Rroxscaffold_5G00384390

ER-associated misfolded protein catabolic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0017190
Physical Location & Seq
Forward (+)
63788819 .. 63789268
450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00384390.1

Sequence Viewer

Length: 450 bp
ATGGGTTCCAACGAAGACAGTGGAAAACCACCGAAGCGCCGCCGTGCACGGATGAGTGTCATTTTGCCTCAATCTCATATGATTTTCAGGTTCCCAAAACCTGAAGACTACGACCACCTCGATGACGACGTAGACGATCATCAGCCTCCTCTCTCCGAAGAATCCGATGCATGTTTGAAAACTAGTCCCGATCACAATCCTCGTCATGAAAATGGTACTACCAGCAGCTCCAGACCTGCAACGGCGTCGTATTTCGATTGTAACTTGTGCTTCAAATCGGAGAGGCAGCCGGTTGTGACTGCGTGCGGGCATTTGTATTGCTACTCTTGCTTGTGCGACTGGCTGCAGTTTGGTCGGAAATGTCCTGTTTGTGAGGCCAAGGTGGTTGAAGGACGTGTCTTTCCGATTTATCAATGCAGTGGGCCGAGACCGAAGACACAATTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4 zf-C3HC4_3

Protein Analysis

149

Amino Acids

16.89

Weight (kDa)

6.57

Isoelectric Point (pI)

74.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 86 - 128 1.2e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 86 - 124 7.4e-08 Ring finger domain
zf-C3HC4_2 PF13923 87 - 124 7.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 87 - 124 1.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 87 - 125 1.4e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017190)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74990
fragaria_vesca FvH4_4g34391
malus_domestica MD02G1215900.v1.1
prunus_persica Prupe.8G036400_v2.0.a1
pyrus_communis pycom07g09040
rosa_chinensis RchiOBHm_Chr4g0443821
rosa_laevigata RLG00000005885
rosa_roxburghii Rroxscaffold_5G00384390
rosa_rugosa Rorug04G0347600
rosa_samantha Rh4AG407800 Rh4BG418800 Rh4CG432900 Rh4DG414000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 244
AccI GTMKAC 1 cut(s) 132
AciI CCGC 2 cut(s) 40, 306
AcuI CTGAAG 1 cut(s) 123
AcyI GRCGYC 1 cut(s) 245
AfaI GTAC 1 cut(s) 217
AflIII ACRYGT 1 cut(s) 394
AgsI TTSAA 3 cut(s) 178, 274, 389
AhlI ACTAGT 1 cut(s) 182
AjiI CACGTC 1 cut(s) 395
AjuI GAANNNNNNNTTGG 1 cut(s) 425
AluBI AGCT 1 cut(s) 228
AluI AGCT 1 cut(s) 228
Alw21I GWGCWC 1 cut(s) 49
Alw26I GTCTC 1 cut(s) 421
Alw44I GTGCAC 1 cut(s) 45
AoxI GGCC 2 cut(s) 375, 422
ApaLI GTGCAC 1 cut(s) 45
ApeKI GCWGC 3 cut(s) 225, 286, 343
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 1 cut(s) 422
BaeGI GKGCMC 1 cut(s) 49
BbsI GAAGAC 3 cut(s) 21, 111, 440
Bbv12I GWGCWC 1 cut(s) 49
BbvI GCAGC 3 cut(s) 237, 298, 330
BceAI ACGGC 2 cut(s) 27, 258
BcgI CGANNNNNNTGC 4 cut(s) 228, 262, 335, 369
BcoDI GTCTC 1 cut(s) 421
BcuI ACTAGT 1 cut(s) 182
BfaI CTAG 1 cut(s) 183
BfmI CTRYAG 1 cut(s) 344
BfoI RGCGCY 1 cut(s) 40
BfuAI ACCTGC 1 cut(s) 244
BisI GCNGC 4 cut(s) 40, 226, 287, 344
BlsI GCNGC 4 cut(s) 41, 227, 288, 345
BmgBI CACGTC 1 cut(s) 395
BmgT120I GGNCC 1 cut(s) 422
BmiI GGNNCC 2 cut(s) 7, 92
BmsI GCATC 1 cut(s) 157
BpiI GAAGAC 3 cut(s) 21, 111, 440
BpmI CTGGAG 1 cut(s) 214
BsaBI GATNNNNATC 1 cut(s) 195
BsaHI GRCGYC 1 cut(s) 245
BsaI GGTCTC 1 cut(s) 421
BsaJI CCNNGG 1 cut(s) 378
Bse118I RCCGGY 1 cut(s) 289
Bse1I ACTGG 1 cut(s) 344
Bse8I GATNNNNATC 1 cut(s) 195
BseDI CCNNGG 1 cut(s) 378
BseGI GGATG 1 cut(s) 57
BseJI GATNNNNATC 1 cut(s) 195
BseNI ACTGG 1 cut(s) 344
BseRI GAGGAG 1 cut(s) 138
BseSI GKGCMC 1 cut(s) 49
BseXI GCAGC 3 cut(s) 237, 298, 330
BshFI GGCC 2 cut(s) 377, 424
BsiHKAI GWGCWC 1 cut(s) 49
BsiSI CCGG 1 cut(s) 290
BslFI GGGAC 1 cut(s) 171
BsmAI GTCTC 1 cut(s) 421
BsmFI GGGAC 1 cut(s) 171
BsnI GGCC 2 cut(s) 377, 424
Bso31I GGTCTC 1 cut(s) 421
Bsp1286I GDGCHC 1 cut(s) 49
Bsp143I GATC 2 cut(s) 136, 190
BspACI CCGC 2 cut(s) 40, 306
BspANI GGCC 2 cut(s) 377, 424
BspHI TCATGA 1 cut(s) 205
BspLI GGNNCC 2 cut(s) 7, 92
BspMAI CTGCAG 1 cut(s) 348
BspMI ACCTGC 1 cut(s) 244
BspTNI GGTCTC 1 cut(s) 421
BsrFI RCCGGY 1 cut(s) 289
BsrI ACTGG 1 cut(s) 344
BssAI RCCGGY 1 cut(s) 289
BssECI CCNNGG 1 cut(s) 378
BssMI GATC 2 cut(s) 136, 190
BssNI GRCGYC 1 cut(s) 245
BssT1I CCWWGG 1 cut(s) 378
Bst4CI ACNGT 1 cut(s) 20
BstACI GRCGYC 1 cut(s) 245
BstC8I GCNNGC 2 cut(s) 304, 308
BstF5I GGATG 1 cut(s) 57
BstH2I RGCGCY 1 cut(s) 40
BstHHI GCGC 1 cut(s) 39
BstKTI GATC 2 cut(s) 139, 193
BstMAI GTCTC 1 cut(s) 421
BstMBI GATC 2 cut(s) 136, 190
BstMWI GCNNNNNNNGC 1 cut(s) 327
BstNSI RCATGY 1 cut(s) 174
BstSFI CTRYAG 1 cut(s) 344
BstSLI GKGCMC 1 cut(s) 49
BstV1I GCAGC 3 cut(s) 237, 298, 330
BstV2I GAAGAC 3 cut(s) 21, 111, 440
BsuRI GGCC 2 cut(s) 377, 424
BtrI CACGTC 1 cut(s) 395
BtsCI GGATG 1 cut(s) 57
BtsI GCAGTG 1 cut(s) 424
BtsIMutI CAGTG 2 cut(s) 25, 424
BveI ACCTGC 1 cut(s) 244
Cac8I GCNNGC 2 cut(s) 304, 308
CciI TCATGA 1 cut(s) 205
CfoI GCGC 1 cut(s) 39
Cfr10I RCCGGY 1 cut(s) 289
Cfr13I GGNCC 1 cut(s) 422
CseI GACGC 1 cut(s) 234
Csp6I GTAC 1 cut(s) 216
CviAII CATG 2 cut(s) 171, 206
CviJI RGCY 6 cut(s) 145, 228, 289, 343, 377, 424
CviKI_1 RGCY 6 cut(s) 145, 228, 289, 343, 377, 424
CviQI GTAC 1 cut(s) 216
DpnI GATC 2 cut(s) 138, 192
DpnII GATC 2 cut(s) 136, 190
Eco130I CCWWGG 1 cut(s) 378
Eco31I GGTCTC 1 cut(s) 421
Eco57I CTGAAG 1 cut(s) 123
EcoT14I CCWWGG 1 cut(s) 378
EcoT22I ATGCAT 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 378
FaeI CATG 2 cut(s) 174, 209
FaiI YATR 4 cut(s) 78, 80, 172, 207
FaqI GGGAC 1 cut(s) 171
FatI CATG 2 cut(s) 170, 205
FauI CCCGC 1 cut(s) 299
FauNDI CATATG 1 cut(s) 78
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 4 cut(s) 40, 226, 287, 344
FokI GGATG 1 cut(s) 64
Fsp4HI GCNGC 4 cut(s) 40, 226, 287, 344
FspBI CTAG 1 cut(s) 183
GlaI GCGC 1 cut(s) 38
GluI GCNGC 4 cut(s) 40, 226, 287, 344
GsuI CTGGAG 1 cut(s) 214
HaeII RGCGCY 1 cut(s) 40
HaeIII GGCC 2 cut(s) 377, 424
HapII CCGG 1 cut(s) 290
HgaI GACGC 1 cut(s) 234
HhaI GCGC 1 cut(s) 39
Hin1I GRCGYC 1 cut(s) 245
Hin1II CATG 2 cut(s) 174, 209
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HinfI GANTC 1 cut(s) 161
HpaII CCGG 1 cut(s) 290
Hpy166II GTNNAC 2 cut(s) 47, 133
Hpy188I TCNGA 5 cut(s) 157, 166, 280, 357, 405
Hpy188III TCNNGA 3 cut(s) 188, 206, 231
Hpy8I GTNNAC 2 cut(s) 47, 133
Hpy99I CGWCG 2 cut(s) 131, 250
HpyAV CCTTC 1 cut(s) 383
HpyCH4III ACNGT 1 cut(s) 20
HpyCH4IV ACGT 2 cut(s) 129, 394
HpyCH4V TGCA 5 cut(s) 47, 170, 239, 346, 417
HpyF10VI GCNNNNNNNGC 1 cut(s) 327
HpySE526I ACGT 2 cut(s) 129, 394
Hsp92I GRCGYC 1 cut(s) 245
Hsp92II CATG 2 cut(s) 174, 209
HspAI GCGC 1 cut(s) 37
Kzo9I GATC 2 cut(s) 136, 190
LmnI GCTCC 1 cut(s) 233
LpnPI CCDG 8 cut(s) 73, 114, 235, 244, 249, 303, 325, 378
Lsp1109I GCAGC 3 cut(s) 237, 298, 330
LweI GCATC 1 cut(s) 157
MaeI CTAG 1 cut(s) 183
MaeII ACGT 2 cut(s) 129, 394
MaeIII GTNAC 2 cut(s) 260, 295
MalI GATC 2 cut(s) 138, 192
MboI GATC 2 cut(s) 136, 190
MboII GAAGA 4 cut(s) 26, 116, 170, 445
MfeI CAATTG 1 cut(s) 440
MhlI GDGCHC 1 cut(s) 49
MluCI AATT 1 cut(s) 440
MmeI TCCRAC 2 cut(s) 33, 335
MnlI CCTC 7 cut(s) 78, 128, 156, 159, 210, 276, 367
Mph1103I ATGCAT 1 cut(s) 172
MseI TTAA 1 cut(s) 448
MslI CAYNNNNRTG 2 cut(s) 120, 210
MspI CCGG 1 cut(s) 290
MunI CAATTG 1 cut(s) 440
MwoI GCNNNNNNNGC 1 cut(s) 327
NdeI CATATG 1 cut(s) 78
NdeII GATC 2 cut(s) 136, 190
NlaIII CATG 2 cut(s) 174, 209
NlaIV GGNNCC 2 cut(s) 7, 92
NmeAIII GCCGAG 1 cut(s) 450
NmuCI GTSAC 1 cut(s) 295
NsiI ATGCAT 1 cut(s) 172
NspI RCATGY 1 cut(s) 174
PagI TCATGA 1 cut(s) 205
PcsI WCGNNNNNNNCGW 3 cut(s) 117, 126, 132
PfeI GAWTC 1 cut(s) 161
PkrI GCNGC 4 cut(s) 41, 227, 288, 345
PspN4I GGNNCC 2 cut(s) 7, 92
PspPI GGNCC 1 cut(s) 422
PstI CTGCAG 1 cut(s) 348
RsaI GTAC 1 cut(s) 217
RsaNI GTAC 1 cut(s) 216
RseI CAYNNNNRTG 2 cut(s) 120, 210
SaqAI TTAA 1 cut(s) 448
SatI GCNGC 4 cut(s) 40, 226, 287, 344
Sau3AI GATC 2 cut(s) 136, 190
Sau96I GGNCC 1 cut(s) 422
SduI GDGCHC 1 cut(s) 49
SetI ASST 8 cut(s) 92, 103, 120, 132, 230, 238, 384, 397
SfaNI GCATC 1 cut(s) 157
SfcI CTRYAG 1 cut(s) 344
SmiMI CAYNNNNRTG 2 cut(s) 120, 210
SpeI ACTAGT 1 cut(s) 182
Sse9I AATT 1 cut(s) 440
SsiI CCGC 2 cut(s) 40, 306
SspMI CTAG 1 cut(s) 183
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 1 cut(s) 20
TaiI ACGT 2 cut(s) 132, 397
TaqI TCGA 2 cut(s) 120, 255
TaqII GACCGA 1 cut(s) 445
TasI AATT 1 cut(s) 440
TauI GCSGC 1 cut(s) 42
TfiI GAWTC 1 cut(s) 161
Tru1I TTAA 1 cut(s) 448
Tru9I TTAA 1 cut(s) 448
TscAI CASTG 2 cut(s) 25, 424
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 3 cut(s) 225, 286, 343
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 1 cut(s) 222
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 2 cut(s) 25, 424
VneI GTGCAC 1 cut(s) 45
XceI RCATGY 1 cut(s) 174
XmiI GTMKAC 1 cut(s) 132
XspI CTAG 1 cut(s) 183
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.