Rroxscaffold_5G00386940

Serine threonine-protein kinase sepA-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006663
Physical Location & Seq
Forward (+)
65664888 .. 65676906
12019 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386940.1

Sequence Viewer

Length: 4101 bp
ATGTCTCGGCAGGCGGCGTCGGCCCACTTCCACAAATCCAAGACTCTCGATAACAAATACATGCTTGGAGATGAGATTGGAAAGGGAGCATATGGTCGAGTTTACAAGGGTTTGGATTTGGAGAATGGAGACTTTGTTGCAATTAAACAGGTTTCGTTGGAGAATATTGCGCAGGAGGATCTCAACGTTATAATGAATTTAAATCACAAAAACATTGTGAAATATCTTGGATCCTTAAAGACAAAGACACATCTTCACATAGTCCTAGAATATGTGGAGAATGGCTCACTTGCAAACATTATTAAGCCAAATAAGTTTGGACCTTTTCCAGAATCACTGGTAGCTGTGTACATTGCTCAGGTTTTGGAAGGCTTGGTGTATTTACATGAACAGGGTGTAATCCATCGGGATATCAAGGGTGCAAATATATTGACAACTAAAGAGGGCCTTGTGAAACTTGCTGATTTTGGTGTTGCTACAAAATTGACTGAGGCTGATGTGAATACACATTCAGTTGTTGGAACGCCATATTGGATGGCCCCAGAGGTGATTGAAATGTCGGGGGTTTGTGCTGCTTCAGATATTTGGAGTGTTGGCTGCACTGTTATTGAACTTCTTACATGTGTACCTCCTTATTATGATTTACAGCCAATGCCAGCGCTATTTCGTATTGTTCAGGATGAACATCCTCCGATTCCTGACAGTCTATCTCCTGACATTACTGATTTTCTATGTCAATGCTTTAAAAAGTCATACTCCTTCCCTTTATGTCATTGTCTTCAAGTTAGAGATGAGTATGCAAATAGAAGAGGTGAACAAGCAGTGAAGATGTTTCTGTACATGGATGCTAGGCACCGGCCTGATGCAAAAACACTGCTATCTCATCCTTGGATACAAAACTGCAGGCGAGCGTTGCAATCATCTATTCGTCACAGTGGAACATTAAGAGATATACAGGAAGATGTTTCTAAGGGTGCAGAACTCTCCAATGGAGATATTCAGAGCTCTGGCGAAAGTCCTGCAGAGAAAACAGAAGAAGCAGCTTCTGCTATTAAAGCAGACTCTAGGAAAGAGTTATTATCAACTGGGGTTTCTGATATGAGAACATCTGGCGAGGATCCTGCTTCAGATGTGAAATTTGTTGAGGAAAAAGCAGATGCTTTAGAAAATGATCTGACGGATCAGGCTCCCACCTTAGCTATACATGAAAAATCATCTTTGCCAAATGGTTCTGGCAGAATATCTTCTAATAAAGAATTGTCTGCCTCCGAGCCAGCTGAGCTTGATGGTCGAGTACATACAGCCAACGATGATGAACCACCGATGAATGGGGAGGTTAAATCTCCTGAATTAATGACAAATAGTGTAACTGGGAAACATGGAAAAGGGAATTCTATTGGGTTTAGATCATTTGGTTTTGGAGCAAGAAATCAGGATGGTTCTTTTCAAAAGGCTGCAAAGATGCCAGTCAGTTTGGAAGGGAATGAACTCAGTAAATTTAGTGACACTCCAGGAGATGCTTCCTTGGATGATCTATTTCATCCATTGGATAAGCATCCCGATGATAGGGCAACTGAAGCTTCAACATCTTCTTCAATTTCACATTTGAACCAAGGAAATACATCTGTAAATGATGCTGGGAAAAGTGACCTGGCTATGAAATTAAGGGCTACAATTGCTCAGAAGCAAATGGAGAGTGAAATGGGTCAGGCTAATGGTAGTGGTGGTAACCTGTTACAGCTGATGATGGGTGTTCTCCAAGATGATGTGATTGATATTGGTGGTTTGGTATTTGATGAAAAGTTGCCCGGAGAAAACCTTTTTCCCCTACAGGCTGTTGAGTTTAGCCGGTTGGTTGGATCATTGAAACTAGAAGAATCAGAAGATGTTGTTGTCTCTGCCTGCCAGAAACTTATTGCTATTTTCCATCAGCGTCCAGAGCAGAAAATTGTTTTTGTCACACAGCATGGTTTGCTCCCTTTAATGGAATTGCTTGAAGTTCCTAAAACTCGAGTTATATGTTCTGTGCTTCAAATCATAAACCAGATAATCAAAGACAACACTGATTTCCAGGAAAATGCTTGTCATGTTGGTCTGATCCCAGTGGTAATGAGCTTTGCGGTACCTAATCATTCTCGAGAAATTCGTATGGAAGCAGCTTATTTCTTGCAGCAGCTTTGTCAGTCAAGCCCATTGACTTTGCAAATGTTCATAGCTTGCCGTGGAATACCTGTTCTGGTGGGCTTTTTAGAGGCTGATTATGCAAAATTCAGGGAAATGGTTCATCTAGCTATTGACGGCATGTGGCAAGTCTTTAAGCTTCAGCGATCAACCCCTAGGAATGATTTCTGTCGCATAGCTGCGAAGAATGGAATACTCCTGAGGCTTATTAACACCCTTTATAGCTTGAATGAGGCAACACGTTTAGCTTCCATTTCTGGTGGGGGTGGATTTCCTGTAGACGGTTCTGCTCAGCGCCCACGGTCTGGTTCATTGGATTCTGGTCATCCCATTTTTGCTCAGAGTGATGGACCACTTCCTGATCATAATGATCACTCTAAGGTTAGGCATGGTGTAAATGACTTGCATTTGTCTACCGGAACAGAACCTGCACGTGCTTCAACTTCTAATTCTCAAAGGTCAGATGCCAATCAATCAGATCTGCGGTATCATCATCTAGATACTGATAGACCTCAATCTAGCATTGCAGTAGTAGATGCTTCAGTTTCTTCCAAATTGCAAGACCCAACATCCGCAGACAAAGTAGTCAATATTTCAGTGAAGGAACCTTCAACTACTTCTCGAGGAGATCTTGACCTTAGGCAGCAGCGAGCCCCTATATCTCTTCATAGATCATCCACTGATAGATCTCCTAAAATGATGGAAGCTACATCAAATGGCTTATCAACAACAGCACCTACTCAACAGGAGCAGGTTCGACCTCTTCTTAGCTTGTTGGAGAAAGAGCCCCCTTCAAGACACTTTTCTGGTCAGCTCGATTATGTACGCCACCTCCCTGGGTTGGAGAGACATGAAAGTATACTGCCCCTTTTACATGCATCTAATGAAAAGAAAACAAATGGTGAATTAGATTTCCTTATGGCAGAATTTGCAGTAAATAAGGAGGTGGGAACACTAGCATCTAACAAAGGAGCTGCTTCCACCTCTGGGATAGCATCTCAGACAGCATCAGGGGTGTTGTCTGGTTCGGGTGTTCTAAATGCTAGGCCAGGCAGTGCAACATCATCAGGTTTACTCTCGCACATGATACTAAAGTGCGTCAACCATTTATCTACTGATCCAAACTGCTTAGAGAATCTTCAGCGGGCAGATGCAATCAAATATCTGATTCCAAATCTTGAACTCAAAGAAGGATCTCTTGTATCTCAAATACATCATGAGGTCCTAAATGCCTTGTTCAATCTGTGTAAGATTAACAAGAGGAGACAGGAACAAGCAGCTGAGAATGGAATAATTCCACACTTAATGCACTTTATTGAGTCTAACTCTCCTCTGAAACAATATGCGTTGCCTTTGCTCTGTGATATGGCTCATGCTTCACGTAATTCAAGAGAGCAATTAAGGGCTCATGGTGGGTTGGATGTGTACTTGAGCCTTCTAGAGGATGAGCTTTGGTCTGTGACTGCATTGGATTCAATTGCTGTTTGCTTAGCTCATGACAATGACAACCGGAAGGTAGAACAAGCACTGTTGAAAAAGGATGCAGTTCAGAAATTGGTGAAATTCTTCCAGTGCTGTCCAGAACAGTATTTTGTGCACATATTGGAGCCATTTTTGAAAATTATCACGAAATCGTCTCGAATTAATACAACATTAGCTGTTAATGGTTTGACACCACTGCTTATCACAAGACTTGACCATCAGGATGCTATTGCCCGTCTAAATCTTCTTAAACTGATAAAGGCTGTTTATGAGCACCACCCACGGCCAAAGCAATTGATTGTGGAGAATGATCTGCCTCAGAAGCTTCGGAATCTAATAGAGGAACGCAGAGATGGGCAACGTTCTGGGGGCCAAGTATTGGTGAAACAAATGGCTACCTCCTTGCTGAAAGCACTCCATATTAACACCGTATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000278 GO:0000281 GO:0000910 GO:0001558 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005813 GO:0005815 GO:0005829 GO:0005856 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007049 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0008361 GO:0009893 GO:0009987 GO:0010324 GO:0010562 GO:0010604 GO:0015630 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022402 GO:0022603 GO:0022604 GO:0023014 GO:0023052 GO:0030587 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032147 GO:0032268 GO:0032270 GO:0032502 GO:0032506 GO:0032535 GO:0033554 GO:0033674 GO:0035556 GO:0036089 GO:0036211 GO:0040008 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0045995 GO:0046777 GO:0048518 GO:0048522 GO:0048638 GO:0048856 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051246 GO:0051247 GO:0051301 GO:0051302 GO:0051338 GO:0051347 GO:0051510 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0061024 GO:0061387 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090066 GO:0090702 GO:0099024 GO:0099120 GO:0140096 GO:1901564 GO:1903047 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1366

Amino Acids

150.15

Weight (kDa)

6.1

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 21 - 251 3.9e-62 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 22 - 251 1.8e-43 Protein tyrosine and serine/threonine kinase
ARM_ARMC5 PF24768 1090 - 1210 9.3e-07 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 191
AasI GACNNNNNNGTC 1 cut(s) 2765
Acc16I TGCGCA 1 cut(s) 171
Acc36I ACCTGC 2 cut(s) 2617, 2923
Acc65I GGTACC 1 cut(s) 2122
AccB1I GGYRCC 2 cut(s) 852, 2122
AccB7I CCANNNNNTGG 2 cut(s) 2486, 4042
AccI GTMKAC 3 cut(s) 2460, 2594, 3040
AciI CCGC 5 cut(s) 14, 2120, 2665, 2754, 3325
AclI AACGTT 2 cut(s) 186, 4024
AcoI YGGCCR 1 cut(s) 3947
AcsI RAATTY 8 cut(s) 196, 1136, 1390, 1496, 2142, 2267, 3107, 3743
AcuI CTGAAG 6 cut(s) 561, 1110, 1596, 2306, 2706, 3305
AcvI CACGTG 1 cut(s) 2615
AcyI GRCGYC 1 cut(s) 17
AfaI GTAC 7 cut(s) 350, 627, 839, 1296, 2124, 3006, 3608
AfeI AGCGCT 1 cut(s) 660
AfiI CCNNNNNNNGG 9 cut(s) 1328, 1566, 1984, 2462, 2486, 3022, 3168, 3622, 4042
AflIII ACRYGT 2 cut(s) 620, 2420
AjnI CCWGG 5 cut(s) 1510, 1650, 2070, 3016, 3229
AjuI GAANNNNNNNTTGG 2 cut(s) 514, 546
AloI GAACNNNNNNTCC 2 cut(s) 2217, 2249
Alw21I GWGCWC 3 cut(s) 1007, 3780, 3939
Alw26I GTCTC 6 cut(s) 9, 123, 1900, 3022, 3439, 3822
Alw44I GTGCAC 1 cut(s) 3776
AlwNI CAGNNNCTG 2 cut(s) 1046, 2609
Ama87I CYCGRG 3 cut(s) 2010, 2136, 2802
Aor51HI AGCGCT 1 cut(s) 660
AoxI GGCC 7 cut(s) 21, 445, 537, 857, 3227, 3947, 4033
ApaLI GTGCAC 1 cut(s) 3776
ApoI RAATTY 8 cut(s) 196, 1136, 1390, 1496, 2142, 2267, 3107, 3743
AseI ATTAAT 2 cut(s) 1352, 3825
Asp700I GAANNNNTTC 4 cut(s) 1243, 2280, 2345, 3746
Asp718I GGTACC 1 cut(s) 2122
AspA2I CCTAGG 1 cut(s) 2336
AspLEI GCGC 3 cut(s) 172, 661, 2478
AspS9I GGNCC 7 cut(s) 22, 320, 445, 538, 2531, 3403, 4033
AsuC2I CCSGG 1 cut(s) 1809
AsuHPI GGTGA 5 cut(s) 559, 824, 3095, 3751, 4057
AvaI CYCGRG 3 cut(s) 2010, 2136, 2802
AvaII GGWCC 3 cut(s) 320, 2531, 3403
AvrII CCTAGG 1 cut(s) 2336
AxyI CCTNAGG 2 cut(s) 2381, 2819
BaeGI GKGCMC 1 cut(s) 3780
BaeI ACNNNNGTAYC 4 cut(s) 609, 642, 3260, 3293
BamHI GGATCC 2 cut(s) 230, 1117
BanI GGYRCC 2 cut(s) 852, 2122
BanII GRGCYC 4 cut(s) 1007, 2836, 2970, 3589
BbrPI CACGTG 1 cut(s) 2615
BbsI GAAGAC 1 cut(s) 770
Bbv12I GWGCWC 3 cut(s) 1007, 3780, 3939
BceAI ACGGC 3 cut(s) 2205, 2314, 3962
BcgI CGANNNNNNTGC 4 cut(s) 1001, 1035, 1103, 1137
BciT130I CCWGG 5 cut(s) 1512, 1652, 2072, 3018, 3231
BciVI GTATCC 1 cut(s) 885
BclI TGATCA 2 cut(s) 2542, 2551
BcnI CCSGG 1 cut(s) 1809
BcoDI GTCTC 6 cut(s) 9, 123, 1900, 3022, 3439, 3822
BfmI CTRYAG 4 cut(s) 901, 1020, 1829, 2457
BfoI RGCGCY 2 cut(s) 662, 2479
BfuAI ACCTGC 2 cut(s) 2617, 2923
BfuI GTATCC 1 cut(s) 885
BglII AGATCT 3 cut(s) 2659, 2809, 2867
BlnI CCTAGG 1 cut(s) 2336
BlpI GCTNAGC 3 cut(s) 1278, 2472, 3670
Bme1390I CCNGG 6 cut(s) 1512, 1652, 1809, 2072, 3018, 3231
Bme18I GGWCC 3 cut(s) 320, 2531, 3403
BmeT110I CYCGRG 3 cut(s) 2010, 2136, 2802
BmgT120I GGNCC 7 cut(s) 22, 320, 445, 538, 2531, 3403, 4033
BmiI GGNNCC 9 cut(s) 232, 540, 854, 1119, 1188, 2124, 2787, 3789, 4034
BmrFI CCNGG 6 cut(s) 1512, 1652, 1809, 2072, 3018, 3231
BmrI ACTGGG 3 cut(s) 1095, 1380, 2096
BmuI ACTGGG 3 cut(s) 1095, 1380, 2096
BpiI GAAGAC 1 cut(s) 770
BplI GAGNNNNNCTC 4 cut(s) 269, 301, 3491, 3523
BpmI CTGGAG 1 cut(s) 1494
Bpu10I CCTNAGC 2 cut(s) 357, 1195
Bpu1102I GCTNAGC 3 cut(s) 1278, 2472, 3670
BpuEI CTTGAG 1 cut(s) 3631
BpuMI CCSGG 1 cut(s) 1809
BsaAI YACGTR 2 cut(s) 2615, 3563
BsaBI GATNNNNATC 3 cut(s) 684, 1554, 2649
BsaHI GRCGYC 1 cut(s) 17
BsaJI CCNNGG 9 cut(s) 887, 1524, 1612, 2221, 2336, 2480, 3016, 3017, 3944
BsaWI WCCGGW 2 cut(s) 2597, 3690
BsaXI ACNNNNNCTCC 4 cut(s) 120, 150, 2997, 3027
Bsc4I CCNNNNNNNGG 9 cut(s) 1328, 1566, 1984, 2462, 2486, 3022, 3168, 3622, 4042
Bse118I RCCGGY 2 cut(s) 855, 1848
Bse1I ACTGG 6 cut(s) 342, 1090, 1375, 1466, 2102, 3751
Bse21I CCTNAGG 2 cut(s) 2381, 2819
Bse3DI GCAATG 2 cut(s) 351, 2703
Bse8I GATNNNNATC 3 cut(s) 684, 1554, 2649
BseBI CCWGG 5 cut(s) 1512, 1652, 2072, 3018, 3231
BseDI CCNNGG 9 cut(s) 887, 1524, 1612, 2221, 2336, 2480, 3016, 3017, 3944
BseJI GATNNNNATC 3 cut(s) 684, 1554, 2649
BseLI CCNNNNNNNGG 9 cut(s) 1328, 1566, 1984, 2462, 2486, 3022, 3168, 3622, 4042
BseMI GCAATG 2 cut(s) 351, 2703
BseNI ACTGG 6 cut(s) 342, 1090, 1375, 1466, 2102, 3751
BseRI GAGGAG 3 cut(s) 2820, 3457, 3501
BseSI GKGCMC 1 cut(s) 3780
BseYI CCCAGC 1 cut(s) 1637
BsgI GTGCAG 3 cut(s) 583, 996, 2595
BshFI GGCC 7 cut(s) 23, 447, 539, 859, 3229, 3949, 4035
BshNI GGYRCC 2 cut(s) 852, 2122
BsiHKAI GWGCWC 3 cut(s) 1007, 3780, 3939
BsiHKCI CYCGRG 3 cut(s) 2010, 2136, 2802
BsiSI CCGG 5 cut(s) 856, 1809, 1849, 2598, 3691
BslI CCNNNNNNNGG 9 cut(s) 1328, 1566, 1984, 2462, 2486, 3022, 3168, 3622, 4042
BsmAI GTCTC 6 cut(s) 9, 123, 1900, 3022, 3439, 3822
BsmBI CGTCTC 1 cut(s) 3822
BsnI GGCC 7 cut(s) 23, 447, 539, 859, 3229, 3949, 4035
BsoBI CYCGRG 3 cut(s) 2010, 2136, 2802
Bsp1286I GDGCHC 6 cut(s) 1007, 2836, 2970, 3589, 3780, 3939
Bsp1407I TGTACA 2 cut(s) 348, 837
Bsp1720I GCTNAGC 3 cut(s) 1278, 2472, 3670
BspACI CCGC 5 cut(s) 14, 2120, 2665, 2754, 3325
BspANI GGCC 7 cut(s) 23, 447, 539, 859, 3229, 3949, 4035
BspHI TCATGA 2 cut(s) 3397, 3676
BspLI GGNNCC 9 cut(s) 232, 540, 854, 1119, 1188, 2124, 2787, 3789, 4034
BspMAI CTGCAG 2 cut(s) 905, 1024
BspMI ACCTGC 2 cut(s) 2617, 2923
BspT107I GGYRCC 2 cut(s) 852, 2122
BsrDI GCAATG 2 cut(s) 351, 2703
BsrFI RCCGGY 2 cut(s) 855, 1848
BsrGI TGTACA 2 cut(s) 348, 837
BsrI ACTGG 6 cut(s) 342, 1090, 1375, 1466, 2102, 3751
BssAI RCCGGY 2 cut(s) 855, 1848
BssECI CCNNGG 9 cut(s) 887, 1524, 1612, 2221, 2336, 2480, 3016, 3017, 3944
BssNAI GTATAC 1 cut(s) 3041
BssNI GRCGYC 1 cut(s) 17
BssT1I CCWWGG 4 cut(s) 887, 1524, 1612, 2336
Bst1107I GTATAC 1 cut(s) 3041
Bst2UI CCWGG 5 cut(s) 1512, 1652, 2072, 3018, 3231
Bst4CI ACNGT 8 cut(s) 604, 704, 935, 2465, 2484, 3711, 3768, 4093
Bst6I CTCTTC 3 cut(s) 802, 2850, 2949
BstACI GRCGYC 1 cut(s) 17
BstAPI GCANNNNNTGC 3 cut(s) 1046, 1972, 3110
BstAUI TGTACA 2 cut(s) 348, 837
BstBAI YACGTR 2 cut(s) 2615, 3563
BstC8I GCNNGC 9 cut(s) 12, 657, 905, 909, 1275, 1903, 2218, 2832, 3327
BstDSI CCRYGG 3 cut(s) 2221, 2480, 3944
BstEII GGTNACC 1 cut(s) 1727
BstENI CCTNNNNNAGG 1 cut(s) 3620
BstH2I RGCGCY 2 cut(s) 662, 2479
BstHHI GCGC 3 cut(s) 172, 661, 2478
BstMAI GTCTC 6 cut(s) 9, 123, 1900, 3022, 3439, 3822
BstNI CCWGG 5 cut(s) 1512, 1652, 2072, 3018, 3231
BstNSI RCATGY 4 cut(s) 64, 624, 2305, 3059
BstPI GGTNACC 1 cut(s) 1727
BstSCI CCNGG 6 cut(s) 1510, 1650, 1807, 2070, 3016, 3229
BstSFI CTRYAG 4 cut(s) 901, 1020, 1829, 2457
BstSLI GKGCMC 1 cut(s) 3780
BstV2I GAAGAC 1 cut(s) 770
BstX2I RGATCY 7 cut(s) 178, 230, 1117, 2659, 2809, 2867, 3374
BstXI CCANNNNNNTGG 1 cut(s) 3017
BstYI RGATCY 7 cut(s) 178, 230, 1117, 2659, 2809, 2867, 3374
BstZ17I GTATAC 1 cut(s) 3041
Bsu36I CCTNAGG 2 cut(s) 2381, 2819
BsuI GTATCC 1 cut(s) 885
BsuRI GGCC 7 cut(s) 23, 447, 539, 859, 3229, 3949, 4035
BtgI CCRYGG 3 cut(s) 2221, 2480, 3944
BtsI GCAGTG 4 cut(s) 828, 872, 3241, 3857
BveI ACCTGC 2 cut(s) 2617, 2923
Cac8I GCNNGC 9 cut(s) 12, 657, 905, 909, 1275, 1903, 2218, 2832, 3327
CaiI CAGNNNCTG 2 cut(s) 1046, 2609
CciI TCATGA 2 cut(s) 3397, 3676
CfoI GCGC 3 cut(s) 172, 661, 2478
Cfr10I RCCGGY 2 cut(s) 855, 1848
Cfr13I GGNCC 7 cut(s) 22, 320, 445, 538, 2531, 3403, 4033
CseI GACGC 3 cut(s) 6, 1922, 3268
Csp6I GTAC 7 cut(s) 349, 626, 838, 1295, 2123, 3005, 3607
CviQI GTAC 7 cut(s) 349, 626, 838, 1295, 2123, 3005, 3607
DraI TTTAAA 2 cut(s) 201, 745
DrdI GACNNNNNNGTC 1 cut(s) 2765
DseDI GACNNNNNNGTC 1 cut(s) 2765
EaeI YGGCCR 1 cut(s) 3947
Eam1104I CTCTTC 3 cut(s) 802, 2850, 2949
EarI CTCTTC 3 cut(s) 802, 2850, 2949
Ecl136II GAGCTC 1 cut(s) 1005
Eco130I CCWWGG 4 cut(s) 887, 1524, 1612, 2336
Eco24I GRGCYC 4 cut(s) 1007, 2836, 2970, 3589
Eco32I GATATC 1 cut(s) 412
Eco47I GGWCC 3 cut(s) 320, 2531, 3403
Eco47III AGCGCT 1 cut(s) 660
Eco53kI GAGCTC 1 cut(s) 1005
Eco57I CTGAAG 6 cut(s) 561, 1110, 1596, 2306, 2706, 3305
Eco72I CACGTG 1 cut(s) 2615
Eco81I CCTNAGG 2 cut(s) 2381, 2819
Eco88I CYCGRG 3 cut(s) 2010, 2136, 2802
Eco91I GGTNACC 1 cut(s) 1727
EcoICRI GAGCTC 1 cut(s) 1005
EcoNI CCTNNNNNAGG 1 cut(s) 3620
EcoO109I RGGNCCY 2 cut(s) 445, 3403
EcoO65I GGTNACC 1 cut(s) 1727
EcoRI GAATTC 1 cut(s) 1390
EcoRII CCWGG 5 cut(s) 1510, 1650, 2070, 3016, 3229
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 4 cut(s) 887, 1524, 1612, 2336
EcoT22I ATGCAT 1 cut(s) 3061
EcoT38I GRGCYC 4 cut(s) 1007, 2836, 2970, 3589
ErhI CCWWGG 4 cut(s) 887, 1524, 1612, 2336
Esp3I CGTCTC 1 cut(s) 3822
FalI AAGNNNNNCTT 4 cut(s) 432, 464, 3363, 3395
FauI CCCGC 1 cut(s) 3318
FauNDI CATATG 1 cut(s) 91
FbaI TGATCA 2 cut(s) 2542, 2551
FblI GTMKAC 3 cut(s) 2460, 2594, 3040
FriOI GRGCYC 4 cut(s) 1007, 2836, 2970, 3589
FspI TGCGCA 1 cut(s) 171
GlaI GCGC 3 cut(s) 171, 660, 2477
GsaI CCCAGC 1 cut(s) 1641
GsuI CTGGAG 1 cut(s) 1494
HaeII RGCGCY 2 cut(s) 662, 2479
HaeIII GGCC 7 cut(s) 23, 447, 539, 859, 3229, 3949, 4035
HapII CCGG 5 cut(s) 856, 1809, 1849, 2598, 3691
HgaI GACGC 3 cut(s) 6, 1922, 3268
HhaI GCGC 3 cut(s) 172, 661, 2478
Hin1I GRCGYC 1 cut(s) 17
Hin6I GCGC 3 cut(s) 170, 659, 2476
HinP1I GCGC 3 cut(s) 170, 659, 2476
HincII GTYRAC 1 cut(s) 3283
HindII GTYRAC 1 cut(s) 3283
HindIII AAGCTT 3 cut(s) 1578, 2318, 3986
HpaII CCGG 5 cut(s) 856, 1809, 1849, 2598, 3691
HphI GGTGA 5 cut(s) 559, 824, 3095, 3751, 4057
Hpy99I CGWCG 1 cut(s) 22
HpyAV CCTTC 9 cut(s) 362, 769, 1472, 2776, 2799, 2982, 3365, 3626, 3688
HpyCH4III ACNGT 8 cut(s) 604, 704, 935, 2465, 2484, 3711, 3768, 4093
HpyCH4IV ACGT 5 cut(s) 186, 2422, 2614, 3562, 4024
HpySE526I ACGT 5 cut(s) 186, 2422, 2614, 3562, 4024
Hsp92I GRCGYC 1 cut(s) 17
HspAI GCGC 3 cut(s) 170, 659, 2476
KpnI GGTACC 1 cut(s) 2126
Ksp22I TGATCA 2 cut(s) 2542, 2551
LmnI GCTCC 7 cut(s) 86, 1192, 1421, 1980, 2929, 3152, 3787
MaeII ACGT 5 cut(s) 186, 2422, 2614, 3562, 4024
MaeIII GTNAC 8 cut(s) 929, 1366, 1502, 1646, 1727, 1734, 1957, 3640
MfeI CAATTG 3 cut(s) 1674, 3657, 3956
MflI RGATCY 7 cut(s) 178, 230, 1117, 2659, 2809, 2867, 3374
MhlI GDGCHC 6 cut(s) 1007, 2836, 2970, 3589, 3780, 3939
MlyI GAGTC 3 cut(s) 37, 1055, 3509
MmeI TCCRAC 6 cut(s) 138, 499, 1837, 2937, 3003, 3579
Mph1103I ATGCAT 1 cut(s) 3061
MroXI GAANNNNTTC 4 cut(s) 1243, 2280, 2345, 3746
MslI CAYNNNNRTG 3 cut(s) 1560, 3681, 3885
MspA1I CMGCKG 4 cut(s) 1277, 1741, 3325, 3461
MspI CCGG 5 cut(s) 856, 1809, 1849, 2598, 3691
MspR9I CCNGG 6 cut(s) 1512, 1652, 1809, 2072, 3018, 3231
MunI CAATTG 3 cut(s) 1674, 3657, 3956
MvaI CCWGG 5 cut(s) 1512, 1652, 2072, 3018, 3231
NciI CCSGG 1 cut(s) 1809
NdeI CATATG 1 cut(s) 91
NlaIV GGNNCC 9 cut(s) 232, 540, 854, 1119, 1188, 2124, 2787, 3789, 4034
NmuCI GTSAC 5 cut(s) 929, 1502, 1646, 1957, 3640
NsbI TGCGCA 1 cut(s) 171
NsiI ATGCAT 1 cut(s) 3061
NspI RCATGY 4 cut(s) 64, 624, 2305, 3059
PaeR7I CTCGAG 3 cut(s) 2010, 2136, 2802
PagI TCATGA 2 cut(s) 3397, 3676
PasI CCCWGGG 1 cut(s) 3017
PciI ACATGT 1 cut(s) 620
PcsI WCGNNNNNNNCGW 1 cut(s) 2143
PdmI GAANNNNTTC 4 cut(s) 1243, 2280, 2345, 3746
PfeI GAWTC 8 cut(s) 332, 694, 1877, 2498, 3316, 3349, 3653, 3994
PflMI CCANNNNNTGG 2 cut(s) 2486, 4042
PfoI TCCNGGA 2 cut(s) 1510, 2070
PleI GAGTC 3 cut(s) 37, 1055, 3508
PmaCI CACGTG 1 cut(s) 2615
PmlI CACGTG 1 cut(s) 2615
PpsI GAGTC 3 cut(s) 37, 1055, 3508
Ppu21I YACGTR 2 cut(s) 2615, 3563
PpuMI RGGWCCY 1 cut(s) 3403
PscI ACATGT 1 cut(s) 620
PshBI ATTAAT 2 cut(s) 1352, 3825
PsiI TTATAA 1 cut(s) 191
Psp124BI GAGCTC 1 cut(s) 1007
Psp1406I AACGTT 2 cut(s) 186, 4024
Psp5II RGGWCCY 1 cut(s) 3403
Psp6I CCWGG 5 cut(s) 1510, 1650, 2070, 3016, 3229
PspCI CACGTG 1 cut(s) 2615
PspEI GGTNACC 1 cut(s) 1727
PspFI CCCAGC 1 cut(s) 1637
PspGI CCWGG 5 cut(s) 1510, 1650, 2070, 3016, 3229
PspN4I GGNNCC 9 cut(s) 232, 540, 854, 1119, 1188, 2124, 2787, 3789, 4034
PspPI GGNCC 7 cut(s) 22, 320, 445, 538, 2531, 3403, 4033
PspPPI RGGWCCY 1 cut(s) 3403
PspXI VCTCGAGB 1 cut(s) 2010
PstI CTGCAG 2 cut(s) 905, 1024
PstNI CAGNNNCTG 2 cut(s) 1046, 2609
PsuI RGATCY 7 cut(s) 178, 230, 1117, 2659, 2809, 2867, 3374
PvuII CAGCTG 3 cut(s) 1277, 1741, 3461
RsaI GTAC 7 cut(s) 350, 627, 839, 1296, 2124, 3006, 3608
RsaNI GTAC 7 cut(s) 349, 626, 838, 1295, 2123, 3005, 3607
RseI CAYNNNNRTG 3 cut(s) 1560, 3681, 3885
SacI GAGCTC 1 cut(s) 1007
Sau96I GGNCC 7 cut(s) 22, 320, 445, 538, 2531, 3403, 4033
SchI GAGTC 3 cut(s) 37, 1055, 3509
ScrFI CCNGG 6 cut(s) 1512, 1652, 1809, 2072, 3018, 3231
SduI GDGCHC 6 cut(s) 1007, 2836, 2970, 3589, 3780, 3939
SfcI CTRYAG 4 cut(s) 901, 1020, 1829, 2457
Sfr274I CTCGAG 3 cut(s) 2010, 2136, 2802
SinI GGWCC 3 cut(s) 320, 2531, 3403
SlaI CTCGAG 3 cut(s) 2010, 2136, 2802
SmiI ATTTAAAT 1 cut(s) 201
SmiMI CAYNNNNRTG 3 cut(s) 1560, 3681, 3885
SmlI CTYRAG 4 cut(s) 2010, 2136, 2802, 3610
SmoI CTYRAG 4 cut(s) 2010, 2136, 2802, 3610
SsiI CCGC 5 cut(s) 14, 2120, 2665, 2754, 3325
SspI AATATT 2 cut(s) 166, 2773
SstI GAGCTC 1 cut(s) 1007
StyD4I CCNGG 6 cut(s) 1510, 1650, 1807, 2070, 3016, 3229
StyI CCWWGG 4 cut(s) 887, 1524, 1612, 2336
SwaI ATTTAAAT 1 cut(s) 201
TaaI ACNGT 8 cut(s) 604, 704, 935, 2465, 2484, 3711, 3768, 4093
TaiI ACGT 5 cut(s) 189, 2425, 2617, 3565, 4027
TaqI TCGA 9 cut(s) 48, 97, 1291, 2011, 2137, 2803, 2938, 2997, 3820
TatI WGTACW 4 cut(s) 348, 837, 1294, 3606
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 8 cut(s) 332, 694, 1877, 2498, 3316, 3349, 3653, 3994
TseFI GTSAC 5 cut(s) 929, 1502, 1646, 1957, 3640
Tsp45I GTSAC 5 cut(s) 929, 1502, 1646, 1957, 3640
TspGWI ACGGA 1 cut(s) 1193
Van91I CCANNNNNTGG 2 cut(s) 2486, 4042
VneI GTGCAC 1 cut(s) 3776
VpaK11BI GGWCC 3 cut(s) 320, 2531, 3403
VspI ATTAAT 2 cut(s) 1352, 3825
XagI CCTNNNNNAGG 1 cut(s) 3620
XapI RAATTY 8 cut(s) 196, 1136, 1390, 1496, 2142, 2267, 3107, 3743
XbaI TCTAGA 2 cut(s) 2677, 3619
XceI RCATGY 4 cut(s) 64, 624, 2305, 3059
XhoI CTCGAG 3 cut(s) 2010, 2136, 2802
XmaJI CCTAGG 1 cut(s) 2336
XmiI GTMKAC 3 cut(s) 2460, 2594, 3040
XmnI GAANNNNTTC 4 cut(s) 1243, 2280, 2345, 3746
Zsp2I ATGCAT 1 cut(s) 3061
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.