pycom02g26040

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0018033
Physical Location & Seq
Reverse (-)
23888445 .. 23889020
576 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g26040.1

Sequence Viewer

Length: 576 bp
ATGGGCGCTGGTGCCATGCCCAGTGAACGTTGGGAAGTGGTGGGTGAAACTTACTGCAACACACGTCTAAGAGGTGTTTTCGTACCTGAAACCGAGTTGCCATGTCCCGTGTTCTTAATCACCATCAACGTCGTCATCAAAAGGCCCCGTATCGATCACCACAACGATGGCAATATATTGTTCGAAGAAGAAAATTCGACCGTAGATGTGAGCGCAGCCACAGTTCTAGTCCGCTACGACAACATGGTGATCGGCATATCCCAAACCCTTTCTCAGCTCCAAGTCCCTCATCTCCACACTCATCCCTCCTCCGTATCCTTTGTTGAGAAAATCGTCTTCGACACCAGTTGTGCCGAGAAGGACTGCTATTCTTCTAGGCCTTGTCCCGCTTTCAAAGTTCTGCATATGGAGACTTGTATCGAGATTTATGTTGCTGTCAAGTACGTACCGGTGCTGGAAGAATTGCTGGGAATGAAGAACGTCATGGACCAAGTTGGCAACCGATGGACTAATTTCCTCATTGCTTTTGAATTTTTTGATTGCCAATGGATGCAATTGGAAGCTTTGACGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_3 zf-RING_2

Protein Analysis

192

Amino Acids

21.75

Weight (kDa)

4.98

Isoelectric Point (pI)

47.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018033)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g35080 FvH4_7g06610
pyrus_communis pycom02g26040
rosa_multiflora Rmu_sc0015511.1_g000016
rosa_roxburghii Rroxscaffold_4G00316090 Rroxscaffold_5G00385190
rosa_rugosa Rorug01G0121900 Rorug04G0354500
rosa_samantha Rh1AG142900 Rh1BG112100
rosa_wichuraiana Rw4G035620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 11
AciI CCGC 2 cut(s) 232, 387
AclI AACGTT 1 cut(s) 28
AcsI RAATTY 2 cut(s) 193, 530
AfaI GTAC 3 cut(s) 84, 443, 447
AflIII ACRYGT 1 cut(s) 62
AgeI ACCGGT 1 cut(s) 448
AgsI TTSAA 2 cut(s) 394, 530
AjiI CACGTC 1 cut(s) 65
AluBI AGCT 2 cut(s) 277, 563
AluI AGCT 2 cut(s) 277, 563
Alw26I GTCTC 1 cut(s) 404
AoxI GGCC 2 cut(s) 143, 377
ApeKI GCWGC 1 cut(s) 215
ApoI RAATTY 2 cut(s) 193, 530
AsiGI ACCGGT 1 cut(s) 448
AspLEI GCGC 2 cut(s) 8, 215
AspS9I GGNCC 2 cut(s) 144, 487
AsuHPI GGTGA 4 cut(s) 56, 112, 149, 259
AsuII TTCGAA 1 cut(s) 183
AvaII GGWCC 1 cut(s) 487
BanI GGYRCC 1 cut(s) 11
BbsI GAAGAC 1 cut(s) 328
BbvI GCAGC 1 cut(s) 227
BccI CCATC 3 cut(s) 131, 161, 498
BciVI GTATCC 1 cut(s) 325
BcoDI GTCTC 1 cut(s) 404
BfaI CTAG 2 cut(s) 227, 375
BfoI RGCGCY 1 cut(s) 9
BfuI GTATCC 1 cut(s) 325
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
Bme18I GGWCC 1 cut(s) 487
BmgBI CACGTC 1 cut(s) 65
BmgT120I GGNCC 2 cut(s) 144, 487
BmiI GGNNCC 2 cut(s) 13, 146
BmrI ACTGGG 1 cut(s) 15
BmsI GCATC 1 cut(s) 540
BmuI ACTGGG 1 cut(s) 15
BpiI GAAGAC 1 cut(s) 328
Bpu14I TTCGAA 1 cut(s) 183
Bsa29I ATCGAT 1 cut(s) 153
BsaAI YACGTR 1 cut(s) 445
BsaWI WCCGGW 1 cut(s) 448
Bse118I RCCGGY 1 cut(s) 448
Bse1I ACTGG 2 cut(s) 21, 345
Bse3DI GCAATG 1 cut(s) 519
BseCI ATCGAT 1 cut(s) 153
BseGI GGATG 2 cut(s) 301, 555
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 1 cut(s) 287
BseNI ACTGG 2 cut(s) 21, 345
BseRI GAGGAG 1 cut(s) 298
BseXI GCAGC 1 cut(s) 227
BseYI CCCAGC 1 cut(s) 466
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 2 cut(s) 145, 379
BshNI GGYRCC 1 cut(s) 11
BshTI ACCGGT 1 cut(s) 448
BshVI ATCGAT 1 cut(s) 153
BsiEI CGRYCG 1 cut(s) 201
BsiSI CCGG 1 cut(s) 449
BslFI GGGAC 3 cut(s) 90, 269, 369
BsmAI GTCTC 1 cut(s) 404
BsmFI GGGAC 3 cut(s) 90, 269, 369
BsnI GGCC 2 cut(s) 145, 379
Bsp119I TTCGAA 1 cut(s) 183
Bsp143I GATC 2 cut(s) 154, 249
BspACI CCGC 2 cut(s) 232, 387
BspANI GGCC 2 cut(s) 145, 379
BspCNI CTCAG 1 cut(s) 286
BspDI ATCGAT 1 cut(s) 153
BspLI GGNNCC 2 cut(s) 13, 146
BspT104I TTCGAA 1 cut(s) 183
BspT107I GGYRCC 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 519
BsrFI RCCGGY 1 cut(s) 448
BsrI ACTGG 2 cut(s) 21, 345
BssAI RCCGGY 1 cut(s) 448
BssMI GATC 2 cut(s) 154, 249
Bst4CI ACNGT 2 cut(s) 202, 223
BstBAI YACGTR 1 cut(s) 445
BstBI TTCGAA 1 cut(s) 183
BstDEI CTNAG 2 cut(s) 68, 273
BstF5I GGATG 2 cut(s) 301, 555
BstH2I RGCGCY 1 cut(s) 9
BstHHI GCGC 2 cut(s) 8, 215
BstKTI GATC 2 cut(s) 157, 252
BstMAI GTCTC 1 cut(s) 404
BstMBI GATC 2 cut(s) 154, 249
BstMCI CGRYCG 1 cut(s) 201
BstSNI TACGTA 1 cut(s) 445
BstV1I GCAGC 1 cut(s) 227
BstV2I GAAGAC 1 cut(s) 328
BstXI CCANNNNNNTGG 1 cut(s) 167
Bsu15I ATCGAT 1 cut(s) 153
BsuI GTATCC 1 cut(s) 325
BsuRI GGCC 2 cut(s) 145, 379
BsuTUI ATCGAT 1 cut(s) 153
BtrI CACGTC 1 cut(s) 65
BtsCI GGATG 2 cut(s) 301, 555
BtsIMutI CAGTG 1 cut(s) 28
CfoI GCGC 2 cut(s) 8, 215
Cfr10I RCCGGY 1 cut(s) 448
Cfr13I GGNCC 2 cut(s) 144, 487
ClaI ATCGAT 1 cut(s) 153
Csp6I GTAC 3 cut(s) 83, 442, 446
CspAI ACCGGT 1 cut(s) 448
CviAII CATG 4 cut(s) 16, 102, 244, 484
CviJI RGCY 5 cut(s) 145, 218, 277, 379, 563
CviKI_1 RGCY 5 cut(s) 145, 218, 277, 379, 563
CviQI GTAC 3 cut(s) 83, 442, 446
DdeI CTNAG 2 cut(s) 68, 273
DpnI GATC 2 cut(s) 156, 251
DpnII GATC 2 cut(s) 154, 249
Eco105I TACGTA 1 cut(s) 445
Eco147I AGGCCT 1 cut(s) 379
Eco47I GGWCC 1 cut(s) 487
EcoO109I RGGNCCY 1 cut(s) 144
FaeI CATG 4 cut(s) 19, 105, 247, 487
FaiI YATR 9 cut(s) 17, 103, 176, 245, 257, 405, 407, 429, 485
FaqI GGGAC 3 cut(s) 90, 269, 369
FatI CATG 4 cut(s) 15, 101, 243, 483
FauI CCCGC 1 cut(s) 394
FauNDI CATATG 1 cut(s) 405
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 2 cut(s) 288, 562
Fsp4HI GCNGC 1 cut(s) 216
FspBI CTAG 2 cut(s) 227, 375
GlaI GCGC 2 cut(s) 7, 214
GluI GCNGC 1 cut(s) 216
GsaI CCCAGC 1 cut(s) 470
HaeII RGCGCY 1 cut(s) 9
HaeIII GGCC 2 cut(s) 145, 379
HapII CCGG 1 cut(s) 449
HhaI GCGC 2 cut(s) 8, 215
Hin1II CATG 4 cut(s) 19, 105, 247, 487
Hin6I GCGC 2 cut(s) 6, 213
HinP1I GCGC 2 cut(s) 6, 213
HindIII AAGCTT 1 cut(s) 561
HpaII CCGG 1 cut(s) 449
HphI GGTGA 4 cut(s) 56, 112, 149, 259
Hpy166II GTNNAC 1 cut(s) 26
Hpy188III TCNNGA 1 cut(s) 421
Hpy8I GTNNAC 1 cut(s) 26
Hpy99I CGWCG 1 cut(s) 134
HpyAV CCTTC 1 cut(s) 352
HpyCH4III ACNGT 2 cut(s) 202, 223
HpyCH4IV ACGT 6 cut(s) 28, 64, 129, 444, 480, 569
HpyCH4V TGCA 3 cut(s) 57, 403, 553
HpyF3I CTNAG 2 cut(s) 68, 273
HpySE526I ACGT 6 cut(s) 28, 64, 129, 444, 480, 569
Hsp92II CATG 4 cut(s) 19, 105, 247, 487
HspAI GCGC 2 cut(s) 6, 213
Kzo9I GATC 2 cut(s) 154, 249
LmnI GCTCC 1 cut(s) 282
LpnPI CCDG 6 cut(s) 34, 99, 358, 440, 452, 462
Lsp1109I GCAGC 1 cut(s) 227
LweI GCATC 1 cut(s) 540
MaeI CTAG 2 cut(s) 227, 375
MaeII ACGT 6 cut(s) 28, 64, 129, 444, 480, 569
MalI GATC 2 cut(s) 156, 251
MboI GATC 2 cut(s) 154, 249
MboII GAAGA 6 cut(s) 197, 200, 328, 363, 470, 487
MfeI CAATTG 1 cut(s) 554
MluCI AATT 5 cut(s) 193, 461, 511, 530, 554
MnlI CCTC 5 cut(s) 65, 297, 316, 319, 527
MseI TTAA 1 cut(s) 116
MslI CAYNNNNRTG 1 cut(s) 165
MspI CCGG 1 cut(s) 449
MunI CAATTG 1 cut(s) 554
NdeI CATATG 1 cut(s) 405
NdeII GATC 2 cut(s) 154, 249
NlaIII CATG 4 cut(s) 19, 105, 247, 487
NlaIV GGNNCC 2 cut(s) 13, 146
NmeAIII GCCGAG 1 cut(s) 379
NspV TTCGAA 1 cut(s) 183
PceI AGGCCT 1 cut(s) 379
PinAI ACCGGT 1 cut(s) 448
PkrI GCNGC 1 cut(s) 217
Ppu21I YACGTR 1 cut(s) 445
Psp1406I AACGTT 1 cut(s) 28
PspFI CCCAGC 1 cut(s) 466
PspN4I GGNNCC 2 cut(s) 13, 146
PspPI GGNCC 2 cut(s) 144, 487
RsaI GTAC 3 cut(s) 84, 443, 447
RsaNI GTAC 3 cut(s) 83, 442, 446
RseI CAYNNNNRTG 1 cut(s) 165
SaqAI TTAA 1 cut(s) 116
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 2 cut(s) 154, 249
Sau96I GGNCC 2 cut(s) 144, 487
SfaNI GCATC 1 cut(s) 540
SfuI TTCGAA 1 cut(s) 183
SinI GGWCC 1 cut(s) 487
SmiMI CAYNNNNRTG 1 cut(s) 165
SnaBI TACGTA 1 cut(s) 445
Sse9I AATT 5 cut(s) 193, 461, 511, 530, 554
SseBI AGGCCT 1 cut(s) 379
SsiI CCGC 2 cut(s) 232, 387
SspMI CTAG 2 cut(s) 227, 375
StuI AGGCCT 1 cut(s) 379
TaaI ACNGT 2 cut(s) 202, 223
TaiI ACGT 6 cut(s) 31, 67, 132, 447, 483, 572
TaqI TCGA 5 cut(s) 153, 183, 197, 339, 420
TasI AATT 5 cut(s) 193, 461, 511, 530, 554
Tru1I TTAA 1 cut(s) 116
Tru9I TTAA 1 cut(s) 116
TscAI CASTG 1 cut(s) 28
TseI GCWGC 1 cut(s) 215
TspDTI ATGAA 1 cut(s) 488
TspGWI ACGGA 1 cut(s) 301
TspRI CASTG 1 cut(s) 28
VpaK11BI GGWCC 1 cut(s) 487
XapI RAATTY 2 cut(s) 193, 530
XspI CTAG 2 cut(s) 227, 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.