Rw7G009300

Polysaccharide biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014552
Physical Location & Seq
Forward (+)
8742234 .. 8743199
966 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G009300.1

Sequence Viewer

Length: 966 bp
ATGCCCCCAGAAGTGCCCCATTATCGATCTTCTGTAACTCCTCTAGTACGGTTCTCTCCTTCTATCTCACCTGAAGCCCAACTAAATCAACTGTACAGACACAAGTATTGGAGAGGAGAGAAAGGAGAGACAAATATGAACTTTGCCAGAAAGAAACTCATCCCTTTTCTTGTTTTAATCCTTTCAATCATTTCTGTACTCAGACTTATTAGAGTAGCAATAACTACATCTTCTTCTTCACACCCATTGCCTGCTTTACCTCCTCTTGGAGACTCATGTTCCAATCCTCCCTCATGCACTAAATTTCAATCAGAGAGAAGTGCCTCCATTAACGCCACCCGCCTGCGGAAAAAGGAATACCAACTTCTGTCAAATCTCATTTCCCAGAGAGCCCCTTGCAACCTCCTCATTTTTGGGCTGGAACCTCAGTATCTATCTCTCTCATCAATCAATGCAGGCGGCACTACCATCTTTCTGGAGGATGACGTTAACAAGATAAGCACCATGACAACAGATTCCAACACCACCAGAATCTACAGGGTGGACTACGAGGTACCTGCAAAAGAGGCCTACAACCTGCTGAAGCATGCAAGGGAAAACCCAGTCTGTGCACCTAGCTCAAAACAGCTTCAAGTATCAAAATGTCAGCTTGCGCTGAAAAATTTGCCACAAGAAGTTTATGAGCTTAAATGGGATGTGGTGGTGGTGGATGGACCAAGCGGGAATAGGCCAGAGGCACCAGGCAGGATGGCAGCTATATATACTGCAAGTATGTTAGCAAGAAATGGGAATATTACGGATGTAGTAGTGCATGATGTGGATCGAACAATTGAGAAGTGGTTTTCCTGGGAATTTCTGTGTGATGAGAACTTGGTTTCTTCAAAAGGGAAACTATGGAACTTTAGGATCATGGGTCACACAAATTCTTCAAAATTTTGCCTTCCCAAAGAAGTTACAATAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polysacc_synt_4

Protein Analysis

321

Amino Acids

36.08

Weight (kDa)

9.18

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 119 - 301 6.2e-69 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014552)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24910
fragaria_vesca FvH4_5g08380
malus_domestica MD06G1141300.v1.1 MD14G1156500.v1.1
prunus_persica Prupe.5G150600_v2.0.a1
pyrus_communis pycom06g13130 pycom14g13010
rosa_chinensis RchiOBHm_Chr7g0190071
rosa_laevigata RLG00000004567
rosa_multiflora Rmu_sc0004886.1_g000008
rosa_roxburghii Rroxscaffold_3G00264910
rosa_rugosa Rorug06G0502200
rosa_samantha Rh7AG107800 Rh7BG110100 Rh7CG112300 Rh7DG110700
rosa_wichuraiana Rw7G009300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 565, 585
Acc65I GGTACC 1 cut(s) 553
AccB1I GGYRCC 2 cut(s) 553, 736
AciI CCGC 4 cut(s) 340, 346, 459, 720
AclWI GGATC 2 cut(s) 828, 914
AcsI RAATTY 5 cut(s) 302, 661, 851, 922, 932
AcuI CTGAAG 2 cut(s) 93, 602
AfaI GTAC 4 cut(s) 48, 95, 198, 555
AfiI CCNNNNNNNGG 2 cut(s) 266, 345
AgsI TTSAA 5 cut(s) 186, 308, 632, 882, 930
AjnI CCWGG 2 cut(s) 739, 845
AluBI AGCT 5 cut(s) 618, 628, 649, 685, 755
AluI AGCT 5 cut(s) 618, 628, 649, 685, 755
Alw21I GWGCWC 1 cut(s) 613
Alw26I GTCTC 2 cut(s) 122, 264
Alw44I GTGCAC 1 cut(s) 609
AlwI GGATC 2 cut(s) 828, 914
AoxI GGCC 2 cut(s) 567, 728
ApaLI GTGCAC 1 cut(s) 609
ApeKI GCWGC 1 cut(s) 752
ApoI RAATTY 5 cut(s) 302, 661, 851, 922, 932
Asp718I GGTACC 1 cut(s) 553
AspLEI GCGC 1 cut(s) 655
AspS9I GGNCC 1 cut(s) 713
AsuHPI GGTGA 1 cut(s) 60
AvaII GGWCC 1 cut(s) 713
BaeGI GKGCMC 2 cut(s) 18, 613
BaeI ACNNNNGTAYC 2 cut(s) 413, 446
BanI GGYRCC 2 cut(s) 553, 736
BanII GRGCYC 1 cut(s) 394
Bbv12I GWGCWC 1 cut(s) 613
BbvI GCAGC 1 cut(s) 764
BccI CCATC 3 cut(s) 476, 704, 742
BcgI CGANNNNNNTGC 2 cut(s) 539, 573
BciT130I CCWGG 2 cut(s) 741, 847
BcoDI GTCTC 2 cut(s) 122, 264
BfaI CTAG 2 cut(s) 44, 615
BfmI CTRYAG 1 cut(s) 535
BfuAI ACCTGC 2 cut(s) 565, 585
BisI GCNGC 2 cut(s) 460, 753
BlsI GCNGC 2 cut(s) 461, 754
Bme1390I CCNGG 2 cut(s) 741, 847
Bme18I GGWCC 1 cut(s) 713
BmgT120I GGNCC 1 cut(s) 713
BmiI GGNNCC 3 cut(s) 423, 555, 738
BmrFI CCNGG 2 cut(s) 741, 847
BmrI ACTGGG 1 cut(s) 596
BmuI ACTGGG 1 cut(s) 596
BpmI CTGGAG 1 cut(s) 497
Bsa29I ATCGAT 1 cut(s) 25
BsaBI GATNNNNATC 1 cut(s) 819
BsaJI CCNNGG 1 cut(s) 846
Bsc4I CCNNNNNNNGG 2 cut(s) 266, 345
Bse1I ACTGG 1 cut(s) 602
Bse3DI GCAATG 1 cut(s) 245
Bse8I GATNNNNATC 1 cut(s) 819
BseBI CCWGG 2 cut(s) 741, 847
BseCI ATCGAT 1 cut(s) 25
BseDI CCNNGG 1 cut(s) 846
BseGI GGATG 6 cut(s) 159, 487, 700, 715, 753, 805
BseJI GATNNNNATC 1 cut(s) 819
BseLI CCNNNNNNNGG 2 cut(s) 266, 345
BseMI GCAATG 1 cut(s) 245
BseMII CTCAG 2 cut(s) 214, 440
BseNI ACTGG 1 cut(s) 602
BseRI GAGGAG 4 cut(s) 30, 129, 252, 395
BseSI GKGCMC 2 cut(s) 18, 613
BseXI GCAGC 1 cut(s) 764
BshFI GGCC 2 cut(s) 569, 730
BshNI GGYRCC 2 cut(s) 553, 736
BshVI ATCGAT 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 613
BslI CCNNNNNNNGG 2 cut(s) 266, 345
BsmAI GTCTC 2 cut(s) 122, 264
BsnI GGCC 2 cut(s) 569, 730
Bsp1286I GDGCHC 3 cut(s) 18, 394, 613
Bsp1407I TGTACA 1 cut(s) 93
Bsp143I GATC 3 cut(s) 26, 820, 906
BspACI CCGC 4 cut(s) 340, 346, 459, 720
BspANI GGCC 2 cut(s) 569, 730
BspCNI CTCAG 2 cut(s) 213, 439
BspDI ATCGAT 1 cut(s) 25
BspLI GGNNCC 3 cut(s) 423, 555, 738
BspMI ACCTGC 2 cut(s) 565, 585
BspPI GGATC 2 cut(s) 828, 914
BspT107I GGYRCC 2 cut(s) 553, 736
BsrDI GCAATG 1 cut(s) 245
BsrGI TGTACA 1 cut(s) 93
BsrI ACTGG 1 cut(s) 602
BssECI CCNNGG 1 cut(s) 846
BssMI GATC 3 cut(s) 26, 820, 906
Bst2UI CCWGG 2 cut(s) 741, 847
Bst4CI ACNGT 2 cut(s) 51, 93
BstAUI TGTACA 1 cut(s) 93
BstC8I GCNNGC 5 cut(s) 252, 344, 457, 588, 651
BstDEI CTNAG 2 cut(s) 200, 426
BstF5I GGATG 6 cut(s) 159, 487, 700, 715, 753, 805
BstHHI GCGC 1 cut(s) 655
BstKTI GATC 3 cut(s) 29, 823, 909
BstMAI GTCTC 2 cut(s) 122, 264
BstMBI GATC 3 cut(s) 26, 820, 906
BstMWI GCNNNNNNNGC 1 cut(s) 566
BstNI CCWGG 2 cut(s) 741, 847
BstNSI RCATGY 1 cut(s) 590
BstSCI CCNGG 2 cut(s) 739, 845
BstSFI CTRYAG 1 cut(s) 535
BstSLI GKGCMC 2 cut(s) 18, 613
BstV1I GCAGC 1 cut(s) 764
BstXI CCANNNNNNTGG 1 cut(s) 475
Bsu15I ATCGAT 1 cut(s) 25
BsuRI GGCC 2 cut(s) 569, 730
BsuTUI ATCGAT 1 cut(s) 25
BtsCI GGATG 6 cut(s) 159, 487, 700, 715, 753, 805
BveI ACCTGC 2 cut(s) 565, 585
Cac8I GCNNGC 5 cut(s) 252, 344, 457, 588, 651
CfoI GCGC 1 cut(s) 655
Cfr13I GGNCC 1 cut(s) 713
ClaI ATCGAT 1 cut(s) 25
Csp6I GTAC 4 cut(s) 47, 94, 197, 554
CviAII CATG 6 cut(s) 276, 294, 505, 587, 812, 910
CviQI GTAC 4 cut(s) 47, 94, 197, 554
DdeI CTNAG 2 cut(s) 200, 426
DpnI GATC 3 cut(s) 28, 822, 908
DpnII GATC 3 cut(s) 26, 820, 906
Eco147I AGGCCT 1 cut(s) 569
Eco24I GRGCYC 1 cut(s) 394
Eco47I GGWCC 1 cut(s) 713
Eco57I CTGAAG 2 cut(s) 93, 602
EcoRII CCWGG 2 cut(s) 739, 845
EcoT38I GRGCYC 1 cut(s) 394
FaeI CATG 6 cut(s) 279, 297, 508, 590, 815, 913
FatI CATG 6 cut(s) 275, 293, 504, 586, 811, 909
FauI CCCGC 2 cut(s) 347, 713
Fnu4HI GCNGC 2 cut(s) 460, 753
FokI GGATG 6 cut(s) 146, 494, 707, 722, 760, 812
FriOI GRGCYC 1 cut(s) 394
Fsp4HI GCNGC 2 cut(s) 460, 753
FspBI CTAG 2 cut(s) 44, 615
GlaI GCGC 1 cut(s) 654
GluI GCNGC 2 cut(s) 460, 753
GsuI CTGGAG 1 cut(s) 497
HaeIII GGCC 2 cut(s) 569, 730
HhaI GCGC 1 cut(s) 655
Hin1II CATG 6 cut(s) 279, 297, 508, 590, 815, 913
Hin6I GCGC 1 cut(s) 653
HinP1I GCGC 1 cut(s) 653
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HinfI GANTC 3 cut(s) 272, 515, 531
HpaI GTTAAC 1 cut(s) 490
HphI GGTGA 1 cut(s) 60
Hpy166II GTNNAC 3 cut(s) 490, 544, 611
Hpy188I TCNGA 2 cut(s) 203, 313
Hpy188III TCNNGA 1 cut(s) 476
Hpy8I GTNNAC 3 cut(s) 490, 544, 611
HpyAV CCTTC 2 cut(s) 69, 950
HpyCH4III ACNGT 2 cut(s) 51, 93
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 8 cut(s) 297, 399, 455, 560, 590, 611, 767, 811
HpyF10VI GCNNNNNNNGC 1 cut(s) 566
HpyF3I CTNAG 2 cut(s) 200, 426
HpySE526I ACGT 1 cut(s) 486
Hsp92II CATG 6 cut(s) 279, 297, 508, 590, 815, 913
HspAI GCGC 1 cut(s) 653
KpnI GGTACC 1 cut(s) 557
KspAI GTTAAC 1 cut(s) 490
Kzo9I GATC 3 cut(s) 26, 820, 906
Lsp1109I GCAGC 1 cut(s) 764
MaeI CTAG 2 cut(s) 44, 615
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 3 cut(s) 34, 914, 952
MalI GATC 3 cut(s) 28, 822, 908
MboI GATC 3 cut(s) 26, 820, 906
MboII GAAGA 6 cut(s) 21, 222, 225, 228, 870, 918
MfeI CAATTG 1 cut(s) 828
MhlI GDGCHC 3 cut(s) 18, 394, 613
MluCI AATT 6 cut(s) 302, 661, 828, 851, 922, 932
MlyI GAGTC 1 cut(s) 266
MmeI TCCRAC 1 cut(s) 543
MseI TTAA 4 cut(s) 176, 330, 489, 687
MspR9I CCNGG 2 cut(s) 741, 847
MunI CAATTG 1 cut(s) 828
MvaI CCWGG 2 cut(s) 741, 847
MwoI GCNNNNNNNGC 1 cut(s) 566
NdeII GATC 3 cut(s) 26, 820, 906
NlaIII CATG 6 cut(s) 279, 297, 508, 590, 815, 913
NlaIV GGNNCC 3 cut(s) 423, 555, 738
NmuCI GTSAC 1 cut(s) 914
NspI RCATGY 1 cut(s) 590
PaeI GCATGC 1 cut(s) 590
PceI AGGCCT 1 cut(s) 569
PfeI GAWTC 2 cut(s) 515, 531
PkrI GCNGC 2 cut(s) 461, 754
PleI GAGTC 1 cut(s) 266
PpsI GAGTC 1 cut(s) 266
Psp6I CCWGG 2 cut(s) 739, 845
PspGI CCWGG 2 cut(s) 739, 845
PspN4I GGNNCC 3 cut(s) 423, 555, 738
PspPI GGNCC 1 cut(s) 713
RsaI GTAC 4 cut(s) 48, 95, 198, 555
RsaNI GTAC 4 cut(s) 47, 94, 197, 554
SaqAI TTAA 4 cut(s) 176, 330, 489, 687
SatI GCNGC 2 cut(s) 460, 753
Sau3AI GATC 3 cut(s) 26, 820, 906
Sau96I GGNCC 1 cut(s) 713
SchI GAGTC 1 cut(s) 266
ScrFI CCNGG 2 cut(s) 741, 847
SduI GDGCHC 3 cut(s) 18, 394, 613
SfcI CTRYAG 1 cut(s) 535
SinI GGWCC 1 cut(s) 713
SphI GCATGC 1 cut(s) 590
Sse9I AATT 6 cut(s) 302, 661, 828, 851, 922, 932
SseBI AGGCCT 1 cut(s) 569
SsiI CCGC 4 cut(s) 340, 346, 459, 720
SspI AATATT 1 cut(s) 793
SspMI CTAG 2 cut(s) 44, 615
StuI AGGCCT 1 cut(s) 569
StyD4I CCNGG 2 cut(s) 739, 845
TaaI ACNGT 2 cut(s) 51, 93
TaiI ACGT 1 cut(s) 489
TaqI TCGA 2 cut(s) 25, 823
TasI AATT 6 cut(s) 302, 661, 828, 851, 922, 932
TatI WGTACW 2 cut(s) 93, 196
TauI GCSGC 1 cut(s) 462
TfiI GAWTC 2 cut(s) 515, 531
Tru1I TTAA 4 cut(s) 176, 330, 489, 687
Tru9I TTAA 4 cut(s) 176, 330, 489, 687
TseFI GTSAC 1 cut(s) 914
TseI GCWGC 1 cut(s) 752
Tsp45I GTSAC 1 cut(s) 914
TspDTI ATGAA 1 cut(s) 152
TspGWI ACGGA 1 cut(s) 812
VneI GTGCAC 1 cut(s) 609
VpaK11BI GGWCC 1 cut(s) 713
XapI RAATTY 5 cut(s) 302, 661, 851, 922, 932
XceI RCATGY 1 cut(s) 590
XspI CTAG 2 cut(s) 44, 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.