Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0004752

Physical Location & Seq

Genomic Coordinates Reverse (-)

30651845 .. 30655938

Gene Structure 4094 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1166400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2121 bp

ATGGCAAAGTCCACTCTTCTGTTTGTTCTTAAGGCACTCATGGCTTTGATATTTGCAGCTTGGATTTCTCTTTGGCTTCTGAAACCAACACAGCTATGGACTAAAAAATGGAAGGGAGCTGAAGATGCAGCAAGAAATACAGTGTTTGGATATTATGGTCTAAATTTTGCTGTGTATACATTCCCTCCTATTGCTTTGGCAATTATTGGATCTCTCTACTTGAATTGGAAGCCCAAGGAGCCAATAAGGAGAAAAGGGAGGGGATTGATTGCTGCAGGTTTCTCAAATCCATTGGTGGTGAATGCTCTTGTTGGTGTTTTATCCACTCTAGAAATTCTAGCAGTGTTCCTCTTCCTCCTCTTCTTAGCTTGGAATTTTTACGCTCGAATCTCTAATGATTTCAAGAACTTGAAGCCCTCAAAATCGATAGTGCTGAATTCATGGCAACTCAAGTACTTAAGGGTGGCAACTCGGTTCGGTTTGCTAGCAGAAGCCTGTCTGGCTTTTCTTCTCTTCCCTATCTTAAGAGGGCTGGCCTTATTCCGGGTTCTCGGCATCCAGTTTGAAGCTTCAATCAAATATCATACATGGCTTGGCACTGCAATGCTATTCTTTGCCACTTTCCATGGTGCAAGCACTTTGTTCATTTGGGGTGTCGGCCACTATATCCAAGAGGAGATATGGAAATGGCAGAACACCGGGCGGATATACATTGCTGGGGAGATCTGTCTAGTTGTAGGGTTAGTAATTTGGGTCACATCACTTCCTCAAATAAGGAGAAAGAAGTTCGAGATATTTTACTACACACACCATCTCTATATGGTTTTCCTAGTGTTCTTCTTGTTTCATGCTGGAGATCGTCACTTCTACACAGTTTTCCCTGGAATTTTTCTGTTTGGCCTTGACAAGCTGCTTCGAATCATACAATCCAGACCCGAAACTTGTATCCTTTCTGCCCAAATTTTCCCCTCCAGCGCTATCGAGCTTATTCTACCAAAAGATCCAGGACTCAAGTATACACCAACAAGTGTTATATTTGTGAAGGTACCAAGCATATCCAAATATCAGTGGCACTCATTCAGCATTATCTCCAGCTCTAGTGTCGATAAGAACACAATGTCGATTTTAATCAAATCTGAAGGATCGTGGACTAGTTCTCTCTCAAATATGATCCGAACTAGGCAAGAGTCGGAAAACGATCAGCTGAAGTACATACCTATAGCAGTAGAAGGCCCTTATGGACCTGTCTCTATGGACTTCCTAAGATATGACAGCCTAATGCTTGTTGCTGGAGGAATTGGGATAACCCCATTTCTGAGCATCTTACAGGAGCTTGCTTCGTTTGAAAACAACAGCACACACAAATTCCTGCCAAGAATACAACTTATATATGCTGTGAAGAAGTCAGAAGACATTTGTCTATTGAACTCGATCTCAACCCTAATCTTTAACCAGCCATCTGAAAAATGCCGTCTGAAAGTTAAAATATTTGTGACACAAGAGCAGTATGGTACAACAGTAAGAGAACTACTCAACGAGTTTTCACAAGTGAAAACGGTACACTTTGGTACAAAGTTCTTGAATTATTCGATCAATGGACTCGAAAGCCCAGTTTGGATGGCTGCCATAACTGGAATCTCTTCCATCCTGTTTCTGATCTTTCTTATCATTTTCAACCACATTTTTATTCCCTCACAAAAAATGGCCTCCGAAGCAACGAAAGAAAAGACTCCGTCTTCGATTGCAGATCTGCTTATCATATCTTCTTTCATTGTAGCAATAACATGCAGCATTTTTGTTGCACTTATTGTGAGACGGAGAAGGATACAGAAGTTGATTCCTCCAATCCTCCAAAAACAAGGGAAAGACTTGGAAAAAAGCTCAATACAAGCAAGTGATGCTGCTCTTGAGGAACATGAAATCCATTTTGGAGGCAGGCCTAAATTTCAAGATATCTTTGCAAAGTTTTCAGATGAGACGGGTGGATCTGATATCGGAGTGATGGTGTGTGGACCGGAGAGTATGAAAGAATCAGTGGCAACAGAATGCCAGCAGAAGTTGGGTGCTAATAAGAAGCAGAAGGCCAGTTTCAGCTTTCACTCCCTCAATTTCACACTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000293 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005886 GO:0008150 GO:0008152 GO:0016020 GO:0016491 GO:0016722 GO:0016723 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_8 Ferric_reduct NAD_binding_6

Protein Analysis

707

Amino Acids

79.84

Weight (kDa)

9.52

Isoelectric Point (pI)

39.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ferric_reduct	PF01794	159 - 280	1.6e-20	Ferric reductase like transmembrane component
FAD_binding_8	PF08022	317 - 416	9.4e-20	FAD-binding domain
NAD_binding_6	PF08030	423 - 683	5.2e-21	Ferric reductase NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004752)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G50160 AT5G50160
fragaria_vesca	FvH4_5g06130 FvH4_5g06130 FvH4_5g06130
malus_domestica	MD06G1166400.v1.1
prunus_persica	Prupe.5G166400_v2.0.a1 Prupe.5G166400_v2.0.a1 Prupe.5G166400_v2.0.a1
pyrus_communis	pycom06g14910
rosa_chinensis	RchiOBHm_Chr7g0192661 RchiOBHm_Chr7g0192671 RchiOBHm_Chr7g0192681
rosa_laevigata	RLG00000004337 RLG00000004363 RLG00000004456
rosa_multiflora	Rmu_sc0002040.1_g000005
rosa_roxburghii	Rroxscaffold_3G00262750
rosa_rugosa	Rorug07G0003700 Rorug07G0003800
rosa_samantha	Rh7AG128600 Rh7AG128800 Rh7BG130200 Rh7BG130300 Rh7CG133400 Rh7CG133500 Rh7CG133600 Rh7CG133700 Rh7DG131100
rosa_wichuraiana	Rw7G011000 Rw7G011020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	266
Acc65I	GGTACC	1 cut(s)	1045
AccB1I	GGYRCC	1 cut(s)	1045
AccI	GTMKAC	2 cut(s)	176, 1016
AciI	CCGC	1 cut(s)	703
AclWI	GGATC	5 cut(s)	217, 995, 1150, 1165, 1993
AcoI	YGGCCR	1 cut(s)	658
AcsI	RAATTY	8 cut(s)	163, 333, 373, 436, 885, 960, 1364, 1943
AcuI	CTGAAG	3 cut(s)	141, 1158, 1226
AfaI	GTAC	6 cut(s)	455, 1047, 1211, 1513, 1560, 1570
AfeI	AGCGCT	1 cut(s)	976
AfiI	CCNNNNNNNGG	1 cut(s)	543
AflII	CTTAAG	3 cut(s)	29, 457, 523
AhlI	ACTAGT	1 cut(s)	1151
AjnI	CCWGG	2 cut(s)	880, 1003
AjuI	GAANNNNNNNTTGG	2 cut(s)	1911, 1943
AloI	GAACNNNNNNTCC	2 cut(s)	1905, 1937
Alw26I	GTCTC	3 cut(s)	1252, 1807, 1970
AlwI	GGATC	5 cut(s)	217, 995, 1150, 1165, 1993
Aor51HI	AGCGCT	1 cut(s)	976
AoxI	GGCC	7 cut(s)	534, 658, 898, 1231, 1704, 1937, 2082
ApeKI	GCWGC	7 cut(s)	56, 128, 272, 910, 1622, 1788, 1901
ApoI	RAATTY	8 cut(s)	163, 333, 373, 436, 885, 960, 1364, 1943
ArsI	GACNNNNNNTTYG	2 cut(s)	1720, 1752
Asp700I	GAANNNNTTC	1 cut(s)	1639
Asp718I	GGTACC	1 cut(s)	1045
AspLEI	GCGC	1 cut(s)	977
AspS9I	GGNCC	3 cut(s)	1232, 1241, 2012
AsuC2I	CCSGG	2 cut(s)	545, 700
AsuHPI	GGTGA	1 cut(s)	310
AsuII	TTCGAA	1 cut(s)	916
AsuNHI	GCTAGC	1 cut(s)	484
AvaII	GGWCC	2 cut(s)	1241, 2012
BaeI	ACNNNNGTAYC	4 cut(s)	1503, 1536, 1817, 1850
BanI	GGYRCC	1 cut(s)	1045
BbsI	GAAGAC	2 cut(s)	1416, 1728
BbvI	GCAGC	7 cut(s)	68, 140, 259, 897, 1609, 1800, 1888
BccI	CCATC	5 cut(s)	819, 1465, 1612, 1652, 1996
BceAI	ACGGC	1 cut(s)	1455
BciT130I	CCWGG	2 cut(s)	882, 1005
BciVI	GTATCC	2 cut(s)	956, 1818
BcnI	CCSGG	2 cut(s)	545, 700
BcoDI	GTCTC	3 cut(s)	1252, 1807, 1970
BcuI	ACTAGT	1 cut(s)	1151
BfaI	CTAG	8 cut(s)	329, 338, 485, 731, 830, 1098, 1152, 1179
BfmI	CTRYAG	2 cut(s)	273, 1218
BfoI	RGCGCY	1 cut(s)	978
BfrI	CTTAAG	3 cut(s)	29, 457, 523
BfuAI	ACCTGC	1 cut(s)	266
BfuI	GTATCC	2 cut(s)	956, 1818
BglI	GCCNNNNNGGC	1 cut(s)	500
BglII	AGATCT	2 cut(s)	723, 1747
BisI	GCNGC	7 cut(s)	57, 129, 273, 911, 1623, 1789, 1902
BlsI	GCNGC	7 cut(s)	58, 130, 274, 912, 1624, 1790, 1903
BmcAI	AGTACT	1 cut(s)	455
Bme1390I	CCNGG	4 cut(s)	545, 700, 882, 1005
Bme18I	GGWCC	2 cut(s)	1241, 2012
BmgT120I	GGNCC	3 cut(s)	1232, 1241, 2012
BmiI	GGNNCC	2 cut(s)	240, 1047
BmrFI	CCNGG	4 cut(s)	545, 700, 882, 1005
BmrI	ACTGGG	1 cut(s)	1604
BmsI	GCATC	4 cut(s)	115, 564, 1329, 1888
BmtI	GCTAGC	1 cut(s)	488
BmuI	ACTGGG	1 cut(s)	1604
BoxI	GACNNNNGTC	1 cut(s)	1416
BpiI	GAAGAC	2 cut(s)	1416, 1728
BplI	GAGNNNNNCTC	2 cut(s)	1515, 1547
BpmI	CTGGAG	4 cut(s)	873, 955, 1075, 1311
Bpu14I	TTCGAA	1 cut(s)	916
BpuEI	CTTGAG	3 cut(s)	434, 995, 1928
BpuMI	CCSGG	2 cut(s)	545, 700
Bsa29I	ATCGAT	1 cut(s)	425
BsaBI	GATNNNNATC	1 cut(s)	1127
BsaJI	CCNNGG	3 cut(s)	234, 625, 880
BsaWI	WCCGGW	1 cut(s)	2014
BsaXI	ACNNNNNCTCC	4 cut(s)	169, 199, 1989, 2019
Bsc4I	CCNNNNNNNGG	1 cut(s)	543
Bse1I	ACTGG	4 cut(s)	559, 1610, 1636, 2085
Bse3DI	GCAATG	2 cut(s)	609, 711
Bse8I	GATNNNNATC	1 cut(s)	1127
BseBI	CCWGG	2 cut(s)	882, 1005
BseCI	ATCGAT	1 cut(s)	425
BseDI	CCNNGG	3 cut(s)	234, 625, 880
BseGI	GGATG	3 cut(s)	555, 1623, 1644
BseJI	GATNNNNATC	1 cut(s)	1127
BseLI	CCNNNNNNNGG	1 cut(s)	543
BseMI	GCAATG	2 cut(s)	609, 711
BseMII	CTCAG	1 cut(s)	1307
BseNI	ACTGG	4 cut(s)	559, 1610, 1636, 2085
BseRI	GAGGAG	2 cut(s)	347, 689
BseXI	GCAGC	7 cut(s)	68, 140, 259, 897, 1609, 1800, 1888
BseYI	CCCAGC	1 cut(s)	716
BshFI	GGCC	7 cut(s)	536, 660, 900, 1233, 1706, 1939, 2084
BshNI	GGYRCC	1 cut(s)	1045
BshVI	ATCGAT	1 cut(s)	425
BsiSI	CCGG	3 cut(s)	544, 699, 2015
BslI	CCNNNNNNNGG	1 cut(s)	543
BsmAI	GTCTC	3 cut(s)	1252, 1807, 1970
BsmBI	CGTCTC	2 cut(s)	1807, 1970
BsmI	GAATGC	2 cut(s)	307, 2051
BsnI	GGCC	7 cut(s)	536, 660, 900, 1233, 1706, 1939, 2084
Bsp119I	TTCGAA	1 cut(s)	916
Bsp19I	CCATGG	1 cut(s)	625
BspACI	CCGC	1 cut(s)	703
BspANI	GGCC	7 cut(s)	536, 660, 900, 1233, 1706, 1939, 2084
BspCNI	CTCAG	1 cut(s)	1308
BspDI	ATCGAT	1 cut(s)	425
BspLI	GGNNCC	2 cut(s)	240, 1047
BspMAI	CTGCAG	1 cut(s)	277
BspMI	ACCTGC	1 cut(s)	266
BspOI	GCTAGC	1 cut(s)	488
BspPI	GGATC	5 cut(s)	217, 995, 1150, 1165, 1993
BspT104I	TTCGAA	1 cut(s)	916
BspT107I	GGYRCC	1 cut(s)	1045
BspTI	CTTAAG	3 cut(s)	29, 457, 523
BsrDI	GCAATG	2 cut(s)	609, 711
BsrI	ACTGG	4 cut(s)	559, 1610, 1636, 2085
BssECI	CCNNGG	3 cut(s)	234, 625, 880
BssNAI	GTATAC	2 cut(s)	177, 1017
BssT1I	CCWWGG	2 cut(s)	234, 625
Bst1107I	GTATAC	2 cut(s)	177, 1017
Bst2UI	CCWGG	2 cut(s)	882, 1005
Bst4CI	ACNGT	4 cut(s)	142, 874, 1519, 1558
Bst6I	CTCTTC	5 cut(s)	21, 356, 365, 518, 1645
BstAFI	CTTAAG	3 cut(s)	29, 457, 523
BstAPI	GCANNNNNTGC	1 cut(s)	1898
BstBI	TTCGAA	1 cut(s)	916
BstC8I	GCNNGC	6 cut(s)	486, 534, 634, 1335, 1937, 2051
BstDEI	CTNAG	3 cut(s)	364, 1262, 1316
BstDSI	CCRYGG	1 cut(s)	625
BstF5I	GGATG	3 cut(s)	555, 1623, 1644
BstH2I	RGCGCY	1 cut(s)	978
BstHHI	GCGC	1 cut(s)	977
BstMAI	GTCTC	3 cut(s)	1252, 1807, 1970
BstMWI	GCNNNNNNNGC	6 cut(s)	41, 125, 238, 500, 1712, 1898
BstNI	CCWGG	2 cut(s)	882, 1005
BstNSI	RCATGY	1 cut(s)	1788
BstPAI	GACNNNNGTC	1 cut(s)	1416
BstSCI	CCNGG	4 cut(s)	543, 698, 880, 1003
BstSFI	CTRYAG	2 cut(s)	273, 1218
BstV1I	GCAGC	7 cut(s)	68, 140, 259, 897, 1609, 1800, 1888
BstV2I	GAAGAC	2 cut(s)	1416, 1728
BstX2I	RGATCY	5 cut(s)	209, 723, 1000, 1747, 1985
BstYI	RGATCY	5 cut(s)	209, 723, 1000, 1747, 1985
BstZ17I	GTATAC	2 cut(s)	177, 1017
Bsu15I	ATCGAT	1 cut(s)	425
BsuI	GTATCC	2 cut(s)	956, 1818
BsuRI	GGCC	7 cut(s)	536, 660, 900, 1233, 1706, 1939, 2084
BsuTUI	ATCGAT	1 cut(s)	425
BtgI	CCRYGG	1 cut(s)	625
BtsCI	GGATG	3 cut(s)	555, 1623, 1644
BtsI	GCAGTG	2 cut(s)	348, 597
BtsIMutI	CAGTG	5 cut(s)	147, 348, 597, 1073, 2040
BveI	ACCTGC	1 cut(s)	266
Cac8I	GCNNGC	6 cut(s)	486, 534, 634, 1335, 1937, 2051
CfoI	GCGC	1 cut(s)	977
Cfr13I	GGNCC	3 cut(s)	1232, 1241, 2012
ClaI	ATCGAT	1 cut(s)	425
Csp6I	GTAC	6 cut(s)	454, 1046, 1210, 1512, 1559, 1569
CviAII	CATG	7 cut(s)	40, 441, 588, 626, 848, 1785, 1916
CviQI	GTAC	6 cut(s)	454, 1046, 1210, 1512, 1559, 1569
DdeI	CTNAG	3 cut(s)	364, 1262, 1316
EaeI	YGGCCR	1 cut(s)	658
Eam1104I	CTCTTC	5 cut(s)	21, 356, 365, 518, 1645
EarI	CTCTTC	5 cut(s)	21, 356, 365, 518, 1645
EciI	GGCGGA	1 cut(s)	718
Eco130I	CCWWGG	2 cut(s)	234, 625
Eco147I	AGGCCT	1 cut(s)	1939
Eco32I	GATATC	2 cut(s)	1954, 1993
Eco47I	GGWCC	2 cut(s)	1241, 2012
Eco47III	AGCGCT	1 cut(s)	976
Eco57I	CTGAAG	3 cut(s)	141, 1158, 1226
EcoO109I	RGGNCCY	1 cut(s)	1232
EcoRI	GAATTC	1 cut(s)	436
EcoRII	CCWGG	2 cut(s)	880, 1003
EcoRV	GATATC	2 cut(s)	1954, 1993
EcoT14I	CCWWGG	2 cut(s)	234, 625
ErhI	CCWWGG	2 cut(s)	234, 625
Esp3I	CGTCTC	2 cut(s)	1807, 1970
FaeI	CATG	7 cut(s)	43, 444, 591, 629, 851, 1788, 1919
FatI	CATG	7 cut(s)	39, 440, 587, 625, 847, 1784, 1915
FblI	GTMKAC	2 cut(s)	176, 1016
Fnu4HI	GCNGC	7 cut(s)	57, 129, 273, 911, 1623, 1789, 1902
FokI	GGATG	3 cut(s)	542, 1630, 1631
Fsp4HI	GCNGC	7 cut(s)	57, 129, 273, 911, 1623, 1789, 1902
FspBI	CTAG	8 cut(s)	329, 338, 485, 731, 830, 1098, 1152, 1179
GlaI	GCGC	1 cut(s)	976
GluI	GCNGC	7 cut(s)	57, 129, 273, 911, 1623, 1789, 1902
GsaI	CCCAGC	1 cut(s)	720
GsuI	CTGGAG	4 cut(s)	873, 955, 1075, 1311
HaeII	RGCGCY	1 cut(s)	978
HaeIII	GGCC	7 cut(s)	536, 660, 900, 1233, 1706, 1939, 2084
HapII	CCGG	3 cut(s)	544, 699, 2015
HhaI	GCGC	1 cut(s)	977
Hin1II	CATG	7 cut(s)	43, 444, 591, 629, 851, 1788, 1919
Hin6I	GCGC	1 cut(s)	975
HinP1I	GCGC	1 cut(s)	975
HindIII	AAGCTT	1 cut(s)	567
HinfI	GANTC	9 cut(s)	387, 918, 1008, 1187, 1599, 1635, 1729, 1837, 2030
HpaII	CCGG	3 cut(s)	544, 699, 2015
HphI	GGTGA	1 cut(s)	310
Hpy166II	GTNNAC	6 cut(s)	12, 177, 1017, 1149, 1561, 2012
Hpy188III	TCNNGA	7 cut(s)	329, 403, 790, 930, 1579, 1907, 1949
Hpy8I	GTNNAC	6 cut(s)	12, 177, 1017, 1149, 1561, 2012
HpyAV	CCTTC	6 cut(s)	106, 1036, 1133, 1223, 1815, 2074
HpyCH4III	ACNGT	4 cut(s)	142, 874, 1519, 1558
HpyCH4V	TGCA	9 cut(s)	56, 128, 275, 602, 632, 1745, 1788, 1802, 1961
HpyF10VI	GCNNNNNNNGC	6 cut(s)	41, 125, 238, 500, 1712, 1898
HpyF3I	CTNAG	3 cut(s)	364, 1262, 1316
Hsp92II	CATG	7 cut(s)	43, 444, 591, 629, 851, 1788, 1919
HspAI	GCGC	1 cut(s)	975
KpnI	GGTACC	1 cut(s)	1049
LmnI	GCTCC	3 cut(s)	116, 238, 1330
Lsp1109I	GCAGC	7 cut(s)	68, 140, 259, 897, 1609, 1800, 1888
LweI	GCATC	4 cut(s)	115, 564, 1329, 1888
MaeI	CTAG	8 cut(s)	329, 338, 485, 731, 830, 1098, 1152, 1179
MaeIII	GTNAC	3 cut(s)	754, 860, 1492
MflI	RGATCY	5 cut(s)	209, 723, 1000, 1747, 1985
MlyI	GAGTC	4 cut(s)	1002, 1196, 1593, 1723
MmeI	TCCRAC	1 cut(s)	1170
MroXI	GAANNNNTTC	1 cut(s)	1639
MseI	TTAA	6 cut(s)	30, 458, 524, 1127, 1449, 1482
MslI	CAYNNNNRTG	2 cut(s)	94, 602
MspA1I	CMGCKG	1 cut(s)	1204
MspCI	CTTAAG	3 cut(s)	29, 457, 523
MspI	CCGG	3 cut(s)	544, 699, 2015
MspR9I	CCNGG	4 cut(s)	545, 700, 882, 1005
Mva1269I	GAATGC	2 cut(s)	307, 2051
MvaI	CCWGG	2 cut(s)	882, 1005
MwoI	GCNNNNNNNGC	6 cut(s)	41, 125, 238, 500, 1712, 1898
NciI	CCSGG	2 cut(s)	545, 700
NcoI	CCATGG	1 cut(s)	625
NheI	GCTAGC	1 cut(s)	484
NlaIII	CATG	7 cut(s)	43, 444, 591, 629, 851, 1788, 1919
NlaIV	GGNNCC	2 cut(s)	240, 1047
NmeAIII	GCCGAG	1 cut(s)	531
NmuCI	GTSAC	3 cut(s)	754, 860, 1492
NspI	RCATGY	1 cut(s)	1788
NspV	TTCGAA	1 cut(s)	916
PceI	AGGCCT	1 cut(s)	1939
PctI	GAATGC	2 cut(s)	307, 2051
PdmI	GAANNNNTTC	1 cut(s)	1639
PfeI	GAWTC	5 cut(s)	387, 918, 1635, 1837, 2030
PflFI	GACNNNGTC	1 cut(s)	1732
PfoI	TCCNGGA	1 cut(s)	1003
PkrI	GCNGC	7 cut(s)	58, 130, 274, 912, 1624, 1790, 1903
PleI	GAGTC	4 cut(s)	1002, 1195, 1593, 1723
PpsI	GAGTC	4 cut(s)	1002, 1195, 1593, 1723
PshAI	GACNNNNGTC	1 cut(s)	1416
Psp6I	CCWGG	2 cut(s)	880, 1003
PspFI	CCCAGC	1 cut(s)	716
PspGI	CCWGG	2 cut(s)	880, 1003
PspN4I	GGNNCC	2 cut(s)	240, 1047
PspPI	GGNCC	3 cut(s)	1232, 1241, 2012
PstI	CTGCAG	1 cut(s)	277
PsuI	RGATCY	5 cut(s)	209, 723, 1000, 1747, 1985
PsyI	GACNNNGTC	1 cut(s)	1732
PvuII	CAGCTG	1 cut(s)	1204
RsaI	GTAC	6 cut(s)	455, 1047, 1211, 1513, 1560, 1570
RsaNI	GTAC	6 cut(s)	454, 1046, 1210, 1512, 1559, 1569
RseI	CAYNNNNRTG	2 cut(s)	94, 602
SaqAI	TTAA	6 cut(s)	30, 458, 524, 1127, 1449, 1482
SatI	GCNGC	7 cut(s)	57, 129, 273, 911, 1623, 1789, 1902
Sau96I	GGNCC	3 cut(s)	1232, 1241, 2012
ScaI	AGTACT	1 cut(s)	455
SchI	GAGTC	4 cut(s)	1002, 1196, 1593, 1723
ScrFI	CCNGG	4 cut(s)	545, 700, 882, 1005
SfaNI	GCATC	4 cut(s)	115, 564, 1329, 1888
SfcI	CTRYAG	2 cut(s)	273, 1218
SfuI	TTCGAA	1 cut(s)	916
SinI	GGWCC	2 cut(s)	1241, 2012
SmiMI	CAYNNNNRTG	2 cut(s)	94, 602
SmlI	CTYRAG	6 cut(s)	29, 449, 457, 523, 1010, 1907
SmoI	CTYRAG	6 cut(s)	29, 449, 457, 523, 1010, 1907
SpeI	ACTAGT	1 cut(s)	1151
SseBI	AGGCCT	1 cut(s)	1939
SsiI	CCGC	1 cut(s)	703
SspI	AATATT	1 cut(s)	1488
SspMI	CTAG	8 cut(s)	329, 338, 485, 731, 830, 1098, 1152, 1179
StuI	AGGCCT	1 cut(s)	1939
StyD4I	CCNGG	4 cut(s)	543, 698, 880, 1003
StyI	CCWWGG	2 cut(s)	234, 625
TaaI	ACNGT	4 cut(s)	142, 874, 1519, 1558
TatI	WGTACW	2 cut(s)	453, 1209
TfiI	GAWTC	5 cut(s)	387, 918, 1635, 1837, 2030
Tru1I	TTAA	6 cut(s)	30, 458, 524, 1127, 1449, 1482
Tru9I	TTAA	6 cut(s)	30, 458, 524, 1127, 1449, 1482
TscAI	CASTG	5 cut(s)	147, 348, 604, 1073, 2040
TseFI	GTSAC	3 cut(s)	754, 860, 1492
TseI	GCWGC	7 cut(s)	56, 128, 272, 910, 1622, 1788, 1901
Tsp45I	GTSAC	3 cut(s)	754, 860, 1492
TspDTI	ATGAA	6 cut(s)	429, 634, 836, 1759, 1932, 2039
TspGWI	ACGGA	2 cut(s)	1722, 1831
TspRI	CASTG	5 cut(s)	147, 348, 604, 1073, 2040
Tth111I	GACNNNGTC	1 cut(s)	1732
Vha464I	CTTAAG	3 cut(s)	29, 457, 523
VpaK11BI	GGWCC	2 cut(s)	1241, 2012
XapI	RAATTY	8 cut(s)	163, 333, 373, 436, 885, 960, 1364, 1943
XbaI	TCTAGA	1 cut(s)	328
XceI	RCATGY	1 cut(s)	1788
XcmI	CCANNNNNNNNNTGG	1 cut(s)	93
XmiI	GTMKAC	2 cut(s)	176, 1016
XmnI	GAANNNNTTC	1 cut(s)	1639
XspI	CTAG	8 cut(s)	329, 338, 485, 731, 830, 1098, 1152, 1179
ZrmI	AGTACT	1 cut(s)	455

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.