FvH4_4g37160

Leucine Rich repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015952
Physical Location & Seq
Forward (+)
33747556 .. 33749416
1861 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g37160.t1

Sequence Viewer

Length: 1230 bp
ATGGAGAGCATGGGCAGCATCAACGAGACACTGAGAGATGACGAGCTACGAGCGGTGCTGGGAAAGCTGGACAGCCAGAGGGACAAAGAGGTGTTCGGGTTGGTGTGCAAGAGGTGGCTTCACTTGCAGAGCACCGAGAGGAGGAGGCTCTCCGCACGTGCGGGGCCTCACATGCTCCGCAAATTGGCCGCCCGGTTCTCTCGCCTTCACGAGCTCGACCTCTCCCAGTCCCTCTCCAGGTCCTTCTATCCTGGTGTCACCGACTCTGACCTCTCCGTCATTGCCAATGGCTTCAAGTCCCTCAGGCTCCTCTCCCTCCAAAACTGTAAAGGAGTTACTGATGCTGGAATCATTGCAATTGGATCTGGTCTTTCGTCACTACAGTCTCTAGATGTATCATATTGCAGAAAGTTAACAGACAAAGGATTATCAGCTGTTGCTCAGGGCTGCTCTGGCCTTCGGGCACTGCATCTTGCTGGCTGTAGATTTATAACCGATGTGTTATTACATGCTCTTTCATTGAATTGTCACTCTTTACGGCACTTGGGTCTGCAGGGCTGTACTAATATATCTGATTCTGGACTTGCTGATCTTGTAAATGGATGTCATCAGATCGAGTTTTTAGACATGAACAAATGCAGCAACATCGGAGACATTGGCGTTTCCAGTGTCTCCAAGGCATGTTCATCTTCTCTCAAGACACTCAAGCTATTGGACTGTTACAAAGTCGGGGATGAGTCTATAATATCCTTGGCCAAATTCTGCCAAAATCTTGAAACCTTAATCATTGGTGGCTGCCGTGACATATCTGATGCATCAGTGAAATTGCTGGCCTCTTCTTGTAAGAGTAGTCTCAAGAACTTGCGGATGGATTGGTGTTTAAATATCACAGACTCTTGTTTGAGCTGCATTCTGGTTGAGTGCAGAAATCTTGAGGCTCTTGACATTGGGTGCTGCGAGGAGGTGTCAGATGCTGCCTTTCAGGGCTTAAACGGTGGTGAAGTCGAGCTGACCCTGACCTTAAAGGTCTTAAAGGTTAGCAACTGCCCGAAAATCAGTGTGGCAGGCATAGCTATGGTTTTGGAGAAATGCGAGATGTTAGAATACTTGGATGTAAGGTCATGCCCACAAATTACGAAGGCGGGTTGTGATGAGGCCGGATTGCAGTTTCCTCAATCTTGTAAAGTCAACTTCACCGGGAGTTTAAGGGAGCCTGATGTCTTACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_6

Protein Analysis

410

Amino Acids

44.28

Weight (kDa)

6.69

Isoelectric Point (pI)

50.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 65 - 192 9.6e-11 Leucine Rich Repeat Domain of unknown function (DUF7885)
LRR_At5g56370 PF24758 125 - 237 8.9e-09 FBD-associated F-box protein At5g56370, LRR repeats
DUF7885 PF25372 175 - 387 1.9e-16 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015952)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23340
fragaria_vesca FvH4_4g37160
prunus_persica Prupe.1G349400_v2.0.a1
pyrus_communis pycom13g00250
rosa_chinensis RchiOBHm_Chr4g0446961
rosa_laevigata RLG00000005617
rosa_multiflora Rmu_sc0001753.1_g000005 Rmu_sc0011539.1_g000001
rosa_roxburghii Rroxscaffold_5G00387290
rosa_rugosa Rorug04G0376500
rosa_samantha Rh4AG438000 Rh4BG446900 Rh4CG464200 Rh4DG445700
rosa_wichuraiana Rw4G037460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 491
AasI GACNNNNNNGTC 1 cut(s) 275
AccBSI CCGCTC 1 cut(s) 53
AciI CCGC 7 cut(s) 53, 153, 161, 178, 189, 865, 1142
AclWI GGATC 1 cut(s) 370
AcoI YGGCCR 2 cut(s) 186, 753
AcsI RAATTY 1 cut(s) 758
AcvI CACGTG 1 cut(s) 158
AfaI GTAC 1 cut(s) 562
AfiI CCNNNNNNNGG 3 cut(s) 141, 184, 237
AgsI TTSAA 3 cut(s) 295, 523, 776
AjnI CCWGG 2 cut(s) 236, 250
AluBI AGCT 8 cut(s) 46, 67, 214, 434, 709, 906, 1009, 1073
AluI AGCT 8 cut(s) 46, 67, 214, 434, 709, 906, 1009, 1073
Alw21I GWGCWC 2 cut(s) 134, 216
Alw26I GTCTC 5 cut(s) 20, 390, 645, 676, 857
AlwI GGATC 1 cut(s) 370
AlwNI CAGNNNCTG 1 cut(s) 974
AoxI GGCC 6 cut(s) 164, 186, 454, 753, 831, 1155
ApeKI GCWGC 7 cut(s) 15, 447, 639, 795, 906, 954, 974
ApoI RAATTY 1 cut(s) 758
AspS9I GGNCC 2 cut(s) 164, 240
AsuC2I CCSGG 2 cut(s) 193, 1198
AsuHPI GGTGA 3 cut(s) 250, 1010, 1186
AvaII GGWCC 1 cut(s) 240
AxyI CCTNAGG 1 cut(s) 302
BaeGI GKGCMC 1 cut(s) 466
BalI TGGCCA 1 cut(s) 755
BanII GRGCYC 1 cut(s) 216
BauI CACGAG 1 cut(s) 209
BbrPI CACGTG 1 cut(s) 158
Bbv12I GWGCWC 2 cut(s) 134, 216
BbvI GCAGC 7 cut(s) 27, 434, 651, 782, 893, 941, 961
BccI CCATC 1 cut(s) 862
BceAI ACGGC 2 cut(s) 554, 783
BcgI CGANNNNNNTGC 2 cut(s) 628, 662
BciT130I CCWGG 2 cut(s) 238, 252
BcnI CCSGG 2 cut(s) 193, 1198
BcoDI GTCTC 5 cut(s) 20, 390, 645, 676, 857
BfaI CTAG 1 cut(s) 389
BfmI CTRYAG 3 cut(s) 380, 481, 551
BisI GCNGC 8 cut(s) 16, 189, 448, 640, 796, 907, 955, 975
BlsI GCNGC 8 cut(s) 17, 190, 449, 641, 797, 908, 956, 976
Bme1390I CCNGG 4 cut(s) 193, 238, 252, 1198
Bme18I GGWCC 1 cut(s) 240
BmgT120I GGNCC 2 cut(s) 164, 240
BmiI GGNNCC 3 cut(s) 165, 308, 1212
BmrFI CCNGG 4 cut(s) 193, 238, 252, 1198
BmrI ACTGGG 1 cut(s) 220
BmsI GCATC 6 cut(s) 27, 331, 478, 802, 824, 961
BmuI ACTGGG 1 cut(s) 220
BpmI CTGGAG 1 cut(s) 220
Bpu10I CCTNAGC 1 cut(s) 441
BpuEI CTTGAG 4 cut(s) 680, 689, 839, 953
BpuMI CCSGG 2 cut(s) 193, 1198
BsaAI YACGTR 1 cut(s) 158
BsaJI CCNNGG 2 cut(s) 675, 750
Bsc4I CCNNNNNNNGG 3 cut(s) 141, 184, 237
Bse1I ACTGG 2 cut(s) 226, 666
Bse21I CCTNAGG 1 cut(s) 302
Bse3DI GCAATG 2 cut(s) 279, 351
BseBI CCWGG 2 cut(s) 238, 252
BseDI CCNNGG 2 cut(s) 675, 750
BseGI GGATG 4 cut(s) 608, 739, 873, 1117
BseLI CCNNNNNNNGG 3 cut(s) 141, 184, 237
BseMI GCAATG 2 cut(s) 279, 351
BseMII CTCAG 3 cut(s) 23, 316, 455
BseNI ACTGG 2 cut(s) 226, 666
BseRI GAGGAG 4 cut(s) 154, 157, 299, 974
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 7 cut(s) 27, 434, 651, 782, 893, 941, 961
BseYI CCCAGC 1 cut(s) 58
BsgI GTGCAG 1 cut(s) 943
BshFI GGCC 6 cut(s) 166, 188, 456, 755, 833, 1157
BsiHKAI GWGCWC 2 cut(s) 134, 216
BsiSI CCGG 3 cut(s) 193, 1158, 1197
BslFI GGGAC 3 cut(s) 95, 214, 283
BslI CCNNNNNNNGG 3 cut(s) 141, 184, 237
BsmAI GTCTC 5 cut(s) 20, 390, 645, 676, 857
BsmFI GGGAC 3 cut(s) 95, 214, 283
BsmI GAATGC 1 cut(s) 909
BsnI GGCC 6 cut(s) 166, 188, 456, 755, 833, 1157
Bsp1286I GDGCHC 3 cut(s) 134, 216, 466
Bsp143I GATC 3 cut(s) 362, 589, 612
BspACI CCGC 7 cut(s) 53, 153, 161, 178, 189, 865, 1142
BspANI GGCC 6 cut(s) 166, 188, 456, 755, 833, 1157
BspCNI CTCAG 3 cut(s) 24, 315, 454
BspLI GGNNCC 3 cut(s) 165, 308, 1212
BspMAI CTGCAG 1 cut(s) 555
BspPI GGATC 1 cut(s) 370
BsrBI CCGCTC 1 cut(s) 53
BsrDI GCAATG 2 cut(s) 279, 351
BsrI ACTGG 2 cut(s) 226, 666
BssECI CCNNGG 2 cut(s) 675, 750
BssMI GATC 3 cut(s) 362, 589, 612
BssSI CACGAG 1 cut(s) 209
BssT1I CCWWGG 2 cut(s) 675, 750
Bst2BI CACGAG 1 cut(s) 209
Bst2UI CCWGG 2 cut(s) 238, 252
Bst4CI ACNGT 4 cut(s) 326, 384, 719, 995
Bst6I CTCTTC 1 cut(s) 841
BstBAI YACGTR 1 cut(s) 158
BstC8I GCNNGC 3 cut(s) 478, 831, 1066
BstDEI CTNAG 3 cut(s) 32, 302, 441
BstF5I GGATG 4 cut(s) 608, 739, 873, 1117
BstKTI GATC 3 cut(s) 365, 592, 615
BstMAI GTCTC 5 cut(s) 20, 390, 645, 676, 857
BstMBI GATC 3 cut(s) 362, 589, 612
BstMWI GCNNNNNNNGC 6 cut(s) 15, 64, 124, 172, 453, 1070
BstNI CCWGG 2 cut(s) 238, 252
BstNSI RCATGY 3 cut(s) 175, 512, 684
BstSCI CCNGG 4 cut(s) 191, 236, 250, 1196
BstSFI CTRYAG 3 cut(s) 380, 481, 551
BstSLI GKGCMC 1 cut(s) 466
BstV1I GCAGC 7 cut(s) 27, 434, 651, 782, 893, 941, 961
BstX2I RGATCY 1 cut(s) 362
BstYI RGATCY 1 cut(s) 362
Bsu36I CCTNAGG 1 cut(s) 302
BsuRI GGCC 6 cut(s) 166, 188, 456, 755, 833, 1157
BtsCI GGATG 4 cut(s) 608, 739, 873, 1117
BtsI GCAGTG 1 cut(s) 464
BtsIMutI CAGTG 5 cut(s) 29, 464, 673, 825, 1063
Cac8I GCNNGC 3 cut(s) 478, 831, 1066
CaiI CAGNNNCTG 1 cut(s) 974
Cfr13I GGNCC 2 cut(s) 164, 240
Csp6I GTAC 1 cut(s) 561
CviAII CATG 6 cut(s) 10, 172, 509, 628, 681, 1122
CviQI GTAC 1 cut(s) 561
DdeI CTNAG 3 cut(s) 32, 302, 441
DpnI GATC 3 cut(s) 364, 591, 614
DpnII GATC 3 cut(s) 362, 589, 612
DraI TTTAAA 1 cut(s) 882
DrdI GACNNNNNNGTC 1 cut(s) 275
DseDI GACNNNNNNGTC 1 cut(s) 275
EaeI YGGCCR 2 cut(s) 186, 753
Eam1104I CTCTTC 1 cut(s) 841
EarI CTCTTC 1 cut(s) 841
Ecl136II GAGCTC 1 cut(s) 214
Eco130I CCWWGG 2 cut(s) 675, 750
Eco24I GRGCYC 1 cut(s) 216
Eco47I GGWCC 1 cut(s) 240
Eco53kI GAGCTC 1 cut(s) 214
Eco72I CACGTG 1 cut(s) 158
Eco81I CCTNAGG 1 cut(s) 302
EcoICRI GAGCTC 1 cut(s) 214
EcoO109I RGGNCCY 2 cut(s) 164, 240
EcoRII CCWGG 2 cut(s) 236, 250
EcoT14I CCWWGG 2 cut(s) 675, 750
EcoT22I ATGCAT 1 cut(s) 817
EcoT38I GRGCYC 1 cut(s) 216
ErhI CCWWGG 2 cut(s) 675, 750
FaeI CATG 6 cut(s) 13, 175, 512, 631, 684, 1125
FaqI GGGAC 3 cut(s) 95, 214, 283
FatI CATG 6 cut(s) 9, 171, 508, 627, 680, 1121
FauI CCCGC 2 cut(s) 154, 1135
Fnu4HI GCNGC 8 cut(s) 16, 189, 448, 640, 796, 907, 955, 975
FokI GGATG 4 cut(s) 615, 746, 880, 1124
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 8 cut(s) 16, 189, 448, 640, 796, 907, 955, 975
FspBI CTAG 1 cut(s) 389
GluI GCNGC 8 cut(s) 16, 189, 448, 640, 796, 907, 955, 975
GsaI CCCAGC 1 cut(s) 62
GsuI CTGGAG 1 cut(s) 220
HaeIII GGCC 6 cut(s) 166, 188, 456, 755, 833, 1157
HapII CCGG 3 cut(s) 193, 1158, 1197
Hin1II CATG 6 cut(s) 13, 175, 512, 631, 684, 1125
HincII GTYRAC 2 cut(s) 414, 1189
HindII GTYRAC 2 cut(s) 414, 1189
HinfI GANTC 5 cut(s) 263, 348, 575, 737, 893
HpaI GTTAAC 1 cut(s) 414
HpaII CCGG 3 cut(s) 193, 1158, 1197
HphI GGTGA 3 cut(s) 250, 1010, 1186
Hpy166II GTNNAC 2 cut(s) 414, 1189
Hpy188I TCNGA 6 cut(s) 268, 574, 612, 650, 811, 970
Hpy188III TCNNGA 8 cut(s) 209, 389, 579, 697, 773, 856, 932, 941
Hpy8I GTNNAC 2 cut(s) 414, 1189
HpyAV CCTTC 4 cut(s) 215, 253, 467, 1132
HpyCH4III ACNGT 4 cut(s) 326, 384, 719, 995
HpyCH4IV ACGT 1 cut(s) 157
HpyF10VI GCNNNNNNNGC 6 cut(s) 15, 64, 124, 172, 453, 1070
HpyF3I CTNAG 3 cut(s) 32, 302, 441
HpySE526I ACGT 1 cut(s) 157
Hsp92II CATG 6 cut(s) 13, 175, 512, 631, 684, 1125
KspAI GTTAAC 1 cut(s) 414
Kzo9I GATC 3 cut(s) 362, 589, 612
LmnI GCTCC 3 cut(s) 180, 312, 1210
Lsp1109I GCAGC 7 cut(s) 27, 434, 651, 782, 893, 941, 961
LweI GCATC 6 cut(s) 27, 331, 478, 802, 824, 961
MaeI CTAG 1 cut(s) 389
MaeII ACGT 1 cut(s) 157
MaeIII GTNAC 6 cut(s) 256, 334, 375, 527, 719, 800
MalI GATC 3 cut(s) 364, 591, 614
MbiI CCGCTC 1 cut(s) 53
MboI GATC 3 cut(s) 362, 589, 612
MboII GAAGA 2 cut(s) 681, 828
MfeI CAATTG 1 cut(s) 357
MflI RGATCY 1 cut(s) 362
MhlI GDGCHC 3 cut(s) 134, 216, 466
MlsI TGGCCA 1 cut(s) 755
MluCI AATT 6 cut(s) 182, 357, 523, 758, 824, 1131
MluNI TGGCCA 1 cut(s) 755
MlyI GAGTC 3 cut(s) 257, 746, 887
Mox20I TGGCCA 1 cut(s) 755
Mph1103I ATGCAT 1 cut(s) 817
MscI TGGCCA 1 cut(s) 755
MseI TTAA 7 cut(s) 413, 782, 881, 989, 1022, 1031, 1205
MslI CAYNNNNRTG 1 cut(s) 1073
Msp20I TGGCCA 1 cut(s) 755
MspA1I CMGCKG 1 cut(s) 434
MspI CCGG 3 cut(s) 193, 1158, 1197
MspR9I CCNGG 4 cut(s) 193, 238, 252, 1198
MunI CAATTG 1 cut(s) 357
Mva1269I GAATGC 1 cut(s) 909
MvaI CCWGG 2 cut(s) 238, 252
MwoI GCNNNNNNNGC 6 cut(s) 15, 64, 124, 172, 453, 1070
NciI CCSGG 2 cut(s) 193, 1198
NdeII GATC 3 cut(s) 362, 589, 612
NlaIII CATG 6 cut(s) 13, 175, 512, 631, 684, 1125
NlaIV GGNNCC 3 cut(s) 165, 308, 1212
NmuCI GTSAC 4 cut(s) 256, 375, 527, 800
NsiI ATGCAT 1 cut(s) 817
NspI RCATGY 3 cut(s) 175, 512, 684
PctI GAATGC 1 cut(s) 909
PfeI GAWTC 2 cut(s) 348, 575
PkrI GCNGC 8 cut(s) 17, 190, 449, 641, 797, 908, 956, 976
PleI GAGTC 3 cut(s) 257, 745, 887
PmaCI CACGTG 1 cut(s) 158
PmlI CACGTG 1 cut(s) 158
PpsI GAGTC 3 cut(s) 257, 745, 887
Ppu21I YACGTR 1 cut(s) 158
PpuMI RGGWCCY 1 cut(s) 240
PsiI TTATAA 1 cut(s) 491
Psp124BI GAGCTC 1 cut(s) 216
Psp5II RGGWCCY 1 cut(s) 240
Psp6I CCWGG 2 cut(s) 236, 250
PspCI CACGTG 1 cut(s) 158
PspFI CCCAGC 1 cut(s) 58
PspGI CCWGG 2 cut(s) 236, 250
PspN4I GGNNCC 3 cut(s) 165, 308, 1212
PspPI GGNCC 2 cut(s) 164, 240
PspPPI RGGWCCY 1 cut(s) 240
PstI CTGCAG 1 cut(s) 555
PstNI CAGNNNCTG 1 cut(s) 974
PsuI RGATCY 1 cut(s) 362
PvuII CAGCTG 1 cut(s) 434
RsaI GTAC 1 cut(s) 562
RsaNI GTAC 1 cut(s) 561
RseI CAYNNNNRTG 1 cut(s) 1073
SacI GAGCTC 1 cut(s) 216
SaqAI TTAA 7 cut(s) 413, 782, 881, 989, 1022, 1031, 1205
SatI GCNGC 8 cut(s) 16, 189, 448, 640, 796, 907, 955, 975
Sau3AI GATC 3 cut(s) 362, 589, 612
Sau96I GGNCC 2 cut(s) 164, 240
SchI GAGTC 3 cut(s) 257, 746, 887
ScrFI CCNGG 4 cut(s) 193, 238, 252, 1198
SduI GDGCHC 3 cut(s) 134, 216, 466
SfaNI GCATC 6 cut(s) 27, 331, 478, 802, 824, 961
SfcI CTRYAG 3 cut(s) 380, 481, 551
SinI GGWCC 1 cut(s) 240
SmiMI CAYNNNNRTG 1 cut(s) 1073
SmlI CTYRAG 4 cut(s) 695, 704, 854, 932
SmoI CTYRAG 4 cut(s) 695, 704, 854, 932
Sse9I AATT 6 cut(s) 182, 357, 523, 758, 824, 1131
SsiI CCGC 7 cut(s) 53, 153, 161, 178, 189, 865, 1142
SspMI CTAG 1 cut(s) 389
SstI GAGCTC 1 cut(s) 216
StyD4I CCNGG 4 cut(s) 191, 236, 250, 1196
StyI CCWWGG 2 cut(s) 675, 750
TaaI ACNGT 4 cut(s) 326, 384, 719, 995
TaiI ACGT 1 cut(s) 160
TaqI TCGA 3 cut(s) 216, 615, 1005
TasI AATT 6 cut(s) 182, 357, 523, 758, 824, 1131
TatI WGTACW 1 cut(s) 560
TauI GCSGC 1 cut(s) 191
TfiI GAWTC 2 cut(s) 348, 575
Tru1I TTAA 7 cut(s) 413, 782, 881, 989, 1022, 1031, 1205
Tru9I TTAA 7 cut(s) 413, 782, 881, 989, 1022, 1031, 1205
TscAI CASTG 5 cut(s) 36, 471, 673, 825, 1063
TseFI GTSAC 4 cut(s) 256, 375, 527, 800
TseI GCWGC 7 cut(s) 15, 447, 639, 795, 906, 954, 974
Tsp45I GTSAC 4 cut(s) 256, 375, 527, 800
TspDTI ATGAA 3 cut(s) 507, 644, 675
TspGWI ACGGA 1 cut(s) 265
TspRI CASTG 5 cut(s) 36, 471, 673, 825, 1063
VpaK11BI GGWCC 1 cut(s) 240
XapI RAATTY 1 cut(s) 758
XbaI TCTAGA 1 cut(s) 388
XceI RCATGY 3 cut(s) 175, 512, 684
XspI CTAG 1 cut(s) 389
Zsp2I ATGCAT 1 cut(s) 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.