RchiOBHm_Chr4g0446521

Serine threonine-protein kinase sepA-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0006663
Physical Location & Seq
Forward (+)
66659215 .. 66671283
12069 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41404

Sequence Viewer

Length: 4266 bp
ATGTCTCGGCAGGCGGCGTCGGCCCACTTCCACAAATCCAAGACTCTCGATAACAAATACATGCTTGGAGATGAGATTGGAAAGGGAGCATATGGTCGAGTTTACAAGGGTTTGGATTTGGAGAATGGAGACTTTGTTGCAATTAAACAGGTTTCGTTGGAGAATATTGCGCAGGAGGATCTCAACGTTATAATGCAAGAGATTGATTTACTCAAGAATTTAAATCACAAAAACATTGTGAAATATCTTGGATCCTTAAAGACAAGGACACATCTTCACATAGTCCTAGAATATGTGGAGAATGGCTCACTTGCAAACATTATTAAGCCAAATAAGTTTGGACCTTTTCCAGAATCACTGGTAGCTGTGTACATTGCTCAGGTTTTGGAAGGCTTGGTGTATTTACATGAACAGGGTGTAATCCATCGGGATATCAAGGGTGCAAATATATTGACAACTAAAGAGGGCCTTGTGAAACTTGCTGATTTTGGTGTTGCTACAAAATTGACCGAGGCTGATGTGAATACACATTCAGTTGTTGGAACACCATATTGGATGGCCCCAGAGGTGATTGAAATGTCGGGGGTTTGTGCTGCTTCAGATATTTGGAGTGTTGGCTGCACTGTTATTGAACTTCTTACATGTGTACCTCCTTATTATGATTTACAGCCAATGCCAGCACTATTTCGCATTGTTCAGGATGAACATCCTCCAATTCCTGACAGTCTATCTCCTGACATTACTGATTTTCTGTGTCAATGCTTTAAAAAGGATGCTAGGCACCGGCCTGATGCAAAAACACTGTTATCTCATCCTTGGATACAAAACTGCAGACGAGTGTTGCAATCATCTATTCGTCACAGTGGAACATTAAGAGATATACAGGAAGATGTTTCTAAGGGTGCAGAACTCTCCAATGGAGATATTCGGAGCTCTGGCGAAAGTCCGGCAGAGAAAACAGAAGAAGCAGCTTCTGCTATTAAAGCAGACTCTAGGAAAGAGTTATTATCAACCGGGGTTTCTGATATGAGAAAATCTGGCGAGGATCCTGCTTCAGATGTGAAATTTGTTGAGGAAAAAGAAGATGCTTTAGAAAATGATCTGACGGATCAGGCTCCCACCTTAGCCATACATGAAAAATCATCTTTGCCAAATGGTTCTGGCCGAATATCTTCTAATAAAGAATTGTCTGCCTCCGAGCCAGCTGAGCTTGATGGTCGAGCACATACAGCCAACGATGATGAACCACCAATGAATGGGGAGGTTAAATCTCCTGAATTAACGACAAATAGTATAACTGGGAAACATGGAAAAGGGAATTCTATTGGGTTTAGACCATTTGGTTTTGGAGCAAGAAATCAGGATGGTGCTTTTCAAAAGGCTGCAAAGATGCCAGTCGGTTTGGAAGGGAATGAACTCAGTAAATTTAGTGACACTCCAGGAGATGCTTCCTTGGATGATCTATTTCATCCATTGGATAAGCATCCCGATGGTAGGGCAACTGAAGCTTCAACATCTTCTTCAATTTCACATTTGAACCAAGGAAATACATCTGTAAACGATGCTGGGAAAAGTGACCTGGCTATGAAATTAAGGGCTACAATTGCTCAGAAGCAAATGGAGAGTGAAATGGGTCAGGCTAATGGTAGTGGTGGTAACCTGTTACAGCTGATGATGGGTGTTCTCCAAGATGATGTGATTGATATTGGTGGTTTGGTATTTGATGAAAAGTTGCCCGGAGAAAACCTTTTTCCCCTACAGGCTGTTGAGTTTAGCCGGTTGGTTGGATCATTGAAACTAGATGAATCAGAAGATGTTGTTGTCTCTGCCTGCCAGAAACTTATTGCTATATTCCATCAGCGTCCAGAGCAGAAAATTGTTTTTGTCACACAGCATGGTTTGCTCCCTTTAATGGAATTGCTTGAAGTTCCTAAAACTCGAGTTATATGTTCTGTGCTTCAAATCATAAACCAGATAATCAAAGACAACACCGATTTCCAGGAAAATGCTTGTCATGTTGGTCTGATCCCAGTGGTAATGAGCTTTGCGGTACCTAATTATTCTCGAGAAATTCGTATGGAAGCAGCTTATTTCTTGCAGCAGCTTTGTCAGTCAAGCCCATTGACTTTGCAAATGTTCATAGCTTGCCGTGGAATACCTGTTCTGGTGGGCTTTTTAGAGGCTGATTATGCAAAATTCAGGGAAATGGTTCATCTAGCTATTGACGGCATGTGGCAAGTCTTTAAGCTTCAGCGATCAACCCCTAGGAATGATTTCTGTCGCATAGCTGCAAAGAATGGAATACTCCTGAGGCTTATTAACACCCTTTATAGCTTGAATGAGGCAACACGTTTAGCTTCCATTTCTGGTGGGGGTGGATTTCCTCTAGACGGTTCTGCTCAGCGCCCACGGTCTGGTTCATTGGATTCTGGTCATCCCATTTTTGCTCAGAGTGATGGACCACTTCCTGATCATAATGATCACTCTAAGGTTAGGCATGTTGTAAATGATTTGCATTTGTCTAGTGGAACAGAACCTGCACGTGCTTCAACTTCTAATTCTCAAAGGTCAGATGCCAATCAATCAGATCTGCGGTATCATCATCTAGATACTGATAGACCTCAATCTAGCAATGCAGTAGTAGATGCTTCAGTTTCTTCTAAATTGCAAGACCCAACATCCGCAGATAAAGTAGTCAATATTTCAGCGAAGGAACCTTCAACTACTTCTCGAGGAGATCTTGACCTTAGGCAGCAGCGAGCCCCTATATCTCTTCATAGATCATCCACTGATAGATCTCCTAAAATGATGGAAGCTACATCAAATGGCTTATCAACAACAGCACCTACTCAACAGGAGCAAGTTCGACCTCTTCTTAGCTTGTTGGAGAAAGAGCCCCCTTCAAGACACTTTTCTGGTCAGCTCGATTATGTACGCCACCTTCCTGGGTTGGAGAGACATGAAAGTATACTGCCCCTTTTACATGCATCTAATGAAAAGAAAACAAATGGTGAATTAGATTTCCTTATGGCAGAATTTGCAGATGTCTCTCAACGTGGTAAAGAGAAAGGGAATCTTGATTCTACTACTAGAATTCCCCCCAAAACAGTAAATAAGGAGGTGGGAACACTAGCATCTAACAAAGGAGCTGCTTCCACCTCTGGGATAGCATCTCAGACAGCATCAGGGGTGTTGTCTGGTTCGGGTGTTCTAAATGCTAGGCCAGGCAGTGCAACATCATCAGGTTTACTCTCGCACATGGTTTCGACATTGAATGCAGACGTTGCAAGGGAATACCTAGAAAAGGTGGCAGATCTTCTGCTTGAGTTTGCACGAGCAGATACTACTGTAAAATCATACATGTGTAGCCAAAGTTTGCTCAGCCGTCTTTTTCAGATGTTTAATAGGGTGGAGCCCCCAATTCTTTTAAAGATACTAAAGTGTGTCAACCATTTATCAACCGATCCAAACTGCTTAGAGAATCTTCAGCGGGCAGATGCAATCAAATATCTGATTCCAAATCTTGAACTCAAAGAAGGATCTCTTGTATCTCAAATACATCATGAGGTCCTAAATGCCTTGTTCAATCTGTGTAAGATTAACAAGAGGAGACAGGAACAAGCAGCTGAGAATGGAATAATTCCACACTTAATGCACTTTATTGAGTCTAACTCTCCTCTGAAACAATATGCGTTGCCTTTGCTCTGTGATATGGCTCATGCTTCACGTAATTCAAGAGAGCAATTAAGGGCTCATGGTGGGTTGGATGTGTACTTGAGCCTTCTAGAGGATGAGCTTTGGTCTGTGACTGCATTGGATTCAATTGCTGTTTGCTTAGCTCATGACAATGACAACCGGAAGGTAGAACAAGCACTGTTGAGAAAGGATGCAGTTCAGAAATTGGTGAAATTCTTCCAGTGCTGTCCAGAACAGTATTTCGTGCACATATTGGAGCCATTTTTGAAAATTATCACGAAATCGTCTCGAATTAATACGACATTAGCTGTTAATGGTTTGACACCACTGCTTATCACAAGACTTGACCATCAGGATGCTATTGCCCGTCTAAATCTTCTTAAACTGATAAAGGCTGTTTATGAGCACCACCCACGGCCTAAGCAATTGATTGTGGAGAATGATCTGCCTCAGAAGCTTCGGAATCTAATAGAGGAACGCAGAGATGGGCAACGTTCTGGGGGCCAAGTATTGGTGAAACAAATGGCTACCTCCTTGCTGAAAGCACTCCATATTAACACCGTATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000278 GO:0000281 GO:0000910 GO:0001558 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005813 GO:0005815 GO:0005829 GO:0005856 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007049 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0008361 GO:0009893 GO:0009987 GO:0010324 GO:0010562 GO:0010604 GO:0015630 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022402 GO:0022603 GO:0022604 GO:0023014 GO:0023052 GO:0030587 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032147 GO:0032268 GO:0032270 GO:0032502 GO:0032506 GO:0032535 GO:0033554 GO:0033674 GO:0035556 GO:0036089 GO:0036211 GO:0040008 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0045995 GO:0046777 GO:0048518 GO:0048522 GO:0048638 GO:0048856 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051246 GO:0051247 GO:0051301 GO:0051302 GO:0051338 GO:0051347 GO:0051510 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0061024 GO:0061387 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090066 GO:0090702 GO:0099024 GO:0099120 GO:0140096 GO:1901564 GO:1903047 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1421

Amino Acids

156.26

Weight (kDa)

6.14

Isoelectric Point (pI)

43.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 21 - 274 5.3e-73 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 22 - 270 7e-51 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 191
Acc16I TGCGCA 1 cut(s) 171
Acc36I ACCTGC 1 cut(s) 2545
Acc65I GGTACC 1 cut(s) 2050
AccB1I GGYRCC 2 cut(s) 780, 2050
AccB7I CCANNNNNTGG 3 cut(s) 1256, 2414, 4207
AccI GTMKAC 1 cut(s) 2968
AciI CCGC 5 cut(s) 14, 2048, 2593, 2682, 3490
AclI AACGTT 2 cut(s) 186, 4189
AcoI YGGCCR 1 cut(s) 1162
AcsI RAATTY 9 cut(s) 217, 1064, 1318, 1424, 2070, 2195, 3035, 3093, 3908
AcuI CTGAAG 6 cut(s) 582, 1038, 1524, 2234, 2634, 3470
AcvI CACGTG 1 cut(s) 2543
AcyI GRCGYC 1 cut(s) 17
AfaI GTAC 5 cut(s) 371, 648, 2052, 2934, 3773
AfiI CCNNNNNNNGG 9 cut(s) 1256, 1494, 1912, 2390, 2414, 3162, 3304, 3787, 4207
AflIII ACRYGT 3 cut(s) 641, 2348, 3360
AjnI CCWGG 5 cut(s) 1438, 1578, 1998, 2944, 3223
AjuI GAANNNNNNNTTGG 2 cut(s) 535, 567
AloI GAACNNNNNNTCC 2 cut(s) 2145, 2177
Alw21I GWGCWC 4 cut(s) 935, 1225, 3945, 4104
Alw26I GTCTC 7 cut(s) 9, 123, 1828, 2950, 3052, 3604, 3987
Alw44I GTGCAC 1 cut(s) 3941
AlwNI CAGNNNCTG 2 cut(s) 974, 2537
Ama87I CYCGRG 3 cut(s) 1938, 2064, 2730
AoxI GGCC 8 cut(s) 21, 466, 558, 785, 1162, 3221, 4112, 4198
ApaLI GTGCAC 1 cut(s) 3941
ApoI RAATTY 9 cut(s) 217, 1064, 1318, 1424, 2070, 2195, 3035, 3093, 3908
AseI ATTAAT 1 cut(s) 3990
Asp700I GAANNNNTTC 4 cut(s) 1171, 2208, 2273, 3911
Asp718I GGTACC 1 cut(s) 2050
AspA2I CCTAGG 1 cut(s) 2264
AspLEI GCGC 2 cut(s) 172, 2406
AspS9I GGNCC 7 cut(s) 22, 341, 466, 559, 2459, 3568, 4198
AsuC2I CCSGG 2 cut(s) 1015, 1737
AsuHPI GGTGA 4 cut(s) 580, 3023, 3916, 4222
AvaI CYCGRG 3 cut(s) 1938, 2064, 2730
AvaII GGWCC 3 cut(s) 341, 2459, 3568
AvrII CCTAGG 1 cut(s) 2264
AxyI CCTNAGG 2 cut(s) 2309, 2747
BaeGI GKGCMC 1 cut(s) 3945
BaeI ACNNNNGTAYC 6 cut(s) 630, 663, 3333, 3366, 3425, 3458
BamHI GGATCC 2 cut(s) 251, 1045
BanI GGYRCC 2 cut(s) 780, 2050
BanII GRGCYC 5 cut(s) 935, 2764, 2898, 3417, 3754
BarI GAAGNNNNNNTAC 2 cut(s) 2925, 2957
BauI CACGAG 1 cut(s) 3333
BbrPI CACGTG 1 cut(s) 2543
Bbv12I GWGCWC 4 cut(s) 935, 1225, 3945, 4104
BceAI ACGGC 4 cut(s) 2133, 2242, 3369, 4127
BcgI CGANNNNNNTGC 2 cut(s) 1031, 1065
BciT130I CCWGG 5 cut(s) 1440, 1580, 2000, 2946, 3225
BciVI GTATCC 1 cut(s) 813
BclI TGATCA 2 cut(s) 2470, 2479
BcnI CCSGG 2 cut(s) 1015, 1737
BcoDI GTCTC 7 cut(s) 9, 123, 1828, 2950, 3052, 3604, 3987
BfmI CTRYAG 2 cut(s) 829, 1757
BfoI RGCGCY 1 cut(s) 2407
BfuAI ACCTGC 1 cut(s) 2545
BfuI GTATCC 1 cut(s) 813
BglII AGATCT 4 cut(s) 2587, 2737, 2795, 3313
BlnI CCTAGG 1 cut(s) 2264
BlpI GCTNAGC 4 cut(s) 1206, 2400, 3380, 3835
Bme1390I CCNGG 7 cut(s) 1015, 1440, 1580, 1737, 2000, 2946, 3225
Bme18I GGWCC 3 cut(s) 341, 2459, 3568
BmeT110I CYCGRG 3 cut(s) 1938, 2064, 2730
BmgT120I GGNCC 7 cut(s) 22, 341, 466, 559, 2459, 3568, 4198
BmrFI CCNGG 7 cut(s) 1015, 1440, 1580, 1737, 2000, 2946, 3225
BmrI ACTGGG 2 cut(s) 1308, 2024
BmuI ACTGGG 2 cut(s) 1308, 2024
BplI GAGNNNNNCTC 4 cut(s) 290, 322, 3656, 3688
BpmI CTGGAG 1 cut(s) 1422
Bpu10I CCTNAGC 3 cut(s) 378, 1123, 4116
Bpu1102I GCTNAGC 4 cut(s) 1206, 2400, 3380, 3835
BpuEI CTTGAG 3 cut(s) 197, 3344, 3796
BpuMI CCSGG 2 cut(s) 1015, 1737
BsaAI YACGTR 2 cut(s) 2543, 3728
BsaBI GATNNNNATC 3 cut(s) 705, 1482, 2577
BsaHI GRCGYC 1 cut(s) 17
BsaWI WCCGGW 1 cut(s) 3855
BsaXI ACNNNNNCTCC 2 cut(s) 120, 150
Bsc4I CCNNNNNNNGG 9 cut(s) 1256, 1494, 1912, 2390, 2414, 3162, 3304, 3787, 4207
Bse118I RCCGGY 2 cut(s) 783, 1776
Bse1I ACTGG 5 cut(s) 363, 1303, 1394, 2030, 3916
Bse21I CCTNAGG 2 cut(s) 2309, 2747
Bse3DI GCAATG 2 cut(s) 372, 2638
Bse8I GATNNNNATC 3 cut(s) 705, 1482, 2577
BseBI CCWGG 5 cut(s) 1440, 1580, 2000, 2946, 3225
BseJI GATNNNNATC 3 cut(s) 705, 1482, 2577
BseLI CCNNNNNNNGG 9 cut(s) 1256, 1494, 1912, 2390, 2414, 3162, 3304, 3787, 4207
BseMI GCAATG 2 cut(s) 372, 2638
BseNI ACTGG 5 cut(s) 363, 1303, 1394, 2030, 3916
BseRI GAGGAG 3 cut(s) 2748, 3622, 3666
BseSI GKGCMC 1 cut(s) 3945
BseYI CCCAGC 1 cut(s) 1565
BsgI GTGCAG 3 cut(s) 604, 924, 2523
BshFI GGCC 8 cut(s) 23, 468, 560, 787, 1164, 3223, 4114, 4200
BshNI GGYRCC 2 cut(s) 780, 2050
BsiHKAI GWGCWC 4 cut(s) 935, 1225, 3945, 4104
BsiHKCI CYCGRG 3 cut(s) 1938, 2064, 2730
BsiSI CCGG 6 cut(s) 784, 947, 1014, 1737, 1777, 3856
BslI CCNNNNNNNGG 9 cut(s) 1256, 1494, 1912, 2390, 2414, 3162, 3304, 3787, 4207
BsmAI GTCTC 7 cut(s) 9, 123, 1828, 2950, 3052, 3604, 3987
BsmBI CGTCTC 1 cut(s) 3987
BsmI GAATGC 1 cut(s) 3280
BsnI GGCC 8 cut(s) 23, 468, 560, 787, 1164, 3223, 4114, 4200
BsoBI CYCGRG 3 cut(s) 1938, 2064, 2730
Bsp1286I GDGCHC 8 cut(s) 935, 1225, 2764, 2898, 3417, 3754, 3945, 4104
Bsp1407I TGTACA 1 cut(s) 369
Bsp1720I GCTNAGC 4 cut(s) 1206, 2400, 3380, 3835
BspACI CCGC 5 cut(s) 14, 2048, 2593, 2682, 3490
BspANI GGCC 8 cut(s) 23, 468, 560, 787, 1164, 3223, 4114, 4200
BspHI TCATGA 2 cut(s) 3562, 3841
BspMAI CTGCAG 1 cut(s) 833
BspMI ACCTGC 1 cut(s) 2545
BspT107I GGYRCC 2 cut(s) 780, 2050
BsrDI GCAATG 2 cut(s) 372, 2638
BsrFI RCCGGY 2 cut(s) 783, 1776
BsrGI TGTACA 1 cut(s) 369
BsrI ACTGG 5 cut(s) 363, 1303, 1394, 2030, 3916
BssAI RCCGGY 2 cut(s) 783, 1776
BssNAI GTATAC 1 cut(s) 2969
BssNI GRCGYC 1 cut(s) 17
BssSI CACGAG 1 cut(s) 3333
BssT1I CCWWGG 4 cut(s) 815, 1452, 1540, 2264
Bst1107I GTATAC 1 cut(s) 2969
Bst2BI CACGAG 1 cut(s) 3333
Bst2UI CCWGG 5 cut(s) 1440, 1580, 2000, 2946, 3225
Bst6I CTCTTC 2 cut(s) 2778, 2877
BstACI GRCGYC 1 cut(s) 17
BstAPI GCANNNNNTGC 4 cut(s) 974, 1900, 3038, 3284
BstAUI TGTACA 1 cut(s) 369
BstBAI YACGTR 2 cut(s) 2543, 3728
BstC8I GCNNGC 7 cut(s) 12, 678, 1203, 1831, 2146, 2760, 3492
BstDSI CCRYGG 3 cut(s) 2149, 2408, 4109
BstEII GGTNACC 1 cut(s) 1655
BstENI CCTNNNNNAGG 2 cut(s) 3302, 3785
BstH2I RGCGCY 1 cut(s) 2407
BstHHI GCGC 2 cut(s) 172, 2406
BstMAI GTCTC 7 cut(s) 9, 123, 1828, 2950, 3052, 3604, 3987
BstNI CCWGG 5 cut(s) 1440, 1580, 2000, 2946, 3225
BstNSI RCATGY 6 cut(s) 64, 645, 2233, 2501, 2987, 3364
BstPI GGTNACC 1 cut(s) 1655
BstSCI CCNGG 7 cut(s) 1013, 1438, 1578, 1735, 1998, 2944, 3223
BstSFI CTRYAG 2 cut(s) 829, 1757
BstSLI GKGCMC 1 cut(s) 3945
BstX2I RGATCY 8 cut(s) 178, 251, 1045, 2587, 2737, 2795, 3313, 3539
BstXI CCANNNNNNTGG 1 cut(s) 2945
BstYI RGATCY 8 cut(s) 178, 251, 1045, 2587, 2737, 2795, 3313, 3539
BstZ17I GTATAC 1 cut(s) 2969
Bsu36I CCTNAGG 2 cut(s) 2309, 2747
BsuI GTATCC 1 cut(s) 813
BsuRI GGCC 8 cut(s) 23, 468, 560, 787, 1164, 3223, 4114, 4200
BtgI CCRYGG 3 cut(s) 2149, 2408, 4109
BtsI GCAGTG 2 cut(s) 3235, 4022
BveI ACCTGC 1 cut(s) 2545
Cac8I GCNNGC 7 cut(s) 12, 678, 1203, 1831, 2146, 2760, 3492
CaiI CAGNNNCTG 2 cut(s) 974, 2537
CciI TCATGA 2 cut(s) 3562, 3841
CfoI GCGC 2 cut(s) 172, 2406
Cfr10I RCCGGY 2 cut(s) 783, 1776
Cfr13I GGNCC 7 cut(s) 22, 341, 466, 559, 2459, 3568, 4198
CseI GACGC 2 cut(s) 6, 1850
Csp6I GTAC 5 cut(s) 370, 647, 2051, 2933, 3772
CviQI GTAC 5 cut(s) 370, 647, 2051, 2933, 3772
DraI TTTAAA 3 cut(s) 222, 766, 3429
EaeI YGGCCR 1 cut(s) 1162
Eam1104I CTCTTC 2 cut(s) 2778, 2877
EarI CTCTTC 2 cut(s) 2778, 2877
Ecl136II GAGCTC 1 cut(s) 933
Eco130I CCWWGG 4 cut(s) 815, 1452, 1540, 2264
Eco24I GRGCYC 5 cut(s) 935, 2764, 2898, 3417, 3754
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 3 cut(s) 341, 2459, 3568
Eco53kI GAGCTC 1 cut(s) 933
Eco57I CTGAAG 6 cut(s) 582, 1038, 1524, 2234, 2634, 3470
Eco72I CACGTG 1 cut(s) 2543
Eco81I CCTNAGG 2 cut(s) 2309, 2747
Eco88I CYCGRG 3 cut(s) 1938, 2064, 2730
Eco91I GGTNACC 1 cut(s) 1655
EcoICRI GAGCTC 1 cut(s) 933
EcoNI CCTNNNNNAGG 2 cut(s) 3302, 3785
EcoO109I RGGNCCY 2 cut(s) 466, 3568
EcoO65I GGTNACC 1 cut(s) 1655
EcoRI GAATTC 2 cut(s) 1318, 3093
EcoRII CCWGG 5 cut(s) 1438, 1578, 1998, 2944, 3223
EcoRV GATATC 1 cut(s) 433
EcoT14I CCWWGG 4 cut(s) 815, 1452, 1540, 2264
EcoT22I ATGCAT 1 cut(s) 2989
EcoT38I GRGCYC 5 cut(s) 935, 2764, 2898, 3417, 3754
ErhI CCWWGG 4 cut(s) 815, 1452, 1540, 2264
Esp3I CGTCTC 1 cut(s) 3987
FalI AAGNNNNNCTT 6 cut(s) 453, 485, 3060, 3092, 3528, 3560
FauI CCCGC 1 cut(s) 3483
FauNDI CATATG 1 cut(s) 91
FbaI TGATCA 2 cut(s) 2470, 2479
FblI GTMKAC 1 cut(s) 2968
FriOI GRGCYC 5 cut(s) 935, 2764, 2898, 3417, 3754
FspI TGCGCA 1 cut(s) 171
GlaI GCGC 2 cut(s) 171, 2405
GsaI CCCAGC 1 cut(s) 1569
GsuI CTGGAG 1 cut(s) 1422
HaeII RGCGCY 1 cut(s) 2407
HaeIII GGCC 8 cut(s) 23, 468, 560, 787, 1164, 3223, 4114, 4200
HapII CCGG 6 cut(s) 784, 947, 1014, 1737, 1777, 3856
HgaI GACGC 2 cut(s) 6, 1850
HhaI GCGC 2 cut(s) 172, 2406
Hin1I GRCGYC 1 cut(s) 17
Hin6I GCGC 2 cut(s) 170, 2404
HinP1I GCGC 2 cut(s) 170, 2404
HincII GTYRAC 1 cut(s) 3448
HindII GTYRAC 1 cut(s) 3448
HindIII AAGCTT 3 cut(s) 1506, 2246, 4151
HpaII CCGG 6 cut(s) 784, 947, 1014, 1737, 1777, 3856
HphI GGTGA 4 cut(s) 580, 3023, 3916, 4222
Hpy166II GTNNAC 9 cut(s) 103, 370, 647, 1558, 2969, 3248, 3448, 3772, 3943
Hpy8I GTNNAC 9 cut(s) 103, 370, 647, 1558, 2969, 3248, 3448, 3772, 3943
Hpy99I CGWCG 1 cut(s) 22
HpyAV CCTTC 9 cut(s) 383, 1400, 2704, 2727, 2910, 2951, 3530, 3791, 3853
HpyCH4IV ACGT 7 cut(s) 186, 2350, 2542, 3055, 3282, 3727, 4189
HpySE526I ACGT 7 cut(s) 186, 2350, 2542, 3055, 3282, 3727, 4189
Hsp92I GRCGYC 1 cut(s) 17
HspAI GCGC 2 cut(s) 170, 2404
KpnI GGTACC 1 cut(s) 2054
Ksp22I TGATCA 2 cut(s) 2470, 2479
LmnI GCTCC 9 cut(s) 86, 930, 1120, 1349, 1908, 2857, 3146, 3412, 3952
MaeII ACGT 7 cut(s) 186, 2350, 2542, 3055, 3282, 3727, 4189
MaeIII GTNAC 7 cut(s) 857, 1430, 1574, 1655, 1662, 1885, 3805
MfeI CAATTG 3 cut(s) 1602, 3822, 4121
MflI RGATCY 8 cut(s) 178, 251, 1045, 2587, 2737, 2795, 3313, 3539
MhlI GDGCHC 8 cut(s) 935, 1225, 2764, 2898, 3417, 3754, 3945, 4104
MlyI GAGTC 3 cut(s) 37, 983, 3674
MmeI TCCRAC 6 cut(s) 138, 520, 1765, 2865, 2931, 3744
Mph1103I ATGCAT 1 cut(s) 2989
MroXI GAANNNNTTC 4 cut(s) 1171, 2208, 2273, 3911
MslI CAYNNNNRTG 4 cut(s) 1488, 3361, 3846, 4050
MspA1I CMGCKG 4 cut(s) 1205, 1669, 3490, 3626
MspI CCGG 6 cut(s) 784, 947, 1014, 1737, 1777, 3856
MspR9I CCNGG 7 cut(s) 1015, 1440, 1580, 1737, 2000, 2946, 3225
MunI CAATTG 3 cut(s) 1602, 3822, 4121
Mva1269I GAATGC 1 cut(s) 3280
MvaI CCWGG 5 cut(s) 1440, 1580, 2000, 2946, 3225
NciI CCSGG 2 cut(s) 1015, 1737
NdeI CATATG 1 cut(s) 91
NmuCI GTSAC 5 cut(s) 857, 1430, 1574, 1885, 3805
NsbI TGCGCA 1 cut(s) 171
NsiI ATGCAT 1 cut(s) 2989
NspI RCATGY 6 cut(s) 64, 645, 2233, 2501, 2987, 3364
PaeR7I CTCGAG 3 cut(s) 1938, 2064, 2730
PagI TCATGA 2 cut(s) 3562, 3841
PciI ACATGT 2 cut(s) 641, 3360
PcsI WCGNNNNNNNCGW 1 cut(s) 2071
PctI GAATGC 1 cut(s) 3280
PdmI GAANNNNTTC 4 cut(s) 1171, 2208, 2273, 3911
PfeI GAWTC 9 cut(s) 353, 1805, 2426, 3073, 3080, 3481, 3514, 3818, 4159
PflMI CCANNNNNTGG 3 cut(s) 1256, 2414, 4207
PfoI TCCNGGA 2 cut(s) 1438, 1998
PleI GAGTC 3 cut(s) 37, 983, 3673
PmaCI CACGTG 1 cut(s) 2543
PmlI CACGTG 1 cut(s) 2543
PpsI GAGTC 3 cut(s) 37, 983, 3673
Ppu21I YACGTR 2 cut(s) 2543, 3728
PpuMI RGGWCCY 1 cut(s) 3568
PscI ACATGT 2 cut(s) 641, 3360
PshBI ATTAAT 1 cut(s) 3990
PsiI TTATAA 1 cut(s) 191
Psp124BI GAGCTC 1 cut(s) 935
Psp1406I AACGTT 2 cut(s) 186, 4189
Psp5II RGGWCCY 1 cut(s) 3568
Psp6I CCWGG 5 cut(s) 1438, 1578, 1998, 2944, 3223
PspCI CACGTG 1 cut(s) 2543
PspEI GGTNACC 1 cut(s) 1655
PspFI CCCAGC 1 cut(s) 1565
PspGI CCWGG 5 cut(s) 1438, 1578, 1998, 2944, 3223
PspPI GGNCC 7 cut(s) 22, 341, 466, 559, 2459, 3568, 4198
PspPPI RGGWCCY 1 cut(s) 3568
PspXI VCTCGAGB 1 cut(s) 1938
PstI CTGCAG 1 cut(s) 833
PstNI CAGNNNCTG 2 cut(s) 974, 2537
PsuI RGATCY 8 cut(s) 178, 251, 1045, 2587, 2737, 2795, 3313, 3539
PvuII CAGCTG 3 cut(s) 1205, 1669, 3626
RsaI GTAC 5 cut(s) 371, 648, 2052, 2934, 3773
RsaNI GTAC 5 cut(s) 370, 647, 2051, 2933, 3772
RseI CAYNNNNRTG 4 cut(s) 1488, 3361, 3846, 4050
SacI GAGCTC 1 cut(s) 935
Sau96I GGNCC 7 cut(s) 22, 341, 466, 559, 2459, 3568, 4198
SchI GAGTC 3 cut(s) 37, 983, 3674
ScrFI CCNGG 7 cut(s) 1015, 1440, 1580, 1737, 2000, 2946, 3225
SduI GDGCHC 8 cut(s) 935, 1225, 2764, 2898, 3417, 3754, 3945, 4104
SfcI CTRYAG 2 cut(s) 829, 1757
Sfr274I CTCGAG 3 cut(s) 1938, 2064, 2730
SinI GGWCC 3 cut(s) 341, 2459, 3568
SlaI CTCGAG 3 cut(s) 1938, 2064, 2730
SmiI ATTTAAAT 1 cut(s) 222
SmiMI CAYNNNNRTG 4 cut(s) 1488, 3361, 3846, 4050
SmlI CTYRAG 6 cut(s) 212, 1938, 2064, 2730, 3323, 3775
SmoI CTYRAG 6 cut(s) 212, 1938, 2064, 2730, 3323, 3775
SsiI CCGC 5 cut(s) 14, 2048, 2593, 2682, 3490
SspI AATATT 2 cut(s) 166, 2701
SstI GAGCTC 1 cut(s) 935
StyD4I CCNGG 7 cut(s) 1013, 1438, 1578, 1735, 1998, 2944, 3223
StyI CCWWGG 4 cut(s) 815, 1452, 1540, 2264
SwaI ATTTAAAT 1 cut(s) 222
TaiI ACGT 7 cut(s) 189, 2353, 2545, 3058, 3285, 3730, 4192
TaqII GACCGA 1 cut(s) 524
TatI WGTACW 2 cut(s) 369, 3771
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 9 cut(s) 353, 1805, 2426, 3073, 3080, 3481, 3514, 3818, 4159
TseFI GTSAC 5 cut(s) 857, 1430, 1574, 1885, 3805
Tsp45I GTSAC 5 cut(s) 857, 1430, 1574, 1885, 3805
TspGWI ACGGA 1 cut(s) 1121
Van91I CCANNNNNTGG 3 cut(s) 1256, 2414, 4207
VneI GTGCAC 1 cut(s) 3941
VpaK11BI GGWCC 3 cut(s) 341, 2459, 3568
VspI ATTAAT 1 cut(s) 3990
XagI CCTNNNNNAGG 2 cut(s) 3302, 3785
XapI RAATTY 9 cut(s) 217, 1064, 1318, 1424, 2070, 2195, 3035, 3093, 3908
XbaI TCTAGA 3 cut(s) 2386, 2605, 3784
XceI RCATGY 6 cut(s) 64, 645, 2233, 2501, 2987, 3364
XhoI CTCGAG 3 cut(s) 1938, 2064, 2730
XmaJI CCTAGG 1 cut(s) 2264
XmiI GTMKAC 1 cut(s) 2968
XmnI GAANNNNTTC 4 cut(s) 1171, 2208, 2273, 3911
Zsp2I ATGCAT 1 cut(s) 2989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.