FvH4_4g34391

ER-associated misfolded protein catabolic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0017190
Physical Location & Seq
Forward (+)
32212225 .. 32212686
462 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g34391.t1

Sequence Viewer

Length: 462 bp
ATGGTTTCACTGAAGCGCCGCCGTGCAGGGATGCGTGTCATTTTGCCCCAACCCCATATGATCTTCCATTTCACAAAGCCCCAAGATTTCGATCACCTCGGTGACGATGATGTCGTAGGCGACAGTTCCGTGGAATCCGATGCATGTTCTGAGACTAGTCCCGACCACAATTCTTGCAAGAAAGACAGTCATTCTCAGTCTCAACCAGAACAGAATGGTAAGAGTAGCAACAACAGCTCCGGTTCTGCGAAGATGTCGTATTTCGATTGTAACTTGTGTTTCAAATCGGAGAGGCAGCCGGTTGTGACGGCGTGCGGGCATTTGTTTTGCCATTCTTGCTTGTGCGACTGGCTCGAGTTTCGTCGGAAGTGTCCTGTCTGTGAGGCGGAGGTGGTTGACGGACGCATCTTTCCGATTTACCAATGCAGTGGACCGAGATCGAAGTATTATGGTACCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4 zf-C3HC4_3

Protein Analysis

154

Amino Acids

17.22

Weight (kDa)

7.01

Isoelectric Point (pI)

77.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 89 - 131 1.9e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 89 - 127 5.5e-08 Ring finger domain
zf-C3HC4_2 PF13923 90 - 127 9.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 90 - 128 2.1e-07 zinc-RING finger domain
zf-C3HC4 PF00097 90 - 127 2.3e-07 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 90 - 134 4.1e-06 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017190)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74990
fragaria_vesca FvH4_4g34391
malus_domestica MD02G1215900.v1.1
prunus_persica Prupe.8G036400_v2.0.a1
pyrus_communis pycom07g09040
rosa_chinensis RchiOBHm_Chr4g0443821
rosa_laevigata RLG00000005885
rosa_roxburghii Rroxscaffold_5G00384390
rosa_rugosa Rorug04G0347600
rosa_samantha Rh4AG407800 Rh4BG418800 Rh4CG432900 Rh4DG414000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
Acc65I GGTACC 1 cut(s) 452
AccB1I GGYRCC 1 cut(s) 452
AciI CCGC 3 cut(s) 19, 315, 386
AcuI CTGAAG 1 cut(s) 32
AfaI GTAC 1 cut(s) 454
AgsI TTSAA 1 cut(s) 283
AhlI ACTAGT 1 cut(s) 155
AleI CACNNNNGTG 1 cut(s) 99
AluBI AGCT 1 cut(s) 237
AluI AGCT 1 cut(s) 237
Alw26I GTCTC 2 cut(s) 146, 204
Ama87I CYCGRG 1 cut(s) 353
ApeKI GCWGC 1 cut(s) 295
Asp718I GGTACC 1 cut(s) 452
AspLEI GCGC 1 cut(s) 18
AspS9I GGNCC 1 cut(s) 431
AsuHPI GGTGA 2 cut(s) 86, 113
AvaI CYCGRG 1 cut(s) 353
AvaII GGWCC 1 cut(s) 431
BanI GGYRCC 1 cut(s) 452
BbvI GCAGC 1 cut(s) 307
BceAI ACGGC 2 cut(s) 6, 324
BcoDI GTCTC 2 cut(s) 146, 204
BcuI ACTAGT 1 cut(s) 155
BfaI CTAG 1 cut(s) 156
BfoI RGCGCY 1 cut(s) 19
BisI GCNGC 2 cut(s) 19, 296
BlsI GCNGC 2 cut(s) 20, 297
Bme18I GGWCC 1 cut(s) 431
BmeT110I CYCGRG 1 cut(s) 353
BmgT120I GGNCC 1 cut(s) 431
BmiI GGNNCC 1 cut(s) 454
BmsI GCATC 3 cut(s) 21, 130, 414
BsaBI GATNNNNATC 1 cut(s) 90
BsaJI CCNNGG 2 cut(s) 97, 129
BsaWI WCCGGW 1 cut(s) 239
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bse118I RCCGGY 1 cut(s) 298
Bse1I ACTGG 1 cut(s) 353
Bse8I GATNNNNATC 1 cut(s) 90
BseDI CCNNGG 2 cut(s) 97, 129
BseGI GGATG 1 cut(s) 36
BseJI GATNNNNATC 1 cut(s) 90
BseMII CTCAG 2 cut(s) 141, 209
BseNI ACTGG 1 cut(s) 353
BseXI GCAGC 1 cut(s) 307
BsgI GTGCAG 1 cut(s) 45
BshNI GGYRCC 1 cut(s) 452
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 2 cut(s) 240, 299
BslFI GGGAC 1 cut(s) 144
BsmAI GTCTC 2 cut(s) 146, 204
BsmFI GGGAC 1 cut(s) 144
BsoBI CYCGRG 1 cut(s) 353
Bsp143I GATC 3 cut(s) 60, 91, 437
BspACI CCGC 3 cut(s) 19, 315, 386
BspCNI CTCAG 2 cut(s) 142, 208
BspLI GGNNCC 1 cut(s) 454
BspT107I GGYRCC 1 cut(s) 452
BsrFI RCCGGY 1 cut(s) 298
BsrI ACTGG 1 cut(s) 353
BssAI RCCGGY 1 cut(s) 298
BssECI CCNNGG 2 cut(s) 97, 129
BssMI GATC 3 cut(s) 60, 91, 437
Bst4CI ACNGT 2 cut(s) 125, 188
BstC8I GCNNGC 2 cut(s) 313, 317
BstDEI CTNAG 2 cut(s) 150, 195
BstDSI CCRYGG 1 cut(s) 129
BstF5I GGATG 1 cut(s) 36
BstH2I RGCGCY 1 cut(s) 19
BstHHI GCGC 1 cut(s) 18
BstKTI GATC 3 cut(s) 63, 94, 440
BstMAI GTCTC 2 cut(s) 146, 204
BstMBI GATC 3 cut(s) 60, 91, 437
BstMWI GCNNNNNNNGC 2 cut(s) 234, 336
BstNSI RCATGY 1 cut(s) 147
BstV1I GCAGC 1 cut(s) 307
BstXI CCANNNNNNTGG 1 cut(s) 428
BtgI CCRYGG 1 cut(s) 129
BtsCI GGATG 1 cut(s) 36
BtsI GCAGTG 1 cut(s) 433
BtsIMutI CAGTG 2 cut(s) 8, 433
Cac8I GCNNGC 2 cut(s) 313, 317
CfoI GCGC 1 cut(s) 18
Cfr10I RCCGGY 1 cut(s) 298
Cfr13I GGNCC 1 cut(s) 431
CseI GACGC 1 cut(s) 411
Csp6I GTAC 1 cut(s) 453
CspCI CAANNNNNGTGG 2 cut(s) 155, 190
CviAII CATG 1 cut(s) 144
CviJI RGCY 4 cut(s) 79, 237, 298, 352
CviKI_1 RGCY 4 cut(s) 79, 237, 298, 352
CviQI GTAC 1 cut(s) 453
DdeI CTNAG 2 cut(s) 150, 195
DpnI GATC 3 cut(s) 62, 93, 439
DpnII GATC 3 cut(s) 60, 91, 437
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
EciI GGCGGA 1 cut(s) 401
Eco47I GGWCC 1 cut(s) 431
Eco57I CTGAAG 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 353
EcoT22I ATGCAT 1 cut(s) 145
FaeI CATG 1 cut(s) 147
FaiI YATR 4 cut(s) 57, 59, 145, 450
FaqI GGGAC 1 cut(s) 144
FatI CATG 1 cut(s) 143
FauI CCCGC 1 cut(s) 308
FauNDI CATATG 1 cut(s) 57
Fnu4HI GCNGC 2 cut(s) 19, 296
FokI GGATG 1 cut(s) 43
Fsp4HI GCNGC 2 cut(s) 19, 296
FspBI CTAG 1 cut(s) 156
GlaI GCGC 1 cut(s) 17
GluI GCNGC 2 cut(s) 19, 296
HaeII RGCGCY 1 cut(s) 19
HapII CCGG 2 cut(s) 240, 299
HgaI GACGC 1 cut(s) 411
HhaI GCGC 1 cut(s) 18
Hin1II CATG 1 cut(s) 147
Hin6I GCGC 1 cut(s) 16
HinP1I GCGC 1 cut(s) 16
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HinfI GANTC 1 cut(s) 134
HpaII CCGG 2 cut(s) 240, 299
HphI GGTGA 2 cut(s) 86, 113
Hpy166II GTNNAC 2 cut(s) 397, 431
Hpy188I TCNGA 5 cut(s) 139, 151, 289, 366, 414
Hpy188III TCNNGA 1 cut(s) 161
Hpy8I GTNNAC 2 cut(s) 397, 431
Hpy99I CGWCG 1 cut(s) 366
HpyCH4III ACNGT 2 cut(s) 125, 188
HpyCH4V TGCA 4 cut(s) 26, 143, 177, 426
HpyF10VI GCNNNNNNNGC 2 cut(s) 234, 336
HpyF3I CTNAG 2 cut(s) 150, 195
Hsp92II CATG 1 cut(s) 147
HspAI GCGC 1 cut(s) 16
KpnI GGTACC 1 cut(s) 456
Kzo9I GATC 3 cut(s) 60, 91, 437
LmnI GCTCC 1 cut(s) 242
LpnPI CCDG 6 cut(s) 12, 219, 253, 312, 334, 387
Lsp1109I GCAGC 1 cut(s) 307
LweI GCATC 3 cut(s) 21, 130, 414
MaeI CTAG 1 cut(s) 156
MaeIII GTNAC 3 cut(s) 101, 269, 304
MalI GATC 3 cut(s) 62, 93, 439
MboI GATC 3 cut(s) 60, 91, 437
MboII GAAGA 2 cut(s) 55, 262
MluCI AATT 1 cut(s) 169
MmeI TCCRAC 1 cut(s) 344
MnlI CCTC 4 cut(s) 107, 285, 376, 382
Mph1103I ATGCAT 1 cut(s) 145
MslI CAYNNNNRTG 1 cut(s) 99
MspI CCGG 2 cut(s) 240, 299
MwoI GCNNNNNNNGC 2 cut(s) 234, 336
NdeI CATATG 1 cut(s) 57
NdeII GATC 3 cut(s) 60, 91, 437
NlaIII CATG 1 cut(s) 147
NlaIV GGNNCC 1 cut(s) 454
NmuCI GTSAC 2 cut(s) 101, 304
NsiI ATGCAT 1 cut(s) 145
NspI RCATGY 1 cut(s) 147
OliI CACNNNNGTG 1 cut(s) 99
PaeR7I CTCGAG 1 cut(s) 353
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PfeI GAWTC 1 cut(s) 134
PkrI GCNGC 2 cut(s) 20, 297
PspN4I GGNNCC 1 cut(s) 454
PspPI GGNCC 1 cut(s) 431
PspXI VCTCGAGB 1 cut(s) 353
RsaI GTAC 1 cut(s) 454
RsaNI GTAC 1 cut(s) 453
RseI CAYNNNNRTG 1 cut(s) 99
SatI GCNGC 2 cut(s) 19, 296
Sau3AI GATC 3 cut(s) 60, 91, 437
Sau96I GGNCC 1 cut(s) 431
SetI ASST 3 cut(s) 99, 239, 393
SfaNI GCATC 3 cut(s) 21, 130, 414
Sfr274I CTCGAG 1 cut(s) 353
SinI GGWCC 1 cut(s) 431
SlaI CTCGAG 1 cut(s) 353
SmiMI CAYNNNNRTG 1 cut(s) 99
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
SpeI ACTAGT 1 cut(s) 155
Sse9I AATT 1 cut(s) 169
SsiI CCGC 3 cut(s) 19, 315, 386
SspMI CTAG 1 cut(s) 156
TaaI ACNGT 2 cut(s) 125, 188
TaqI TCGA 4 cut(s) 90, 264, 354, 440
TaqII GACCGA 1 cut(s) 448
TasI AATT 1 cut(s) 169
TauI GCSGC 1 cut(s) 21
TfiI GAWTC 1 cut(s) 134
TscAI CASTG 2 cut(s) 15, 433
TseFI GTSAC 2 cut(s) 101, 304
TseI GCWGC 1 cut(s) 295
Tsp45I GTSAC 2 cut(s) 101, 304
TspGWI ACGGA 2 cut(s) 118, 414
TspRI CASTG 2 cut(s) 15, 433
VpaK11BI GGWCC 1 cut(s) 431
XceI RCATGY 1 cut(s) 147
XhoI CTCGAG 1 cut(s) 353
XspI CTAG 1 cut(s) 156
Zsp2I ATGCAT 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.