTNAG 5 cut(s) 35, 212, 324, 435, 866
BstDSI CCRYGG 2 cut(s) 894, 1407
BstF5I GGATG 1 cut(s) 1004
BstFNI CGCG 2 cut(s) 381, 525
BstKTI GATC 3 cut(s) 428, 911, 934
BstMAI GTCTC 3 cut(s) 363, 726, 1302
BstMBI GATC 3 cut(s) 425, 908, 931
BstMWI GCNNNNNNNGC 9 cut(s) 16, 68, 222, 231, 637, 692, 744, 854, 1269
BstPAI GACNNNNGTC 1 cut(s) 669
BstSFI CTRYAG 2 cut(s) 748, 760
BstSLI GKGCMC 2 cut(s) 418, 1434
BstUI CGCG 2 cut(s) 381, 525
BstV1I GCAGC 7 cut(s) 494, 624, 646, 698, 774, 857, 1291
BstV2I GAAGAC 3 cut(s) 183, 312, 555
BsuI GTATCC 1 cut(s) 930
BsuRI GGCC 2 cut(s) 1034, 1398
BtgI CCRYGG 2 cut(s) 894, 1407
BtgZI GCGATG 1 cut(s) 396
BtsCI GGATG 1 cut(s) 1004
BtsI GCAGTG 2 cut(s) 117, 241
BtsIMutI CAGTG 4 cut(s) 117, 241, 374, 418
BtuMI TCGCGA 1 cut(s) 525
Cac8I GCNNGC 3 cut(s) 223, 248, 1274
Cfr13I GGNCC 2 cut(s) 680, 1396
Csp6I GTAC 4 cut(s) 151, 430, 913, 1140
CviAII CATG 6 cut(s) 661, 715, 742, 895, 1373, 1408
CviQI GTAC 4 cut(s) 151, 430, 913, 1140
DdeI CTNAG 5 cut(s) 35, 212, 324, 435, 866
DpnI GATC 3 cut(s) 427, 910, 933
DpnII GATC 3 cut(s) 425, 908, 931
Eam1104I CTCTTC 2 cut(s) 459, 561
EarI CTCTTC 2 cut(s) 459, 561
Ecl136II GAGCTC 2 cut(s) 163, 328
Eco130I CCWWGG 4 cut(s) 393, 477, 894, 1407
Eco24I GRGCYC 3 cut(s) 165, 330, 340
Eco32I GATATC 1 cut(s) 1249
Eco47I GGWCC 1 cut(s) 680
Eco53kI GAGCTC 2 cut(s) 163, 328
Eco57I CTGAAG 1 cut(s) 324
Eco88I CYCGRG 1 cut(s) 962
EcoICRI GAGCTC 2 cut(s) 163, 328
EcoRI GAATTC 1 cut(s) 1235
EcoRV GATATC 1 cut(s) 1249
EcoT14I CCWWGG 4 cut(s) 393, 477, 894, 1407
EcoT38I GRGCYC 3 cut(s) 165, 330, 340
ErhI CCWWGG 4 cut(s) 393, 477, 894, 1407
FaeI CATG 6 cut(s) 664, 718, 745, 898, 1376, 1411
FaqI GGGAC 3 cut(s) 657, 912, 1426
FatI CATG 6 cut(s) 660, 714, 741, 894, 1372, 1407
FauI CCCGC 1 cut(s) 243
FbaI TGATCA 1 cut(s) 931
Fnu4HI GCNGC 9 cut(s) 226, 483, 632, 635, 638, 687, 763, 846, 1305
FokI GGATG 1 cut(s) 991
FriOI GRGCYC 3 cut(s) 165, 330, 340
Fsp4HI GCNGC 9 cut(s) 226, 483, 632, 635, 638, 687, 763, 846, 1305
GluI GCNGC 9 cut(s) 226, 483, 632, 635, 638, 687, 763, 846, 1305
GsaI CCCAGC 1 cut(s) 1267
HaeIII GGCC 2 cut(s) 1034, 1398
HapII CCGG 2 cut(s) 319, 572
Hin1II CATG 6 cut(s) 664, 718, 745, 898, 1376, 1411
HincII GTYRAC 2 cut(s) 993, 1255
HindII GTYRAC 2 cut(s) 993, 1255
HinfI GANTC 8 cut(s) 386, 473, 753, 900, 998, 1162, 1204, 1376
HpaII CCGG 2 cut(s) 319, 572
Hpy166II GTNNAC 3 cut(s) 55, 993, 1255
Hpy188I TCNGA 2 cut(s) 325, 731
Hpy188III TCNNGA 7 cut(s) 125, 158, 166, 524, 782, 962, 964
Hpy8I GTNNAC 3 cut(s) 55, 993, 1255
Hpy99I CGWCG 1 cut(s) 449
HpyAV CCTTC 3 cut(s) 706, 934, 1172
HpyCH4III ACNGT 5 cut(s) 350, 413, 778, 788, 980
HpyCH4IV ACGT 1 cut(s) 1421
HpyCH4V TGCA 7 cut(s) 4, 90, 257, 640, 762, 816, 1339
HpyF10VI GCNNNNNNNGC 9 cut(s) 16, 68, 222, 231, 637, 692, 744, 854, 1269
HpyF3I CTNAG 5 cut(s) 35, 212, 324, 435, 866
HpySE526I ACGT 1 cut(s) 1421
Hsp92II CATG 6 cut(s) 664, 718, 745, 898, 1376, 1411
Ksp22I TGATCA 1 cut(s) 931
Kzo9I GATC 3 cut(s) 425, 908, 931
LguI GCTCTTC 2 cut(s) 459, 561
LmnI GCTCC 6 cut(s) 53, 127, 160, 333, 444, 1123
Lsp1109I GCAGC 7 cut(s) 494, 624, 646, 698, 774, 857, 1291
LweI GCATC 2 cut(s) 1270, 1326
MaeII ACGT 1 cut(s) 1421
MaeIII GTNAC 4 cut(s) 371, 772, 1113, 1181
MalI GATC 3 cut(s) 427, 910, 933
MboI GATC 3 cut(s) 425, 908, 931
MhlI GDGCHC 6 cut(s) 45, 165, 330, 340, 418, 1434
MluCI AATT 8 cut(s) 270, 872, 951, 967, 1023, 1235, 1401, 1436
MlyI GAGTC 2 cut(s) 894, 1007
MmeI TCCRAC 3 cut(s) 369, 450, 730
MroXI GAANNNNTTC 2 cut(s) 173, 453
MseI TTAA 3 cut(s) 197, 1095, 1290
MslI CAYNNNNRTG 1 cut(s) 262
MspA1I CMGCKG 1 cut(s) 637
MspI CCGG 2 cut(s) 319, 572
Mva1269I GAATGC 1 cut(s) 621
MvnI CGCG 2 cut(s) 381, 525
MwoI GCNNNNNNNGC 9 cut(s) 16, 68, 222, 231, 637, 692, 744, 854, 1269
NcoI CCATGG 2 cut(s) 894, 1407
NdeII GATC 3 cut(s) 425, 908, 931
NlaIII CATG 6 cut(s) 664, 718, 745, 898, 1376, 1411
NlaIV GGNNCC 2 cut(s) 300, 1041
NmuCI GTSAC 3 cut(s) 371, 772, 1113
NruI TCGCGA 1 cut(s) 525
NspV TTCGAA 2 cut(s) 1170, 1239
OliI CACNNNNGTG 1 cut(s) 262
PaeR7I CTCGAG 1 cut(s) 962
PciSI GCTCTTC 2 cut(s) 459, 561
PctI GAATGC 1 cut(s) 621
PdmI GAANNNNTTC 2 cut(s) 173, 453
PfeI GAWTC 6 cut(s) 386, 473, 753, 1162, 1204, 1376
Pfl23II CGTACG 2 cut(s) 429, 912
PflFI GACNNNGTC 2 cut(s) 656, 997
PkrI GCNGC 9 cut(s) 227, 484, 633, 636, 639, 688, 764, 847, 1306
PleI GAGTC 2 cut(s) 894, 1006
PpsI GAGTC 2 cut(s) 894, 1006
PshAI GACNNNNGTC 1 cut(s) 669
Psp124BI GAGCTC 2 cut(s) 165, 330
Psp1406I AACGTT 1 cut(s) 1421
PspFI CCCAGC 1 cut(s) 1263
PspLI CGTACG 2 cut(s) 429, 912
PspN4I GGNNCC 2 cut(s) 300, 1041
PspPI GGNCC 2 cut(s) 680, 1396
PstI CTGCAG 1 cut(s) 764
PsyI GACNNNGTC 2 cut(s) 656, 997
PvuII CAGCTG 1 cut(s) 637
RruI TCGCGA 1 cut(s) 525
RsaI GTAC 4 cut(s) 152, 431, 914, 1141
RsaNI GTAC 4 cut(s) 151, 430, 913, 1140
RseI CAYNNNNRTG 1 cut(s) 262
SacI GAGCTC 2 cut(s) 165, 330
SapI GCTCTTC 2 cut(s) 459, 561
SaqAI TTAA 3 cut(s) 197, 1095, 1290
SatI GCNGC 9 cut(s) 226, 483, 632, 635, 638, 687, 763, 846, 1305
Sau3AI GATC 3 cut(s) 425, 908, 931
Sau96I GGNCC 2 cut(s) 680, 1396
SchI GAGTC 2 cut(s) 894, 1007
SduI GDGCHC 6 cut(s) 45, 165, 330, 340, 418, 1434
SfaNI GCATC 2 cut(s) 1270, 1326
SfcI CTRYAG 2 cut(s) 748, 760
Sfr274I CTCGAG 1 cut(s) 962
SfuI TTCGAA 2 cut(s) 1170, 1239
SinI GGWCC 1 cut(s) 680
SlaI CTCGAG 1 cut(s) 962
SmiMI CAYNNNNRTG 1 cut(s) 262
SmlI CTYRAG 3 cut(s) 260, 578, 962
SmoI CTYRAG 3 cut(s) 260, 578, 962
Sse9I AATT 8 cut(s) 270, 872, 951, 967, 1023, 1235, 1401, 1436
SsiI CCGC 6 cut(s) 226, 250, 283, 379, 632, 906
SstI GAGCTC 2 cut(s) 165, 330
StyI CCWWGG 4 cut(s) 393, 477, 894, 1407
TaaI ACNGT 5 cut(s) 350, 413, 778, 788, 980
TaiI ACGT 1 cut(s) 1424
TaqI TCGA 6 cut(s) 96, 444, 613, 963, 1170, 1239
TasI AATT 8 cut(s) 270, 872, 951, 967, 1023, 1235, 1401, 1436
TatI WGTACW 2 cut(s) 150, 1139
TauI GCSGC 2 cut(s) 228, 634
TfiI GAWTC 6 cut(s) 386, 473, 753, 1162, 1204, 1376
Tru1I TTAA 3 cut(s) 197, 1095, 1290
Tru9I TTAA 3 cut(s) 197, 1095, 1290
TscAI CASTG 4 cut(s) 124, 248, 374, 418
TseFI GTSAC 3 cut(s) 371, 772, 1113
TseI GCWGC 7 cut(s) 482, 634, 637, 686, 762, 845, 1304
Tsp45I GTSAC 3 cut(s) 371, 772, 1113
TspDTI ATGAA 3 cut(s) 446, 528, 731
TspGWI ACGGA 5 cut(s) 40, 281, 567, 617, 1010
TspRI CASTG 4 cut(s) 124, 248, 374, 418
Tth111I GACNNNGTC 2 cut(s) 656, 997
VpaK11BI GGWCC 1 cut(s) 680
XapI RAATTY 5 cut(s) 872, 951, 967, 1235, 1436
XhoI CTCGAG 1 cut(s) 962
XmnI GAANNNNTTC 2 cut(s) 173, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.