RLG00000005885

ER-associated misfolded protein catabolic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0017190
Physical Location & Seq
Reverse (-)
2558662 .. 2560042
1381 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005885

Sequence Viewer

Length: 534 bp
ATGGGTTCCAACGAAGACAGTGCAAAACCACCGAAGCGCCGCCGCGCACGGATGAGTGTCATTTTGCCTCAACCCCATATGATTTTCAGGTTCCCAAAGCCTGAAGACTACAACCACCTCGATGACGACGTCGTAGACGATCATCACCCTCCTCTCTCCGAAGAATCCGATGCATGTTTGGAAACTAGTCCCGATCACAATCCTCGTCATGAAAATGGTACTACCAGCAGCTCCAGACCTGCAACGGCGTCGTATTTCGATTGTAACTTGTGCTTCAAATCGGAGAGGCAGCCGGTTGTGACTGCGTGCGGGCATTTGTATTGCTACTCTTGCTTGTGCGACTGCCTGCAGTTTGATCGGAAATGTCCTGTTTGTGAGGCCAAGGTGGTTGATGGACGCATCTTTCCTATTTATCAATGCAGTGGACCGAGGCCGAAGACACAGTTGGTGCAATTTTCTATCGCCATTCCTGGCTTGACTCATCCTCAGTTTGGAATATGTATACGCGATCCCAGTGACCGCACCGCGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4 zf-C3HC4_3

Protein Analysis

178

Amino Acids

19.89

Weight (kDa)

6.22

Isoelectric Point (pI)

61.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 87 - 129 2.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 88 - 126 2.3e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017190)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74990
fragaria_vesca FvH4_4g34391
malus_domestica MD02G1215900.v1.1
prunus_persica Prupe.8G036400_v2.0.a1
pyrus_communis pycom07g09040
rosa_chinensis RchiOBHm_Chr4g0443821
rosa_laevigata RLG00000005885
rosa_roxburghii Rroxscaffold_5G00384390
rosa_rugosa Rorug04G0347600
rosa_samantha Rh4AG407800 Rh4BG418800 Rh4CG432900 Rh4DG414000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 132
Acc36I ACCTGC 1 cut(s) 247
AccI GTMKAC 2 cut(s) 135, 502
AccII CGCG 3 cut(s) 45, 507, 527
AciI CCGC 6 cut(s) 40, 43, 309, 520, 525, 527
AclWI GGATC 1 cut(s) 503
AcuI CTGAAG 1 cut(s) 123
AcyI GRCGYC 2 cut(s) 129, 248
AfaI GTAC 1 cut(s) 220
AfiI CCNNNNNNNGG 2 cut(s) 491, 526
AgsI TTSAA 1 cut(s) 277
AhlI ACTAGT 1 cut(s) 185
AjnI CCWGG 1 cut(s) 469
AjuI GAANNNNNNNTTGG 2 cut(s) 428, 460
AluBI AGCT 1 cut(s) 231
AluI AGCT 1 cut(s) 231
AlwI GGATC 1 cut(s) 503
AoxI GGCC 2 cut(s) 378, 431
ApeKI GCWGC 2 cut(s) 228, 289
AspLEI GCGC 2 cut(s) 39, 47
AspS9I GGNCC 1 cut(s) 425
AsuHPI GGTGA 1 cut(s) 137
AvaII GGWCC 1 cut(s) 425
BbsI GAAGAC 3 cut(s) 21, 111, 443
BbvI GCAGC 2 cut(s) 240, 301
BccI CCATC 1 cut(s) 386
BceAI ACGGC 1 cut(s) 261
BcgI CGANNNNNNTGC 5 cut(s) 36, 231, 265, 338, 372
BciT130I CCWGG 1 cut(s) 471
BcuI ACTAGT 1 cut(s) 185
BfaI CTAG 1 cut(s) 186
BfmI CTRYAG 1 cut(s) 347
BfoI RGCGCY 1 cut(s) 40
BfuAI ACCTGC 1 cut(s) 247
BisI GCNGC 5 cut(s) 40, 43, 229, 290, 528
BlsI GCNGC 5 cut(s) 41, 44, 230, 291, 529
Bme1390I CCNGG 1 cut(s) 471
Bme18I GGWCC 1 cut(s) 425
BmgT120I GGNCC 1 cut(s) 425
BmiI GGNNCC 2 cut(s) 7, 92
BmrFI CCNGG 1 cut(s) 471
BmrI ACTGGG 1 cut(s) 507
BmsI GCATC 2 cut(s) 160, 408
BmuI ACTGGG 1 cut(s) 507
BpiI GAAGAC 3 cut(s) 21, 111, 443
BpmI CTGGAG 1 cut(s) 217
BsaBI GATNNNNATC 1 cut(s) 198
BsaHI GRCGYC 2 cut(s) 129, 248
BsaJI CCNNGG 3 cut(s) 381, 428, 525
Bsc4I CCNNNNNNNGG 2 cut(s) 491, 526
Bse118I RCCGGY 1 cut(s) 292
Bse1I ACTGG 1 cut(s) 513
Bse8I GATNNNNATC 1 cut(s) 198
BseBI CCWGG 1 cut(s) 471
BseDI CCNNGG 3 cut(s) 381, 428, 525
BseGI GGATG 2 cut(s) 57, 481
BseJI GATNNNNATC 1 cut(s) 198
BseLI CCNNNNNNNGG 2 cut(s) 491, 526
BseMII CTCAG 1 cut(s) 500
BseNI ACTGG 1 cut(s) 513
BseRI GAGGAG 1 cut(s) 141
BseXI GCAGC 2 cut(s) 240, 301
Bsh1236I CGCG 3 cut(s) 45, 507, 527
BshFI GGCC 2 cut(s) 380, 433
BsiSI CCGG 1 cut(s) 293
BslFI GGGAC 1 cut(s) 174
BslI CCNNNNNNNGG 2 cut(s) 491, 526
BsmFI GGGAC 1 cut(s) 174
BsnI GGCC 2 cut(s) 380, 433
Bsp143I GATC 4 cut(s) 139, 193, 355, 508
BspACI CCGC 6 cut(s) 40, 43, 309, 520, 525, 527
BspANI GGCC 2 cut(s) 380, 433
BspCNI CTCAG 1 cut(s) 499
BspFNI CGCG 3 cut(s) 45, 507, 527
BspHI TCATGA 1 cut(s) 208
BspLI GGNNCC 2 cut(s) 7, 92
BspMAI CTGCAG 1 cut(s) 351
BspMI ACCTGC 1 cut(s) 247
BspPI GGATC 1 cut(s) 503
BsrFI RCCGGY 1 cut(s) 292
BsrI ACTGG 1 cut(s) 513
BssAI RCCGGY 1 cut(s) 292
BssECI CCNNGG 3 cut(s) 381, 428, 525
BssMI GATC 4 cut(s) 139, 193, 355, 508
BssNAI GTATAC 1 cut(s) 503
BssNI GRCGYC 2 cut(s) 129, 248
BssT1I CCWWGG 1 cut(s) 381
Bst1107I GTATAC 1 cut(s) 503
Bst2UI CCWGG 1 cut(s) 471
Bst4CI ACNGT 2 cut(s) 20, 444
BstACI GRCGYC 2 cut(s) 129, 248
BstC8I GCNNGC 3 cut(s) 307, 311, 347
BstDEI CTNAG 1 cut(s) 486
BstDSI CCRYGG 1 cut(s) 525
BstF5I GGATG 2 cut(s) 57, 481
BstFNI CGCG 3 cut(s) 45, 507, 527
BstH2I RGCGCY 1 cut(s) 40
BstHHI GCGC 2 cut(s) 39, 47
BstKTI GATC 4 cut(s) 142, 196, 358, 511
BstMBI GATC 4 cut(s) 139, 193, 355, 508
BstMWI GCNNNNNNNGC 1 cut(s) 330
BstNI CCWGG 1 cut(s) 471
BstNSI RCATGY 1 cut(s) 177
BstSCI CCNGG 1 cut(s) 469
BstSFI CTRYAG 1 cut(s) 347
BstUI CGCG 3 cut(s) 45, 507, 527
BstV1I GCAGC 2 cut(s) 240, 301
BstV2I GAAGAC 3 cut(s) 21, 111, 443
BstZ17I GTATAC 1 cut(s) 503
BsuRI GGCC 2 cut(s) 380, 433
BtgI CCRYGG 1 cut(s) 525
BtsCI GGATG 2 cut(s) 57, 481
BtsI GCAGTG 1 cut(s) 427
BtsIMutI CAGTG 3 cut(s) 25, 427, 520
BveI ACCTGC 1 cut(s) 247
Cac8I GCNNGC 3 cut(s) 307, 311, 347
CciI TCATGA 1 cut(s) 208
CfoI GCGC 2 cut(s) 39, 47
Cfr10I RCCGGY 1 cut(s) 292
Cfr13I GGNCC 1 cut(s) 425
Cfr42I CCGCGG 1 cut(s) 528
CseI GACGC 2 cut(s) 237, 405
Csp6I GTAC 1 cut(s) 219
CviAII CATG 2 cut(s) 174, 209
CviJI RGCY 6 cut(s) 100, 231, 292, 380, 433, 474
CviKI_1 RGCY 6 cut(s) 100, 231, 292, 380, 433, 474
CviQI GTAC 1 cut(s) 219
DdeI CTNAG 1 cut(s) 486
DpnI GATC 4 cut(s) 141, 195, 357, 510
DpnII GATC 4 cut(s) 139, 193, 355, 508
Eco130I CCWWGG 1 cut(s) 381
Eco47I GGWCC 1 cut(s) 425
Eco57I CTGAAG 1 cut(s) 123
EcoRII CCWGG 1 cut(s) 469
EcoT14I CCWWGG 1 cut(s) 381
EcoT22I ATGCAT 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 381
FaeI CATG 2 cut(s) 177, 212
FaiI YATR 7 cut(s) 78, 80, 175, 210, 499, 503, 532
FaqI GGGAC 1 cut(s) 174
FatI CATG 2 cut(s) 173, 208
FauI CCCGC 1 cut(s) 302
FauNDI CATATG 1 cut(s) 78
FblI GTMKAC 2 cut(s) 135, 502
Fnu4HI GCNGC 5 cut(s) 40, 43, 229, 290, 528
FokI GGATG 2 cut(s) 64, 468
Fsp4HI GCNGC 5 cut(s) 40, 43, 229, 290, 528
FspBI CTAG 1 cut(s) 186
GlaI GCGC 2 cut(s) 38, 46
GluI GCNGC 5 cut(s) 40, 43, 229, 290, 528
GsuI CTGGAG 1 cut(s) 217
HaeII RGCGCY 1 cut(s) 40
HaeIII GGCC 2 cut(s) 380, 433
HapII CCGG 1 cut(s) 293
HgaI GACGC 2 cut(s) 237, 405
HhaI GCGC 2 cut(s) 39, 47
Hin1I GRCGYC 2 cut(s) 129, 248
Hin1II CATG 2 cut(s) 177, 212
Hin6I GCGC 2 cut(s) 37, 45
HinP1I GCGC 2 cut(s) 37, 45
HinfI GANTC 2 cut(s) 164, 478
HpaII CCGG 1 cut(s) 293
HphI GGTGA 1 cut(s) 137
Hpy166II GTNNAC 3 cut(s) 136, 425, 503
Hpy188I TCNGA 4 cut(s) 160, 169, 283, 360
Hpy188III TCNNGA 3 cut(s) 191, 209, 234
Hpy8I GTNNAC 3 cut(s) 136, 425, 503
Hpy99I CGWCG 3 cut(s) 131, 134, 253
HpyCH4III ACNGT 2 cut(s) 20, 444
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 6 cut(s) 23, 173, 242, 349, 420, 451
HpyF10VI GCNNNNNNNGC 1 cut(s) 330
HpyF3I CTNAG 1 cut(s) 486
HpySE526I ACGT 1 cut(s) 129
Hsp92I GRCGYC 2 cut(s) 129, 248
Hsp92II CATG 2 cut(s) 177, 212
HspAI GCGC 2 cut(s) 37, 45
KspI CCGCGG 1 cut(s) 528
Kzo9I GATC 4 cut(s) 139, 193, 355, 508
LmnI GCTCC 1 cut(s) 236
Lsp1109I GCAGC 2 cut(s) 240, 301
LweI GCATC 2 cut(s) 160, 408
MaeI CTAG 1 cut(s) 186
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 3 cut(s) 263, 298, 515
MalI GATC 4 cut(s) 141, 195, 357, 510
MboI GATC 4 cut(s) 139, 193, 355, 508
MboII GAAGA 4 cut(s) 26, 116, 173, 448
MluCI AATT 1 cut(s) 452
MlyI GAGTC 1 cut(s) 472
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 9 cut(s) 78, 128, 159, 162, 213, 279, 370, 423, 495
Mph1103I ATGCAT 1 cut(s) 175
MslI CAYNNNNRTG 2 cut(s) 120, 213
MspA1I CMGCKG 1 cut(s) 527
MspI CCGG 1 cut(s) 293
MspR9I CCNGG 1 cut(s) 471
MvaI CCWGG 1 cut(s) 471
MvnI CGCG 3 cut(s) 45, 507, 527
MwoI GCNNNNNNNGC 1 cut(s) 330
NdeI CATATG 1 cut(s) 78
NdeII GATC 4 cut(s) 139, 193, 355, 508
NlaIII CATG 2 cut(s) 177, 212
NlaIV GGNNCC 2 cut(s) 7, 92
NmuCI GTSAC 2 cut(s) 298, 515
NsiI ATGCAT 1 cut(s) 175
NspI RCATGY 1 cut(s) 177
PagI TCATGA 1 cut(s) 208
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 135
PfeI GAWTC 1 cut(s) 164
PflFI GACNNNGTC 1 cut(s) 128
PkrI GCNGC 5 cut(s) 41, 44, 230, 291, 529
PleI GAGTC 1 cut(s) 472
PpsI GAGTC 1 cut(s) 472
Psp6I CCWGG 1 cut(s) 469
PspGI CCWGG 1 cut(s) 469
PspN4I GGNNCC 2 cut(s) 7, 92
PspPI GGNCC 1 cut(s) 425
PstI CTGCAG 1 cut(s) 351
PsyI GACNNNGTC 1 cut(s) 128
RsaI GTAC 1 cut(s) 220
RsaNI GTAC 1 cut(s) 219
RseI CAYNNNNRTG 2 cut(s) 120, 213
SacII CCGCGG 1 cut(s) 528
SatI GCNGC 5 cut(s) 40, 43, 229, 290, 528
Sau3AI GATC 4 cut(s) 139, 193, 355, 508
Sau96I GGNCC 1 cut(s) 425
SchI GAGTC 1 cut(s) 472
ScrFI CCNGG 1 cut(s) 471
SetI ASST 6 cut(s) 92, 120, 132, 233, 241, 387
SfaNI GCATC 2 cut(s) 160, 408
SfcI CTRYAG 1 cut(s) 347
Sfr303I CCGCGG 1 cut(s) 528
SgrBI CCGCGG 1 cut(s) 528
SinI GGWCC 1 cut(s) 425
SmiMI CAYNNNNRTG 2 cut(s) 120, 213
SpeI ACTAGT 1 cut(s) 185
Sse9I AATT 1 cut(s) 452
SsiI CCGC 6 cut(s) 40, 43, 309, 520, 525, 527
SspMI CTAG 1 cut(s) 186
StyD4I CCNGG 1 cut(s) 469
StyI CCWWGG 1 cut(s) 381
TaaI ACNGT 2 cut(s) 20, 444
TaiI ACGT 1 cut(s) 132
TaqI TCGA 2 cut(s) 120, 258
TaqII GACCGA 1 cut(s) 442
TasI AATT 1 cut(s) 452
TauI GCSGC 3 cut(s) 42, 45, 530
TfiI GAWTC 1 cut(s) 164
TscAI CASTG 3 cut(s) 25, 427, 520
TseFI GTSAC 2 cut(s) 298, 515
TseI GCWGC 2 cut(s) 228, 289
Tsp45I GTSAC 2 cut(s) 298, 515
TspDTI ATGAA 1 cut(s) 225
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 3 cut(s) 25, 427, 520
Tth111I GACNNNGTC 1 cut(s) 128
VpaK11BI GGWCC 1 cut(s) 425
XceI RCATGY 1 cut(s) 177
XmiI GTMKAC 2 cut(s) 135, 502
XspI CTAG 1 cut(s) 186
ZraI GACGTC 1 cut(s) 130
Zsp2I ATGCAT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.