FvH4_4g33480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014618
Physical Location & Seq
Forward (+)
31701556 .. 31702495
940 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33480.t1

Sequence Viewer

Length: 558 bp
ATGTTGAAGCATTTCCAATCGGGAAGTTGGACTATGCTGGTCTATTTCCCACCCACATTACCATGTTATTCCCATCCTAAGGTCACCTCCTTTGAAACTGAAAGCAGATGTTCCGGTACATGCTCCGCTTTTAAAGCTTTCAGCAATGTCTTCAAACCAGGGGTCTACTCGAGTACTACTCGTTGCGCTGCTAGACGAAGAGTGAGATACGATGTCGACGAAGATGATGGGGATGAAGATGAAGAGGAAGAGCATAACAAGGAGATAGCACTGTTGGAGCTGTATAGTCAGAGTGTGAGAGGAGAAGCACTTATTGTTCATGCAATGGTGGATGACCAACCAGTGGAAGTGATTATCTTTAAGGGGTTTTCTTCATGCTTGAGTTACAGGACTTCACCAGACCCATCAAAAAGCATTCTTCCGGCAAGAGCAGTGATAGAGAGTATAGACAGGGTGAAGGGACCTTTTGACCCTTCAAATATAGAGTACTTGCAGAAAAACCTAAGCTGGGAAGTGTTCAAGAGCAATCTACCACCCAACTTACAGATGTATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

21.03

Weight (kDa)

5.26

Isoelectric Point (pI)

56.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7734 PF24869 90 - 177 4e-36 Domain of unknown function (DUF7734)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014618)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01755 AT2G01755
fragaria_vesca FvH4_4g33480
malus_domestica MD13G1019900.v1.1
prunus_persica Prupe.1G334100_v2.0.a1
pyrus_communis pycom13g01680
rosa_chinensis RchiOBHm_Chr4g0442791
rosa_laevigata RLG00000005984
rosa_multiflora Rmu_ssc0000204.1_g000020
rosa_roxburghii Rroxscaffold_5G00383570
rosa_rugosa Rorug04G0343100 Rorug04G0343200
rosa_samantha Rh4AG395700 Rh4BG408400 Rh4CG424200 Rh4DG402600
rosa_wichuraiana Rw4G034200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 343
AccI GTMKAC 2 cut(s) 165, 216
AciI CCGC 1 cut(s) 126
AfaI GTAC 3 cut(s) 118, 175, 488
AfiI CCNNNNNNNGG 3 cut(s) 79, 343, 508
AgsI TTSAA 5 cut(s) 7, 95, 154, 477, 520
AjnI CCWGG 1 cut(s) 157
AluBI AGCT 3 cut(s) 137, 280, 507
AluI AGCT 3 cut(s) 137, 280, 507
Ama87I CYCGRG 1 cut(s) 169
ApeKI GCWGC 1 cut(s) 188
ArsI GACNNNNNNTTYG 2 cut(s) 147, 179
Asp700I GAANNNNTTC 1 cut(s) 11
AspLEI GCGC 1 cut(s) 188
AspS9I GGNCC 1 cut(s) 461
AsuHPI GGTGA 3 cut(s) 76, 387, 466
AvaI CYCGRG 1 cut(s) 169
AvaII GGWCC 1 cut(s) 461
AxyI CCTNAGG 1 cut(s) 78
BbsI GAAGAC 1 cut(s) 142
BbvI GCAGC 1 cut(s) 175
BccI CCATC 3 cut(s) 81, 221, 412
BciT130I CCWGG 1 cut(s) 159
BfaI CTAG 1 cut(s) 192
BisI GCNGC 1 cut(s) 189
BlsI GCNGC 1 cut(s) 190
BmcAI AGTACT 2 cut(s) 175, 488
Bme1390I CCNGG 1 cut(s) 159
Bme18I GGWCC 1 cut(s) 461
BmeT110I CYCGRG 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 461
BmiI GGNNCC 1 cut(s) 462
BmrFI CCNGG 1 cut(s) 159
BpiI GAAGAC 1 cut(s) 142
BplI GAGNNNNNCTC 2 cut(s) 163, 195
Bpu10I CCTNAGC 1 cut(s) 503
BpuEI CTTGAG 1 cut(s) 400
BsaJI CCNNGG 1 cut(s) 158
BsaWI WCCGGW 1 cut(s) 113
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 343, 508
Bse1I ACTGG 1 cut(s) 341
Bse21I CCTNAGG 1 cut(s) 78
Bse3DI GCAATG 2 cut(s) 151, 330
BseBI CCWGG 1 cut(s) 159
BseDI CCNNGG 1 cut(s) 158
BseGI GGATG 3 cut(s) 73, 238, 337
BseLI CCNNNNNNNGG 3 cut(s) 79, 343, 508
BseMI GCAATG 2 cut(s) 151, 330
BseNI ACTGG 1 cut(s) 341
BseRI GAGGAG 1 cut(s) 315
BseXI GCAGC 1 cut(s) 175
BseYI CCCAGC 1 cut(s) 507
BsiHKCI CYCGRG 1 cut(s) 169
BsiSI CCGG 2 cut(s) 114, 422
BslFI GGGAC 1 cut(s) 474
BslI CCNNNNNNNGG 3 cut(s) 79, 343, 508
BsmFI GGGAC 1 cut(s) 474
BsmI GAATGC 1 cut(s) 414
BsoBI CYCGRG 1 cut(s) 169
BspACI CCGC 1 cut(s) 126
BspLI GGNNCC 1 cut(s) 462
BspQI GCTCTTC 1 cut(s) 243
BsrDI GCAATG 2 cut(s) 151, 330
BsrI ACTGG 1 cut(s) 341
BssECI CCNNGG 1 cut(s) 158
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 1 cut(s) 273
Bst6I CTCTTC 3 cut(s) 193, 237, 243
BstDEI CTNAG 2 cut(s) 78, 503
BstEII GGTNACC 1 cut(s) 82
BstF5I GGATG 3 cut(s) 73, 238, 337
BstHHI GCGC 1 cut(s) 188
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNI CCWGG 1 cut(s) 159
BstNSI RCATGY 1 cut(s) 123
BstPI GGTNACC 1 cut(s) 82
BstSCI CCNGG 1 cut(s) 157
BstV1I GCAGC 1 cut(s) 175
BstV2I GAAGAC 1 cut(s) 142
Bsu36I CCTNAGG 1 cut(s) 78
BtsCI GGATG 3 cut(s) 73, 238, 337
BtsI GCAGTG 1 cut(s) 438
BtsIMutI CAGTG 3 cut(s) 269, 348, 438
CfoI GCGC 1 cut(s) 188
Cfr13I GGNCC 1 cut(s) 461
Csp6I GTAC 3 cut(s) 117, 174, 487
CviAII CATG 4 cut(s) 63, 120, 320, 375
CviJI RGCY 3 cut(s) 137, 280, 507
CviKI_1 RGCY 3 cut(s) 137, 280, 507
CviQI GTAC 3 cut(s) 117, 174, 487
DdeI CTNAG 2 cut(s) 78, 503
DraI TTTAAA 1 cut(s) 133
Eam1104I CTCTTC 3 cut(s) 193, 237, 243
EarI CTCTTC 3 cut(s) 193, 237, 243
Eco47I GGWCC 1 cut(s) 461
Eco81I CCTNAGG 1 cut(s) 78
Eco88I CYCGRG 1 cut(s) 169
Eco91I GGTNACC 1 cut(s) 82
EcoO109I RGGNCCY 1 cut(s) 461
EcoO65I GGTNACC 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 157
FaeI CATG 4 cut(s) 66, 123, 323, 378
FaqI GGGAC 1 cut(s) 474
FatI CATG 4 cut(s) 62, 119, 319, 374
FblI GTMKAC 2 cut(s) 165, 216
Fnu4HI GCNGC 1 cut(s) 189
FokI GGATG 3 cut(s) 60, 245, 344
Fsp4HI GCNGC 1 cut(s) 189
FspBI CTAG 1 cut(s) 192
GlaI GCGC 1 cut(s) 187
GluI GCNGC 1 cut(s) 189
GsaI CCCAGC 1 cut(s) 511
HapII CCGG 2 cut(s) 114, 422
HhaI GCGC 1 cut(s) 188
Hin1II CATG 4 cut(s) 66, 123, 323, 378
Hin6I GCGC 1 cut(s) 186
HinP1I GCGC 1 cut(s) 186
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HindIII AAGCTT 1 cut(s) 135
HpaII CCGG 2 cut(s) 114, 422
HphI GGTGA 3 cut(s) 76, 387, 466
Hpy166II GTNNAC 2 cut(s) 166, 217
Hpy188I TCNGA 1 cut(s) 291
Hpy188III TCNNGA 2 cut(s) 21, 520
Hpy8I GTNNAC 2 cut(s) 166, 217
Hpy99I CGWCG 1 cut(s) 221
HpyAV CCTTC 2 cut(s) 451, 483
HpyCH4III ACNGT 1 cut(s) 273
HpyCH4V TGCA 2 cut(s) 323, 493
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 2 cut(s) 78, 503
Hsp92II CATG 4 cut(s) 66, 123, 323, 378
HspAI GCGC 1 cut(s) 186
LguI GCTCTTC 1 cut(s) 243
LmnI GCTCC 2 cut(s) 128, 277
Lsp1109I GCAGC 1 cut(s) 175
MaeI CTAG 1 cut(s) 192
MaeIII GTNAC 2 cut(s) 82, 383
MboII GAAGA 8 cut(s) 142, 210, 233, 248, 254, 260, 363, 410
MmeI TCCRAC 2 cut(s) 8, 255
MnlI CCTC 3 cut(s) 97, 238, 293
MroXI GAANNNNTTC 1 cut(s) 11
MseI TTAA 2 cut(s) 132, 360
MslI CAYNNNNRTG 1 cut(s) 61
MspI CCGG 2 cut(s) 114, 422
MspR9I CCNGG 1 cut(s) 159
Mva1269I GAATGC 1 cut(s) 414
MvaI CCWGG 1 cut(s) 159
MwoI GCNNNNNNNGC 1 cut(s) 134
NlaIII CATG 4 cut(s) 66, 123, 323, 378
NlaIV GGNNCC 1 cut(s) 462
NmuCI GTSAC 1 cut(s) 82
NspI RCATGY 1 cut(s) 123
PaeR7I CTCGAG 1 cut(s) 169
PciSI GCTCTTC 1 cut(s) 243
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PctI GAATGC 1 cut(s) 414
PdmI GAANNNNTTC 1 cut(s) 11
PflMI CCANNNNNTGG 1 cut(s) 343
PkrI GCNGC 1 cut(s) 190
PpuMI RGGWCCY 1 cut(s) 461
Psp5II RGGWCCY 1 cut(s) 461
Psp6I CCWGG 1 cut(s) 157
PspEI GGTNACC 1 cut(s) 82
PspFI CCCAGC 1 cut(s) 507
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 1 cut(s) 462
PspPI GGNCC 1 cut(s) 461
PspPPI RGGWCCY 1 cut(s) 461
PspXI VCTCGAGB 1 cut(s) 169
RsaI GTAC 3 cut(s) 118, 175, 488
RsaNI GTAC 3 cut(s) 117, 174, 487
RseI CAYNNNNRTG 1 cut(s) 61
SalI GTCGAC 1 cut(s) 215
SapI GCTCTTC 1 cut(s) 243
SaqAI TTAA 2 cut(s) 132, 360
SatI GCNGC 1 cut(s) 189
Sau96I GGNCC 1 cut(s) 461
ScaI AGTACT 2 cut(s) 175, 488
ScrFI CCNGG 1 cut(s) 159
SetI ASST 7 cut(s) 84, 89, 139, 282, 466, 504, 509
Sfr274I CTCGAG 1 cut(s) 169
SinI GGWCC 1 cut(s) 461
SlaI CTCGAG 1 cut(s) 169
SmiMI CAYNNNNRTG 1 cut(s) 61
SmlI CTYRAG 2 cut(s) 169, 379
SmoI CTYRAG 2 cut(s) 169, 379
SsiI CCGC 1 cut(s) 126
SspMI CTAG 1 cut(s) 192
StyD4I CCNGG 1 cut(s) 157
TaaI ACNGT 1 cut(s) 273
TaqI TCGA 2 cut(s) 170, 216
TatI WGTACW 2 cut(s) 173, 486
Tru1I TTAA 2 cut(s) 132, 360
Tru9I TTAA 2 cut(s) 132, 360
TscAI CASTG 3 cut(s) 276, 348, 438
TseFI GTSAC 1 cut(s) 82
TseI GCWGC 1 cut(s) 188
Tsp45I GTSAC 1 cut(s) 82
TspDTI ATGAA 4 cut(s) 249, 255, 308, 363
TspRI CASTG 3 cut(s) 276, 348, 438
Van91I CCANNNNNTGG 1 cut(s) 343
VpaK11BI GGWCC 1 cut(s) 461
XceI RCATGY 1 cut(s) 123
XhoI CTCGAG 1 cut(s) 169
XmiI GTMKAC 2 cut(s) 165, 216
XmnI GAANNNNTTC 1 cut(s) 11
XspI CTAG 1 cut(s) 192
ZrmI AGTACT 2 cut(s) 175, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.