FvH4_5g00120

36.4 kDa proline-rich

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015789
Physical Location & Seq
Forward (+)
69528 .. 70934
1407 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g00120.t1

Sequence Viewer

Length: 681 bp
ATGATGGATCCAAAGTCTACTGCCTTGTTCTTCATCTTCTTGATTTGCATGGTAATTGCGTTGCCACCAGTTCAAGCTTGTGCCCCTTGTACTCAACCTCATCCACCAATACCACCTAAACATCATCCAGGCCATTCCAAAATTCCTCCCCACCCTAAACACCCACCACACCACGGAGGTGGAGGCGGCGGAGGAGGAGGAGGAGGTGGTGGTGGTGGCGGAGGCGGAAGTGGTGGTCACCCGAAAGTTCCCCCGCCATACAAAAACCCGCCAGTTGTATCTCCCCCAATTATAACACCCCCTATTAATAAACCTCCGGTGACAAACCCACCTTACATCCCTCCTCCAACGCCTCCTTGTGCCGGATGCGGTGGAGGAGGTGGCTCGGGTAAACATCCTCCGCCTTCTACTACACCGACTACTTGTCCTATTGATGCGCTCAAGTTAGGGCTTTGTGTGGATGTACTTGGTGGATTGGTGCATATAGGGTTGGGGAATCCAGTTGAGAATGCTTGCTGTCCGGTCCTCCAAGGGTTGCTTGAGCTTGAAGCAGCGATTTGTCTGTGCACGACTATAAGGCTTAAGCTGCTAAACCTAAACATCTTTATTCCTCTTGCTCTTCAAGCTTTAATCACTTGCGGCATAACTCCTCCTCCTGGTTTCGTATGTCCTCCGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrophob_seed

Protein Analysis

227

Amino Acids

22.64

Weight (kDa)

8.58

Isoelectric Point (pI)

60.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrophob_seed PF14547 141 - 224 1.7e-25 Hydrophobic seed protein
Tryp_alpha_amyl PF00234 142 - 223 1.5e-11 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015789)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62500
fragaria_vesca FvH4_5g00120
malus_domestica MD04G1087700.v1.1
prunus_persica Prupe.5G073000_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0202811
rosa_laevigata RLG00000003570
rosa_multiflora Rmu_sc0002590.1_g000023 Rmu_sc0003429.1_g000016
rosa_roxburghii Rroxscaffold_3G00254240
rosa_rugosa Rorug07G0073200
rosa_samantha Rh7AG201000 Rh7BG201200 Rh7CG212400 Rh7DG207900
rosa_wichuraiana Rw7G017580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 293
AccI GTMKAC 1 cut(s) 17
AclWI GGATC 2 cut(s) 2, 15
AcsI RAATTY 1 cut(s) 141
AfaI GTAC 2 cut(s) 91, 465
AfiI CCNNNNNNNGG 3 cut(s) 173, 362, 656
AflII CTTAAG 1 cut(s) 581
AgsI TTSAA 3 cut(s) 74, 548, 623
AhdI GACNNNNNGTC 1 cut(s) 423
AjnI CCWGG 2 cut(s) 127, 655
AleI CACNNNNGTG 1 cut(s) 177
AluBI AGCT 4 cut(s) 77, 544, 586, 626
AluI AGCT 4 cut(s) 77, 544, 586, 626
Alw21I GWGCWC 1 cut(s) 569
Alw44I GTGCAC 1 cut(s) 565
AlwI GGATC 2 cut(s) 2, 15
Ama87I CYCGRG 1 cut(s) 385
AoxI GGCC 1 cut(s) 130
ApaLI GTGCAC 1 cut(s) 565
ApeKI GCWGC 2 cut(s) 551, 586
ApoI RAATTY 1 cut(s) 141
AseI ATTAAT 1 cut(s) 306
AspLEI GCGC 1 cut(s) 439
AspS9I GGNCC 1 cut(s) 523
AsuHPI GGTGA 2 cut(s) 230, 331
AvaI CYCGRG 1 cut(s) 385
AvaII GGWCC 1 cut(s) 523
BaeGI GKGCMC 2 cut(s) 85, 569
BamHI GGATCC 1 cut(s) 7
Bbv12I GWGCWC 1 cut(s) 569
BbvI GCAGC 2 cut(s) 563, 573
BciT130I CCWGG 2 cut(s) 129, 657
BfrI CTTAAG 1 cut(s) 581
BisI GCNGC 4 cut(s) 187, 552, 587, 640
BlsI GCNGC 4 cut(s) 188, 553, 588, 641
Bme1390I CCNGG 2 cut(s) 129, 657
Bme18I GGWCC 1 cut(s) 523
BmeRI GACNNNNNGTC 1 cut(s) 423
BmeT110I CYCGRG 1 cut(s) 385
BmgT120I GGNCC 1 cut(s) 523
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 2 cut(s) 129, 657
BmsI GCATC 2 cut(s) 356, 424
BpuEI CTTGAG 2 cut(s) 425, 560
BsaJI CCNNGG 2 cut(s) 172, 529
BsaWI WCCGGW 2 cut(s) 316, 520
BsaXI ACNNNNNCTCC 2 cut(s) 637, 667
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 362, 656
Bse1I ACTGG 3 cut(s) 68, 272, 500
BseBI CCWGG 2 cut(s) 129, 657
BseDI CCNNGG 2 cut(s) 172, 529
BseGI GGATG 6 cut(s) 100, 124, 336, 371, 394, 466
BseLI CCNNNNNNNGG 3 cut(s) 173, 362, 656
BseNI ACTGG 3 cut(s) 68, 272, 500
BseRI GAGGAG 8 cut(s) 207, 210, 213, 216, 333, 390, 639, 642
BseSI GKGCMC 2 cut(s) 85, 569
BseXI GCAGC 2 cut(s) 563, 573
BshFI GGCC 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 569
BsiHKCI CYCGRG 1 cut(s) 385
BsiSI CCGG 3 cut(s) 317, 363, 521
BslI CCNNNNNNNGG 3 cut(s) 173, 362, 656
BsmI GAATGC 1 cut(s) 514
BsnI GGCC 1 cut(s) 132
BsoBI CYCGRG 1 cut(s) 385
Bsp1286I GDGCHC 2 cut(s) 85, 569
Bsp143I GATC 1 cut(s) 7
BspANI GGCC 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 9
BspPI GGATC 2 cut(s) 2, 15
BspQI GCTCTTC 1 cut(s) 624
BspTI CTTAAG 1 cut(s) 581
BsrI ACTGG 3 cut(s) 68, 272, 500
BssECI CCNNGG 2 cut(s) 172, 529
BssMI GATC 1 cut(s) 7
BssT1I CCWWGG 1 cut(s) 529
Bst2UI CCWGG 2 cut(s) 129, 657
Bst6I CTCTTC 1 cut(s) 624
BstAFI CTTAAG 1 cut(s) 581
BstC8I GCNNGC 1 cut(s) 514
BstDSI CCRYGG 1 cut(s) 172
BstEII GGTNACC 1 cut(s) 236
BstF5I GGATG 6 cut(s) 100, 124, 336, 371, 394, 466
BstHHI GCGC 1 cut(s) 439
BstKTI GATC 1 cut(s) 10
BstMBI GATC 1 cut(s) 7
BstMWI GCNNNNNNNGC 2 cut(s) 586, 623
BstNI CCWGG 2 cut(s) 129, 657
BstPI GGTNACC 1 cut(s) 236
BstSCI CCNGG 2 cut(s) 127, 655
BstSLI GKGCMC 2 cut(s) 85, 569
BstV1I GCAGC 2 cut(s) 563, 573
BstX2I RGATCY 1 cut(s) 7
BstXI CCANNNNNNTGG 1 cut(s) 179
BstYI RGATCY 1 cut(s) 7
BsuRI GGCC 1 cut(s) 132
BtgI CCRYGG 1 cut(s) 172
BtsCI GGATG 6 cut(s) 100, 124, 336, 371, 394, 466
Cac8I GCNNGC 1 cut(s) 514
CfoI GCGC 1 cut(s) 439
Cfr13I GGNCC 1 cut(s) 523
Csp6I GTAC 2 cut(s) 90, 464
CviAII CATG 1 cut(s) 49
CviJI RGCY 8 cut(s) 77, 132, 384, 451, 544, 580, 586, 626
CviKI_1 RGCY 8 cut(s) 77, 132, 384, 451, 544, 580, 586, 626
CviQI GTAC 2 cut(s) 90, 464
DpnI GATC 1 cut(s) 9
DpnII GATC 1 cut(s) 7
DriI GACNNNNNGTC 1 cut(s) 423
Eam1104I CTCTTC 1 cut(s) 624
Eam1105I GACNNNNNGTC 1 cut(s) 423
EarI CTCTTC 1 cut(s) 624
EciI GGCGGA 4 cut(s) 204, 234, 240, 390
Eco130I CCWWGG 1 cut(s) 529
Eco47I GGWCC 1 cut(s) 523
Eco88I CYCGRG 1 cut(s) 385
Eco91I GGTNACC 1 cut(s) 236
EcoO65I GGTNACC 1 cut(s) 236
EcoRII CCWGG 2 cut(s) 127, 655
EcoT14I CCWWGG 1 cut(s) 529
ErhI CCWWGG 1 cut(s) 529
FaeI CATG 1 cut(s) 52
FaiI YATR 8 cut(s) 50, 259, 293, 483, 485, 575, 644, 667
FalI AAGNNNNNCTT 2 cut(s) 522, 554
FatI CATG 1 cut(s) 48
FauI CCCGC 2 cut(s) 261, 276
FblI GTMKAC 1 cut(s) 17
Fnu4HI GCNGC 4 cut(s) 187, 552, 587, 640
FokI GGATG 6 cut(s) 87, 111, 323, 378, 381, 473
Fsp4HI GCNGC 4 cut(s) 187, 552, 587, 640
GlaI GCGC 1 cut(s) 438
GluI GCNGC 4 cut(s) 187, 552, 587, 640
HaeIII GGCC 1 cut(s) 132
HapII CCGG 3 cut(s) 317, 363, 521
HhaI GCGC 1 cut(s) 439
Hin1II CATG 1 cut(s) 52
Hin6I GCGC 1 cut(s) 437
HinP1I GCGC 1 cut(s) 437
HindIII AAGCTT 2 cut(s) 75, 624
HinfI GANTC 1 cut(s) 496
HpaII CCGG 3 cut(s) 317, 363, 521
HphI GGTGA 2 cut(s) 230, 331
Hpy166II GTNNAC 3 cut(s) 18, 392, 567
Hpy188III TCNNGA 1 cut(s) 40
Hpy8I GTNNAC 3 cut(s) 18, 392, 567
HpyAV CCTTC 1 cut(s) 414
HpyCH4V TGCA 3 cut(s) 48, 481, 567
HpyF10VI GCNNNNNNNGC 2 cut(s) 586, 623
Hsp92II CATG 1 cut(s) 52
HspAI GCGC 1 cut(s) 437
Kzo9I GATC 1 cut(s) 7
LguI GCTCTTC 1 cut(s) 624
Lsp1109I GCAGC 2 cut(s) 563, 573
LweI GCATC 2 cut(s) 356, 424
MaeIII GTNAC 2 cut(s) 236, 319
MalI GATC 1 cut(s) 9
MboI GATC 1 cut(s) 7
MboII GAAGA 3 cut(s) 22, 28, 611
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 2 cut(s) 85, 569
MluCI AATT 3 cut(s) 54, 141, 288
MmeI TCCRAC 1 cut(s) 371
MseI TTAA 4 cut(s) 306, 582, 629, 679
MslI CAYNNNNRTG 1 cut(s) 177
MspCI CTTAAG 1 cut(s) 581
MspI CCGG 3 cut(s) 317, 363, 521
MspR9I CCNGG 2 cut(s) 129, 657
Mva1269I GAATGC 1 cut(s) 514
MvaI CCWGG 2 cut(s) 129, 657
MwoI GCNNNNNNNGC 2 cut(s) 586, 623
NdeII GATC 1 cut(s) 7
NlaIII CATG 1 cut(s) 52
NlaIV GGNNCC 1 cut(s) 9
NmuCI GTSAC 2 cut(s) 236, 319
OliI CACNNNNGTG 1 cut(s) 177
PciSI GCTCTTC 1 cut(s) 624
PctI GAATGC 1 cut(s) 514
PfeI GAWTC 1 cut(s) 496
PkrI GCNGC 4 cut(s) 188, 553, 588, 641
PshBI ATTAAT 1 cut(s) 306
PsiI TTATAA 1 cut(s) 293
Psp6I CCWGG 2 cut(s) 127, 655
PspEI GGTNACC 1 cut(s) 236
PspGI CCWGG 2 cut(s) 127, 655
PspN4I GGNNCC 1 cut(s) 9
PspPI GGNCC 1 cut(s) 523
PsuI RGATCY 1 cut(s) 7
RsaI GTAC 2 cut(s) 91, 465
RsaNI GTAC 2 cut(s) 90, 464
RseI CAYNNNNRTG 1 cut(s) 177
SapI GCTCTTC 1 cut(s) 624
SaqAI TTAA 4 cut(s) 306, 582, 629, 679
SatI GCNGC 4 cut(s) 187, 552, 587, 640
Sau3AI GATC 1 cut(s) 7
Sau96I GGNCC 1 cut(s) 523
ScrFI CCNGG 2 cut(s) 129, 657
SduI GDGCHC 2 cut(s) 85, 569
SfaNI GCATC 2 cut(s) 356, 424
SinI GGWCC 1 cut(s) 523
SmiMI CAYNNNNRTG 1 cut(s) 177
SmlI CTYRAG 3 cut(s) 440, 539, 581
SmoI CTYRAG 3 cut(s) 440, 539, 581
Sse9I AATT 3 cut(s) 54, 141, 288
StyD4I CCNGG 2 cut(s) 127, 655
StyI CCWWGG 1 cut(s) 529
TasI AATT 3 cut(s) 54, 141, 288
TatI WGTACW 2 cut(s) 89, 463
TauI GCSGC 2 cut(s) 189, 642
TfiI GAWTC 1 cut(s) 496
Tru1I TTAA 4 cut(s) 306, 582, 629, 679
Tru9I TTAA 4 cut(s) 306, 582, 629, 679
TseFI GTSAC 2 cut(s) 236, 319
TseI GCWGC 2 cut(s) 551, 586
Tsp45I GTSAC 2 cut(s) 236, 319
TspDTI ATGAA 1 cut(s) 22
TspGWI ACGGA 1 cut(s) 189
Vha464I CTTAAG 1 cut(s) 581
VneI GTGCAC 1 cut(s) 565
VpaK11BI GGWCC 1 cut(s) 523
VspI ATTAAT 1 cut(s) 306
XapI RAATTY 1 cut(s) 141
XmiI GTMKAC 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.