Rh7DG148200

Leucine-rich repeats, outliers

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0010274
Physical Location & Seq
Reverse (-)
12750184 .. 12754385
4202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG148200.1

Sequence Viewer

Length: 1848 bp
ATGGAGACCCAACTGGTGGAGCTCTGCTTAAAAGCGGCGTGTGAAAGCAGAGACAGCGTCGAGAAATGGCGGAGGCAGCGGCGGAGTCTGGAGCACTTGCCTTCTCAGCTCGCCGACGCTCTCCTCCGTCGCCTCATCTCTCGTCGCCTTCTCTTCCCTTCGTTGCTCGAGGTGTTCAAACACAGTGCAGAGGAGATTGATCTGAGAGGCGACAACAACGTTGACGCCGAATGGTTTGCATACATTGGAGCATTTCGTCATCTCCGAAGTTTGAATGTCTCTGGCTGCCGTAAACTCACAAGCTCAGCTCTTTGGGCTGTAGCAGGGATGAGGAGTTTAAGGGAGATGGATCTTTCGAGATGCTCCAAGGTTAATGATGCTGGCATTAGGCATCTTTCATCTGTTACAACTTTGGAAAAGTTATGGATTTCGGAGACGGGGGTGTCTGCTAAAGGGGTTATGCTTCTCTCTTCGCTTGGAAACTTGTCTGTTCTCGACTTGGGTGGTTTGCCTGTCACTGATCAAGCACTCAGTTCTCTCCAGGTACTTACTAAACTCCAGTATCTTGACCTTTGGGGGAGTAACATATCTGACAAGGGTGCTGCTCTTCTTCATATGTTCCCTAGGTTGAGTTTCCTCAATCTAGCTTGGACCAGCGTCACAAAGTTGCCAAATTGGTTAACCCTTGAATGCTTAAACATGAGTAACTGTACAATAAATCTACTTGAAGGACATAATGGTAAAGCTCCTTTAGTCAAGCTTATTGTTTCTGGAGCTACATTTGGAGAAGAGGTTGCTGATTTCCATTATATAGAAACAACCCTTTTAACCTTTTTGGATCTTTCCAAGTCTACAATTCTAGACTTCAGCTTCTTATCTCATCTAAATGCTCTGGAACACTTGGATCTCAGCTCTACTGTGATAGGAGATGATTCGCTTCAACTTATTGCATGTGTTGGAGTGAATTTGAAATATCTGAATCTTAACAACACCAAAGTAAGCTCTGCAGGAGTTGGAAATTTAACAGGGCGTGTACCTAATCTTCAGGTTTTGTCTATATCACATACATCAGTAGATGATGTTACCATATCTTACATTGGCATGATGCCTTCTCTGAAGGTTATTGACCTGAGCAATACCAACATTAAAGGTGTCATTTACCAGGCAGAGACTGTGCCTGTGCGTGAGCCTGAGCCTGAGCCTGAGCATGAGCATGAGCCTGAGCATGAGCCAGAGCCTGAGCATGAGCCTGAGTCAGTTTCAGTCCTCTCAATGTCAGCACTTCAAAGTCTCAGGCATCTGGAAAGGTTGAATTTGGCAGATACACAAGTTATGGATGCAGCTCTATACCCTTTGACAAGCTTCCCAAAGTTAAGCCATTTATCTCTTAAAAGTGCTTCTCTTACAGACATCTCCCTTAACTACGTGTCATCCATATCAAAGCTGACAAATGTCTGCATTCATGATGGTGTTCTGACAAACTCTGGGCTCAATTCATTCAAACCCCCAGCAACTCTTAGAATGATGGACCTGAGGGGTTGTTGGCTCTTAACTGAGGATGCTATCACATCCTTCTCTAAAACTCACCCCCAGATTGAAGTATGGCATGATCTTGTTCACATCTCACGATCAAACCAGACTGTTTGTAATACTGCACCTTCTTCAAGATCAACTTTGAGGACTTCACAAGTGAAGAAGAAGCATGAAAGTATTCCCATGTTGGAATGTTTTTTAGATCAAAGATTGAAGTATAGCAGAGAAGAGCTCCTTTCCATGCGGTTCTCGTCTGTTTCCCTTGCAGCTTCTATTGATAGCGGCATTGGAATATGCAACACACAATCGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_6 LRR_8

Protein Analysis

615

Amino Acids

68.11

Weight (kDa)

5.97

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 82 - 241 2.2e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 442
AccB7I CCANNNNNTGG 1 cut(s) 16
AccI GTMKAC 1 cut(s) 851
AciI CCGC 6 cut(s) 35, 70, 79, 82, 1777, 1815
AclI AACGTT 1 cut(s) 219
AclWI GGATC 3 cut(s) 357, 846, 912
AcsI RAATTY 3 cut(s) 964, 1018, 1312
AcuI CTGAAG 3 cut(s) 850, 1028, 1136
AcyI GRCGYC 1 cut(s) 225
AfaI GTAC 3 cut(s) 546, 712, 1035
AfiI CCNNNNNNNGG 1 cut(s) 16
AflIII ACRYGT 1 cut(s) 1425
AjnI CCWGG 2 cut(s) 540, 1161
Alw21I GWGCWC 3 cut(s) 24, 96, 1767
Alw26I GTCTC 5 cut(s) 45, 283, 428, 1163, 1295
AlwI GGATC 3 cut(s) 357, 846, 912
AlwNI CAGNNNCTG 2 cut(s) 1172, 1238
Ama87I CYCGRG 1 cut(s) 167
ApeKI GCWGC 5 cut(s) 76, 285, 602, 1340, 1799
ApoI RAATTY 3 cut(s) 964, 1018, 1312
Asp700I GAANNNNTTC 1 cut(s) 1710
AspA2I CCTAGG 1 cut(s) 623
AspS9I GGNCC 2 cut(s) 651, 1528
AsuHPI GGTGA 1 cut(s) 1577
AvaI CYCGRG 1 cut(s) 167
AvaII GGWCC 2 cut(s) 651, 1528
AvrII CCTAGG 1 cut(s) 623
AxyI CCTNAGG 1 cut(s) 1532
BaeI ACNNNNGTAYC 4 cut(s) 545, 578, 1314, 1347
BanII GRGCYC 3 cut(s) 24, 1491, 1767
BarI GAAGNNNNNNTAC 2 cut(s) 1026, 1058
Bbv12I GWGCWC 3 cut(s) 24, 96, 1767
BbvI GCAGC 5 cut(s) 88, 272, 589, 1352, 1811
BccI CCATC 3 cut(s) 340, 1460, 1519
BceAI ACGGC 1 cut(s) 273
BcgI CGANNNNNNTGC 2 cut(s) 218, 252
BciT130I CCWGG 2 cut(s) 542, 1163
BclI TGATCA 1 cut(s) 520
BcoDI GTCTC 5 cut(s) 45, 283, 428, 1163, 1295
BfaI CTAG 3 cut(s) 624, 644, 860
BfmI CTRYAG 2 cut(s) 318, 1005
BisI GCNGC 8 cut(s) 36, 77, 80, 286, 603, 1341, 1800, 1816
BlnI CCTAGG 1 cut(s) 623
BlpI GCTNAGC 1 cut(s) 304
BlsI GCNGC 8 cut(s) 37, 78, 81, 287, 604, 1342, 1801, 1817
Bme1390I CCNGG 2 cut(s) 542, 1163
Bme18I GGWCC 2 cut(s) 651, 1528
BmeT110I CYCGRG 1 cut(s) 167
BmgT120I GGNCC 2 cut(s) 651, 1528
BmrFI CCNGG 2 cut(s) 542, 1163
BmsI GCATC 7 cut(s) 350, 367, 400, 1095, 1306, 1327, 1549
BoxI GACNNNNGTC 2 cut(s) 656, 1451
BplI GAGNNNNNCTC 2 cut(s) 1749, 1781
BpmI CTGGAG 4 cut(s) 110, 524, 542, 792
Bpu10I CCTNAGC 6 cut(s) 1130, 1191, 1197, 1203, 1221, 1239
Bpu1102I GCTNAGC 1 cut(s) 304
BsaAI YACGTR 1 cut(s) 1426
BsaHI GRCGYC 1 cut(s) 225
BsaJI CCNNGG 2 cut(s) 366, 623
BsaXI ACNNNNNCTCC 2 cut(s) 777, 807
Bsc4I CCNNNNNNNGG 1 cut(s) 16
Bse1I ACTGG 2 cut(s) 18, 559
Bse21I CCTNAGG 1 cut(s) 1532
BseBI CCWGG 2 cut(s) 542, 1163
BseDI CCNNGG 2 cut(s) 366, 623
BseGI GGATG 5 cut(s) 333, 1342, 1430, 1564, 1568
BseLI CCNNNNNNNGG 1 cut(s) 16
BseNI ACTGG 2 cut(s) 18, 559
BseRI GAGGAG 3 cut(s) 113, 206, 346
BseXI GCAGC 5 cut(s) 88, 272, 589, 1352, 1811
BseYI CCCAGC 1 cut(s) 1507
BsgI GTGCAG 2 cut(s) 207, 1638
BsiHKAI GWGCWC 3 cut(s) 24, 96, 1767
BsiHKCI CYCGRG 1 cut(s) 167
BslI CCNNNNNNNGG 1 cut(s) 16
BsmAI GTCTC 5 cut(s) 45, 283, 428, 1163, 1295
BsmBI CGTCTC 1 cut(s) 428
BsmI GAATGC 2 cut(s) 695, 1458
BsoBI CYCGRG 1 cut(s) 167
Bsp1286I GDGCHC 4 cut(s) 24, 96, 1491, 1767
Bsp1407I TGTACA 1 cut(s) 710
Bsp143I GATC 9 cut(s) 199, 349, 520, 838, 904, 1609, 1628, 1667, 1735
Bsp1720I GCTNAGC 1 cut(s) 304
BspACI CCGC 6 cut(s) 35, 70, 79, 82, 1777, 1815
BspHI TCATGA 1 cut(s) 1462
BspMAI CTGCAG 1 cut(s) 1009
BspPI GGATC 3 cut(s) 357, 846, 912
BspQI GCTCTTC 2 cut(s) 612, 1755
BsrGI TGTACA 1 cut(s) 710
BsrI ACTGG 2 cut(s) 18, 559
BssECI CCNNGG 2 cut(s) 366, 623
BssMI GATC 9 cut(s) 199, 349, 520, 838, 904, 1609, 1628, 1667, 1735
BssNI GRCGYC 1 cut(s) 225
BssT1I CCWWGG 2 cut(s) 366, 623
Bst2UI CCWGG 2 cut(s) 542, 1163
Bst4CI ACNGT 5 cut(s) 185, 710, 919, 1174, 1642
Bst6I CTCTTC 5 cut(s) 158, 475, 612, 783, 1755
BstACI GRCGYC 1 cut(s) 225
BstAUI TGTACA 1 cut(s) 710
BstBAI YACGTR 1 cut(s) 1426
BstC8I GCNNGC 2 cut(s) 111, 382
BstF5I GGATG 5 cut(s) 333, 1342, 1430, 1564, 1568
BstKTI GATC 9 cut(s) 202, 352, 523, 841, 907, 1612, 1631, 1670, 1738
BstMAI GTCTC 5 cut(s) 45, 283, 428, 1163, 1295
BstMBI GATC 9 cut(s) 199, 349, 520, 838, 904, 1609, 1628, 1667, 1735
BstMWI GCNNNNNNNGC 4 cut(s) 54, 76, 106, 314
BstNI CCWGG 2 cut(s) 542, 1163
BstNSI RCATGY 1 cut(s) 954
BstPAI GACNNNNGTC 2 cut(s) 656, 1451
BstSCI CCNGG 2 cut(s) 540, 1161
BstSFI CTRYAG 2 cut(s) 318, 1005
BstV1I GCAGC 5 cut(s) 88, 272, 589, 1352, 1811
BstX2I RGATCY 3 cut(s) 349, 838, 904
BstYI RGATCY 3 cut(s) 349, 838, 904
Bsu36I CCTNAGG 1 cut(s) 1532
BtsCI GGATG 5 cut(s) 333, 1342, 1430, 1564, 1568
BtsIMutI CAGTG 2 cut(s) 190, 516
Cac8I GCNNGC 2 cut(s) 111, 382
CaiI CAGNNNCTG 2 cut(s) 1172, 1238
CciI TCATGA 1 cut(s) 1462
Cfr13I GGNCC 2 cut(s) 651, 1528
CseI GACGC 4 cut(s) 46, 125, 233, 646
Csp6I GTAC 3 cut(s) 545, 711, 1034
CviQI GTAC 3 cut(s) 545, 711, 1034
DpnI GATC 9 cut(s) 201, 351, 522, 840, 906, 1611, 1630, 1669, 1737
DpnII GATC 9 cut(s) 199, 349, 520, 838, 904, 1609, 1628, 1667, 1735
DrdI GACNNNNNNGTC 1 cut(s) 442
DseDI GACNNNNNNGTC 1 cut(s) 442
Eam1104I CTCTTC 5 cut(s) 158, 475, 612, 783, 1755
EarI CTCTTC 5 cut(s) 158, 475, 612, 783, 1755
EciI GGCGGA 2 cut(s) 85, 97
Ecl136II GAGCTC 2 cut(s) 22, 1765
Eco130I CCWWGG 2 cut(s) 366, 623
Eco24I GRGCYC 3 cut(s) 24, 1491, 1767
Eco47I GGWCC 2 cut(s) 651, 1528
Eco53kI GAGCTC 2 cut(s) 22, 1765
Eco57I CTGAAG 3 cut(s) 850, 1028, 1136
Eco81I CCTNAGG 1 cut(s) 1532
Eco88I CYCGRG 1 cut(s) 167
EcoICRI GAGCTC 2 cut(s) 22, 1765
EcoRII CCWGG 2 cut(s) 540, 1161
EcoT14I CCWWGG 2 cut(s) 366, 623
EcoT38I GRGCYC 3 cut(s) 24, 1491, 1767
ErhI CCWWGG 2 cut(s) 366, 623
Esp3I CGTCTC 1 cut(s) 428
FalI AAGNNNNNCTT 4 cut(s) 1657, 1689, 1752, 1784
FauNDI CATATG 1 cut(s) 615
FbaI TGATCA 1 cut(s) 520
FblI GTMKAC 1 cut(s) 851
Fnu4HI GCNGC 8 cut(s) 36, 77, 80, 286, 603, 1341, 1800, 1816
FokI GGATG 5 cut(s) 340, 1349, 1417, 1555, 1571
FriOI GRGCYC 3 cut(s) 24, 1491, 1767
Fsp4HI GCNGC 8 cut(s) 36, 77, 80, 286, 603, 1341, 1800, 1816
FspBI CTAG 3 cut(s) 624, 644, 860
GluI GCNGC 8 cut(s) 36, 77, 80, 286, 603, 1341, 1800, 1816
GsaI CCCAGC 1 cut(s) 1511
GsuI CTGGAG 4 cut(s) 110, 524, 542, 792
HgaI GACGC 4 cut(s) 46, 125, 233, 646
Hin1I GRCGYC 1 cut(s) 225
HincII GTYRAC 2 cut(s) 223, 681
HindII GTYRAC 2 cut(s) 223, 681
HindIII AAGCTT 2 cut(s) 758, 1360
HinfI GANTC 4 cut(s) 85, 932, 979, 1253
HpaI GTTAAC 1 cut(s) 681
HphI GGTGA 1 cut(s) 1577
Hpy166II GTNNAC 6 cut(s) 223, 293, 681, 852, 1034, 1618
Hpy188I TCNGA 8 cut(s) 204, 266, 433, 592, 978, 1116, 1476, 1843
Hpy8I GTNNAC 6 cut(s) 223, 293, 681, 852, 1034, 1618
Hpy99I CGWCG 4 cut(s) 62, 119, 132, 147
HpyAV CCTTC 8 cut(s) 111, 158, 168, 722, 1111, 1119, 1582, 1668
HpyCH4III ACNGT 5 cut(s) 185, 710, 919, 1174, 1642
HpyCH4IV ACGT 2 cut(s) 219, 1425
HpyCH4V TGCA 9 cut(s) 188, 239, 950, 1007, 1340, 1458, 1655, 1799, 1830
HpyF10VI GCNNNNNNNGC 4 cut(s) 54, 76, 106, 314
HpySE526I ACGT 2 cut(s) 219, 1425
Hsp92I GRCGYC 1 cut(s) 225
Ksp22I TGATCA 1 cut(s) 520
KspAI GTTAAC 1 cut(s) 681
Kzo9I GATC 9 cut(s) 199, 349, 520, 838, 904, 1609, 1628, 1667, 1735
LguI GCTCTTC 2 cut(s) 612, 1755
LmnI GCTCC 7 cut(s) 19, 91, 248, 368, 751, 773, 1770
Lsp1109I GCAGC 5 cut(s) 88, 272, 589, 1352, 1811
LweI GCATC 7 cut(s) 350, 367, 400, 1095, 1306, 1327, 1549
MaeI CTAG 3 cut(s) 624, 644, 860
MaeII ACGT 2 cut(s) 219, 1425
MaeIII GTNAC 6 cut(s) 403, 514, 581, 658, 704, 1081
MalI GATC 9 cut(s) 201, 351, 522, 840, 906, 1611, 1630, 1669, 1737
MboI GATC 9 cut(s) 199, 349, 520, 838, 904, 1609, 1628, 1667, 1735
MflI RGATCY 3 cut(s) 349, 838, 904
MhlI GDGCHC 4 cut(s) 24, 96, 1491, 1767
MluCI AATT 6 cut(s) 673, 855, 964, 1018, 1312, 1492
MlyI GAGTC 2 cut(s) 94, 1262
MmeI TCCRAC 3 cut(s) 937, 994, 1701
MroXI GAANNNNTTC 1 cut(s) 1710
MslI CAYNNNNRTG 4 cut(s) 885, 1100, 1212, 1467
MspA1I CMGCKG 1 cut(s) 79
MspR9I CCNGG 2 cut(s) 542, 1163
Mva1269I GAATGC 2 cut(s) 695, 1458
MvaI CCWGG 2 cut(s) 542, 1163
MwoI GCNNNNNNNGC 4 cut(s) 54, 76, 106, 314
NdeI CATATG 1 cut(s) 615
NdeII GATC 9 cut(s) 199, 349, 520, 838, 904, 1609, 1628, 1667, 1735
NmuCI GTSAC 2 cut(s) 514, 658
NspI RCATGY 1 cut(s) 954
PaeR7I CTCGAG 1 cut(s) 167
PagI TCATGA 1 cut(s) 1462
PciSI GCTCTTC 2 cut(s) 612, 1755
PcsI WCGNNNNNNNCGW 2 cut(s) 225, 262
PctI GAATGC 2 cut(s) 695, 1458
PdmI GAANNNNTTC 1 cut(s) 1710
PfeI GAWTC 2 cut(s) 932, 979
PflFI GACNNNGTC 1 cut(s) 56
PflMI CCANNNNNTGG 1 cut(s) 16
PkrI GCNGC 8 cut(s) 37, 78, 81, 287, 604, 1342, 1801, 1817
PleI GAGTC 2 cut(s) 93, 1261
PpsI GAGTC 2 cut(s) 93, 1261
Ppu21I YACGTR 1 cut(s) 1426
PshAI GACNNNNGTC 2 cut(s) 656, 1451
Psp124BI GAGCTC 2 cut(s) 24, 1767
Psp1406I AACGTT 1 cut(s) 219
Psp6I CCWGG 2 cut(s) 540, 1161
PspFI CCCAGC 1 cut(s) 1507
PspGI CCWGG 2 cut(s) 540, 1161
PspPI GGNCC 2 cut(s) 651, 1528
PspXI VCTCGAGB 1 cut(s) 167
PstI CTGCAG 1 cut(s) 1009
PstNI CAGNNNCTG 2 cut(s) 1172, 1238
PsuI RGATCY 3 cut(s) 349, 838, 904
PsyI GACNNNGTC 1 cut(s) 56
RsaI GTAC 3 cut(s) 546, 712, 1035
RsaNI GTAC 3 cut(s) 545, 711, 1034
RseI CAYNNNNRTG 4 cut(s) 885, 1100, 1212, 1467
SacI GAGCTC 2 cut(s) 24, 1767
SapI GCTCTTC 2 cut(s) 612, 1755
SatI GCNGC 8 cut(s) 36, 77, 80, 286, 603, 1341, 1800, 1816
Sau3AI GATC 9 cut(s) 199, 349, 520, 838, 904, 1609, 1628, 1667, 1735
Sau96I GGNCC 2 cut(s) 651, 1528
SchI GAGTC 2 cut(s) 94, 1262
ScrFI CCNGG 2 cut(s) 542, 1163
SduI GDGCHC 4 cut(s) 24, 96, 1491, 1767
SfaNI GCATC 7 cut(s) 350, 367, 400, 1095, 1306, 1327, 1549
SfcI CTRYAG 2 cut(s) 318, 1005
Sfr274I CTCGAG 1 cut(s) 167
SinI GGWCC 2 cut(s) 651, 1528
SlaI CTCGAG 1 cut(s) 167
SmiMI CAYNNNNRTG 4 cut(s) 885, 1100, 1212, 1467
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
Sse9I AATT 6 cut(s) 673, 855, 964, 1018, 1312, 1492
SsiI CCGC 6 cut(s) 35, 70, 79, 82, 1777, 1815
SspMI CTAG 3 cut(s) 624, 644, 860
SstI GAGCTC 2 cut(s) 24, 1767
StyD4I CCNGG 2 cut(s) 540, 1161
StyI CCWWGG 2 cut(s) 366, 623
TaaI ACNGT 5 cut(s) 185, 710, 919, 1174, 1642
TaiI ACGT 2 cut(s) 222, 1428
TaqI TCGA 4 cut(s) 60, 168, 356, 495
TasI AATT 6 cut(s) 673, 855, 964, 1018, 1312, 1492
TatI WGTACW 1 cut(s) 710
TauI GCSGC 3 cut(s) 38, 82, 1818
TfiI GAWTC 2 cut(s) 932, 979
TscAI CASTG 2 cut(s) 190, 523
TseFI GTSAC 2 cut(s) 514, 658
TseI GCWGC 5 cut(s) 76, 285, 602, 1340, 1799
Tsp45I GTSAC 2 cut(s) 514, 658
TspDTI ATGAA 5 cut(s) 387, 602, 1451, 1485, 1719
TspGWI ACGGA 1 cut(s) 116
TspRI CASTG 2 cut(s) 190, 523
Tth111I GACNNNGTC 1 cut(s) 56
Van91I CCANNNNNTGG 1 cut(s) 16
VpaK11BI GGWCC 2 cut(s) 651, 1528
XapI RAATTY 3 cut(s) 964, 1018, 1312
XbaI TCTAGA 1 cut(s) 859
XceI RCATGY 1 cut(s) 954
XhoI CTCGAG 1 cut(s) 167
XmaJI CCTAGG 1 cut(s) 623
XmiI GTMKAC 1 cut(s) 851
XmnI GAANNNNTTC 1 cut(s) 1710
XspI CTAG 3 cut(s) 624, 644, 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.