Rroxscaffold_3G00254240

36.4 kDa proline-rich

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0015789
Physical Location & Seq
Forward (+)
48395760 .. 48396463
704 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00254240.1

Sequence Viewer

Length: 666 bp
ATGATGGGTTCAAAGTCTACTGCCCTGTTCTTCATCTTCTTGATTTGCATGGTAATTGCGTTGCCACCAATTCAAGCTTGTGCTCCTTGTACTCAGCCTCATCCACCAAAACCGCCTAAACATCATCCAGGCCATTCCAAAATTCCTCCCCACGCCAAACACCCACCACACCAAGGAGGTGGAGGCGGCGGCGGCAAAGGAGGTGGAGGTGGAATTGGTGTCGTATCTCCACCAATAATAACACCCCCTATTACTAAACCTCCTGTGACAATCCCACCTCCTTCCCCCTCGTACCCACCTTACATCCCTCCTCCAACGCCTCCTTGTGCTGGATGCGGTGGAGGAGGTGGCGGAGGAGGAGGAGGTGGCTCGGGTAAACATCCTCCGCCTTCTACTAAACCAGCTACTTGTCCTATTGATGCGCTGAAGCTAGGGCTTTGTGTGGATGTGCTGGGTGGTTTGGTGCATATCGGGTTGGGGAATCCAGTTGAGAATGCTTGCTGTCCCGTGCTCCAAGGGTTGCTTGAGCTTGAAGCAGCGATTTGTCTTTGCACGGCCATAAGGCTTAAGCTGCTAAACCTAAACATCTTCATTCCTCTTGCTCTTCAAGCTTTGATCACTTGCGGCATAACTCCTCCTCCTGGTTTTGTATGTCCGCCTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrophob_seed

Protein Analysis

221

Amino Acids

21.99

Weight (kDa)

8.83

Isoelectric Point (pI)

61.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrophob_seed PF14547 136 - 219 2.3e-25 Hydrophobic seed protein
Tryp_alpha_amyl PF00234 137 - 218 7.9e-12 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015789)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62500
fragaria_vesca FvH4_5g00120
malus_domestica MD04G1087700.v1.1
prunus_persica Prupe.5G073000_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0202811
rosa_laevigata RLG00000003570
rosa_multiflora Rmu_sc0002590.1_g000023 Rmu_sc0003429.1_g000016
rosa_roxburghii Rroxscaffold_3G00254240
rosa_rugosa Rorug07G0073200
rosa_samantha Rh7AG201000 Rh7BG201200 Rh7CG212400 Rh7DG207900
rosa_wichuraiana Rw7G017580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 17
AciI CCGC 9 cut(s) 113, 186, 189, 192, 336, 351, 386, 624, 656
AcoI YGGCCR 1 cut(s) 555
AcsI RAATTY 1 cut(s) 141
AcuI CTGAAG 1 cut(s) 446
AfaI GTAC 2 cut(s) 91, 293
AfiI CCNNNNNNNGG 3 cut(s) 173, 329, 641
AflII CTTAAG 1 cut(s) 566
AgsI TTSAA 4 cut(s) 12, 74, 533, 608
AjnI CCWGG 2 cut(s) 127, 640
AluBI AGCT 6 cut(s) 77, 404, 430, 529, 571, 611
AluI AGCT 6 cut(s) 77, 404, 430, 529, 571, 611
Alw21I GWGCWC 2 cut(s) 85, 513
Ama87I CYCGRG 1 cut(s) 370
AoxI GGCC 2 cut(s) 130, 555
ApeKI GCWGC 2 cut(s) 536, 571
ApoI RAATTY 1 cut(s) 141
AspLEI GCGC 1 cut(s) 424
AvaI CYCGRG 1 cut(s) 370
Bbv12I GWGCWC 2 cut(s) 85, 513
BbvI GCAGC 2 cut(s) 548, 558
BceAI ACGGC 1 cut(s) 570
BciT130I CCWGG 2 cut(s) 129, 642
BclI TGATCA 1 cut(s) 615
BfaI CTAG 1 cut(s) 431
BfrI CTTAAG 1 cut(s) 566
BisI GCNGC 6 cut(s) 187, 190, 193, 537, 572, 625
BlsI GCNGC 6 cut(s) 188, 191, 194, 538, 573, 626
Bme1390I CCNGG 2 cut(s) 129, 642
BmeT110I CYCGRG 1 cut(s) 370
BmrFI CCNGG 2 cut(s) 129, 642
BmsI GCATC 2 cut(s) 323, 409
BpuEI CTTGAG 1 cut(s) 545
BsaJI CCNNGG 2 cut(s) 172, 514
BsaXI ACNNNNNCTCC 4 cut(s) 244, 274, 622, 652
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 329, 641
Bse1I ACTGG 1 cut(s) 485
BseBI CCWGG 2 cut(s) 129, 642
BseDI CCNNGG 2 cut(s) 172, 514
BseGI GGATG 6 cut(s) 100, 124, 303, 338, 379, 451
BseLI CCNNNNNNNGG 3 cut(s) 173, 329, 641
BseMII CTCAG 1 cut(s) 107
BseNI ACTGG 1 cut(s) 485
BseRI GAGGAG 7 cut(s) 300, 357, 369, 372, 375, 624, 627
BseXI GCAGC 2 cut(s) 548, 558
BseYI CCCAGC 1 cut(s) 451
BshFI GGCC 2 cut(s) 132, 557
BsiHKAI GWGCWC 2 cut(s) 85, 513
BsiHKCI CYCGRG 1 cut(s) 370
BslFI GGGAC 1 cut(s) 489
BslI CCNNNNNNNGG 3 cut(s) 173, 329, 641
BsmFI GGGAC 1 cut(s) 489
BsmI GAATGC 1 cut(s) 499
BsnI GGCC 2 cut(s) 132, 557
BsoBI CYCGRG 1 cut(s) 370
Bsp1286I GDGCHC 2 cut(s) 85, 513
Bsp143I GATC 1 cut(s) 615
BspACI CCGC 9 cut(s) 113, 186, 189, 192, 336, 351, 386, 624, 656
BspANI GGCC 2 cut(s) 132, 557
BspCNI CTCAG 1 cut(s) 106
BspQI GCTCTTC 1 cut(s) 609
BspTI CTTAAG 1 cut(s) 566
BsrI ACTGG 1 cut(s) 485
BssECI CCNNGG 2 cut(s) 172, 514
BssMI GATC 1 cut(s) 615
BssT1I CCWWGG 2 cut(s) 172, 514
Bst2UI CCWGG 2 cut(s) 129, 642
Bst6I CTCTTC 1 cut(s) 609
BstAFI CTTAAG 1 cut(s) 566
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 1 cut(s) 93
BstF5I GGATG 6 cut(s) 100, 124, 303, 338, 379, 451
BstHHI GCGC 1 cut(s) 424
BstKTI GATC 1 cut(s) 618
BstMBI GATC 1 cut(s) 615
BstMWI GCNNNNNNNGC 3 cut(s) 192, 571, 608
BstNI CCWGG 2 cut(s) 129, 642
BstSCI CCNGG 2 cut(s) 127, 640
BstV1I GCAGC 2 cut(s) 548, 558
BstXI CCANNNNNNTGG 1 cut(s) 179
BsuRI GGCC 2 cut(s) 132, 557
BtsCI GGATG 6 cut(s) 100, 124, 303, 338, 379, 451
Cac8I GCNNGC 1 cut(s) 499
CfoI GCGC 1 cut(s) 424
Csp6I GTAC 2 cut(s) 90, 292
CspCI CAANNNNNGTGG 2 cut(s) 184, 219
CviAII CATG 1 cut(s) 49
CviQI GTAC 2 cut(s) 90, 292
DdeI CTNAG 1 cut(s) 93
DpnI GATC 1 cut(s) 617
DpnII GATC 1 cut(s) 615
EaeI YGGCCR 1 cut(s) 555
Eam1104I CTCTTC 1 cut(s) 609
EarI CTCTTC 1 cut(s) 609
EciI GGCGGA 3 cut(s) 366, 375, 645
Eco130I CCWWGG 2 cut(s) 172, 514
Eco57I CTGAAG 1 cut(s) 446
Eco88I CYCGRG 1 cut(s) 370
EcoRII CCWGG 2 cut(s) 127, 640
EcoT14I CCWWGG 2 cut(s) 172, 514
ErhI CCWWGG 2 cut(s) 172, 514
FaeI CATG 1 cut(s) 52
FaiI YATR 5 cut(s) 50, 468, 560, 629, 652
FalI AAGNNNNNCTT 2 cut(s) 507, 539
FaqI GGGAC 1 cut(s) 489
FatI CATG 1 cut(s) 48
FbaI TGATCA 1 cut(s) 615
FblI GTMKAC 1 cut(s) 17
Fnu4HI GCNGC 6 cut(s) 187, 190, 193, 537, 572, 625
FokI GGATG 6 cut(s) 87, 111, 290, 345, 366, 458
Fsp4HI GCNGC 6 cut(s) 187, 190, 193, 537, 572, 625
FspBI CTAG 1 cut(s) 431
GlaI GCGC 1 cut(s) 423
GluI GCNGC 6 cut(s) 187, 190, 193, 537, 572, 625
GsaI CCCAGC 1 cut(s) 455
HaeIII GGCC 2 cut(s) 132, 557
HhaI GCGC 1 cut(s) 424
Hin1II CATG 1 cut(s) 52
Hin6I GCGC 1 cut(s) 422
HinP1I GCGC 1 cut(s) 422
HindIII AAGCTT 2 cut(s) 75, 609
HinfI GANTC 1 cut(s) 481
Hpy166II GTNNAC 2 cut(s) 18, 377
Hpy188III TCNNGA 1 cut(s) 40
Hpy8I GTNNAC 2 cut(s) 18, 377
HpyAV CCTTC 2 cut(s) 291, 399
HpyCH4V TGCA 3 cut(s) 48, 466, 552
HpyF10VI GCNNNNNNNGC 3 cut(s) 192, 571, 608
HpyF3I CTNAG 1 cut(s) 93
Hsp92II CATG 1 cut(s) 52
HspAI GCGC 1 cut(s) 422
Ksp22I TGATCA 1 cut(s) 615
Kzo9I GATC 1 cut(s) 615
LguI GCTCTTC 1 cut(s) 609
LmnI GCTCC 2 cut(s) 88, 516
Lsp1109I GCAGC 2 cut(s) 548, 558
LweI GCATC 2 cut(s) 323, 409
MaeI CTAG 1 cut(s) 431
MaeIII GTNAC 1 cut(s) 265
MalI GATC 1 cut(s) 617
MboI GATC 1 cut(s) 615
MboII GAAGA 4 cut(s) 22, 28, 580, 596
MhlI GDGCHC 2 cut(s) 85, 513
MluCI AATT 4 cut(s) 54, 69, 141, 213
MmeI TCCRAC 1 cut(s) 338
MseI TTAA 2 cut(s) 567, 664
MspCI CTTAAG 1 cut(s) 566
MspR9I CCNGG 2 cut(s) 129, 642
Mva1269I GAATGC 1 cut(s) 499
MvaI CCWGG 2 cut(s) 129, 642
MwoI GCNNNNNNNGC 3 cut(s) 192, 571, 608
NdeII GATC 1 cut(s) 615
NlaIII CATG 1 cut(s) 52
NmuCI GTSAC 1 cut(s) 265
PciSI GCTCTTC 1 cut(s) 609
PctI GAATGC 1 cut(s) 499
PfeI GAWTC 1 cut(s) 481
PkrI GCNGC 6 cut(s) 188, 191, 194, 538, 573, 626
Psp6I CCWGG 2 cut(s) 127, 640
PspFI CCCAGC 1 cut(s) 451
PspGI CCWGG 2 cut(s) 127, 640
RsaI GTAC 2 cut(s) 91, 293
RsaNI GTAC 2 cut(s) 90, 292
SapI GCTCTTC 1 cut(s) 609
SaqAI TTAA 2 cut(s) 567, 664
SatI GCNGC 6 cut(s) 187, 190, 193, 537, 572, 625
Sau3AI GATC 1 cut(s) 615
ScrFI CCNGG 2 cut(s) 129, 642
SduI GDGCHC 2 cut(s) 85, 513
SfaNI GCATC 2 cut(s) 323, 409
SmlI CTYRAG 2 cut(s) 524, 566
SmoI CTYRAG 2 cut(s) 524, 566
Sse9I AATT 4 cut(s) 54, 69, 141, 213
SsiI CCGC 9 cut(s) 113, 186, 189, 192, 336, 351, 386, 624, 656
SspMI CTAG 1 cut(s) 431
StyD4I CCNGG 2 cut(s) 127, 640
StyI CCWWGG 2 cut(s) 172, 514
TasI AATT 4 cut(s) 54, 69, 141, 213
TatI WGTACW 1 cut(s) 89
TauI GCSGC 4 cut(s) 189, 192, 195, 627
TfiI GAWTC 1 cut(s) 481
Tru1I TTAA 2 cut(s) 567, 664
Tru9I TTAA 2 cut(s) 567, 664
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 2 cut(s) 536, 571
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 2 cut(s) 22, 580
Vha464I CTTAAG 1 cut(s) 566
XapI RAATTY 1 cut(s) 141
XmiI GTMKAC 1 cut(s) 17
XspI CTAG 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.