FvH4_4g36790

Serine threonine-protein kinase sepA-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006663
Physical Location & Seq
Forward (+)
33554731 .. 33565905
11175 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36790.t7

Sequence Viewer

Length: 4260 bp
ATGTCTCGGCAGGCTTCGTCGCCTCACTTCCACAAATCCAAGACTCTCGATAACAAATACATGCTTGGAGATGAGATTGGAAAGGGAGCATATGGTCGAGTTTACAAGGGTTTGGATTTGGAGAATGGAGACTTTGTTGCTATTAAACAGGTTTCTTTGGAGAACATTGCGCAGGAGGATCTCAACGTTATAATGCAAGAGATTGATTTACTGAAGAATTTAAATCACAAAAACATTGTGAAATATCTTGGATCCTTAAAGACAAAGACACATCTTCACATAGTCCTAGAATACGTGGAGAACGGCTCACTTGCAAACATCATTAAGCCAAATAAGTTTGGACCTTTTCCAGAATCACTGGTAGCTGTGTACATTGCTCAGGTTTTGGAAGGGTTGGTGTATTTACATGAACAGGGTGTTATCCATCGAGATATCAAGGGTGCAAATATTTTGACAACTAAAGAGGGCCTTGTGAAACTTGCTGATTTTGGTGTTGCTACAAAATTGACCGAGGCTGATGTGAATACACATTCAGTTGTTGGAACGCCATATTGGATGGCCCCAGAGGTGATCGAAATGTCAGGGGTTTGTGCTGCTTCAGATATTTGGAGCGTTGGCTGCACCGTTATTGAACTTCTTACATGTGTACCTCCTTACTATGATTTACAGCCGATGCCAGCTCTATTTCGTATTGTTCAGGATGAACATCCTCCCATTCCTGACAGTCTATCTCCTGACATTACTGATTTTCTGTGTCAATGCTTTAAAAAGGATGCTAGGCACCGGCCTGATGCAAAAACACTGTTATCTCATCCTTGGATACAAAACTGCAGGCGAGCGTTGCAATCATCAATTCGTCATAGTGGAACATTAAGAGATGTACAGGAAGATGTTTCTATGGGTGCAGAACTCTCCAATGGAGATATTCGGAGCTCTGGCGAAAGTCCTGCAGAGAAAACGGAAGAAGCAACTTCTGCTATTAAAGCAGATTCTAGGAAAGAGTTATTATCAACCGGCATTTCTGATGTGAGAAAATCTGGGGAGGATCCTGCTTCAGATGTGAAATCTGTTGAGGAAAAAGCAGATGGTTTAGAAAATGATCTGACAGATCCAGCTCCCACCTTAGCTATACATGACAAATCATCTTTGCAAAATGGTTCTGGCAGAATATCTTCTAATAAGGAATTGGCTGCCTCTGAGGCAGCTGAGCTAGATGATCGAGCACATACGGCCAACATTGATGAACCACCAATGAATGGTGAGGTTAAATCTCCTGAATTGACGACAAAAAGTGTAACAAAACATGGCAAAGGAAACTCTATTGGGTTTAGATCATTTGGTTTCGGAGCAAGAAATCAGGATGGTACTTTTGAAAAGGCCTCGAAGATGCCAGTAAGTATGGGAGGGAATGAACTCAGTAAATTTAGTGACACTCCAGGAGACGCTTCCTTGGAAGATCTATTTCATCCTTTGGATAAGCATCCCGATGATAGGGCATTTGAAGCGTCAACATCTTCTTCTGTATCACATGTGAACCAAGGAAATACATCTGTAAATGACGCTGGAAAAAGTGACCTGGCTACGAAGTTAAGAGCTACAATTGCGCAAAAGCAAATGGAGAGTGAAATGGGTCAGGCTAATGGCAGTGGTGGTAACTTGTTACAGCTGATGATGGGTGTTCTCCAAGATGATGTGATTGACATAGGTGGTTTGGTATTTGATGAAAAGTTGCCCGGAGAAAACCTTTTTCCCCTACAGGCTGTTGAGTTTAGCCGGTTAGTTGGATCACTGAAACTAGATGAATCAGAAGATGTTGTTGTCTCTGCCTGCCAGAAACTTATTGCTATATTTCATCAGCGCCCGGAGCAGAAAATTGTTTTTGTCACGCAGCATGGTTTGCTCCCTTTAATGGAATTGCTTGAAGTTCCTAAAACTCGAGTTATATGTTCTGTGCTTCAAATCATAAACCAGATAATCAAGGATAATACTGATTTCCAGGAAAATGCTTGTCATGTTGGTCTGATCCCAGTGGTAATGAGCTTTGCGGTACCTAGTCATTCTCGAGAAATTCGTATGGAAGCAGCTTATTTCTTGCAACAGCTTTGTCAGTCAAGCCCGTTGACTCTGCAAATGTTCATAGCTTGCCGTGGAATACCTGTTCTGGTGGGCTTTTTAGAGGCTGATTATGCAAAATTCAGGGAAATGGTTCATCTAGCTATTGATGGCATGTGGCAAGTCTTTAAGCTTCAGCGATCAACTCCTAGGAATGATTTCTGTCGCATAGCTGCGAAAAATGGAATTCTCCTGAGGCTTATTAACACCCTTTATAGCCTGAATGAGGCAACCCGTTTAGCTTCCATTTCTGGTGGGACTGGAATTCCTCTAGATGGTTCTGCTCAGCGCCCACGGTCTGGTTCATTGGATCCTGGTCATCCCACTTTTGCTCAGAGTGATGGACCACTTTCTGATCATAATGATCACTCGAAAATTAGGCATGGCATAAATGATTCACATTTGTCTACTGGAGCAGTGGAACCGGCACGTGCATCAACTTCTAATTCTCAAAGATCAGATGCCAATCAATCAGATCCCCGGTATCTTCATCTAGATACTGATAGACCTCAATCTAGCAATGGAGTGGGAGATACTTCGGTTTCTTCTAAATTGCAAGAGTCAACAGGTGCAGATAAAGTAATAAATATGTCATCAAAGGAAACTTCAACTACTTCTCGAGGAGATCTTGACCTTAGGCAGCAGCGTGCTCCTATATCTCTTCATAGATCAGCCACTGATAGACATCCCAAAATGATGGAAGGTACATCAAATGGCTTATCAACAACAGCTGCTTCTCAACAGGAGCAAGTTCGGCCTCTTCTTAGCTTGTTGGATAAAGAACCCCCTTCGAGACATTTTTCTGGTCAGCTTGAGTATGTACGCCACCTCCCTGGGTTGGAGAGACATGAAAGTATACTGCCCCTTTTACATGCCTCTAACGAAAAGAAAACAAATGGTGAATTAGATTTCCTTATGGCAGAGTTTGCAGACGTCTCTCAACGTGGTAGAGAGAAAGGGAATCTTGATTCTACTACTAGAGTTCCCCCCAAAACAATAAATAAGGAGATGGGAATACTAGCATCTAACAAAGGAGCTGCTTCCACCTCCGCATCTCAGACAGCATCAGGGGTATTGTCTGGTTCGGGTGTTCTGAATGCTAGACCAGGCAGTGCAACATCATCGGGTTTACTCTCGCACATGGTATCAACATTGAATGCAGACGTTGCAAGGGAATACCTAGAAAAGGTGGCAGATCTTCTACTTGAGTTTGCACGAGCAGATACTACTGTAAAATCATACATGTGTAGCCAAAGTTTGCTCAGCCGTCTTTTTCAGATGTTTAATAGGGTGGAGCCCCCAATTCTTTTAAAGATACTGAAGTGTGTCAACCATTTGTCAACTGATCCAAACTGCTTAGAGAATCTTCAGCGGGCAGATGCAATCAAATATTTGATTCCAAATCTTGAACTCAAAGAAGGATCTCTTGTATCTCAAATACATCATGAGGTCCTAAATGCCTTGTTCAATCTCTGTAAGATTAACAAGAGGAGACAGGAACAAGCAGCTGAGAATGGAATAATTCCACACTTGATGCACTTTATCGAGTCTAATTCTCCTCTGAAACAATATGCGTTGCCTTTGCTCTGTGATATGGCTCATGCTTCACGTAACTCAAGAGAGCAATTAAGGGCCCATGGTGGGTTGGATGTGTACTTGAGCCTTCTAGAGGATGAGCTTTGGTCTGTGACTGCATTGGATTCAATTGCTGTTTGTTTAGCTCATGACAATGACAACCGGAAGGTAGAACAAGCACTGCTGAAAAAGGATGCAGTTCAGAAGTTGGTGAAATTCTTCCAGTGCTGTCCAGAACAGTATTTTGTGCACATATTGGAGCCATTTTTGAAAATTATCACGAAATCGTCTCGAATTAATACAACATTAGCTGTTAATGGTTTGACACCGCTGCTTATCACAAGACTTGACCATCAGGATGCTATTGCCCGTTTAAATCTTCTTAAGCTGATAAAGGCTGTTTATGAGCACCACCCACGGCCAAAGCAATTGATTGTGGAAAATGATCTGCCTCAAAAGCTTCAGAATCTAATAGAGGAACGCAGAGATGGGCAACGCTCTGGAGGCCAAGTATTAGTGAAACAAATGGCTACCTCCTTGCTAAAAGCACTCCATATAAACACTGTATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000278 GO:0000281 GO:0000910 GO:0001558 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005813 GO:0005815 GO:0005829 GO:0005856 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007049 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0008361 GO:0009893 GO:0009987 GO:0010324 GO:0010562 GO:0010604 GO:0015630 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022402 GO:0022603 GO:0022604 GO:0023014 GO:0023052 GO:0030587 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032147 GO:0032268 GO:0032270 GO:0032502 GO:0032506 GO:0032535 GO:0033554 GO:0033674 GO:0035556 GO:0036089 GO:0036211 GO:0040008 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0045995 GO:0046777 GO:0048518 GO:0048522 GO:0048638 GO:0048856 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051246 GO:0051247 GO:0051301 GO:0051302 GO:0051338 GO:0051347 GO:0051510 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0061024 GO:0061387 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090066 GO:0090702 GO:0099024 GO:0099120 GO:0140096 GO:1901564 GO:1903047 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1420

Amino Acids

155.91

Weight (kDa)

6.12

Isoelectric Point (pI)

42.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 191
AatII GACGTC 1 cut(s) 3048
Acc16I TGCGCA 2 cut(s) 171, 1605
Acc65I GGTACC 1 cut(s) 2047
AccB1I GGYRCC 2 cut(s) 780, 2047
AccB7I CCANNNNNTGG 2 cut(s) 1256, 2411
AccI GTMKAC 2 cut(s) 2519, 2968
AciI CCGC 4 cut(s) 2045, 3162, 3484, 4016
AclI AACGTT 1 cut(s) 186
AcoI YGGCCR 2 cut(s) 1230, 4106
AcsI RAATTY 7 cut(s) 217, 1421, 2067, 2192, 2298, 2376, 3902
AcuI CTGAAG 7 cut(s) 233, 582, 1038, 2231, 3452, 3464, 4133
AcvI CACGTG 1 cut(s) 2543
AcyI GRCGYC 1 cut(s) 3045
AfaI GTAC 8 cut(s) 371, 648, 882, 1366, 2049, 2818, 2934, 3767
AflII CTTAAG 1 cut(s) 4070
AflIII ACRYGT 3 cut(s) 641, 1528, 3354
AjnI CCWGG 6 cut(s) 1435, 1575, 1995, 2425, 2944, 3217
AjuI GAANNNNNNNTTGG 2 cut(s) 535, 567
AloI GAACNNNNNNTCC 2 cut(s) 2142, 2174
Alw21I GWGCWC 5 cut(s) 935, 1225, 2764, 3939, 4098
Alw26I GTCTC 9 cut(s) 9, 123, 1434, 1825, 2899, 2950, 3052, 3598, 3981
Alw44I GTGCAC 1 cut(s) 3935
AlwNI CAGNNNCTG 1 cut(s) 2789
Ama87I CYCGRG 3 cut(s) 1935, 2061, 2730
AoxI GGCC 9 cut(s) 466, 558, 785, 1230, 1377, 2867, 3744, 4106, 4192
ApaI GGGCCC 1 cut(s) 3748
ApaLI GTGCAC 1 cut(s) 3935
ApoI RAATTY 7 cut(s) 217, 1421, 2067, 2192, 2298, 2376, 3902
AseI ATTAAT 1 cut(s) 3984
Asp700I GAANNNNTTC 4 cut(s) 1171, 2205, 2270, 3905
Asp718I GGTACC 1 cut(s) 2047
AspA2I CCTAGG 1 cut(s) 2261
AspLEI GCGC 4 cut(s) 172, 1606, 1860, 2403
AspS9I GGNCC 7 cut(s) 341, 466, 559, 2456, 3562, 3744, 3745
AsuC2I CCSGG 3 cut(s) 1734, 1862, 2593
AsuHPI GGTGA 4 cut(s) 580, 1271, 3023, 3910
AvaI CYCGRG 3 cut(s) 1935, 2061, 2730
AvaII GGWCC 3 cut(s) 341, 2456, 3562
AvrII CCTAGG 1 cut(s) 2261
AxyI CCTNAGG 2 cut(s) 2306, 2747
BaeGI GKGCMC 2 cut(s) 3748, 3939
BaeI ACNNNNGTAYC 8 cut(s) 630, 663, 2637, 2670, 3327, 3360, 3419, 3452
BamHI GGATCC 3 cut(s) 251, 1045, 2422
BanI GGYRCC 2 cut(s) 780, 2047
BanII GRGCYC 3 cut(s) 935, 3411, 3748
BauI CACGAG 1 cut(s) 3327
BbrPI CACGTG 1 cut(s) 2543
Bbv12I GWGCWC 5 cut(s) 935, 1225, 2764, 3939, 4098
BceAI ACGGC 5 cut(s) 319, 1245, 2130, 3363, 4121
BcgI CGANNNNNNTGC 4 cut(s) 929, 963, 3216, 3250
BciT130I CCWGG 6 cut(s) 1437, 1577, 1997, 2427, 2946, 3219
BciVI GTATCC 1 cut(s) 813
BclI TGATCA 2 cut(s) 2467, 2476
BcnI CCSGG 3 cut(s) 1734, 1862, 2593
BcoDI GTCTC 9 cut(s) 9, 123, 1434, 1825, 2899, 2950, 3052, 3598, 3981
BfmI CTRYAG 3 cut(s) 829, 948, 1754
BfoI RGCGCY 2 cut(s) 1861, 2404
BfrI CTTAAG 1 cut(s) 4070
BfuI GTATCC 1 cut(s) 813
BglI GCCNNNNNGGC 1 cut(s) 1199
BglII AGATCT 3 cut(s) 1456, 2737, 3307
BlnI CCTAGG 1 cut(s) 2261
BlpI GCTNAGC 3 cut(s) 1206, 2397, 3374
Bme1390I CCNGG 9 cut(s) 1437, 1577, 1734, 1862, 1997, 2427, 2593, 2946, 3219
Bme18I GGWCC 3 cut(s) 341, 2456, 3562
BmeT110I CYCGRG 3 cut(s) 1935, 2061, 2730
BmgT120I GGNCC 7 cut(s) 341, 466, 559, 2456, 3562, 3744, 3745
BmrFI CCNGG 9 cut(s) 1437, 1577, 1734, 1862, 1997, 2427, 2593, 2946, 3219
BmrI ACTGGG 1 cut(s) 2021
BmuI ACTGGG 1 cut(s) 2021
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 3 cut(s) 1419, 2544, 4209
Bpu10I CCTNAGC 2 cut(s) 378, 1123
Bpu1102I GCTNAGC 3 cut(s) 1206, 2397, 3374
BpuEI CTTGAG 4 cut(s) 2945, 3338, 3712, 3790
BpuMI CCSGG 3 cut(s) 1734, 1862, 2593
BsaAI YACGTR 3 cut(s) 295, 2543, 3722
BsaBI GATNNNNATC 4 cut(s) 705, 1479, 2577, 2796
BsaHI GRCGYC 1 cut(s) 3045
BsaWI WCCGGW 1 cut(s) 3849
BsaXI ACNNNNNCTCC 6 cut(s) 120, 150, 2925, 2955, 4182, 4212
Bse118I RCCGGY 4 cut(s) 783, 1013, 1773, 2536
Bse1I ACTGG 6 cut(s) 363, 1391, 2027, 2377, 2527, 3910
Bse21I CCTNAGG 2 cut(s) 2306, 2747
Bse3DI GCAATG 3 cut(s) 165, 372, 2638
Bse8I GATNNNNATC 4 cut(s) 705, 1479, 2577, 2796
BseBI CCWGG 6 cut(s) 1437, 1577, 1997, 2427, 2946, 3219
BseJI GATNNNNATC 4 cut(s) 705, 1479, 2577, 2796
BseMI GCAATG 3 cut(s) 165, 372, 2638
BseNI ACTGG 6 cut(s) 363, 1391, 2027, 2377, 2527, 3910
BseRI GAGGAG 3 cut(s) 2748, 3616, 3660
BseSI GKGCMC 2 cut(s) 3748, 3939
BsgI GTGCAG 3 cut(s) 604, 924, 2703
BshFI GGCC 9 cut(s) 468, 560, 787, 1232, 1379, 2869, 3746, 4108, 4194
BshNI GGYRCC 2 cut(s) 780, 2047
BsiHKAI GWGCWC 5 cut(s) 935, 1225, 2764, 3939, 4098
BsiHKCI CYCGRG 3 cut(s) 1935, 2061, 2730
BsiSI CCGG 8 cut(s) 784, 1014, 1734, 1774, 1862, 2537, 2593, 3850
BslFI GGGAC 1 cut(s) 2383
BsmAI GTCTC 9 cut(s) 9, 123, 1434, 1825, 2899, 2950, 3052, 3598, 3981
BsmBI CGTCTC 3 cut(s) 1434, 3052, 3981
BsmFI GGGAC 1 cut(s) 2383
BsmI GAATGC 2 cut(s) 3214, 3274
BsnI GGCC 9 cut(s) 468, 560, 787, 1232, 1379, 2869, 3746, 4108, 4194
BsoBI CYCGRG 3 cut(s) 1935, 2061, 2730
Bsp120I GGGCCC 1 cut(s) 3744
Bsp1286I GDGCHC 7 cut(s) 935, 1225, 2764, 3411, 3748, 3939, 4098
Bsp1407I TGTACA 2 cut(s) 369, 880
Bsp1720I GCTNAGC 3 cut(s) 1206, 2397, 3374
Bsp19I CCATGG 1 cut(s) 3748
BspACI CCGC 4 cut(s) 2045, 3162, 3484, 4016
BspANI GGCC 9 cut(s) 468, 560, 787, 1232, 1379, 2869, 3746, 4108, 4194
BspHI TCATGA 2 cut(s) 3556, 3835
BspMAI CTGCAG 2 cut(s) 833, 952
BspT107I GGYRCC 2 cut(s) 780, 2047
BspTI CTTAAG 1 cut(s) 4070
BsrDI GCAATG 3 cut(s) 165, 372, 2638
BsrFI RCCGGY 4 cut(s) 783, 1013, 1773, 2536
BsrGI TGTACA 2 cut(s) 369, 880
BsrI ACTGG 6 cut(s) 363, 1391, 2027, 2377, 2527, 3910
BssAI RCCGGY 4 cut(s) 783, 1013, 1773, 2536
BssNAI GTATAC 1 cut(s) 2969
BssNI GRCGYC 1 cut(s) 3045
BssSI CACGAG 1 cut(s) 3327
BssT1I CCWWGG 5 cut(s) 815, 1449, 1537, 2261, 3748
Bst1107I GTATAC 1 cut(s) 2969
Bst2BI CACGAG 1 cut(s) 3327
Bst2UI CCWGG 6 cut(s) 1437, 1577, 1997, 2427, 2946, 3219
Bst4CI ACNGT 7 cut(s) 625, 725, 804, 2409, 3343, 3927, 4252
Bst6I CTCTTC 2 cut(s) 2778, 2877
BstACI GRCGYC 1 cut(s) 3045
BstAFI CTTAAG 1 cut(s) 4070
BstAPI GCANNNNNTGC 4 cut(s) 974, 1897, 3038, 3278
BstAUI TGTACA 2 cut(s) 369, 880
BstBAI YACGTR 3 cut(s) 295, 2543, 3722
BstC8I GCNNGC 8 cut(s) 12, 678, 833, 837, 1828, 2143, 2760, 3486
BstDSI CCRYGG 4 cut(s) 2146, 2405, 3748, 4103
BstENI CCTNNNNNAGG 3 cut(s) 2336, 3296, 3779
BstH2I RGCGCY 2 cut(s) 1861, 2404
BstHHI GCGC 4 cut(s) 172, 1606, 1860, 2403
BstMAI GTCTC 9 cut(s) 9, 123, 1434, 1825, 2899, 2950, 3052, 3598, 3981
BstNI CCWGG 6 cut(s) 1437, 1577, 1997, 2427, 2946, 3219
BstNSI RCATGY 6 cut(s) 64, 645, 1532, 2230, 2987, 3358
BstSCI CCNGG 9 cut(s) 1435, 1575, 1732, 1860, 1995, 2425, 2591, 2944, 3217
BstSFI CTRYAG 3 cut(s) 829, 948, 1754
BstSLI GKGCMC 2 cut(s) 3748, 3939
BstXI CCANNNNNNTGG 2 cut(s) 2809, 2945
BstZ17I GTATAC 1 cut(s) 2969
Bsu36I CCTNAGG 2 cut(s) 2306, 2747
BsuI GTATCC 1 cut(s) 813
BsuRI GGCC 9 cut(s) 468, 560, 787, 1232, 1379, 2869, 3746, 4108, 4194
BtgI CCRYGG 4 cut(s) 2146, 2405, 3748, 4103
BtsI GCAGTG 4 cut(s) 1651, 2535, 3229, 3866
Cac8I GCNNGC 8 cut(s) 12, 678, 833, 837, 1828, 2143, 2760, 3486
CaiI CAGNNNCTG 1 cut(s) 2789
CciI TCATGA 2 cut(s) 3556, 3835
CfoI GCGC 4 cut(s) 172, 1606, 1860, 2403
Cfr10I RCCGGY 4 cut(s) 783, 1013, 1773, 2536
Cfr13I GGNCC 7 cut(s) 341, 466, 559, 2456, 3562, 3744, 3745
CseI GACGC 3 cut(s) 1451, 1494, 1568
Csp6I GTAC 8 cut(s) 370, 647, 881, 1365, 2048, 2817, 2933, 3766
CviQI GTAC 8 cut(s) 370, 647, 881, 1365, 2048, 2817, 2933, 3766
DraI TTTAAA 4 cut(s) 222, 766, 3423, 4062
EaeI YGGCCR 2 cut(s) 1230, 4106
Eam1104I CTCTTC 2 cut(s) 2778, 2877
EarI CTCTTC 2 cut(s) 2778, 2877
Ecl136II GAGCTC 1 cut(s) 933
Eco130I CCWWGG 5 cut(s) 815, 1449, 1537, 2261, 3748
Eco147I AGGCCT 1 cut(s) 1379
Eco24I GRGCYC 3 cut(s) 935, 3411, 3748
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 3 cut(s) 341, 2456, 3562
Eco53kI GAGCTC 1 cut(s) 933
Eco57I CTGAAG 7 cut(s) 233, 582, 1038, 2231, 3452, 3464, 4133
Eco72I CACGTG 1 cut(s) 2543
Eco81I CCTNAGG 2 cut(s) 2306, 2747
Eco88I CYCGRG 3 cut(s) 1935, 2061, 2730
EcoICRI GAGCTC 1 cut(s) 933
EcoNI CCTNNNNNAGG 3 cut(s) 2336, 3296, 3779
EcoO109I RGGNCCY 3 cut(s) 466, 3562, 3744
EcoRI GAATTC 2 cut(s) 2298, 2376
EcoRII CCWGG 6 cut(s) 1435, 1575, 1995, 2425, 2944, 3217
EcoRV GATATC 1 cut(s) 433
EcoT14I CCWWGG 5 cut(s) 815, 1449, 1537, 2261, 3748
EcoT38I GRGCYC 3 cut(s) 935, 3411, 3748
ErhI CCWWGG 5 cut(s) 815, 1449, 1537, 2261, 3748
Esp3I CGTCTC 3 cut(s) 1434, 3052, 3981
FalI AAGNNNNNCTT 6 cut(s) 453, 485, 3060, 3092, 3522, 3554
FaqI GGGAC 1 cut(s) 2383
FauI CCCGC 1 cut(s) 3477
FauNDI CATATG 1 cut(s) 91
FbaI TGATCA 2 cut(s) 2467, 2476
FblI GTMKAC 2 cut(s) 2519, 2968
FriOI GRGCYC 3 cut(s) 935, 3411, 3748
FspI TGCGCA 2 cut(s) 171, 1605
GlaI GCGC 4 cut(s) 171, 1605, 1859, 2402
GsuI CTGGAG 3 cut(s) 1419, 2544, 4209
HaeII RGCGCY 2 cut(s) 1861, 2404
HaeIII GGCC 9 cut(s) 468, 560, 787, 1232, 1379, 2869, 3746, 4108, 4194
HapII CCGG 8 cut(s) 784, 1014, 1734, 1774, 1862, 2537, 2593, 3850
HgaI GACGC 3 cut(s) 1451, 1494, 1568
HhaI GCGC 4 cut(s) 172, 1606, 1860, 2403
Hin1I GRCGYC 1 cut(s) 3045
Hin6I GCGC 4 cut(s) 170, 1604, 1858, 2401
HinP1I GCGC 4 cut(s) 170, 1604, 1858, 2401
HincII GTYRAC 5 cut(s) 1509, 2121, 2676, 3442, 3453
HindII GTYRAC 5 cut(s) 1509, 2121, 2676, 3442, 3453
HindIII AAGCTT 2 cut(s) 2243, 4145
HpaII CCGG 8 cut(s) 784, 1014, 1734, 1774, 1862, 2537, 2593, 3850
HphI GGTGA 4 cut(s) 580, 1271, 3023, 3910
Hpy99I CGWCG 1 cut(s) 22
HpyAV CCTTC 6 cut(s) 383, 2807, 2910, 3524, 3785, 3847
HpyCH4III ACNGT 7 cut(s) 625, 725, 804, 2409, 3343, 3927, 4252
HpyCH4IV ACGT 7 cut(s) 186, 294, 2542, 3045, 3055, 3276, 3721
HpySE526I ACGT 7 cut(s) 186, 294, 2542, 3045, 3055, 3276, 3721
Hsp92I GRCGYC 1 cut(s) 3045
HspAI GCGC 4 cut(s) 170, 1604, 1858, 2401
KpnI GGTACC 1 cut(s) 2051
Ksp22I TGATCA 2 cut(s) 2467, 2476
MaeII ACGT 7 cut(s) 186, 294, 2542, 3045, 3055, 3276, 3721
MaeIII GTNAC 8 cut(s) 1294, 1427, 1571, 1652, 1659, 1882, 3722, 3799
MfeI CAATTG 3 cut(s) 1599, 3816, 4115
MhlI GDGCHC 7 cut(s) 935, 1225, 2764, 3411, 3748, 3939, 4098
MlyI GAGTC 4 cut(s) 37, 2116, 2681, 3668
MmeI TCCRAC 5 cut(s) 520, 1762, 2865, 2931, 3738
MroXI GAANNNNTTC 4 cut(s) 1171, 2205, 2270, 3905
MslI CAYNNNNRTG 4 cut(s) 1485, 3355, 3840, 4044
MspA1I CMGCKG 6 cut(s) 1205, 1666, 2843, 3484, 3620, 4018
MspCI CTTAAG 1 cut(s) 4070
MspI CCGG 8 cut(s) 784, 1014, 1734, 1774, 1862, 2537, 2593, 3850
MspR9I CCNGG 9 cut(s) 1437, 1577, 1734, 1862, 1997, 2427, 2593, 2946, 3219
MunI CAATTG 3 cut(s) 1599, 3816, 4115
Mva1269I GAATGC 2 cut(s) 3214, 3274
MvaI CCWGG 6 cut(s) 1437, 1577, 1997, 2427, 2946, 3219
NciI CCSGG 3 cut(s) 1734, 1862, 2593
NcoI CCATGG 1 cut(s) 3748
NdeI CATATG 1 cut(s) 91
NmuCI GTSAC 4 cut(s) 1427, 1571, 1882, 3799
NsbI TGCGCA 2 cut(s) 171, 1605
NspI RCATGY 6 cut(s) 64, 645, 1532, 2230, 2987, 3358
PaeR7I CTCGAG 3 cut(s) 1935, 2061, 2730
PagI TCATGA 2 cut(s) 3556, 3835
PasI CCCWGGG 1 cut(s) 2945
PceI AGGCCT 1 cut(s) 1379
PciI ACATGT 3 cut(s) 641, 1528, 3354
PcsI WCGNNNNNNNCGW 1 cut(s) 2068
PctI GAATGC 2 cut(s) 3214, 3274
PdmI GAANNNNTTC 4 cut(s) 1171, 2205, 2270, 3905
PflMI CCANNNNNTGG 2 cut(s) 1256, 2411
PfoI TCCNGGA 2 cut(s) 1435, 1995
PleI GAGTC 4 cut(s) 37, 2116, 2680, 3667
PmaCI CACGTG 1 cut(s) 2543
PmlI CACGTG 1 cut(s) 2543
PpsI GAGTC 4 cut(s) 37, 2116, 2680, 3667
Ppu21I YACGTR 3 cut(s) 295, 2543, 3722
PpuMI RGGWCCY 1 cut(s) 3562
PscI ACATGT 3 cut(s) 641, 1528, 3354
PshBI ATTAAT 1 cut(s) 3984
PsiI TTATAA 1 cut(s) 191
Psp124BI GAGCTC 1 cut(s) 935
Psp1406I AACGTT 1 cut(s) 186
Psp5II RGGWCCY 1 cut(s) 3562
Psp6I CCWGG 6 cut(s) 1435, 1575, 1995, 2425, 2944, 3217
PspCI CACGTG 1 cut(s) 2543
PspGI CCWGG 6 cut(s) 1435, 1575, 1995, 2425, 2944, 3217
PspOMI GGGCCC 1 cut(s) 3744
PspPI GGNCC 7 cut(s) 341, 466, 559, 2456, 3562, 3744, 3745
PspPPI RGGWCCY 1 cut(s) 3562
PspXI VCTCGAGB 1 cut(s) 1935
PstI CTGCAG 2 cut(s) 833, 952
PstNI CAGNNNCTG 1 cut(s) 2789
PvuII CAGCTG 4 cut(s) 1205, 1666, 2843, 3620
RsaI GTAC 8 cut(s) 371, 648, 882, 1366, 2049, 2818, 2934, 3767
RsaNI GTAC 8 cut(s) 370, 647, 881, 1365, 2048, 2817, 2933, 3766
RseI CAYNNNNRTG 4 cut(s) 1485, 3355, 3840, 4044
SacI GAGCTC 1 cut(s) 935
Sau96I GGNCC 7 cut(s) 341, 466, 559, 2456, 3562, 3744, 3745
SchI GAGTC 4 cut(s) 37, 2116, 2681, 3668
ScrFI CCNGG 9 cut(s) 1437, 1577, 1734, 1862, 1997, 2427, 2593, 2946, 3219
SduI GDGCHC 7 cut(s) 935, 1225, 2764, 3411, 3748, 3939, 4098
SfcI CTRYAG 3 cut(s) 829, 948, 1754
Sfr274I CTCGAG 3 cut(s) 1935, 2061, 2730
SinI GGWCC 3 cut(s) 341, 2456, 3562
SlaI CTCGAG 3 cut(s) 1935, 2061, 2730
SmiI ATTTAAAT 1 cut(s) 222
SmiMI CAYNNNNRTG 4 cut(s) 1485, 3355, 3840, 4044
SmlI CTYRAG 8 cut(s) 1935, 2061, 2730, 2924, 3317, 3727, 3769, 4070
SmoI CTYRAG 8 cut(s) 1935, 2061, 2730, 2924, 3317, 3727, 3769, 4070
SseBI AGGCCT 1 cut(s) 1379
SsiI CCGC 4 cut(s) 2045, 3162, 3484, 4016
SspI AATATT 2 cut(s) 448, 3503
SstI GAGCTC 1 cut(s) 935
StuI AGGCCT 1 cut(s) 1379
StyD4I CCNGG 9 cut(s) 1435, 1575, 1732, 1860, 1995, 2425, 2591, 2944, 3217
StyI CCWWGG 5 cut(s) 815, 1449, 1537, 2261, 3748
SwaI ATTTAAAT 1 cut(s) 222
TaaI ACNGT 7 cut(s) 625, 725, 804, 2409, 3343, 3927, 4252
TaiI ACGT 7 cut(s) 189, 297, 2545, 3048, 3058, 3279, 3724
TaqII GACCGA 1 cut(s) 524
TatI WGTACW 3 cut(s) 369, 880, 3765
TseFI GTSAC 4 cut(s) 1427, 1571, 1882, 3799
Tsp45I GTSAC 4 cut(s) 1427, 1571, 1882, 3799
TspGWI ACGGA 1 cut(s) 974
Van91I CCANNNNNTGG 2 cut(s) 1256, 2411
Vha464I CTTAAG 1 cut(s) 4070
VneI GTGCAC 1 cut(s) 3935
VpaK11BI GGWCC 3 cut(s) 341, 2456, 3562
VspI ATTAAT 1 cut(s) 3984
XagI CCTNNNNNAGG 3 cut(s) 2336, 3296, 3779
XapI RAATTY 7 cut(s) 217, 1421, 2067, 2192, 2298, 2376, 3902
XbaI TCTAGA 3 cut(s) 2383, 2605, 3778
XceI RCATGY 6 cut(s) 64, 645, 1532, 2230, 2987, 3358
XhoI CTCGAG 3 cut(s) 1935, 2061, 2730
XmaJI CCTAGG 1 cut(s) 2261
XmiI GTMKAC 2 cut(s) 2519, 2968
XmnI GAANNNNTTC 4 cut(s) 1171, 2205, 2270, 3905
ZraI GACGTC 1 cut(s) 3046
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.