Rroxscaffold_4G00316090

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0018033
Physical Location & Seq
Reverse (-)
40739736 .. 40741549
1814 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00316090.1

Sequence Viewer

Length: 639 bp
ATGCAAGAAGCATCGTGGAGATCCAGGAATGTGAGCGATCACCTTGCACGCCTTGCACTACTCATCATGGGTTGGCACCAACGCTATTCTCTTATTGTCTTGCAGGATGTTTTGGACACTGGTGATGACTTGGTCTCACTTGAGCTCACAATATGTTTGACTATTCAAGACAACAACATCTCCATGGCCAATGAACAATGGGTTGTGGTGGGCGACACTTGGTGCATTGCAAAGTTGCACAGAGTCATCGAACCAGAAAAGGTCTTGCCCTGCATCAAGTTCTTAATGCATATTAATGCCACCATTCATCGACTGCAACAACAGCATGGTGACAATACAAGTTTTGATGACAATGACCAGGAAGATCTAATAGACATATTGTTAGAAGAAGAAAACTGGAGCTCTTATGAGAATGACGTCACAATTGAACTCGAGTGTGACGACCCATATGACGTGAACATGGATGCAATATCTAAGGCCCTTTCTCAACTGCAAGTGCCTTTACAAGCTCAGGAATCGTTGATAGACACCATATTGTTCCGGAGCTTCGAAAGTGCAAGAAACTGCAGTTCTTATGTAGATCGCAAGATTTTGCATATGGAGTTCAGTGTAGATCTTTATGTTGTTTCAGTAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

212

Amino Acids

24.44

Weight (kDa)

4.37

Isoelectric Point (pI)

52.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018033)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g35080 FvH4_7g06610
pyrus_communis pycom02g26040
rosa_multiflora Rmu_sc0015511.1_g000016
rosa_roxburghii Rroxscaffold_4G00316090 Rroxscaffold_5G00385190
rosa_rugosa Rorug01G0121900 Rorug04G0354500
rosa_samantha Rh1AG142900 Rh1BG112100
rosa_wichuraiana Rw4G035620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 420
AccB1I GGYRCC 1 cut(s) 75
AccIII TCCGGA 1 cut(s) 540
AclWI GGATC 1 cut(s) 15
AcoI YGGCCR 1 cut(s) 186
AcyI GRCGYC 1 cut(s) 417
AdeI CACNNNGTG 1 cut(s) 222
AgsI TTSAA 2 cut(s) 167, 428
AjiI CACGTC 1 cut(s) 454
AjnI CCWGG 2 cut(s) 23, 357
AluBI AGCT 4 cut(s) 145, 402, 509, 546
AluI AGCT 4 cut(s) 145, 402, 509, 546
Alw21I GWGCWC 2 cut(s) 147, 404
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 1 cut(s) 15
Ama87I CYCGRG 1 cut(s) 431
Aor13HI TCCGGA 1 cut(s) 540
AoxI GGCC 2 cut(s) 186, 477
AseI ATTAAT 1 cut(s) 294
AspS9I GGNCC 1 cut(s) 478
AsuHPI GGTGA 3 cut(s) 32, 134, 341
AsuII TTCGAA 1 cut(s) 549
AvaI CYCGRG 1 cut(s) 431
BalI TGGCCA 1 cut(s) 188
BanI GGYRCC 1 cut(s) 75
BanII GRGCYC 2 cut(s) 147, 404
Bbv12I GWGCWC 2 cut(s) 147, 404
BcgI CGANNNNNNTGC 2 cut(s) 26, 60
BciT130I CCWGG 2 cut(s) 25, 359
BcoDI GTCTC 1 cut(s) 139
BfmI CTRYAG 1 cut(s) 565
BglII AGATCT 2 cut(s) 364, 613
Bme1390I CCNGG 2 cut(s) 25, 359
BmeT110I CYCGRG 1 cut(s) 431
BmgBI CACGTC 1 cut(s) 454
BmgT120I GGNCC 1 cut(s) 478
BmiI GGNNCC 1 cut(s) 77
BmrFI CCNGG 2 cut(s) 25, 359
BmsI GCATC 3 cut(s) 20, 282, 454
BpmI CTGGAG 1 cut(s) 418
Bpu10I CCTNAGC 1 cut(s) 510
Bpu14I TTCGAA 1 cut(s) 549
BpuEI CTTGAG 1 cut(s) 161
BsaHI GRCGYC 1 cut(s) 417
BsaI GGTCTC 1 cut(s) 139
BsaJI CCNNGG 1 cut(s) 183
BsaWI WCCGGW 1 cut(s) 540
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bse1I ACTGG 2 cut(s) 124, 401
Bse3DI GCAATG 1 cut(s) 225
BseAI TCCGGA 1 cut(s) 540
BseBI CCWGG 2 cut(s) 25, 359
BseDI CCNNGG 1 cut(s) 183
BseGI GGATG 2 cut(s) 112, 469
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 1 cut(s) 524
BseNI ACTGG 2 cut(s) 124, 401
BshFI GGCC 2 cut(s) 188, 479
BshNI GGYRCC 1 cut(s) 75
BsiHKAI GWGCWC 2 cut(s) 147, 404
BsiHKCI CYCGRG 1 cut(s) 431
BsiSI CCGG 1 cut(s) 541
BsmAI GTCTC 1 cut(s) 139
BsnI GGCC 2 cut(s) 188, 479
Bso31I GGTCTC 1 cut(s) 139
BsoBI CYCGRG 1 cut(s) 431
Bsp119I TTCGAA 1 cut(s) 549
Bsp1286I GDGCHC 2 cut(s) 147, 404
Bsp13I TCCGGA 1 cut(s) 540
Bsp143I GATC 5 cut(s) 20, 37, 364, 580, 613
Bsp19I CCATGG 1 cut(s) 183
BspANI GGCC 2 cut(s) 188, 479
BspCNI CTCAG 1 cut(s) 523
BspEI TCCGGA 1 cut(s) 540
BspLI GGNNCC 1 cut(s) 77
BspMAI CTGCAG 1 cut(s) 569
BspPI GGATC 1 cut(s) 15
BspT104I TTCGAA 1 cut(s) 549
BspT107I GGYRCC 1 cut(s) 75
BspTNI GGTCTC 1 cut(s) 139
BsrDI GCAATG 1 cut(s) 225
BsrI ACTGG 2 cut(s) 124, 401
BssECI CCNNGG 1 cut(s) 183
BssMI GATC 5 cut(s) 20, 37, 364, 580, 613
BssNI GRCGYC 1 cut(s) 417
BssT1I CCWWGG 1 cut(s) 183
Bst2UI CCWGG 2 cut(s) 25, 359
BstACI GRCGYC 1 cut(s) 417
BstAPI GCANNNNNTGC 1 cut(s) 53
BstBI TTCGAA 1 cut(s) 549
BstC8I GCNNGC 1 cut(s) 49
BstDEI CTNAG 2 cut(s) 474, 510
BstDSI CCRYGG 1 cut(s) 183
BstF5I GGATG 2 cut(s) 112, 469
BstKTI GATC 5 cut(s) 23, 40, 367, 583, 616
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 5 cut(s) 20, 37, 364, 580, 613
BstMWI GCNNNNNNNGC 2 cut(s) 53, 322
BstNI CCWGG 2 cut(s) 25, 359
BstSCI CCNGG 2 cut(s) 23, 357
BstSFI CTRYAG 1 cut(s) 565
BstX2I RGATCY 3 cut(s) 20, 364, 613
BstYI RGATCY 3 cut(s) 20, 364, 613
BsuRI GGCC 2 cut(s) 188, 479
BtgI CCRYGG 1 cut(s) 183
BtrI CACGTC 1 cut(s) 454
BtsCI GGATG 2 cut(s) 112, 469
BtsIMutI CAGTG 2 cut(s) 117, 613
Cac8I GCNNGC 1 cut(s) 49
Cfr13I GGNCC 1 cut(s) 478
CviAII CATG 4 cut(s) 67, 184, 326, 460
CviJI RGCY 6 cut(s) 145, 188, 402, 479, 509, 546
CviKI_1 RGCY 6 cut(s) 145, 188, 402, 479, 509, 546
DdeI CTNAG 2 cut(s) 474, 510
DpnI GATC 5 cut(s) 22, 39, 366, 582, 615
DpnII GATC 5 cut(s) 20, 37, 364, 580, 613
DraIII CACNNNGTG 1 cut(s) 222
EaeI YGGCCR 1 cut(s) 186
Ecl136II GAGCTC 2 cut(s) 145, 402
Eco130I CCWWGG 1 cut(s) 183
Eco24I GRGCYC 2 cut(s) 147, 404
Eco31I GGTCTC 1 cut(s) 139
Eco53kI GAGCTC 2 cut(s) 145, 402
Eco88I CYCGRG 1 cut(s) 431
EcoICRI GAGCTC 2 cut(s) 145, 402
EcoO109I RGGNCCY 1 cut(s) 478
EcoRII CCWGG 2 cut(s) 23, 357
EcoT14I CCWWGG 1 cut(s) 183
EcoT22I ATGCAT 1 cut(s) 291
EcoT38I GRGCYC 2 cut(s) 147, 404
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 4 cut(s) 70, 187, 329, 463
FatI CATG 4 cut(s) 66, 183, 325, 459
FauNDI CATATG 2 cut(s) 448, 597
FokI GGATG 2 cut(s) 119, 476
FriOI GRGCYC 2 cut(s) 147, 404
GsuI CTGGAG 1 cut(s) 418
HaeIII GGCC 2 cut(s) 188, 479
HapII CCGG 1 cut(s) 541
Hin1I GRCGYC 1 cut(s) 417
Hin1II CATG 4 cut(s) 70, 187, 329, 463
HinfI GANTC 2 cut(s) 243, 515
HpaII CCGG 1 cut(s) 541
HphI GGTGA 3 cut(s) 32, 134, 341
Hpy166II GTNNAC 1 cut(s) 457
Hpy188III TCNNGA 3 cut(s) 167, 512, 541
Hpy8I GTNNAC 1 cut(s) 457
HpyCH4IV ACGT 2 cut(s) 417, 453
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 322
HpyF3I CTNAG 2 cut(s) 474, 510
HpySE526I ACGT 2 cut(s) 417, 453
Hsp92I GRCGYC 1 cut(s) 417
Hsp92II CATG 4 cut(s) 70, 187, 329, 463
Kpn2I TCCGGA 1 cut(s) 540
Kzo9I GATC 5 cut(s) 20, 37, 364, 580, 613
LmnI GCTCC 2 cut(s) 399, 543
LweI GCATC 3 cut(s) 20, 282, 454
MaeII ACGT 2 cut(s) 417, 453
MaeIII GTNAC 3 cut(s) 329, 418, 437
MalI GATC 5 cut(s) 22, 39, 366, 582, 615
MboI GATC 5 cut(s) 20, 37, 364, 580, 613
MboII GAAGA 3 cut(s) 374, 398, 401
MfeI CAATTG 1 cut(s) 423
MflI RGATCY 3 cut(s) 20, 364, 613
MhlI GDGCHC 2 cut(s) 147, 404
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 1 cut(s) 423
MluNI TGGCCA 1 cut(s) 188
MlyI GAGTC 1 cut(s) 252
Mox20I TGGCCA 1 cut(s) 188
Mph1103I ATGCAT 1 cut(s) 291
MroI TCCGGA 1 cut(s) 540
MscI TGGCCA 1 cut(s) 188
MseI TTAA 2 cut(s) 284, 294
MslI CAYNNNNRTG 2 cut(s) 182, 294
Msp20I TGGCCA 1 cut(s) 188
MspI CCGG 1 cut(s) 541
MspR9I CCNGG 2 cut(s) 25, 359
MunI CAATTG 1 cut(s) 423
MvaI CCWGG 2 cut(s) 25, 359
MwoI GCNNNNNNNGC 2 cut(s) 53, 322
NcoI CCATGG 1 cut(s) 183
NdeI CATATG 2 cut(s) 448, 597
NdeII GATC 5 cut(s) 20, 37, 364, 580, 613
NlaIII CATG 4 cut(s) 70, 187, 329, 463
NlaIV GGNNCC 1 cut(s) 77
NmuCI GTSAC 3 cut(s) 329, 418, 437
NsiI ATGCAT 1 cut(s) 291
NspV TTCGAA 1 cut(s) 549
PaeR7I CTCGAG 1 cut(s) 431
PcsI WCGNNNNNNNCGW 1 cut(s) 438
PfeI GAWTC 1 cut(s) 515
PflFI GACNNNGTC 1 cut(s) 131
PfoI TCCNGGA 1 cut(s) 23
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
PshBI ATTAAT 1 cut(s) 294
Psp124BI GAGCTC 2 cut(s) 147, 404
Psp6I CCWGG 2 cut(s) 23, 357
PspGI CCWGG 2 cut(s) 23, 357
PspN4I GGNNCC 1 cut(s) 77
PspPI GGNCC 1 cut(s) 478
PspXI VCTCGAGB 1 cut(s) 431
PstI CTGCAG 1 cut(s) 569
PsuI RGATCY 3 cut(s) 20, 364, 613
PsyI GACNNNGTC 1 cut(s) 131
RseI CAYNNNNRTG 2 cut(s) 182, 294
SacI GAGCTC 2 cut(s) 147, 404
SaqAI TTAA 2 cut(s) 284, 294
Sau3AI GATC 5 cut(s) 20, 37, 364, 580, 613
Sau96I GGNCC 1 cut(s) 478
SchI GAGTC 1 cut(s) 252
ScrFI CCNGG 2 cut(s) 25, 359
SduI GDGCHC 2 cut(s) 147, 404
SetI ASST 8 cut(s) 45, 147, 264, 404, 420, 456, 511, 548
SfaNI GCATC 3 cut(s) 20, 282, 454
SfcI CTRYAG 1 cut(s) 565
Sfr274I CTCGAG 1 cut(s) 431
SfuI TTCGAA 1 cut(s) 549
SlaI CTCGAG 1 cut(s) 431
SmiMI CAYNNNNRTG 2 cut(s) 182, 294
SmlI CTYRAG 2 cut(s) 140, 431
SmoI CTYRAG 2 cut(s) 140, 431
Sse9I AATT 1 cut(s) 423
SstI GAGCTC 2 cut(s) 147, 404
StyD4I CCNGG 2 cut(s) 23, 357
StyI CCWWGG 1 cut(s) 183
TaiI ACGT 2 cut(s) 420, 456
TaqI TCGA 4 cut(s) 249, 310, 432, 549
TasI AATT 1 cut(s) 423
TfiI GAWTC 1 cut(s) 515
Tru1I TTAA 2 cut(s) 284, 294
Tru9I TTAA 2 cut(s) 284, 294
TscAI CASTG 2 cut(s) 124, 613
TseFI GTSAC 3 cut(s) 329, 418, 437
Tsp45I GTSAC 3 cut(s) 329, 418, 437
TspDTI ATGAA 2 cut(s) 207, 296
TspRI CASTG 2 cut(s) 124, 613
Tth111I GACNNNGTC 1 cut(s) 131
VspI ATTAAT 1 cut(s) 294
XhoI CTCGAG 1 cut(s) 431
ZraI GACGTC 1 cut(s) 418
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.