MD16G1000900.v1.1

Serine threonine-protein kinase sepA-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006663
Physical Location & Seq
Forward (+)
49048 .. 59194
10147 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1000900.v1.1.491

Sequence Viewer

Length: 4260 bp
ATGTCTCGGCAAGCGGCGTCGGCCCACTTCCACAAATCCAAGACTCTCGATAACAAATACATGCTTGGAGATGAGATTGGAAAGGGAGCATATGGTCGAGTTTACAAGGGTTTGGATTTGGAGAATGGAGACTTTGTCGCAATTAAACAAGTTTCTTTGGAGAATATTGCTCAGGAGGATCTCAACATTATAATGCAAGAGATTGATTTACTCAAGAATTTAAATCACAAAAACATTGTGAAGTACCTTGGATCCTTAAAGACAAAGACTCATCTTCACATAATCCTGGAATATGTGGAGAATGGCTCACTTGCAAACATTATCAAGCCAAACAAATTTGGACCTTTCCCTGAATCATTGGTAGCTGTGTACATTGCTCAGGTTCTGGAGGGGTTGGTATATTTACATGAGCAGGGAGTAATCCATCGCGATATCAAGGGTGCAAATATATTGACAACTAAAGAGGGGCTTGTGAAACTTGCTGATTTTGGTGTTGCGACAAAACTGACTGAGGCGGATGTTAATACACATTCAGTTGTTGGAACGCCATACTGGATGGCCCCAGAGGTGATTGAAATGTCGGGAGTTTGTGCTGCTTCGGACATTTGGAGTGTTGGGTGCACTGTCATTGAACTTCTTACATGTGTACCTCCTTACTACGATCTACAGCCTATGCCAGCATTGTTTCGTATTGTTCAGGATGAGCATCCTCCCATACCTGACAGCCTATCACCGGACATTACTGATTTTCTGGGTCAGTGCTTTAAGAAGGATGCTAGGCACCGCCCTGATGCAAAGACATTGCTTTCTCACCCTTGGATACAAAACTGCCGGCGAGCTTTGCAATCTTCAATTCGTCATAGTGGAACTCTAAGACAAGATGCTTCAATAGGTGCAGAAATTTCCAATGGAGATAATCAGGGTTCTGCTGAAAGTCCTTCTGCAGAGAAAGTTGAAGTAGCTGCTTCAACCATTAAAGCAGACTCTGGGAAAGAATTATTATCAACTGAGGTTCCTGATATGGGAAGATCTGATGATAACCCTGCTTCAGATGTAAAATCGGTTGAGGAGAAAACGGATAATTTAGAAGATGATCTGACAGATGAAGTTCCCACATTAGCTATCCATGAAAAGTCATCTTTGCAAAATGGTTCTGGCAGAATATCTTCTCAGGAATTGGCTGCCTCTGAGCCAACTGAGCTCGACGAGCCACCACATGCTAGCAACCATGATGCAGTGCTGGTGAATGGTGAGGTCAGATCTCCTGAATTAACGACAAAAAATGTAAGTGGCAAACAAGGTGGAAAAGGTGTTGGTTATAGAGCTTTCGGTTTTGGAACAAGAAATCAGGATGGTAGTTTTCAAAAGGCTGCCAAGATGCCAGTTTTGTTGGGAGGTAATGAACTCAGTAAATTTAGTGATACTCCAGGAGATGCTTCCTTGGATGATCTGTTTCATCCATTGGATAAGCATCCAGAGGATAGGGCAACTGAGGCTTCGACATCTGCATCTATGTCACAGTCAAACCAAGGCAATACACCTGGGAACGATGCTGGGAAAAGCGACCTGGCTACAAAGTTGAGAGCTACAATTGCCCAAAAACAAATGGAGAGTGAATTGGGTCAGGCTAACGGCAGTGGTGGCAACCTGTTACAGCTTATGATGGGTGTTCTCAAAGATGATGTGATTGATATTGGTGGTTTGGTTTTCGACGAAAAGATGCCTGGAGAAAACCTATTTCCCCTACAGGCTGTTGAGTTCAGCCGGTTGGTTGGGTCATTGAGACCAGATGAAACAGAAGATGTTATTGTCTCTGCCTGTCAGAAACTCATTGTTATCTTCCATCAGCGCCCAGAGCAGAAAATTGTTTTTGTTACCCAGCATGGTTTGCTCCCTCTAATGGAATTGCTTGAAGTACCTAAAACTCGTGTTATATGTTCTGTGCTTCAAATTATAAATCGGATAATCAAAGATAATACTGATTTCCTGGAAAATGCTTGTCTTGTGGGTCTGATCCCAGTGATAATGAGCTTTGCCGTTCCTAATCATTTCCGGGAAATTCGTATGGAAGCAGCTTATTTCATACAGCAGCTTTGTCAATCAAGCCCTTCAACGTTGCAAATGTTCATAGCTTGCCGTGGAATACCTGTTTTGGTGGGCTTTTTAGAGGCTGACTATGCAAAATTCAGGGAAATGGTTCATCTAGCAATTGACGGGATGTGGCAGGTCTTTAAGCTTCAACAATCAACTCCTAGAAATGATTTTTGTCGCATAGCTGCGAAGAATGGAATACTGCTGAGGCTTATTAACACCCTTTATAGCTTGAATGAGGCAACCCGCCTAGCTTCCATATCTGTCGGGGGTGGATTTCCACTAGAAGGTTCAGCTCAGCGCCCACGGTCTGGTTCATTAGATTCTGGTCATCCGATTTTTGCTCAGAGTGACGTGCTGCTTTCTACAACTGATCAACATGATCTCTCCAAGGTTAGACATGGATTGATTGATTTTCATTTGTCAACTGGAACAGCAGAACCTGCACGTGCTTCAACTTCAAACTCTCAAAGATCAGATGCCAATCAATCAGATCCAAGATATCTTCATCTAGATACTGATAGAGCTCAATCTAGCAATGTGGTAGTGGAAGCTATAGTTCCTTCTAAATTGACAGACTCAACATCAGTAGACAAAGTAGTAAATATTACAACTAAGGAACCTTCCATTACCTCTCGGGATCTAGACCTCAGGCAGCAGCGACCTGCTAATTCTTCTAGTAGGGCATCCACAGATAGACCTCCAAAAATGATGGATGGTACATCAAATGGGTTTTCAACAACGGTAACTACTCAACAGGAGCAAGTTCGTCCCCTTCTTAGCTTGTTAGATAAAGAGCCTCCTTCCAGACACTTTTCTGGTCAGCTCGAGTTTGTACGCCACCTGCCAGGTTTGGAGAGACATGAAAGTATATTGCCCCTGTTACATGCTTCTAATGAAAAGAAGACAAATGGAGAATTAGATTTCCTGATGGCTGAATTTGCTGACGTCTCTCAACGTGGTAGAGAAAATGGGAATGTTGATTCCACTGCTAGAGTTTCTCATAAAACGATGAATAAAGAGATGGGAACAGTAGCATCTATCAAAGGAGCTGCTTCCACCTCTGGCATAGCATCTCAGACAGCATCAGGTGTACTTTCTGGTTCTGGAGTTTTAAATGCTAGACCAGGCAGTGCAACATCATCAGGTTTACTTTCCCACATGGTTTCAACATTGAATGCAGATGTTGCGAGGGAATACCTAGAAAAGGTGGCTGATCTTCTGCTTGAGTTTGCACAAGCAGATACTACTGTAAAGTCATACATGTGTAGCCAAAGTTTGCTCAGCCGTCTTTTCAAGATGTTCAATAGGGTGGAGCCCCCTATTCTTTTGAAGATACTGAAGTGTGTAAACCATTTGTCAACTGATCCAAACTGCTTAGAGAATCTTCAGCGGGCAGAAGCAATTAAATATTTGATTCCAAACCTTGAACTCAAAGAAGGAGCTCTTGTATCTCAAATACATCATGAGGTACTCAATGCCCTGTTCAATCTGTGCAAGATTAACAAGAGAAGACAGGAACAAGCAGCTGAGAATGGAATAATTCCACACTTAATGCACTTTATTGAGTCTAATTCTCCTTTGAAACAATGTGCGTTGCCTTTACTGTGTGACATGGCTCATGCATCACGAAATTCGAGGGAGCAACTAAGGGCTCATGGTGGGTTGGATGTTTACTTAAGCCTTCTCGAGGATGAACTTTGGTCTGTGACTGCATTGGATTCGATTGCCGTGTGCTTAGCTCATGACAATGACAACCGGAAGGTGGAACAAGCATTATTGAAAAAGGATGCAGTTCAGAAATTGGTGAAATTCTTCCAGTGCTGTCCAGAACAGTATTTCGTGCACATATTGGAGCCATTTTTGAAAATTATAACGAAATCATCTCGAATTAATACAACATTAGCTGTAAATGGTTTGACACCGCTGCTCATCACGAGACTGGACCATCAGGATGCAATTGCCCGTCTAAATCTTCTTAAACTGATAAAGGCTGTTTATGAGCACCACCCGCGGCCAAAGCAATTGATTGTGGAGAATGATCTGCCTCAGAAGCTTCAAAATCTAATAGAAGAACGCAGAGATGGGCAACGATCGGGTGGCCAAGTGTTGGTAAAACAAATGGCTACTTCGCTGCTAAAAGCACTACATATTAACACCGTCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000278 GO:0000281 GO:0000910 GO:0001558 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005813 GO:0005815 GO:0005829 GO:0005856 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007049 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0008361 GO:0009893 GO:0009987 GO:0010324 GO:0010562 GO:0010604 GO:0015630 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022402 GO:0022603 GO:0022604 GO:0023014 GO:0023052 GO:0030587 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032147 GO:0032268 GO:0032270 GO:0032502 GO:0032506 GO:0032535 GO:0033554 GO:0033674 GO:0035556 GO:0036089 GO:0036211 GO:0040008 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0045995 GO:0046777 GO:0048518 GO:0048522 GO:0048638 GO:0048856 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051246 GO:0051247 GO:0051301 GO:0051302 GO:0051338 GO:0051347 GO:0051510 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0061024 GO:0061387 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090066 GO:0090702 GO:0099024 GO:0099120 GO:0140096 GO:1901564 GO:1903047 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1420

Amino Acids

155.82

Weight (kDa)

5.98

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 21 - 274 5.9e-73 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 22 - 270 1.4e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 191, 1955, 3965
AarI CACCTGC 1 cut(s) 2943
AatII GACGTC 1 cut(s) 3042
Acc36I ACCTGC 4 cut(s) 2215, 2542, 2764, 2943
AccB1I GGYRCC 1 cut(s) 780
AccB7I CCANNNNNTGG 2 cut(s) 2402, 4201
AccI GTMKAC 1 cut(s) 2682
AccII CGCG 2 cut(s) 429, 4105
AciI CCGC 8 cut(s) 14, 515, 784, 2338, 3484, 4016, 4103, 4105
AclI AACGTT 1 cut(s) 2114
AclWI GGATC 7 cut(s) 186, 246, 259, 2008, 2579, 2739, 3452
AcoI YGGCCR 2 cut(s) 4106, 4192
AcsI RAATTY 9 cut(s) 217, 335, 900, 1412, 2058, 2183, 3029, 3724, 3902
AcuI CTGAAG 3 cut(s) 1032, 3452, 3464
AcvI CACGTG 1 cut(s) 2540
AcyI GRCGYC 2 cut(s) 17, 3039
AfaI GTAC 8 cut(s) 245, 371, 648, 1917, 2812, 2928, 3186, 3564
AfiI CCNNNNNNNGG 9 cut(s) 733, 1022, 1900, 2378, 2402, 3298, 3781, 3856, 4201
AflII CTTAAG 1 cut(s) 3769
AflIII ACRYGT 2 cut(s) 641, 3354
AjiI CACGTC 1 cut(s) 2446
AjnI CCWGG 8 cut(s) 285, 1426, 1540, 1566, 1723, 1986, 2938, 3217
Alw21I GWGCWC 6 cut(s) 623, 1203, 2620, 3538, 3939, 4098
Alw26I GTCTC 7 cut(s) 9, 123, 1777, 1816, 2944, 3046, 4024
Alw44I GTGCAC 2 cut(s) 619, 3935
AlwI GGATC 7 cut(s) 186, 246, 259, 2008, 2579, 2739, 3452
AlwNI CAGNNNCTG 3 cut(s) 385, 986, 2534
Ama87I CYCGRG 3 cut(s) 2727, 2918, 3779
AoxI GGCC 4 cut(s) 21, 558, 4106, 4192
ApaLI GTGCAC 2 cut(s) 619, 3935
ApoI RAATTY 9 cut(s) 217, 335, 900, 1412, 2058, 2183, 3029, 3724, 3902
AseI ATTAAT 1 cut(s) 3984
Asp700I GAANNNNTTC 3 cut(s) 1165, 2196, 3905
AspLEI GCGC 2 cut(s) 1851, 2394
AspS9I GGNCC 4 cut(s) 22, 341, 559, 4036
AsuC2I CCSGG 1 cut(s) 2054
AsuHPI GGTGA 6 cut(s) 580, 723, 803, 1255, 1262, 3910
AsuNHI GCTAGC 1 cut(s) 1220
AvaI CYCGRG 3 cut(s) 2727, 2918, 3779
AvaII GGWCC 2 cut(s) 341, 4036
AxyI CCTNAGG 1 cut(s) 2741
BaeGI GKGCMC 2 cut(s) 623, 3939
BaeI ACNNNNGTAYC 6 cut(s) 630, 663, 3327, 3360, 3419, 3452
BalI TGGCCA 1 cut(s) 4194
BamHI GGATCC 1 cut(s) 251
BanI GGYRCC 1 cut(s) 780
BanII GRGCYC 5 cut(s) 1203, 2620, 3411, 3538, 3748
BauI CACGAG 2 cut(s) 1926, 4027
BbrPI CACGTG 1 cut(s) 2540
BbsI GAAGAC 2 cut(s) 3002, 3610
Bbv12I GWGCWC 6 cut(s) 623, 1203, 2620, 3538, 3939, 4098
BbvCI CCTCAGC 1 cut(s) 2297
BceAI ACGGC 5 cut(s) 1648, 2021, 2121, 3363, 3806
BcgI CGANNNNNNTGC 2 cut(s) 3795, 3829
BciT130I CCWGG 8 cut(s) 287, 1428, 1542, 1568, 1725, 1988, 2940, 3219
BciVI GTATCC 1 cut(s) 813
BclI TGATCA 1 cut(s) 2464
BcnI CCSGG 1 cut(s) 2054
BcoDI GTCTC 7 cut(s) 9, 123, 1777, 1816, 2944, 3046, 4024
BfmI CTRYAG 4 cut(s) 665, 942, 1745, 2646
BfoI RGCGCY 2 cut(s) 1852, 2395
BfrI CTTAAG 1 cut(s) 3769
BfuAI ACCTGC 4 cut(s) 2215, 2542, 2764, 2943
BfuI GTATCC 1 cut(s) 813
BglII AGATCT 2 cut(s) 1028, 1259
BlpI GCTNAGC 3 cut(s) 2388, 3374, 3829
Bme1390I CCNGG 9 cut(s) 287, 1428, 1542, 1568, 1725, 1988, 2054, 2940, 3219
Bme18I GGWCC 2 cut(s) 341, 4036
BmeT110I CYCGRG 3 cut(s) 2727, 2918, 3779
BmgBI CACGTC 1 cut(s) 2446
BmgT120I GGNCC 4 cut(s) 22, 341, 559, 4036
BmiI GGNNCC 7 cut(s) 253, 561, 782, 1014, 2712, 3408, 3948
BmrFI CCNGG 9 cut(s) 287, 1428, 1542, 1568, 1725, 1988, 2054, 2940, 3219
BmrI ACTGGG 1 cut(s) 2012
BmtI GCTAGC 1 cut(s) 1224
BmuI ACTGGG 1 cut(s) 2012
BpiI GAAGAC 2 cut(s) 3002, 3610
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 4 cut(s) 407, 1410, 1746, 3219
Bpu10I CCTNAGC 3 cut(s) 171, 378, 2297
Bpu1102I GCTNAGC 3 cut(s) 2388, 3374, 3829
BpuEI CTTGAG 2 cut(s) 197, 3338
BpuMI CCSGG 1 cut(s) 2054
BsaAI YACGTR 1 cut(s) 2540
BsaBI GATNNNNATC 3 cut(s) 705, 1470, 2574
BsaHI GRCGYC 2 cut(s) 17, 3039
BsaI GGTCTC 1 cut(s) 1777
BsaJI CCNNGG 9 cut(s) 247, 815, 1440, 1528, 1541, 2137, 2396, 2481, 4103
BsaWI WCCGGW 2 cut(s) 733, 3849
BsaXI ACNNNNNCTCC 4 cut(s) 120, 150, 3525, 3555
Bsc4I CCNNNNNNNGG 9 cut(s) 733, 1022, 1900, 2378, 2402, 3298, 3781, 3856, 4201
Bse118I RCCGGY 2 cut(s) 831, 1764
Bse1I ACTGG 6 cut(s) 557, 1382, 2018, 2524, 3910, 4038
Bse21I CCTNAGG 1 cut(s) 2741
Bse3DI GCAATG 3 cut(s) 372, 800, 2635
Bse8I GATNNNNATC 3 cut(s) 705, 1470, 2574
BseBI CCWGG 8 cut(s) 287, 1428, 1542, 1568, 1725, 1988, 2940, 3219
BseDI CCNNGG 9 cut(s) 247, 815, 1440, 1528, 1541, 2137, 2396, 2481, 4103
BseJI GATNNNNATC 3 cut(s) 705, 1470, 2574
BseLI CCNNNNNNNGG 9 cut(s) 733, 1022, 1900, 2378, 2402, 3298, 3781, 3856, 4201
BseMI GCAATG 3 cut(s) 372, 800, 2635
BseNI ACTGG 6 cut(s) 557, 1382, 2018, 2524, 3910, 4038
BseRI GAGGAG 1 cut(s) 1082
BseSI GKGCMC 2 cut(s) 623, 3939
BseYI CCCAGC 2 cut(s) 1553, 1878
BsgI GTGCAG 2 cut(s) 915, 2520
Bsh1236I CGCG 2 cut(s) 429, 4105
Bsh1285I CGRYCG 1 cut(s) 4187
BshFI GGCC 4 cut(s) 23, 560, 4108, 4194
BshNI GGYRCC 1 cut(s) 780
BsiEI CGRYCG 1 cut(s) 4187
BsiHKAI GWGCWC 6 cut(s) 623, 1203, 2620, 3538, 3939, 4098
BsiHKCI CYCGRG 3 cut(s) 2727, 2918, 3779
BsiSI CCGG 5 cut(s) 734, 832, 1765, 2053, 3850
BslFI GGGAC 1 cut(s) 2847
BslI CCNNNNNNNGG 9 cut(s) 733, 1022, 1900, 2378, 2402, 3298, 3781, 3856, 4201
BsmAI GTCTC 7 cut(s) 9, 123, 1777, 1816, 2944, 3046, 4024
BsmBI CGTCTC 1 cut(s) 3046
BsmFI GGGAC 1 cut(s) 2847
BsmI GAATGC 1 cut(s) 3274
BsnI GGCC 4 cut(s) 23, 560, 4108, 4194
Bso31I GGTCTC 1 cut(s) 1777
BsoBI CYCGRG 3 cut(s) 2727, 2918, 3779
Bsp1286I GDGCHC 8 cut(s) 623, 1203, 2620, 3411, 3538, 3748, 3939, 4098
Bsp1407I TGTACA 1 cut(s) 369
Bsp1720I GCTNAGC 3 cut(s) 2388, 3374, 3829
Bsp68I TCGCGA 1 cut(s) 429
BspACI CCGC 8 cut(s) 14, 515, 784, 2338, 3484, 4016, 4103, 4105
BspANI GGCC 4 cut(s) 23, 560, 4108, 4194
BspFNI CGCG 2 cut(s) 429, 4105
BspHI TCATGA 2 cut(s) 3556, 3835
BspLI GGNNCC 7 cut(s) 253, 561, 782, 1014, 2712, 3408, 3948
BspMAI CTGCAG 1 cut(s) 946
BspMI ACCTGC 4 cut(s) 2215, 2542, 2764, 2943
BspOI GCTAGC 1 cut(s) 1224
BspPI GGATC 7 cut(s) 186, 246, 259, 2008, 2579, 2739, 3452
BspT107I GGYRCC 1 cut(s) 780
BspTI CTTAAG 1 cut(s) 3769
BspTNI GGTCTC 1 cut(s) 1777
BsrDI GCAATG 3 cut(s) 372, 800, 2635
BsrFI RCCGGY 2 cut(s) 831, 1764
BsrGI TGTACA 1 cut(s) 369
BsrI ACTGG 6 cut(s) 557, 1382, 2018, 2524, 3910, 4038
BssAI RCCGGY 2 cut(s) 831, 1764
BssECI CCNNGG 9 cut(s) 247, 815, 1440, 1528, 1541, 2137, 2396, 2481, 4103
BssNI GRCGYC 2 cut(s) 17, 3039
BssSI CACGAG 2 cut(s) 1926, 4027
BssT1I CCWWGG 5 cut(s) 247, 815, 1440, 1528, 2481
Bst2BI CACGAG 2 cut(s) 1926, 4027
Bst2UI CCWGG 8 cut(s) 287, 1428, 1542, 1568, 1725, 1988, 2940, 3219
Bst4CI ACNGT 9 cut(s) 625, 1521, 2400, 2836, 3124, 3343, 3699, 3927, 4252
BstACI GRCGYC 2 cut(s) 17, 3039
BstAFI CTTAAG 1 cut(s) 3769
BstAPI GCANNNNNTGC 3 cut(s) 1888, 2534, 3278
BstAUI TGTACA 1 cut(s) 369
BstBAI YACGTR 1 cut(s) 2540
BstC8I GCNNGC 7 cut(s) 12, 678, 833, 837, 1222, 2134, 3486
BstDSI CCRYGG 3 cut(s) 2137, 2396, 4103
BstENI CCTNNNNNAGG 2 cut(s) 3296, 3779
BstFNI CGCG 2 cut(s) 429, 4105
BstH2I RGCGCY 2 cut(s) 1852, 2395
BstHHI GCGC 2 cut(s) 1851, 2394
BstMAI GTCTC 7 cut(s) 9, 123, 1777, 1816, 2944, 3046, 4024
BstMCI CGRYCG 1 cut(s) 4187
BstNI CCWGG 8 cut(s) 287, 1428, 1542, 1568, 1725, 1988, 2940, 3219
BstNSI RCATGY 5 cut(s) 64, 645, 1220, 2981, 3358
BstSCI CCNGG 9 cut(s) 285, 1426, 1540, 1566, 1723, 1986, 2052, 2938, 3217
BstSFI CTRYAG 4 cut(s) 665, 942, 1745, 2646
BstSLI GKGCMC 2 cut(s) 623, 3939
BstUI CGCG 2 cut(s) 429, 4105
BstV2I GAAGAC 2 cut(s) 3002, 3610
BstX2I RGATCY 6 cut(s) 178, 251, 1028, 1259, 2584, 2731
BstYI RGATCY 6 cut(s) 178, 251, 1028, 1259, 2584, 2731
Bsu36I CCTNAGG 1 cut(s) 2741
BsuI GTATCC 1 cut(s) 813
BsuRI GGCC 4 cut(s) 23, 560, 4108, 4194
BtgI CCRYGG 3 cut(s) 2137, 2396, 4103
BtgZI GCGATG 1 cut(s) 410
BtrI CACGTC 1 cut(s) 2446
BtsI GCAGTG 4 cut(s) 1242, 1642, 3078, 3229
BtsIMutI CAGTG 8 cut(s) 621, 764, 1242, 1642, 2025, 3078, 3229, 3917
BtuMI TCGCGA 1 cut(s) 429
BveI ACCTGC 4 cut(s) 2215, 2542, 2764, 2943
Cac8I GCNNGC 7 cut(s) 12, 678, 833, 837, 1222, 2134, 3486
CaiI CAGNNNCTG 3 cut(s) 385, 986, 2534
CciI TCATGA 2 cut(s) 3556, 3835
CfoI GCGC 2 cut(s) 1851, 2394
Cfr10I RCCGGY 2 cut(s) 831, 1764
Cfr13I GGNCC 4 cut(s) 22, 341, 559, 4036
Cfr42I CCGCGG 1 cut(s) 4106
CseI GACGC 1 cut(s) 6
Csp6I GTAC 8 cut(s) 244, 370, 647, 1916, 2811, 2927, 3185, 3563
CspCI CAANNNNNGTGG 2 cut(s) 1282, 1317
CviQI GTAC 8 cut(s) 244, 370, 647, 1916, 2811, 2927, 3185, 3563
DraI TTTAAA 2 cut(s) 222, 3207
EaeI YGGCCR 2 cut(s) 4106, 4192
EciI GGCGGA 1 cut(s) 530
Ecl136II GAGCTC 3 cut(s) 1201, 2618, 3536
Eco130I CCWWGG 5 cut(s) 247, 815, 1440, 1528, 2481
Eco24I GRGCYC 5 cut(s) 1203, 2620, 3411, 3538, 3748
Eco31I GGTCTC 1 cut(s) 1777
Eco32I GATATC 2 cut(s) 433, 2594
Eco47I GGWCC 2 cut(s) 341, 4036
Eco53kI GAGCTC 3 cut(s) 1201, 2618, 3536
Eco57I CTGAAG 3 cut(s) 1032, 3452, 3464
Eco72I CACGTG 1 cut(s) 2540
Eco81I CCTNAGG 1 cut(s) 2741
Eco88I CYCGRG 3 cut(s) 2727, 2918, 3779
EcoICRI GAGCTC 3 cut(s) 1201, 2618, 3536
EcoNI CCTNNNNNAGG 2 cut(s) 3296, 3779
EcoRII CCWGG 8 cut(s) 285, 1426, 1540, 1566, 1723, 1986, 2938, 3217
EcoRV GATATC 2 cut(s) 433, 2594
EcoT14I CCWWGG 5 cut(s) 247, 815, 1440, 1528, 2481
EcoT22I ATGCAT 1 cut(s) 3718
EcoT38I GRGCYC 5 cut(s) 1203, 2620, 3411, 3538, 3748
ErhI CCWWGG 5 cut(s) 247, 815, 1440, 1528, 2481
Esp3I CGTCTC 1 cut(s) 3046
FalI AAGNNNNNCTT 4 cut(s) 453, 485, 3522, 3554
FaqI GGGAC 1 cut(s) 2847
FauI CCCGC 3 cut(s) 2345, 3477, 4110
FauNDI CATATG 1 cut(s) 91
FbaI TGATCA 1 cut(s) 2464
FblI GTMKAC 1 cut(s) 2682
FriOI GRGCYC 5 cut(s) 1203, 2620, 3411, 3538, 3748
GlaI GCGC 2 cut(s) 1850, 2393
GsaI CCCAGC 2 cut(s) 1557, 1882
GsuI CTGGAG 4 cut(s) 407, 1410, 1746, 3219
HaeII RGCGCY 2 cut(s) 1852, 2395
HaeIII GGCC 4 cut(s) 23, 560, 4108, 4194
HapII CCGG 5 cut(s) 734, 832, 1765, 2053, 3850
HgaI GACGC 1 cut(s) 6
HhaI GCGC 2 cut(s) 1851, 2394
Hin1I GRCGYC 2 cut(s) 17, 3039
Hin6I GCGC 2 cut(s) 1849, 2392
HinP1I GCGC 2 cut(s) 1849, 2392
HincII GTYRAC 2 cut(s) 2517, 3453
HindII GTYRAC 2 cut(s) 2517, 3453
HindIII AAGCTT 2 cut(s) 2234, 4145
HpaII CCGG 5 cut(s) 734, 832, 1765, 2053, 3850
HphI GGTGA 6 cut(s) 580, 723, 803, 1255, 1262, 3910
Hpy99I CGWCG 3 cut(s) 22, 1208, 1715
HpyCH4III ACNGT 9 cut(s) 625, 1521, 2400, 2836, 3124, 3343, 3699, 3927, 4252
HpyCH4IV ACGT 5 cut(s) 2114, 2445, 2539, 3039, 3049
HpySE526I ACGT 5 cut(s) 2114, 2445, 2539, 3039, 3049
Hsp92I GRCGYC 2 cut(s) 17, 3039
HspAI GCGC 2 cut(s) 1849, 2392
KroI GCCGGC 1 cut(s) 831
KroNI GCCGGC 1 cut(s) 833
Ksp22I TGATCA 1 cut(s) 2464
KspI CCGCGG 1 cut(s) 4106
LmnI GCTCC 8 cut(s) 86, 1896, 2851, 3140, 3406, 3533, 3733, 3946
MaeII ACGT 5 cut(s) 2114, 2445, 2539, 3039, 3049
MaeIII GTNAC 8 cut(s) 1515, 1650, 1873, 2441, 2836, 2973, 3701, 3799
MfeI CAATTG 4 cut(s) 1590, 2208, 4050, 4115
MflI RGATCY 6 cut(s) 178, 251, 1028, 1259, 2584, 2731
MhlI GDGCHC 8 cut(s) 623, 1203, 2620, 3411, 3538, 3748, 3939, 4098
MlsI TGGCCA 1 cut(s) 4194
MluNI TGGCCA 1 cut(s) 4194
MlyI GAGTC 5 cut(s) 37, 262, 977, 2663, 3668
MmeI TCCRAC 2 cut(s) 520, 3738
Mox20I TGGCCA 1 cut(s) 4194
Mph1103I ATGCAT 1 cut(s) 3718
MroNI GCCGGC 1 cut(s) 831
MroXI GAANNNNTTC 3 cut(s) 1165, 2196, 3905
MscI TGGCCA 1 cut(s) 4194
MslI CAYNNNNRTG 4 cut(s) 191, 3355, 3840, 4044
Msp20I TGGCCA 1 cut(s) 4194
MspA1I CMGCKG 4 cut(s) 3484, 3620, 4018, 4105
MspCI CTTAAG 1 cut(s) 3769
MspI CCGG 5 cut(s) 734, 832, 1765, 2053, 3850
MspR9I CCNGG 9 cut(s) 287, 1428, 1542, 1568, 1725, 1988, 2054, 2940, 3219
MunI CAATTG 4 cut(s) 1590, 2208, 4050, 4115
Mva1269I GAATGC 1 cut(s) 3274
MvaI CCWGG 8 cut(s) 287, 1428, 1542, 1568, 1725, 1988, 2940, 3219
MvnI CGCG 2 cut(s) 429, 4105
NaeI GCCGGC 1 cut(s) 833
NciI CCSGG 1 cut(s) 2054
NdeI CATATG 1 cut(s) 91
NgoMIV GCCGGC 1 cut(s) 831
NheI GCTAGC 1 cut(s) 1220
NlaIV GGNNCC 7 cut(s) 253, 561, 782, 1014, 2712, 3408, 3948
NmuCI GTSAC 4 cut(s) 1515, 2441, 3701, 3799
NruI TCGCGA 1 cut(s) 429
NsiI ATGCAT 1 cut(s) 3718
NspI RCATGY 5 cut(s) 64, 645, 1220, 2981, 3358
PaeR7I CTCGAG 2 cut(s) 2918, 3779
PagI TCATGA 2 cut(s) 3556, 3835
PaqCI CACCTGC 1 cut(s) 2943
PciI ACATGT 2 cut(s) 641, 3354
PctI GAATGC 1 cut(s) 3274
PdiI GCCGGC 1 cut(s) 833
PdmI GAANNNNTTC 3 cut(s) 1165, 2196, 3905
PfeI GAWTC 6 cut(s) 353, 2414, 3074, 3475, 3508, 3812
PflFI GACNNNGTC 1 cut(s) 134
PflMI CCANNNNNTGG 2 cut(s) 2402, 4201
PfoI TCCNGGA 4 cut(s) 285, 1426, 1986, 2052
Ple19I CGATCG 1 cut(s) 4187
PleI GAGTC 5 cut(s) 37, 262, 977, 2663, 3667
PmaCI CACGTG 1 cut(s) 2540
PmlI CACGTG 1 cut(s) 2540
PpsI GAGTC 5 cut(s) 37, 262, 977, 2663, 3667
Ppu21I YACGTR 1 cut(s) 2540
PscI ACATGT 2 cut(s) 641, 3354
PshBI ATTAAT 1 cut(s) 3984
PsiI TTATAA 3 cut(s) 191, 1955, 3965
Psp124BI GAGCTC 3 cut(s) 1203, 2620, 3538
Psp1406I AACGTT 1 cut(s) 2114
Psp6I CCWGG 8 cut(s) 285, 1426, 1540, 1566, 1723, 1986, 2938, 3217
PspCI CACGTG 1 cut(s) 2540
PspFI CCCAGC 2 cut(s) 1553, 1878
PspGI CCWGG 8 cut(s) 285, 1426, 1540, 1566, 1723, 1986, 2938, 3217
PspN4I GGNNCC 7 cut(s) 253, 561, 782, 1014, 2712, 3408, 3948
PspPI GGNCC 4 cut(s) 22, 341, 559, 4036
PspXI VCTCGAGB 1 cut(s) 2918
PstI CTGCAG 1 cut(s) 946
PstNI CAGNNNCTG 3 cut(s) 385, 986, 2534
PsuI RGATCY 6 cut(s) 178, 251, 1028, 1259, 2584, 2731
PsyI GACNNNGTC 1 cut(s) 134
PvuI CGATCG 1 cut(s) 4187
PvuII CAGCTG 1 cut(s) 3620
RruI TCGCGA 1 cut(s) 429
RsaI GTAC 8 cut(s) 245, 371, 648, 1917, 2812, 2928, 3186, 3564
RsaNI GTAC 8 cut(s) 244, 370, 647, 1916, 2811, 2927, 3185, 3563
RseI CAYNNNNRTG 4 cut(s) 191, 3355, 3840, 4044
SacI GAGCTC 3 cut(s) 1203, 2620, 3538
SacII CCGCGG 1 cut(s) 4106
Sau96I GGNCC 4 cut(s) 22, 341, 559, 4036
SchI GAGTC 5 cut(s) 37, 262, 977, 2663, 3668
ScrFI CCNGG 9 cut(s) 287, 1428, 1542, 1568, 1725, 1988, 2054, 2940, 3219
SduI GDGCHC 8 cut(s) 623, 1203, 2620, 3411, 3538, 3748, 3939, 4098
SfcI CTRYAG 4 cut(s) 665, 942, 1745, 2646
Sfr274I CTCGAG 2 cut(s) 2918, 3779
Sfr303I CCGCGG 1 cut(s) 4106
SgrBI CCGCGG 1 cut(s) 4106
SinI GGWCC 2 cut(s) 341, 4036
SlaI CTCGAG 2 cut(s) 2918, 3779
SmiI ATTTAAAT 1 cut(s) 222
SmiMI CAYNNNNRTG 4 cut(s) 191, 3355, 3840, 4044
SmlI CTYRAG 5 cut(s) 212, 2918, 3317, 3769, 3779
SmoI CTYRAG 5 cut(s) 212, 2918, 3317, 3769, 3779
SsiI CCGC 8 cut(s) 14, 515, 784, 2338, 3484, 4016, 4103, 4105
SspI AATATT 3 cut(s) 166, 2698, 3503
SstI GAGCTC 3 cut(s) 1203, 2620, 3538
StyD4I CCNGG 9 cut(s) 285, 1426, 1540, 1566, 1723, 1986, 2052, 2938, 3217
StyI CCWWGG 5 cut(s) 247, 815, 1440, 1528, 2481
SwaI ATTTAAAT 1 cut(s) 222
TaaI ACNGT 9 cut(s) 625, 1521, 2400, 2836, 3124, 3343, 3699, 3927, 4252
TaiI ACGT 5 cut(s) 2117, 2448, 2542, 3042, 3052
TatI WGTACW 2 cut(s) 369, 3184
TauI GCSGC 2 cut(s) 17, 4108
TfiI GAWTC 6 cut(s) 353, 2414, 3074, 3475, 3508, 3812
TscAI CASTG 8 cut(s) 628, 764, 1242, 1642, 2025, 3085, 3229, 3917
TseFI GTSAC 4 cut(s) 1515, 2441, 3701, 3799
Tsp45I GTSAC 4 cut(s) 1515, 2441, 3701, 3799
TspGWI ACGGA 1 cut(s) 1091
TspRI CASTG 8 cut(s) 628, 764, 1242, 1642, 2025, 3085, 3229, 3917
Tth111I GACNNNGTC 1 cut(s) 134
Van91I CCANNNNNTGG 2 cut(s) 2402, 4201
Vha464I CTTAAG 1 cut(s) 3769
VneI GTGCAC 2 cut(s) 619, 3935
VpaK11BI GGWCC 2 cut(s) 341, 4036
VspI ATTAAT 1 cut(s) 3984
XagI CCTNNNNNAGG 2 cut(s) 3296, 3779
XapI RAATTY 9 cut(s) 217, 335, 900, 1412, 2058, 2183, 3029, 3724, 3902
XbaI TCTAGA 2 cut(s) 2602, 2734
XceI RCATGY 5 cut(s) 64, 645, 1220, 2981, 3358
XhoI CTCGAG 2 cut(s) 2918, 3779
XmiI GTMKAC 1 cut(s) 2682
XmnI GAANNNNTTC 3 cut(s) 1165, 2196, 3905
ZraI GACGTC 1 cut(s) 3040
Zsp2I ATGCAT 1 cut(s) 3718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.