FvH4_5g08380

Polysaccharide biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014552
Physical Location & Seq
Reverse (-)
4868358 .. 4869323
966 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g08380.t1

Sequence Viewer

Length: 966 bp
ATGCCCCCAGAAGTGCCTCATTATCGATCTTCTGTAACTCCTCTAGTACGGTTCTCTCCTTCTGTCTCACCTGAAGCACAACTACATCAACCGTATAAATACAAGTACTGGAGAGGAGAGACCAACATGAACTTTGCCGGAAAGAAGCTCATCCCTTTTCTTGTTTTGCTCCTTTCGATCATTTCTGTACTCAGACTTGTTAGAATATCGATAACTACATCTTCTTCTTCTACTTCTTCACATCCACTAACTGCTTTAGCACCTCTTGGAGACTCATGTTCCAATCCTTCTTCATGCACTAAATTTCAACCAGAGAAAAGTTCCTCCGTTAACGCCACGGCCCTGCAGAAAAAGGAATACCAACTTCTGTCAAATCTCATTTCCCAGAGAGCCCCTTGTAACCTCCTCATTTTTGGGCTGGAGCCTCAGTATCTATCTCTCTCATCTATCAATGCAGGCGGCACTACCATCTTTCTGGAGGACGACGCTACCAAGATAAGCGCTATGACATCAGATTCCAACACCACCAGAATCTACAAGGTGGACTATAAGGTACCTGCAAAAGAGGCTTATAACCTGCTGAGGCATGCAAGGGAAACCCCAGTGTGTGCACCGAGCTCAAAACAGCTTCAAGTATCAGAATGTCGGCTTGCGTTGAAAAATTTGCCACAAGAAGTCTATGAGCTTAAATGGGATGTGGTGGTGGTGGATGGACCAAGCGGGAATAAGCCAGAGGCACCAGGCAGGATGGCAGCCATCTACACTGCAAGTATGTTAGCAAGAAATGGGAATATTACGGATGTACTAGTGCATGATGTGGATCGAACAATTGAGAAGTGGTTTTCCTGGGAGTTTCTGTGTGATGAGAACTTAGTTTCTTCAAAAGGGAAATTATGGAACTTTAGGATCAGGGGTCATACAAACTCTTCAAAATTTTGCCTTGCCGAAGCAGTTACAATAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polysacc_synt_4

Protein Analysis

322

Amino Acids

35.65

Weight (kDa)

8.97

Isoelectric Point (pI)

45.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 119 - 301 1.2e-69 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014552)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24910
fragaria_vesca FvH4_5g08380
malus_domestica MD06G1141300.v1.1 MD14G1156500.v1.1
prunus_persica Prupe.5G150600_v2.0.a1
pyrus_communis pycom06g13130 pycom14g13010
rosa_chinensis RchiOBHm_Chr7g0190071
rosa_laevigata RLG00000004567
rosa_multiflora Rmu_sc0004886.1_g000008
rosa_roxburghii Rroxscaffold_3G00264910
rosa_rugosa Rorug06G0502200
rosa_samantha Rh7AG107800 Rh7BG110100 Rh7CG112300 Rh7DG110700
rosa_wichuraiana Rw7G009300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 573
Acc36I ACCTGC 2 cut(s) 565, 585
Acc65I GGTACC 1 cut(s) 553
AccB1I GGYRCC 2 cut(s) 553, 736
AciI CCGC 2 cut(s) 459, 720
AclWI GGATC 2 cut(s) 828, 914
AcsI RAATTY 3 cut(s) 302, 661, 932
AcuI CTGAAG 1 cut(s) 93
AfaI GTAC 5 cut(s) 48, 107, 189, 555, 804
AfeI AGCGCT 1 cut(s) 502
AgsI TTSAA 5 cut(s) 308, 632, 658, 882, 930
AhlI ACTAGT 1 cut(s) 805
AjnI CCWGG 2 cut(s) 739, 845
AluBI AGCT 4 cut(s) 148, 618, 628, 685
AluI AGCT 4 cut(s) 148, 618, 628, 685
Alw21I GWGCWC 2 cut(s) 613, 620
Alw26I GTCTC 3 cut(s) 70, 113, 264
Alw44I GTGCAC 1 cut(s) 609
AlwI GGATC 2 cut(s) 828, 914
Aor51HI AGCGCT 1 cut(s) 502
AoxI GGCC 1 cut(s) 339
ApaLI GTGCAC 1 cut(s) 609
ApeKI GCWGC 1 cut(s) 752
ApoI RAATTY 3 cut(s) 302, 661, 932
Asp718I GGTACC 1 cut(s) 553
AspLEI GCGC 1 cut(s) 503
AspS9I GGNCC 2 cut(s) 340, 713
AsuHPI GGTGA 1 cut(s) 60
AvaII GGWCC 1 cut(s) 713
BaeGI GKGCMC 1 cut(s) 613
BanI GGYRCC 2 cut(s) 553, 736
BanII GRGCYC 2 cut(s) 394, 620
BarI GAAGNNNNNNTAC 2 cut(s) 66, 98
Bbv12I GWGCWC 2 cut(s) 613, 620
BbvCI CCTCAGC 1 cut(s) 581
BbvI GCAGC 1 cut(s) 764
BccI CCATC 4 cut(s) 476, 704, 742, 764
BceAI ACGGC 1 cut(s) 354
BciT130I CCWGG 2 cut(s) 741, 847
BcoDI GTCTC 3 cut(s) 70, 113, 264
BcuI ACTAGT 1 cut(s) 805
BfaI CTAG 2 cut(s) 44, 806
BfmI CTRYAG 1 cut(s) 344
BfoI RGCGCY 1 cut(s) 504
BfuAI ACCTGC 2 cut(s) 565, 585
BisI GCNGC 2 cut(s) 460, 753
BlsI GCNGC 2 cut(s) 461, 754
BmcAI AGTACT 1 cut(s) 107
Bme1390I CCNGG 2 cut(s) 741, 847
Bme18I GGWCC 1 cut(s) 713
BmgT120I GGNCC 2 cut(s) 340, 713
BmiI GGNNCC 3 cut(s) 423, 555, 738
BmrFI CCNGG 2 cut(s) 741, 847
BmrI ACTGGG 1 cut(s) 596
BmuI ACTGGG 1 cut(s) 596
BpmI CTGGAG 3 cut(s) 130, 440, 497
Bpu10I CCTNAGC 1 cut(s) 581
Bsa29I ATCGAT 2 cut(s) 25, 209
BsaBI GATNNNNATC 1 cut(s) 819
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 2 cut(s) 336, 846
BsaXI ACNNNNNCTCC 4 cut(s) 413, 443, 842, 872
Bse1I ACTGG 2 cut(s) 113, 602
Bse8I GATNNNNATC 1 cut(s) 819
BseBI CCWGG 2 cut(s) 741, 847
BseCI ATCGAT 2 cut(s) 25, 209
BseDI CCNNGG 2 cut(s) 336, 846
BseGI GGATG 6 cut(s) 150, 241, 700, 715, 753, 805
BseJI GATNNNNATC 1 cut(s) 819
BseMII CTCAG 3 cut(s) 205, 440, 572
BseNI ACTGG 2 cut(s) 113, 602
BseRI GAGGAG 3 cut(s) 30, 129, 395
BseSI GKGCMC 1 cut(s) 613
BseXI GCAGC 1 cut(s) 764
BshFI GGCC 1 cut(s) 341
BshNI GGYRCC 2 cut(s) 553, 736
BshVI ATCGAT 2 cut(s) 25, 209
BsiHKAI GWGCWC 2 cut(s) 613, 620
BsiSI CCGG 1 cut(s) 138
BsmAI GTCTC 3 cut(s) 70, 113, 264
BsnI GGCC 1 cut(s) 341
Bso31I GGTCTC 1 cut(s) 113
Bsp1286I GDGCHC 3 cut(s) 394, 613, 620
Bsp143I GATC 4 cut(s) 26, 177, 820, 906
BspACI CCGC 2 cut(s) 459, 720
BspANI GGCC 1 cut(s) 341
BspCNI CTCAG 3 cut(s) 204, 439, 573
BspDI ATCGAT 2 cut(s) 25, 209
BspLI GGNNCC 3 cut(s) 423, 555, 738
BspMAI CTGCAG 1 cut(s) 348
BspMI ACCTGC 2 cut(s) 565, 585
BspPI GGATC 2 cut(s) 828, 914
BspT107I GGYRCC 2 cut(s) 553, 736
BspTNI GGTCTC 1 cut(s) 113
BsrI ACTGG 2 cut(s) 113, 602
BssECI CCNNGG 2 cut(s) 336, 846
BssMI GATC 4 cut(s) 26, 177, 820, 906
Bst2UI CCWGG 2 cut(s) 741, 847
Bst4CI ACNGT 2 cut(s) 51, 93
Bst6I CTCTTC 1 cut(s) 931
BstC8I GCNNGC 3 cut(s) 457, 588, 651
BstDEI CTNAG 4 cut(s) 191, 426, 581, 871
BstDSI CCRYGG 1 cut(s) 336
BstF5I GGATG 6 cut(s) 150, 241, 700, 715, 753, 805
BstH2I RGCGCY 1 cut(s) 504
BstHHI GCGC 1 cut(s) 503
BstKTI GATC 4 cut(s) 29, 180, 823, 909
BstMAI GTCTC 3 cut(s) 70, 113, 264
BstMBI GATC 4 cut(s) 26, 177, 820, 906
BstMWI GCNNNNNNNGC 1 cut(s) 566
BstNI CCWGG 2 cut(s) 741, 847
BstNSI RCATGY 1 cut(s) 590
BstSCI CCNGG 2 cut(s) 739, 845
BstSFI CTRYAG 1 cut(s) 344
BstSLI GKGCMC 1 cut(s) 613
BstV1I GCAGC 1 cut(s) 764
BstXI CCANNNNNNTGG 1 cut(s) 475
Bsu15I ATCGAT 2 cut(s) 25, 209
BsuRI GGCC 1 cut(s) 341
BsuTUI ATCGAT 2 cut(s) 25, 209
BtgI CCRYGG 1 cut(s) 336
BtsCI GGATG 6 cut(s) 150, 241, 700, 715, 753, 805
BtsI GCAGTG 1 cut(s) 762
BtsIMutI CAGTG 2 cut(s) 609, 762
BveI ACCTGC 2 cut(s) 565, 585
Cac8I GCNNGC 3 cut(s) 457, 588, 651
CfoI GCGC 1 cut(s) 503
Cfr13I GGNCC 2 cut(s) 340, 713
ClaI ATCGAT 2 cut(s) 25, 209
CseI GACGC 1 cut(s) 494
Csp6I GTAC 5 cut(s) 47, 106, 188, 554, 803
CviAII CATG 5 cut(s) 127, 276, 294, 587, 812
CviQI GTAC 5 cut(s) 47, 106, 188, 554, 803
DdeI CTNAG 4 cut(s) 191, 426, 581, 871
DpnI GATC 4 cut(s) 28, 179, 822, 908
DpnII GATC 4 cut(s) 26, 177, 820, 906
Eam1104I CTCTTC 1 cut(s) 931
EarI CTCTTC 1 cut(s) 931
Ecl136II GAGCTC 1 cut(s) 618
Eco24I GRGCYC 2 cut(s) 394, 620
Eco31I GGTCTC 1 cut(s) 113
Eco47I GGWCC 1 cut(s) 713
Eco47III AGCGCT 1 cut(s) 502
Eco53kI GAGCTC 1 cut(s) 618
Eco57I CTGAAG 1 cut(s) 93
EcoICRI GAGCTC 1 cut(s) 618
EcoRII CCWGG 2 cut(s) 739, 845
EcoT38I GRGCYC 2 cut(s) 394, 620
FaeI CATG 5 cut(s) 130, 279, 297, 590, 815
FatI CATG 5 cut(s) 126, 275, 293, 586, 811
FauI CCCGC 1 cut(s) 713
Fnu4HI GCNGC 2 cut(s) 460, 753
FokI GGATG 6 cut(s) 137, 228, 707, 722, 760, 812
FriOI GRGCYC 2 cut(s) 394, 620
Fsp4HI GCNGC 2 cut(s) 460, 753
FspBI CTAG 2 cut(s) 44, 806
GlaI GCGC 1 cut(s) 502
GluI GCNGC 2 cut(s) 460, 753
GsuI CTGGAG 3 cut(s) 130, 440, 497
HaeII RGCGCY 1 cut(s) 504
HaeIII GGCC 1 cut(s) 341
HapII CCGG 1 cut(s) 138
HgaI GACGC 1 cut(s) 494
HhaI GCGC 1 cut(s) 503
Hin1II CATG 5 cut(s) 130, 279, 297, 590, 815
Hin6I GCGC 1 cut(s) 501
HinP1I GCGC 1 cut(s) 501
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HinfI GANTC 3 cut(s) 272, 515, 531
HpaI GTTAAC 1 cut(s) 331
HpaII CCGG 1 cut(s) 138
HphI GGTGA 1 cut(s) 60
Hpy166II GTNNAC 3 cut(s) 331, 544, 611
Hpy188I TCNGA 3 cut(s) 194, 514, 640
Hpy188III TCNNGA 1 cut(s) 476
Hpy8I GTNNAC 3 cut(s) 331, 544, 611
Hpy99I CGWCG 1 cut(s) 488
HpyAV CCTTC 2 cut(s) 69, 297
HpyCH4III ACNGT 2 cut(s) 51, 93
HpyCH4V TGCA 8 cut(s) 297, 346, 455, 560, 590, 611, 767, 811
HpyF10VI GCNNNNNNNGC 1 cut(s) 566
HpyF3I CTNAG 4 cut(s) 191, 426, 581, 871
Hsp92II CATG 5 cut(s) 130, 279, 297, 590, 815
HspAI GCGC 1 cut(s) 501
KpnI GGTACC 1 cut(s) 557
KspAI GTTAAC 1 cut(s) 331
Kzo9I GATC 4 cut(s) 26, 177, 820, 906
LmnI GCTCC 2 cut(s) 174, 421
Lsp1109I GCAGC 1 cut(s) 764
MaeI CTAG 2 cut(s) 44, 806
MaeIII GTNAC 3 cut(s) 34, 398, 952
MalI GATC 4 cut(s) 28, 179, 822, 908
MboI GATC 4 cut(s) 26, 177, 820, 906
MboII GAAGA 8 cut(s) 21, 213, 216, 219, 228, 282, 870, 918
MfeI CAATTG 1 cut(s) 828
MhlI GDGCHC 3 cut(s) 394, 613, 620
MluCI AATT 5 cut(s) 302, 661, 828, 890, 932
MlyI GAGTC 1 cut(s) 266
MmeI TCCRAC 1 cut(s) 543
MseI TTAA 2 cut(s) 330, 687
MspI CCGG 1 cut(s) 138
MspR9I CCNGG 2 cut(s) 741, 847
MunI CAATTG 1 cut(s) 828
MvaI CCWGG 2 cut(s) 741, 847
MwoI GCNNNNNNNGC 1 cut(s) 566
NdeII GATC 4 cut(s) 26, 177, 820, 906
NlaIII CATG 5 cut(s) 130, 279, 297, 590, 815
NlaIV GGNNCC 3 cut(s) 423, 555, 738
NspI RCATGY 1 cut(s) 590
PaeI GCATGC 1 cut(s) 590
PfeI GAWTC 2 cut(s) 515, 531
PkrI GCNGC 2 cut(s) 461, 754
PleI GAGTC 1 cut(s) 266
PpsI GAGTC 1 cut(s) 266
PsiI TTATAA 1 cut(s) 573
Psp124BI GAGCTC 1 cut(s) 620
Psp6I CCWGG 2 cut(s) 739, 845
PspGI CCWGG 2 cut(s) 739, 845
PspN4I GGNNCC 3 cut(s) 423, 555, 738
PspPI GGNCC 2 cut(s) 340, 713
PstI CTGCAG 1 cut(s) 348
RsaI GTAC 5 cut(s) 48, 107, 189, 555, 804
RsaNI GTAC 5 cut(s) 47, 106, 188, 554, 803
SacI GAGCTC 1 cut(s) 620
SaqAI TTAA 2 cut(s) 330, 687
SatI GCNGC 2 cut(s) 460, 753
Sau3AI GATC 4 cut(s) 26, 177, 820, 906
Sau96I GGNCC 2 cut(s) 340, 713
ScaI AGTACT 1 cut(s) 107
SchI GAGTC 1 cut(s) 266
ScrFI CCNGG 2 cut(s) 741, 847
SduI GDGCHC 3 cut(s) 394, 613, 620
SfcI CTRYAG 1 cut(s) 344
SinI GGWCC 1 cut(s) 713
SpeI ACTAGT 1 cut(s) 805
SphI GCATGC 1 cut(s) 590
Sse9I AATT 5 cut(s) 302, 661, 828, 890, 932
SsiI CCGC 2 cut(s) 459, 720
SspI AATATT 1 cut(s) 793
SspMI CTAG 2 cut(s) 44, 806
SstI GAGCTC 1 cut(s) 620
StyD4I CCNGG 2 cut(s) 739, 845
TaaI ACNGT 2 cut(s) 51, 93
TaqI TCGA 4 cut(s) 25, 176, 209, 823
TasI AATT 5 cut(s) 302, 661, 828, 890, 932
TatI WGTACW 3 cut(s) 105, 187, 802
TauI GCSGC 1 cut(s) 462
TfiI GAWTC 2 cut(s) 515, 531
Tru1I TTAA 2 cut(s) 330, 687
Tru9I TTAA 2 cut(s) 330, 687
TscAI CASTG 2 cut(s) 609, 769
TseI GCWGC 1 cut(s) 752
TspDTI ATGAA 2 cut(s) 143, 282
TspGWI ACGGA 2 cut(s) 316, 812
TspRI CASTG 2 cut(s) 609, 769
VneI GTGCAC 1 cut(s) 609
VpaK11BI GGWCC 1 cut(s) 713
XapI RAATTY 3 cut(s) 302, 661, 932
XceI RCATGY 1 cut(s) 590
XspI CTAG 2 cut(s) 44, 806
ZrmI AGTACT 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.