Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0004752

Physical Location & Seq

Genomic Coordinates Forward (+)

3615724 .. 3620699

Gene Structure 4976 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g06130.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2112 bp

ATGGCAAGAACAACTCTTCCGGCTGTTCTCAAGGTCCTCATGGCTTTGATATTTGCTGCTTGGGTTTCCCTTTGGCTTATCAAGCCAACTCAGTTTTGGACAAGAGAATGGAAAGCAGCTGAAGATTTAGCAAGGCCTACTTTGTTTGGATATTATGGTCTTAATTTTGCTGTGTATATATTCCCTCTTTTTGCTCTGGCTATTCTTGGTTCTATATACTTGAATTTGAAGCCCAGGGAGCCAATTAGGAGAAAAGGGAGGAGAGTTTTTGCTGGTTTCTCAAATCCATTGGTGGTGAACAGTCTTCTGGGAATTTTATCTAGTATTGAAATTGTTGCTGTCTTCCTCTTCATGCTCTTCTTAGCTTGGACTTTCTATGTCCGAATTTCAAGTGATTTCAAGAAATTAAAGCCTGACAAAGCACTTAAACTGAATCTATGGCAACTCAAGTACCTAAGGGTGGCAACTCGGTTCGGTTTGTTAGCAGAAGCCTGCCTGGCTTTTCTTCTTCTCCCTGTACTAAGAGGTTTAGCCGTATTTCGGATACTTGGCATTCAGTTTGAAGCTTCAATAAGATATCATATCTGGCTTGGGACTACAATGCTATTTTGTGCTACTTTCCATGGTGTAAGCACCTTGTTCATTTGGGGGGTCAGCCATTATATTCAAGATGAGATGTGGAAATGGCAGAATACTGGGCGAATATACCTTGCTGGGGAAATCTGTCTGCTTACAGGGTTAGTGATTTGGATCACAGCACTGCCTCAAATAAGGAGAAGACAATTTGAGATATTTTACTACACACACCATCTCTACATGGTTTTCCTATTGTTCTTCTTGTTTCATGCCGGAGATCGGCACTTCTACACCGTCTTCCCTGGAATATTTCTGTTTGGACTAGACAAGCTACTTAGGATCATACAGTCTAGACCAGAAACTTGCATTCTATCAGCAAGAGTCTTCCCCTCTAAAGCTATAGAGCTTATACTACCAAAAGATCCAGGACTCAAGTATACGCCCACAAGTGTCCTATTTCTGAAGGTACCAAGTATATCTAAATATCAGTGGCACTCATTCAGCATCATATCCAGTTCTAGTGTTGATGCCAATTCAATGTCTATCCTGATTAAATCCGAAGGATCCTGGACAAGTTCTCTCTTGAATTTGATACAAACCGAGCAGAAGTCAGATGCTGATCACATGAAATGCATACCTGTTGCAGCAGAAGGTCCTTATGGACCTGCCTCAATGGACTTCCTAAGGTATGACAGTCTACTTCTTGTTGCTGGAGGAATTGGGATAACCCCATTTCTGAGCATCTTACAGGAACTTGTTGCAGCACAAAATAGCGGCCGATACAAATTCCTACCTAAAATACAACTTATATTTGCTGTCAAGAAGGTCCAAGATATATGTCTATTGAACTCAATTTCACCTCTAATCTTCAACCAGCCAGTTGAAAAGTGCCATCTGAAAGTGAAGGTATATGTGACTCAAGAAGAGCAATCTGGTGTCACAGTAAGAGAAGCACTACTATATGAGTCTTCTCGAGTGCAAACAGTAAACTTTGACATCAAATCTTCGAATCATGCAATAACTGGACTCGAAAGTCCACTCTGGATGGCTGCCGTAGCTGGAATTTCCTGTATCCTGTTTCTCATTTTTCTCATCATTTTCAACCACATTTTCATTCCCTCCCAGGAAAAGGCTACCAAAGGAAAGACTCCTTCTTTGGTTGTAGATCTGCTCATCATATGTTCTTTCATCGCAGTGCTCATCTGCATCATCTTTGTACCACTTTTTATAAGATGGAGAAGACTAAAGAAACAGAACCCTCCAGTATTCCCAAAACAAGGCAAAGTATTGGAAATAAGTCCAGCAGAAGCAAGGGATACACTTGAGGAACATGAAATCCATTTTGGAAGAAGGCCTAATTTTGAAGAGATCTTTGCAAAATTTCCAAATGAGATTGGTGGATCTGATATTGGAGTCTTGGTCTGTGGACCTGAAGCTATGAAAGAATCAGTGGCATCAATATGCCAGCATAAGTGTGGAGGTTTAAAAGCGCAGAAAGCCAATTTCAGTTTTCACTCTCTCAGTTTTGCACTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000293 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005886 GO:0008150 GO:0008152 GO:0016020 GO:0016491 GO:0016722 GO:0016723 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_8 Ferric_reduct NAD_binding_6

Protein Analysis

704

Amino Acids

79.66

Weight (kDa)

9.45

Isoelectric Point (pI)

43.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ferric_reduct	PF01794	158 - 279	1.4e-19	Ferric reductase like transmembrane component
FAD_binding_8	PF08022	316 - 416	3.3e-18	FAD-binding domain
NAD_binding_6	PF08030	422 - 681	5.8e-21	Ferric reductase NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004752)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G50160 AT5G50160
fragaria_vesca	FvH4_5g06130 FvH4_5g06130 FvH4_5g06130
malus_domestica	MD06G1166400.v1.1
prunus_persica	Prupe.5G166400_v2.0.a1 Prupe.5G166400_v2.0.a1 Prupe.5G166400_v2.0.a1
pyrus_communis	pycom06g14910
rosa_chinensis	RchiOBHm_Chr7g0192661 RchiOBHm_Chr7g0192671 RchiOBHm_Chr7g0192681
rosa_laevigata	RLG00000004337 RLG00000004363 RLG00000004456
rosa_multiflora	Rmu_sc0002040.1_g000005
rosa_roxburghii	Rroxscaffold_3G00262750
rosa_rugosa	Rorug07G0003700 Rorug07G0003800
rosa_samantha	Rh7AG128600 Rh7AG128800 Rh7BG130200 Rh7BG130300 Rh7CG133400 Rh7CG133500 Rh7CG133600 Rh7CG133700 Rh7DG131100
rosa_wichuraiana	Rw7G011000 Rw7G011020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1805
AasI	GACNNNNNNGTC	1 cut(s)	1608
Acc36I	ACCTGC	1 cut(s)	1249
Acc65I	GGTACC	1 cut(s)	1042
AccB1I	GGYRCC	1 cut(s)	1042
AccI	GTMKAC	2 cut(s)	1013, 1273
AciI	CCGC	1 cut(s)	1350
AclWI	GGATC	6 cut(s)	758, 923, 992, 1134, 1147, 1984
AcoI	YGGCCR	1 cut(s)	1351
AcsI	RAATTY	7 cut(s)	223, 312, 384, 1162, 1361, 1638, 1955
AcuI	CTGAAG	3 cut(s)	141, 1058, 2028
AfaI	GTAC	4 cut(s)	452, 519, 1044, 1794
AfiI	CCNNNNNNNGG	6 cut(s)	460, 540, 715, 855, 1705, 1853
AjnI	CCWGG	6 cut(s)	233, 495, 877, 1000, 1142, 1698
AjuI	GAANNNNNNNTTGG	2 cut(s)	1902, 1934
AloI	GAACNNNNNNTCC	3 cut(s)	33, 1896, 1928
AluBI	AGCT	8 cut(s)	119, 365, 566, 907, 974, 982, 1634, 2012
AluI	AGCT	8 cut(s)	119, 365, 566, 907, 974, 982, 1634, 2012
Alw21I	GWGCWC	1 cut(s)	1776
AlwI	GGATC	6 cut(s)	758, 923, 992, 1134, 1147, 1984
AlwNI	CAGNNNCTG	1 cut(s)	1193
Ama87I	CYCGRG	1 cut(s)	1548
AoxI	GGCC	3 cut(s)	134, 1351, 1928
ApeKI	GCWGC	5 cut(s)	56, 116, 1220, 1337, 1625
ApoI	RAATTY	7 cut(s)	223, 312, 384, 1162, 1361, 1638, 1955
ArsI	GACNNNNNNTTYG	2 cut(s)	1714, 1746
Asp718I	GGTACC	1 cut(s)	1042
AspLEI	GCGC	1 cut(s)	2068
AspS9I	GGNCC	5 cut(s)	34, 1229, 1238, 1402, 2003
AsuHPI	GGTGA	2 cut(s)	307, 1425
AsuII	TTCGAA	1 cut(s)	1583
AvaI	CYCGRG	1 cut(s)	1548
AvaII	GGWCC	5 cut(s)	34, 1229, 1238, 1402, 2003
AxyI	CCTNAGG	2 cut(s)	455, 1259
BaeI	ACNNNNGTAYC	2 cut(s)	434, 467
BamHI	GGATCC	1 cut(s)	1139
BanI	GGYRCC	1 cut(s)	1042
BbsI	GAAGAC	7 cut(s)	296, 334, 784, 865, 952, 1536, 1822
Bbv12I	GWGCWC	1 cut(s)	1776
BbvI	GCAGC	5 cut(s)	43, 128, 1232, 1349, 1612
BccI	CCATC	4 cut(s)	816, 1476, 1615, 1803
BceAI	ACGGC	2 cut(s)	518, 1613
BciT130I	CCWGG	6 cut(s)	235, 497, 879, 1002, 1144, 1700
BciVI	GTATCC	3 cut(s)	537, 1658, 1885
BclI	TGATCA	1 cut(s)	1195
BfaI	CTAG	5 cut(s)	321, 899, 927, 1095, 2110
BfmI	CTRYAG	1 cut(s)	975
BfuAI	ACCTGC	1 cut(s)	1249
BfuI	GTATCC	3 cut(s)	537, 1658, 1885
BglI	GCCNNNNNGGC	1 cut(s)	497
BglII	AGATCT	2 cut(s)	1741, 1944
BisI	GCNGC	6 cut(s)	57, 117, 1221, 1338, 1351, 1626
BlsI	GCNGC	6 cut(s)	58, 118, 1222, 1339, 1352, 1627
Bme1390I	CCNGG	6 cut(s)	235, 497, 879, 1002, 1144, 1700
Bme18I	GGWCC	5 cut(s)	34, 1229, 1238, 1402, 2003
BmeT110I	CYCGRG	1 cut(s)	1548
BmgT120I	GGNCC	5 cut(s)	34, 1229, 1238, 1402, 2003
BmiI	GGNNCC	3 cut(s)	240, 1044, 1141
BmrFI	CCNGG	6 cut(s)	235, 497, 879, 1002, 1144, 1700
BmrI	ACTGGG	1 cut(s)	705
BmsI	GCATC	6 cut(s)	1089, 1093, 1180, 1326, 1791, 2039
BmuI	ACTGGG	1 cut(s)	705
BpiI	GAAGAC	7 cut(s)	296, 334, 784, 865, 952, 1536, 1822
BpmI	CTGGAG	2 cut(s)	1308, 1821
Bpu14I	TTCGAA	1 cut(s)	1583
BpuEI	CTTGAG	5 cut(s)	14, 431, 992, 1479, 1919
BsaBI	GATNNNNATC	2 cut(s)	749, 1194
BsaJI	CCNNGG	5 cut(s)	233, 234, 622, 877, 1698
BsaXI	ACNNNNNCTCC	2 cut(s)	1980, 2010
Bsc4I	CCNNNNNNNGG	6 cut(s)	460, 540, 715, 855, 1705, 1853
Bse1I	ACTGG	5 cut(s)	700, 1089, 1454, 1603, 1838
Bse21I	CCTNAGG	2 cut(s)	455, 1259
Bse8I	GATNNNNATC	2 cut(s)	749, 1194
BseBI	CCWGG	6 cut(s)	235, 497, 879, 1002, 1144, 1700
BseDI	CCNNGG	5 cut(s)	233, 234, 622, 877, 1698
BseGI	GGATG	1 cut(s)	1626
BseJI	GATNNNNATC	2 cut(s)	749, 1194
BseLI	CCNNNNNNNGG	6 cut(s)	460, 540, 715, 855, 1705, 1853
BseMII	CTCAG	3 cut(s)	104, 1304, 2110
BseNI	ACTGG	5 cut(s)	700, 1089, 1454, 1603, 1838
BseRI	GAGGAG	1 cut(s)	274
BseX3I	CGGCCG	1 cut(s)	1351
BseXI	GCAGC	5 cut(s)	43, 128, 1232, 1349, 1612
BseYI	CCCAGC	1 cut(s)	713
Bsh1285I	CGRYCG	1 cut(s)	1354
BshFI	GGCC	3 cut(s)	136, 1353, 1930
BshNI	GGYRCC	1 cut(s)	1042
BsiEI	CGRYCG	1 cut(s)	1354
BsiHKAI	GWGCWC	1 cut(s)	1776
BsiHKCI	CYCGRG	1 cut(s)	1548
BsiSI	CCGG	2 cut(s)	20, 849
BslFI	GGGAC	1 cut(s)	607
BslI	CCNNNNNNNGG	6 cut(s)	460, 540, 715, 855, 1705, 1853
BsmFI	GGGAC	1 cut(s)	607
BsmI	GAATGC	2 cut(s)	552, 942
BsnI	GGCC	3 cut(s)	136, 1353, 1930
BsoBI	CYCGRG	1 cut(s)	1548
Bsp119I	TTCGAA	1 cut(s)	1583
Bsp1286I	GDGCHC	1 cut(s)	1776
Bsp143I	GATC	9 cut(s)	750, 853, 915, 997, 1139, 1195, 1741, 1944, 1976
Bsp19I	CCATGG	1 cut(s)	622
BspACI	CCGC	1 cut(s)	1350
BspANI	GGCC	3 cut(s)	136, 1353, 1930
BspCNI	CTCAG	3 cut(s)	103, 1305, 2109
BspLI	GGNNCC	3 cut(s)	240, 1044, 1141
BspMI	ACCTGC	1 cut(s)	1249
BspPI	GGATC	6 cut(s)	758, 923, 992, 1134, 1147, 1984
BspQI	GCTCTTC	2 cut(s)	362, 1494
BspT104I	TTCGAA	1 cut(s)	1583
BspT107I	GGYRCC	1 cut(s)	1042
BsrI	ACTGG	5 cut(s)	700, 1089, 1454, 1603, 1838
BssECI	CCNNGG	5 cut(s)	233, 234, 622, 877, 1698
BssMI	GATC	9 cut(s)	750, 853, 915, 997, 1139, 1195, 1741, 1944, 1976
BssNAI	GTATAC	1 cut(s)	1014
BssT1I	CCWWGG	1 cut(s)	622
Bst1107I	GTATAC	1 cut(s)	1014
Bst2UI	CCWGG	6 cut(s)	235, 497, 879, 1002, 1144, 1700
Bst4CI	ACNGT	6 cut(s)	302, 871, 924, 1271, 1519, 1561
Bst6I	CTCTTC	5 cut(s)	21, 353, 362, 1494, 1935
BstBI	TTCGAA	1 cut(s)	1583
BstC8I	GCNNGC	2 cut(s)	493, 2042
BstDEI	CTNAG	8 cut(s)	90, 361, 455, 521, 911, 1259, 1313, 2096
BstDSI	CCRYGG	1 cut(s)	622
BstF5I	GGATG	1 cut(s)	1626
BstHHI	GCGC	1 cut(s)	2068
BstKTI	GATC	9 cut(s)	753, 856, 918, 1000, 1142, 1198, 1744, 1947, 1979
BstMBI	GATC	9 cut(s)	750, 853, 915, 997, 1139, 1195, 1741, 1944, 1976
BstMCI	CGRYCG	1 cut(s)	1354
BstMWI	GCNNNNNNNGC	5 cut(s)	82, 238, 497, 1631, 2072
BstNI	CCWGG	6 cut(s)	235, 497, 879, 1002, 1144, 1700
BstSCI	CCNGG	6 cut(s)	233, 495, 877, 1000, 1142, 1698
BstSFI	CTRYAG	1 cut(s)	975
BstV1I	GCAGC	5 cut(s)	43, 128, 1232, 1349, 1612
BstV2I	GAAGAC	7 cut(s)	296, 334, 784, 865, 952, 1536, 1822
BstX2I	RGATCY	5 cut(s)	997, 1139, 1741, 1944, 1976
BstYI	RGATCY	5 cut(s)	997, 1139, 1741, 1944, 1976
BstZ17I	GTATAC	1 cut(s)	1014
BstZI	CGGCCG	1 cut(s)	1351
Bsu36I	CCTNAGG	2 cut(s)	455, 1259
BsuI	GTATCC	3 cut(s)	537, 1658, 1885
BsuRI	GGCC	3 cut(s)	136, 1353, 1930
BtgI	CCRYGG	1 cut(s)	622
BtgZI	GCGATG	1 cut(s)	1750
BtsCI	GGATG	1 cut(s)	1626
BtsI	GCAGTG	2 cut(s)	758, 1776
BtsIMutI	CAGTG	4 cut(s)	758, 1070, 1776, 2031
BveI	ACCTGC	1 cut(s)	1249
Cac8I	GCNNGC	2 cut(s)	493, 2042
CaiI	CAGNNNCTG	1 cut(s)	1193
CfoI	GCGC	1 cut(s)	2068
Cfr13I	GGNCC	5 cut(s)	34, 1229, 1238, 1402, 2003
Csp6I	GTAC	4 cut(s)	451, 518, 1043, 1793
CviAII	CATG	8 cut(s)	40, 352, 623, 817, 845, 1201, 1589, 1907
CviQI	GTAC	4 cut(s)	451, 518, 1043, 1793
DdeI	CTNAG	8 cut(s)	90, 361, 455, 521, 911, 1259, 1313, 2096
DpnI	GATC	9 cut(s)	752, 855, 917, 999, 1141, 1197, 1743, 1946, 1978
DpnII	GATC	9 cut(s)	750, 853, 915, 997, 1139, 1195, 1741, 1944, 1976
DraI	TTTAAA	1 cut(s)	2061
DrdI	GACNNNNNNGTC	1 cut(s)	1608
DseDI	GACNNNNNNGTC	1 cut(s)	1608
EaeI	YGGCCR	1 cut(s)	1351
EagI	CGGCCG	1 cut(s)	1351
Eam1104I	CTCTTC	5 cut(s)	21, 353, 362, 1494, 1935
EarI	CTCTTC	5 cut(s)	21, 353, 362, 1494, 1935
EclXI	CGGCCG	1 cut(s)	1351
Eco130I	CCWWGG	1 cut(s)	622
Eco147I	AGGCCT	2 cut(s)	136, 1930
Eco32I	GATATC	1 cut(s)	578
Eco47I	GGWCC	5 cut(s)	34, 1229, 1238, 1402, 2003
Eco52I	CGGCCG	1 cut(s)	1351
Eco57I	CTGAAG	3 cut(s)	141, 1058, 2028
Eco81I	CCTNAGG	2 cut(s)	455, 1259
Eco88I	CYCGRG	1 cut(s)	1548
EcoO109I	RGGNCCY	2 cut(s)	34, 1229
EcoRII	CCWGG	6 cut(s)	233, 495, 877, 1000, 1142, 1698
EcoRV	GATATC	1 cut(s)	578
EcoT14I	CCWWGG	1 cut(s)	622
EcoT22I	ATGCAT	1 cut(s)	1211
ErhI	CCWWGG	1 cut(s)	622
FaeI	CATG	8 cut(s)	43, 355, 626, 820, 848, 1204, 1592, 1910
FalI	AAGNNNNNCTT	2 cut(s)	124, 156
FaqI	GGGAC	1 cut(s)	607
FatI	CATG	8 cut(s)	39, 351, 622, 816, 844, 1200, 1588, 1906
FauNDI	CATATG	1 cut(s)	1754
FbaI	TGATCA	1 cut(s)	1195
FblI	GTMKAC	2 cut(s)	1013, 1273
Fnu4HI	GCNGC	6 cut(s)	57, 117, 1221, 1338, 1351, 1626
FokI	GGATG	1 cut(s)	1633
Fsp4HI	GCNGC	6 cut(s)	57, 117, 1221, 1338, 1351, 1626
FspBI	CTAG	5 cut(s)	321, 899, 927, 1095, 2110
GlaI	GCGC	1 cut(s)	2067
GluI	GCNGC	6 cut(s)	57, 117, 1221, 1338, 1351, 1626
GsaI	CCCAGC	1 cut(s)	717
GsuI	CTGGAG	2 cut(s)	1308, 1821
HaeIII	GGCC	3 cut(s)	136, 1353, 1930
HapII	CCGG	2 cut(s)	20, 849
HhaI	GCGC	1 cut(s)	2068
Hin1II	CATG	8 cut(s)	43, 355, 626, 820, 848, 1204, 1592, 1910
Hin6I	GCGC	1 cut(s)	2066
HinP1I	GCGC	1 cut(s)	2066
HindIII	AAGCTT	1 cut(s)	564
HpaII	CCGG	2 cut(s)	20, 849
HphI	GGTGA	2 cut(s)	307, 1425
Hpy166II	GTNNAC	6 cut(s)	298, 1014, 1274, 1564, 1613, 2003
Hpy188I	TCNGA	8 cut(s)	383, 543, 1038, 1135, 1189, 1314, 1473, 1981
Hpy188III	TCNNGA	9 cut(s)	400, 668, 927, 1123, 1159, 1396, 1496, 1548, 1618
Hpy8I	GTNNAC	6 cut(s)	298, 1014, 1274, 1564, 1613, 2003
HpyAV	CCTTC	7 cut(s)	1033, 1130, 1220, 1393, 1474, 1737, 1920
HpyCH4III	ACNGT	6 cut(s)	302, 871, 924, 1271, 1519, 1561
HpyCH4V	TGCA	9 cut(s)	942, 1209, 1220, 1337, 1555, 1592, 1782, 1952, 2105
HpyF10VI	GCNNNNNNNGC	5 cut(s)	82, 238, 497, 1631, 2072
HpyF3I	CTNAG	8 cut(s)	90, 361, 455, 521, 911, 1259, 1313, 2096
Hsp92II	CATG	8 cut(s)	43, 355, 626, 820, 848, 1204, 1592, 1910
HspAI	GCGC	1 cut(s)	2066
KpnI	GGTACC	1 cut(s)	1046
Ksp22I	TGATCA	1 cut(s)	1195
Kzo9I	GATC	9 cut(s)	750, 853, 915, 997, 1139, 1195, 1741, 1944, 1976
LguI	GCTCTTC	2 cut(s)	362, 1494
LmnI	GCTCC	1 cut(s)	238
Lsp1109I	GCAGC	5 cut(s)	43, 128, 1232, 1349, 1612
LweI	GCATC	6 cut(s)	1089, 1093, 1180, 1326, 1791, 2039
MaeI	CTAG	5 cut(s)	321, 899, 927, 1095, 2110
MaeIII	GTNAC	2 cut(s)	1489, 1513
MalI	GATC	9 cut(s)	752, 855, 917, 999, 1141, 1197, 1743, 1946, 1978
MboI	GATC	9 cut(s)	750, 853, 915, 997, 1139, 1195, 1741, 1944, 1976
MflI	RGATCY	5 cut(s)	997, 1139, 1741, 1944, 1976
MhlI	GDGCHC	1 cut(s)	1776
MlyI	GAGTC	7 cut(s)	966, 999, 1486, 1550, 1596, 1717, 1998
Mph1103I	ATGCAT	1 cut(s)	1211
MseI	TTAA	5 cut(s)	162, 407, 426, 1128, 2060
MslI	CAYNNNNRTG	3 cut(s)	1769, 2035, 2049
MspA1I	CMGCKG	1 cut(s)	119
MspI	CCGG	2 cut(s)	20, 849
MspR9I	CCNGG	6 cut(s)	235, 497, 879, 1002, 1144, 1700
Mva1269I	GAATGC	2 cut(s)	552, 942
MvaI	CCWGG	6 cut(s)	235, 497, 879, 1002, 1144, 1700
MwoI	GCNNNNNNNGC	5 cut(s)	82, 238, 497, 1631, 2072
NcoI	CCATGG	1 cut(s)	622
NdeI	CATATG	1 cut(s)	1754
NdeII	GATC	9 cut(s)	750, 853, 915, 997, 1139, 1195, 1741, 1944, 1976
NlaIII	CATG	8 cut(s)	43, 355, 626, 820, 848, 1204, 1592, 1910
NlaIV	GGNNCC	3 cut(s)	240, 1044, 1141
NmuCI	GTSAC	2 cut(s)	1489, 1513
NsiI	ATGCAT	1 cut(s)	1211
NspV	TTCGAA	1 cut(s)	1583
PaeR7I	CTCGAG	1 cut(s)	1548
PasI	CCCWGGG	1 cut(s)	234
PceI	AGGCCT	2 cut(s)	136, 1930
PciSI	GCTCTTC	2 cut(s)	362, 1494
PctI	GAATGC	2 cut(s)	552, 942
PfeI	GAWTC	3 cut(s)	433, 1585, 2021
PfoI	TCCNGGA	2 cut(s)	1000, 1142
PkrI	GCNGC	6 cut(s)	58, 118, 1222, 1339, 1352, 1627
PleI	GAGTC	7 cut(s)	965, 999, 1486, 1549, 1596, 1717, 1997
PpsI	GAGTC	7 cut(s)	965, 999, 1486, 1549, 1596, 1717, 1997
PpuMI	RGGWCCY	2 cut(s)	34, 1229
PsiI	TTATAA	1 cut(s)	1805
Psp5II	RGGWCCY	2 cut(s)	34, 1229
Psp6I	CCWGG	6 cut(s)	233, 495, 877, 1000, 1142, 1698
PspFI	CCCAGC	1 cut(s)	713
PspGI	CCWGG	6 cut(s)	233, 495, 877, 1000, 1142, 1698
PspN4I	GGNNCC	3 cut(s)	240, 1044, 1141
PspPI	GGNCC	5 cut(s)	34, 1229, 1238, 1402, 2003
PspPPI	RGGWCCY	2 cut(s)	34, 1229
PstNI	CAGNNNCTG	1 cut(s)	1193
PsuI	RGATCY	5 cut(s)	997, 1139, 1741, 1944, 1976
PvuII	CAGCTG	1 cut(s)	119
RsaI	GTAC	4 cut(s)	452, 519, 1044, 1794
RsaNI	GTAC	4 cut(s)	451, 518, 1043, 1793
RseI	CAYNNNNRTG	3 cut(s)	1769, 2035, 2049
SapI	GCTCTTC	2 cut(s)	362, 1494
SaqAI	TTAA	5 cut(s)	162, 407, 426, 1128, 2060
SatI	GCNGC	6 cut(s)	57, 117, 1221, 1338, 1351, 1626
Sau3AI	GATC	9 cut(s)	750, 853, 915, 997, 1139, 1195, 1741, 1944, 1976
Sau96I	GGNCC	5 cut(s)	34, 1229, 1238, 1402, 2003
SchI	GAGTC	7 cut(s)	966, 999, 1486, 1550, 1596, 1717, 1998
ScrFI	CCNGG	6 cut(s)	235, 497, 879, 1002, 1144, 1700
SduI	GDGCHC	1 cut(s)	1776
SfaNI	GCATC	6 cut(s)	1089, 1093, 1180, 1326, 1791, 2039
SfcI	CTRYAG	1 cut(s)	975
Sfr274I	CTCGAG	1 cut(s)	1548
SfuI	TTCGAA	1 cut(s)	1583
SinI	GGWCC	5 cut(s)	34, 1229, 1238, 1402, 2003
SlaI	CTCGAG	1 cut(s)	1548
SmiMI	CAYNNNNRTG	3 cut(s)	1769, 2035, 2049
SmlI	CTYRAG	6 cut(s)	29, 446, 1007, 1494, 1548, 1898
SmoI	CTYRAG	6 cut(s)	29, 446, 1007, 1494, 1548, 1898
SseBI	AGGCCT	2 cut(s)	136, 1930
SsiI	CCGC	1 cut(s)	1350
SspI	AATATT	1 cut(s)	885
SspMI	CTAG	5 cut(s)	321, 899, 927, 1095, 2110
StuI	AGGCCT	2 cut(s)	136, 1930
StyD4I	CCNGG	6 cut(s)	233, 495, 877, 1000, 1142, 1698
StyI	CCWWGG	1 cut(s)	622
TaaI	ACNGT	6 cut(s)	302, 871, 924, 1271, 1519, 1561
TaqI	TCGA	3 cut(s)	1549, 1583, 1605
TatI	WGTACW	1 cut(s)	517
TauI	GCSGC	1 cut(s)	1353
TfiI	GAWTC	3 cut(s)	433, 1585, 2021
Tru1I	TTAA	5 cut(s)	162, 407, 426, 1128, 2060
Tru9I	TTAA	5 cut(s)	162, 407, 426, 1128, 2060
TscAI	CASTG	4 cut(s)	765, 1070, 1776, 2031
TseFI	GTSAC	2 cut(s)	1489, 1513
TseI	GCWGC	5 cut(s)	56, 116, 1220, 1337, 1625
Tsp45I	GTSAC	2 cut(s)	1489, 1513
TspDTI	ATGAA	8 cut(s)	340, 631, 833, 1217, 1678, 1753, 1923, 2030
TspRI	CASTG	4 cut(s)	765, 1070, 1776, 2031
VpaK11BI	GGWCC	5 cut(s)	34, 1229, 1238, 1402, 2003
XapI	RAATTY	7 cut(s)	223, 312, 384, 1162, 1361, 1638, 1955
XbaI	TCTAGA	1 cut(s)	926
XcmI	CCANNNNNNNNNTGG	2 cut(s)	93, 2048
XhoI	CTCGAG	1 cut(s)	1548
XmiI	GTMKAC	2 cut(s)	1013, 1273
XspI	CTAG	5 cut(s)	321, 899, 927, 1095, 2110
Zsp2I	ATGCAT	1 cut(s)	1211

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.