MD13G1006900.v1.1

Serine threonine-protein kinase sepA-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0006663
Physical Location & Seq
Reverse (-)
430932 .. 446978
16047 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1006900.v1.1.491

Sequence Viewer

Length: 4260 bp
ATGTCTCGGCAAGCGGCGTCGCCTCACTTCCACAAATCCAAGACGCTCGATAACAAATACATGCTTGGAGATGAGATTGGAAAGGGAGCATACGGTCGAGTTTACAAGGGTTTGGATTTGGAGAATGGGGACTTTGTCGCTATTAAACAAGTTTCTTTGGAGAATATTGCCCAGGAGGATCTCAACGTTATAATGCAAGAGATTGATTTACTCAAGAATTTAAATCACAAAAACATTGTGAAGTACCTTGGATCCTTAAAGACAAAGACTCATCTTCACATAATCCTAGAATATGTGGAGAATGGCTCACTTGCAAACATTATCAAGCCAAACAAATTTGGACCTTTCCCAGAATCATTGGTAGCTGTGTACATTGCTCAGGTTCTGGAGGGGTTAGTATATTTACATGAACAGGGCGTAATCCATCGGGATATCAAGGGTGCAAATATATTGACAACTAAAGAGGGACTTGTGAAACTAGCTGATTTCGGTGTTGCGACAAAACTGACTGAGGCGGATGCTAATACACATTCAGTTGTTGGAACACCATATTGGATGGCCCCAGAGGTGATTGAAATGTCGGGGGTTTGTGCTGCTTCAGACATTTGGAGTGTTGGGTGCACTGTTATTGAACTTCTTACATGCGTACCTCCTTACTATGATCTACAGCCTATGCCAGCACTGTTTCGTATTGTTCAGGATGAGCGTCCTCCCATACCTGACAGCCTATCTCTGGACATTACTGATTTTCTGGGTCAGTGCTTTAAGAAGGATGCTAGGCATCGGCCAGATGCAAAGACATTGCTTTCTCACCCTTGGATACAAAACTGCCGGCGAGCATTGCAATCTTCAATTCGTCATAGTGGAACTCTAAGAAAAGATGCTTCAATAGATGCAGAAATTTCCAATGGAGATAATCAGGGCTCTAGTGGACGTCCTTCTGCAGAGAAAGTTGAAGCAGCTGCTTCGACCATTAAAGCAGACTCTAGGAAAGAATTATTATCAACTGAGGTTTCTGATATGGGAAAATCTGATGATGACACTGCTTCAGATGTAAAATCTGTTGAGGAGAAGACGGATAATTTAGAAGATGATCTGACAGATCAAGTTCCCACATTAGCTATCCATGAAAAGTCATCTTTGCAAAATGGTTCTGGCATAATATCTTTTAAGGAATTGGCTGCCTCTGAGCCAACTGAGCTTGACGAGCCACCACATACTAGCAACCATGATGCAGTGCTGGTGAATGGTGAGGTCAGATCTCCTGAATTAATGACTAAAAATATAAGTGCCAAACAAGGAGGAAAAGGTGTTGGTTATAGAGCATTCGGTTTCGGAACAAGAAATCAGGATAGTAGTTTTCAAAAGGCTGCCAAGATGCCAGTTTCTTTGGGAGGTAATGAACTCAGTAAATTTAGTGATACTCCGGGAGATGCTTCCTTGGATGATCTGTTTCATCCATTGGATAAGCATCCAGAGGATAGGGCAACTGAGGCTTCGACATCTGCATCTATGTCACATTCAAACCAAGGGAATACACCTGGGAATGATGCTGGGAAAGGCGACCTGGCTACAAAGTTGAGAGCTACAATTGCTCAAAAACAAATGGAGAGTGAAATGGGTCAGGCTAATGGCAGTGGTGGCAACCTGTTACAGCTTATGATGGGTGTTCTCAAAGATGATGTGATTGATATTGGTGGTTTGGTTTTCGACGAAAAGATGCCTGGAGAAAACCTATTTCCCCTACAGGCTGTTGAGTTCAGCCGGTTGGTTGGGTCATTGAGACCAGATGAAACAGAAGATGTTATTGTCTCTGCCTGTCAGAAACTCATTGCTATCTTCCATCAGCGCCCAGAGCAGAAAATTGTTTTTGTTACCCAGCATGGTTTGATCCCTCTAGTGGAATTGCTTGAAGTACCTAAAACTCGAGTTATATGTTCTGTGCTTCAAATTATAAATCAGATAATCAAAGATAATACTGATTTCCAAGAAAATGCTTGTCTCGTGGGTTTGATCCCAGTGATAATGAGCTTTGCGGTTCCTAATCATTCCCGGGAAATTCGTATGGAAGCAGCTTATTTCTTACAGCAACTTTGTCAGTCAAGCGCCTTGACATTGCAAATGTTCATAGCTTGCCGTGGAATACCTGTTTTGGTGGGCTTTTTAGAGGCTGACTATGCAAAATTCAGGGAAATGGTTCATCTAGCAATTGATGGTATGTGGCAAGTCTTTAAGCTTCAGCGGTCAACTCCTAGAAATGATTTTTGTCGCATAGCCGCAAAGAATGGAATACTCCTGAGGCTCATTAACACCCTTCATAGCTTGAATGAGGCAACCCGCCTAGCTTCCATATCTGCCGGGGGTGGATTTCCACTAGAAGGTTCAGCTCAGCGCCCACGGTCTGGTTCATTAGATTCTGGTCATCCGATTTTTTCTCAGAGTGACACACCGCTTCCTACAACTGATCAACATGATCCCTCCAAGGTTAGACATGGAATGATTGATTTTCATTTGTCAACTGGAACAGCAGAACCTGCATGGGCTTCAACTTCAAACTCTCAAAGATCAGATGCCGATCAATCAGATCCAAGATATCTTCATCTAGATACTGATAGAGCTCAATCTAGCAATGTGGTAGTGGAAGCTTCAGTTCCTTCTAAATTGACAGACTCATCATCAGTAGACAAAGTAGTAAATATTACAACCAAGGAACCTTCAACTAGCTCTCGAGATCTAGACCTCAGGCAGCAGCGACCTGCCAATTCTTCTAGTAGGGCATCCACAGATAGACCTCCAAAAATGATGGAAGGTACATCAAATGGGTTTTCAACAACAGTAACTACTCAACAGGAGCAAGTTCGTCCCCTTCTTAGCTTGTTAGATAAAGAGCCTCCTTCCAGACGCTTTTCTGGTCAGCTCGAGTATGTACGCCACCTGCCAGGTTTGGAGAGACATGAAAGTATATGGCCCCTTTTACATGCTTCTAATGAAAAGAAGACAAATGGAGAATTAGATTTCCTGATGGCCGAATTTGCTGACGTCTCTCAACGTGGTAGAGAAAATGGGAATGTTGATTCCACTGCTAGAATTTCTCATAAAACGATAAATAAAGAGATGGGAACACTAGCATCTATCAAAGGAGCTGCTTCCACCTCTGGCATAGCATCTCAGACAGCATCTGGAGTACTTTCTGGTTCTGGAGTTCTAAATGCTAGACCAGGCAGTGCAACATCATCAGGTTTACTTTCCCACATGGTTTCAACATTGAATGCAGATGTTGCGAGGGAATACCTAGAAAAGGTGGCTGATCTTCTGCTTGAGTTTGCACAGGCAGATACTACTGTAAAGTCATACATGTGTAGCCAAAGTTTGCTCAGCCGTCTCTTCAAGATGTTCAATAGGGTGGAGCCCCCTATTCTTTTGAAGATACTGAAGTGTGTAAACCATTTGTCAACTGATCCAAACTGCTTAGAGAATCTTCAGCGGGCAGAAGCAATTAAATATTTGATTCCAAATCTGGAACTTAAAGAAGGAGCTCTTGTATCTCAAATACATCATGAGGTACTCAATGCCCTGTTCAATCTGTGTAAGATTAACAAGAGAAGACAGGAACAAGCAGCTGAGATTGGAATAATTCCACACTTAATGCACTTTATTGAGTCTAATTCTCCTTTGAAACAATATGCGTTGCCTTTACTGTGTGATATGGCTCATGCATCACGTAATTCGAGGGAGCAACTAAGGGCTCATGGTGGGTTGGATGTTTACCTAAGCCTTCTTGAGGATGAACTTTGGTCTGTGACTGCATTGGATTCGATTGCTGTGTGCTTGGCTCATGACAATGACAACCGGAAGGTGGAACAAGCATTATTGAAAAAGGATGCAGTTCAGAAATTGGTGAAATTCTTCCAGTGCTGTCCAGAACAGTATTTCGTGCACATATTGGAGCCTTTTTTGAAAATTATCACGAAATCATCTCGAATTAATACAACGTTAGCTGTTAATGGTTTGACACCGCTGCTTATCACAAGACTCGACCATCAGGATGCTATTGCCCGTCTAAATCTTCTTAAACTGATAAAGGCTGTTTATGAGCACCACCCGCGGCCAAAGCAATTGATTGTGGAGAATGATCTGCCTCAGAAGCTTCAAAATCTAATAGAAGAACGCAGAGATGGGCAAAGATTGGGTGGCCAAGTGTTGGTAAAACAAATGGCTACTTCGCTGCTTAAGGCACTACATATTAACACCGTCCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000278 GO:0000281 GO:0000910 GO:0001558 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005813 GO:0005815 GO:0005829 GO:0005856 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007049 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0008361 GO:0009893 GO:0009987 GO:0010324 GO:0010562 GO:0010604 GO:0015630 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022402 GO:0022603 GO:0022604 GO:0023014 GO:0023052 GO:0030587 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032147 GO:0032268 GO:0032270 GO:0032502 GO:0032506 GO:0032535 GO:0033554 GO:0033674 GO:0035556 GO:0036089 GO:0036211 GO:0040008 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0045995 GO:0046777 GO:0048518 GO:0048522 GO:0048638 GO:0048856 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051246 GO:0051247 GO:0051301 GO:0051302 GO:0051338 GO:0051347 GO:0051510 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0061024 GO:0061387 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090066 GO:0090702 GO:0099024 GO:0099120 GO:0140096 GO:1901564 GO:1903047 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1420

Amino Acids

156.09

Weight (kDa)

6.07

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 21 - 274 1.9e-72 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 22 - 270 7.2e-51 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 191, 1955
AarI CACCTGC 1 cut(s) 2943
AatII GACGTC 2 cut(s) 937, 3042
Acc36I ACCTGC 3 cut(s) 2542, 2764, 2943
AccB7I CCANNNNNTGG 2 cut(s) 2402, 4201
AccI GTMKAC 1 cut(s) 2682
AccII CGCG 1 cut(s) 4105
AclI AACGTT 2 cut(s) 186, 3992
AclWI GGATC 8 cut(s) 186, 246, 259, 1885, 2008, 2468, 2579, 3452
AcoI YGGCCR 4 cut(s) 785, 3024, 4106, 4192
AcsI RAATTY 9 cut(s) 217, 335, 900, 1412, 2058, 2183, 3029, 3087, 3902
AcuI CTGAAG 6 cut(s) 582, 1032, 2222, 2631, 3452, 3464
AcyI GRCGYC 3 cut(s) 17, 934, 3039
AfaI GTAC 8 cut(s) 245, 371, 648, 1917, 2812, 2928, 3186, 3564
AfiI CCNNNNNNNGG 8 cut(s) 733, 1900, 2378, 2402, 3298, 3781, 3856, 4201
AflII CTTAAG 1 cut(s) 4229
AflIII ACRYGT 1 cut(s) 3354
AjnI CCWGG 6 cut(s) 171, 1540, 1566, 1723, 2938, 3217
AjuI GAANNNNNNNTTGG 2 cut(s) 535, 567
Alw21I GWGCWC 5 cut(s) 623, 2620, 3538, 3939, 4098
Alw26I GTCTC 7 cut(s) 9, 1777, 1816, 2006, 2944, 3046, 3386
Alw44I GTGCAC 2 cut(s) 619, 3935
AlwI GGATC 8 cut(s) 186, 246, 259, 1885, 2008, 2468, 2579, 3452
AlwNI CAGNNNCTG 3 cut(s) 385, 2534, 3179
Ama87I CYCGRG 4 cut(s) 1926, 2052, 2727, 2918
AoxI GGCC 6 cut(s) 558, 785, 2966, 3024, 4106, 4192
ApaLI GTGCAC 2 cut(s) 619, 3935
ApoI RAATTY 9 cut(s) 217, 335, 900, 1412, 2058, 2183, 3029, 3087, 3902
AseI ATTAAT 2 cut(s) 1271, 3984
Asp700I GAANNNNTTC 2 cut(s) 2196, 3905
AspLEI GCGC 3 cut(s) 1851, 2108, 2394
AspS9I GGNCC 3 cut(s) 341, 559, 2967
AsuC2I CCSGG 4 cut(s) 1428, 2053, 2054, 2359
AsuHPI GGTGA 5 cut(s) 580, 803, 1255, 1262, 3910
AvaI CYCGRG 4 cut(s) 1926, 2052, 2727, 2918
AvaII GGWCC 1 cut(s) 341
AxyI CCTNAGG 2 cut(s) 2297, 2741
BaeGI GKGCMC 2 cut(s) 623, 3939
BaeI ACNNNNGTAYC 6 cut(s) 630, 663, 3327, 3360, 3419, 3452
BalI TGGCCA 1 cut(s) 4194
BamHI GGATCC 1 cut(s) 251
BanII GRGCYC 5 cut(s) 926, 2620, 3411, 3538, 3748
BauI CACGAG 1 cut(s) 2003
BbsI GAAGAC 3 cut(s) 1079, 3002, 3610
Bbv12I GWGCWC 5 cut(s) 623, 2620, 3538, 3939, 4098
BceAI ACGGC 2 cut(s) 2121, 3363
BcgI CGANNNNNNTGC 4 cut(s) 948, 982, 3795, 3829
BciT130I CCWGG 6 cut(s) 173, 1542, 1568, 1725, 2940, 3219
BciVI GTATCC 1 cut(s) 813
BclI TGATCA 1 cut(s) 2464
BcnI CCSGG 4 cut(s) 1428, 2053, 2054, 2359
BcoDI GTCTC 7 cut(s) 9, 1777, 1816, 2006, 2944, 3046, 3386
BfmI CTRYAG 4 cut(s) 665, 942, 1745, 4256
BfoI RGCGCY 3 cut(s) 1852, 2109, 2395
BfrI CTTAAG 1 cut(s) 4229
BfuAI ACCTGC 3 cut(s) 2542, 2764, 2943
BfuI GTATCC 1 cut(s) 813
BglII AGATCT 2 cut(s) 1259, 2731
BlpI GCTNAGC 2 cut(s) 2388, 3374
BmcAI AGTACT 1 cut(s) 3186
Bme18I GGWCC 1 cut(s) 341
BmeT110I CYCGRG 4 cut(s) 1926, 2052, 2727, 2918
BmgT120I GGNCC 3 cut(s) 341, 559, 2967
BmiI GGNNCC 7 cut(s) 253, 561, 2040, 2712, 2969, 3408, 3948
BmrI ACTGGG 1 cut(s) 2012
BmuI ACTGGG 1 cut(s) 2012
BpiI GAAGAC 3 cut(s) 1079, 3002, 3610
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 4 cut(s) 407, 1746, 3201, 3219
Bpu10I CCTNAGC 2 cut(s) 378, 3770
Bpu1102I GCTNAGC 2 cut(s) 2388, 3374
BpuEI CTTGAG 3 cut(s) 197, 3338, 3800
BpuMI CCSGG 4 cut(s) 1428, 2053, 2054, 2359
BsaAI YACGTR 1 cut(s) 3722
BsaBI GATNNNNATC 2 cut(s) 1470, 2574
BsaHI GRCGYC 3 cut(s) 17, 934, 3039
BsaI GGTCTC 1 cut(s) 1777
BsaWI WCCGGW 1 cut(s) 3849
BsaXI ACNNNNNCTCC 2 cut(s) 3525, 3555
Bsc4I CCNNNNNNNGG 8 cut(s) 733, 1900, 2378, 2402, 3298, 3781, 3856, 4201
Bse118I RCCGGY 2 cut(s) 831, 1764
Bse1I ACTGG 4 cut(s) 1382, 2018, 2524, 3910
Bse21I CCTNAGG 2 cut(s) 2297, 2741
Bse3DI GCAATG 6 cut(s) 372, 800, 839, 1830, 2114, 2635
Bse8I GATNNNNATC 2 cut(s) 1470, 2574
BseBI CCWGG 6 cut(s) 173, 1542, 1568, 1725, 2940, 3219
BseJI GATNNNNATC 2 cut(s) 1470, 2574
BseLI CCNNNNNNNGG 8 cut(s) 733, 1900, 2378, 2402, 3298, 3781, 3856, 4201
BseMI GCAATG 6 cut(s) 372, 800, 839, 1830, 2114, 2635
BseNI ACTGG 4 cut(s) 1382, 2018, 2524, 3910
BseRI GAGGAG 1 cut(s) 1082
BseSI GKGCMC 2 cut(s) 623, 3939
BseYI CCCAGC 2 cut(s) 1553, 1878
Bsh1236I CGCG 1 cut(s) 4105
Bsh1285I CGRYCG 1 cut(s) 97
BshFI GGCC 6 cut(s) 560, 787, 2968, 3026, 4108, 4194
BsiEI CGRYCG 1 cut(s) 97
BsiHKAI GWGCWC 5 cut(s) 623, 2620, 3538, 3939, 4098
BsiHKCI CYCGRG 4 cut(s) 1926, 2052, 2727, 2918
BsiSI CCGG 6 cut(s) 832, 1427, 1765, 2053, 2358, 3850
BslFI GGGAC 3 cut(s) 143, 480, 2847
BslI CCNNNNNNNGG 8 cut(s) 733, 1900, 2378, 2402, 3298, 3781, 3856, 4201
BsmAI GTCTC 7 cut(s) 9, 1777, 1816, 2006, 2944, 3046, 3386
BsmBI CGTCTC 2 cut(s) 3046, 3386
BsmFI GGGAC 3 cut(s) 143, 480, 2847
BsmI GAATGC 2 cut(s) 1325, 3274
BsnI GGCC 6 cut(s) 560, 787, 2968, 3026, 4108, 4194
Bso31I GGTCTC 1 cut(s) 1777
BsoBI CYCGRG 4 cut(s) 1926, 2052, 2727, 2918
Bsp1286I GDGCHC 8 cut(s) 623, 926, 2620, 3411, 3538, 3748, 3939, 4098
Bsp1407I TGTACA 1 cut(s) 369
Bsp1720I GCTNAGC 2 cut(s) 2388, 3374
BspANI GGCC 6 cut(s) 560, 787, 2968, 3026, 4108, 4194
BspFNI CGCG 1 cut(s) 4105
BspHI TCATGA 2 cut(s) 3556, 3835
BspLI GGNNCC 7 cut(s) 253, 561, 2040, 2712, 2969, 3408, 3948
BspMAI CTGCAG 1 cut(s) 946
BspMI ACCTGC 3 cut(s) 2542, 2764, 2943
BspPI GGATC 8 cut(s) 186, 246, 259, 1885, 2008, 2468, 2579, 3452
BspTI CTTAAG 1 cut(s) 4229
BspTNI GGTCTC 1 cut(s) 1777
BsrDI GCAATG 6 cut(s) 372, 800, 839, 1830, 2114, 2635
BsrFI RCCGGY 2 cut(s) 831, 1764
BsrGI TGTACA 1 cut(s) 369
BsrI ACTGG 4 cut(s) 1382, 2018, 2524, 3910
BssAI RCCGGY 2 cut(s) 831, 1764
BssNI GRCGYC 3 cut(s) 17, 934, 3039
BssSI CACGAG 1 cut(s) 2003
BssT1I CCWWGG 6 cut(s) 247, 815, 1440, 1528, 2481, 2706
Bst2BI CACGAG 1 cut(s) 2003
Bst2UI CCWGG 6 cut(s) 173, 1542, 1568, 1725, 2940, 3219
Bst4CI ACNGT 9 cut(s) 95, 625, 684, 2400, 2836, 3343, 3699, 3927, 4252
Bst6I CTCTTC 1 cut(s) 3389
BstACI GRCGYC 3 cut(s) 17, 934, 3039
BstAFI CTTAAG 1 cut(s) 4229
BstAPI GCANNNNNTGC 2 cut(s) 2534, 3278
BstAUI TGTACA 1 cut(s) 369
BstBAI YACGTR 1 cut(s) 3722
BstC8I GCNNGC 6 cut(s) 12, 678, 833, 837, 2134, 3486
BstDSI CCRYGG 3 cut(s) 2137, 2396, 4103
BstENI CCTNNNNNAGG 2 cut(s) 3296, 3779
BstFNI CGCG 1 cut(s) 4105
BstH2I RGCGCY 3 cut(s) 1852, 2109, 2395
BstHHI GCGC 3 cut(s) 1851, 2108, 2394
BstMAI GTCTC 7 cut(s) 9, 1777, 1816, 2006, 2944, 3046, 3386
BstMCI CGRYCG 1 cut(s) 97
BstNI CCWGG 6 cut(s) 173, 1542, 1568, 1725, 2940, 3219
BstNSI RCATGY 4 cut(s) 64, 645, 2981, 3358
BstSFI CTRYAG 4 cut(s) 665, 942, 1745, 4256
BstSLI GKGCMC 2 cut(s) 623, 3939
BstUI CGCG 1 cut(s) 4105
BstV2I GAAGAC 3 cut(s) 1079, 3002, 3610
BstX2I RGATCY 5 cut(s) 178, 251, 1259, 2584, 2731
BstYI RGATCY 5 cut(s) 178, 251, 1259, 2584, 2731
Bsu36I CCTNAGG 2 cut(s) 2297, 2741
BsuI GTATCC 1 cut(s) 813
BsuRI GGCC 6 cut(s) 560, 787, 2968, 3026, 4108, 4194
BtgI CCRYGG 3 cut(s) 2137, 2396, 4103
BtsI GCAGTG 5 cut(s) 1041, 1242, 1642, 3078, 3229
BveI ACCTGC 3 cut(s) 2542, 2764, 2943
Cac8I GCNNGC 6 cut(s) 12, 678, 833, 837, 2134, 3486
CaiI CAGNNNCTG 3 cut(s) 385, 2534, 3179
CciI TCATGA 2 cut(s) 3556, 3835
CfoI GCGC 3 cut(s) 1851, 2108, 2394
Cfr10I RCCGGY 2 cut(s) 831, 1764
Cfr13I GGNCC 3 cut(s) 341, 559, 2967
Cfr42I CCGCGG 1 cut(s) 4106
Cfr9I CCCGGG 1 cut(s) 2052
CseI GACGC 4 cut(s) 6, 52, 695, 2910
Csp6I GTAC 8 cut(s) 244, 370, 647, 1916, 2811, 2927, 3185, 3563
CviQI GTAC 8 cut(s) 244, 370, 647, 1916, 2811, 2927, 3185, 3563
DraI TTTAAA 1 cut(s) 222
EaeI YGGCCR 4 cut(s) 785, 3024, 4106, 4192
Eam1104I CTCTTC 1 cut(s) 3389
EarI CTCTTC 1 cut(s) 3389
EciI GGCGGA 1 cut(s) 530
Ecl136II GAGCTC 2 cut(s) 2618, 3536
Eco130I CCWWGG 6 cut(s) 247, 815, 1440, 1528, 2481, 2706
Eco24I GRGCYC 5 cut(s) 926, 2620, 3411, 3538, 3748
Eco31I GGTCTC 1 cut(s) 1777
Eco32I GATATC 2 cut(s) 433, 2594
Eco47I GGWCC 1 cut(s) 341
Eco53kI GAGCTC 2 cut(s) 2618, 3536
Eco57I CTGAAG 6 cut(s) 582, 1032, 2222, 2631, 3452, 3464
Eco81I CCTNAGG 2 cut(s) 2297, 2741
Eco88I CYCGRG 4 cut(s) 1926, 2052, 2727, 2918
EcoICRI GAGCTC 2 cut(s) 2618, 3536
EcoNI CCTNNNNNAGG 2 cut(s) 3296, 3779
EcoRII CCWGG 6 cut(s) 171, 1540, 1566, 1723, 2938, 3217
EcoRV GATATC 2 cut(s) 433, 2594
EcoT14I CCWWGG 6 cut(s) 247, 815, 1440, 1528, 2481, 2706
EcoT22I ATGCAT 1 cut(s) 3718
EcoT38I GRGCYC 5 cut(s) 926, 2620, 3411, 3538, 3748
ErhI CCWWGG 6 cut(s) 247, 815, 1440, 1528, 2481, 2706
Esp3I CGTCTC 2 cut(s) 3046, 3386
FalI AAGNNNNNCTT 4 cut(s) 453, 485, 3522, 3554
FaqI GGGAC 3 cut(s) 143, 480, 2847
FauI CCCGC 3 cut(s) 2345, 3477, 4110
FbaI TGATCA 1 cut(s) 2464
FblI GTMKAC 1 cut(s) 2682
FriOI GRGCYC 5 cut(s) 926, 2620, 3411, 3538, 3748
GlaI GCGC 3 cut(s) 1850, 2107, 2393
GsaI CCCAGC 2 cut(s) 1557, 1882
GsuI CTGGAG 4 cut(s) 407, 1746, 3201, 3219
HaeII RGCGCY 3 cut(s) 1852, 2109, 2395
HaeIII GGCC 6 cut(s) 560, 787, 2968, 3026, 4108, 4194
HapII CCGG 6 cut(s) 832, 1427, 1765, 2053, 2358, 3850
HgaI GACGC 4 cut(s) 6, 52, 695, 2910
HhaI GCGC 3 cut(s) 1851, 2108, 2394
Hin1I GRCGYC 3 cut(s) 17, 934, 3039
Hin6I GCGC 3 cut(s) 1849, 2106, 2392
HinP1I GCGC 3 cut(s) 1849, 2106, 2392
HincII GTYRAC 3 cut(s) 2247, 2517, 3453
HindII GTYRAC 3 cut(s) 2247, 2517, 3453
HindIII AAGCTT 3 cut(s) 2234, 2643, 4145
HpaII CCGG 6 cut(s) 832, 1427, 1765, 2053, 2358, 3850
HphI GGTGA 5 cut(s) 580, 803, 1255, 1262, 3910
Hpy99I CGWCG 2 cut(s) 22, 1715
HpyCH4III ACNGT 9 cut(s) 95, 625, 684, 2400, 2836, 3343, 3699, 3927, 4252
HpyCH4IV ACGT 6 cut(s) 186, 934, 3039, 3049, 3721, 3992
HpySE526I ACGT 6 cut(s) 186, 934, 3039, 3049, 3721, 3992
Hsp92I GRCGYC 3 cut(s) 17, 934, 3039
HspAI GCGC 3 cut(s) 1849, 2106, 2392
KroI GCCGGC 1 cut(s) 831
KroNI GCCGGC 1 cut(s) 833
Ksp22I TGATCA 1 cut(s) 2464
KspI CCGCGG 1 cut(s) 4106
LmnI GCTCC 7 cut(s) 86, 2851, 3140, 3406, 3533, 3733, 3946
MaeII ACGT 6 cut(s) 186, 934, 3039, 3049, 3721, 3992
MaeIII GTNAC 6 cut(s) 1515, 1650, 1873, 2441, 2836, 3799
MfeI CAATTG 3 cut(s) 1590, 2208, 4115
MflI RGATCY 5 cut(s) 178, 251, 1259, 2584, 2731
MhlI GDGCHC 8 cut(s) 623, 926, 2620, 3411, 3538, 3748, 3939, 4098
MlsI TGGCCA 1 cut(s) 4194
MluNI TGGCCA 1 cut(s) 4194
MlyI GAGTC 5 cut(s) 262, 977, 2663, 3668, 4026
MmeI TCCRAC 2 cut(s) 520, 3738
Mox20I TGGCCA 1 cut(s) 4194
Mph1103I ATGCAT 1 cut(s) 3718
MroNI GCCGGC 1 cut(s) 831
MroXI GAANNNNTTC 2 cut(s) 2196, 3905
MscI TGGCCA 1 cut(s) 4194
MslI CAYNNNNRTG 3 cut(s) 3355, 3840, 4044
Msp20I TGGCCA 1 cut(s) 4194
MspA1I CMGCKG 6 cut(s) 962, 2242, 3484, 3620, 4018, 4105
MspCI CTTAAG 1 cut(s) 4229
MspI CCGG 6 cut(s) 832, 1427, 1765, 2053, 2358, 3850
MunI CAATTG 3 cut(s) 1590, 2208, 4115
Mva1269I GAATGC 2 cut(s) 1325, 3274
MvaI CCWGG 6 cut(s) 173, 1542, 1568, 1725, 2940, 3219
MvnI CGCG 1 cut(s) 4105
NaeI GCCGGC 1 cut(s) 833
NciI CCSGG 4 cut(s) 1428, 2053, 2054, 2359
NgoMIV GCCGGC 1 cut(s) 831
NlaIV GGNNCC 7 cut(s) 253, 561, 2040, 2712, 2969, 3408, 3948
NmuCI GTSAC 3 cut(s) 1515, 2441, 3799
NsiI ATGCAT 1 cut(s) 3718
NspI RCATGY 4 cut(s) 64, 645, 2981, 3358
PaeR7I CTCGAG 3 cut(s) 1926, 2727, 2918
PagI TCATGA 2 cut(s) 3556, 3835
PaqCI CACCTGC 1 cut(s) 2943
PciI ACATGT 1 cut(s) 3354
PctI GAATGC 2 cut(s) 1325, 3274
PdiI GCCGGC 1 cut(s) 833
PdmI GAANNNNTTC 2 cut(s) 2196, 3905
PfeI GAWTC 6 cut(s) 353, 2414, 3074, 3475, 3508, 3812
PflFI GACNNNGTC 1 cut(s) 134
PflMI CCANNNNNTGG 2 cut(s) 2402, 4201
PfoI TCCNGGA 1 cut(s) 1426
PleI GAGTC 5 cut(s) 262, 977, 2663, 3667, 4026
PpsI GAGTC 5 cut(s) 262, 977, 2663, 3667, 4026
Ppu21I YACGTR 1 cut(s) 3722
PscI ACATGT 1 cut(s) 3354
PshBI ATTAAT 2 cut(s) 1271, 3984
PsiI TTATAA 2 cut(s) 191, 1955
Psp124BI GAGCTC 2 cut(s) 2620, 3538
Psp1406I AACGTT 2 cut(s) 186, 3992
Psp6I CCWGG 6 cut(s) 171, 1540, 1566, 1723, 2938, 3217
PspFI CCCAGC 2 cut(s) 1553, 1878
PspGI CCWGG 6 cut(s) 171, 1540, 1566, 1723, 2938, 3217
PspN4I GGNNCC 7 cut(s) 253, 561, 2040, 2712, 2969, 3408, 3948
PspPI GGNCC 3 cut(s) 341, 559, 2967
PspXI VCTCGAGB 2 cut(s) 1926, 2918
PstI CTGCAG 1 cut(s) 946
PstNI CAGNNNCTG 3 cut(s) 385, 2534, 3179
PsuI RGATCY 5 cut(s) 178, 251, 1259, 2584, 2731
PsyI GACNNNGTC 1 cut(s) 134
PvuII CAGCTG 2 cut(s) 962, 3620
RsaI GTAC 8 cut(s) 245, 371, 648, 1917, 2812, 2928, 3186, 3564
RsaNI GTAC 8 cut(s) 244, 370, 647, 1916, 2811, 2927, 3185, 3563
RseI CAYNNNNRTG 3 cut(s) 3355, 3840, 4044
SacI GAGCTC 2 cut(s) 2620, 3538
SacII CCGCGG 1 cut(s) 4106
Sau96I GGNCC 3 cut(s) 341, 559, 2967
ScaI AGTACT 1 cut(s) 3186
SchI GAGTC 5 cut(s) 262, 977, 2663, 3668, 4026
SduI GDGCHC 8 cut(s) 623, 926, 2620, 3411, 3538, 3748, 3939, 4098
SfcI CTRYAG 4 cut(s) 665, 942, 1745, 4256
Sfr274I CTCGAG 3 cut(s) 1926, 2727, 2918
Sfr303I CCGCGG 1 cut(s) 4106
SgrBI CCGCGG 1 cut(s) 4106
SinI GGWCC 1 cut(s) 341
SlaI CTCGAG 3 cut(s) 1926, 2727, 2918
SmaI CCCGGG 1 cut(s) 2054
SmiI ATTTAAAT 1 cut(s) 222
SmiMI CAYNNNNRTG 3 cut(s) 3355, 3840, 4044
SmlI CTYRAG 7 cut(s) 212, 1926, 2727, 2918, 3317, 3779, 4229
SmoI CTYRAG 7 cut(s) 212, 1926, 2727, 2918, 3317, 3779, 4229
SspI AATATT 3 cut(s) 166, 2698, 3503
SstI GAGCTC 2 cut(s) 2620, 3538
StyI CCWWGG 6 cut(s) 247, 815, 1440, 1528, 2481, 2706
SwaI ATTTAAAT 1 cut(s) 222
TaaI ACNGT 9 cut(s) 95, 625, 684, 2400, 2836, 3343, 3699, 3927, 4252
TaiI ACGT 6 cut(s) 189, 937, 3042, 3052, 3724, 3995
TatI WGTACW 2 cut(s) 369, 3184
TauI GCSGC 3 cut(s) 17, 2279, 4108
TfiI GAWTC 6 cut(s) 353, 2414, 3074, 3475, 3508, 3812
TseFI GTSAC 3 cut(s) 1515, 2441, 3799
Tsp45I GTSAC 3 cut(s) 1515, 2441, 3799
TspGWI ACGGA 1 cut(s) 1091
TspMI CCCGGG 1 cut(s) 2052
Tth111I GACNNNGTC 1 cut(s) 134
Van91I CCANNNNNTGG 2 cut(s) 2402, 4201
Vha464I CTTAAG 1 cut(s) 4229
VneI GTGCAC 2 cut(s) 619, 3935
VpaK11BI GGWCC 1 cut(s) 341
VspI ATTAAT 2 cut(s) 1271, 3984
XagI CCTNNNNNAGG 2 cut(s) 3296, 3779
XapI RAATTY 9 cut(s) 217, 335, 900, 1412, 2058, 2183, 3029, 3087, 3902
XbaI TCTAGA 2 cut(s) 2602, 2734
XceI RCATGY 4 cut(s) 64, 645, 2981, 3358
XhoI CTCGAG 3 cut(s) 1926, 2727, 2918
XmaI CCCGGG 1 cut(s) 2052
XmiI GTMKAC 1 cut(s) 2682
XmnI GAANNNNTTC 2 cut(s) 2196, 3905
ZraI GACGTC 2 cut(s) 935, 3040
ZrmI AGTACT 1 cut(s) 3186
Zsp2I ATGCAT 1 cut(s) 3718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.