MD13G1017400.v1.1

Clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0017660
Physical Location & Seq
Reverse (-)
1136957 .. 1138462
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1017400.v1.1.491

Sequence Viewer

Length: 1506 bp
ATGCAGCGGCGACTTCGGCAAGTTTTCAGTGCTCTAAGAGAGCACAGCTCCGTGAGCTATGCCAAGATTGCCACGGTTGGAGGGTTTTGCAATGTCGAACTCATCATTATAAAAGCAACGGCTCCTGATGACTTGCCGTTGCCCGAAAGGTACGTTCAGGAGCTCTTGAAAATCTTCTCCATCTCCCCATCTTCGTTGAGAGATTTTTCACTCAGCTTCAATCGCCGCTTTGTCAAGACTCGCTGCTGGCGGGTTGCGCTCAAGTGTCTAATTCTCCTCCACCGCCTACTCCGTTCGGTGCCTGAAGACAGCAACTTCCGTTCTGAGCTCCTTTGGACGCGATCCAACGGTTTGATGTCTCTCAATCAGTGTCACTTCCGCGATGATTCTTCCTCCGCTTCTAAAGATTACACAGCCTTTATTAGATCATACGCTTGCCTCCTCGACGAAGCACTTCATTGCTTTTGGTTGGATAGCAAGCCAGCGTATGACCAGCAAGATCAACAACAATACGAAGAATATCAATATCGCGAACAAGACGAAGAGTACAAAGAAGAAGGAGAAGAAGAAGAGCCGCAACTCGAGAGCTTATCGAATAGAATGACGGAAGTAGGCCGAATGCTTGAAGTGTTGCCGCACCTGCAAACCCTCATTGACCTTGTCATGGACTGCCGTCCCACAGGACCAGCAGCCAAAGCCTTCCTCGTCCAACTCGCTATGAAACACATCATAAGAGAAAGCTTCATGTGCTACACAATCTTCCGCCGTGAAATCGTCACGGTTCTGGACAGTTTGTTACAAATGCCATACAGTAGTTGCATCTCGGCTTTCGGCATTTACAAAAAGGCAGCCGTCCAAGCAAACCAACTCTCTGAATTCTACGAGTGGTGTAAAGCCATGGGATTCTACGGAGGGTACGAGTATCCCTTTATTGATCAAATCCCACACATACAAATTCATGCTCTCGAGAAATTCCTCAATGGTATGTGGCAGTTGACGGAGTCCTCATCGACACCAACTTCGCCAACTTCTTCGGCATCAGTGCCGTCATCTTTTGTGCAGTTTTCAAGTTCCACCACTTTGACAGAGGACGATATTGGTGACAGAGTATTTCTGTCACAAAAGGATCATATCCTTGTTAGTACAAAATGGGAGAAGCCGTTGATTCGGTTCGAGAGAGAAAGTTACGACGAGGAGAAGCTGTTAATTCAGTTGGACGAGGAGAAGCCGTTGATTCAGTTCGAAATAGAAGTTTTCGACAAGGAGAAGCCGTTAATTCAGTTTGACGAAGAAAAGCCGTTGATTGATTTCGAAGATGATATCGACAACGAGAGCTGGGAGAGCCTGCTTGAAGCTTCCATTAACAAGTCTCCAGCTGCTCAAACTCAACAAAACATTTCATGGCAGATGCAAATATACAACCCCAATGCTCTAAATCCATTCCATCAACCAGTCATCAGGCCAAATTACCATGGGGACTATAACGTTTGGTGTGCTCAAATTTAG

Protein Analysis

502

Amino Acids

58.33

Weight (kDa)

4.96

Isoelectric Point (pI)

58.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 35 - 327 1.1e-44 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017660)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g33220
malus_domestica MD13G1017400.v1.1 MD16G1015500.v1.1
prunus_persica Prupe.1G336100_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0442371
rosa_laevigata RLG00000006015
rosa_multiflora Rmu_co8473163.1_g000001 Rmu_sc0000124.1_g000044
rosa_rugosa Rorug04G0340600
rosa_samantha Rh4BG405900 Rh4CG421000 Rh4DG399000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 110
AarI CACCTGC 1 cut(s) 648
Acc36I ACCTGC 1 cut(s) 648
AccB1I GGYRCC 1 cut(s) 298
AccII CGCG 3 cut(s) 340, 381, 531
AciI CCGC 9 cut(s) 7, 226, 250, 283, 379, 396, 575, 635, 763
AclI AACGTT 1 cut(s) 1485
AclWI GGATC 2 cut(s) 336, 1134
AcsI RAATTY 4 cut(s) 875, 954, 971, 1500
AcuI CTGAAG 1 cut(s) 324
AfaI GTAC 4 cut(s) 152, 548, 917, 1144
AfiI CCNNNNNNNGG 1 cut(s) 664
AgsI TTSAA 5 cut(s) 169, 220, 626, 1068, 1352
Alw21I GWGCWC 5 cut(s) 34, 45, 165, 330, 1498
Alw26I GTCTC 2 cut(s) 363, 1374
AlwI GGATC 2 cut(s) 336, 1134
Ama87I CYCGRG 2 cut(s) 581, 965
AoxI GGCC 2 cut(s) 613, 1460
ApeKI GCWGC 5 cut(s) 4, 243, 689, 848, 1376
ApoI RAATTY 4 cut(s) 875, 954, 971, 1500
ArsI GACNNNNNNTTYG 2 cut(s) 1003, 1035
Asp700I GAANNNNTTC 2 cut(s) 173, 453
AspLEI GCGC 1 cut(s) 259
AspS9I GGNCC 1 cut(s) 683
AsuHPI GGTGA 1 cut(s) 1112
AsuII TTCGAA 2 cut(s) 1242, 1311
AvaI CYCGRG 2 cut(s) 581, 965
AvaII GGWCC 1 cut(s) 683
BanI GGYRCC 1 cut(s) 298
BanII GRGCYC 2 cut(s) 165, 330
BbsI GAAGAC 1 cut(s) 312
Bbv12I GWGCWC 5 cut(s) 34, 45, 165, 330, 1498
BbvI GCAGC 5 cut(s) 16, 230, 701, 860, 1363
BccI CCATC 3 cut(s) 188, 196, 1452
BciVI GTATCC 1 cut(s) 933
BclI TGATCA 1 cut(s) 934
BcoDI GTCTC 2 cut(s) 363, 1374
BfuAI ACCTGC 1 cut(s) 648
BfuI GTATCC 1 cut(s) 933
BisI GCNGC 9 cut(s) 5, 8, 226, 244, 575, 635, 690, 849, 1377
BlsI GCNGC 9 cut(s) 6, 9, 227, 245, 576, 636, 691, 850, 1378
Bme18I GGWCC 1 cut(s) 683
BmeT110I CYCGRG 2 cut(s) 581, 965
BmgT120I GGNCC 1 cut(s) 683
BmiI GGNNCC 2 cut(s) 123, 300
BmsI GCATC 3 cut(s) 828, 1046, 1398
BoxI GACNNNNGTC 1 cut(s) 672
BpiI GAAGAC 1 cut(s) 312
BplI GAGNNNNNCTC 2 cut(s) 32, 64
BpmI CTGGAG 1 cut(s) 1356
Bpu14I TTCGAA 2 cut(s) 1242, 1311
BpuEI CTTGAG 1 cut(s) 245
BsaJI CCNNGG 3 cut(s) 72, 897, 1471
BsaXI ACNNNNNCTCC 8 cut(s) 1145, 1175, 1187, 1214, 1217, 1244, 1256, 1286
Bsc4I CCNNNNNNNGG 1 cut(s) 664
Bse1I ACTGG 1 cut(s) 1451
Bse3DI GCAATG 2 cut(s) 97, 457
BseDI CCNNGG 3 cut(s) 72, 897, 1471
BseLI CCNNNNNNNGG 1 cut(s) 664
BseMI GCAATG 2 cut(s) 97, 457
BseMII CTCAG 2 cut(s) 226, 315
BseNI ACTGG 1 cut(s) 1451
BseRI GAGGAG 4 cut(s) 266, 431, 1208, 1235
BseXI GCAGC 5 cut(s) 16, 230, 701, 860, 1363
BseYI CCCAGC 1 cut(s) 1335
BsgI GTGCAG 1 cut(s) 1079
Bsh1236I CGCG 3 cut(s) 340, 381, 531
BshFI GGCC 2 cut(s) 615, 1462
BshNI GGYRCC 1 cut(s) 298
BsiHKAI GWGCWC 5 cut(s) 34, 45, 165, 330, 1498
BsiHKCI CYCGRG 2 cut(s) 581, 965
BslFI GGGAC 2 cut(s) 660, 1490
BslI CCNNNNNNNGG 1 cut(s) 664
BsmAI GTCTC 2 cut(s) 363, 1374
BsmFI GGGAC 2 cut(s) 660, 1490
BsmI GAATGC 1 cut(s) 624
BsnI GGCC 2 cut(s) 615, 1462
BsoBI CYCGRG 2 cut(s) 581, 965
Bsp119I TTCGAA 2 cut(s) 1242, 1311
Bsp1286I GDGCHC 5 cut(s) 34, 45, 165, 330, 1498
Bsp143I GATC 5 cut(s) 341, 425, 499, 934, 1126
Bsp19I CCATGG 2 cut(s) 897, 1471
Bsp68I TCGCGA 1 cut(s) 531
BspACI CCGC 9 cut(s) 7, 226, 250, 283, 379, 396, 575, 635, 763
BspANI GGCC 2 cut(s) 615, 1462
BspCNI CTCAG 2 cut(s) 225, 316
BspFNI CGCG 3 cut(s) 340, 381, 531
BspLI GGNNCC 2 cut(s) 123, 300
BspMI ACCTGC 1 cut(s) 648
BspPI GGATC 2 cut(s) 336, 1134
BspQI GCTCTTC 1 cut(s) 564
BspT104I TTCGAA 2 cut(s) 1242, 1311
BspT107I GGYRCC 1 cut(s) 298
BsrDI GCAATG 2 cut(s) 97, 457
BsrI ACTGG 1 cut(s) 1451
BssECI CCNNGG 3 cut(s) 72, 897, 1471
BssMI GATC 5 cut(s) 341, 425, 499, 934, 1126
BssT1I CCWWGG 2 cut(s) 897, 1471
Bst4CI ACNGT 5 cut(s) 76, 350, 781, 791, 812
Bst6I CTCTTC 2 cut(s) 537, 564
BstBI TTCGAA 2 cut(s) 1242, 1311
BstC8I GCNNGC 5 cut(s) 248, 436, 479, 483, 1346
BstDEI CTNAG 3 cut(s) 35, 212, 324
BstDSI CCRYGG 3 cut(s) 72, 897, 1471
BstFNI CGCG 3 cut(s) 340, 381, 531
BstHHI GCGC 1 cut(s) 259
BstKTI GATC 5 cut(s) 344, 428, 502, 937, 1129
BstMAI GTCTC 2 cut(s) 363, 1374
BstMBI GATC 5 cut(s) 341, 425, 499, 934, 1126
BstPAI GACNNNNGTC 1 cut(s) 672
BstUI CGCG 3 cut(s) 340, 381, 531
BstV1I GCAGC 5 cut(s) 16, 230, 701, 860, 1363
BstV2I GAAGAC 1 cut(s) 312
BsuI GTATCC 1 cut(s) 933
BsuRI GGCC 2 cut(s) 615, 1462
BtgI CCRYGG 3 cut(s) 72, 897, 1471
BtgZI GCGATG 1 cut(s) 396
BtsIMutI CAGTG 3 cut(s) 34, 374, 1047
BtuMI TCGCGA 1 cut(s) 531
BveI ACCTGC 1 cut(s) 648
Cac8I GCNNGC 5 cut(s) 248, 436, 479, 483, 1346
CfoI GCGC 1 cut(s) 259
Cfr13I GGNCC 1 cut(s) 683
CseI GACGC 1 cut(s) 346
Csp6I GTAC 4 cut(s) 151, 547, 916, 1143
CviAII CATG 6 cut(s) 664, 745, 898, 959, 1401, 1472
CviQI GTAC 4 cut(s) 151, 547, 916, 1143
DdeI CTNAG 3 cut(s) 35, 212, 324
DpnI GATC 5 cut(s) 343, 427, 501, 936, 1128
DpnII GATC 5 cut(s) 341, 425, 499, 934, 1126
Eam1104I CTCTTC 2 cut(s) 537, 564
EarI CTCTTC 2 cut(s) 537, 564
EciI GGCGGA 1 cut(s) 752
Ecl136II GAGCTC 2 cut(s) 163, 328
Eco130I CCWWGG 2 cut(s) 897, 1471
Eco24I GRGCYC 2 cut(s) 165, 330
Eco32I GATATC 1 cut(s) 1321
Eco47I GGWCC 1 cut(s) 683
Eco53kI GAGCTC 2 cut(s) 163, 328
Eco57I CTGAAG 1 cut(s) 324
Eco88I CYCGRG 2 cut(s) 581, 965
EcoICRI GAGCTC 2 cut(s) 163, 328
EcoRI GAATTC 1 cut(s) 875
EcoRV GATATC 1 cut(s) 1321
EcoT14I CCWWGG 2 cut(s) 897, 1471
EcoT38I GRGCYC 2 cut(s) 165, 330
ErhI CCWWGG 2 cut(s) 897, 1471
FaeI CATG 6 cut(s) 667, 748, 901, 962, 1404, 1475
FaqI GGGAC 2 cut(s) 660, 1490
FatI CATG 6 cut(s) 663, 744, 897, 958, 1400, 1471
FauI CCCGC 1 cut(s) 243
FbaI TGATCA 1 cut(s) 934
Fnu4HI GCNGC 9 cut(s) 5, 8, 226, 244, 575, 635, 690, 849, 1377
FriOI GRGCYC 2 cut(s) 165, 330
Fsp4HI GCNGC 9 cut(s) 5, 8, 226, 244, 575, 635, 690, 849, 1377
GlaI GCGC 1 cut(s) 258
GluI GCNGC 9 cut(s) 5, 8, 226, 244, 575, 635, 690, 849, 1377
GsaI CCCAGC 1 cut(s) 1339
GsuI CTGGAG 1 cut(s) 1356
HaeIII GGCC 2 cut(s) 615, 1462
HgaI GACGC 1 cut(s) 346
HhaI GCGC 1 cut(s) 259
Hin1II CATG 6 cut(s) 667, 748, 901, 962, 1404, 1475
Hin6I GCGC 1 cut(s) 257
HinP1I GCGC 1 cut(s) 257
HincII GTYRAC 1 cut(s) 996
HindII GTYRAC 1 cut(s) 996
HindIII AAGCTT 2 cut(s) 739, 1353
HinfI GANTC 6 cut(s) 238, 386, 903, 1001, 1165, 1234
HphI GGTGA 1 cut(s) 1112
Hpy166II GTNNAC 1 cut(s) 996
Hpy188I TCNGA 2 cut(s) 325, 874
Hpy8I GTNNAC 1 cut(s) 996
Hpy99I CGWCG 2 cut(s) 449, 1193
HpyAV CCTTC 2 cut(s) 551, 709
HpyCH4III ACNGT 5 cut(s) 76, 350, 781, 791, 812
HpyCH4IV ACGT 2 cut(s) 153, 1485
HpyCH4V TGCA 6 cut(s) 4, 90, 643, 819, 1060, 1411
HpyF3I CTNAG 3 cut(s) 35, 212, 324
HpySE526I ACGT 2 cut(s) 153, 1485
Hsp92II CATG 6 cut(s) 667, 748, 901, 962, 1404, 1475
HspAI GCGC 1 cut(s) 257
Ksp22I TGATCA 1 cut(s) 934
Kzo9I GATC 5 cut(s) 341, 425, 499, 934, 1126
LguI GCTCTTC 1 cut(s) 564
LmnI GCTCC 4 cut(s) 53, 127, 160, 333
Lsp1109I GCAGC 5 cut(s) 16, 230, 701, 860, 1363
LweI GCATC 3 cut(s) 828, 1046, 1398
MaeII ACGT 2 cut(s) 153, 1485
MaeIII GTNAC 6 cut(s) 371, 775, 795, 1100, 1116, 1184
MalI GATC 5 cut(s) 343, 427, 501, 936, 1128
MboI GATC 5 cut(s) 341, 425, 499, 934, 1126
MhlI GDGCHC 5 cut(s) 34, 45, 165, 330, 1498
MluCI AATT 8 cut(s) 270, 875, 954, 971, 1206, 1275, 1465, 1500
MlyI GAGTC 2 cut(s) 232, 1010
MmeI TCCRAC 5 cut(s) 58, 369, 450, 733, 1194
MroXI GAANNNNTTC 2 cut(s) 173, 453
MseI TTAA 3 cut(s) 1205, 1274, 1362
MspA1I CMGCKG 2 cut(s) 7, 1376
Mva1269I GAATGC 1 cut(s) 624
MvnI CGCG 3 cut(s) 340, 381, 531
NcoI CCATGG 2 cut(s) 897, 1471
NdeII GATC 5 cut(s) 341, 425, 499, 934, 1126
NlaIII CATG 6 cut(s) 667, 748, 901, 962, 1404, 1475
NlaIV GGNNCC 2 cut(s) 123, 300
NmeAIII GCCGAG 1 cut(s) 803
NmuCI GTSAC 4 cut(s) 371, 775, 1100, 1116
NruI TCGCGA 1 cut(s) 531
NspV TTCGAA 2 cut(s) 1242, 1311
PaeR7I CTCGAG 2 cut(s) 581, 965
PaqCI CACCTGC 1 cut(s) 648
PciSI GCTCTTC 1 cut(s) 564
PcsI WCGNNNNNNNCGW 1 cut(s) 915
PctI GAATGC 1 cut(s) 624
PdmI GAANNNNTTC 2 cut(s) 173, 453
PfeI GAWTC 4 cut(s) 386, 903, 1165, 1234
PflFI GACNNNGTC 2 cut(s) 659, 1000
PkrI GCNGC 9 cut(s) 6, 9, 227, 245, 576, 636, 691, 850, 1378
PleI GAGTC 2 cut(s) 232, 1009
PpsI GAGTC 2 cut(s) 232, 1009
PshAI GACNNNNGTC 1 cut(s) 672
PsiI TTATAA 1 cut(s) 110
Psp124BI GAGCTC 2 cut(s) 165, 330
Psp1406I AACGTT 1 cut(s) 1485
PspFI CCCAGC 1 cut(s) 1335
PspN4I GGNNCC 2 cut(s) 123, 300
PspPI GGNCC 1 cut(s) 683
PsyI GACNNNGTC 2 cut(s) 659, 1000
PvuII CAGCTG 1 cut(s) 1376
RruI TCGCGA 1 cut(s) 531
RsaI GTAC 4 cut(s) 152, 548, 917, 1144
RsaNI GTAC 4 cut(s) 151, 547, 916, 1143
SacI GAGCTC 2 cut(s) 165, 330
SapI GCTCTTC 1 cut(s) 564
SaqAI TTAA 3 cut(s) 1205, 1274, 1362
SatI GCNGC 9 cut(s) 5, 8, 226, 244, 575, 635, 690, 849, 1377
Sau3AI GATC 5 cut(s) 341, 425, 499, 934, 1126
Sau96I GGNCC 1 cut(s) 683
SchI GAGTC 2 cut(s) 232, 1010
SduI GDGCHC 5 cut(s) 34, 45, 165, 330, 1498
SfaNI GCATC 3 cut(s) 828, 1046, 1398
Sfr274I CTCGAG 2 cut(s) 581, 965
SfuI TTCGAA 2 cut(s) 1242, 1311
SinI GGWCC 1 cut(s) 683
SlaI CTCGAG 2 cut(s) 581, 965
SmlI CTYRAG 3 cut(s) 260, 581, 965
SmoI CTYRAG 3 cut(s) 260, 581, 965
Sse9I AATT 8 cut(s) 270, 875, 954, 971, 1206, 1275, 1465, 1500
SsiI CCGC 9 cut(s) 7, 226, 250, 283, 379, 396, 575, 635, 763
SstI GAGCTC 2 cut(s) 165, 330
StyI CCWWGG 2 cut(s) 897, 1471
TaaI ACNGT 5 cut(s) 76, 350, 781, 791, 812
TaiI ACGT 2 cut(s) 156, 1488
TasI AATT 8 cut(s) 270, 875, 954, 971, 1206, 1275, 1465, 1500
TatI WGTACW 2 cut(s) 546, 1142
TauI GCSGC 4 cut(s) 10, 228, 577, 637
TfiI GAWTC 4 cut(s) 386, 903, 1165, 1234
Tru1I TTAA 3 cut(s) 1205, 1274, 1362
Tru9I TTAA 3 cut(s) 1205, 1274, 1362
TscAI CASTG 3 cut(s) 34, 374, 1047
TseFI GTSAC 4 cut(s) 371, 775, 1100, 1116
TseI GCWGC 5 cut(s) 4, 243, 689, 848, 1376
Tsp45I GTSAC 4 cut(s) 371, 775, 1100, 1116
TspDTI ATGAA 5 cut(s) 446, 733, 734, 947, 1389
TspGWI ACGGA 6 cut(s) 40, 281, 308, 620, 924, 1013
TspRI CASTG 3 cut(s) 34, 374, 1047
Tth111I GACNNNGTC 2 cut(s) 659, 1000
VpaK11BI GGWCC 1 cut(s) 683
XapI RAATTY 4 cut(s) 875, 954, 971, 1500
XhoI CTCGAG 2 cut(s) 581, 965
XmnI GAANNNNTTC 2 cut(s) 173, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.