Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0008591

Physical Location & Seq

Genomic Coordinates Forward (+)

15566492 .. 15570384

Gene Structure 3893 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom06g10180.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1650 bp

ATGACTCTAATAAACCTCAGCTCCCTCTCCCCATGGCTCTCCCGCCTCTCTCACCACCCTTCTCCGCCCCTTCCCATCTTTCCCACCACCACTTTTTTGTTCAGAAATATTCAATCCACTTCCATCAACTTCTCAAAGCTCCTCAGAGTAAAAGCCTCATTGGGGGAGACCCAAAACGGTAGTGGGTTGGCAAAGGACTCTGTTTCCGAGTTGTTAGATGACGAGTTGCTTAGTGGAGTTTCCGGTGCTAAAGATGCCCAGGAAGCACTCCAGGTGATCGCTGAAATGTCTGACAGAAATGGTGGTTTAGTGAGTGTATCTGATTGTTGTGCTATTATATCGGCTGCCCTAAAGCGGAACAACTCCGACTTGGCTCTCTCTGTTTTCTATGAAATGCGTGCGAGTTTTGATCAAGGTGTTAATGAAAATGGCCCTCTTGTTGAGAGGTGGAAATGGTCCAGACCAGATGTGCATGTTTACACATCGTTGATTCTGGGTCTAGCTGCATCCCTTAGGGTTTCTGATGCCCTTAGGATGATCAACAATGTTTGCCGAGTTGGAGTATCTCCTGCTGAGGAGGTCCCATTTGGGAAGGTTGTTAGGTGTCCTAGCTGTATGATAGCTGTTGCTGTTGCACAGCCACAACATGGTATTCAGGTAGTGTCGTGTGCAAAGTGCTGCTACCAGTATGAACTTATTTCTGGCAACATAGCTAGTATTGAGTCGGAAGAAATCAGCATGGATGTTCCGGCTTGGAAAAGGGGACTAAGATTCCTGAACATAATGAAGCAAAGTATTCCTGCGGCTGTCCACTCCATTGTGGTACAAACCCCTTCTGGTATGGCACGTACAAACAGGTTTGCTACTGAAACAGTTGATCTCCCAGCACAGGAAGGGGAAAGGGTAACGATTGCTCTAGCTGCTCCATTAAGTGTTTACAGAGAGGTCGGTCCCTTAAAATTTAGTCCTAAGGCCCCAAATTTTTACCCTGGGGAGCCTATCTGCTTGACAAACCATGAAGATGACCGGGAGTCAAAACTATTAAGAGCCCCTGTAAAGGATGGAGGACCGTTGCTAACTAACCCCTCCATCCTTTTTCCAATTGTTGCGGTTTTGGCCTCTGGAAATGCCGCCTCCGGAGTTATAGACCCCATCTTCCCCCAATTCCTTCCAGTTGCTGCAGTAGCGTCTCTTGCTGTTGGAGTTACTCTGAACAAATTGGTTGTCCCCCAATTAAATCGTCTTCCTCGGAGAATAGTGGATACAGTTGCCATCAAGCAGCAACTTTTATCGCAATATGACACACTCCAATCTCGTATAAAGGGCCTAAAAGAATCTGCTGAAAAAGAGGTCTGGATGTTGGCTCGGATGTGCCAACTTGAGAACAAAATCGTTGCTGTAGGAGAGCCTTCTTATCGTGCTCGAAGAAGTAAAGTCAAAAGGGTTCGAGAAGGCTTGGAAAATTCTCTCAGGGGACGGATTGAACTTATTGACAGCTATGCAAGGATTTCTTCCATGATTGAAATTGAGGTAGAACTGGACTCCGATGTTCTTGCTGCCGAAGCAGCAAGCACACTGGAAAATGTTGCTGAACAGATACAGCAAACCATGGAGCTAGAAAATCTTGAAGAGATGGAGAATACAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

550

Amino Acids

59.93

Weight (kDa)

5.75

Isoelectric Point (pI)

50.01

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008591)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64430 AT1G64430 AT1G64430
fragaria_vesca	FvH4_5g04720 FvH4_5g04720
malus_domestica	MD06G1108400.v1.1
prunus_persica	Prupe.5G121000_v2.0.a1 Prupe.5G121000_v2.0.a1
pyrus_communis	pycom06g10180
rosa_chinensis	RchiOBHm_Chr7g0194681
rosa_laevigata	RLG00000004182
rosa_multiflora	Rmu_sc0018264.1_g000003 Rmu_sc0023803.1_g000001 Rmu_sc0034112.1_g000001
rosa_roxburghii	Rroxscaffold_3G00261160
rosa_rugosa	Rorug07G0017200 Rorug07G0017300 Rorug07G0017300
rosa_samantha	Rh7AG144700 Rh7BG144700 Rh7CG148500 Rh7DG145900
rosa_wichuraiana	Rw7G012360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	647
AccIII	TCCGGA	1 cut(s)	1136
AciI	CCGC	6 cut(s)	43, 65, 355, 803, 1109, 1131
AcsI	RAATTY	3 cut(s)	959, 979, 1462
AfaI	GTAC	2 cut(s)	825, 850
AfiI	CCNNNNNNNGG	5 cut(s)	162, 354, 647, 889, 1057
AgsI	TTSAA	4 cut(s)	113, 1484, 1523, 1628
AhdI	GACNNNNNGTC	1 cut(s)	1030
AjnI	CCWGG	3 cut(s)	258, 270, 988
AluBI	AGCT	9 cut(s)	21, 139, 503, 612, 623, 713, 920, 1497, 1615
AluI	AGCT	9 cut(s)	21, 139, 503, 612, 623, 713, 920, 1497, 1615
Alw21I	GWGCWC	1 cut(s)	1423
Alw26I	GTCTC	2 cut(s)	161, 1194
AlwNI	CAGNNNCTG	1 cut(s)	1178
Aor13HI	TCCGGA	1 cut(s)	1136
AoxI	GGCC	4 cut(s)	430, 972, 1116, 1324
ApeKI	GCWGC	8 cut(s)	344, 503, 678, 920, 1178, 1279, 1556, 1565
ApoI	RAATTY	3 cut(s)	959, 979, 1462
ArsI	GACNNNNNNTTYG	2 cut(s)	1209, 1241
AspS9I	GGNCC	7 cut(s)	431, 456, 580, 950, 973, 1067, 1324
AsuC2I	CCSGG	1 cut(s)	1028
AsuHPI	GGTGA	2 cut(s)	44, 286
AvaII	GGWCC	4 cut(s)	456, 580, 950, 1067
AxyI	CCTNAGG	3 cut(s)	512, 530, 969
BanII	GRGCYC	1 cut(s)	1051
BbsI	GAAGAC	1 cut(s)	1235
Bbv12I	GWGCWC	1 cut(s)	1423
BbvCI	CCTCAGC	2 cut(s)	17, 573
BbvI	GCAGC	8 cut(s)	331, 490, 665, 907, 1165, 1291, 1543, 1577
BccI	CCATC	7 cut(s)	83, 131, 1055, 1097, 1160, 1280, 1627
BcgI	CGANNNNNNTGC	2 cut(s)	1535, 1569
BciT130I	CCWGG	3 cut(s)	260, 272, 990
BciVI	GTATCC	1 cut(s)	1255
BclI	TGATCA	2 cut(s)	409, 537
BcnI	CCSGG	1 cut(s)	1028
BcoDI	GTCTC	2 cut(s)	161, 1194
BfaI	CTAG	5 cut(s)	500, 609, 714, 917, 1616
BfmI	CTRYAG	2 cut(s)	1179, 1398
BfuI	GTATCC	1 cut(s)	1255
Bme1390I	CCNGG	4 cut(s)	260, 272, 990, 1028
Bme18I	GGWCC	4 cut(s)	456, 580, 950, 1067
BmeRI	GACNNNNNGTC	1 cut(s)	1030
BmgT120I	GGNCC	7 cut(s)	431, 456, 580, 950, 973, 1067, 1324
BmiI	GGNNCC	4 cut(s)	582, 952, 975, 996
BmrFI	CCNGG	4 cut(s)	260, 272, 990, 1028
BmsI	GCATC	3 cut(s)	244, 514, 515
BpiI	GAAGAC	1 cut(s)	1235
BpmI	CTGGAG	1 cut(s)	254
Bpu10I	CCTNAGC	2 cut(s)	17, 573
BpuEI	CTTGAG	1 cut(s)	1400
BpuMI	CCSGG	1 cut(s)	1028
BsaAI	YACGTR	1 cut(s)	848
BsaI	GGTCTC	1 cut(s)	161
BsaJI	CCNNGG	6 cut(s)	32, 258, 988, 989, 1247, 1608
BsaWI	WCCGGW	2 cut(s)	242, 1136
BsaXI	ACNNNNNCTCC	2 cut(s)	5, 35
Bsc4I	CCNNNNNNNGG	5 cut(s)	162, 354, 647, 889, 1057
Bse1I	ACTGG	4 cut(s)	685, 1172, 1542, 1581
Bse21I	CCTNAGG	3 cut(s)	512, 530, 969
BseAI	TCCGGA	1 cut(s)	1136
BseBI	CCWGG	3 cut(s)	260, 272, 990
BseDI	CCNNGG	6 cut(s)	32, 258, 988, 989, 1247, 1608
BseGI	GGATG	7 cut(s)	506, 540, 748, 1066, 1089, 1362, 1374
BseLI	CCNNNNNNNGG	5 cut(s)	162, 354, 647, 889, 1057
BseMII	CTCAG	4 cut(s)	31, 157, 564, 1483
BseNI	ACTGG	4 cut(s)	685, 1172, 1542, 1581
BseRI	GAGGAG	2 cut(s)	131, 590
BseXI	GCAGC	8 cut(s)	331, 490, 665, 907, 1165, 1291, 1543, 1577
BseYI	CCCAGC	1 cut(s)	883
BshFI	GGCC	4 cut(s)	432, 974, 1118, 1326
BsiHKAI	GWGCWC	1 cut(s)	1423
BsiSI	CCGG	4 cut(s)	243, 749, 1027, 1137
BslFI	GGGAC	5 cut(s)	566, 777, 936, 1211, 1488
BslI	CCNNNNNNNGG	5 cut(s)	162, 354, 647, 889, 1057
BsmAI	GTCTC	2 cut(s)	161, 1194
BsmBI	CGTCTC	1 cut(s)	1194
BsmFI	GGGAC	5 cut(s)	566, 777, 936, 1211, 1488
BsnI	GGCC	4 cut(s)	432, 974, 1118, 1326
Bso31I	GGTCTC	1 cut(s)	161
Bsp1286I	GDGCHC	2 cut(s)	1051, 1423
Bsp13I	TCCGGA	1 cut(s)	1136
Bsp143I	GATC	4 cut(s)	276, 409, 537, 877
Bsp19I	CCATGG	2 cut(s)	32, 1608
BspACI	CCGC	6 cut(s)	43, 65, 355, 803, 1109, 1131
BspANI	GGCC	4 cut(s)	432, 974, 1118, 1326
BspCNI	CTCAG	4 cut(s)	30, 156, 565, 1482
BspEI	TCCGGA	1 cut(s)	1136
BspLI	GGNNCC	4 cut(s)	582, 952, 975, 996
BspMAI	CTGCAG	1 cut(s)	1183
BspTNI	GGTCTC	1 cut(s)	161
BsrI	ACTGG	4 cut(s)	685, 1172, 1542, 1581
BssECI	CCNNGG	6 cut(s)	32, 258, 988, 989, 1247, 1608
BssMI	GATC	4 cut(s)	276, 409, 537, 877
BssT1I	CCWWGG	2 cut(s)	32, 1608
Bst2UI	CCWGG	3 cut(s)	260, 272, 990
Bst4CI	ACNGT	4 cut(s)	179, 874, 1071, 1267
Bst6I	CTCTTC	1 cut(s)	1623
BstBAI	YACGTR	1 cut(s)	848
BstC8I	GCNNGC	2 cut(s)	399, 1570
BstDEI	CTNAG	9 cut(s)	17, 143, 230, 512, 530, 573, 767, 969, 1469
BstDSI	CCRYGG	2 cut(s)	32, 1608
BstF5I	GGATG	7 cut(s)	506, 540, 748, 1066, 1089, 1362, 1374
BstKTI	GATC	4 cut(s)	279, 412, 540, 880
BstMAI	GTCTC	2 cut(s)	161, 1194
BstMBI	GATC	4 cut(s)	276, 409, 537, 877
BstMWI	GCNNNNNNNGC	8 cut(s)	254, 263, 920, 1115, 1184, 1193, 1562, 1565
BstNI	CCWGG	3 cut(s)	260, 272, 990
BstNSI	RCATGY	1 cut(s)	476
BstSCI	CCNGG	4 cut(s)	258, 270, 988, 1026
BstSFI	CTRYAG	2 cut(s)	1179, 1398
BstV1I	GCAGC	8 cut(s)	331, 490, 665, 907, 1165, 1291, 1543, 1577
BstV2I	GAAGAC	1 cut(s)	1235
Bsu36I	CCTNAGG	3 cut(s)	512, 530, 969
BsuI	GTATCC	1 cut(s)	1255
BsuRI	GGCC	4 cut(s)	432, 974, 1118, 1326
BtgI	CCRYGG	2 cut(s)	32, 1608
BtsCI	GGATG	7 cut(s)	506, 540, 748, 1066, 1089, 1362, 1374
BtsIMutI	CAGTG	1 cut(s)	1574
Cac8I	GCNNGC	2 cut(s)	399, 1570
CaiI	CAGNNNCTG	1 cut(s)	1178
Cfr13I	GGNCC	7 cut(s)	431, 456, 580, 950, 973, 1067, 1324
CseI	GACGC	1 cut(s)	1176
Csp6I	GTAC	2 cut(s)	824, 849
CviAII	CATG	7 cut(s)	33, 473, 647, 739, 1016, 1516, 1609
CviQI	GTAC	2 cut(s)	824, 849
DdeI	CTNAG	9 cut(s)	17, 143, 230, 512, 530, 573, 767, 969, 1469
DpnI	GATC	4 cut(s)	278, 411, 539, 879
DpnII	GATC	4 cut(s)	276, 409, 537, 877
DriI	GACNNNNNGTC	1 cut(s)	1030
Eam1104I	CTCTTC	1 cut(s)	1623
Eam1105I	GACNNNNNGTC	1 cut(s)	1030
EarI	CTCTTC	1 cut(s)	1623
EciI	GGCGGA	1 cut(s)	54
Eco130I	CCWWGG	2 cut(s)	32, 1608
Eco24I	GRGCYC	1 cut(s)	1051
Eco31I	GGTCTC	1 cut(s)	161
Eco47I	GGWCC	4 cut(s)	456, 580, 950, 1067
Eco81I	CCTNAGG	3 cut(s)	512, 530, 969
EcoO109I	RGGNCCY	3 cut(s)	580, 973, 1324
EcoRII	CCWGG	3 cut(s)	258, 270, 988
EcoT14I	CCWWGG	2 cut(s)	32, 1608
EcoT38I	GRGCYC	1 cut(s)	1051
ErhI	CCWWGG	2 cut(s)	32, 1608
Esp3I	CGTCTC	1 cut(s)	1194
FaeI	CATG	7 cut(s)	36, 476, 650, 742, 1019, 1519, 1612
FalI	AAGNNNNNCTT	2 cut(s)	1495, 1527
FaqI	GGGAC	5 cut(s)	566, 777, 936, 1211, 1488
FatI	CATG	7 cut(s)	32, 472, 646, 738, 1015, 1515, 1608
FauI	CCCGC	1 cut(s)	50
FbaI	TGATCA	2 cut(s)	409, 537
FokI	GGATG	7 cut(s)	493, 547, 755, 1073, 1076, 1369, 1381
FriOI	GRGCYC	1 cut(s)	1051
FspBI	CTAG	5 cut(s)	500, 609, 714, 917, 1616
GsaI	CCCAGC	1 cut(s)	887
GsuI	CTGGAG	1 cut(s)	254
HaeIII	GGCC	4 cut(s)	432, 974, 1118, 1326
HapII	CCGG	4 cut(s)	243, 749, 1027, 1137
HgaI	GACGC	1 cut(s)	1176
Hin1II	CATG	7 cut(s)	36, 476, 650, 742, 1019, 1519, 1612
HinfI	GANTC	8 cut(s)	4, 197, 490, 722, 771, 1031, 1334, 1541
HpaII	CCGG	4 cut(s)	243, 749, 1027, 1137
HphI	GGTGA	2 cut(s)	44, 286
Hpy166II	GTNNAC	3 cut(s)	478, 811, 937
Hpy188III	TCNNGA	7 cut(s)	459, 775, 1122, 1137, 1354, 1448, 1625
Hpy8I	GTNNAC	3 cut(s)	478, 811, 937
HpyAV	CCTTC	8 cut(s)	69, 80, 586, 843, 887, 1178, 1419, 1445
HpyCH4III	ACNGT	4 cut(s)	179, 874, 1071, 1267
HpyCH4IV	ACGT	1 cut(s)	847
HpyCH4V	TGCA	6 cut(s)	472, 506, 635, 671, 1181, 1502
HpyF10VI	GCNNNNNNNGC	8 cut(s)	254, 263, 920, 1115, 1184, 1193, 1562, 1565
HpyF3I	CTNAG	9 cut(s)	17, 143, 230, 512, 530, 573, 767, 969, 1469
HpySE526I	ACGT	1 cut(s)	847
Hsp92II	CATG	7 cut(s)	36, 476, 650, 742, 1019, 1519, 1612
Kpn2I	TCCGGA	1 cut(s)	1136
Ksp22I	TGATCA	2 cut(s)	409, 537
Kzo9I	GATC	4 cut(s)	276, 409, 537, 877
LmnI	GCTCC	5 cut(s)	26, 144, 928, 994, 1612
Lsp1109I	GCAGC	8 cut(s)	331, 490, 665, 907, 1165, 1291, 1543, 1577
LweI	GCATC	3 cut(s)	244, 514, 515
MaeI	CTAG	5 cut(s)	500, 609, 714, 917, 1616
MaeII	ACGT	1 cut(s)	847
MaeIII	GTNAC	2 cut(s)	904, 1204
MalI	GATC	4 cut(s)	278, 411, 539, 879
MboI	GATC	4 cut(s)	276, 409, 537, 877
MboII	GAAGA	7 cut(s)	740, 1031, 1147, 1235, 1437, 1503, 1640
MfeI	CAATTG	1 cut(s)	1101
MhlI	GDGCHC	2 cut(s)	1051, 1423
MluCI	AATT	8 cut(s)	959, 979, 1101, 1163, 1217, 1232, 1462, 1524
MlyI	GAGTC	4 cut(s)	191, 731, 1040, 1535
MmeI	TCCRAC	4 cut(s)	390, 538, 705, 1180
MroI	TCCGGA	1 cut(s)	1136
MseI	TTAA	5 cut(s)	420, 929, 956, 1043, 1235
MslI	CAYNNNNRTG	1 cut(s)	1020
MspI	CCGG	4 cut(s)	243, 749, 1027, 1137
MspR9I	CCNGG	4 cut(s)	260, 272, 990, 1028
MunI	CAATTG	1 cut(s)	1101
MvaI	CCWGG	3 cut(s)	260, 272, 990
MwoI	GCNNNNNNNGC	8 cut(s)	254, 263, 920, 1115, 1184, 1193, 1562, 1565
NciI	CCSGG	1 cut(s)	1028
NcoI	CCATGG	2 cut(s)	32, 1608
NdeII	GATC	4 cut(s)	276, 409, 537, 877
NlaIII	CATG	7 cut(s)	36, 476, 650, 742, 1019, 1519, 1612
NlaIV	GGNNCC	4 cut(s)	582, 952, 975, 996
NmeAIII	GCCGAG	1 cut(s)	578
NspI	RCATGY	1 cut(s)	476
PasI	CCCWGGG	1 cut(s)	989
PfeI	GAWTC	3 cut(s)	490, 771, 1334
PflMI	CCANNNNNTGG	1 cut(s)	647
PleI	GAGTC	4 cut(s)	191, 730, 1039, 1535
PpsI	GAGTC	4 cut(s)	191, 730, 1039, 1535
Ppu21I	YACGTR	1 cut(s)	848
PpuMI	RGGWCCY	1 cut(s)	580
Psp5II	RGGWCCY	1 cut(s)	580
Psp6I	CCWGG	3 cut(s)	258, 270, 988
PspFI	CCCAGC	1 cut(s)	883
PspGI	CCWGG	3 cut(s)	258, 270, 988
PspN4I	GGNNCC	4 cut(s)	582, 952, 975, 996
PspPI	GGNCC	7 cut(s)	431, 456, 580, 950, 973, 1067, 1324
PspPPI	RGGWCCY	1 cut(s)	580
PstI	CTGCAG	1 cut(s)	1183
PstNI	CAGNNNCTG	1 cut(s)	1178
RsaI	GTAC	2 cut(s)	825, 850
RsaNI	GTAC	2 cut(s)	824, 849
RseI	CAYNNNNRTG	1 cut(s)	1020
SaqAI	TTAA	5 cut(s)	420, 929, 956, 1043, 1235
Sau3AI	GATC	4 cut(s)	276, 409, 537, 877
Sau96I	GGNCC	7 cut(s)	431, 456, 580, 950, 973, 1067, 1324
SchI	GAGTC	4 cut(s)	191, 731, 1040, 1535
ScrFI	CCNGG	4 cut(s)	260, 272, 990, 1028
SduI	GDGCHC	2 cut(s)	1051, 1423
SfaNI	GCATC	3 cut(s)	244, 514, 515
SfcI	CTRYAG	2 cut(s)	1179, 1398
SinI	GGWCC	4 cut(s)	456, 580, 950, 1067
SmiMI	CAYNNNNRTG	1 cut(s)	1020
SmlI	CTYRAG	1 cut(s)	1379
SmoI	CTYRAG	1 cut(s)	1379
Sse9I	AATT	8 cut(s)	959, 979, 1101, 1163, 1217, 1232, 1462, 1524
SsiI	CCGC	6 cut(s)	43, 65, 355, 803, 1109, 1131
SspI	AATATT	1 cut(s)	109
SspMI	CTAG	5 cut(s)	500, 609, 714, 917, 1616
StyD4I	CCNGG	4 cut(s)	258, 270, 988, 1026
StyI	CCWWGG	2 cut(s)	32, 1608
TaaI	ACNGT	4 cut(s)	179, 874, 1071, 1267
TaiI	ACGT	1 cut(s)	850
TaqI	TCGA	2 cut(s)	1423, 1447
TaqII	GACCGA	1 cut(s)	938
TasI	AATT	8 cut(s)	959, 979, 1101, 1163, 1217, 1232, 1462, 1524
TauI	GCSGC	2 cut(s)	806, 1133
TfiI	GAWTC	3 cut(s)	490, 771, 1334
Tru1I	TTAA	5 cut(s)	420, 929, 956, 1043, 1235
Tru9I	TTAA	5 cut(s)	420, 929, 956, 1043, 1235
TscAI	CASTG	1 cut(s)	1581
TseI	GCWGC	8 cut(s)	344, 503, 678, 920, 1178, 1279, 1556, 1565
TspDTI	ATGAA	5 cut(s)	405, 438, 705, 800, 1032
TspGWI	ACGGA	1 cut(s)	1492
TspRI	CASTG	1 cut(s)	1581
Van91I	CCANNNNNTGG	1 cut(s)	647
VpaK11BI	GGWCC	4 cut(s)	456, 580, 950, 1067
XapI	RAATTY	3 cut(s)	959, 979, 1462
XceI	RCATGY	1 cut(s)	476
XcmI	CCANNNNNNNNNTGG	1 cut(s)	179
XspI	CTAG	5 cut(s)	500, 609, 714, 917, 1616

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.