pycom13g00580

Serine threonine-protein kinase sepA-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0006663
Physical Location & Seq
Reverse (-)
359171 .. 364559
5389 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g00580.5

Sequence Viewer

Length: 3021 bp
ATGGTGAGGTCAATGGATCGATCTCCTGAATTAACGACAAAAAATGTAAGTGCCAAACAAGGAGGAAAAGGTGTTGGTTATAGAGCATTCGGTTTCGGAACAAGAAATCAGGATGGTAGTTTTCAAAAGGCTGCCAAGATGCCAGTTTCTTTGGGAGGTAATGAACTCAGTAAATTTAGTGATACTCCAGGAGATGCTTCCTTGGATGATCTGTTTCATCCATTGGATAAGCATCCAGAGGATAGGGCAACTGAGGCTTCGACATCTGCATCTATGTCACATTCAAACCAAGGCAATACACCTGGGAATGATGCTGGGAAAGGTGACCTGGCTACAAAGTTGAGAGCTACAATTGCTCAAAAACAAATGGAGAGTGAAATGGGTCAGGCTAACGGCAGTGGTGGCAACCTGTTACAGCTTATGATGGGTGTTCTCAAAGATGATGTGATTGATATTGGTGGTTTGGTTTTCGACGAAAAGATGCCTGGAGAAAACCTATTTCCTCTACAGGCTGTTGAGTTCAGCCGGTTGGTTGGGTCATTGAGGCCAGATGAAACAGAAGATGTTATTGTCTCTGCCTGTCAGAAACTCATTGCTATCTTCCATCAGCGCCCAGAGCAGAAAATTGTTTTTGTTACACAGCATGGTTTGATCCCTCTAATGGAATTGCTTGAAGTACCTAAAACTCGCGTTATATGTTCTGTGCTTCAAATTATAAATCAGATAATCAAAGATAATACTGATTTCCAAGAAAATGCTTGTCTTGTGGGTCTGATCCCAGTGATAATGAGCTTTGCGGTTCCTAATCATTCCCGGGAAATTCGTATGGAAGCAGCTTATTTCTTACAGCAGCTTTGTCAGTCAAGCGCGCTGACATTGCAAATGTTCATAGCTTGCCGTGGAATACCTGTTTTGGTGGGCTTTTTAGAGGCTGACTATGCAAAATTCAGGGAAATGGTTCATCTAGCAATTGATGGTATGTGGCAAGTCTTTAAGCTTCAGCGGTCAACTCCTAGAAATGATTTTTGTCGCATAGCCGCAAAGAATGGAATACTGCTGAGGCTCATTAACACCCTTCATAGCTTGAATGAGGCAACCCGCCTAGCTTCCGTATCCGCTGGGGGTGGATTTCCACTAGAAGGTTCAGCTCAGCGCCCACGGTCTGGTTCATTAGATTCTGGTCATCCGATTTTTTCTCAGAGTGACACACCGCTTCCTACAACTGATCAACATGATCCCTCCAAGGTTAGACATGGAATGATTGATTTTCATTTGTCAGCTGGAACAGCAGAACCTGCACGTGCTTCAACTTCAAACTCTCAAAGATCAGATGCTGATCAATCAGATCCAAGATATCTTCATCTAGATACTGATAGAGCTCAATCTAGCAATGTAGTAGTGGAAGCTTCAGTTCCTTCTAAATTGACAGACTCATCATCAGTAGACAAAGTAGTAAATATTACAACCAAGGAACCTTCAACTACCTCTCGAGATCTAGACCTCAGGCAGCAGCGACCCGCTAATTCTTCTAGTAGGGCATCCACAGATAGACCTCCAAAAATGATGGAAGGTACATCGAACGGGTTTTCAACAACAGTAACTACTCAACAGGAGCAAGTTCGTCCCCTTCTTAGCTTGTTAGATAAAGAACCTCCTTCCAGACGCTTTTCTGGTCAGCTCGAGTATGTACGCCACCTGCCAGGTTTGGAGAGACATGAAAGTATATTGCCCCTTTTACATGCTTCTAATGAAAAGAAGACAAACGGAGAATTAGATTTCCTGATGGCCGAATTTGCCGACGTCTCTCAACGTGGTAGAGAAAATGGGAATGTTGATTCCACTGCTAGAGTTTCTCATAAAACGATAAATAAAGAGATGGGAACACTAGCTTCTATCAAAGGAGCTGCTTCCACCTCTGGCATAGCATCTCAGACAACATCAGGAGTACTTTCTGGTTCTGGAGTTCTAAATGCCAGACCAGGCAGTGCAACATCATCAGGTTTACTTTCCCACATGGTTTTAACATTGAATGCAGATGTTGCGAGGGAATACCTAGAAAAGGTGGCTGATCTTCTGCTTGAGTTTGCACAGGCAGATACTACTGTAAAGTCATACATGTGTAGCCAAAGTTTGCTCAGCCGTCTCTTCAAGATGTTCAATAGGGTGGAGCCCCCTATTCTTTTGAAGATACTGAAGTGTGTAAACCATTTGTCAACTGATCGAAACTGCTTAGAGAATCTTCAGCGTGCAGAAGCAATTAAATATTTGATTCCAAATCTTGAACTAAAAGAAGGAGCTCTTGTATCTCAAATACATCATGAGGTACTCAATGCTCTGTTCAATCTGTGCAAGATTAACAAGAGAAGACAGGAACAAGCAGCTGAGATTGGAATAATTCCACACTTAATGCACTTTATTGAGTCTAATTCTCCTTTGAAACAATATGCGTTGCCTTTACTGTGTGATATGGCTCATGCATCACGTAATTCAAGGGAGCAACTAAGGGCTCATGGTGGGTTGGATGTTTACTTAAGCCTTCTCGAGAATGAACTTTGGTCTGTGACTGCATTGGATTCGATTGCTGTGTGCTTGGCTCATGACAATGACAACTGGAAGGTGGAACAAGCATTATTGAAAAAGGATGCAGTTCAGAAATTGGTGAAATTTTTCCAGTGCTGTCCAGAACAGTATTTCGTGCACATATTGGAGCCATTTTTGAAAATTATCACGAAATCATCGCGAATTAATACAACGTTAGCTGTTAATGGTTTGACACCACTGCTTATCACAAGACTGGACCATCAGGATGCTATTGCCCGTCTAAATCTTCTTAAACTGATAAAGGCTGTTTATGAGCACCACCCGCGGCCAAAGCAATTGATTGTGGAGAATGATCGACCTCAGAAGCTTCAAAATCTAATAGAAGAACGCAGAGATGGGCAACGAGTGGGTGGCCAAGTGTTGGTAAAACAAATGGCTACTTCGCTGCTTAAAGCACTACATATTAACACCGTCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000278 GO:0000281 GO:0000910 GO:0001558 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005813 GO:0005815 GO:0005829 GO:0005856 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007049 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0008361 GO:0009893 GO:0009987 GO:0010324 GO:0010562 GO:0010604 GO:0015630 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022402 GO:0022603 GO:0022604 GO:0023014 GO:0023052 GO:0030587 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032147 GO:0032268 GO:0032270 GO:0032502 GO:0032506 GO:0032535 GO:0033554 GO:0033674 GO:0035556 GO:0036089 GO:0036211 GO:0040008 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0045995 GO:0046777 GO:0048518 GO:0048522 GO:0048638 GO:0048856 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051246 GO:0051247 GO:0051301 GO:0051302 GO:0051338 GO:0051347 GO:0051510 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0061024 GO:0061387 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090066 GO:0090702 GO:0099024 GO:0099120 GO:0140096 GO:1901564 GO:1903047 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1007

Amino Acids

110.89

Weight (kDa)

7.4

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 716
AarI CACCTGC 1 cut(s) 1704
AatII GACGTC 1 cut(s) 1803
Acc36I ACCTGC 2 cut(s) 1303, 1704
AccB7I CCANNNNNTGG 2 cut(s) 1163, 2962
AccI GTMKAC 1 cut(s) 1443
AccII CGCG 4 cut(s) 690, 869, 2740, 2866
AciI CCGC 9 cut(s) 797, 1003, 1038, 1099, 1116, 1211, 1518, 2864, 2866
AclI AACGTT 1 cut(s) 2753
AclWI GGATC 5 cut(s) 24, 646, 769, 1229, 1340
AcoI YGGCCR 3 cut(s) 1785, 2867, 2953
AcsI RAATTY 5 cut(s) 173, 819, 944, 1790, 2663
AcuI CTGAAG 4 cut(s) 983, 1392, 2213, 2225
AcvI CACGTG 1 cut(s) 1301
AcyI GRCGYC 1 cut(s) 1800
AfaI GTAC 5 cut(s) 678, 1573, 1689, 1947, 2325
AfiI CCNNNNNNNGG 5 cut(s) 661, 1139, 1163, 2059, 2962
AflII CTTAAG 1 cut(s) 2530
AflIII ACRYGT 1 cut(s) 2115
AjnI CCWGG 6 cut(s) 187, 301, 327, 484, 1699, 1978
Alw21I GWGCWC 4 cut(s) 1381, 2299, 2700, 2859
Alw26I GTCTC 4 cut(s) 577, 1705, 1807, 2147
Alw44I GTGCAC 1 cut(s) 2696
AlwI GGATC 5 cut(s) 24, 646, 769, 1229, 1340
AlwNI CAGNNNCTG 2 cut(s) 1295, 1334
Ama87I CYCGRG 4 cut(s) 813, 1488, 1679, 2540
AoxI GGCC 4 cut(s) 545, 1785, 2867, 2953
ApaLI GTGCAC 1 cut(s) 2696
ApeKI GCWGC 8 cut(s) 131, 833, 850, 1507, 1510, 1904, 2378, 2986
ApoI RAATTY 5 cut(s) 173, 819, 944, 1790, 2663
AseI ATTAAT 1 cut(s) 2745
Asp700I GAANNNNTTC 2 cut(s) 957, 2666
AspLEI GCGC 4 cut(s) 612, 869, 871, 1155
AspS9I GGNCC 1 cut(s) 2797
AsuC2I CCSGG 2 cut(s) 814, 815
AsuHPI GGTGA 3 cut(s) 16, 335, 2671
AvaI CYCGRG 4 cut(s) 813, 1488, 1679, 2540
AvaII GGWCC 1 cut(s) 2797
AxyI CCTNAGG 1 cut(s) 1502
BaeGI GKGCMC 1 cut(s) 2700
BaeI ACNNNNGTAYC 4 cut(s) 2088, 2121, 2180, 2213
BalI TGGCCA 1 cut(s) 2955
BanII GRGCYC 4 cut(s) 1381, 2172, 2299, 2509
BbrPI CACGTG 1 cut(s) 1301
BbsI GAAGAC 2 cut(s) 1763, 2371
Bbv12I GWGCWC 4 cut(s) 1381, 2299, 2700, 2859
BbvCI CCTCAGC 1 cut(s) 1058
BbvI GCAGC 8 cut(s) 118, 845, 862, 1519, 1522, 1891, 2390, 2973
BccI CCATC 9 cut(s) 107, 418, 612, 968, 1558, 1777, 1870, 2808, 2930
BceAI ACGGC 3 cut(s) 409, 882, 2124
BcgI CGANNNNNNTGC 2 cut(s) 2556, 2590
BciT130I CCWGG 6 cut(s) 189, 303, 329, 486, 1701, 1980
BciVI GTATCC 1 cut(s) 1123
BclI TGATCA 2 cut(s) 1225, 1336
BcnI CCSGG 2 cut(s) 814, 815
BcoDI GTCTC 4 cut(s) 577, 1705, 1807, 2147
BfmI CTRYAG 1 cut(s) 506
BfoI RGCGCY 2 cut(s) 613, 1156
BfrI CTTAAG 1 cut(s) 2530
BfuAI ACCTGC 2 cut(s) 1303, 1704
BfuI GTATCC 1 cut(s) 1123
BglII AGATCT 1 cut(s) 1492
BlpI GCTNAGC 2 cut(s) 1149, 2135
BmcAI AGTACT 1 cut(s) 1947
Bme1390I CCNGG 8 cut(s) 189, 303, 329, 486, 814, 815, 1701, 1980
Bme18I GGWCC 1 cut(s) 2797
BmeT110I CYCGRG 4 cut(s) 813, 1488, 1679, 2540
BmgT120I GGNCC 1 cut(s) 2797
BmiI GGNNCC 4 cut(s) 801, 1473, 2169, 2709
BmrFI CCNGG 8 cut(s) 189, 303, 329, 486, 814, 815, 1701, 1980
BmrI ACTGGG 1 cut(s) 773
BmuI ACTGGG 1 cut(s) 773
BpiI GAAGAC 2 cut(s) 1763, 2371
BpmI CTGGAG 3 cut(s) 171, 507, 1980
Bpu10I CCTNAGC 1 cut(s) 1058
Bpu1102I GCTNAGC 2 cut(s) 1149, 2135
BpuEI CTTGAG 1 cut(s) 2099
BpuMI CCSGG 2 cut(s) 814, 815
Bsa29I ATCGAT 1 cut(s) 19
BsaAI YACGTR 2 cut(s) 1301, 2483
BsaBI GATNNNNATC 2 cut(s) 231, 1335
BsaHI GRCGYC 1 cut(s) 1800
BsaJI CCNNGG 9 cut(s) 201, 289, 302, 813, 898, 1157, 1242, 1467, 2864
BsaXI ACNNNNNCTCC 2 cut(s) 2286, 2316
Bsc4I CCNNNNNNNGG 5 cut(s) 661, 1139, 1163, 2059, 2962
Bse118I RCCGGY 1 cut(s) 525
Bse1I ACTGG 5 cut(s) 143, 779, 2615, 2671, 2799
Bse21I CCTNAGG 1 cut(s) 1502
Bse3DI GCAATG 3 cut(s) 591, 875, 1396
Bse8I GATNNNNATC 2 cut(s) 231, 1335
BseBI CCWGG 6 cut(s) 189, 303, 329, 486, 1701, 1980
BseCI ATCGAT 1 cut(s) 19
BseDI CCNNGG 9 cut(s) 201, 289, 302, 813, 898, 1157, 1242, 1467, 2864
BseGI GGATG 9 cut(s) 118, 211, 217, 232, 1183, 1538, 2527, 2647, 2812
BseJI GATNNNNATC 2 cut(s) 231, 1335
BseLI CCNNNNNNNGG 5 cut(s) 661, 1139, 1163, 2059, 2962
BseMI GCAATG 3 cut(s) 591, 875, 1396
BseNI ACTGG 5 cut(s) 143, 779, 2615, 2671, 2799
BsePI GCGCGC 1 cut(s) 867
BseSI GKGCMC 1 cut(s) 2700
BseXI GCAGC 8 cut(s) 118, 845, 862, 1519, 1522, 1891, 2390, 2973
BseYI CCCAGC 2 cut(s) 314, 1118
BsgI GTGCAG 2 cut(s) 1281, 2268
Bsh1236I CGCG 4 cut(s) 690, 869, 2740, 2866
BshFI GGCC 4 cut(s) 547, 1787, 2869, 2955
BshVI ATCGAT 1 cut(s) 19
BsiHKAI GWGCWC 4 cut(s) 1381, 2299, 2700, 2859
BsiHKCI CYCGRG 4 cut(s) 813, 1488, 1679, 2540
BsiSI CCGG 2 cut(s) 526, 814
BslFI GGGAC 1 cut(s) 1608
BslI CCNNNNNNNGG 5 cut(s) 661, 1139, 1163, 2059, 2962
BsmAI GTCTC 4 cut(s) 577, 1705, 1807, 2147
BsmBI CGTCTC 2 cut(s) 1807, 2147
BsmFI GGGAC 1 cut(s) 1608
BsmI GAATGC 2 cut(s) 86, 2035
BsnI GGCC 4 cut(s) 547, 1787, 2869, 2955
BsoBI CYCGRG 4 cut(s) 813, 1488, 1679, 2540
Bsp1286I GDGCHC 6 cut(s) 1381, 2172, 2299, 2509, 2700, 2859
Bsp1720I GCTNAGC 2 cut(s) 1149, 2135
Bsp68I TCGCGA 1 cut(s) 2740
BspACI CCGC 9 cut(s) 797, 1003, 1038, 1099, 1116, 1211, 1518, 2864, 2866
BspANI GGCC 4 cut(s) 547, 1787, 2869, 2955
BspDI ATCGAT 1 cut(s) 19
BspFNI CGCG 4 cut(s) 690, 869, 2740, 2866
BspHI TCATGA 2 cut(s) 2317, 2596
BspLI GGNNCC 4 cut(s) 801, 1473, 2169, 2709
BspMI ACCTGC 2 cut(s) 1303, 1704
BspPI GGATC 5 cut(s) 24, 646, 769, 1229, 1340
BspTI CTTAAG 1 cut(s) 2530
BsrDI GCAATG 3 cut(s) 591, 875, 1396
BsrFI RCCGGY 1 cut(s) 525
BsrI ACTGG 5 cut(s) 143, 779, 2615, 2671, 2799
BssAI RCCGGY 1 cut(s) 525
BssECI CCNNGG 9 cut(s) 201, 289, 302, 813, 898, 1157, 1242, 1467, 2864
BssHII GCGCGC 1 cut(s) 867
BssNI GRCGYC 1 cut(s) 1800
BssT1I CCWWGG 4 cut(s) 201, 289, 1242, 1467
Bst2UI CCWGG 6 cut(s) 189, 303, 329, 486, 1701, 1980
Bst4CI ACNGT 6 cut(s) 1161, 1597, 2104, 2460, 2688, 3013
Bst6I CTCTTC 1 cut(s) 2150
BstACI GRCGYC 1 cut(s) 1800
BstAFI CTTAAG 1 cut(s) 2530
BstAPI GCANNNNNTGC 2 cut(s) 1295, 2039
BstBAI YACGTR 2 cut(s) 1301, 2483
BstC8I GCNNGC 3 cut(s) 869, 895, 2247
BstDSI CCRYGG 3 cut(s) 898, 1157, 2864
BstEII GGTNACC 1 cut(s) 323
BstENI CCTNNNNNAGG 1 cut(s) 2057
BstF5I GGATG 9 cut(s) 118, 211, 217, 232, 1183, 1538, 2527, 2647, 2812
BstFNI CGCG 4 cut(s) 690, 869, 2740, 2866
BstH2I RGCGCY 2 cut(s) 613, 1156
BstHHI GCGC 4 cut(s) 612, 869, 871, 1155
BstMAI GTCTC 4 cut(s) 577, 1705, 1807, 2147
BstNI CCWGG 6 cut(s) 189, 303, 329, 486, 1701, 1980
BstNSI RCATGY 2 cut(s) 1742, 2119
BstPI GGTNACC 1 cut(s) 323
BstSCI CCNGG 8 cut(s) 187, 301, 327, 484, 812, 813, 1699, 1978
BstSFI CTRYAG 1 cut(s) 506
BstSLI GKGCMC 1 cut(s) 2700
BstUI CGCG 4 cut(s) 690, 869, 2740, 2866
BstV1I GCAGC 8 cut(s) 118, 845, 862, 1519, 1522, 1891, 2390, 2973
BstV2I GAAGAC 2 cut(s) 1763, 2371
BstX2I RGATCY 2 cut(s) 1345, 1492
BstYI RGATCY 2 cut(s) 1345, 1492
Bsu15I ATCGAT 1 cut(s) 19
Bsu36I CCTNAGG 1 cut(s) 1502
BsuI GTATCC 1 cut(s) 1123
BsuRI GGCC 4 cut(s) 547, 1787, 2869, 2955
BsuTUI ATCGAT 1 cut(s) 19
BtgI CCRYGG 3 cut(s) 898, 1157, 2864
BtgZI GCGATG 1 cut(s) 2721
BtsCI GGATG 9 cut(s) 118, 211, 217, 232, 1183, 1538, 2527, 2647, 2812
BtsI GCAGTG 4 cut(s) 403, 1839, 1990, 2777
BtsIMutI CAGTG 6 cut(s) 403, 786, 1839, 1990, 2678, 2777
BtuMI TCGCGA 1 cut(s) 2740
BveI ACCTGC 2 cut(s) 1303, 1704
Cac8I GCNNGC 3 cut(s) 869, 895, 2247
CaiI CAGNNNCTG 2 cut(s) 1295, 1334
CciI TCATGA 2 cut(s) 2317, 2596
CfoI GCGC 4 cut(s) 612, 869, 871, 1155
Cfr10I RCCGGY 1 cut(s) 525
Cfr13I GGNCC 1 cut(s) 2797
Cfr42I CCGCGG 1 cut(s) 2867
Cfr9I CCCGGG 1 cut(s) 813
ClaI ATCGAT 1 cut(s) 19
CseI GACGC 1 cut(s) 1671
Csp6I GTAC 5 cut(s) 677, 1572, 1688, 1946, 2324
CviQI GTAC 5 cut(s) 677, 1572, 1688, 1946, 2324
EaeI YGGCCR 3 cut(s) 1785, 2867, 2953
Eam1104I CTCTTC 1 cut(s) 2150
EarI CTCTTC 1 cut(s) 2150
Ecl136II GAGCTC 2 cut(s) 1379, 2297
Eco130I CCWWGG 4 cut(s) 201, 289, 1242, 1467
Eco24I GRGCYC 4 cut(s) 1381, 2172, 2299, 2509
Eco32I GATATC 1 cut(s) 1355
Eco47I GGWCC 1 cut(s) 2797
Eco53kI GAGCTC 2 cut(s) 1379, 2297
Eco57I CTGAAG 4 cut(s) 983, 1392, 2213, 2225
Eco72I CACGTG 1 cut(s) 1301
Eco81I CCTNAGG 1 cut(s) 1502
Eco88I CYCGRG 4 cut(s) 813, 1488, 1679, 2540
Eco91I GGTNACC 1 cut(s) 323
EcoICRI GAGCTC 2 cut(s) 1379, 2297
EcoNI CCTNNNNNAGG 1 cut(s) 2057
EcoO65I GGTNACC 1 cut(s) 323
EcoRII CCWGG 6 cut(s) 187, 301, 327, 484, 1699, 1978
EcoRV GATATC 1 cut(s) 1355
EcoT14I CCWWGG 4 cut(s) 201, 289, 1242, 1467
EcoT22I ATGCAT 1 cut(s) 2479
EcoT38I GRGCYC 4 cut(s) 1381, 2172, 2299, 2509
ErhI CCWWGG 4 cut(s) 201, 289, 1242, 1467
Esp3I CGTCTC 2 cut(s) 1807, 2147
FalI AAGNNNNNCTT 2 cut(s) 2283, 2315
FaqI GGGAC 1 cut(s) 1608
FauI CCCGC 3 cut(s) 1106, 1525, 2871
FbaI TGATCA 2 cut(s) 1225, 1336
FblI GTMKAC 1 cut(s) 1443
FokI GGATG 9 cut(s) 125, 204, 218, 219, 1170, 1525, 2534, 2654, 2819
FriOI GRGCYC 4 cut(s) 1381, 2172, 2299, 2509
GlaI GCGC 4 cut(s) 611, 868, 870, 1154
GsaI CCCAGC 2 cut(s) 318, 1122
GsuI CTGGAG 3 cut(s) 171, 507, 1980
HaeII RGCGCY 2 cut(s) 613, 1156
HaeIII GGCC 4 cut(s) 547, 1787, 2869, 2955
HapII CCGG 2 cut(s) 526, 814
HgaI GACGC 1 cut(s) 1671
HhaI GCGC 4 cut(s) 612, 869, 871, 1155
Hin1I GRCGYC 1 cut(s) 1800
Hin6I GCGC 4 cut(s) 610, 867, 869, 1153
HinP1I GCGC 4 cut(s) 610, 867, 869, 1153
HincII GTYRAC 2 cut(s) 1008, 2214
HindII GTYRAC 2 cut(s) 1008, 2214
HindIII AAGCTT 3 cut(s) 995, 1404, 2906
HinfI GANTC 7 cut(s) 1175, 1430, 1835, 2236, 2269, 2420, 2573
HpaII CCGG 2 cut(s) 526, 814
HphI GGTGA 3 cut(s) 16, 335, 2671
Hpy166II GTNNAC 7 cut(s) 1008, 1444, 2003, 2203, 2214, 2527, 2698
Hpy8I GTNNAC 7 cut(s) 1008, 1444, 2003, 2203, 2214, 2527, 2698
Hpy99I CGWCG 2 cut(s) 476, 1802
HpyCH4III ACNGT 6 cut(s) 1161, 1597, 2104, 2460, 2688, 3013
HpyCH4IV ACGT 5 cut(s) 1300, 1800, 1810, 2482, 2753
HpySE526I ACGT 5 cut(s) 1300, 1800, 1810, 2482, 2753
Hsp92I GRCGYC 1 cut(s) 1800
HspAI GCGC 4 cut(s) 610, 867, 869, 1153
Ksp22I TGATCA 2 cut(s) 1225, 1336
KspI CCGCGG 1 cut(s) 2867
LmnI GCTCC 6 cut(s) 1612, 1901, 2167, 2294, 2494, 2707
Lsp1109I GCAGC 8 cut(s) 118, 845, 862, 1519, 1522, 1891, 2390, 2973
MaeII ACGT 5 cut(s) 1300, 1800, 1810, 2482, 2753
MaeIII GTNAC 7 cut(s) 276, 323, 411, 634, 1202, 1597, 2560
MfeI CAATTG 3 cut(s) 351, 969, 2876
MflI RGATCY 2 cut(s) 1345, 1492
MhlI GDGCHC 6 cut(s) 1381, 2172, 2299, 2509, 2700, 2859
MlsI TGGCCA 1 cut(s) 2955
MluNI TGGCCA 1 cut(s) 2955
MlyI GAGTC 2 cut(s) 1424, 2429
MmeI TCCRAC 1 cut(s) 2499
Mox20I TGGCCA 1 cut(s) 2955
Mph1103I ATGCAT 1 cut(s) 2479
MroXI GAANNNNTTC 2 cut(s) 957, 2666
MscI TGGCCA 1 cut(s) 2955
MslI CAYNNNNRTG 3 cut(s) 2116, 2601, 2805
Msp20I TGGCCA 1 cut(s) 2955
MspA1I CMGCKG 5 cut(s) 1003, 1118, 1280, 2381, 2866
MspCI CTTAAG 1 cut(s) 2530
MspI CCGG 2 cut(s) 526, 814
MspR9I CCNGG 8 cut(s) 189, 303, 329, 486, 814, 815, 1701, 1980
MunI CAATTG 3 cut(s) 351, 969, 2876
Mva1269I GAATGC 2 cut(s) 86, 2035
MvaI CCWGG 6 cut(s) 189, 303, 329, 486, 1701, 1980
MvnI CGCG 4 cut(s) 690, 869, 2740, 2866
NciI CCSGG 2 cut(s) 814, 815
NlaIV GGNNCC 4 cut(s) 801, 1473, 2169, 2709
NmuCI GTSAC 4 cut(s) 276, 323, 1202, 2560
NruI TCGCGA 1 cut(s) 2740
NsiI ATGCAT 1 cut(s) 2479
NspI RCATGY 2 cut(s) 1742, 2119
PaeR7I CTCGAG 3 cut(s) 1488, 1679, 2540
PagI TCATGA 2 cut(s) 2317, 2596
PaqCI CACCTGC 1 cut(s) 1704
PauI GCGCGC 1 cut(s) 867
PciI ACATGT 1 cut(s) 2115
PctI GAATGC 2 cut(s) 86, 2035
PdmI GAANNNNTTC 2 cut(s) 957, 2666
PfeI GAWTC 5 cut(s) 1175, 1835, 2236, 2269, 2573
PflMI CCANNNNNTGG 2 cut(s) 1163, 2962
PfoI TCCNGGA 1 cut(s) 187
PleI GAGTC 2 cut(s) 1424, 2428
PmaCI CACGTG 1 cut(s) 1301
PmlI CACGTG 1 cut(s) 1301
PpsI GAGTC 2 cut(s) 1424, 2428
Ppu21I YACGTR 2 cut(s) 1301, 2483
PscI ACATGT 1 cut(s) 2115
PshBI ATTAAT 1 cut(s) 2745
PsiI TTATAA 1 cut(s) 716
Psp124BI GAGCTC 2 cut(s) 1381, 2299
Psp1406I AACGTT 1 cut(s) 2753
Psp6I CCWGG 6 cut(s) 187, 301, 327, 484, 1699, 1978
PspCI CACGTG 1 cut(s) 1301
PspEI GGTNACC 1 cut(s) 323
PspFI CCCAGC 2 cut(s) 314, 1118
PspGI CCWGG 6 cut(s) 187, 301, 327, 484, 1699, 1978
PspN4I GGNNCC 4 cut(s) 801, 1473, 2169, 2709
PspPI GGNCC 1 cut(s) 2797
PspXI VCTCGAGB 1 cut(s) 1679
PstNI CAGNNNCTG 2 cut(s) 1295, 1334
PsuI RGATCY 2 cut(s) 1345, 1492
PteI GCGCGC 1 cut(s) 867
PvuII CAGCTG 2 cut(s) 1280, 2381
RruI TCGCGA 1 cut(s) 2740
RsaI GTAC 5 cut(s) 678, 1573, 1689, 1947, 2325
RsaNI GTAC 5 cut(s) 677, 1572, 1688, 1946, 2324
RseI CAYNNNNRTG 3 cut(s) 2116, 2601, 2805
SacI GAGCTC 2 cut(s) 1381, 2299
SacII CCGCGG 1 cut(s) 2867
Sau96I GGNCC 1 cut(s) 2797
ScaI AGTACT 1 cut(s) 1947
SchI GAGTC 2 cut(s) 1424, 2429
ScrFI CCNGG 8 cut(s) 189, 303, 329, 486, 814, 815, 1701, 1980
SduI GDGCHC 6 cut(s) 1381, 2172, 2299, 2509, 2700, 2859
SfcI CTRYAG 1 cut(s) 506
Sfr274I CTCGAG 3 cut(s) 1488, 1679, 2540
Sfr303I CCGCGG 1 cut(s) 2867
SgrBI CCGCGG 1 cut(s) 2867
SinI GGWCC 1 cut(s) 2797
SlaI CTCGAG 3 cut(s) 1488, 1679, 2540
SmaI CCCGGG 1 cut(s) 815
SmiMI CAYNNNNRTG 3 cut(s) 2116, 2601, 2805
SmlI CTYRAG 5 cut(s) 1488, 1679, 2078, 2530, 2540
SmoI CTYRAG 5 cut(s) 1488, 1679, 2078, 2530, 2540
SsiI CCGC 9 cut(s) 797, 1003, 1038, 1099, 1116, 1211, 1518, 2864, 2866
SspI AATATT 2 cut(s) 1459, 2264
SstI GAGCTC 2 cut(s) 1381, 2299
StyD4I CCNGG 8 cut(s) 187, 301, 327, 484, 812, 813, 1699, 1978
StyI CCWWGG 4 cut(s) 201, 289, 1242, 1467
TaaI ACNGT 6 cut(s) 1161, 1597, 2104, 2460, 2688, 3013
TaiI ACGT 5 cut(s) 1303, 1803, 1813, 2485, 2756
TatI WGTACW 1 cut(s) 1945
TauI GCSGC 2 cut(s) 1040, 2869
TfiI GAWTC 5 cut(s) 1175, 1835, 2236, 2269, 2573
TscAI CASTG 6 cut(s) 403, 786, 1846, 1990, 2678, 2784
TseFI GTSAC 4 cut(s) 276, 323, 1202, 2560
TseI GCWGC 8 cut(s) 131, 833, 850, 1507, 1510, 1904, 2378, 2986
Tsp45I GTSAC 4 cut(s) 276, 323, 1202, 2560
TspGWI ACGGA 2 cut(s) 1099, 1779
TspMI CCCGGG 1 cut(s) 813
TspRI CASTG 6 cut(s) 403, 786, 1846, 1990, 2678, 2784
Van91I CCANNNNNTGG 2 cut(s) 1163, 2962
Vha464I CTTAAG 1 cut(s) 2530
VneI GTGCAC 1 cut(s) 2696
VpaK11BI GGWCC 1 cut(s) 2797
VspI ATTAAT 1 cut(s) 2745
XagI CCTNNNNNAGG 1 cut(s) 2057
XapI RAATTY 5 cut(s) 173, 819, 944, 1790, 2663
XbaI TCTAGA 2 cut(s) 1363, 1495
XceI RCATGY 2 cut(s) 1742, 2119
XhoI CTCGAG 3 cut(s) 1488, 1679, 2540
XmaI CCCGGG 1 cut(s) 813
XmiI GTMKAC 1 cut(s) 1443
XmnI GAANNNNTTC 2 cut(s) 957, 2666
ZraI GACGTC 1 cut(s) 1801
ZrmI AGTACT 1 cut(s) 1947
Zsp2I ATGCAT 1 cut(s) 2479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.