Rh4CG460400

Serine threonine-protein kinase sepA-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0006663
Physical Location & Seq
Forward (+)
69118900 .. 69131990
13091 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG460400.1

Sequence Viewer

Length: 4272 bp
ATGTCTCGGCAGGCGGCGTCGGCGTCGGCCCACTTCCACAAATCCAAGACTCTCGATAACAAATACATGCTTGGAGATGAGATTGGAAAGGGAGCATATGGTCGAGTTTACAAGGGTTTGGATTTGGAGAATGGAGACTTTGTTGCAATTAAACAGGTTTCGTTGGAGAATATTGCGCAGGAGGATCTCAACGTTATAATGCAAGAGATTGATTTACTCAAGAATTTAAATCACAAAAACATTGTGAAATATCTTGGATCCTTAAAGACAAGGACACATCTTCACATAGTCCTAGAATATGTGGAGAATGGCTCACTTGCAAACATTATTAAGCCAAATAAGTTTGGACCTTTTCCAGAATCACTGGTAGCTGTGTACATTGCTCAGGTTTTGGAAGGCTTGGTGTATTTACATGAACAGGGTGTAATCCATCGGGATATCAAGGGTGCAAATATATTGACAACTAAAGAGGGCCTTGTGAAACTTGCTGATTTTGGTGTTGCTACAAAATTGACCGAGGCTGATGTGAATACACATTCAGTTGTTGGAACGCCATATTGGATGGCCCCAGAGGTGATTGAAATGTCGGGGGTTTGTGCTGCTTCAGATATTTGGAGTGTTGGCTGCACTGTTATTGAACTTCTTACATGTGTACCTCCTTATTATGATTTACAGCCAATGCCAGCACTATTTCGTATTGTTCAGGATGAACATCCTCCGATTCCTGACAGTCTATCTCCTGACATTACTGATTTTCTGTGTCAATGCTTTAAAAAGGATGCTAGGCACCGGCCTGATGCAAAAACACTGCTATCTCATCCTTGGATACAAAACTGCAGGCGAGCGTTGCAATCATCTATTCGTCACAGTGGAACATTAAGAGATATACAGGAAGATGTTTCTAAGGGTGCAGAACTCTCCAATGGAGATATTCGGAGCTCTGGCGAAAGTCCTGCAGAGAAAACAGAAGAAGCAGCTTCTGCTATTAAAGCAGACTCTAGGAAAGAGTTATTATCAACCGGGGTTTTTGATATAAGAAAATCTGGCGAGGATCCTGCTTCAGATGTGAAATTTGTTGAGGAAAAAGCAGATGCTTTAGAAAATGATCTGACGGATCAGGCTCCCACCTTAGCTATACATGAAAAATCATCTTTGCCAAATGGTTCTGGCAGAATATCTTCTAATAAAGAATTGTCTGCCTCCAAGCCAGCTGAGCTTGATGGTCGAGCACATACGGCCAACGATGATGAACCACCGATGAATGGGGAGGTTAAATCTCCTGAATTAACGACAAATAGTGTAACTGGGAAACATGGAAAAGGGAATTCTATTGGGTTTAGATCATTTGGTTTTGGAGCAAGAAATCAGGATGGTACTTTTCAAAAGGCTGCAAAGATGCCAGTCAGTTTGGAAGGGAATGAACTCAGTAAATTTAGTGACACTCCAGGAGATGCTTCCTTGGATGATCTATTTCATCCATTGGATAAGCATCCCGATGATAGGGCAACTGAAGCTTCAACATCTTCTTCAATTTCACATTTGAACCAAGGAAATACATCTGTAAATGATGCTGGGAAAAGTGACCTGGCTATGAAATTAAGGGCTACAATTGCTCAGAAGCAAATGGAGAGTGAAATGGGTCAGGCTAATGGTAGTGGTGGTAACCTGTTACAGCTGATGATGGGTGTTCTCCAAGATGATGTGATTGATATTGGTGGTTTGGTATTTGATGAAAAGTTGCCCGGAGAAAACCTTTTTCCCCTACAGGCTGTTGAGTTTAGCCGGTTGGTTGGATCATTGAAACTAGATGAATCAGAAGATGTTGTTGTCTCTGCCTGCCAGAAACTTATTGCTATATTCCATCAGCGTCCAGAGCAGAAAATTGTTTTTGTCACACAGCATGGTTTGCTCCCTTTAATGGAATTGCTTGAAGTTCCTAAAACTCGAGTTATATGTTCTGTGCTTCAAATCATAAACCAGATAATCAAAGACAACACTGATTTCCAGGAAAATGCTTGTCATGTTGGTCTGATCCCAGTGGTAATGAGCTTTGCGGTACCTAATCATTCTCGAGAAATTCGTATGGAAGCAGCTTATTTCTTGCAGCAGCTTTGTCAGTCAAGCCCATTGACTTTGCAAATGTTCATAGCTTGCCGTGGAATACCTGTTCTGGTGGGCTTTTTAGAGGCTGATTATGCAAAATTCAGGGAAATGGTTCATCTAGCTATTGACGGCATGTGGCAAGTCTTTAAGCTTCAGCGATCAACCCCTAGGAATGATTTCTGTCGCATAGCTGCGAAGAATGGAATACTCCTGAGGCTTATTAACACCCTTTATAGCTTGAATGAGGCAACACGTTTAGCTTCCATTTCTGGTGGGGGTGGATTTCCTCTAGACGGTTCTGCTCAGCGCCCACGGTCTGGTTCATTGGATTCTGGTCATCCCATTTTTGCTCAGAGTGATGGACCACTTCCTGATCATAATGATCACTCTAAGGTTAGGCATGGTGTAAATGATTTGCATTTGTCTACCGGAACAGAACCTGCACGTGCTTCAACTTCTAATTCTCAAAGGTCAGATGCCAATCAATCAGATCTGCGGTATCATCATCTAGATACTGATAGACCTCAATCTAGCAATGCAGTAGTAGATGCTTCAGTTTCTTCTAAATTGCAAGACCCAACATCCGCAGATAAAGTAGTCAATATTTCAGCGAAGGAACCTTCAACTACTTCTCGAGGAGATCTTGACCTTAGGCAGCAGCGAGCCCCTATATCTCTTCATAGATCATCCACTGATAGATCTCCTAAAATGATGGAAGCTACATCAAATGGCTTATCAACAACAGCACCTACTCAACAGGAGCAAGTTCGACCTCTTCTTAGCTTGTTGGAGAAAGAGCCCCCTTCAAGACACTTTTCTGGTCAGCTCGATTATGTACGCCACCTCCCTGGGTTGGAGAGACATGAAAGTATACTGCCCCTTTTACATGCATCTAATGAAAAGAAAACAAATGGTGAATTAGATTTCCTTATGGCAGAATTTGCAGATGTCTCTCAACGTGGTAGAGAGAAAGGGAATCTTGATTCTACTACTAGAATTCCCCCCAAAACAGTAAATAAGGAGGTGGGAACACTAGCATCTAACAAAGGAGCTGCTTCCACCTCTGGGATAGCATCTCAGACAGCATCAGGGGTGTTGTCTGGTTCGGGTGTTCTAAATGCTAGGCCAGGCAGTGCAACATCATCAGGTTTACTCTCGCACATGGTTTCGACATTGAATGCAGACGTTGCAAGGGAATACCTAGAAAAGGTGGCAGATCTTCTGCTTGAGTTTGCACGAGCAGATACTACTGTAAAATCATACATGTGTAGCCAAAGTTTGCTCAGCCGTCTTTTTCAGATGTTTAATAGGGTGGAGCCCCCAATTCTTTTAAAGATACTAAAGTGTGTCAACCATTTATCAACCGATCCAAACTGCTTAGAGAATCTTCAGCGGGCAGATGCAATCAAATATCTGATTCCAAATCTTGAACTCAAAGAAGGATCTCTTGTATCTCAAATACATCATGAGGTCCTAAATGCCTTGTTCAATCTGTGTAAGATTAACAAGAGGAGACAGGAACAAGCAGCTGAGAATGGAATAATTCCACACTTAATGCACTTTATTGAGTCTAACTCTCCTCTGAAACAATATGCGTTGCCTTTGCTCTGTGATATGGCTCATGCTTCACGTAATTCAAGAGAGCAATTAAGGGCTCATGGTGGGTTGGATGTGTACTTGAGCCTTCTAGAGGATGAGCTTTGGTCTGTGACTGCATTGGATTCAATTGCTGTTTGCTTAGCTCATGACAATGACAACCGGAAGGTAGAACAAGCACTGTTGAAAAAGGATGCAGTTCAGAAATTGGTGAAATTCTTCCAGTGCTGTCCAGAACAGTATTTCGTGCACATATTGGAGCCATTTTTGAAAATTATCACGAAATCGTCTCGAATTAATACAACATTAGCTGTTAATGGTTTGACACCACTGCTTATCACAAGACTTGACCATCAGGATGCTATTGCCCGTCTAAATCTTCTTAAACTGATAAAGGCTGTTTATGAGCACCACCCACGGCCAAAGCAATTGATTGTGGAGAATGATCTGCCTCAGAAGCTTCGGAATCTAATAGAGGAACGCAGAGATGGGCAACGTTCTGGGGGCCAAGTATTGGTGAAACAAATGGCTACCTCCTTGCTGAAAGCACTCCATATTAACACCGTATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000278 GO:0000281 GO:0000910 GO:0001558 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005813 GO:0005815 GO:0005829 GO:0005856 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007049 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0008361 GO:0009893 GO:0009987 GO:0010324 GO:0010562 GO:0010604 GO:0015630 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022402 GO:0022603 GO:0022604 GO:0023014 GO:0023052 GO:0030587 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032147 GO:0032268 GO:0032270 GO:0032502 GO:0032506 GO:0032535 GO:0033554 GO:0033674 GO:0035556 GO:0036089 GO:0036211 GO:0040008 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0045995 GO:0046777 GO:0048518 GO:0048522 GO:0048638 GO:0048856 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051246 GO:0051247 GO:0051301 GO:0051302 GO:0051338 GO:0051347 GO:0051510 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0061024 GO:0061387 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090066 GO:0090702 GO:0099024 GO:0099120 GO:0140096 GO:1901564 GO:1903047 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1423

Amino Acids

156.42

Weight (kDa)

6.21

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 23 - 276 5.3e-73 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 24 - 272 7e-51 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 197
Acc16I TGCGCA 1 cut(s) 177
Acc36I ACCTGC 1 cut(s) 2551
Acc65I GGTACC 1 cut(s) 2056
AccB1I GGYRCC 2 cut(s) 786, 2056
AccB7I CCANNNNNTGG 2 cut(s) 2420, 4213
AccI GTMKAC 2 cut(s) 2528, 2974
AciI CCGC 5 cut(s) 14, 2054, 2599, 2688, 3496
AclI AACGTT 2 cut(s) 192, 4195
AcoI YGGCCR 2 cut(s) 1236, 4118
AcsI RAATTY 9 cut(s) 223, 1070, 1324, 1430, 2076, 2201, 3041, 3099, 3914
AcuI CTGAAG 6 cut(s) 588, 1044, 1530, 2240, 2640, 3476
AcvI CACGTG 1 cut(s) 2549
AcyI GRCGYC 2 cut(s) 17, 23
AfaI GTAC 6 cut(s) 377, 654, 1375, 2058, 2940, 3779
AflIII ACRYGT 3 cut(s) 647, 2354, 3366
AjnI CCWGG 5 cut(s) 1444, 1584, 2004, 2950, 3229
AjuI GAANNNNNNNTTGG 2 cut(s) 541, 573
AloI GAACNNNNNNTCC 2 cut(s) 2151, 2183
Alw21I GWGCWC 4 cut(s) 941, 1231, 3951, 4110
Alw26I GTCTC 7 cut(s) 9, 129, 1834, 2956, 3058, 3610, 3993
Alw44I GTGCAC 1 cut(s) 3947
AlwNI CAGNNNCTG 2 cut(s) 980, 2543
Ama87I CYCGRG 3 cut(s) 1944, 2070, 2736
AoxI GGCC 8 cut(s) 27, 472, 564, 791, 1236, 3227, 4118, 4204
ApaLI GTGCAC 1 cut(s) 3947
ApoI RAATTY 9 cut(s) 223, 1070, 1324, 1430, 2076, 2201, 3041, 3099, 3914
AseI ATTAAT 1 cut(s) 3996
Asp700I GAANNNNTTC 4 cut(s) 1177, 2214, 2279, 3917
Asp718I GGTACC 1 cut(s) 2056
AspA2I CCTAGG 1 cut(s) 2270
AspLEI GCGC 2 cut(s) 178, 2412
AspS9I GGNCC 7 cut(s) 28, 347, 472, 565, 2465, 3574, 4204
AsuC2I CCSGG 2 cut(s) 1021, 1743
AsuHPI GGTGA 4 cut(s) 586, 3029, 3922, 4228
AvaI CYCGRG 3 cut(s) 1944, 2070, 2736
AvaII GGWCC 3 cut(s) 347, 2465, 3574
AvrII CCTAGG 1 cut(s) 2270
AxyI CCTNAGG 2 cut(s) 2315, 2753
BaeGI GKGCMC 1 cut(s) 3951
BaeI ACNNNNGTAYC 6 cut(s) 636, 669, 3339, 3372, 3431, 3464
BamHI GGATCC 2 cut(s) 257, 1051
BanI GGYRCC 2 cut(s) 786, 2056
BanII GRGCYC 5 cut(s) 941, 2770, 2904, 3423, 3760
BauI CACGAG 1 cut(s) 3339
BbrPI CACGTG 1 cut(s) 2549
Bbv12I GWGCWC 4 cut(s) 941, 1231, 3951, 4110
BceAI ACGGC 5 cut(s) 1251, 2139, 2248, 3375, 4133
BcgI CGANNNNNNTGC 4 cut(s) 935, 969, 1037, 1071
BciT130I CCWGG 5 cut(s) 1446, 1586, 2006, 2952, 3231
BciVI GTATCC 1 cut(s) 819
BclI TGATCA 2 cut(s) 2476, 2485
BcnI CCSGG 2 cut(s) 1021, 1743
BcoDI GTCTC 7 cut(s) 9, 129, 1834, 2956, 3058, 3610, 3993
BfmI CTRYAG 3 cut(s) 835, 954, 1763
BfoI RGCGCY 1 cut(s) 2413
BfuAI ACCTGC 1 cut(s) 2551
BfuI GTATCC 1 cut(s) 819
BglII AGATCT 4 cut(s) 2593, 2743, 2801, 3319
BlnI CCTAGG 1 cut(s) 2270
BlpI GCTNAGC 4 cut(s) 1212, 2406, 3386, 3841
Bme1390I CCNGG 7 cut(s) 1021, 1446, 1586, 1743, 2006, 2952, 3231
Bme18I GGWCC 3 cut(s) 347, 2465, 3574
BmeT110I CYCGRG 3 cut(s) 1944, 2070, 2736
BmgT120I GGNCC 7 cut(s) 28, 347, 472, 565, 2465, 3574, 4204
BmrFI CCNGG 7 cut(s) 1021, 1446, 1586, 1743, 2006, 2952, 3231
BmrI ACTGGG 2 cut(s) 1314, 2030
BmuI ACTGGG 2 cut(s) 1314, 2030
BplI GAGNNNNNCTC 4 cut(s) 296, 328, 3662, 3694
BpmI CTGGAG 1 cut(s) 1428
Bpu10I CCTNAGC 2 cut(s) 384, 1129
Bpu1102I GCTNAGC 4 cut(s) 1212, 2406, 3386, 3841
BpuEI CTTGAG 3 cut(s) 203, 3350, 3802
BpuMI CCSGG 2 cut(s) 1021, 1743
BsaAI YACGTR 2 cut(s) 2549, 3734
BsaBI GATNNNNATC 3 cut(s) 711, 1488, 2583
BsaHI GRCGYC 2 cut(s) 17, 23
BsaWI WCCGGW 2 cut(s) 2531, 3861
BsaXI ACNNNNNCTCC 4 cut(s) 126, 156, 2931, 2961
Bse118I RCCGGY 2 cut(s) 789, 1782
Bse1I ACTGG 5 cut(s) 369, 1309, 1400, 2036, 3922
Bse21I CCTNAGG 2 cut(s) 2315, 2753
Bse3DI GCAATG 2 cut(s) 378, 2644
Bse8I GATNNNNATC 3 cut(s) 711, 1488, 2583
BseBI CCWGG 5 cut(s) 1446, 1586, 2006, 2952, 3231
BseJI GATNNNNATC 3 cut(s) 711, 1488, 2583
BseMI GCAATG 2 cut(s) 378, 2644
BseNI ACTGG 5 cut(s) 369, 1309, 1400, 2036, 3922
BseRI GAGGAG 3 cut(s) 2754, 3628, 3672
BseSI GKGCMC 1 cut(s) 3951
BseYI CCCAGC 1 cut(s) 1571
BsgI GTGCAG 3 cut(s) 610, 930, 2529
BshFI GGCC 8 cut(s) 29, 474, 566, 793, 1238, 3229, 4120, 4206
BshNI GGYRCC 2 cut(s) 786, 2056
BsiHKAI GWGCWC 4 cut(s) 941, 1231, 3951, 4110
BsiHKCI CYCGRG 3 cut(s) 1944, 2070, 2736
BsiSI CCGG 6 cut(s) 790, 1020, 1743, 1783, 2532, 3862
BsmAI GTCTC 7 cut(s) 9, 129, 1834, 2956, 3058, 3610, 3993
BsmBI CGTCTC 1 cut(s) 3993
BsmI GAATGC 1 cut(s) 3286
BsnI GGCC 8 cut(s) 29, 474, 566, 793, 1238, 3229, 4120, 4206
BsoBI CYCGRG 3 cut(s) 1944, 2070, 2736
Bsp1286I GDGCHC 8 cut(s) 941, 1231, 2770, 2904, 3423, 3760, 3951, 4110
Bsp1407I TGTACA 1 cut(s) 375
Bsp1720I GCTNAGC 4 cut(s) 1212, 2406, 3386, 3841
BspACI CCGC 5 cut(s) 14, 2054, 2599, 2688, 3496
BspANI GGCC 8 cut(s) 29, 474, 566, 793, 1238, 3229, 4120, 4206
BspHI TCATGA 2 cut(s) 3568, 3847
BspMAI CTGCAG 2 cut(s) 839, 958
BspMI ACCTGC 1 cut(s) 2551
BspT107I GGYRCC 2 cut(s) 786, 2056
BsrDI GCAATG 2 cut(s) 378, 2644
BsrFI RCCGGY 2 cut(s) 789, 1782
BsrGI TGTACA 1 cut(s) 375
BsrI ACTGG 5 cut(s) 369, 1309, 1400, 2036, 3922
BssAI RCCGGY 2 cut(s) 789, 1782
BssNAI GTATAC 1 cut(s) 2975
BssNI GRCGYC 2 cut(s) 17, 23
BssSI CACGAG 1 cut(s) 3339
BssT1I CCWWGG 4 cut(s) 821, 1458, 1546, 2270
Bst1107I GTATAC 1 cut(s) 2975
Bst2BI CACGAG 1 cut(s) 3339
Bst2UI CCWGG 5 cut(s) 1446, 1586, 2006, 2952, 3231
Bst6I CTCTTC 2 cut(s) 2784, 2883
BstACI GRCGYC 2 cut(s) 17, 23
BstAPI GCANNNNNTGC 4 cut(s) 980, 1906, 3044, 3290
BstAUI TGTACA 1 cut(s) 375
BstBAI YACGTR 2 cut(s) 2549, 3734
BstC8I GCNNGC 9 cut(s) 12, 684, 839, 843, 1209, 1837, 2152, 2766, 3498
BstDSI CCRYGG 3 cut(s) 2155, 2414, 4115
BstEII GGTNACC 1 cut(s) 1661
BstENI CCTNNNNNAGG 2 cut(s) 3308, 3791
BstH2I RGCGCY 1 cut(s) 2413
BstHHI GCGC 2 cut(s) 178, 2412
BstMAI GTCTC 7 cut(s) 9, 129, 1834, 2956, 3058, 3610, 3993
BstNI CCWGG 5 cut(s) 1446, 1586, 2006, 2952, 3231
BstNSI RCATGY 5 cut(s) 70, 651, 2239, 2993, 3370
BstPI GGTNACC 1 cut(s) 1661
BstSCI CCNGG 7 cut(s) 1019, 1444, 1584, 1741, 2004, 2950, 3229
BstSFI CTRYAG 3 cut(s) 835, 954, 1763
BstSLI GKGCMC 1 cut(s) 3951
BstX2I RGATCY 8 cut(s) 184, 257, 1051, 2593, 2743, 2801, 3319, 3545
BstXI CCANNNNNNTGG 1 cut(s) 2951
BstYI RGATCY 8 cut(s) 184, 257, 1051, 2593, 2743, 2801, 3319, 3545
BstZ17I GTATAC 1 cut(s) 2975
Bsu36I CCTNAGG 2 cut(s) 2315, 2753
BsuI GTATCC 1 cut(s) 819
BsuRI GGCC 8 cut(s) 29, 474, 566, 793, 1238, 3229, 4120, 4206
BtgI CCRYGG 3 cut(s) 2155, 2414, 4115
BtsI GCAGTG 3 cut(s) 806, 3241, 4028
BveI ACCTGC 1 cut(s) 2551
Cac8I GCNNGC 9 cut(s) 12, 684, 839, 843, 1209, 1837, 2152, 2766, 3498
CaiI CAGNNNCTG 2 cut(s) 980, 2543
CciI TCATGA 2 cut(s) 3568, 3847
CfoI GCGC 2 cut(s) 178, 2412
Cfr10I RCCGGY 2 cut(s) 789, 1782
Cfr13I GGNCC 7 cut(s) 28, 347, 472, 565, 2465, 3574, 4204
CseI GACGC 3 cut(s) 6, 12, 1856
Csp6I GTAC 6 cut(s) 376, 653, 1374, 2057, 2939, 3778
CviQI GTAC 6 cut(s) 376, 653, 1374, 2057, 2939, 3778
DraI TTTAAA 3 cut(s) 228, 772, 3435
EaeI YGGCCR 2 cut(s) 1236, 4118
Eam1104I CTCTTC 2 cut(s) 2784, 2883
EarI CTCTTC 2 cut(s) 2784, 2883
Ecl136II GAGCTC 1 cut(s) 939
Eco130I CCWWGG 4 cut(s) 821, 1458, 1546, 2270
Eco24I GRGCYC 5 cut(s) 941, 2770, 2904, 3423, 3760
Eco32I GATATC 1 cut(s) 439
Eco47I GGWCC 3 cut(s) 347, 2465, 3574
Eco53kI GAGCTC 1 cut(s) 939
Eco57I CTGAAG 6 cut(s) 588, 1044, 1530, 2240, 2640, 3476
Eco72I CACGTG 1 cut(s) 2549
Eco81I CCTNAGG 2 cut(s) 2315, 2753
Eco88I CYCGRG 3 cut(s) 1944, 2070, 2736
Eco91I GGTNACC 1 cut(s) 1661
EcoICRI GAGCTC 1 cut(s) 939
EcoNI CCTNNNNNAGG 2 cut(s) 3308, 3791
EcoO109I RGGNCCY 2 cut(s) 472, 3574
EcoO65I GGTNACC 1 cut(s) 1661
EcoRI GAATTC 2 cut(s) 1324, 3099
EcoRII CCWGG 5 cut(s) 1444, 1584, 2004, 2950, 3229
EcoRV GATATC 1 cut(s) 439
EcoT14I CCWWGG 4 cut(s) 821, 1458, 1546, 2270
EcoT22I ATGCAT 1 cut(s) 2995
EcoT38I GRGCYC 5 cut(s) 941, 2770, 2904, 3423, 3760
ErhI CCWWGG 4 cut(s) 821, 1458, 1546, 2270
Esp3I CGTCTC 1 cut(s) 3993
FalI AAGNNNNNCTT 6 cut(s) 459, 491, 3066, 3098, 3534, 3566
FauI CCCGC 1 cut(s) 3489
FauNDI CATATG 1 cut(s) 97
FbaI TGATCA 2 cut(s) 2476, 2485
FblI GTMKAC 2 cut(s) 2528, 2974
FriOI GRGCYC 5 cut(s) 941, 2770, 2904, 3423, 3760
FspI TGCGCA 1 cut(s) 177
GlaI GCGC 2 cut(s) 177, 2411
GsaI CCCAGC 1 cut(s) 1575
GsuI CTGGAG 1 cut(s) 1428
HaeII RGCGCY 1 cut(s) 2413
HaeIII GGCC 8 cut(s) 29, 474, 566, 793, 1238, 3229, 4120, 4206
HapII CCGG 6 cut(s) 790, 1020, 1743, 1783, 2532, 3862
HgaI GACGC 3 cut(s) 6, 12, 1856
HhaI GCGC 2 cut(s) 178, 2412
Hin1I GRCGYC 2 cut(s) 17, 23
Hin6I GCGC 2 cut(s) 176, 2410
HinP1I GCGC 2 cut(s) 176, 2410
HincII GTYRAC 1 cut(s) 3454
HindII GTYRAC 1 cut(s) 3454
HindIII AAGCTT 3 cut(s) 1512, 2252, 4157
HpaII CCGG 6 cut(s) 790, 1020, 1743, 1783, 2532, 3862
HphI GGTGA 4 cut(s) 586, 3029, 3922, 4228
Hpy166II GTNNAC 9 cut(s) 109, 376, 653, 2529, 2975, 3254, 3454, 3778, 3949
Hpy8I GTNNAC 9 cut(s) 109, 376, 653, 2529, 2975, 3254, 3454, 3778, 3949
Hpy99I CGWCG 2 cut(s) 22, 28
HpyAV CCTTC 8 cut(s) 389, 1406, 2710, 2733, 2916, 3536, 3797, 3859
HpyCH4IV ACGT 7 cut(s) 192, 2356, 2548, 3061, 3288, 3733, 4195
HpySE526I ACGT 7 cut(s) 192, 2356, 2548, 3061, 3288, 3733, 4195
Hsp92I GRCGYC 2 cut(s) 17, 23
HspAI GCGC 2 cut(s) 176, 2410
KpnI GGTACC 1 cut(s) 2060
Ksp22I TGATCA 2 cut(s) 2476, 2485
LmnI GCTCC 9 cut(s) 92, 936, 1126, 1355, 1914, 2863, 3152, 3418, 3958
MaeII ACGT 7 cut(s) 192, 2356, 2548, 3061, 3288, 3733, 4195
MaeIII GTNAC 8 cut(s) 863, 1300, 1436, 1580, 1661, 1668, 1891, 3811
MfeI CAATTG 3 cut(s) 1608, 3828, 4127
MflI RGATCY 8 cut(s) 184, 257, 1051, 2593, 2743, 2801, 3319, 3545
MhlI GDGCHC 8 cut(s) 941, 1231, 2770, 2904, 3423, 3760, 3951, 4110
MlyI GAGTC 3 cut(s) 43, 989, 3680
MmeI TCCRAC 6 cut(s) 144, 526, 1771, 2871, 2937, 3750
Mph1103I ATGCAT 1 cut(s) 2995
MroXI GAANNNNTTC 4 cut(s) 1177, 2214, 2279, 3917
MslI CAYNNNNRTG 4 cut(s) 1494, 3367, 3852, 4056
MspA1I CMGCKG 4 cut(s) 1211, 1675, 3496, 3632
MspI CCGG 6 cut(s) 790, 1020, 1743, 1783, 2532, 3862
MspR9I CCNGG 7 cut(s) 1021, 1446, 1586, 1743, 2006, 2952, 3231
MunI CAATTG 3 cut(s) 1608, 3828, 4127
Mva1269I GAATGC 1 cut(s) 3286
MvaI CCWGG 5 cut(s) 1446, 1586, 2006, 2952, 3231
NciI CCSGG 2 cut(s) 1021, 1743
NdeI CATATG 1 cut(s) 97
NmuCI GTSAC 5 cut(s) 863, 1436, 1580, 1891, 3811
NsbI TGCGCA 1 cut(s) 177
NsiI ATGCAT 1 cut(s) 2995
NspI RCATGY 5 cut(s) 70, 651, 2239, 2993, 3370
PaeR7I CTCGAG 3 cut(s) 1944, 2070, 2736
PagI TCATGA 2 cut(s) 3568, 3847
PasI CCCWGGG 1 cut(s) 2951
PciI ACATGT 2 cut(s) 647, 3366
PcsI WCGNNNNNNNCGW 1 cut(s) 2077
PctI GAATGC 1 cut(s) 3286
PdmI GAANNNNTTC 4 cut(s) 1177, 2214, 2279, 3917
PflMI CCANNNNNTGG 2 cut(s) 2420, 4213
PfoI TCCNGGA 2 cut(s) 1444, 2004
PleI GAGTC 3 cut(s) 43, 989, 3679
PmaCI CACGTG 1 cut(s) 2549
PmlI CACGTG 1 cut(s) 2549
PpsI GAGTC 3 cut(s) 43, 989, 3679
Ppu21I YACGTR 2 cut(s) 2549, 3734
PpuMI RGGWCCY 1 cut(s) 3574
PscI ACATGT 2 cut(s) 647, 3366
PshBI ATTAAT 1 cut(s) 3996
PsiI TTATAA 1 cut(s) 197
Psp124BI GAGCTC 1 cut(s) 941
Psp1406I AACGTT 2 cut(s) 192, 4195
Psp5II RGGWCCY 1 cut(s) 3574
Psp6I CCWGG 5 cut(s) 1444, 1584, 2004, 2950, 3229
PspCI CACGTG 1 cut(s) 2549
PspEI GGTNACC 1 cut(s) 1661
PspFI CCCAGC 1 cut(s) 1571
PspGI CCWGG 5 cut(s) 1444, 1584, 2004, 2950, 3229
PspPI GGNCC 7 cut(s) 28, 347, 472, 565, 2465, 3574, 4204
PspPPI RGGWCCY 1 cut(s) 3574
PspXI VCTCGAGB 1 cut(s) 1944
PstI CTGCAG 2 cut(s) 839, 958
PstNI CAGNNNCTG 2 cut(s) 980, 2543
PsuI RGATCY 8 cut(s) 184, 257, 1051, 2593, 2743, 2801, 3319, 3545
PvuII CAGCTG 3 cut(s) 1211, 1675, 3632
RsaI GTAC 6 cut(s) 377, 654, 1375, 2058, 2940, 3779
RsaNI GTAC 6 cut(s) 376, 653, 1374, 2057, 2939, 3778
RseI CAYNNNNRTG 4 cut(s) 1494, 3367, 3852, 4056
SacI GAGCTC 1 cut(s) 941
Sau96I GGNCC 7 cut(s) 28, 347, 472, 565, 2465, 3574, 4204
SchI GAGTC 3 cut(s) 43, 989, 3680
ScrFI CCNGG 7 cut(s) 1021, 1446, 1586, 1743, 2006, 2952, 3231
SduI GDGCHC 8 cut(s) 941, 1231, 2770, 2904, 3423, 3760, 3951, 4110
SfcI CTRYAG 3 cut(s) 835, 954, 1763
Sfr274I CTCGAG 3 cut(s) 1944, 2070, 2736
SinI GGWCC 3 cut(s) 347, 2465, 3574
SlaI CTCGAG 3 cut(s) 1944, 2070, 2736
SmiI ATTTAAAT 1 cut(s) 228
SmiMI CAYNNNNRTG 4 cut(s) 1494, 3367, 3852, 4056
SmlI CTYRAG 6 cut(s) 218, 1944, 2070, 2736, 3329, 3781
SmoI CTYRAG 6 cut(s) 218, 1944, 2070, 2736, 3329, 3781
SsiI CCGC 5 cut(s) 14, 2054, 2599, 2688, 3496
SspI AATATT 2 cut(s) 172, 2707
SstI GAGCTC 1 cut(s) 941
StyD4I CCNGG 7 cut(s) 1019, 1444, 1584, 1741, 2004, 2950, 3229
StyI CCWWGG 4 cut(s) 821, 1458, 1546, 2270
SwaI ATTTAAAT 1 cut(s) 228
TaiI ACGT 7 cut(s) 195, 2359, 2551, 3064, 3291, 3736, 4198
TaqII GACCGA 1 cut(s) 530
TatI WGTACW 2 cut(s) 375, 3777
TauI GCSGC 1 cut(s) 17
TseFI GTSAC 5 cut(s) 863, 1436, 1580, 1891, 3811
Tsp45I GTSAC 5 cut(s) 863, 1436, 1580, 1891, 3811
TspGWI ACGGA 1 cut(s) 1127
Van91I CCANNNNNTGG 2 cut(s) 2420, 4213
VneI GTGCAC 1 cut(s) 3947
VpaK11BI GGWCC 3 cut(s) 347, 2465, 3574
VspI ATTAAT 1 cut(s) 3996
XagI CCTNNNNNAGG 2 cut(s) 3308, 3791
XapI RAATTY 9 cut(s) 223, 1070, 1324, 1430, 2076, 2201, 3041, 3099, 3914
XbaI TCTAGA 3 cut(s) 2392, 2611, 3790
XceI RCATGY 5 cut(s) 70, 651, 2239, 2993, 3370
XhoI CTCGAG 3 cut(s) 1944, 2070, 2736
XmaJI CCTAGG 1 cut(s) 2270
XmiI GTMKAC 2 cut(s) 2528, 2974
XmnI GAANNNNTTC 4 cut(s) 1177, 2214, 2279, 3917
Zsp2I ATGCAT 1 cut(s) 2995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.