pycom13g00250

Leucine Rich repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0015952
Physical Location & Seq
Reverse (-)
165231 .. 166816
1586 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g00250.1

Sequence Viewer

Length: 1266 bp
ATGGCTTCTTCATCATCAACCGATTCTCCGTCAATAACAATAATGGTATGTATCAACGAGGCGTTGACGGACGACGAGCTCCGGTCGGTGCTAGCGAAGCTGGAGAGCCAGAGAGACAAGGAGGTGTTCGGGTTGGTCTGCAAGCGGTGGCTTCACTTGCAGAGCACGGAGAGGAAGAGGCTCTCCGCACGTGCGGGACCTCACATGCTTCGCAAAATGGCTGCCAGGTTCTACAGGGTCCTGGAGCTCGACCTCTCTCAGTCCATTTCCAGGTCCTTCTATCCCGGTGTCACCGACTCTGATCTGCGTGTTATTGCTGATGGGTTTAAGTGCTTGAGACTCCTCAATCTGCACAACTGCAAAGGAATTTCAGATGCCGGGGTCGTTTTCATTGGCTCTGGTCTTCCATCACTACAGTCCCTAGATGTGTCATATTGTAGAAAGTTAACGGACAAAGGATTATCAGCTGTTGCCCAGGGCTGCGCAGACCTTCGGGGCCTTTATCTTGCTGGCTGCAGATTTGTTACTGATGGGTTATTGCGAGCCCTTTCAAAGAATTGTTACCATTTAGAAGAGTTGGGGCTCCAAGGTTGCACCAACATAACTGACGCTGGACTTACTGATCTTGTAAATGGATGCCAACAGATCAAAGTTTTAGATATCAACAAATGCAGCAATATTGGAGATAGTGGAGTTTCCAGTGTCTCCATGGCATGCTCATCTTCTCTGAAGACGCTGAAGTTGTTGGATTGCTACAAAATCAGTGATGAGTCTATATTGTCGTTGGCCAGATTCTGCAAAAATCTTGAAACTCTAGTCATTGGTGGCTGCCGGGACATTTCAGATGCATCTATAAAATTGCTGGCTGTTTCTTGTAAGAGTAATCTCAAGAACCTACGGATGGATTGGTGTTTAAATATCTCAGACTCTTCGTTGTGCTGTATCCTTGCTCAGTGCAGAAACCTGGAGGTTCTCAACATTGGGTGCTGTGAGGAGGTGACAGATGTCGCCTTTCAGGGTTTAAATGGTGGAGGTGACATTGAGTTGAGTTTGAAGTTTTTAAAGGTGAGTAATTGCCCAAAAGTAACGGTGGCAGGTATAGCCATGGTTTTGGACAAATGTGAGACTTTGGAATATCTGGATGTAAGGTCGTGCCCACACATTACAAAGGCAGGTTGTGAAGAAGGGGGATTGCGGTTTCCTGAACGTTGTAAAGTGAACTTCACAGGGAGTTTAGCTGAGCCTGAGGTGTTACTTTGTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_6

Protein Analysis

422

Amino Acids

45.76

Weight (kDa)

6.54

Isoelectric Point (pI)

49.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 110 - 177 5.9e-08 Leucine Rich Repeat Domain of unknown function (DUF7885)
LRR_At5g56370 PF24758 137 - 241 3.5e-08 FBD-associated F-box protein At5g56370, LRR repeats
DUF7885 PF25372 183 - 394 1.7e-17 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015952)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23340
fragaria_vesca FvH4_4g37160
prunus_persica Prupe.1G349400_v2.0.a1
pyrus_communis pycom13g00250
rosa_chinensis RchiOBHm_Chr4g0446961
rosa_laevigata RLG00000005617
rosa_multiflora Rmu_sc0001753.1_g000005 Rmu_sc0011539.1_g000001
rosa_roxburghii Rroxscaffold_5G00387290
rosa_rugosa Rorug04G0376500
rosa_samantha Rh4AG438000 Rh4BG446900 Rh4CG464200 Rh4DG445700
rosa_wichuraiana Rw4G037460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 484
Acc36I ACCTGC 2 cut(s) 1085, 1163
AciI CCGC 4 cut(s) 145, 186, 194, 1195
AclI AACGTT 1 cut(s) 1207
AcoI YGGCCR 1 cut(s) 786
AcsI RAATTY 1 cut(s) 366
AcuI CTGAAG 2 cut(s) 749, 758
AcvI CACGTG 1 cut(s) 191
AfiI CCNNNNNNNGG 2 cut(s) 270, 901
AgsI TTSAA 3 cut(s) 552, 809, 1054
AjnI CCWGG 5 cut(s) 224, 240, 269, 474, 963
AluBI AGCT 5 cut(s) 79, 100, 247, 467, 1238
AluI AGCT 5 cut(s) 79, 100, 247, 467, 1238
Alw21I GWGCWC 3 cut(s) 81, 167, 249
Alw26I GTCTC 4 cut(s) 108, 331, 709, 1118
AlwNI CAGNNNCTG 1 cut(s) 795
AoxI GGCC 2 cut(s) 496, 786
ApeKI GCWGC 5 cut(s) 221, 480, 513, 672, 828
ApoI RAATTY 1 cut(s) 366
AspLEI GCGC 1 cut(s) 485
AspS9I GGNCC 4 cut(s) 197, 238, 273, 496
AsuC2I CCSGG 3 cut(s) 285, 379, 833
AsuHPI GGTGA 4 cut(s) 283, 1009, 1046, 1078
AsuNHI GCTAGC 1 cut(s) 91
AvaII GGWCC 3 cut(s) 197, 238, 273
AxyI CCTNAGG 1 cut(s) 1245
BaeGI GKGCMC 1 cut(s) 1157
BalI TGGCCA 1 cut(s) 788
BanII GRGCYC 4 cut(s) 81, 249, 547, 585
BbrPI CACGTG 1 cut(s) 191
BbsI GAAGAC 2 cut(s) 395, 737
Bbv12I GWGCWC 3 cut(s) 81, 167, 249
BbvI GCAGC 5 cut(s) 208, 467, 500, 684, 815
BccI CCATC 4 cut(s) 314, 415, 524, 895
BciT130I CCWGG 5 cut(s) 226, 242, 271, 476, 965
BciVI GTATCC 1 cut(s) 953
BcnI CCSGG 3 cut(s) 285, 379, 833
BcoDI GTCTC 4 cut(s) 108, 331, 709, 1118
BfaI CTAG 3 cut(s) 92, 422, 815
BfmI CTRYAG 3 cut(s) 232, 413, 514
BfuAI ACCTGC 2 cut(s) 1085, 1163
BfuI GTATCC 1 cut(s) 953
BisI GCNGC 5 cut(s) 222, 481, 514, 673, 829
BlpI GCTNAGC 1 cut(s) 1239
BlsI GCNGC 5 cut(s) 223, 482, 515, 674, 830
Bme1390I CCNGG 8 cut(s) 226, 242, 271, 285, 379, 476, 833, 965
Bme18I GGWCC 3 cut(s) 197, 238, 273
BmgT120I GGNCC 4 cut(s) 197, 238, 273, 496
BmiI GGNNCC 4 cut(s) 198, 239, 497, 584
BmrFI CCNGG 8 cut(s) 226, 242, 271, 285, 379, 476, 833, 965
BmsI GCATC 4 cut(s) 364, 626, 835, 857
BmtI GCTAGC 1 cut(s) 95
BoxI GACNNNNGTC 1 cut(s) 1004
BpiI GAAGAC 2 cut(s) 395, 737
BpmI CTGGAG 3 cut(s) 122, 263, 986
Bpu1102I GCTNAGC 1 cut(s) 1239
BpuEI CTTGAG 2 cut(s) 355, 872
BpuMI CCSGG 3 cut(s) 285, 379, 833
BsaAI YACGTR 1 cut(s) 191
BsaJI CCNNGG 6 cut(s) 378, 474, 475, 586, 708, 1104
BsaWI WCCGGW 1 cut(s) 81
BsaXI ACNNNNNCTCC 2 cut(s) 10, 40
Bsc4I CCNNNNNNNGG 2 cut(s) 270, 901
Bse1I ACTGG 1 cut(s) 699
Bse21I CCTNAGG 1 cut(s) 1245
BseBI CCWGG 5 cut(s) 226, 242, 271, 476, 965
BseDI CCNNGG 6 cut(s) 378, 474, 475, 586, 708, 1104
BseGI GGATG 3 cut(s) 641, 906, 1147
BseLI CCNNNNNNNGG 2 cut(s) 270, 901
BseMII CTCAG 5 cut(s) 272, 936, 965, 1230, 1236
BseNI ACTGG 1 cut(s) 699
BseRI GAGGAG 2 cut(s) 332, 1007
BseSI GKGCMC 1 cut(s) 1157
BseXI GCAGC 5 cut(s) 208, 467, 500, 684, 815
BsgI GTGCAG 2 cut(s) 335, 976
Bsh1285I CGRYCG 1 cut(s) 86
BshFI GGCC 2 cut(s) 498, 788
BsiEI CGRYCG 1 cut(s) 86
BsiHKAI GWGCWC 3 cut(s) 81, 167, 249
BsiSI CCGG 4 cut(s) 82, 285, 378, 832
BslFI GGGAC 3 cut(s) 210, 403, 848
BslI CCNNNNNNNGG 2 cut(s) 270, 901
BsmAI GTCTC 4 cut(s) 108, 331, 709, 1118
BsmFI GGGAC 3 cut(s) 210, 403, 848
BsnI GGCC 2 cut(s) 498, 788
Bsp1286I GDGCHC 6 cut(s) 81, 167, 249, 547, 585, 1157
Bsp143I GATC 3 cut(s) 301, 622, 645
Bsp1720I GCTNAGC 1 cut(s) 1239
Bsp19I CCATGG 2 cut(s) 708, 1104
BspACI CCGC 4 cut(s) 145, 186, 194, 1195
BspANI GGCC 2 cut(s) 498, 788
BspCNI CTCAG 5 cut(s) 271, 935, 964, 1231, 1237
BspLI GGNNCC 4 cut(s) 198, 239, 497, 584
BspMAI CTGCAG 1 cut(s) 518
BspMI ACCTGC 2 cut(s) 1085, 1163
BspOI GCTAGC 1 cut(s) 95
BsrI ACTGG 1 cut(s) 699
BssECI CCNNGG 6 cut(s) 378, 474, 475, 586, 708, 1104
BssMI GATC 3 cut(s) 301, 622, 645
BssT1I CCWWGG 3 cut(s) 586, 708, 1104
Bst2UI CCWGG 5 cut(s) 226, 242, 271, 476, 965
Bst4CI ACNGT 2 cut(s) 417, 1090
Bst6I CTCTTC 3 cut(s) 170, 567, 934
BstBAI YACGTR 1 cut(s) 191
BstC8I GCNNGC 6 cut(s) 93, 143, 511, 543, 715, 864
BstDEI CTNAG 5 cut(s) 258, 922, 951, 1239, 1245
BstDSI CCRYGG 2 cut(s) 708, 1104
BstF5I GGATG 3 cut(s) 641, 906, 1147
BstHHI GCGC 1 cut(s) 485
BstKTI GATC 3 cut(s) 304, 625, 648
BstMAI GTCTC 4 cut(s) 108, 331, 709, 1118
BstMBI GATC 3 cut(s) 301, 622, 645
BstMCI CGRYCG 1 cut(s) 86
BstMWI GCNNNNNNNGC 3 cut(s) 97, 157, 1100
BstNI CCWGG 5 cut(s) 226, 242, 271, 476, 965
BstNSI RCATGY 2 cut(s) 208, 717
BstPAI GACNNNNGTC 1 cut(s) 1004
BstSCI CCNGG 8 cut(s) 224, 240, 269, 283, 377, 474, 831, 963
BstSFI CTRYAG 3 cut(s) 232, 413, 514
BstSLI GKGCMC 1 cut(s) 1157
BstV1I GCAGC 5 cut(s) 208, 467, 500, 684, 815
BstV2I GAAGAC 2 cut(s) 395, 737
BstXI CCANNNNNNTGG 1 cut(s) 1111
Bsu36I CCTNAGG 1 cut(s) 1245
BsuI GTATCC 1 cut(s) 953
BsuRI GGCC 2 cut(s) 498, 788
BtgI CCRYGG 2 cut(s) 708, 1104
BtsCI GGATG 3 cut(s) 641, 906, 1147
BtsIMutI CAGTG 3 cut(s) 706, 769, 959
BveI ACCTGC 2 cut(s) 1085, 1163
Cac8I GCNNGC 6 cut(s) 93, 143, 511, 543, 715, 864
CaiI CAGNNNCTG 1 cut(s) 795
CfoI GCGC 1 cut(s) 485
Cfr13I GGNCC 4 cut(s) 197, 238, 273, 496
CseI GACGC 2 cut(s) 617, 742
CviAII CATG 4 cut(s) 205, 709, 714, 1105
DdeI CTNAG 5 cut(s) 258, 922, 951, 1239, 1245
DpnI GATC 3 cut(s) 303, 624, 647
DpnII GATC 3 cut(s) 301, 622, 645
DraI TTTAAA 3 cut(s) 915, 1023, 1062
EaeI YGGCCR 1 cut(s) 786
Eam1104I CTCTTC 3 cut(s) 170, 567, 934
EarI CTCTTC 3 cut(s) 170, 567, 934
Ecl136II GAGCTC 2 cut(s) 79, 247
Eco130I CCWWGG 3 cut(s) 586, 708, 1104
Eco24I GRGCYC 4 cut(s) 81, 249, 547, 585
Eco32I GATATC 1 cut(s) 661
Eco47I GGWCC 3 cut(s) 197, 238, 273
Eco53kI GAGCTC 2 cut(s) 79, 247
Eco57I CTGAAG 2 cut(s) 749, 758
Eco72I CACGTG 1 cut(s) 191
Eco81I CCTNAGG 1 cut(s) 1245
EcoICRI GAGCTC 2 cut(s) 79, 247
EcoO109I RGGNCCY 4 cut(s) 197, 238, 273, 496
EcoRII CCWGG 5 cut(s) 224, 240, 269, 474, 963
EcoRV GATATC 1 cut(s) 661
EcoT14I CCWWGG 3 cut(s) 586, 708, 1104
EcoT22I ATGCAT 1 cut(s) 850
EcoT38I GRGCYC 4 cut(s) 81, 249, 547, 585
ErhI CCWWGG 3 cut(s) 586, 708, 1104
FaeI CATG 4 cut(s) 208, 712, 717, 1108
FaqI GGGAC 3 cut(s) 210, 403, 848
FatI CATG 4 cut(s) 204, 708, 713, 1104
FauI CCCGC 1 cut(s) 187
Fnu4HI GCNGC 5 cut(s) 222, 481, 514, 673, 829
FokI GGATG 3 cut(s) 648, 913, 1154
FriOI GRGCYC 4 cut(s) 81, 249, 547, 585
Fsp4HI GCNGC 5 cut(s) 222, 481, 514, 673, 829
FspBI CTAG 3 cut(s) 92, 422, 815
FspI TGCGCA 1 cut(s) 484
GlaI GCGC 1 cut(s) 484
GluI GCNGC 5 cut(s) 222, 481, 514, 673, 829
GsuI CTGGAG 3 cut(s) 122, 263, 986
HaeIII GGCC 2 cut(s) 498, 788
HapII CCGG 4 cut(s) 82, 285, 378, 832
HgaI GACGC 2 cut(s) 617, 742
HhaI GCGC 1 cut(s) 485
Hin1II CATG 4 cut(s) 208, 712, 717, 1108
Hin6I GCGC 1 cut(s) 483
HinP1I GCGC 1 cut(s) 483
HincII GTYRAC 2 cut(s) 66, 447
HindII GTYRAC 2 cut(s) 66, 447
HinfI GANTC 6 cut(s) 23, 296, 339, 770, 792, 926
HpaI GTTAAC 1 cut(s) 447
HpaII CCGG 4 cut(s) 82, 285, 378, 832
HphI GGTGA 4 cut(s) 283, 1009, 1046, 1078
Hpy166II GTNNAC 3 cut(s) 66, 447, 1219
Hpy188I TCNGA 5 cut(s) 301, 373, 729, 844, 925
Hpy188III TCNNGA 4 cut(s) 806, 889, 1139, 1202
Hpy8I GTNNAC 3 cut(s) 66, 447, 1219
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 3 cut(s) 286, 500, 1178
HpyCH4III ACNGT 2 cut(s) 417, 1090
HpyCH4IV ACGT 2 cut(s) 190, 1207
HpyF10VI GCNNNNNNNGC 3 cut(s) 97, 157, 1100
HpyF3I CTNAG 5 cut(s) 258, 922, 951, 1239, 1245
HpySE526I ACGT 2 cut(s) 190, 1207
Hsp92II CATG 4 cut(s) 208, 712, 717, 1108
HspAI GCGC 1 cut(s) 483
KspAI GTTAAC 1 cut(s) 447
Kzo9I GATC 3 cut(s) 301, 622, 645
LmnI GCTCC 3 cut(s) 84, 244, 588
Lsp1109I GCAGC 5 cut(s) 208, 467, 500, 684, 815
LweI GCATC 4 cut(s) 364, 626, 835, 857
MaeI CTAG 3 cut(s) 92, 422, 815
MaeII ACGT 2 cut(s) 190, 1207
MaeIII GTNAC 7 cut(s) 289, 523, 560, 997, 1034, 1084, 1251
MalI GATC 3 cut(s) 303, 624, 647
MboI GATC 3 cut(s) 301, 622, 645
MboII GAAGA 7 cut(s) 187, 395, 584, 714, 742, 921, 1193
MhlI GDGCHC 6 cut(s) 81, 167, 249, 547, 585, 1157
MlsI TGGCCA 1 cut(s) 788
MluCI AATT 4 cut(s) 366, 556, 857, 1072
MluNI TGGCCA 1 cut(s) 788
MlyI GAGTC 4 cut(s) 290, 333, 779, 920
MmeI TCCRAC 1 cut(s) 726
Mox20I TGGCCA 1 cut(s) 788
Mph1103I ATGCAT 1 cut(s) 850
MscI TGGCCA 1 cut(s) 788
MseI TTAA 5 cut(s) 327, 446, 914, 1022, 1061
Msp20I TGGCCA 1 cut(s) 788
MspA1I CMGCKG 1 cut(s) 467
MspI CCGG 4 cut(s) 82, 285, 378, 832
MspR9I CCNGG 8 cut(s) 226, 242, 271, 285, 379, 476, 833, 965
MvaI CCWGG 5 cut(s) 226, 242, 271, 476, 965
MwoI GCNNNNNNNGC 3 cut(s) 97, 157, 1100
NciI CCSGG 3 cut(s) 285, 379, 833
NcoI CCATGG 2 cut(s) 708, 1104
NdeII GATC 3 cut(s) 301, 622, 645
NheI GCTAGC 1 cut(s) 91
NlaIII CATG 4 cut(s) 208, 712, 717, 1108
NlaIV GGNNCC 4 cut(s) 198, 239, 497, 584
NmuCI GTSAC 3 cut(s) 289, 997, 1034
NsbI TGCGCA 1 cut(s) 484
NsiI ATGCAT 1 cut(s) 850
NspI RCATGY 2 cut(s) 208, 717
PaeI GCATGC 1 cut(s) 717
PasI CCCWGGG 1 cut(s) 475
PcsI WCGNNNNNNNCGW 1 cut(s) 92
PfeI GAWTC 2 cut(s) 23, 792
PfoI TCCNGGA 1 cut(s) 240
PkrI GCNGC 5 cut(s) 223, 482, 515, 674, 830
PleI GAGTC 4 cut(s) 290, 333, 778, 920
PmaCI CACGTG 1 cut(s) 191
PmlI CACGTG 1 cut(s) 191
PpsI GAGTC 4 cut(s) 290, 333, 778, 920
Ppu21I YACGTR 1 cut(s) 191
PpuMI RGGWCCY 3 cut(s) 197, 238, 273
PshAI GACNNNNGTC 1 cut(s) 1004
Psp124BI GAGCTC 2 cut(s) 81, 249
Psp1406I AACGTT 1 cut(s) 1207
Psp5II RGGWCCY 3 cut(s) 197, 238, 273
Psp6I CCWGG 5 cut(s) 224, 240, 269, 474, 963
PspCI CACGTG 1 cut(s) 191
PspGI CCWGG 5 cut(s) 224, 240, 269, 474, 963
PspN4I GGNNCC 4 cut(s) 198, 239, 497, 584
PspPI GGNCC 4 cut(s) 197, 238, 273, 496
PspPPI RGGWCCY 3 cut(s) 197, 238, 273
PstI CTGCAG 1 cut(s) 518
PstNI CAGNNNCTG 1 cut(s) 795
PvuII CAGCTG 1 cut(s) 467
SacI GAGCTC 2 cut(s) 81, 249
SaqAI TTAA 5 cut(s) 327, 446, 914, 1022, 1061
SatI GCNGC 5 cut(s) 222, 481, 514, 673, 829
Sau3AI GATC 3 cut(s) 301, 622, 645
Sau96I GGNCC 4 cut(s) 197, 238, 273, 496
SchI GAGTC 4 cut(s) 290, 333, 779, 920
ScrFI CCNGG 8 cut(s) 226, 242, 271, 285, 379, 476, 833, 965
SduI GDGCHC 6 cut(s) 81, 167, 249, 547, 585, 1157
SfaNI GCATC 4 cut(s) 364, 626, 835, 857
SfcI CTRYAG 3 cut(s) 232, 413, 514
SinI GGWCC 3 cut(s) 197, 238, 273
SmlI CTYRAG 2 cut(s) 334, 887
SmoI CTYRAG 2 cut(s) 334, 887
SphI GCATGC 1 cut(s) 717
Sse9I AATT 4 cut(s) 366, 556, 857, 1072
SsiI CCGC 4 cut(s) 145, 186, 194, 1195
SspI AATATT 1 cut(s) 679
SspMI CTAG 3 cut(s) 92, 422, 815
SstI GAGCTC 2 cut(s) 81, 249
StyD4I CCNGG 8 cut(s) 224, 240, 269, 283, 377, 474, 831, 963
StyI CCWWGG 3 cut(s) 586, 708, 1104
TaaI ACNGT 2 cut(s) 417, 1090
TaiI ACGT 2 cut(s) 193, 1210
TaqI TCGA 1 cut(s) 249
TasI AATT 4 cut(s) 366, 556, 857, 1072
TfiI GAWTC 2 cut(s) 23, 792
Tru1I TTAA 5 cut(s) 327, 446, 914, 1022, 1061
Tru9I TTAA 5 cut(s) 327, 446, 914, 1022, 1061
TscAI CASTG 3 cut(s) 706, 769, 959
TseFI GTSAC 3 cut(s) 289, 997, 1034
TseI GCWGC 5 cut(s) 221, 480, 513, 672, 828
Tsp45I GTSAC 3 cut(s) 289, 997, 1034
TspDTI ATGAA 1 cut(s) 379
TspGWI ACGGA 5 cut(s) 18, 83, 182, 464, 913
TspRI CASTG 3 cut(s) 706, 769, 959
VpaK11BI GGWCC 3 cut(s) 197, 238, 273
XapI RAATTY 1 cut(s) 366
XceI RCATGY 2 cut(s) 208, 717
XcmI CCANNNNNNNNNTGG 1 cut(s) 706
XspI CTAG 3 cut(s) 92, 422, 815
Zsp2I ATGCAT 1 cut(s) 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.