FvH4_5g04510

Leucine-rich repeats, outliers

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010274
Physical Location & Seq
Forward (+)
2654713 .. 2658117
3405 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g04510.t1

Sequence Viewer

Length: 1836 bp
ATGGAGACCCAACTGGTGGAGCTTTGCTTAAACTCCGCGTGTGAAAGCAGAGACAGCGTCGAGAAATGGCGGCGGCAGCGGCGGAGTCTAGAGCACTTACCTTCTCAGCTCGCCGACTCTCTCCTCCGTCGCCTCATCTCTCGCCGCCTCATCTTCCCCTCTTTGCTCGAGGTGTTCAAAAACAGTGCTGAGGAGATTGATCTGAGAGGCGACACCAACGTTGACGCTGAGTGGTTTGCATACATTGGAGCATTTAAACACTTGCGGAGTTTGAATGTCTCTGACTGCCGTAGACTCACAAGCTCAGCTCTTTGGGCTTTAGCAGGGATGGAGAGTTTATGGGAGCTGGATCTTTCGAGATGTCCCAAGGTTAATGATGGTGGCATTAGACATCTTTTATCTGTGACAAGTTTGGAGAAGTTGTGTATTTCGGAGACGGGTGTGTCTGCTAAAGGGGTTATGCTTCTCTCTACGCTTGGAAACTTGTGTGTTCTCAACTTGGGTGGTTTGCCTGTCACTGATCAAGCACTGAGTTCTCTCCAGGTACTTAATAAACTCCAGTACCTTGACCTTTGGGGGAGTAAGATATCTGACAAGGGTGCTGCTCTACTTCATATGTTCCCTAGGTTGACTTTCCTCAATCTAGCTTGGACAAGCGTCACGAAGTTGCCAAATCTGTTAACCCTTGAATGCTTAAACATGAGTCACTGTACAATAAATTCTCTACTTGAAGGAGATAATGGTAAAGCTCCTTTAGTCAAGCTTACTGTTTCTGGAGCTACATTTGGAGAAGAGGTTGCTAATTTCCATTATATAGAAACAACCATATTAACCTTTTTGGATCTGTCCAAGTCTACGATTCAAGACTTCAGCTTCTTATCTCATCTAAATGCGCTGAAACACTTGGATCTCAGCTCAACCGCCATAGGGGATGATTCACTTGAACTTGTTGCATGTATTGGAGTGAATTTGAAACATCTAAATCTTAACAAAACCAAAGTAAGCTCTGCAGGAGTTGGAAATTTATCAGGGCATGTACCTAATCTTGAGGTGTTTTCTATATCTCATACATCAGTAGATGATGTTGCCATATCTTACATTGGGATGATGCCTTCTGTGAAGGATGTTGACTTGAGCAATACCAACATTAAAGGTGTCATTTACCAAGCGGAGCATGTACCTGAGCCTGAGCATGTACCTGAGCATGAGCATGAGCTTGAGTCTGAGCCTGAGCTTGAGCCAGATTCAGTCCTCTCACTGTCAGCACTTCAAAGTCTCAGGCATCTGGAAAGGTTGAATTTGGCAGATACACAAGTTATGGATGCAGCTCTATACCCTTTAACAAGCTTTCTGGAGTTAAGGCATTTATCTCTTAAAAACGCTTCTCTTACTGACTTCTCCCTTAACTATGTGTCATCCATATCAAAGCTGACAAATGTCTGCATTAATGATGGTGTTCTGACAAATTCTGGGCTCATTTCATTCAAACCCCCAGCAACTCTAAGAATGATGGACCTGAGGGGTTGTTGGCTCTTAACTGTGGATGCTATCACATCCTTTTCTAAAACTCACCCCCAGATTGAAGTATGGCATGATCTTATTCACATCTCACCACCAGACCTGACTATTTTTAGTGCTGCACCTTCTTCAAGATCAACTTTGAAGACTTCACAAGTGAAGAAGGAGAAGCATGAAAGTATTCCCATGTTGGAATGTTTTTTAGATCAAAGATTGAAGTATAGCAGAGAAGAGCTTCTTTCTATGCGGTTCTCGTCTGTTTCCCTTGCAGCTCCTATTGATAGAGACATTGGAATATGCAACACACAATTGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_6 LRR_8

Protein Analysis

612

Amino Acids

67.62

Weight (kDa)

5.67

Isoelectric Point (pI)

42.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 66 - 204 2.1e-06 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 442
AccB7I CCANNNNNTGG 1 cut(s) 16
AccI GTMKAC 2 cut(s) 292, 854
AccII CGCG 1 cut(s) 38
AclI AACGTT 1 cut(s) 219
AclWI GGATC 3 cut(s) 357, 849, 915
AcsI RAATTY 5 cut(s) 718, 967, 1021, 1297, 1465
AcuI CTGAAG 1 cut(s) 853
AfaI GTAC 6 cut(s) 546, 563, 712, 1038, 1179, 1197
AfiI CCNNNNNNNGG 2 cut(s) 16, 927
AjnI CCWGG 1 cut(s) 540
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 5 cut(s) 45, 283, 428, 1280, 1797
AlwI GGATC 3 cut(s) 357, 849, 915
Ama87I CYCGRG 1 cut(s) 167
ApeKI GCWGC 5 cut(s) 76, 602, 1325, 1637, 1787
ApoI RAATTY 5 cut(s) 718, 967, 1021, 1297, 1465
AseI ATTAAT 1 cut(s) 1446
Asp700I GAANNNNTTC 2 cut(s) 1698, 1752
AspA2I CCTAGG 1 cut(s) 623
AspLEI GCGC 1 cut(s) 895
AspS9I GGNCC 1 cut(s) 1513
AsuHPI GGTGA 2 cut(s) 1562, 1602
AvaI CYCGRG 1 cut(s) 167
AvaII GGWCC 1 cut(s) 1513
AvrII CCTAGG 1 cut(s) 623
AxyI CCTNAGG 1 cut(s) 1517
BaeI ACNNNNGTAYC 4 cut(s) 545, 578, 1299, 1332
BanII GRGCYC 1 cut(s) 1476
BbsI GAAGAC 1 cut(s) 1670
Bbv12I GWGCWC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 189
BbvI GCAGC 5 cut(s) 88, 589, 1337, 1624, 1799
BccI CCATC 4 cut(s) 322, 371, 1445, 1504
BceAI ACGGC 1 cut(s) 273
BciT130I CCWGG 1 cut(s) 542
BclI TGATCA 1 cut(s) 520
BcoDI GTCTC 5 cut(s) 45, 283, 428, 1280, 1797
BfaI CTAG 3 cut(s) 89, 624, 644
BfmI CTRYAG 1 cut(s) 1008
BisI GCNGC 9 cut(s) 71, 74, 77, 80, 145, 603, 1326, 1638, 1788
BlnI CCTAGG 1 cut(s) 623
BlpI GCTNAGC 1 cut(s) 304
BlsI GCNGC 9 cut(s) 72, 75, 78, 81, 146, 604, 1327, 1639, 1789
Bme1390I CCNGG 1 cut(s) 542
Bme18I GGWCC 1 cut(s) 1513
BmeT110I CYCGRG 1 cut(s) 167
BmgT120I GGNCC 1 cut(s) 1513
BmrFI CCNGG 1 cut(s) 542
BmsI GCATC 4 cut(s) 1098, 1291, 1312, 1534
BoxI GACNNNNGTC 2 cut(s) 656, 1436
BpiI GAAGAC 1 cut(s) 1670
BpmI CTGGAG 4 cut(s) 524, 542, 795, 1373
Bpu10I CCTNAGC 5 cut(s) 189, 1182, 1188, 1200, 1230
Bpu1102I GCTNAGC 1 cut(s) 304
BpuEI CTTGAG 4 cut(s) 1067, 1153, 1238, 1256
BsaJI CCNNGG 2 cut(s) 366, 623
BsaXI ACNNNNNCTCC 4 cut(s) 407, 437, 780, 810
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 927
Bse1I ACTGG 2 cut(s) 18, 559
Bse21I CCTNAGG 1 cut(s) 1517
BseBI CCWGG 1 cut(s) 542
BseDI CCNNGG 2 cut(s) 366, 623
BseGI GGATG 8 cut(s) 333, 937, 1110, 1129, 1327, 1415, 1549, 1553
BseLI CCNNNNNNNGG 2 cut(s) 16, 927
BseNI ACTGG 2 cut(s) 18, 559
BseRI GAGGAG 2 cut(s) 113, 206
BseXI GCAGC 5 cut(s) 88, 589, 1337, 1624, 1799
BseYI CCCAGC 1 cut(s) 1492
BsgI GTGCAG 1 cut(s) 1623
Bsh1236I CGCG 1 cut(s) 38
BsiHKAI GWGCWC 1 cut(s) 96
BsiHKCI CYCGRG 1 cut(s) 167
BslFI GGGAC 1 cut(s) 348
BslI CCNNNNNNNGG 2 cut(s) 16, 927
BsmAI GTCTC 5 cut(s) 45, 283, 428, 1280, 1797
BsmBI CGTCTC 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 348
BsmI GAATGC 1 cut(s) 695
BsoBI CYCGRG 1 cut(s) 167
Bsp1286I GDGCHC 2 cut(s) 96, 1476
Bsp1407I TGTACA 1 cut(s) 710
Bsp143I GATC 8 cut(s) 199, 349, 520, 841, 907, 1594, 1652, 1723
Bsp1720I GCTNAGC 1 cut(s) 304
BspFNI CGCG 1 cut(s) 38
BspMAI CTGCAG 1 cut(s) 1012
BspPI GGATC 3 cut(s) 357, 849, 915
BspQI GCTCTTC 1 cut(s) 1743
BsrGI TGTACA 1 cut(s) 710
BsrI ACTGG 2 cut(s) 18, 559
BssECI CCNNGG 2 cut(s) 366, 623
BssMI GATC 8 cut(s) 199, 349, 520, 841, 907, 1594, 1652, 1723
BssT1I CCWWGG 2 cut(s) 366, 623
Bst2UI CCWGG 1 cut(s) 542
Bst4CI ACNGT 5 cut(s) 185, 710, 769, 1260, 1540
Bst6I CTCTTC 2 cut(s) 786, 1743
BstAUI TGTACA 1 cut(s) 710
BstC8I GCNNGC 1 cut(s) 111
BstF5I GGATG 8 cut(s) 333, 937, 1110, 1129, 1327, 1415, 1549, 1553
BstFNI CGCG 1 cut(s) 38
BstHHI GCGC 1 cut(s) 895
BstKTI GATC 8 cut(s) 202, 352, 523, 844, 910, 1597, 1655, 1726
BstMAI GTCTC 5 cut(s) 45, 283, 428, 1280, 1797
BstMBI GATC 8 cut(s) 199, 349, 520, 841, 907, 1594, 1652, 1723
BstMWI GCNNNNNNNGC 4 cut(s) 54, 76, 79, 314
BstNI CCWGG 1 cut(s) 542
BstNSI RCATGY 4 cut(s) 957, 1037, 1178, 1196
BstPAI GACNNNNGTC 2 cut(s) 656, 1436
BstSCI CCNGG 1 cut(s) 540
BstSFI CTRYAG 1 cut(s) 1008
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 5 cut(s) 88, 589, 1337, 1624, 1799
BstV2I GAAGAC 1 cut(s) 1670
BstX2I RGATCY 3 cut(s) 349, 841, 907
BstYI RGATCY 3 cut(s) 349, 841, 907
Bsu36I CCTNAGG 1 cut(s) 1517
BtsCI GGATG 8 cut(s) 333, 937, 1110, 1129, 1327, 1415, 1549, 1553
BtsIMutI CAGTG 5 cut(s) 190, 516, 527, 706, 1256
Cac8I GCNNGC 1 cut(s) 111
CfoI GCGC 1 cut(s) 895
Cfr13I GGNCC 1 cut(s) 1513
CseI GACGC 3 cut(s) 46, 233, 646
Csp6I GTAC 6 cut(s) 545, 562, 711, 1037, 1178, 1196
CspCI CAANNNNNGTGG 2 cut(s) 484, 519
CviQI GTAC 6 cut(s) 545, 562, 711, 1037, 1178, 1196
DpnI GATC 8 cut(s) 201, 351, 522, 843, 909, 1596, 1654, 1725
DpnII GATC 8 cut(s) 199, 349, 520, 841, 907, 1594, 1652, 1723
DraI TTTAAA 1 cut(s) 256
DrdI GACNNNNNNGTC 1 cut(s) 442
DseDI GACNNNNNNGTC 1 cut(s) 442
Eam1104I CTCTTC 2 cut(s) 786, 1743
EarI CTCTTC 2 cut(s) 786, 1743
EciI GGCGGA 1 cut(s) 97
Eco130I CCWWGG 2 cut(s) 366, 623
Eco24I GRGCYC 1 cut(s) 1476
Eco32I GATATC 1 cut(s) 588
Eco47I GGWCC 1 cut(s) 1513
Eco57I CTGAAG 1 cut(s) 853
Eco81I CCTNAGG 1 cut(s) 1517
Eco88I CYCGRG 1 cut(s) 167
EcoRII CCWGG 1 cut(s) 540
EcoRV GATATC 1 cut(s) 588
EcoT14I CCWWGG 2 cut(s) 366, 623
EcoT38I GRGCYC 1 cut(s) 1476
ErhI CCWWGG 2 cut(s) 366, 623
Esp3I CGTCTC 1 cut(s) 428
FalI AAGNNNNNCTT 4 cut(s) 1642, 1674, 1740, 1772
FaqI GGGAC 1 cut(s) 348
FauNDI CATATG 1 cut(s) 615
FbaI TGATCA 1 cut(s) 520
FblI GTMKAC 2 cut(s) 292, 854
Fnu4HI GCNGC 9 cut(s) 71, 74, 77, 80, 145, 603, 1326, 1638, 1788
FokI GGATG 8 cut(s) 340, 944, 1117, 1136, 1334, 1402, 1540, 1556
FriOI GRGCYC 1 cut(s) 1476
Fsp4HI GCNGC 9 cut(s) 71, 74, 77, 80, 145, 603, 1326, 1638, 1788
FspBI CTAG 3 cut(s) 89, 624, 644
GlaI GCGC 1 cut(s) 894
GluI GCNGC 9 cut(s) 71, 74, 77, 80, 145, 603, 1326, 1638, 1788
GsaI CCCAGC 1 cut(s) 1496
GsuI CTGGAG 4 cut(s) 524, 542, 795, 1373
HgaI GACGC 3 cut(s) 46, 233, 646
HhaI GCGC 1 cut(s) 895
Hin6I GCGC 1 cut(s) 893
HinP1I GCGC 1 cut(s) 893
HincII GTYRAC 4 cut(s) 223, 630, 681, 1129
HindII GTYRAC 4 cut(s) 223, 630, 681, 1129
HindIII AAGCTT 2 cut(s) 761, 1345
HinfI GANTC 8 cut(s) 85, 116, 294, 703, 859, 935, 1220, 1244
HpaI GTTAAC 1 cut(s) 681
HphI GGTGA 2 cut(s) 1562, 1602
Hpy166II GTNNAC 6 cut(s) 223, 293, 630, 681, 855, 1129
Hpy188I TCNGA 6 cut(s) 204, 283, 433, 592, 1225, 1461
Hpy8I GTNNAC 6 cut(s) 223, 293, 630, 681, 855, 1129
Hpy99I CGWCG 2 cut(s) 62, 132
HpyAV CCTTC 6 cut(s) 111, 725, 1114, 1122, 1653, 1675
HpyCH4III ACNGT 5 cut(s) 185, 710, 769, 1260, 1540
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 8 cut(s) 239, 953, 1010, 1325, 1443, 1640, 1787, 1818
HpyF10VI GCNNNNNNNGC 4 cut(s) 54, 76, 79, 314
HpySE526I ACGT 1 cut(s) 219
HspAI GCGC 1 cut(s) 893
Ksp22I TGATCA 1 cut(s) 520
KspAI GTTAAC 1 cut(s) 681
Kzo9I GATC 8 cut(s) 199, 349, 520, 841, 907, 1594, 1652, 1723
LguI GCTCTTC 1 cut(s) 1743
LmnI GCTCC 7 cut(s) 19, 248, 343, 754, 776, 1171, 1795
Lsp1109I GCAGC 5 cut(s) 88, 589, 1337, 1624, 1799
LweI GCATC 4 cut(s) 1098, 1291, 1312, 1534
MaeI CTAG 3 cut(s) 89, 624, 644
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 4 cut(s) 403, 514, 658, 704
MalI GATC 8 cut(s) 201, 351, 522, 843, 909, 1596, 1654, 1725
MboI GATC 8 cut(s) 199, 349, 520, 841, 907, 1594, 1652, 1723
MboII GAAGA 6 cut(s) 145, 803, 1638, 1675, 1690, 1760
MfeI CAATTG 1 cut(s) 1826
MflI RGATCY 3 cut(s) 349, 841, 907
MhlI GDGCHC 2 cut(s) 96, 1476
MluCI AATT 7 cut(s) 718, 802, 967, 1021, 1297, 1465, 1826
MlyI GAGTC 5 cut(s) 94, 110, 288, 712, 1229
MmeI TCCRAC 2 cut(s) 997, 1689
MroXI GAANNNNTTC 2 cut(s) 1698, 1752
MslI CAYNNNNRTG 3 cut(s) 888, 1103, 1209
MspA1I CMGCKG 1 cut(s) 79
MspR9I CCNGG 1 cut(s) 542
MunI CAATTG 1 cut(s) 1826
Mva1269I GAATGC 1 cut(s) 695
MvaI CCWGG 1 cut(s) 542
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 4 cut(s) 54, 76, 79, 314
NdeI CATATG 1 cut(s) 615
NdeII GATC 8 cut(s) 199, 349, 520, 841, 907, 1594, 1652, 1723
NmuCI GTSAC 4 cut(s) 403, 514, 658, 704
NspI RCATGY 4 cut(s) 957, 1037, 1178, 1196
PaeR7I CTCGAG 1 cut(s) 167
PciSI GCTCTTC 1 cut(s) 1743
PctI GAATGC 1 cut(s) 695
PdmI GAANNNNTTC 2 cut(s) 1698, 1752
PfeI GAWTC 3 cut(s) 859, 935, 1244
PflFI GACNNNGTC 1 cut(s) 56
PflMI CCANNNNNTGG 1 cut(s) 16
PkrI GCNGC 9 cut(s) 72, 75, 78, 81, 146, 604, 1327, 1639, 1789
PleI GAGTC 5 cut(s) 93, 110, 288, 711, 1228
PpsI GAGTC 5 cut(s) 93, 110, 288, 711, 1228
PshAI GACNNNNGTC 2 cut(s) 656, 1436
PshBI ATTAAT 1 cut(s) 1446
Psp1406I AACGTT 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 540
PspFI CCCAGC 1 cut(s) 1492
PspGI CCWGG 1 cut(s) 540
PspPI GGNCC 1 cut(s) 1513
PspXI VCTCGAGB 1 cut(s) 167
PstI CTGCAG 1 cut(s) 1012
PsuI RGATCY 3 cut(s) 349, 841, 907
PsyI GACNNNGTC 1 cut(s) 56
RsaI GTAC 6 cut(s) 546, 563, 712, 1038, 1179, 1197
RsaNI GTAC 6 cut(s) 545, 562, 711, 1037, 1178, 1196
RseI CAYNNNNRTG 3 cut(s) 888, 1103, 1209
SapI GCTCTTC 1 cut(s) 1743
SatI GCNGC 9 cut(s) 71, 74, 77, 80, 145, 603, 1326, 1638, 1788
Sau3AI GATC 8 cut(s) 199, 349, 520, 841, 907, 1594, 1652, 1723
Sau96I GGNCC 1 cut(s) 1513
SchI GAGTC 5 cut(s) 94, 110, 288, 712, 1229
ScrFI CCNGG 1 cut(s) 542
SduI GDGCHC 2 cut(s) 96, 1476
SfaNI GCATC 4 cut(s) 1098, 1291, 1312, 1534
SfcI CTRYAG 1 cut(s) 1008
Sfr274I CTCGAG 1 cut(s) 167
SinI GGWCC 1 cut(s) 1513
SlaI CTCGAG 1 cut(s) 167
SmiMI CAYNNNNRTG 3 cut(s) 888, 1103, 1209
SmlI CTYRAG 5 cut(s) 167, 1046, 1132, 1217, 1235
SmoI CTYRAG 5 cut(s) 167, 1046, 1132, 1217, 1235
Sse9I AATT 7 cut(s) 718, 802, 967, 1021, 1297, 1465, 1826
SspMI CTAG 3 cut(s) 89, 624, 644
StyD4I CCNGG 1 cut(s) 540
StyI CCWWGG 2 cut(s) 366, 623
TaaI ACNGT 5 cut(s) 185, 710, 769, 1260, 1540
TaiI ACGT 1 cut(s) 222
TaqI TCGA 3 cut(s) 60, 168, 356
TasI AATT 7 cut(s) 718, 802, 967, 1021, 1297, 1465, 1826
TatI WGTACW 1 cut(s) 710
TauI GCSGC 4 cut(s) 73, 76, 82, 147
TfiI GAWTC 3 cut(s) 859, 935, 1244
TscAI CASTG 5 cut(s) 190, 523, 534, 713, 1263
TseFI GTSAC 4 cut(s) 403, 514, 658, 704
TseI GCWGC 5 cut(s) 76, 602, 1325, 1637, 1787
Tsp45I GTSAC 4 cut(s) 403, 514, 658, 704
TspDTI ATGAA 3 cut(s) 602, 1470, 1707
TspGWI ACGGA 1 cut(s) 116
TspRI CASTG 5 cut(s) 190, 523, 534, 713, 1263
Tth111I GACNNNGTC 1 cut(s) 56
Van91I CCANNNNNTGG 1 cut(s) 16
VpaK11BI GGWCC 1 cut(s) 1513
VspI ATTAAT 1 cut(s) 1446
XapI RAATTY 5 cut(s) 718, 967, 1021, 1297, 1465
XbaI TCTAGA 1 cut(s) 88
XceI RCATGY 4 cut(s) 957, 1037, 1178, 1196
XhoI CTCGAG 1 cut(s) 167
XmaJI CCTAGG 1 cut(s) 623
XmiI GTMKAC 2 cut(s) 292, 854
XmnI GAANNNNTTC 2 cut(s) 1698, 1752
XspI CTAG 3 cut(s) 89, 624, 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.