Rorug07G0019300

Leucine-rich repeats, outliers

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0010274
Physical Location & Seq
Reverse (-)
1468720 .. 1469976
1257 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0019300.1

Sequence Viewer

Length: 675 bp
ATGGGAGGGGTGAAGCTGGTTGCTACAACTCAAAGCTTTTTCTGCACTAGGGTTCAGTGGGCTCTGAAGCTCAAAGGCGTGGAGTATGAGTTTATAGAAGAAGATTTAAGAAACAAGAGCCCCATCCTGCTCAAGTATAACCCTGTCTACAAGAAGGTCCCTGTGCTTGTGCACCATGACACACCCATTGCCGAGTCACTTGTGATCCTTGAGTACATCGATGAGACATGGAAGGAGAACCCCTTACTTCCTCAGGACCCTTGTGACAGAGCCGCAGCTCGCTTTTGGGCTAAGTTTGCTGATGAGAAGGTTGTGTTTGGAGTATGGACAGCTTGCACGATGGAAGGAGAAGGGAAGGAGAAGGCAATAGAGGCTGCTCTAGAATCACTAGCTCATCTTGAGAAGCAGATTGAAGGGAAGAAGTTTTTTGGTGGAGAACAAATAGGGTATCTGGATTTAGTACTGGGTTGGATACCTCATTGGCTCAATACTATGGAAGAAGTTGGAGGCATGAAGCTACTTGAAGCCGAGAAGTTCCCATGTCTCATTGAATGGGGTCAGAACTTCATCCATACTCCACTAATCAAAGAATGCATTCCACCCAGAGAGAAGCTTATCGAATACTTGACCGCTAGCCTCAGCTACCTGCGTTCCTTAGCAGCCAAAAAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_6 LRR_8

Protein Analysis

224

Amino Acids

25.58

Weight (kDa)

5.65

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 4 - 75 7.8e-19 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 8 - 79 2.6e-14 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 12 - 76 5.3e-15 Glutathione S-transferase, N-terminal domain
GST_C_2 PF13410 120 - 185 7.5e-06 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 654
AccI GTMKAC 1 cut(s) 147
AciI CCGC 2 cut(s) 273, 630
AclWI GGATC 1 cut(s) 199
AcuI CTGAAG 1 cut(s) 86
AfaI GTAC 2 cut(s) 215, 462
AgsI TTSAA 3 cut(s) 413, 524, 551
AluBI AGCT 9 cut(s) 16, 36, 70, 278, 332, 392, 517, 613, 642
AluI AGCT 9 cut(s) 16, 36, 70, 278, 332, 392, 517, 613, 642
Alw21I GWGCWC 1 cut(s) 174
Alw26I GTCTC 2 cut(s) 218, 548
Alw44I GTGCAC 1 cut(s) 170
AlwI GGATC 1 cut(s) 199
ApaLI GTGCAC 1 cut(s) 170
ApeKI GCWGC 3 cut(s) 275, 374, 659
Asp700I GAANNNNTTC 1 cut(s) 594
AspS9I GGNCC 2 cut(s) 157, 256
AsuHPI GGTGA 1 cut(s) 22
AsuNHI GCTAGC 1 cut(s) 632
AvaII GGWCC 2 cut(s) 157, 256
AxyI CCTNAGG 1 cut(s) 252
BaeGI GKGCMC 1 cut(s) 174
BanII GRGCYC 2 cut(s) 64, 122
Bbv12I GWGCWC 1 cut(s) 174
BbvCI CCTCAGC 1 cut(s) 638
BbvI GCAGC 3 cut(s) 287, 361, 671
BccI CCATC 2 cut(s) 131, 334
BciVI GTATCC 1 cut(s) 465
BcoDI GTCTC 2 cut(s) 218, 548
BfaI CTAG 4 cut(s) 48, 380, 389, 633
BfuAI ACCTGC 1 cut(s) 654
BfuI GTATCC 1 cut(s) 465
BisI GCNGC 4 cut(s) 273, 276, 375, 660
BlsI GCNGC 4 cut(s) 274, 277, 376, 661
BmcAI AGTACT 1 cut(s) 462
Bme18I GGWCC 2 cut(s) 157, 256
BmgT120I GGNCC 2 cut(s) 157, 256
BmiI GGNNCC 2 cut(s) 159, 258
BmrI ACTGGG 1 cut(s) 473
BmtI GCTAGC 1 cut(s) 636
BmuI ACTGGG 1 cut(s) 473
Bpu10I CCTNAGC 2 cut(s) 638, 655
BpuEI CTTGAG 3 cut(s) 116, 230, 419
Bsa29I ATCGAT 1 cut(s) 219
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bse1I ACTGG 1 cut(s) 468
Bse21I CCTNAGG 1 cut(s) 252
Bse3DI GCAATG 1 cut(s) 186
BseCI ATCGAT 1 cut(s) 219
BseGI GGATG 2 cut(s) 123, 567
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 2 cut(s) 266, 652
BseNI ACTGG 1 cut(s) 468
BseSI GKGCMC 1 cut(s) 174
BseXI GCAGC 3 cut(s) 287, 361, 671
BsgI GTGCAG 1 cut(s) 28
BshVI ATCGAT 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 174
BslFI GGGAC 1 cut(s) 143
BsmAI GTCTC 2 cut(s) 218, 548
BsmFI GGGAC 1 cut(s) 143
BsmI GAATGC 2 cut(s) 594, 596
Bsp1286I GDGCHC 3 cut(s) 64, 122, 174
Bsp143I GATC 1 cut(s) 204
BspACI CCGC 2 cut(s) 273, 630
BspCNI CTCAG 2 cut(s) 265, 651
BspDI ATCGAT 1 cut(s) 219
BspLI GGNNCC 2 cut(s) 159, 258
BspMI ACCTGC 1 cut(s) 654
BspOI GCTAGC 1 cut(s) 636
BspPI GGATC 1 cut(s) 199
BsrDI GCAATG 1 cut(s) 186
BsrI ACTGG 1 cut(s) 468
BssMI GATC 1 cut(s) 204
BstC8I GCNNGC 3 cut(s) 280, 334, 634
BstDEI CTNAG 4 cut(s) 252, 291, 638, 655
BstF5I GGATG 2 cut(s) 123, 567
BstKTI GATC 1 cut(s) 207
BstMAI GTCTC 2 cut(s) 218, 548
BstMBI GATC 1 cut(s) 204
BstMWI GCNNNNNNNGC 3 cut(s) 42, 296, 371
BstSLI GKGCMC 1 cut(s) 174
BstV1I GCAGC 3 cut(s) 287, 361, 671
Bsu15I ATCGAT 1 cut(s) 219
Bsu36I CCTNAGG 1 cut(s) 252
BsuI GTATCC 1 cut(s) 465
BsuTUI ATCGAT 1 cut(s) 219
BtsCI GGATG 2 cut(s) 123, 567
BtsIMutI CAGTG 1 cut(s) 62
BveI ACCTGC 1 cut(s) 654
Cac8I GCNNGC 3 cut(s) 280, 334, 634
Cfr13I GGNCC 2 cut(s) 157, 256
ClaI ATCGAT 1 cut(s) 219
Csp6I GTAC 2 cut(s) 214, 461
CviAII CATG 5 cut(s) 176, 228, 511, 540, 672
CviQI GTAC 2 cut(s) 214, 461
DdeI CTNAG 4 cut(s) 252, 291, 638, 655
DpnI GATC 1 cut(s) 206
DpnII GATC 1 cut(s) 204
Eco24I GRGCYC 2 cut(s) 64, 122
Eco47I GGWCC 2 cut(s) 157, 256
Eco57I CTGAAG 1 cut(s) 86
Eco81I CCTNAGG 1 cut(s) 252
EcoO109I RGGNCCY 2 cut(s) 157, 256
EcoT22I ATGCAT 1 cut(s) 596
EcoT38I GRGCYC 2 cut(s) 64, 122
FaeI CATG 5 cut(s) 179, 231, 514, 543, 675
FaqI GGGAC 1 cut(s) 143
FatI CATG 5 cut(s) 175, 227, 510, 539, 671
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 4 cut(s) 273, 276, 375, 660
FokI GGATG 2 cut(s) 110, 554
FriOI GRGCYC 2 cut(s) 64, 122
Fsp4HI GCNGC 4 cut(s) 273, 276, 375, 660
FspBI CTAG 4 cut(s) 48, 380, 389, 633
GluI GCNGC 4 cut(s) 273, 276, 375, 660
Hin1II CATG 5 cut(s) 179, 231, 514, 543, 675
HindIII AAGCTT 2 cut(s) 34, 611
HinfI GANTC 2 cut(s) 194, 383
HphI GGTGA 1 cut(s) 22
Hpy166II GTNNAC 2 cut(s) 148, 172
Hpy188I TCNGA 2 cut(s) 66, 561
Hpy188III TCNNGA 4 cut(s) 254, 380, 398, 452
Hpy8I GTNNAC 2 cut(s) 148, 172
HpyAV CCTTC 8 cut(s) 148, 226, 301, 338, 344, 349, 355, 407
HpyCH4V TGCA 4 cut(s) 45, 172, 336, 594
HpyF10VI GCNNNNNNNGC 3 cut(s) 42, 296, 371
HpyF3I CTNAG 4 cut(s) 252, 291, 638, 655
Hsp92II CATG 5 cut(s) 179, 231, 514, 543, 675
Kzo9I GATC 1 cut(s) 204
LpnPI CCDG 9 cut(s) 2, 140, 156, 174, 239, 437, 449, 616, 659
Lsp1109I GCAGC 3 cut(s) 287, 361, 671
MaeI CTAG 4 cut(s) 48, 380, 389, 633
MaeIII GTNAC 2 cut(s) 195, 263
MalI GATC 1 cut(s) 206
MboI GATC 1 cut(s) 204
MboII GAAGA 4 cut(s) 110, 113, 430, 509
MhlI GDGCHC 3 cut(s) 64, 122, 174
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 2 cut(s) 449, 484
MnlI CCTC 5 cut(s) 261, 364, 486, 500, 647
Mph1103I ATGCAT 1 cut(s) 596
MroXI GAANNNNTTC 1 cut(s) 594
MseI TTAA 1 cut(s) 107
Mva1269I GAATGC 2 cut(s) 594, 596
MwoI GCNNNNNNNGC 3 cut(s) 42, 296, 371
NdeII GATC 1 cut(s) 204
NheI GCTAGC 1 cut(s) 632
NlaIII CATG 5 cut(s) 179, 231, 514, 543, 675
NlaIV GGNNCC 2 cut(s) 159, 258
NmeAIII GCCGAG 2 cut(s) 217, 553
NmuCI GTSAC 2 cut(s) 195, 263
NsiI ATGCAT 1 cut(s) 596
PctI GAATGC 2 cut(s) 594, 596
PdmI GAANNNNTTC 1 cut(s) 594
PfeI GAWTC 1 cut(s) 383
PkrI GCNGC 4 cut(s) 274, 277, 376, 661
PleI GAGTC 1 cut(s) 202
PpsI GAGTC 1 cut(s) 202
PpuMI RGGWCCY 2 cut(s) 157, 256
Psp5II RGGWCCY 2 cut(s) 157, 256
PspN4I GGNNCC 2 cut(s) 159, 258
PspPI GGNCC 2 cut(s) 157, 256
PspPPI RGGWCCY 2 cut(s) 157, 256
RsaI GTAC 2 cut(s) 215, 462
RsaNI GTAC 2 cut(s) 214, 461
SaqAI TTAA 1 cut(s) 107
SatI GCNGC 4 cut(s) 273, 276, 375, 660
Sau3AI GATC 1 cut(s) 204
Sau96I GGNCC 2 cut(s) 157, 256
ScaI AGTACT 1 cut(s) 462
SchI GAGTC 1 cut(s) 203
SduI GDGCHC 3 cut(s) 64, 122, 174
SinI GGWCC 2 cut(s) 157, 256
SmlI CTYRAG 3 cut(s) 131, 209, 398
SmoI CTYRAG 3 cut(s) 131, 209, 398
SsiI CCGC 2 cut(s) 273, 630
SspMI CTAG 4 cut(s) 48, 380, 389, 633
TaqI TCGA 2 cut(s) 219, 618
TatI WGTACW 2 cut(s) 213, 460
TauI GCSGC 1 cut(s) 275
TfiI GAWTC 1 cut(s) 383
Tru1I TTAA 1 cut(s) 107
Tru9I TTAA 1 cut(s) 107
TscAI CASTG 1 cut(s) 62
TseFI GTSAC 2 cut(s) 195, 263
TseI GCWGC 3 cut(s) 275, 374, 659
Tsp45I GTSAC 2 cut(s) 195, 263
TspDTI ATGAA 2 cut(s) 527, 556
TspRI CASTG 1 cut(s) 62
VneI GTGCAC 1 cut(s) 170
VpaK11BI GGWCC 2 cut(s) 157, 256
XbaI TCTAGA 1 cut(s) 379
XmiI GTMKAC 1 cut(s) 147
XmnI GAANNNNTTC 1 cut(s) 594
XspI CTAG 4 cut(s) 48, 380, 389, 633
ZrmI AGTACT 1 cut(s) 462
Zsp2I ATGCAT 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.