Rh4DG414000

ER-associated misfolded protein catabolic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0017190
Physical Location & Seq
Forward (+)
62455760 .. 62456634
875 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG414000.1

Sequence Viewer

Length: 510 bp
ATGGGTTCCAACGAAGACAGTGGAAAACCACCGAAGCGCCGCCGTGCACGGATGAGTGTCATTTTGCCTCAACCCCATATGATTTTCAGGTTCCCAAAGCCTGAAGACTACGACCACCTCGATGACGACGTCGTAGACGATCATCACCCTCCTCTCTCCGAAGAATCCGATGCATGTTTGGAAACTAGTCCCGATCACAATCCTCGTCATGAAAATGGTACTACCAGCAGCTCCAGACCTTCAACGGCGTCGTATTTCGATTGTAACTTGTGCTTCAAATCGGAGAGGCAGCCGGTTGTGACTGCGTGCGGGCATTTGTATTGCTACTCTTGCTTGTGCGACTGGCTGCAGTTTGGTCGGAAATGTCCTGTTTGTGAGGCCAAGGTGGATGACGGACGCATCTTTCCGATTTATCAATGCAGTGGACCGAGACCAAAGACACAATTGGTGCAAGTAAGTACCAGTACGAGTGAGCTAGAGAAAGATCGATTTTGTGGAGTATTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Prok-RING_4 zf-C3HC4 zf-C3HC4_3

Protein Analysis

169

Amino Acids

19.16

Weight (kDa)

5.77

Isoelectric Point (pI)

63.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 87 - 130 1.9e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 87 - 125 9.3e-08 Ring finger domain
zf-C3HC4_2 PF13923 88 - 125 9.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 88 - 125 1.7e-07 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 88 - 134 7e-07 Prokaryotic RING finger family 4
zf-RING_5 PF14634 88 - 126 1.7e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017190)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74990
fragaria_vesca FvH4_4g34391
malus_domestica MD02G1215900.v1.1
prunus_persica Prupe.8G036400_v2.0.a1
pyrus_communis pycom07g09040
rosa_chinensis RchiOBHm_Chr4g0443821
rosa_laevigata RLG00000005885
rosa_roxburghii Rroxscaffold_5G00384390
rosa_rugosa Rorug04G0347600
rosa_samantha Rh4AG407800 Rh4BG418800 Rh4CG432900 Rh4DG414000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 132
AccI GTMKAC 1 cut(s) 135
AciI CCGC 2 cut(s) 40, 309
AcuI CTGAAG 1 cut(s) 123
AcyI GRCGYC 2 cut(s) 129, 248
AfaI GTAC 3 cut(s) 220, 460, 466
AgsI TTSAA 2 cut(s) 243, 277
AhlI ACTAGT 1 cut(s) 185
AluBI AGCT 2 cut(s) 231, 475
AluI AGCT 2 cut(s) 231, 475
Alw21I GWGCWC 1 cut(s) 49
Alw26I GTCTC 1 cut(s) 424
Alw44I GTGCAC 1 cut(s) 45
AoxI GGCC 1 cut(s) 378
ApaLI GTGCAC 1 cut(s) 45
ApeKI GCWGC 3 cut(s) 228, 289, 346
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 1 cut(s) 425
AsuHPI GGTGA 1 cut(s) 137
AvaII GGWCC 1 cut(s) 425
BaeGI GKGCMC 1 cut(s) 49
BbsI GAAGAC 2 cut(s) 21, 111
Bbv12I GWGCWC 1 cut(s) 49
BbvI GCAGC 3 cut(s) 240, 301, 333
BceAI ACGGC 2 cut(s) 27, 261
BcgI CGANNNNNNTGC 2 cut(s) 338, 372
BcoDI GTCTC 1 cut(s) 424
BcuI ACTAGT 1 cut(s) 185
BfaI CTAG 2 cut(s) 186, 476
BfmI CTRYAG 1 cut(s) 347
BfoI RGCGCY 1 cut(s) 40
BisI GCNGC 4 cut(s) 40, 229, 290, 347
BlsI GCNGC 4 cut(s) 41, 230, 291, 348
Bme18I GGWCC 1 cut(s) 425
BmgT120I GGNCC 1 cut(s) 425
BmiI GGNNCC 2 cut(s) 7, 92
BmsI GCATC 2 cut(s) 160, 408
BpiI GAAGAC 2 cut(s) 21, 111
BpmI CTGGAG 1 cut(s) 217
Bsa29I ATCGAT 1 cut(s) 487
BsaBI GATNNNNATC 1 cut(s) 198
BsaHI GRCGYC 2 cut(s) 129, 248
BsaI GGTCTC 1 cut(s) 424
BsaJI CCNNGG 1 cut(s) 381
BsaXI ACNNNNNCTCC 1 cut(s) 489
Bse118I RCCGGY 1 cut(s) 292
Bse1I ACTGG 2 cut(s) 347, 462
Bse8I GATNNNNATC 1 cut(s) 198
BseCI ATCGAT 1 cut(s) 487
BseDI CCNNGG 1 cut(s) 381
BseGI GGATG 2 cut(s) 57, 394
BseJI GATNNNNATC 1 cut(s) 198
BseNI ACTGG 2 cut(s) 347, 462
BseRI GAGGAG 1 cut(s) 141
BseSI GKGCMC 1 cut(s) 49
BseXI GCAGC 3 cut(s) 240, 301, 333
BshFI GGCC 1 cut(s) 380
BshVI ATCGAT 1 cut(s) 487
BsiHKAI GWGCWC 1 cut(s) 49
BsiSI CCGG 1 cut(s) 293
BslFI GGGAC 1 cut(s) 174
BsmAI GTCTC 1 cut(s) 424
BsmFI GGGAC 1 cut(s) 174
BsnI GGCC 1 cut(s) 380
Bso31I GGTCTC 1 cut(s) 424
Bsp1286I GDGCHC 1 cut(s) 49
Bsp143I GATC 3 cut(s) 139, 193, 484
BspACI CCGC 2 cut(s) 40, 309
BspANI GGCC 1 cut(s) 380
BspDI ATCGAT 1 cut(s) 487
BspHI TCATGA 1 cut(s) 208
BspLI GGNNCC 2 cut(s) 7, 92
BspMAI CTGCAG 1 cut(s) 351
BspTNI GGTCTC 1 cut(s) 424
BsrFI RCCGGY 1 cut(s) 292
BsrI ACTGG 2 cut(s) 347, 462
BssAI RCCGGY 1 cut(s) 292
BssECI CCNNGG 1 cut(s) 381
BssMI GATC 3 cut(s) 139, 193, 484
BssNI GRCGYC 2 cut(s) 129, 248
BssT1I CCWWGG 1 cut(s) 381
Bst4CI ACNGT 1 cut(s) 20
BstACI GRCGYC 2 cut(s) 129, 248
BstC8I GCNNGC 2 cut(s) 307, 311
BstF5I GGATG 2 cut(s) 57, 394
BstH2I RGCGCY 1 cut(s) 40
BstHHI GCGC 1 cut(s) 39
BstKTI GATC 3 cut(s) 142, 196, 487
BstMAI GTCTC 1 cut(s) 424
BstMBI GATC 3 cut(s) 139, 193, 484
BstMWI GCNNNNNNNGC 1 cut(s) 330
BstNSI RCATGY 1 cut(s) 177
BstSFI CTRYAG 1 cut(s) 347
BstSLI GKGCMC 1 cut(s) 49
BstV1I GCAGC 3 cut(s) 240, 301, 333
BstV2I GAAGAC 2 cut(s) 21, 111
Bsu15I ATCGAT 1 cut(s) 487
BsuRI GGCC 1 cut(s) 380
BsuTUI ATCGAT 1 cut(s) 487
BtsCI GGATG 2 cut(s) 57, 394
BtsI GCAGTG 1 cut(s) 427
BtsIMutI CAGTG 2 cut(s) 25, 427
Cac8I GCNNGC 2 cut(s) 307, 311
CciI TCATGA 1 cut(s) 208
CfoI GCGC 1 cut(s) 39
Cfr10I RCCGGY 1 cut(s) 292
Cfr13I GGNCC 1 cut(s) 425
ClaI ATCGAT 1 cut(s) 487
CseI GACGC 2 cut(s) 237, 405
Csp6I GTAC 3 cut(s) 219, 459, 465
CviAII CATG 2 cut(s) 174, 209
CviJI RGCY 6 cut(s) 100, 231, 292, 346, 380, 475
CviKI_1 RGCY 6 cut(s) 100, 231, 292, 346, 380, 475
CviQI GTAC 3 cut(s) 219, 459, 465
DpnI GATC 3 cut(s) 141, 195, 486
DpnII GATC 3 cut(s) 139, 193, 484
Eco130I CCWWGG 1 cut(s) 381
Eco31I GGTCTC 1 cut(s) 424
Eco47I GGWCC 1 cut(s) 425
Eco57I CTGAAG 1 cut(s) 123
EcoT14I CCWWGG 1 cut(s) 381
EcoT22I ATGCAT 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 381
FaeI CATG 2 cut(s) 177, 212
FaiI YATR 4 cut(s) 78, 80, 175, 210
FaqI GGGAC 1 cut(s) 174
FatI CATG 2 cut(s) 173, 208
FauI CCCGC 1 cut(s) 302
FauNDI CATATG 1 cut(s) 78
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 4 cut(s) 40, 229, 290, 347
FokI GGATG 2 cut(s) 64, 401
Fsp4HI GCNGC 4 cut(s) 40, 229, 290, 347
FspBI CTAG 2 cut(s) 186, 476
GlaI GCGC 1 cut(s) 38
GluI GCNGC 4 cut(s) 40, 229, 290, 347
GsuI CTGGAG 1 cut(s) 217
HaeII RGCGCY 1 cut(s) 40
HaeIII GGCC 1 cut(s) 380
HapII CCGG 1 cut(s) 293
HgaI GACGC 2 cut(s) 237, 405
HhaI GCGC 1 cut(s) 39
Hin1I GRCGYC 2 cut(s) 129, 248
Hin1II CATG 2 cut(s) 177, 212
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HinfI GANTC 1 cut(s) 164
HpaII CCGG 1 cut(s) 293
HphI GGTGA 1 cut(s) 137
Hpy166II GTNNAC 3 cut(s) 47, 136, 425
Hpy188I TCNGA 5 cut(s) 160, 169, 283, 360, 408
Hpy188III TCNNGA 3 cut(s) 191, 209, 234
Hpy8I GTNNAC 3 cut(s) 47, 136, 425
Hpy99I CGWCG 3 cut(s) 131, 134, 253
HpyAV CCTTC 1 cut(s) 249
HpyCH4III ACNGT 1 cut(s) 20
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 5 cut(s) 47, 173, 349, 420, 451
HpyF10VI GCNNNNNNNGC 1 cut(s) 330
HpySE526I ACGT 1 cut(s) 129
Hsp92I GRCGYC 2 cut(s) 129, 248
Hsp92II CATG 2 cut(s) 177, 212
HspAI GCGC 1 cut(s) 37
Kzo9I GATC 3 cut(s) 139, 193, 484
LmnI GCTCC 1 cut(s) 236
LpnPI CCDG 8 cut(s) 73, 114, 238, 247, 306, 328, 381, 475
Lsp1109I GCAGC 3 cut(s) 240, 301, 333
LweI GCATC 2 cut(s) 160, 408
MaeI CTAG 2 cut(s) 186, 476
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 2 cut(s) 263, 298
MalI GATC 3 cut(s) 141, 195, 486
MboI GATC 3 cut(s) 139, 193, 484
MboII GAAGA 3 cut(s) 26, 116, 173
MfeI CAATTG 1 cut(s) 443
MhlI GDGCHC 1 cut(s) 49
MluCI AATT 1 cut(s) 443
MmeI TCCRAC 2 cut(s) 33, 338
MnlI CCTC 7 cut(s) 78, 128, 159, 162, 213, 279, 370
Mph1103I ATGCAT 1 cut(s) 175
MslI CAYNNNNRTG 2 cut(s) 120, 213
MspI CCGG 1 cut(s) 293
MunI CAATTG 1 cut(s) 443
MwoI GCNNNNNNNGC 1 cut(s) 330
NdeI CATATG 1 cut(s) 78
NdeII GATC 3 cut(s) 139, 193, 484
NlaIII CATG 2 cut(s) 177, 212
NlaIV GGNNCC 2 cut(s) 7, 92
NmuCI GTSAC 1 cut(s) 298
NsiI ATGCAT 1 cut(s) 175
NspI RCATGY 1 cut(s) 177
PagI TCATGA 1 cut(s) 208
PcsI WCGNNNNNNNCGW 3 cut(s) 117, 126, 135
PfeI GAWTC 1 cut(s) 164
PflFI GACNNNGTC 1 cut(s) 128
PkrI GCNGC 4 cut(s) 41, 230, 291, 348
PspN4I GGNNCC 2 cut(s) 7, 92
PspPI GGNCC 1 cut(s) 425
PstI CTGCAG 1 cut(s) 351
PsyI GACNNNGTC 1 cut(s) 128
RsaI GTAC 3 cut(s) 220, 460, 466
RsaNI GTAC 3 cut(s) 219, 459, 465
RseI CAYNNNNRTG 2 cut(s) 120, 213
SatI GCNGC 4 cut(s) 40, 229, 290, 347
Sau3AI GATC 3 cut(s) 139, 193, 484
Sau96I GGNCC 1 cut(s) 425
SduI GDGCHC 1 cut(s) 49
SetI ASST 7 cut(s) 92, 120, 132, 233, 241, 387, 477
SfaNI GCATC 2 cut(s) 160, 408
SfcI CTRYAG 1 cut(s) 347
SinI GGWCC 1 cut(s) 425
SmiMI CAYNNNNRTG 2 cut(s) 120, 213
SpeI ACTAGT 1 cut(s) 185
Sse9I AATT 1 cut(s) 443
SsiI CCGC 2 cut(s) 40, 309
SspMI CTAG 2 cut(s) 186, 476
StyI CCWWGG 1 cut(s) 381
TaaI ACNGT 1 cut(s) 20
TaiI ACGT 1 cut(s) 132
TaqI TCGA 3 cut(s) 120, 258, 487
TaqII GACCGA 1 cut(s) 442
TasI AATT 1 cut(s) 443
TauI GCSGC 1 cut(s) 42
TfiI GAWTC 1 cut(s) 164
TscAI CASTG 2 cut(s) 25, 427
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 3 cut(s) 228, 289, 346
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 1 cut(s) 225
TspGWI ACGGA 2 cut(s) 64, 408
TspRI CASTG 2 cut(s) 25, 427
Tth111I GACNNNGTC 1 cut(s) 128
VneI GTGCAC 1 cut(s) 45
VpaK11BI GGWCC 1 cut(s) 425
XceI RCATGY 1 cut(s) 177
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 2 cut(s) 186, 476
ZraI GACGTC 1 cut(s) 130
Zsp2I ATGCAT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.