FvH4_5g04720

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008591
Physical Location & Seq
Forward (+)
2762563 .. 2767223
4661 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g04720.t1

Sequence Viewer

Length: 1692 bp
ATGACTTTATACCTGGGCTCCCCACCATGGCTCTCTCGCCCATCTCCACCTCTCCCCACTTTCAACAACACCAACGCCTTGTTCTTCAGGCTAAGCTTCACTTCAGTCAACTCCCAAAAGCTCATTACAACAAAGGCGTCGTCTTTGGGCGAGAGTGAAAGTGCTAATGGGATTGCAGAGGACTCTGTTTCTGAGCTTTTGGACGAGGACTTGTTAAGTAAAGTTTCGGGTGCTAAAGATGCCCAAGAAGTACTGGAGATGTTTGATGAAATGGCTGAGAGAAAAAATGGGGGTGTGGTGAGTGTTTCTGATTGTTGTGATATTATCTCGGCTGCGCTGAAAAGGAACAACCCGGAATTGGCCCTCTCTGTTTTCTATGAAATGCGTGCCAGTTTTGATCAAGCTGTTGATGAAAATGGTCCGAGGTGGAGGTGGTCCAGACCAGATGTGAGTGTTTACACAACATTAATTCTGGGACTGGCTGCATCACTGAGAGTTTCTGATGCCCTTAAGATGATCAACAATATATGCCGAGTGGGAGTGTCTCCAGCTGAGGAGGTCCCCTTTGGAAAAGTTGTGAGATGTCCAAGCTGTATGATAGCTGTTGCTGTTGCACAACCACAACATGGTATTCAGATAGCATCGTGTGCAAAGTGCTCCTACCAATATGAACTTGTTTCTGGGAACATAGACAGTATTGCATCAGAAGAAATCGGCATGGATATTCCAGCTTGGAAAAGGGCACTAAGATTCCTGCAGATATTGAAGCAAAAGATTCCTGCTGCTGTTCACTCCATTGTGGTACAAACCCCTTCAGGTATGGCTCGTACACATAGATTTGCTACTGAAACAGTTGATCTCCCAGCACAGAAAGGAGAAAGGGTCACCATTGCTCTTGCAGCTCCATCAACTGTATATAGAGAGGTGGGTCCCTTAAGATTTAGTCCAAAGGCCCCTAATTTTTACCCTAGTGAGCCTATCTGCTTGACAAACCATGAGGATGGCCGGGAGTCACAACTGTTAAGAGCCCCTACAAAAGATGGTGGCACGTTGCTTACTAACCCCTCCATACTTTTTCCAATTGTTGCTGTTCTGGCCACTGGCAATGCTGCCTCTAGAGTTATAGACACCAGCCTCCCCCAACTCCTCTCCGTCACTGCAGTAACATCTCTTGCTGTTGGAGTTACTCTCAAAACATTGGTCTTGCCCCAATTGAATCGTCTTCCTCAGAGAGTAGTGGATACAGTTGCTATCAAACAGCAGCTATTATCTCAATACGACACACTCCAATCTCGTCTAAGGGGCCTAAAAGAAGCTGCTGAAAAAGAGGTCTGGATGTTGGCTCGGATGTGCCAGCTTGAGAACAAGATTTATGCTGTAGGAGAGCCATCTTATCGTGCTCGGAGAAGTAAAGTCAAAAGGGTTCGAGAAGGCTTGGAAAATTCTCTCAGGGGAAGAATTGAGCTGATTGACAGCTATGCGAAGATTTCTTCGATGATCGAGATTGAGGTAGAATTGGACTCTGATGTTCTTGCTGCTGAACAAGCAAGCAATGTGGAAAATATTGCGGAACAAATACAACAGACCATGGAACTAGAAAATCTTGAAGAGAGATGGAGAATACAGGTAGAAGCAAATGATGAGGCAGAAAGACTTCTTAGTTCCCAACCACTACCCACAGAACAATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

564

Amino Acids

61.98

Weight (kDa)

5.38

Isoelectric Point (pI)

52.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 626
AciI CCGC 1 cut(s) 1568
AcoI YGGCCR 2 cut(s) 1003, 1095
AcsI RAATTY 1 cut(s) 1441
AcuI CTGAAG 3 cut(s) 70, 87, 798
AcyI GRCGYC 1 cut(s) 137
AfaI GTAC 3 cut(s) 252, 804, 829
AfiI CCNNNNNNNGG 3 cut(s) 27, 358, 626
AflII CTTAAG 2 cut(s) 509, 934
AgsI TTSAA 4 cut(s) 64, 766, 1216, 1607
AjnI CCWGG 1 cut(s) 12
AjuI GAANNNNNNNTTGG 2 cut(s) 65, 97
Alw21I GWGCWC 2 cut(s) 659, 1402
Alw26I GTCTC 1 cut(s) 549
AoxI GGCC 5 cut(s) 360, 951, 1003, 1095, 1303
ApeKI GCWGC 8 cut(s) 332, 482, 782, 899, 1109, 1261, 1316, 1535
ApoI RAATTY 1 cut(s) 1441
ArsI GACNNNNNNTTYG 4 cut(s) 125, 157, 1185, 1217
AseI ATTAAT 1 cut(s) 467
Asp700I GAANNNNTTC 1 cut(s) 1653
AspLEI GCGC 1 cut(s) 337
AspS9I GGNCC 7 cut(s) 361, 419, 435, 559, 929, 952, 1303
AsuC2I CCSGG 2 cut(s) 353, 1007
AsuHPI GGTGA 2 cut(s) 310, 877
AvaII GGWCC 4 cut(s) 419, 435, 559, 929
BaeGI GKGCMC 1 cut(s) 745
BalI TGGCCA 1 cut(s) 1097
BanII GRGCYC 2 cut(s) 20, 1030
BbsI GAAGAC 1 cut(s) 1214
Bbv12I GWGCWC 2 cut(s) 659, 1402
BbvCI CCTCAGC 1 cut(s) 552
BbvI GCAGC 8 cut(s) 319, 469, 769, 911, 1096, 1273, 1303, 1522
BccI CCATC 6 cut(s) 49, 913, 995, 1034, 1396, 1608
BciT130I CCWGG 1 cut(s) 14
BciVI GTATCC 1 cut(s) 1234
BclI TGATCA 2 cut(s) 397, 516
BcnI CCSGG 2 cut(s) 353, 1007
BcoDI GTCTC 1 cut(s) 549
BfaI CTAG 3 cut(s) 969, 1116, 1595
BfmI CTRYAG 3 cut(s) 755, 1158, 1377
BfrI CTTAAG 2 cut(s) 509, 934
BfuI GTATCC 1 cut(s) 1234
BisI GCNGC 8 cut(s) 333, 483, 783, 900, 1110, 1262, 1317, 1536
BlpI GCTNAGC 1 cut(s) 92
BlsI GCNGC 8 cut(s) 334, 484, 784, 901, 1111, 1263, 1318, 1537
BmcAI AGTACT 1 cut(s) 252
Bme1390I CCNGG 3 cut(s) 14, 353, 1007
Bme18I GGWCC 4 cut(s) 419, 435, 559, 929
BmgT120I GGNCC 7 cut(s) 361, 419, 435, 559, 929, 952, 1303
BmiI GGNNCC 6 cut(s) 19, 561, 930, 931, 954, 1304
BmrFI CCNGG 3 cut(s) 14, 353, 1007
BmsI GCATC 5 cut(s) 229, 493, 494, 650, 710
BpiI GAAGAC 1 cut(s) 1214
BplI GAGNNNNNCTC 2 cut(s) 1173, 1205
BpmI CTGGAG 2 cut(s) 275, 531
Bpu10I CCTNAGC 1 cut(s) 552
Bpu1102I GCTNAGC 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 1379
BpuMI CCSGG 2 cut(s) 353, 1007
BsaHI GRCGYC 1 cut(s) 137
BsaJI CCNNGG 4 cut(s) 13, 26, 422, 1587
BsaXI ACNNNNNCTCC 3 cut(s) 32, 867, 897
Bsc4I CCNNNNNNNGG 3 cut(s) 27, 358, 626
Bse1I ACTGG 4 cut(s) 258, 390, 483, 1105
Bse3DI GCAATG 3 cut(s) 888, 1111, 1558
BseBI CCWGG 1 cut(s) 14
BseDI CCNNGG 4 cut(s) 13, 26, 422, 1587
BseGI GGATG 3 cut(s) 1006, 1341, 1353
BseLI CCNNNNNNNGG 3 cut(s) 27, 358, 626
BseMI GCAATG 3 cut(s) 888, 1111, 1558
BseMII CTCAG 6 cut(s) 183, 267, 482, 543, 1241, 1462
BseNI ACTGG 4 cut(s) 258, 390, 483, 1105
BseRI GAGGAG 2 cut(s) 569, 1136
BseSI GKGCMC 1 cut(s) 745
BseXI GCAGC 8 cut(s) 319, 469, 769, 911, 1096, 1273, 1303, 1522
BseYI CCCAGC 1 cut(s) 862
BshFI GGCC 5 cut(s) 362, 953, 1005, 1097, 1305
BsiHKAI GWGCWC 2 cut(s) 659, 1402
BsiSI CCGG 2 cut(s) 353, 1006
BslFI GGGAC 3 cut(s) 489, 545, 915
BslI CCNNNNNNNGG 3 cut(s) 27, 358, 626
BsmAI GTCTC 1 cut(s) 549
BsmFI GGGAC 3 cut(s) 489, 545, 915
BsnI GGCC 5 cut(s) 362, 953, 1005, 1097, 1305
Bsp1286I GDGCHC 5 cut(s) 20, 659, 745, 1030, 1402
Bsp143I GATC 4 cut(s) 397, 516, 856, 1497
Bsp1720I GCTNAGC 1 cut(s) 92
Bsp19I CCATGG 2 cut(s) 26, 1587
BspACI CCGC 1 cut(s) 1568
BspANI GGCC 5 cut(s) 362, 953, 1005, 1097, 1305
BspCNI CTCAG 6 cut(s) 184, 268, 483, 544, 1240, 1461
BspLI GGNNCC 6 cut(s) 19, 561, 930, 931, 954, 1304
BspMAI CTGCAG 2 cut(s) 759, 1162
BspTI CTTAAG 2 cut(s) 509, 934
BsrDI GCAATG 3 cut(s) 888, 1111, 1558
BsrI ACTGG 4 cut(s) 258, 390, 483, 1105
BssECI CCNNGG 4 cut(s) 13, 26, 422, 1587
BssMI GATC 4 cut(s) 397, 516, 856, 1497
BssNI GRCGYC 1 cut(s) 137
BssT1I CCWWGG 2 cut(s) 26, 1587
Bst2UI CCWGG 1 cut(s) 14
Bst4CI ACNGT 5 cut(s) 695, 853, 913, 1020, 1246
Bst6I CTCTTC 1 cut(s) 1602
BstACI GRCGYC 1 cut(s) 137
BstAFI CTTAAG 2 cut(s) 509, 934
BstAPI GCANNNNNTGC 1 cut(s) 647
BstC8I GCNNGC 3 cut(s) 387, 1355, 1549
BstDSI CCRYGG 2 cut(s) 26, 1587
BstEII GGTNACC 1 cut(s) 883
BstF5I GGATG 3 cut(s) 1006, 1341, 1353
BstHHI GCGC 1 cut(s) 337
BstKTI GATC 4 cut(s) 400, 519, 859, 1500
BstMAI GTCTC 1 cut(s) 549
BstMBI GATC 4 cut(s) 397, 516, 856, 1497
BstMWI GCNNNNNNNGC 5 cut(s) 239, 647, 899, 1094, 1544
BstNI CCWGG 1 cut(s) 14
BstPI GGTNACC 1 cut(s) 883
BstSCI CCNGG 3 cut(s) 12, 351, 1005
BstSFI CTRYAG 3 cut(s) 755, 1158, 1377
BstSLI GKGCMC 1 cut(s) 745
BstV1I GCAGC 8 cut(s) 319, 469, 769, 911, 1096, 1273, 1303, 1522
BstV2I GAAGAC 1 cut(s) 1214
BstXI CCANNNNNNTGG 1 cut(s) 1001
BsuI GTATCC 1 cut(s) 1234
BsuRI GGCC 5 cut(s) 362, 953, 1005, 1097, 1305
BtgI CCRYGG 2 cut(s) 26, 1587
BtsCI GGATG 3 cut(s) 1006, 1341, 1353
BtsI GCAGTG 1 cut(s) 1155
BtsIMutI CAGTG 3 cut(s) 488, 1098, 1155
Cac8I GCNNGC 3 cut(s) 387, 1355, 1549
CfoI GCGC 1 cut(s) 337
Cfr13I GGNCC 7 cut(s) 361, 419, 435, 559, 929, 952, 1303
CseI GACGC 1 cut(s) 126
Csp6I GTAC 3 cut(s) 251, 803, 828
CspCI CAANNNNNGTGG 2 cut(s) 1536, 1571
CviAII CATG 5 cut(s) 27, 626, 718, 995, 1588
CviQI GTAC 3 cut(s) 251, 803, 828
DpnI GATC 4 cut(s) 399, 518, 858, 1499
DpnII GATC 4 cut(s) 397, 516, 856, 1497
EaeI YGGCCR 2 cut(s) 1003, 1095
Eam1104I CTCTTC 1 cut(s) 1602
EarI CTCTTC 1 cut(s) 1602
Eco130I CCWWGG 2 cut(s) 26, 1587
Eco24I GRGCYC 2 cut(s) 20, 1030
Eco47I GGWCC 4 cut(s) 419, 435, 559, 929
Eco57I CTGAAG 3 cut(s) 70, 87, 798
Eco91I GGTNACC 1 cut(s) 883
EcoO109I RGGNCCY 4 cut(s) 559, 929, 952, 1303
EcoO65I GGTNACC 1 cut(s) 883
EcoRII CCWGG 1 cut(s) 12
EcoT14I CCWWGG 2 cut(s) 26, 1587
EcoT38I GRGCYC 2 cut(s) 20, 1030
ErhI CCWWGG 2 cut(s) 26, 1587
FaeI CATG 5 cut(s) 30, 629, 721, 998, 1591
FalI AAGNNNNNCTT 2 cut(s) 85, 117
FaqI GGGAC 3 cut(s) 489, 545, 915
FatI CATG 5 cut(s) 26, 625, 717, 994, 1587
FbaI TGATCA 2 cut(s) 397, 516
Fnu4HI GCNGC 8 cut(s) 333, 483, 783, 900, 1110, 1262, 1317, 1536
FokI GGATG 3 cut(s) 1013, 1348, 1360
FriOI GRGCYC 2 cut(s) 20, 1030
Fsp4HI GCNGC 8 cut(s) 333, 483, 783, 900, 1110, 1262, 1317, 1536
FspBI CTAG 3 cut(s) 969, 1116, 1595
GlaI GCGC 1 cut(s) 336
GluI GCNGC 8 cut(s) 333, 483, 783, 900, 1110, 1262, 1317, 1536
GsaI CCCAGC 1 cut(s) 866
GsuI CTGGAG 2 cut(s) 275, 531
HaeIII GGCC 5 cut(s) 362, 953, 1005, 1097, 1305
HapII CCGG 2 cut(s) 353, 1006
HgaI GACGC 1 cut(s) 126
HhaI GCGC 1 cut(s) 337
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 5 cut(s) 30, 629, 721, 998, 1591
Hin6I GCGC 1 cut(s) 335
HinP1I GCGC 1 cut(s) 335
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HindIII AAGCTT 1 cut(s) 94
HinfI GANTC 6 cut(s) 182, 750, 775, 1010, 1216, 1520
HpaII CCGG 2 cut(s) 353, 1006
HphI GGTGA 2 cut(s) 310, 877
Hpy166II GTNNAC 4 cut(s) 109, 457, 790, 830
Hpy188III TCNNGA 6 cut(s) 438, 1116, 1333, 1427, 1501, 1604
Hpy8I GTNNAC 4 cut(s) 109, 457, 790, 830
Hpy99I CGWCG 1 cut(s) 142
HpyAV CCTTC 2 cut(s) 822, 1424
HpyCH4III ACNGT 5 cut(s) 695, 853, 913, 1020, 1246
HpyCH4IV ACGT 1 cut(s) 1049
HpyCH4V TGCA 8 cut(s) 176, 485, 614, 650, 701, 757, 899, 1160
HpyF10VI GCNNNNNNNGC 5 cut(s) 239, 647, 899, 1094, 1544
HpySE526I ACGT 1 cut(s) 1049
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 5 cut(s) 30, 629, 721, 998, 1591
HspAI GCGC 1 cut(s) 335
KflI GGGWCCC 1 cut(s) 929
Ksp22I TGATCA 2 cut(s) 397, 516
Kzo9I GATC 4 cut(s) 397, 516, 856, 1497
LmnI GCTCC 3 cut(s) 23, 662, 907
Lsp1109I GCAGC 8 cut(s) 319, 469, 769, 911, 1096, 1273, 1303, 1522
LweI GCATC 5 cut(s) 229, 493, 494, 650, 710
MaeI CTAG 3 cut(s) 969, 1116, 1595
MaeII ACGT 1 cut(s) 1049
MaeIII GTNAC 5 cut(s) 883, 1011, 1153, 1162, 1183
MalI GATC 4 cut(s) 399, 518, 858, 1499
MboI GATC 4 cut(s) 397, 516, 856, 1497
MboII GAAGA 7 cut(s) 76, 719, 1214, 1467, 1482, 1495, 1619
MfeI CAATTG 2 cut(s) 1080, 1211
MhlI GDGCHC 5 cut(s) 20, 659, 745, 1030, 1402
MlsI TGGCCA 1 cut(s) 1097
MluCI AATT 9 cut(s) 356, 468, 958, 1080, 1211, 1441, 1458, 1514, 1685
MluNI TGGCCA 1 cut(s) 1097
MlyI GAGTC 3 cut(s) 176, 1019, 1514
MmeI TCCRAC 1 cut(s) 1159
Mox20I TGGCCA 1 cut(s) 1097
MroXI GAANNNNTTC 1 cut(s) 1653
MscI TGGCCA 1 cut(s) 1097
MseI TTAA 5 cut(s) 215, 467, 510, 935, 1022
MslI CAYNNNNRTG 1 cut(s) 999
Msp20I TGGCCA 1 cut(s) 1097
MspA1I CMGCKG 1 cut(s) 551
MspCI CTTAAG 2 cut(s) 509, 934
MspI CCGG 2 cut(s) 353, 1006
MspR9I CCNGG 3 cut(s) 14, 353, 1007
MunI CAATTG 2 cut(s) 1080, 1211
MvaI CCWGG 1 cut(s) 14
MwoI GCNNNNNNNGC 5 cut(s) 239, 647, 899, 1094, 1544
NciI CCSGG 2 cut(s) 353, 1007
NcoI CCATGG 2 cut(s) 26, 1587
NdeII GATC 4 cut(s) 397, 516, 856, 1497
NlaIII CATG 5 cut(s) 30, 629, 721, 998, 1591
NlaIV GGNNCC 6 cut(s) 19, 561, 930, 931, 954, 1304
NmeAIII GCCGAG 2 cut(s) 308, 557
NmuCI GTSAC 3 cut(s) 883, 1011, 1153
PdmI GAANNNNTTC 1 cut(s) 1653
PfeI GAWTC 3 cut(s) 750, 775, 1216
PflMI CCANNNNNTGG 1 cut(s) 626
PkrI GCNGC 8 cut(s) 334, 484, 784, 901, 1111, 1263, 1318, 1537
PleI GAGTC 3 cut(s) 176, 1018, 1514
PpsI GAGTC 3 cut(s) 176, 1018, 1514
PpuMI RGGWCCY 2 cut(s) 559, 929
PshBI ATTAAT 1 cut(s) 467
Psp5II RGGWCCY 2 cut(s) 559, 929
Psp6I CCWGG 1 cut(s) 12
PspEI GGTNACC 1 cut(s) 883
PspFI CCCAGC 1 cut(s) 862
PspGI CCWGG 1 cut(s) 12
PspN4I GGNNCC 6 cut(s) 19, 561, 930, 931, 954, 1304
PspPI GGNCC 7 cut(s) 361, 419, 435, 559, 929, 952, 1303
PspPPI RGGWCCY 2 cut(s) 559, 929
PstI CTGCAG 2 cut(s) 759, 1162
PvuII CAGCTG 1 cut(s) 551
RsaI GTAC 3 cut(s) 252, 804, 829
RsaNI GTAC 3 cut(s) 251, 803, 828
RseI CAYNNNNRTG 1 cut(s) 999
SaqAI TTAA 5 cut(s) 215, 467, 510, 935, 1022
SatI GCNGC 8 cut(s) 333, 483, 783, 900, 1110, 1262, 1317, 1536
Sau3AI GATC 4 cut(s) 397, 516, 856, 1497
Sau96I GGNCC 7 cut(s) 361, 419, 435, 559, 929, 952, 1303
ScaI AGTACT 1 cut(s) 252
SchI GAGTC 3 cut(s) 176, 1019, 1514
ScrFI CCNGG 3 cut(s) 14, 353, 1007
SduI GDGCHC 5 cut(s) 20, 659, 745, 1030, 1402
SfaNI GCATC 5 cut(s) 229, 493, 494, 650, 710
SfcI CTRYAG 3 cut(s) 755, 1158, 1377
SinI GGWCC 4 cut(s) 419, 435, 559, 929
SmiMI CAYNNNNRTG 1 cut(s) 999
SmlI CTYRAG 3 cut(s) 509, 934, 1358
SmoI CTYRAG 3 cut(s) 509, 934, 1358
Sse9I AATT 9 cut(s) 356, 468, 958, 1080, 1211, 1441, 1458, 1514, 1685
SsiI CCGC 1 cut(s) 1568
SspI AATATT 1 cut(s) 1564
SspMI CTAG 3 cut(s) 969, 1116, 1595
StyD4I CCNGG 3 cut(s) 12, 351, 1005
StyI CCWWGG 2 cut(s) 26, 1587
TaaI ACNGT 5 cut(s) 695, 853, 913, 1020, 1246
TaiI ACGT 1 cut(s) 1052
TaqI TCGA 3 cut(s) 1426, 1493, 1500
TasI AATT 9 cut(s) 356, 468, 958, 1080, 1211, 1441, 1458, 1514, 1685
TatI WGTACW 1 cut(s) 250
TfiI GAWTC 3 cut(s) 750, 775, 1216
Tru1I TTAA 5 cut(s) 215, 467, 510, 935, 1022
Tru9I TTAA 5 cut(s) 215, 467, 510, 935, 1022
TscAI CASTG 3 cut(s) 495, 1105, 1162
TseFI GTSAC 3 cut(s) 883, 1011, 1153
TseI GCWGC 8 cut(s) 332, 482, 782, 899, 1109, 1261, 1316, 1535
Tsp45I GTSAC 3 cut(s) 883, 1011, 1153
TspDTI ATGAA 4 cut(s) 282, 393, 426, 684
TspGWI ACGGA 1 cut(s) 1141
TspRI CASTG 3 cut(s) 495, 1105, 1162
Van91I CCANNNNNTGG 1 cut(s) 626
Vha464I CTTAAG 2 cut(s) 509, 934
VpaK11BI GGWCC 4 cut(s) 419, 435, 559, 929
VspI ATTAAT 1 cut(s) 467
XapI RAATTY 1 cut(s) 1441
XbaI TCTAGA 1 cut(s) 1115
XmnI GAANNNNTTC 1 cut(s) 1653
XspI CTAG 3 cut(s) 969, 1116, 1595
ZrmI AGTACT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.