Rh4BG418800

ER-associated misfolded protein catabolic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0017190
Physical Location & Seq
Forward (+)
56540566 .. 56541512
947 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG418800.1

Sequence Viewer

Length: 507 bp
ATGGGTTCCAACGAAGACAGTGGAAAACCACCGAAGCGCCGCCGTGCACGGATGAGTGTCATTTTGCCTCAACCCCATATGATTTTCAGGTTCCCAAAGCCTGAAGACTACGACCACCTCGATGACGAAATAGACGATCATCACCCTCCTCTCTCCGAAGAATCCGATGCATGTTTGGAAACTAGTCCCGATCACAATCCTCGTCATGAAAATGGTACTACCAGCAGCTCCAGACCTTCAACGGCGTCGTATTTCGATTGTAACTTGTGCTTCAAATCGGAGAGGCAGCCGGTTGTGACTGCGTGCGGGCATTTGTATTGCTACTCTTGCTTGTGCGACTGGCTGCAGTTTGGTCGGAAATGTCCTGTTTGTGAGGCCAAGGTGGTCGACGGACGCATCTTTCCGATTTATCAATGCAGTGGACCGAGACCGAAGACACAATTGGTGCAAGTGAGTACCAGTAAGAGTGAGCTAGAGAAAGATCGATTTTGTGGAGTATTCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4 zf-C3HC4_3

Protein Analysis

168

Amino Acids

19.1

Weight (kDa)

6.15

Isoelectric Point (pI)

66.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 86 - 128 1.2e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 86 - 124 9.2e-08 Ring finger domain
zf-C3HC4_2 PF13923 87 - 124 9.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 87 - 124 1.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 87 - 125 1.7e-06 zinc-RING finger domain
zf-RING_UBOX PF13445 87 - 122 8.1e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017190)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74990
fragaria_vesca FvH4_4g34391
malus_domestica MD02G1215900.v1.1
prunus_persica Prupe.8G036400_v2.0.a1
pyrus_communis pycom07g09040
rosa_chinensis RchiOBHm_Chr4g0443821
rosa_laevigata RLG00000005885
rosa_roxburghii Rroxscaffold_5G00384390
rosa_rugosa Rorug04G0347600
rosa_samantha Rh4AG407800 Rh4BG418800 Rh4CG432900 Rh4DG414000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 387
AciI CCGC 2 cut(s) 40, 306
AcuI CTGAAG 1 cut(s) 123
AcyI GRCGYC 1 cut(s) 245
AfaI GTAC 2 cut(s) 217, 457
AgsI TTSAA 2 cut(s) 240, 274
AhlI ACTAGT 1 cut(s) 182
AjuI GAANNNNNNNTTGG 2 cut(s) 425, 457
AluBI AGCT 2 cut(s) 228, 472
AluI AGCT 2 cut(s) 228, 472
Alw21I GWGCWC 1 cut(s) 49
Alw26I GTCTC 1 cut(s) 421
Alw44I GTGCAC 1 cut(s) 45
AoxI GGCC 1 cut(s) 375
ApaLI GTGCAC 1 cut(s) 45
ApeKI GCWGC 3 cut(s) 225, 286, 343
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 1 cut(s) 422
AsuHPI GGTGA 1 cut(s) 134
AvaII GGWCC 1 cut(s) 422
BaeGI GKGCMC 1 cut(s) 49
BbsI GAAGAC 3 cut(s) 21, 111, 440
Bbv12I GWGCWC 1 cut(s) 49
BbvI GCAGC 3 cut(s) 237, 298, 330
BceAI ACGGC 2 cut(s) 27, 258
BcgI CGANNNNNNTGC 2 cut(s) 335, 369
BcoDI GTCTC 1 cut(s) 421
BcuI ACTAGT 1 cut(s) 182
BfaI CTAG 2 cut(s) 183, 473
BfmI CTRYAG 1 cut(s) 344
BfoI RGCGCY 1 cut(s) 40
BisI GCNGC 4 cut(s) 40, 226, 287, 344
BlsI GCNGC 4 cut(s) 41, 227, 288, 345
Bme18I GGWCC 1 cut(s) 422
BmgT120I GGNCC 1 cut(s) 422
BmiI GGNNCC 2 cut(s) 7, 92
BmsI GCATC 2 cut(s) 157, 405
BpiI GAAGAC 3 cut(s) 21, 111, 440
BpmI CTGGAG 1 cut(s) 214
Bsa29I ATCGAT 1 cut(s) 484
BsaBI GATNNNNATC 1 cut(s) 195
BsaHI GRCGYC 1 cut(s) 245
BsaI GGTCTC 1 cut(s) 421
BsaJI CCNNGG 1 cut(s) 378
BsaXI ACNNNNNCTCC 1 cut(s) 486
Bse118I RCCGGY 1 cut(s) 289
Bse1I ACTGG 2 cut(s) 344, 459
Bse8I GATNNNNATC 1 cut(s) 195
BseCI ATCGAT 1 cut(s) 484
BseDI CCNNGG 1 cut(s) 378
BseGI GGATG 1 cut(s) 57
BseJI GATNNNNATC 1 cut(s) 195
BseNI ACTGG 2 cut(s) 344, 459
BseRI GAGGAG 1 cut(s) 138
BseSI GKGCMC 1 cut(s) 49
BseXI GCAGC 3 cut(s) 237, 298, 330
BshFI GGCC 1 cut(s) 377
BshVI ATCGAT 1 cut(s) 484
BsiHKAI GWGCWC 1 cut(s) 49
BsiSI CCGG 1 cut(s) 290
BslFI GGGAC 1 cut(s) 171
BsmAI GTCTC 1 cut(s) 421
BsmFI GGGAC 1 cut(s) 171
BsnI GGCC 1 cut(s) 377
Bso31I GGTCTC 1 cut(s) 421
Bsp1286I GDGCHC 1 cut(s) 49
Bsp143I GATC 3 cut(s) 136, 190, 481
BspACI CCGC 2 cut(s) 40, 306
BspANI GGCC 1 cut(s) 377
BspDI ATCGAT 1 cut(s) 484
BspHI TCATGA 1 cut(s) 205
BspLI GGNNCC 2 cut(s) 7, 92
BspMAI CTGCAG 1 cut(s) 348
BspTNI GGTCTC 1 cut(s) 421
BsrFI RCCGGY 1 cut(s) 289
BsrI ACTGG 2 cut(s) 344, 459
BssAI RCCGGY 1 cut(s) 289
BssECI CCNNGG 1 cut(s) 378
BssMI GATC 3 cut(s) 136, 190, 481
BssNI GRCGYC 1 cut(s) 245
BssT1I CCWWGG 1 cut(s) 378
Bst4CI ACNGT 1 cut(s) 20
BstACI GRCGYC 1 cut(s) 245
BstC8I GCNNGC 2 cut(s) 304, 308
BstF5I GGATG 1 cut(s) 57
BstH2I RGCGCY 1 cut(s) 40
BstHHI GCGC 1 cut(s) 39
BstKTI GATC 3 cut(s) 139, 193, 484
BstMAI GTCTC 1 cut(s) 421
BstMBI GATC 3 cut(s) 136, 190, 481
BstMWI GCNNNNNNNGC 1 cut(s) 327
BstNSI RCATGY 1 cut(s) 174
BstSFI CTRYAG 1 cut(s) 344
BstSLI GKGCMC 1 cut(s) 49
BstV1I GCAGC 3 cut(s) 237, 298, 330
BstV2I GAAGAC 3 cut(s) 21, 111, 440
Bsu15I ATCGAT 1 cut(s) 484
BsuRI GGCC 1 cut(s) 377
BsuTUI ATCGAT 1 cut(s) 484
BtsCI GGATG 1 cut(s) 57
BtsI GCAGTG 1 cut(s) 424
BtsIMutI CAGTG 2 cut(s) 25, 424
Cac8I GCNNGC 2 cut(s) 304, 308
CciI TCATGA 1 cut(s) 205
CfoI GCGC 1 cut(s) 39
Cfr10I RCCGGY 1 cut(s) 289
Cfr13I GGNCC 1 cut(s) 422
ClaI ATCGAT 1 cut(s) 484
CseI GACGC 2 cut(s) 234, 402
Csp6I GTAC 2 cut(s) 216, 456
CviAII CATG 2 cut(s) 171, 206
CviJI RGCY 6 cut(s) 100, 228, 289, 343, 377, 472
CviKI_1 RGCY 6 cut(s) 100, 228, 289, 343, 377, 472
CviQI GTAC 2 cut(s) 216, 456
DpnI GATC 3 cut(s) 138, 192, 483
DpnII GATC 3 cut(s) 136, 190, 481
Eco130I CCWWGG 1 cut(s) 378
Eco31I GGTCTC 1 cut(s) 421
Eco47I GGWCC 1 cut(s) 422
Eco57I CTGAAG 1 cut(s) 123
EcoT14I CCWWGG 1 cut(s) 378
EcoT22I ATGCAT 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 378
FaeI CATG 2 cut(s) 174, 209
FaiI YATR 4 cut(s) 78, 80, 172, 207
FaqI GGGAC 1 cut(s) 171
FatI CATG 2 cut(s) 170, 205
FauI CCCGC 1 cut(s) 299
FauNDI CATATG 1 cut(s) 78
FblI GTMKAC 1 cut(s) 387
Fnu4HI GCNGC 4 cut(s) 40, 226, 287, 344
FokI GGATG 1 cut(s) 64
Fsp4HI GCNGC 4 cut(s) 40, 226, 287, 344
FspBI CTAG 2 cut(s) 183, 473
GlaI GCGC 1 cut(s) 38
GluI GCNGC 4 cut(s) 40, 226, 287, 344
GsuI CTGGAG 1 cut(s) 214
HaeII RGCGCY 1 cut(s) 40
HaeIII GGCC 1 cut(s) 377
HapII CCGG 1 cut(s) 290
HgaI GACGC 2 cut(s) 234, 402
HhaI GCGC 1 cut(s) 39
Hin1I GRCGYC 1 cut(s) 245
Hin1II CATG 2 cut(s) 174, 209
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 1 cut(s) 161
HpaII CCGG 1 cut(s) 290
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 3 cut(s) 47, 388, 422
Hpy188I TCNGA 5 cut(s) 157, 166, 280, 357, 405
Hpy188III TCNNGA 3 cut(s) 188, 206, 231
Hpy8I GTNNAC 3 cut(s) 47, 388, 422
Hpy99I CGWCG 2 cut(s) 250, 392
HpyAV CCTTC 1 cut(s) 246
HpyCH4III ACNGT 1 cut(s) 20
HpyCH4V TGCA 5 cut(s) 47, 170, 346, 417, 448
HpyF10VI GCNNNNNNNGC 1 cut(s) 327
Hsp92I GRCGYC 1 cut(s) 245
Hsp92II CATG 2 cut(s) 174, 209
HspAI GCGC 1 cut(s) 37
Kzo9I GATC 3 cut(s) 136, 190, 481
LmnI GCTCC 1 cut(s) 233
LpnPI CCDG 8 cut(s) 73, 114, 235, 244, 303, 325, 378, 472
Lsp1109I GCAGC 3 cut(s) 237, 298, 330
LweI GCATC 2 cut(s) 157, 405
MaeI CTAG 2 cut(s) 183, 473
MaeIII GTNAC 2 cut(s) 260, 295
MalI GATC 3 cut(s) 138, 192, 483
MboI GATC 3 cut(s) 136, 190, 481
MboII GAAGA 4 cut(s) 26, 116, 170, 445
MfeI CAATTG 1 cut(s) 440
MhlI GDGCHC 1 cut(s) 49
MluCI AATT 1 cut(s) 440
MmeI TCCRAC 2 cut(s) 33, 335
MnlI CCTC 7 cut(s) 78, 128, 156, 159, 210, 276, 367
Mph1103I ATGCAT 1 cut(s) 172
MslI CAYNNNNRTG 2 cut(s) 120, 210
MspI CCGG 1 cut(s) 290
MunI CAATTG 1 cut(s) 440
MwoI GCNNNNNNNGC 1 cut(s) 327
NdeI CATATG 1 cut(s) 78
NdeII GATC 3 cut(s) 136, 190, 481
NlaIII CATG 2 cut(s) 174, 209
NlaIV GGNNCC 2 cut(s) 7, 92
NmuCI GTSAC 1 cut(s) 295
NsiI ATGCAT 1 cut(s) 172
NspI RCATGY 1 cut(s) 174
PagI TCATGA 1 cut(s) 205
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 132
PfeI GAWTC 1 cut(s) 161
PkrI GCNGC 4 cut(s) 41, 227, 288, 345
PspN4I GGNNCC 2 cut(s) 7, 92
PspPI GGNCC 1 cut(s) 422
PstI CTGCAG 1 cut(s) 348
RsaI GTAC 2 cut(s) 217, 457
RsaNI GTAC 2 cut(s) 216, 456
RseI CAYNNNNRTG 2 cut(s) 120, 210
SalI GTCGAC 1 cut(s) 386
SatI GCNGC 4 cut(s) 40, 226, 287, 344
Sau3AI GATC 3 cut(s) 136, 190, 481
Sau96I GGNCC 1 cut(s) 422
SduI GDGCHC 1 cut(s) 49
SetI ASST 6 cut(s) 92, 120, 230, 238, 384, 474
SfaNI GCATC 2 cut(s) 157, 405
SfcI CTRYAG 1 cut(s) 344
SinI GGWCC 1 cut(s) 422
SmiMI CAYNNNNRTG 2 cut(s) 120, 210
SpeI ACTAGT 1 cut(s) 182
Sse9I AATT 1 cut(s) 440
SsiI CCGC 2 cut(s) 40, 306
SspMI CTAG 2 cut(s) 183, 473
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 1 cut(s) 20
TaqI TCGA 4 cut(s) 120, 255, 387, 484
TaqII GACCGA 2 cut(s) 439, 445
TasI AATT 1 cut(s) 440
TauI GCSGC 1 cut(s) 42
TfiI GAWTC 1 cut(s) 161
TscAI CASTG 2 cut(s) 25, 424
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 3 cut(s) 225, 286, 343
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 1 cut(s) 222
TspGWI ACGGA 2 cut(s) 64, 405
TspRI CASTG 2 cut(s) 25, 424
VneI GTGCAC 1 cut(s) 45
VpaK11BI GGWCC 1 cut(s) 422
XceI RCATGY 1 cut(s) 174
XmiI GTMKAC 1 cut(s) 387
XspI CTAG 2 cut(s) 183, 473
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.