Rw4G035620

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0018033
Physical Location & Seq
Forward (+)
62460264 .. 62469966
9703 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G035620.1

Sequence Viewer

Length: 372 bp
ATGGTCATGGAAGAGCGCTCGGAAGTTGTGGGAGACACTTACTGCAATGCTAGCAACGACTTGTCATGCACCAAGTTCTTGATGCATATCAAGGCCATCATTAGACGATTCAAGGTGCAGCGACCTAATGCTACCATGGATTTAGATGTTGATATGTTATTAACAGCAAAGAATTGGAGTGTGGAAGAGGACGAAGCCACAATCGAGTTCTATTACGACGAAGATTATGATGTTAATGTTGCTGCCATGAAGTCCATTTCCGACGCACTATGTGGCCTGCAGGTTCCATTTGTTCTATCTGGTATTTTCAAGGATCGCAAAGTTTTGCATATGGCAGTGGAAGTCGATCTTTATGTTGCTGAGAAGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

123

Amino Acids

14.07

Weight (kDa)

4.58

Isoelectric Point (pI)

48.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018033)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g35080 FvH4_7g06610
pyrus_communis pycom02g26040
rosa_multiflora Rmu_sc0015511.1_g000016
rosa_roxburghii Rroxscaffold_4G00316090 Rroxscaffold_5G00385190
rosa_rugosa Rorug01G0121900 Rorug04G0354500
rosa_samantha Rh1AG142900 Rh1BG112100
rosa_wichuraiana Rw4G035620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 271
AclWI GGATC 1 cut(s) 321
AdeI CACNNNGTG 1 cut(s) 272
AfeI AGCGCT 1 cut(s) 17
AgsI TTSAA 2 cut(s) 112, 310
Alw26I GTCTC 1 cut(s) 27
AlwI GGATC 1 cut(s) 321
Aor51HI AGCGCT 1 cut(s) 17
AoxI GGCC 2 cut(s) 93, 274
ApeKI GCWGC 2 cut(s) 118, 242
AspLEI GCGC 1 cut(s) 18
AsuNHI GCTAGC 1 cut(s) 50
BbvI GCAGC 2 cut(s) 130, 229
BccI CCATC 1 cut(s) 104
BcoDI GTCTC 1 cut(s) 27
BfaI CTAG 1 cut(s) 51
BfmI CTRYAG 1 cut(s) 278
BfoI RGCGCY 1 cut(s) 19
BfuAI ACCTGC 1 cut(s) 271
BisI GCNGC 2 cut(s) 119, 243
BlsI GCNGC 2 cut(s) 120, 244
BmiI GGNNCC 1 cut(s) 285
BmsI GCATC 1 cut(s) 72
BmtI GCTAGC 1 cut(s) 54
BsaBI GATNNNNATC 1 cut(s) 86
BsaJI CCNNGG 1 cut(s) 135
Bse3DI GCAATG 1 cut(s) 52
Bse8I GATNNNNATC 1 cut(s) 86
BseDI CCNNGG 1 cut(s) 135
BseJI GATNNNNATC 1 cut(s) 86
BseMI GCAATG 1 cut(s) 52
BseMII CTCAG 1 cut(s) 351
BseXI GCAGC 2 cut(s) 130, 229
BsgI GTGCAG 1 cut(s) 137
BshFI GGCC 2 cut(s) 95, 276
BsmAI GTCTC 1 cut(s) 27
BsnI GGCC 2 cut(s) 95, 276
Bsp143I GATC 2 cut(s) 313, 346
Bsp19I CCATGG 1 cut(s) 135
BspANI GGCC 2 cut(s) 95, 276
BspCNI CTCAG 1 cut(s) 352
BspLI GGNNCC 1 cut(s) 285
BspMAI CTGCAG 1 cut(s) 282
BspMI ACCTGC 1 cut(s) 271
BspOI GCTAGC 1 cut(s) 54
BspPI GGATC 1 cut(s) 321
BspQI GCTCTTC 1 cut(s) 6
BsrDI GCAATG 1 cut(s) 52
BssECI CCNNGG 1 cut(s) 135
BssMI GATC 2 cut(s) 313, 346
BssT1I CCWWGG 1 cut(s) 135
Bst6I CTCTTC 2 cut(s) 6, 180
BstC8I GCNNGC 2 cut(s) 52, 278
BstDEI CTNAG 1 cut(s) 360
BstDSI CCRYGG 1 cut(s) 135
BstH2I RGCGCY 1 cut(s) 19
BstHHI GCGC 1 cut(s) 18
BstKTI GATC 2 cut(s) 316, 349
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 2 cut(s) 313, 346
BstMWI GCNNNNNNNGC 1 cut(s) 51
BstSFI CTRYAG 1 cut(s) 278
BstV1I GCAGC 2 cut(s) 130, 229
BsuRI GGCC 2 cut(s) 95, 276
BtgI CCRYGG 1 cut(s) 135
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 1 cut(s) 342
BveI ACCTGC 1 cut(s) 271
Cac8I GCNNGC 2 cut(s) 52, 278
CfoI GCGC 1 cut(s) 18
CseI GACGC 1 cut(s) 272
CviAII CATG 4 cut(s) 7, 66, 136, 247
CviJI RGCY 3 cut(s) 95, 197, 276
CviKI_1 RGCY 3 cut(s) 95, 197, 276
DdeI CTNAG 1 cut(s) 360
DpnI GATC 2 cut(s) 315, 348
DpnII GATC 2 cut(s) 313, 346
DraIII CACNNNGTG 1 cut(s) 272
Eam1104I CTCTTC 2 cut(s) 6, 180
EarI CTCTTC 2 cut(s) 6, 180
Eco130I CCWWGG 1 cut(s) 135
Eco47III AGCGCT 1 cut(s) 17
EcoT14I CCWWGG 1 cut(s) 135
EcoT22I ATGCAT 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 135
FaeI CATG 4 cut(s) 10, 69, 139, 250
FalI AAGNNNNNCTT 2 cut(s) 333, 365
FatI CATG 4 cut(s) 6, 65, 135, 246
FauNDI CATATG 1 cut(s) 330
Fnu4HI GCNGC 2 cut(s) 119, 243
Fsp4HI GCNGC 2 cut(s) 119, 243
FspBI CTAG 1 cut(s) 51
GlaI GCGC 1 cut(s) 17
GluI GCNGC 2 cut(s) 119, 243
HaeII RGCGCY 1 cut(s) 19
HaeIII GGCC 2 cut(s) 95, 276
HgaI GACGC 1 cut(s) 272
HhaI GCGC 1 cut(s) 18
Hin1II CATG 4 cut(s) 10, 69, 139, 250
Hin6I GCGC 1 cut(s) 16
HinP1I GCGC 1 cut(s) 16
HinfI GANTC 1 cut(s) 108
Hpy188I TCNGA 2 cut(s) 22, 262
Hpy188III TCNNGA 1 cut(s) 79
Hpy99I CGWCG 2 cut(s) 221, 266
HpyCH4V TGCA 6 cut(s) 45, 69, 85, 118, 280, 328
HpyF10VI GCNNNNNNNGC 1 cut(s) 51
HpyF3I CTNAG 1 cut(s) 360
Hsp92II CATG 4 cut(s) 10, 69, 139, 250
HspAI GCGC 1 cut(s) 16
Kzo9I GATC 2 cut(s) 313, 346
LguI GCTCTTC 1 cut(s) 6
LpnPI CCDG 3 cut(s) 266, 285, 290
Lsp1109I GCAGC 2 cut(s) 130, 229
LweI GCATC 1 cut(s) 72
MaeI CTAG 1 cut(s) 51
MalI GATC 2 cut(s) 315, 348
MboI GATC 2 cut(s) 313, 346
MboII GAAGA 3 cut(s) 23, 197, 233
MluCI AATT 1 cut(s) 172
MmeI TCCRAC 1 cut(s) 285
MnlI CCTC 1 cut(s) 181
Mph1103I ATGCAT 1 cut(s) 87
MseI TTAA 2 cut(s) 161, 234
MwoI GCNNNNNNNGC 1 cut(s) 51
NcoI CCATGG 1 cut(s) 135
NdeI CATATG 1 cut(s) 330
NdeII GATC 2 cut(s) 313, 346
NheI GCTAGC 1 cut(s) 50
NlaIII CATG 4 cut(s) 10, 69, 139, 250
NlaIV GGNNCC 1 cut(s) 285
NsiI ATGCAT 1 cut(s) 87
PciSI GCTCTTC 1 cut(s) 6
PfeI GAWTC 1 cut(s) 108
PkrI GCNGC 2 cut(s) 120, 244
PspN4I GGNNCC 1 cut(s) 285
PstI CTGCAG 1 cut(s) 282
SapI GCTCTTC 1 cut(s) 6
SaqAI TTAA 2 cut(s) 161, 234
SatI GCNGC 2 cut(s) 119, 243
Sau3AI GATC 2 cut(s) 313, 346
SbfI CCTGCAGG 1 cut(s) 282
SdaI CCTGCAGG 1 cut(s) 282
SetI ASST 3 cut(s) 117, 127, 285
SfaNI GCATC 1 cut(s) 72
SfcI CTRYAG 1 cut(s) 278
Sse8387I CCTGCAGG 1 cut(s) 282
Sse9I AATT 1 cut(s) 172
SspMI CTAG 1 cut(s) 51
StyI CCWWGG 1 cut(s) 135
TaqI TCGA 2 cut(s) 204, 345
TasI AATT 1 cut(s) 172
TfiI GAWTC 1 cut(s) 108
Tru1I TTAA 2 cut(s) 161, 234
Tru9I TTAA 2 cut(s) 161, 234
TscAI CASTG 1 cut(s) 342
TseI GCWGC 2 cut(s) 118, 242
TspDTI ATGAA 1 cut(s) 263
TspRI CASTG 1 cut(s) 342
XspI CTAG 1 cut(s) 51
Zsp2I ATGCAT 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.