Rh4BG446900

Leucine Rich repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0015952
Physical Location & Seq
Forward (+)
58557425 .. 58558851
1427 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG446900.1

Sequence Viewer

Length: 1224 bp
ATGGGCGGAAGCATCAACGAGGCACTGAGAGATGACGAGCTTCGATCGGTGCTGGCGAAGCTGGAGAGTCAGCGGGACAAGGAGGTGTTCGGGTTGGTGTGCAAGAGGTGGCTTCACTTGCAGAGCACTGAGAGGAGGAAGCTGTCCGCACGTGCGGGGCCTCACATGCTCCGCAAAATGGCCGCCAGGTTCTCCCGCCTTCATGAGCTCGACCTCTCCCAGTCCCTTTCCAGGTCATTCTATCCAGATGTCACCGACTCTGATCTCTCCGTCATTGCCAATGGCTTCAACTGCTTGAGGCTCCTCACTCTCCAAAACTGTAAAGGAGTTACGGATGCTGGAATCATTGCAATTGGATCTGGTCTTTCATCACTACAGTCTCTAGATGTGTCGTATTGCAGAAAGTTAACAGACAAAGGATTATCAGCTGTTGCTCAGGGCTGCTCTGACCTTCGCGCACTGCATCTTGCCGGCTGCAGATTTATAACCGATGCATTATTACATGCTCTTTCAAACAATTGTCACTCTTTACAACACTTGGCTCTCCAAGGCTGTACTAATATCTCCGATTCTGGACTTAGTGATCTTGTAAATGGATGTCAACAGATCGAGTTTTTAGACATCAACAAATGCAGCAACATTGGAGACATTGGCGTTTCCAGTGTCTCCAAGGCATGTTCATCTTCTCTCAAGACACTCAAGCTATTGGACTGTTACAAAGTCGGGGATGAGTCTATAATATCCTTGGCCACATTCTGCAAAAATCTTGAAACTTTAATCATTGGTGGCTGCCGGGACATATCTGACGCATCAATAAATTTGCTGGCCTCTTCTTGTAAGGCTAGTCTCAAGAACTTACGGATGGATTGGTGTTTAAATATCACAGACTCTTCTTTGAGCTGCATTCTGGTTCAGTGCAGAAACCTCGAGGCTCTTGACATTGGGTGCTGCGAGGAGGTGTCAGATGCTGCCTTTCAGGGTTTAAAAGGTGGTGAAGTTGAGCTGACCCTGACCTTAAAGGTCTTAAAGGTTAGTAACTGCCCGAAAATCAGTGTGGCAGGGATAGCTATGGTGTTGGAGAAATGCGAGATGCTAAAATACTTGGATGTAAGGTCATGCCCACAAATTACAAAGGGGGGCTGTGATGAGGCCGGATTGCAGTTTCCTCAATCTTGTAAAGTCAACTTCACCGGGAGTTTAGGGGAGCCTGATGTCTTACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_6

Protein Analysis

407

Amino Acids

43.91

Weight (kDa)

6.4

Isoelectric Point (pI)

48.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 62 - 171 4.8e-12 Leucine Rich Repeat Domain of unknown function (DUF7885)
LRR_At5g56370 PF24758 123 - 235 2.3e-08 FBD-associated F-box protein At5g56370, LRR repeats
DUF7885 PF25372 170 - 381 2.3e-18 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015952)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23340
fragaria_vesca FvH4_4g37160
prunus_persica Prupe.1G349400_v2.0.a1
pyrus_communis pycom13g00250
rosa_chinensis RchiOBHm_Chr4g0446961
rosa_laevigata RLG00000005617
rosa_multiflora Rmu_sc0001753.1_g000005 Rmu_sc0011539.1_g000001
rosa_roxburghii Rroxscaffold_5G00387290
rosa_rugosa Rorug04G0376500
rosa_samantha Rh4AG438000 Rh4BG446900 Rh4CG464200 Rh4DG445700
rosa_wichuraiana Rw4G037460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 485
AbsI CCTCGAGG 1 cut(s) 926
AccII CGCG 1 cut(s) 456
AciI CCGC 7 cut(s) 6, 73, 147, 155, 172, 183, 196
AclWI GGATC 1 cut(s) 364
AcoI YGGCCR 2 cut(s) 180, 747
AcsI RAATTY 1 cut(s) 817
AcvI CACGTG 1 cut(s) 152
AfaI GTAC 1 cut(s) 556
AfiI CCNNNNNNNGG 2 cut(s) 178, 231
AgsI TTSAA 3 cut(s) 289, 513, 770
AjnI CCWGG 2 cut(s) 185, 230
AluBI AGCT 9 cut(s) 40, 61, 142, 208, 428, 703, 900, 1003, 1067
AluI AGCT 9 cut(s) 40, 61, 142, 208, 428, 703, 900, 1003, 1067
Alw21I GWGCWC 2 cut(s) 128, 210
Alw26I GTCTC 4 cut(s) 384, 639, 670, 851
AlwI GGATC 1 cut(s) 364
AlwNI CAGNNNCTG 1 cut(s) 968
Ama87I CYCGRG 1 cut(s) 926
AoxI GGCC 5 cut(s) 158, 180, 747, 825, 1149
ApeKI GCWGC 7 cut(s) 441, 474, 633, 789, 900, 948, 968
ApoI RAATTY 1 cut(s) 817
AspLEI GCGC 1 cut(s) 458
AspS9I GGNCC 1 cut(s) 158
AsuC2I CCSGG 2 cut(s) 794, 1192
AsuHPI GGTGA 3 cut(s) 244, 1004, 1180
AvaI CYCGRG 1 cut(s) 926
BalI TGGCCA 1 cut(s) 749
BanII GRGCYC 1 cut(s) 210
BbrPI CACGTG 1 cut(s) 152
Bbv12I GWGCWC 2 cut(s) 128, 210
BbvI GCAGC 7 cut(s) 428, 461, 645, 776, 887, 935, 955
BccI CCATC 1 cut(s) 856
BcgI CGANNNNNNTGC 2 cut(s) 907, 941
BciT130I CCWGG 2 cut(s) 187, 232
BcnI CCSGG 2 cut(s) 794, 1192
BcoDI GTCTC 4 cut(s) 384, 639, 670, 851
BfaI CTAG 2 cut(s) 383, 843
BfmI CTRYAG 2 cut(s) 374, 475
BisI GCNGC 8 cut(s) 183, 442, 475, 634, 790, 901, 949, 969
BlsI GCNGC 8 cut(s) 184, 443, 476, 635, 791, 902, 950, 970
Bme1390I CCNGG 4 cut(s) 187, 232, 794, 1192
BmeT110I CYCGRG 1 cut(s) 926
BmgT120I GGNCC 1 cut(s) 158
BmiI GGNNCC 3 cut(s) 159, 302, 1206
BmrFI CCNGG 4 cut(s) 187, 232, 794, 1192
BmrI ACTGGG 1 cut(s) 214
BmsI GCATC 7 cut(s) 21, 325, 472, 481, 818, 955, 1080
BmuI ACTGGG 1 cut(s) 214
BpmI CTGGAG 1 cut(s) 83
Bpu10I CCTNAGC 1 cut(s) 435
BpuEI CTTGAG 4 cut(s) 316, 674, 683, 833
BpuMI CCSGG 2 cut(s) 794, 1192
BsaAI YACGTR 1 cut(s) 152
BsaJI CCNNGG 3 cut(s) 547, 669, 744
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 231
Bse118I RCCGGY 1 cut(s) 470
Bse1I ACTGG 2 cut(s) 220, 660
Bse3DI GCAATG 2 cut(s) 273, 345
BseBI CCWGG 2 cut(s) 187, 232
BseDI CCNNGG 3 cut(s) 547, 669, 744
BseGI GGATG 5 cut(s) 340, 602, 733, 867, 1111
BseLI CCNNNNNNNGG 2 cut(s) 178, 231
BseMI GCAATG 2 cut(s) 273, 345
BseMII CTCAG 3 cut(s) 17, 120, 449
BseNI ACTGG 2 cut(s) 220, 660
BseRI GAGGAG 3 cut(s) 148, 293, 968
BseXI GCAGC 7 cut(s) 428, 461, 645, 776, 887, 935, 955
BsgI GTGCAG 1 cut(s) 937
Bsh1236I CGCG 1 cut(s) 456
Bsh1285I CGRYCG 1 cut(s) 47
BshFI GGCC 5 cut(s) 160, 182, 749, 827, 1151
BsiEI CGRYCG 1 cut(s) 47
BsiHKAI GWGCWC 2 cut(s) 128, 210
BsiHKCI CYCGRG 1 cut(s) 926
BsiSI CCGG 4 cut(s) 471, 793, 1152, 1191
BslFI GGGAC 3 cut(s) 89, 208, 809
BslI CCNNNNNNNGG 2 cut(s) 178, 231
BsmAI GTCTC 4 cut(s) 384, 639, 670, 851
BsmFI GGGAC 3 cut(s) 89, 208, 809
BsmI GAATGC 1 cut(s) 903
BsnI GGCC 5 cut(s) 160, 182, 749, 827, 1151
BsoBI CYCGRG 1 cut(s) 926
Bsp1286I GDGCHC 2 cut(s) 128, 210
Bsp143I GATC 5 cut(s) 44, 262, 356, 583, 606
BspACI CCGC 7 cut(s) 6, 73, 147, 155, 172, 183, 196
BspANI GGCC 5 cut(s) 160, 182, 749, 827, 1151
BspCNI CTCAG 3 cut(s) 18, 121, 448
BspFNI CGCG 1 cut(s) 456
BspHI TCATGA 1 cut(s) 202
BspLI GGNNCC 3 cut(s) 159, 302, 1206
BspMAI CTGCAG 1 cut(s) 479
BspPI GGATC 1 cut(s) 364
BsrDI GCAATG 2 cut(s) 273, 345
BsrFI RCCGGY 1 cut(s) 470
BsrI ACTGG 2 cut(s) 220, 660
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 3 cut(s) 547, 669, 744
BssMI GATC 5 cut(s) 44, 262, 356, 583, 606
BssT1I CCWWGG 3 cut(s) 547, 669, 744
Bst2UI CCWGG 2 cut(s) 187, 232
Bst4CI ACNGT 3 cut(s) 320, 378, 713
Bst6I CTCTTC 2 cut(s) 835, 895
BstBAI YACGTR 1 cut(s) 152
BstC8I GCNNGC 3 cut(s) 54, 472, 825
BstDEI CTNAG 4 cut(s) 26, 129, 435, 578
BstF5I GGATG 5 cut(s) 340, 602, 733, 867, 1111
BstFNI CGCG 1 cut(s) 456
BstHHI GCGC 1 cut(s) 458
BstKTI GATC 5 cut(s) 47, 265, 359, 586, 609
BstMAI GTCTC 4 cut(s) 384, 639, 670, 851
BstMBI GATC 5 cut(s) 44, 262, 356, 583, 606
BstMCI CGRYCG 1 cut(s) 47
BstMWI GCNNNNNNNGC 5 cut(s) 58, 118, 166, 291, 1064
BstNI CCWGG 2 cut(s) 187, 232
BstNSI RCATGY 3 cut(s) 169, 506, 678
BstSCI CCNGG 4 cut(s) 185, 230, 792, 1190
BstSFI CTRYAG 2 cut(s) 374, 475
BstUI CGCG 1 cut(s) 456
BstV1I GCAGC 7 cut(s) 428, 461, 645, 776, 887, 935, 955
BstX2I RGATCY 1 cut(s) 356
BstYI RGATCY 1 cut(s) 356
BsuRI GGCC 5 cut(s) 160, 182, 749, 827, 1151
BtsCI GGATG 5 cut(s) 340, 602, 733, 867, 1111
BtsI GCAGTG 1 cut(s) 458
BtsIMutI CAGTG 6 cut(s) 23, 126, 458, 667, 920, 1057
Cac8I GCNNGC 3 cut(s) 54, 472, 825
CaiI CAGNNNCTG 1 cut(s) 968
CciI TCATGA 1 cut(s) 202
CfoI GCGC 1 cut(s) 458
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 1 cut(s) 158
CseI GACGC 1 cut(s) 815
Csp6I GTAC 1 cut(s) 555
CviAII CATG 5 cut(s) 166, 203, 503, 675, 1116
CviQI GTAC 1 cut(s) 555
DdeI CTNAG 4 cut(s) 26, 129, 435, 578
DpnI GATC 5 cut(s) 46, 264, 358, 585, 608
DpnII GATC 5 cut(s) 44, 262, 356, 583, 606
DraI TTTAAA 2 cut(s) 876, 984
EaeI YGGCCR 2 cut(s) 180, 747
Eam1104I CTCTTC 2 cut(s) 835, 895
EarI CTCTTC 2 cut(s) 835, 895
EciI GGCGGA 1 cut(s) 21
Ecl136II GAGCTC 1 cut(s) 208
Eco130I CCWWGG 3 cut(s) 547, 669, 744
Eco24I GRGCYC 1 cut(s) 210
Eco53kI GAGCTC 1 cut(s) 208
Eco72I CACGTG 1 cut(s) 152
Eco88I CYCGRG 1 cut(s) 926
EcoICRI GAGCTC 1 cut(s) 208
EcoO109I RGGNCCY 1 cut(s) 158
EcoRII CCWGG 2 cut(s) 185, 230
EcoT14I CCWWGG 3 cut(s) 547, 669, 744
EcoT22I ATGCAT 1 cut(s) 496
EcoT38I GRGCYC 1 cut(s) 210
ErhI CCWWGG 3 cut(s) 547, 669, 744
FaeI CATG 5 cut(s) 169, 206, 506, 678, 1119
FaiI YATR 9 cut(s) 167, 204, 485, 504, 676, 737, 800, 1070, 1117
FaqI GGGAC 3 cut(s) 89, 208, 809
FatI CATG 5 cut(s) 165, 202, 502, 674, 1115
FauI CCCGC 3 cut(s) 66, 148, 203
Fnu4HI GCNGC 8 cut(s) 183, 442, 475, 634, 790, 901, 949, 969
FokI GGATG 5 cut(s) 347, 609, 740, 874, 1118
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 8 cut(s) 183, 442, 475, 634, 790, 901, 949, 969
FspBI CTAG 2 cut(s) 383, 843
GlaI GCGC 1 cut(s) 457
GluI GCNGC 8 cut(s) 183, 442, 475, 634, 790, 901, 949, 969
GsuI CTGGAG 1 cut(s) 83
HaeIII GGCC 5 cut(s) 160, 182, 749, 827, 1151
HapII CCGG 4 cut(s) 471, 793, 1152, 1191
HgaI GACGC 1 cut(s) 815
HhaI GCGC 1 cut(s) 458
Hin1II CATG 5 cut(s) 169, 206, 506, 678, 1119
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HincII GTYRAC 3 cut(s) 408, 602, 1183
HindII GTYRAC 3 cut(s) 408, 602, 1183
HinfI GANTC 6 cut(s) 67, 257, 342, 569, 731, 887
HpaI GTTAAC 1 cut(s) 408
HpaII CCGG 4 cut(s) 471, 793, 1152, 1191
HphI GGTGA 3 cut(s) 244, 1004, 1180
Hpy166II GTNNAC 3 cut(s) 408, 602, 1183
Hpy188I TCNGA 5 cut(s) 262, 448, 568, 805, 964
Hpy188III TCNNGA 8 cut(s) 203, 245, 383, 573, 691, 767, 850, 935
Hpy8I GTNNAC 3 cut(s) 408, 602, 1183
HpyAV CCTTC 2 cut(s) 209, 461
HpyCH4III ACNGT 3 cut(s) 320, 378, 713
HpyCH4IV ACGT 1 cut(s) 151
HpyF10VI GCNNNNNNNGC 5 cut(s) 58, 118, 166, 291, 1064
HpyF3I CTNAG 4 cut(s) 26, 129, 435, 578
HpySE526I ACGT 1 cut(s) 151
Hsp92II CATG 5 cut(s) 169, 206, 506, 678, 1119
HspAI GCGC 1 cut(s) 456
KroI GCCGGC 1 cut(s) 470
KroNI GCCGGC 1 cut(s) 472
KspAI GTTAAC 1 cut(s) 408
Kzo9I GATC 5 cut(s) 44, 262, 356, 583, 606
LmnI GCTCC 3 cut(s) 174, 306, 1204
Lsp1109I GCAGC 7 cut(s) 428, 461, 645, 776, 887, 935, 955
LweI GCATC 7 cut(s) 21, 325, 472, 481, 818, 955, 1080
MaeI CTAG 2 cut(s) 383, 843
MaeII ACGT 1 cut(s) 151
MaeIII GTNAC 5 cut(s) 250, 328, 521, 713, 1034
MalI GATC 5 cut(s) 46, 264, 358, 585, 608
MboI GATC 5 cut(s) 44, 262, 356, 583, 606
MboII GAAGA 3 cut(s) 675, 822, 882
MfeI CAATTG 2 cut(s) 351, 517
MflI RGATCY 1 cut(s) 356
MhlI GDGCHC 2 cut(s) 128, 210
MlsI TGGCCA 1 cut(s) 749
MluCI AATT 4 cut(s) 351, 517, 817, 1125
MluNI TGGCCA 1 cut(s) 749
MlyI GAGTC 4 cut(s) 76, 251, 740, 881
MmeI TCCRAC 1 cut(s) 1056
Mox20I TGGCCA 1 cut(s) 749
Mph1103I ATGCAT 1 cut(s) 496
MroNI GCCGGC 1 cut(s) 470
MscI TGGCCA 1 cut(s) 749
MseI TTAA 6 cut(s) 407, 776, 875, 983, 1016, 1025
Msp20I TGGCCA 1 cut(s) 749
MspA1I CMGCKG 2 cut(s) 73, 428
MspI CCGG 4 cut(s) 471, 793, 1152, 1191
MspR9I CCNGG 4 cut(s) 187, 232, 794, 1192
MunI CAATTG 2 cut(s) 351, 517
Mva1269I GAATGC 1 cut(s) 903
MvaI CCWGG 2 cut(s) 187, 232
MvnI CGCG 1 cut(s) 456
MwoI GCNNNNNNNGC 5 cut(s) 58, 118, 166, 291, 1064
NaeI GCCGGC 1 cut(s) 472
NciI CCSGG 2 cut(s) 794, 1192
NdeII GATC 5 cut(s) 44, 262, 356, 583, 606
NgoMIV GCCGGC 1 cut(s) 470
NlaIII CATG 5 cut(s) 169, 206, 506, 678, 1119
NlaIV GGNNCC 3 cut(s) 159, 302, 1206
NmuCI GTSAC 2 cut(s) 250, 521
NsiI ATGCAT 1 cut(s) 496
NspI RCATGY 3 cut(s) 169, 506, 678
PaeR7I CTCGAG 1 cut(s) 926
PagI TCATGA 1 cut(s) 202
PcsI WCGNNNNNNNCGW 1 cut(s) 53
PctI GAATGC 1 cut(s) 903
PdiI GCCGGC 1 cut(s) 472
PfeI GAWTC 2 cut(s) 342, 569
PkrI GCNGC 8 cut(s) 184, 443, 476, 635, 791, 902, 950, 970
Ple19I CGATCG 1 cut(s) 47
PleI GAGTC 4 cut(s) 75, 251, 739, 881
PmaCI CACGTG 1 cut(s) 152
PmlI CACGTG 1 cut(s) 152
PpsI GAGTC 4 cut(s) 75, 251, 739, 881
Ppu21I YACGTR 1 cut(s) 152
PsiI TTATAA 1 cut(s) 485
Psp124BI GAGCTC 1 cut(s) 210
Psp6I CCWGG 2 cut(s) 185, 230
PspCI CACGTG 1 cut(s) 152
PspGI CCWGG 2 cut(s) 185, 230
PspN4I GGNNCC 3 cut(s) 159, 302, 1206
PspPI GGNCC 1 cut(s) 158
PspXI VCTCGAGB 1 cut(s) 926
PstI CTGCAG 1 cut(s) 479
PstNI CAGNNNCTG 1 cut(s) 968
PsuI RGATCY 1 cut(s) 356
PvuI CGATCG 1 cut(s) 47
PvuII CAGCTG 1 cut(s) 428
RsaI GTAC 1 cut(s) 556
RsaNI GTAC 1 cut(s) 555
SacI GAGCTC 1 cut(s) 210
SaqAI TTAA 6 cut(s) 407, 776, 875, 983, 1016, 1025
SatI GCNGC 8 cut(s) 183, 442, 475, 634, 790, 901, 949, 969
Sau3AI GATC 5 cut(s) 44, 262, 356, 583, 606
Sau96I GGNCC 1 cut(s) 158
SchI GAGTC 4 cut(s) 76, 251, 740, 881
ScrFI CCNGG 4 cut(s) 187, 232, 794, 1192
SduI GDGCHC 2 cut(s) 128, 210
SfaNI GCATC 7 cut(s) 21, 325, 472, 481, 818, 955, 1080
SfcI CTRYAG 2 cut(s) 374, 475
Sfr274I CTCGAG 1 cut(s) 926
SlaI CTCGAG 1 cut(s) 926
SmlI CTYRAG 5 cut(s) 295, 689, 698, 848, 926
SmoI CTYRAG 5 cut(s) 295, 689, 698, 848, 926
Sse9I AATT 4 cut(s) 351, 517, 817, 1125
SsiI CCGC 7 cut(s) 6, 73, 147, 155, 172, 183, 196
SspMI CTAG 2 cut(s) 383, 843
SstI GAGCTC 1 cut(s) 210
StyD4I CCNGG 4 cut(s) 185, 230, 792, 1190
StyI CCWWGG 3 cut(s) 547, 669, 744
TaaI ACNGT 3 cut(s) 320, 378, 713
TaiI ACGT 1 cut(s) 154
TaqI TCGA 4 cut(s) 43, 210, 609, 927
TasI AATT 4 cut(s) 351, 517, 817, 1125
TatI WGTACW 1 cut(s) 554
TauI GCSGC 1 cut(s) 185
TfiI GAWTC 2 cut(s) 342, 569
Tru1I TTAA 6 cut(s) 407, 776, 875, 983, 1016, 1025
Tru9I TTAA 6 cut(s) 407, 776, 875, 983, 1016, 1025
TscAI CASTG 6 cut(s) 30, 133, 465, 667, 920, 1057
TseFI GTSAC 2 cut(s) 250, 521
TseI GCWGC 7 cut(s) 441, 474, 633, 789, 900, 948, 968
Tsp45I GTSAC 2 cut(s) 250, 521
TspDTI ATGAA 3 cut(s) 191, 357, 669
TspGWI ACGGA 3 cut(s) 259, 347, 874
TspRI CASTG 6 cut(s) 30, 133, 465, 667, 920, 1057
XapI RAATTY 1 cut(s) 817
XbaI TCTAGA 1 cut(s) 382
XceI RCATGY 3 cut(s) 169, 506, 678
XhoI CTCGAG 1 cut(s) 926
XspI CTAG 2 cut(s) 383, 843
Zsp2I ATGCAT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.