MD13G1019900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014618
Physical Location & Seq
Forward (+)
1253875 .. 1254938
1064 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1019900.v1.1.491

Sequence Viewer

Length: 567 bp
ATGTTGAAGCATTTCCAATTGGGAAGATGGACTGTGCTGGTCTCCTCCCCACCCAAATCACCGTTTTCTCCAAATGCAACCACCATCACCTCCTCCTCCTCTGAATCTGCAGCTACCAGATATTTCAGTACATGCCGTGCCTCAGCCTTCAACGTGAATGCCTTTAGACCAAGGGGCTTGAGCACTCGATGCGCTGCCAGGCGGAGAGTGCTCAGATACGACGATGACGAGGATGATGATGAGGACGTCGAGTACGGACACAATAAGGAGATAGCAGTGTTGGAATTATACAGTCAGAGTGTGAGAGGAGAAGCACTTATCGTGCATGCATTGGTGGATGACCAGGATGTAGAAGTGCTTATCTTCAAGGGATTCTCTTCGAGCTTGAGTTATGGGACGTCACCGGACCCATCGAAGAGCATTCTTCCAGCAAGGGCAGTGATCAAGGCTATAGACAGGGTGAAAGGACCTTTTGACCCTTCTAATATTGAATACTTGCAGAAAGGCCTCACCTGGGAAGCGTTTAAGAGCACTACTCTACCCCCCAACTTACAACTCCCAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

189

Amino Acids

20.87

Weight (kDa)

6.3

Isoelectric Point (pI)

57.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7734 PF24869 92 - 177 5e-36 Domain of unknown function (DUF7734)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014618)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01755 AT2G01755
fragaria_vesca FvH4_4g33480
malus_domestica MD13G1019900.v1.1
prunus_persica Prupe.1G334100_v2.0.a1
pyrus_communis pycom13g01680
rosa_chinensis RchiOBHm_Chr4g0442791
rosa_laevigata RLG00000005984
rosa_multiflora Rmu_ssc0000204.1_g000020
rosa_roxburghii Rroxscaffold_5G00383570
rosa_rugosa Rorug04G0343100 Rorug04G0343200
rosa_samantha Rh4AG395700 Rh4BG408400 Rh4CG424200 Rh4DG402600
rosa_wichuraiana Rw4G034200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 249, 401
AciI CCGC 1 cut(s) 202
AcyI GRCGYC 2 cut(s) 246, 398
AfaI GTAC 2 cut(s) 130, 254
AfiI CCNNNNNNNGG 1 cut(s) 514
AgsI TTSAA 4 cut(s) 7, 151, 367, 491
AjnI CCWGG 3 cut(s) 197, 342, 512
AluBI AGCT 2 cut(s) 113, 384
AluI AGCT 2 cut(s) 113, 384
Alw21I GWGCWC 3 cut(s) 185, 213, 533
Alw26I GTCTC 1 cut(s) 46
AoxI GGCC 1 cut(s) 505
ApeKI GCWGC 2 cut(s) 110, 194
Asp700I GAANNNNTTC 1 cut(s) 11
AspLEI GCGC 1 cut(s) 194
AspS9I GGNCC 2 cut(s) 406, 467
AsuHPI GGTGA 5 cut(s) 51, 79, 393, 472, 502
AvaII GGWCC 2 cut(s) 406, 467
Bbv12I GWGCWC 3 cut(s) 185, 213, 533
BbvCI CCTCAGC 1 cut(s) 142
BbvI GCAGC 2 cut(s) 122, 181
BccI CCATC 3 cut(s) 21, 92, 418
BceAI ACGGC 1 cut(s) 120
BciT130I CCWGG 3 cut(s) 199, 344, 514
BclI TGATCA 1 cut(s) 441
BcoDI GTCTC 1 cut(s) 46
BfmI CTRYAG 2 cut(s) 108, 450
BisI GCNGC 2 cut(s) 111, 195
BlsI GCNGC 2 cut(s) 112, 196
Bme1390I CCNGG 3 cut(s) 199, 344, 514
Bme18I GGWCC 2 cut(s) 406, 467
BmgT120I GGNCC 2 cut(s) 406, 467
BmiI GGNNCC 1 cut(s) 408
BmrFI CCNGG 3 cut(s) 199, 344, 514
BmsI GCATC 1 cut(s) 179
BplI GAGNNNNNCTC 2 cut(s) 520, 552
Bpu10I CCTNAGC 1 cut(s) 142
BpuEI CTTGAG 2 cut(s) 199, 406
BsaHI GRCGYC 2 cut(s) 246, 398
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 2 cut(s) 170, 513
BsaWI WCCGGW 1 cut(s) 403
Bsc4I CCNNNNNNNGG 1 cut(s) 514
BseBI CCWGG 3 cut(s) 199, 344, 514
BseDI CCNNGG 2 cut(s) 170, 513
BseGI GGATG 3 cut(s) 238, 343, 352
BseLI CCNNNNNNNGG 1 cut(s) 514
BseMII CTCAG 2 cut(s) 156, 226
BseRI GAGGAG 5 cut(s) 34, 82, 85, 88, 321
BseXI GCAGC 2 cut(s) 122, 181
BshFI GGCC 1 cut(s) 507
BsiHKAI GWGCWC 3 cut(s) 185, 213, 533
BsiSI CCGG 1 cut(s) 404
BslFI GGGAC 1 cut(s) 409
BslI CCNNNNNNNGG 1 cut(s) 514
BsmAI GTCTC 1 cut(s) 46
BsmFI GGGAC 1 cut(s) 409
BsmI GAATGC 2 cut(s) 163, 420
BsnI GGCC 1 cut(s) 507
Bso31I GGTCTC 1 cut(s) 46
Bsp1286I GDGCHC 3 cut(s) 185, 213, 533
Bsp143I GATC 1 cut(s) 441
BspACI CCGC 1 cut(s) 202
BspANI GGCC 1 cut(s) 507
BspCNI CTCAG 2 cut(s) 155, 225
BspLI GGNNCC 1 cut(s) 408
BspMAI CTGCAG 1 cut(s) 112
BspQI GCTCTTC 1 cut(s) 410
BspTNI GGTCTC 1 cut(s) 46
BssECI CCNNGG 2 cut(s) 170, 513
BssMI GATC 1 cut(s) 441
BssNI GRCGYC 2 cut(s) 246, 398
BssT1I CCWWGG 1 cut(s) 170
Bst2UI CCWGG 3 cut(s) 199, 344, 514
Bst4CI ACNGT 3 cut(s) 34, 63, 293
Bst6I CTCTTC 2 cut(s) 382, 410
BstACI GRCGYC 2 cut(s) 246, 398
BstAPI GCANNNNNTGC 1 cut(s) 189
BstC8I GCNNGC 1 cut(s) 327
BstDEI CTNAG 2 cut(s) 142, 212
BstF5I GGATG 3 cut(s) 238, 343, 352
BstHHI GCGC 1 cut(s) 194
BstKTI GATC 1 cut(s) 444
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 1 cut(s) 441
BstMWI GCNNNNNNNGC 2 cut(s) 189, 208
BstNI CCWGG 3 cut(s) 199, 344, 514
BstNSI RCATGY 2 cut(s) 135, 329
BstSCI CCNGG 3 cut(s) 197, 342, 512
BstSFI CTRYAG 2 cut(s) 108, 450
BstV1I GCAGC 2 cut(s) 122, 181
BsuRI GGCC 1 cut(s) 507
BtsCI GGATG 3 cut(s) 238, 343, 352
BtsI GCAGTG 2 cut(s) 282, 444
BtsIMutI CAGTG 2 cut(s) 282, 444
Cac8I GCNNGC 1 cut(s) 327
CfoI GCGC 1 cut(s) 194
Cfr13I GGNCC 2 cut(s) 406, 467
Csp6I GTAC 2 cut(s) 129, 253
CviAII CATG 2 cut(s) 132, 326
CviJI RGCY 6 cut(s) 113, 146, 177, 384, 449, 507
CviKI_1 RGCY 6 cut(s) 113, 146, 177, 384, 449, 507
CviQI GTAC 2 cut(s) 129, 253
DdeI CTNAG 2 cut(s) 142, 212
DpnI GATC 1 cut(s) 443
DpnII GATC 1 cut(s) 441
Eam1104I CTCTTC 2 cut(s) 382, 410
EarI CTCTTC 2 cut(s) 382, 410
EciI GGCGGA 1 cut(s) 217
Eco130I CCWWGG 1 cut(s) 170
Eco147I AGGCCT 1 cut(s) 507
Eco31I GGTCTC 1 cut(s) 46
Eco47I GGWCC 2 cut(s) 406, 467
EcoO109I RGGNCCY 1 cut(s) 467
EcoRII CCWGG 3 cut(s) 197, 342, 512
EcoT14I CCWWGG 1 cut(s) 170
EcoT22I ATGCAT 1 cut(s) 331
ErhI CCWWGG 1 cut(s) 170
FaeI CATG 2 cut(s) 135, 329
FaiI YATR 5 cut(s) 133, 289, 327, 393, 452
FaqI GGGAC 1 cut(s) 409
FatI CATG 2 cut(s) 131, 325
FbaI TGATCA 1 cut(s) 441
Fnu4HI GCNGC 2 cut(s) 111, 195
FokI GGATG 3 cut(s) 245, 350, 359
Fsp4HI GCNGC 2 cut(s) 111, 195
GlaI GCGC 1 cut(s) 193
GluI GCNGC 2 cut(s) 111, 195
HaeIII GGCC 1 cut(s) 507
HapII CCGG 1 cut(s) 404
HhaI GCGC 1 cut(s) 194
Hin1I GRCGYC 2 cut(s) 246, 398
Hin1II CATG 2 cut(s) 135, 329
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HinfI GANTC 2 cut(s) 104, 372
HpaII CCGG 1 cut(s) 404
HphI GGTGA 5 cut(s) 51, 79, 393, 472, 502
Hpy188I TCNGA 3 cut(s) 103, 215, 297
Hpy99I CGWCG 2 cut(s) 224, 251
HpyAV CCTTC 2 cut(s) 157, 489
HpyCH4III ACNGT 3 cut(s) 34, 63, 293
HpyCH4IV ACGT 3 cut(s) 153, 246, 398
HpyCH4V TGCA 5 cut(s) 77, 110, 325, 329, 499
HpyF10VI GCNNNNNNNGC 2 cut(s) 189, 208
HpyF3I CTNAG 2 cut(s) 142, 212
HpySE526I ACGT 3 cut(s) 153, 246, 398
Hsp92I GRCGYC 2 cut(s) 246, 398
Hsp92II CATG 2 cut(s) 135, 329
HspAI GCGC 1 cut(s) 192
Ksp22I TGATCA 1 cut(s) 441
Kzo9I GATC 1 cut(s) 441
LguI GCTCTTC 1 cut(s) 410
Lsp1109I GCAGC 2 cut(s) 122, 181
LweI GCATC 1 cut(s) 179
MaeII ACGT 3 cut(s) 153, 246, 398
MaeIII GTNAC 1 cut(s) 399
MalI GATC 1 cut(s) 443
MboI GATC 1 cut(s) 441
MboII GAAGA 5 cut(s) 36, 355, 369, 416, 427
MfeI CAATTG 1 cut(s) 17
MhlI GDGCHC 3 cut(s) 185, 213, 533
MluCI AATT 3 cut(s) 17, 284, 561
MmeI TCCRAC 1 cut(s) 261
Mph1103I ATGCAT 1 cut(s) 331
MroXI GAANNNNTTC 1 cut(s) 11
MseI TTAA 1 cut(s) 525
MspI CCGG 1 cut(s) 404
MspR9I CCNGG 3 cut(s) 199, 344, 514
MunI CAATTG 1 cut(s) 17
Mva1269I GAATGC 2 cut(s) 163, 420
MvaI CCWGG 3 cut(s) 199, 344, 514
MwoI GCNNNNNNNGC 2 cut(s) 189, 208
NdeII GATC 1 cut(s) 441
NlaIII CATG 2 cut(s) 135, 329
NlaIV GGNNCC 1 cut(s) 408
NmuCI GTSAC 1 cut(s) 399
NsiI ATGCAT 1 cut(s) 331
NspI RCATGY 2 cut(s) 135, 329
PaeI GCATGC 1 cut(s) 329
PceI AGGCCT 1 cut(s) 507
PciSI GCTCTTC 1 cut(s) 410
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PctI GAATGC 2 cut(s) 163, 420
PdmI GAANNNNTTC 1 cut(s) 11
PfeI GAWTC 2 cut(s) 104, 372
PkrI GCNGC 2 cut(s) 112, 196
PpuMI RGGWCCY 1 cut(s) 467
Psp5II RGGWCCY 1 cut(s) 467
Psp6I CCWGG 3 cut(s) 197, 342, 512
PspGI CCWGG 3 cut(s) 197, 342, 512
PspN4I GGNNCC 1 cut(s) 408
PspPI GGNCC 2 cut(s) 406, 467
PspPPI RGGWCCY 1 cut(s) 467
PstI CTGCAG 1 cut(s) 112
RsaI GTAC 2 cut(s) 130, 254
RsaNI GTAC 2 cut(s) 129, 253
SapI GCTCTTC 1 cut(s) 410
SaqAI TTAA 1 cut(s) 525
SatI GCNGC 2 cut(s) 111, 195
Sau3AI GATC 1 cut(s) 441
Sau96I GGNCC 2 cut(s) 406, 467
ScrFI CCNGG 3 cut(s) 199, 344, 514
SduI GDGCHC 3 cut(s) 185, 213, 533
SetI ASST 8 cut(s) 92, 115, 156, 249, 386, 401, 472, 515
SfaNI GCATC 1 cut(s) 179
SfcI CTRYAG 2 cut(s) 108, 450
SinI GGWCC 2 cut(s) 406, 467
SmlI CTYRAG 2 cut(s) 178, 385
SmoI CTYRAG 2 cut(s) 178, 385
SphI GCATGC 1 cut(s) 329
Sse9I AATT 3 cut(s) 17, 284, 561
SseBI AGGCCT 1 cut(s) 507
SsiI CCGC 1 cut(s) 202
SspI AATATT 1 cut(s) 487
StuI AGGCCT 1 cut(s) 507
StyD4I CCNGG 3 cut(s) 197, 342, 512
StyI CCWWGG 1 cut(s) 170
TaaI ACNGT 3 cut(s) 34, 63, 293
TaiI ACGT 3 cut(s) 156, 249, 401
TaqI TCGA 4 cut(s) 187, 249, 380, 413
TasI AATT 3 cut(s) 17, 284, 561
TatI WGTACW 1 cut(s) 128
TfiI GAWTC 2 cut(s) 104, 372
Tru1I TTAA 1 cut(s) 525
Tru9I TTAA 1 cut(s) 525
TscAI CASTG 2 cut(s) 282, 444
TseFI GTSAC 1 cut(s) 399
TseI GCWGC 2 cut(s) 110, 194
Tsp45I GTSAC 1 cut(s) 399
TspGWI ACGGA 1 cut(s) 270
TspRI CASTG 2 cut(s) 282, 444
VpaK11BI GGWCC 2 cut(s) 406, 467
XceI RCATGY 2 cut(s) 135, 329
XmnI GAANNNNTTC 1 cut(s) 11
ZraI GACGTC 2 cut(s) 247, 399
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.