FvH4_4g35080

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018033
Physical Location & Seq
Forward (+)
32530375 .. 32531604
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35080.t1

Sequence Viewer

Length: 450 bp
ATGGTAATGGAAGAGCGCTGGGAAGTTGTGGGAGATACTTACTGCAATGCAAATCTTCTCAGAGTTACAGAGCCTGATGGAAGCGACCAACTGTCATTCACCAAGTTCTTGATGCATATCAAGGCCATCATTAGACGATTTGAGGCGCAGCTGCCTAATGCTACTATGGATATTGATGTTGATATGTTATTAACAGAAAAGAACTGGAATGTGGAAGAAAAAGAAGCATCAATCGAATTCGAATATGATGAAGAGTATGATGATGTTGCTGCCATGAAGTCCATTTCCGACGCACTGTCTGGCCTGCAGGTTCCAGTCCAGACTCATTCGTCCTTGATCGACAGAATATTGTTCCAAGCCTCGAATGCATCACGGAATTGCGAGCATGATCATCACAAAGTTTTGCATATGGCGGTGGAAGTCGATCTTTATGTTGCTGAGAAGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

150

Amino Acids

17.12

Weight (kDa)

4.56

Isoelectric Point (pI)

47.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018033)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g35080 FvH4_7g06610
pyrus_communis pycom02g26040
rosa_multiflora Rmu_sc0015511.1_g000016
rosa_roxburghii Rroxscaffold_4G00316090 Rroxscaffold_5G00385190
rosa_rugosa Rorug01G0121900 Rorug04G0354500
rosa_samantha Rh1AG142900 Rh1BG112100
rosa_wichuraiana Rw4G035620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 328
Acc36I ACCTGC 1 cut(s) 298
AciI CCGC 1 cut(s) 413
AcsI RAATTY 1 cut(s) 236
AfeI AGCGCT 1 cut(s) 17
AhdI GACNNNNNGTC 2 cut(s) 91, 295
AluBI AGCT 1 cut(s) 151
AluI AGCT 1 cut(s) 151
AlwNI CAGNNNCTG 1 cut(s) 74
Aor51HI AGCGCT 1 cut(s) 17
AoxI GGCC 2 cut(s) 123, 301
ApeKI GCWGC 3 cut(s) 148, 151, 269
ApoI RAATTY 1 cut(s) 236
AspLEI GCGC 2 cut(s) 18, 148
AsuHPI GGTGA 1 cut(s) 91
AsuII TTCGAA 1 cut(s) 240
BbvI GCAGC 3 cut(s) 138, 160, 256
BccI CCATC 2 cut(s) 71, 134
BclI TGATCA 1 cut(s) 388
BfmI CTRYAG 1 cut(s) 305
BfoI RGCGCY 1 cut(s) 19
BfuAI ACCTGC 1 cut(s) 298
BisI GCNGC 3 cut(s) 149, 152, 270
BlsI GCNGC 3 cut(s) 150, 153, 271
BmeRI GACNNNNNGTC 2 cut(s) 91, 295
BmiI GGNNCC 1 cut(s) 312
BmsI GCATC 3 cut(s) 102, 236, 377
Bpu14I TTCGAA 1 cut(s) 240
BsaBI GATNNNNATC 1 cut(s) 116
Bse1I ACTGG 2 cut(s) 209, 314
Bse3DI GCAATG 1 cut(s) 52
Bse8I GATNNNNATC 1 cut(s) 116
BseJI GATNNNNATC 1 cut(s) 116
BseMI GCAATG 1 cut(s) 52
BseMII CTCAG 2 cut(s) 73, 429
BseNI ACTGG 2 cut(s) 209, 314
BseXI GCAGC 3 cut(s) 138, 160, 256
BseYI CCCAGC 1 cut(s) 18
BshFI GGCC 2 cut(s) 125, 303
BsmI GAATGC 1 cut(s) 370
BsnI GGCC 2 cut(s) 125, 303
Bsp119I TTCGAA 1 cut(s) 240
Bsp143I GATC 3 cut(s) 336, 388, 424
BspACI CCGC 1 cut(s) 413
BspANI GGCC 2 cut(s) 125, 303
BspCNI CTCAG 2 cut(s) 72, 430
BspLI GGNNCC 1 cut(s) 312
BspMAI CTGCAG 1 cut(s) 309
BspMI ACCTGC 1 cut(s) 298
BspQI GCTCTTC 1 cut(s) 6
BspT104I TTCGAA 1 cut(s) 240
BsrDI GCAATG 1 cut(s) 52
BsrI ACTGG 2 cut(s) 209, 314
BssMI GATC 3 cut(s) 336, 388, 424
Bst4CI ACNGT 2 cut(s) 93, 297
Bst6I CTCTTC 2 cut(s) 6, 246
BstBI TTCGAA 1 cut(s) 240
BstC8I GCNNGC 2 cut(s) 305, 383
BstDEI CTNAG 2 cut(s) 59, 438
BstH2I RGCGCY 1 cut(s) 19
BstHHI GCGC 2 cut(s) 18, 148
BstKTI GATC 3 cut(s) 339, 391, 427
BstMBI GATC 3 cut(s) 336, 388, 424
BstMWI GCNNNNNNNGC 1 cut(s) 365
BstSFI CTRYAG 1 cut(s) 305
BstV1I GCAGC 3 cut(s) 138, 160, 256
BsuRI GGCC 2 cut(s) 125, 303
BtsIMutI CAGTG 1 cut(s) 293
BveI ACCTGC 1 cut(s) 298
Cac8I GCNNGC 2 cut(s) 305, 383
CaiI CAGNNNCTG 1 cut(s) 74
CfoI GCGC 2 cut(s) 18, 148
CseI GACGC 1 cut(s) 299
CviAII CATG 2 cut(s) 274, 386
CviJI RGCY 5 cut(s) 73, 125, 151, 303, 359
CviKI_1 RGCY 5 cut(s) 73, 125, 151, 303, 359
DdeI CTNAG 2 cut(s) 59, 438
DpnI GATC 3 cut(s) 338, 390, 426
DpnII GATC 3 cut(s) 336, 388, 424
DrdI GACNNNNNNGTC 1 cut(s) 328
DriI GACNNNNNGTC 2 cut(s) 91, 295
DseDI GACNNNNNNGTC 1 cut(s) 328
Eam1104I CTCTTC 2 cut(s) 6, 246
Eam1105I GACNNNNNGTC 2 cut(s) 91, 295
EarI CTCTTC 2 cut(s) 6, 246
Eco47III AGCGCT 1 cut(s) 17
EcoRI GAATTC 1 cut(s) 236
EcoT22I ATGCAT 2 cut(s) 117, 370
FaeI CATG 2 cut(s) 277, 389
FalI AAGNNNNNCTT 2 cut(s) 411, 443
FatI CATG 2 cut(s) 273, 385
FauNDI CATATG 1 cut(s) 408
FbaI TGATCA 1 cut(s) 388
Fnu4HI GCNGC 3 cut(s) 149, 152, 270
Fsp4HI GCNGC 3 cut(s) 149, 152, 270
GlaI GCGC 2 cut(s) 17, 147
GluI GCNGC 3 cut(s) 149, 152, 270
GsaI CCCAGC 1 cut(s) 22
HaeII RGCGCY 1 cut(s) 19
HaeIII GGCC 2 cut(s) 125, 303
HgaI GACGC 1 cut(s) 299
HhaI GCGC 2 cut(s) 18, 148
Hin1II CATG 2 cut(s) 277, 389
Hin6I GCGC 2 cut(s) 16, 146
HinP1I GCGC 2 cut(s) 16, 146
HinfI GANTC 1 cut(s) 322
HphI GGTGA 1 cut(s) 91
Hpy188I TCNGA 2 cut(s) 62, 289
Hpy188III TCNNGA 2 cut(s) 109, 319
Hpy99I CGWCG 1 cut(s) 293
HpyAV CCTTC 1 cut(s) 436
HpyCH4III ACNGT 2 cut(s) 93, 297
HpyCH4V TGCA 6 cut(s) 45, 50, 115, 307, 368, 406
HpyF10VI GCNNNNNNNGC 1 cut(s) 365
HpyF3I CTNAG 2 cut(s) 59, 438
Hsp92II CATG 2 cut(s) 277, 389
HspAI GCGC 2 cut(s) 16, 146
Ksp22I TGATCA 1 cut(s) 388
Kzo9I GATC 3 cut(s) 336, 388, 424
LguI GCTCTTC 1 cut(s) 6
LpnPI CCDG 8 cut(s) 4, 87, 190, 285, 293, 317, 327, 332
Lsp1109I GCAGC 3 cut(s) 138, 160, 256
LweI GCATC 3 cut(s) 102, 236, 377
MaeIII GTNAC 1 cut(s) 64
MalI GATC 3 cut(s) 338, 390, 426
MboI GATC 3 cut(s) 336, 388, 424
MboII GAAGA 4 cut(s) 23, 47, 227, 263
MluCI AATT 2 cut(s) 236, 376
MlyI GAGTC 1 cut(s) 316
MmeI TCCRAC 1 cut(s) 312
MnlI CCTC 2 cut(s) 136, 370
Mph1103I ATGCAT 2 cut(s) 117, 370
MseI TTAA 1 cut(s) 191
MspA1I CMGCKG 1 cut(s) 151
Mva1269I GAATGC 1 cut(s) 370
MwoI GCNNNNNNNGC 1 cut(s) 365
NdeI CATATG 1 cut(s) 408
NdeII GATC 3 cut(s) 336, 388, 424
NlaIII CATG 2 cut(s) 277, 389
NlaIV GGNNCC 1 cut(s) 312
NsiI ATGCAT 2 cut(s) 117, 370
NspV TTCGAA 1 cut(s) 240
PciSI GCTCTTC 1 cut(s) 6
PctI GAATGC 1 cut(s) 370
PkrI GCNGC 3 cut(s) 150, 153, 271
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
PspFI CCCAGC 1 cut(s) 18
PspN4I GGNNCC 1 cut(s) 312
PstI CTGCAG 1 cut(s) 309
PstNI CAGNNNCTG 1 cut(s) 74
PvuII CAGCTG 1 cut(s) 151
SapI GCTCTTC 1 cut(s) 6
SaqAI TTAA 1 cut(s) 191
SatI GCNGC 3 cut(s) 149, 152, 270
Sau3AI GATC 3 cut(s) 336, 388, 424
SbfI CCTGCAGG 1 cut(s) 309
SchI GAGTC 1 cut(s) 316
SdaI CCTGCAGG 1 cut(s) 309
SetI ASST 3 cut(s) 153, 312, 447
SfaNI GCATC 3 cut(s) 102, 236, 377
SfcI CTRYAG 1 cut(s) 305
SfuI TTCGAA 1 cut(s) 240
Sse8387I CCTGCAGG 1 cut(s) 309
Sse9I AATT 2 cut(s) 236, 376
SsiI CCGC 1 cut(s) 413
SspI AATATT 1 cut(s) 348
TaaI ACNGT 2 cut(s) 93, 297
TaqI TCGA 5 cut(s) 234, 240, 339, 362, 423
TasI AATT 2 cut(s) 236, 376
Tru1I TTAA 1 cut(s) 191
Tru9I TTAA 1 cut(s) 191
TscAI CASTG 1 cut(s) 300
TseI GCWGC 3 cut(s) 148, 151, 269
TspDTI ATGAA 2 cut(s) 264, 290
TspGWI ACGGA 1 cut(s) 388
TspRI CASTG 1 cut(s) 300
XapI RAATTY 1 cut(s) 236
Zsp2I ATGCAT 2 cut(s) 117, 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.