FvH4_4g35680

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010265
Physical Location & Seq
Reverse (-)
32908759 .. 32912583
3825 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35680.t2

Sequence Viewer

Length: 1320 bp
ATGGCAACCACGGCGAGTTCCGAAGTCTCCGACGGCCCGGTCCTCAGCCTCATCAACAAGCGCATCCGCGGCCTCCGCAAGAAGCAGAACCGCATCAGCCAGATGGAGGAGTCTCTCGCCCAAGGCAAAACCCTCAACAACGAGCAACTCGAGGTCCTCCGCACCAAGTCCGCCATCGTTGCCTCCATCGACGAGCTCGAGAAGCTTCGCCAGCCTCTCTCCGTCGCCGTCGCCGAGGAAATCAGCCTCGCCGTCCAGGTCACCGCCCCTCCCGCCGCCGCCGCTACTGAGCCCGAAGAACACCACGAAACTCCCCCCAATGCTGTGCAGCAGAGCGGCGAATCCGCCGCCGACGTCGATGAGCAGAACCACCACCAGGCGGCGGTGGAGGAGCTTCTGAACCTGCTCTACTTTGGCTCGTTGTTCGATGTCAAGACGCAGAGTGACTTCACCTCGATTATGTTCACCAGGAGCCACGAGAGAGGGTGCTGCTTGACCTATGACACCGTCACTGATGACGACACTGATATGCTCGCCGAGAAGGACTTGGATTTGCTCTCCTCACTCAGTGGCTTGTTGATCTCCAGGCCGGTGGCTTCGGGCTTATCTCACAAGAATGCTCTGCAGCGCTGCATTGAGCACGCCAAGCTATGGCTCGCTAACTCAGACCAGCCAATTGAGCCTAATTCTAGTGTCACATATGTGAGTTTGAGAGAGAGGCTTAGTAAGATTATGGCTTCGGACTACTTTACCATCACTCCAGAAATGAAAGCTCCGGAGGCAATGGCAGCTGCTGCTGGTGGAAACTTTGCTCCGTTTCAGGTGAGGGGTGTGGATGTGGACAGTTCATTTGTACAGTTTCAGCAGCAGAAGGGTGAGGAGATGCCAAATCTAGAAGGACATGACAGTGGTGATAATCAATCGGGTCCTGTTGAGGAGGTTCAGAAGGATGAATTTGAAACCGAAAATTTTGCAGAGGTTGTCTTGGTTCAACATGAACAAATCAAGCCAGAAACAGAAGTAGAGCATAATGAGAGAGATGTAGAATTCGAGCAACATAATGGTCCACGTAGGCAGTATCAAAACCAAAGAGGTGGCCGTGGAGGAGGCCGTAGAGGTTACTCTAATGGCCGTGGTGGCCGAGGCAGAGGAGGTGGTTCTTACCAGAATGGTCAGAACCAGTTTTATGACCAGCCTGGAAACTATTATTCAAGGAATTACAATAGGGCAAGGGGCGGTAGAGGTGGTGGTCCGTCTTACAACAACCATGGTTCAGCTCAAGATGGCCATGCCTCAGCTAACATTGGAGTTGCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1618

Protein Analysis

440

Amino Acids

48.11

Weight (kDa)

5.09

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 38
AatII GACGTC 1 cut(s) 357
Acc36I ACCTGC 1 cut(s) 411
AccB7I CCANNNNNTGG 1 cut(s) 651
AccBSI CCGCTC 1 cut(s) 336
AccII CGCG 1 cut(s) 69
AccIII TCCGGA 1 cut(s) 775
AcoI YGGCCR 4 cut(s) 1096, 1129, 1138, 1285
AcsI RAATTY 3 cut(s) 953, 967, 1046
AcyI GRCGYC 1 cut(s) 354
AdeI CACNNNGTG 1 cut(s) 569
AfaI GTAC 1 cut(s) 855
AfeI AGCGCT 1 cut(s) 629
AfiI CCNNNNNNNGG 5 cut(s) 106, 376, 379, 382, 651
AgsI TTSAA 3 cut(s) 959, 992, 1212
AjnI CCWGG 5 cut(s) 255, 375, 467, 584, 1195
AleI CACNNNNGTG 1 cut(s) 701
AluBI AGCT 8 cut(s) 196, 205, 394, 649, 773, 791, 1277, 1298
AluI AGCT 8 cut(s) 196, 205, 394, 649, 773, 791, 1277, 1298
Alw21I GWGCWC 2 cut(s) 198, 642
Alw26I GTCTC 2 cut(s) 31, 117
AlwNI CAGNNNCTG 1 cut(s) 794
Ama87I CYCGRG 2 cut(s) 149, 197
Aor13HI TCCGGA 1 cut(s) 775
Aor51HI AGCGCT 1 cut(s) 629
AoxI GGCC 8 cut(s) 34, 70, 587, 1096, 1108, 1129, 1138, 1285
ApeKI GCWGC 8 cut(s) 328, 489, 625, 630, 788, 791, 794, 865
ApoI RAATTY 3 cut(s) 953, 967, 1046
ArsI GACNNNNNNTTYG 2 cut(s) 833, 865
AspLEI GCGC 2 cut(s) 63, 630
AspS9I GGNCC 6 cut(s) 35, 40, 154, 926, 1064, 1250
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 6 cut(s) 253, 442, 457, 835, 887, 923
AvaI CYCGRG 2 cut(s) 149, 197
AvaII GGWCC 5 cut(s) 40, 154, 926, 1064, 1250
BalI TGGCCA 1 cut(s) 1287
BanII GRGCYC 2 cut(s) 198, 294
BauI CACGAG 1 cut(s) 476
Bbv12I GWGCWC 2 cut(s) 198, 642
BbvCI CCTCAGC 2 cut(s) 44, 1294
BbvI GCAGC 8 cut(s) 340, 476, 617, 637, 778, 781, 800, 877
BccI CCATC 5 cut(s) 97, 182, 194, 761, 1277
BceAI ACGGC 7 cut(s) 27, 49, 212, 236, 1083, 1095, 1116
BcgI CGANNNNNNTGC 2 cut(s) 953, 987
BciT130I CCWGG 5 cut(s) 257, 377, 469, 586, 1197
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 2 cut(s) 31, 117
BfaI CTAG 2 cut(s) 690, 893
BfmI CTRYAG 1 cut(s) 623
BfoI RGCGCY 1 cut(s) 631
BfuAI ACCTGC 1 cut(s) 411
BglI GCCNNNNNGGC 1 cut(s) 1137
Bme1390I CCNGG 6 cut(s) 38, 257, 377, 469, 586, 1197
Bme18I GGWCC 5 cut(s) 40, 154, 926, 1064, 1250
BmeT110I CYCGRG 2 cut(s) 149, 197
BmgT120I GGNCC 6 cut(s) 35, 40, 154, 926, 1064, 1250
BmiI GGNNCC 2 cut(s) 473, 927
BmrFI CCNGG 6 cut(s) 38, 257, 377, 469, 586, 1197
BmsI GCATC 3 cut(s) 72, 102, 873
BpmI CTGGAG 2 cut(s) 568, 744
Bpu10I CCTNAGC 2 cut(s) 44, 1294
BpuEI CTTGAG 1 cut(s) 1263
BpuMI CCSGG 1 cut(s) 38
BsaAI YACGTR 1 cut(s) 1070
BsaHI GRCGYC 1 cut(s) 354
BsaJI CCNNGG 8 cut(s) 9, 67, 121, 234, 1099, 1132, 1141, 1267
BsaWI WCCGGW 1 cut(s) 775
BsaXI ACNNNNNCTCC 4 cut(s) 253, 283, 742, 772
Bsc4I CCNNNNNNNGG 5 cut(s) 106, 376, 379, 382, 651
Bse118I RCCGGY 1 cut(s) 589
Bse1I ACTGG 1 cut(s) 1180
Bse3DI GCAATG 1 cut(s) 789
BseAI TCCGGA 1 cut(s) 775
BseBI CCWGG 5 cut(s) 257, 377, 469, 586, 1197
BseDI CCNNGG 8 cut(s) 9, 67, 121, 234, 1099, 1132, 1141, 1267
BseGI GGATG 3 cut(s) 63, 841, 955
BseLI CCNNNNNNNGG 5 cut(s) 106, 376, 379, 382, 651
BseMI GCAATG 1 cut(s) 789
BseMII CTCAG 5 cut(s) 58, 279, 580, 678, 1308
BseNI ACTGG 1 cut(s) 1180
BseRI GAGGAG 7 cut(s) 122, 404, 550, 893, 950, 1119, 1164
BseXI GCAGC 8 cut(s) 340, 476, 617, 637, 778, 781, 800, 877
BsgI GTGCAG 1 cut(s) 347
Bsh1236I CGCG 1 cut(s) 69
BshFI GGCC 8 cut(s) 36, 72, 589, 1098, 1110, 1131, 1140, 1287
BsiHKAI GWGCWC 2 cut(s) 198, 642
BsiHKCI CYCGRG 2 cut(s) 149, 197
BsiSI CCGG 3 cut(s) 38, 590, 776
BslI CCNNNNNNNGG 5 cut(s) 106, 376, 379, 382, 651
BsmAI GTCTC 2 cut(s) 31, 117
BsmI GAATGC 1 cut(s) 622
BsnI GGCC 8 cut(s) 36, 72, 589, 1098, 1110, 1131, 1140, 1287
BsoBI CYCGRG 2 cut(s) 149, 197
Bsp1286I GDGCHC 3 cut(s) 198, 294, 642
Bsp13I TCCGGA 1 cut(s) 775
Bsp1407I TGTACA 1 cut(s) 853
Bsp143I GATC 1 cut(s) 579
Bsp19I CCATGG 1 cut(s) 1267
BspANI GGCC 8 cut(s) 36, 72, 589, 1098, 1110, 1131, 1140, 1287
BspCNI CTCAG 5 cut(s) 57, 280, 579, 677, 1307
BspEI TCCGGA 1 cut(s) 775
BspFNI CGCG 1 cut(s) 69
BspLI GGNNCC 2 cut(s) 473, 927
BspMAI CTGCAG 1 cut(s) 627
BspMI ACCTGC 1 cut(s) 411
BsrBI CCGCTC 1 cut(s) 336
BsrDI GCAATG 1 cut(s) 789
BsrFI RCCGGY 1 cut(s) 589
BsrGI TGTACA 1 cut(s) 853
BsrI ACTGG 1 cut(s) 1180
BssAI RCCGGY 1 cut(s) 589
BssECI CCNNGG 8 cut(s) 9, 67, 121, 234, 1099, 1132, 1141, 1267
BssMI GATC 1 cut(s) 579
BssNI GRCGYC 1 cut(s) 354
BssSI CACGAG 1 cut(s) 476
BssT1I CCWWGG 2 cut(s) 121, 1267
Bst2BI CACGAG 1 cut(s) 476
Bst2UI CCWGG 5 cut(s) 257, 377, 469, 586, 1197
Bst4CI ACNGT 4 cut(s) 508, 845, 858, 908
BstACI GRCGYC 1 cut(s) 354
BstAPI GCANNNNNTGC 1 cut(s) 794
BstAUI TGTACA 1 cut(s) 853
BstBAI YACGTR 1 cut(s) 1070
BstC8I GCNNGC 4 cut(s) 212, 534, 642, 657
BstDEI CTNAG 6 cut(s) 44, 288, 566, 664, 722, 1294
BstDSI CCRYGG 5 cut(s) 9, 67, 1099, 1132, 1267
BstEII GGTNACC 1 cut(s) 259
BstF5I GGATG 3 cut(s) 63, 841, 955
BstFNI CGCG 1 cut(s) 69
BstH2I RGCGCY 1 cut(s) 631
BstHHI GCGC 2 cut(s) 63, 630
BstKTI GATC 1 cut(s) 582
BstMAI GTCTC 2 cut(s) 31, 117
BstMBI GATC 1 cut(s) 579
BstNI CCWGG 5 cut(s) 257, 377, 469, 586, 1197
BstPI GGTNACC 1 cut(s) 259
BstSCI CCNGG 6 cut(s) 36, 255, 375, 467, 584, 1195
BstSFI CTRYAG 1 cut(s) 623
BstUI CGCG 1 cut(s) 69
BstV1I GCAGC 8 cut(s) 340, 476, 617, 637, 778, 781, 800, 877
BstXI CCANNNNNNTGG 2 cut(s) 592, 1094
BsuRI GGCC 8 cut(s) 36, 72, 589, 1098, 1110, 1131, 1140, 1287
BtgI CCRYGG 5 cut(s) 9, 67, 1099, 1132, 1267
BtsCI GGATG 3 cut(s) 63, 841, 955
BtsIMutI CAGTG 4 cut(s) 510, 522, 574, 913
BveI ACCTGC 1 cut(s) 411
Cac8I GCNNGC 4 cut(s) 212, 534, 642, 657
CaiI CAGNNNCTG 1 cut(s) 794
CfoI GCGC 2 cut(s) 63, 630
Cfr10I RCCGGY 1 cut(s) 589
Cfr13I GGNCC 6 cut(s) 35, 40, 154, 926, 1064, 1250
Cfr42I CCGCGG 1 cut(s) 70
CseI GACGC 1 cut(s) 445
Csp6I GTAC 1 cut(s) 854
CviAII CATG 4 cut(s) 902, 995, 1268, 1289
CviQI GTAC 1 cut(s) 854
DdeI CTNAG 6 cut(s) 44, 288, 566, 664, 722, 1294
DpnI GATC 1 cut(s) 581
DpnII GATC 1 cut(s) 579
DraIII CACNNNGTG 1 cut(s) 569
DrdI GACNNNNNNGTC 1 cut(s) 38
DseDI GACNNNNNNGTC 1 cut(s) 38
EaeI YGGCCR 4 cut(s) 1096, 1129, 1138, 1285
EciI GGCGGA 2 cut(s) 160, 334
Ecl136II GAGCTC 1 cut(s) 196
Eco130I CCWWGG 2 cut(s) 121, 1267
Eco24I GRGCYC 2 cut(s) 198, 294
Eco47I GGWCC 5 cut(s) 40, 154, 926, 1064, 1250
Eco47III AGCGCT 1 cut(s) 629
Eco53kI GAGCTC 1 cut(s) 196
Eco88I CYCGRG 2 cut(s) 149, 197
Eco91I GGTNACC 1 cut(s) 259
EcoICRI GAGCTC 1 cut(s) 196
EcoO109I RGGNCCY 2 cut(s) 154, 926
EcoO65I GGTNACC 1 cut(s) 259
EcoRI GAATTC 1 cut(s) 1046
EcoRII CCWGG 5 cut(s) 255, 375, 467, 584, 1195
EcoT14I CCWWGG 2 cut(s) 121, 1267
EcoT38I GRGCYC 2 cut(s) 198, 294
ErhI CCWWGG 2 cut(s) 121, 1267
FaeI CATG 4 cut(s) 905, 998, 1271, 1292
FatI CATG 4 cut(s) 901, 994, 1267, 1288
FauI CCCGC 1 cut(s) 280
FauNDI CATATG 1 cut(s) 700
FokI GGATG 3 cut(s) 50, 848, 962
FriOI GRGCYC 2 cut(s) 198, 294
FspBI CTAG 2 cut(s) 690, 893
GlaI GCGC 2 cut(s) 62, 629
GsuI CTGGAG 2 cut(s) 568, 744
HaeII RGCGCY 1 cut(s) 631
HaeIII GGCC 8 cut(s) 36, 72, 589, 1098, 1110, 1131, 1140, 1287
HapII CCGG 3 cut(s) 38, 590, 776
HgaI GACGC 1 cut(s) 445
HhaI GCGC 2 cut(s) 63, 630
Hin1I GRCGYC 1 cut(s) 354
Hin1II CATG 4 cut(s) 905, 998, 1271, 1292
Hin6I GCGC 2 cut(s) 61, 628
HinP1I GCGC 2 cut(s) 61, 628
HindIII AAGCTT 1 cut(s) 203
HinfI GANTC 2 cut(s) 110, 341
HpaII CCGG 3 cut(s) 38, 590, 776
HphI GGTGA 6 cut(s) 253, 442, 457, 835, 887, 923
Hpy166II GTNNAC 3 cut(s) 465, 841, 1067
Hpy188I TCNGA 7 cut(s) 22, 31, 399, 667, 742, 945, 1176
Hpy188III TCNNGA 6 cut(s) 199, 433, 761, 776, 893, 1280
Hpy8I GTNNAC 3 cut(s) 465, 841, 1067
Hpy99I CGWCG 6 cut(s) 35, 194, 227, 233, 356, 359
HpyAV CCTTC 4 cut(s) 535, 865, 890, 940
HpyCH4III ACNGT 4 cut(s) 508, 845, 858, 908
HpyCH4IV ACGT 2 cut(s) 354, 1069
HpyCH4V TGCA 4 cut(s) 328, 625, 633, 974
HpyF3I CTNAG 6 cut(s) 44, 288, 566, 664, 722, 1294
HpySE526I ACGT 2 cut(s) 354, 1069
Hsp92I GRCGYC 1 cut(s) 354
Hsp92II CATG 4 cut(s) 905, 998, 1271, 1292
HspAI GCGC 2 cut(s) 61, 628
Kpn2I TCCGGA 1 cut(s) 775
KspI CCGCGG 1 cut(s) 70
Kzo9I GATC 1 cut(s) 579
LmnI GCTCC 4 cut(s) 391, 471, 778, 817
Lsp1109I GCAGC 8 cut(s) 340, 476, 617, 637, 778, 781, 800, 877
LweI GCATC 3 cut(s) 72, 102, 873
MaeI CTAG 2 cut(s) 690, 893
MaeII ACGT 2 cut(s) 354, 1069
MaeIII GTNAC 5 cut(s) 259, 443, 508, 694, 1118
MalI GATC 1 cut(s) 581
MbiI CCGCTC 1 cut(s) 336
MboI GATC 1 cut(s) 579
MboII GAAGA 1 cut(s) 308
MfeI CAATTG 1 cut(s) 675
MhlI GDGCHC 3 cut(s) 198, 294, 642
MlsI TGGCCA 1 cut(s) 1287
MluCI AATT 6 cut(s) 675, 685, 953, 967, 1046, 1216
MluNI TGGCCA 1 cut(s) 1287
MlyI GAGTC 1 cut(s) 119
MmeI TCCRAC 1 cut(s) 54
Mox20I TGGCCA 1 cut(s) 1287
MroI TCCGGA 1 cut(s) 775
MscI TGGCCA 1 cut(s) 1287
MseI TTAA 1 cut(s) 1318
MslI CAYNNNNRTG 4 cut(s) 527, 615, 701, 906
Msp20I TGGCCA 1 cut(s) 1287
MspA1I CMGCKG 2 cut(s) 69, 791
MspI CCGG 3 cut(s) 38, 590, 776
MspR9I CCNGG 6 cut(s) 38, 257, 377, 469, 586, 1197
MunI CAATTG 1 cut(s) 675
Mva1269I GAATGC 1 cut(s) 622
MvaI CCWGG 5 cut(s) 257, 377, 469, 586, 1197
MvnI CGCG 1 cut(s) 69
NciI CCSGG 1 cut(s) 38
NcoI CCATGG 1 cut(s) 1267
NdeI CATATG 1 cut(s) 700
NdeII GATC 1 cut(s) 579
NlaIII CATG 4 cut(s) 905, 998, 1271, 1292
NlaIV GGNNCC 2 cut(s) 473, 927
NmeAIII GCCGAG 3 cut(s) 259, 562, 1166
NmuCI GTSAC 4 cut(s) 259, 443, 508, 694
OliI CACNNNNGTG 1 cut(s) 701
PaeR7I CTCGAG 2 cut(s) 149, 197
PcsI WCGNNNNNNNCGW 3 cut(s) 147, 195, 231
PctI GAATGC 1 cut(s) 622
PfeI GAWTC 1 cut(s) 341
PflFI GACNNNGTC 1 cut(s) 506
PflMI CCANNNNNTGG 1 cut(s) 651
PleI GAGTC 1 cut(s) 118
PpsI GAGTC 1 cut(s) 118
Ppu21I YACGTR 1 cut(s) 1070
PpuMI RGGWCCY 2 cut(s) 154, 926
Psp124BI GAGCTC 1 cut(s) 198
Psp5II RGGWCCY 2 cut(s) 154, 926
Psp6I CCWGG 5 cut(s) 255, 375, 467, 584, 1195
PspEI GGTNACC 1 cut(s) 259
PspGI CCWGG 5 cut(s) 255, 375, 467, 584, 1195
PspN4I GGNNCC 2 cut(s) 473, 927
PspPI GGNCC 6 cut(s) 35, 40, 154, 926, 1064, 1250
PspPPI RGGWCCY 2 cut(s) 154, 926
PspXI VCTCGAGB 1 cut(s) 149
PsrI GAACNNNNNNTAC 2 cut(s) 392, 424
PstI CTGCAG 1 cut(s) 627
PstNI CAGNNNCTG 1 cut(s) 794
PsyI GACNNNGTC 1 cut(s) 506
PvuII CAGCTG 1 cut(s) 791
RsaI GTAC 1 cut(s) 855
RsaNI GTAC 1 cut(s) 854
RseI CAYNNNNRTG 4 cut(s) 527, 615, 701, 906
SacI GAGCTC 1 cut(s) 198
SacII CCGCGG 1 cut(s) 70
SaqAI TTAA 1 cut(s) 1318
Sau3AI GATC 1 cut(s) 579
Sau96I GGNCC 6 cut(s) 35, 40, 154, 926, 1064, 1250
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 6 cut(s) 38, 257, 377, 469, 586, 1197
SduI GDGCHC 3 cut(s) 198, 294, 642
SfaNI GCATC 3 cut(s) 72, 102, 873
SfcI CTRYAG 1 cut(s) 623
SfiI GGCCNNNNNGGCC 1 cut(s) 1137
Sfr274I CTCGAG 2 cut(s) 149, 197
Sfr303I CCGCGG 1 cut(s) 70
SgrBI CCGCGG 1 cut(s) 70
SinI GGWCC 5 cut(s) 40, 154, 926, 1064, 1250
SlaI CTCGAG 2 cut(s) 149, 197
SmiMI CAYNNNNRTG 4 cut(s) 527, 615, 701, 906
SmlI CTYRAG 3 cut(s) 149, 197, 1278
SmoI CTYRAG 3 cut(s) 149, 197, 1278
Sse9I AATT 6 cut(s) 675, 685, 953, 967, 1046, 1216
SspMI CTAG 2 cut(s) 690, 893
SstI GAGCTC 1 cut(s) 198
StyD4I CCNGG 6 cut(s) 36, 255, 375, 467, 584, 1195
StyI CCWWGG 2 cut(s) 121, 1267
TaaI ACNGT 4 cut(s) 508, 845, 858, 908
TaiI ACGT 2 cut(s) 357, 1072
TaqI TCGA 7 cut(s) 150, 189, 198, 357, 426, 455, 1050
TasI AATT 6 cut(s) 675, 685, 953, 967, 1046, 1216
TatI WGTACW 1 cut(s) 853
TauI GCSGC 7 cut(s) 72, 278, 281, 284, 339, 350, 383
TfiI GAWTC 1 cut(s) 341
Tru1I TTAA 1 cut(s) 1318
Tru9I TTAA 1 cut(s) 1318
TscAI CASTG 4 cut(s) 517, 529, 574, 913
TseFI GTSAC 4 cut(s) 259, 443, 508, 694
TseI GCWGC 8 cut(s) 328, 489, 625, 630, 788, 791, 794, 865
Tsp45I GTSAC 4 cut(s) 259, 443, 508, 694
TspDTI ATGAA 4 cut(s) 782, 837, 966, 1011
TspGWI ACGGA 3 cut(s) 211, 804, 1242
TspRI CASTG 4 cut(s) 517, 529, 574, 913
Tth111I GACNNNGTC 1 cut(s) 506
Van91I CCANNNNNTGG 1 cut(s) 651
VpaK11BI GGWCC 5 cut(s) 40, 154, 926, 1064, 1250
XapI RAATTY 3 cut(s) 953, 967, 1046
XbaI TCTAGA 1 cut(s) 892
XhoI CTCGAG 2 cut(s) 149, 197
XspI CTAG 2 cut(s) 690, 893
ZraI GACGTC 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.