MD14G1156500.v1.1

Polysaccharide biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014552
Physical Location & Seq
Forward (+)
25093974 .. 25094909
936 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1156500.v1.1.491

Sequence Viewer

Length: 858 bp
ATGCCCCCCGAAGTGCCTCATTTTTGCTCTTTCGTGGCTCCTCTCGTTCGGTTCACTTCCGCAGTCTCACCGCAAGAACAAGAGAACAAGGCTCATAGACACAACCAATCAAGAGGATCTAAAGCCATGAACTTTTCCAGAACAAAACTCATCCCTTTCCTTGTTTTGATCCTTTCGATTGTTTCGGTGCTCAGACTTGTTAGAATTGCAGTAACTACTTCTCCTCCTCCTCCTCCTACTCTTGGAAAAACATGTTCTTTCCCTTTCATATGCACCAAGTTTCCGGCAGAAACAAACGCATCTGCCAACACCGCCGCCCTCAGGAAAAAGGAATACCAACTGCTGTCGAATCTCATTTCGCAGAAAGCTCCCTGCAACCTCCTCATATTCGGGCTTGAACCTCAGTACGTATCTCTCTCATCAATCAATACAGGCGGCACCACCGTCTTTATACCTGCAAAAGAGGCTTACAAGCTGCTCCAGCATGCAAGGGGAAACCCAGATTGCAAACCGAAACTGCTTCAACAATCAAAATGTCAACTTGCATTGAAAAACCTACCGCAAGAAGTCTACGAGCTTAAATGGGATGTGGTGGTGGTGGATGGACCGAGCGGGAACACACCAGAGGAACCGGGTAGGATGTCATCAATCTATTCTGCTAGTATGATAGCAAGAAATGCAAACATTACGCATGTCGTTGTGCATGACGTAGATCGAATGATCGAGAAGTGGTTTTCCTGGGAGTTTCTGTGTGATGAGAACTTAGTTTCTTCAAAAAGGAGATTATGGAACTTTAGGATCACAGGCCAATTAAATTCTACAATTTTTTGCCCTGCCAAAGAGATTACGATAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polysacc_synt_4

Protein Analysis

286

Amino Acids

31.87

Weight (kDa)

9.28

Isoelectric Point (pI)

52.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 111 - 155 7.9e-09 IRX15/IRX15L/GXM
IRX15_IRX15L_GXM PF21729 153 - 265 2.2e-41 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014552)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24910
fragaria_vesca FvH4_5g08380
malus_domestica MD06G1141300.v1.1 MD14G1156500.v1.1
prunus_persica Prupe.5G150600_v2.0.a1
pyrus_communis pycom06g13130 pycom14g13010
rosa_chinensis RchiOBHm_Chr7g0190071
rosa_laevigata RLG00000004567
rosa_multiflora Rmu_sc0004886.1_g000008
rosa_roxburghii Rroxscaffold_3G00264910
rosa_rugosa Rorug06G0502200
rosa_samantha Rh7AG107800 Rh7BG110100 Rh7CG112300 Rh7DG110700
rosa_wichuraiana Rw7G009300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 463
AccB1I GGYRCC 1 cut(s) 437
AccBSI CCGCTC 1 cut(s) 612
AccI GTMKAC 1 cut(s) 570
AciI CCGC 7 cut(s) 60, 71, 312, 315, 435, 560, 612
AclWI GGATC 3 cut(s) 124, 163, 806
AcsI RAATTY 1 cut(s) 814
AfaI GTAC 1 cut(s) 407
AfiI CCNNNNNNNGG 2 cut(s) 242, 321
AflIII ACRYGT 1 cut(s) 251
AgsI TTSAA 4 cut(s) 398, 524, 550, 774
AjnI CCWGG 1 cut(s) 737
AluBI AGCT 3 cut(s) 368, 475, 577
AluI AGCT 3 cut(s) 368, 475, 577
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 1 cut(s) 70
AlwI GGATC 3 cut(s) 124, 163, 806
AoxI GGCC 1 cut(s) 805
ApeKI GCWGC 1 cut(s) 475
ApoI RAATTY 1 cut(s) 814
AspS9I GGNCC 1 cut(s) 605
AsuC2I CCSGG 1 cut(s) 633
AsuHPI GGTGA 1 cut(s) 60
AvaII GGWCC 1 cut(s) 605
AxyI CCTNAGG 1 cut(s) 320
BanI GGYRCC 1 cut(s) 437
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 1 cut(s) 462
BccI CCATC 1 cut(s) 596
BciT130I CCWGG 1 cut(s) 739
BcnI CCSGG 1 cut(s) 633
BcoDI GTCTC 1 cut(s) 70
BfaI CTAG 1 cut(s) 660
BfuAI ACCTGC 1 cut(s) 463
BisI GCNGC 3 cut(s) 315, 436, 476
BlsI GCNGC 3 cut(s) 316, 437, 477
Bme1390I CCNGG 2 cut(s) 633, 739
Bme18I GGWCC 1 cut(s) 605
BmgT120I GGNCC 1 cut(s) 605
BmiI GGNNCC 3 cut(s) 39, 439, 630
BmrFI CCNGG 2 cut(s) 633, 739
BmsI GCATC 1 cut(s) 308
BpmI CTGGAG 1 cut(s) 464
BpuMI CCSGG 1 cut(s) 633
BsaAI YACGTR 1 cut(s) 409
BsaJI CCNNGG 1 cut(s) 738
BsaXI ACNNNNNCTCC 6 cut(s) 205, 208, 235, 238, 734, 764
Bsc4I CCNNNNNNNGG 2 cut(s) 242, 321
Bse21I CCTNAGG 1 cut(s) 320
BseBI CCWGG 1 cut(s) 739
BseDI CCNNGG 1 cut(s) 738
BseGI GGATG 4 cut(s) 150, 592, 607, 645
BseLI CCNNNNNNNGG 2 cut(s) 242, 321
BseMII CTCAG 3 cut(s) 205, 334, 416
BseRI GAGGAG 6 cut(s) 30, 213, 216, 219, 222, 371
BseXI GCAGC 1 cut(s) 462
BshFI GGCC 1 cut(s) 807
BshNI GGYRCC 1 cut(s) 437
BsiHKAI GWGCWC 1 cut(s) 192
BsiSI CCGG 2 cut(s) 284, 632
BslI CCNNNNNNNGG 2 cut(s) 242, 321
BsmAI GTCTC 1 cut(s) 70
BsnI GGCC 1 cut(s) 807
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 5 cut(s) 116, 168, 712, 720, 798
BspACI CCGC 7 cut(s) 60, 71, 312, 315, 435, 560, 612
BspANI GGCC 1 cut(s) 807
BspCNI CTCAG 3 cut(s) 204, 333, 415
BspLI GGNNCC 3 cut(s) 39, 439, 630
BspMI ACCTGC 1 cut(s) 463
BspPI GGATC 3 cut(s) 124, 163, 806
BspT107I GGYRCC 1 cut(s) 437
BsrBI CCGCTC 1 cut(s) 612
BssECI CCNNGG 1 cut(s) 738
BssMI GATC 5 cut(s) 116, 168, 712, 720, 798
Bst2UI CCWGG 1 cut(s) 739
Bst4CI ACNGT 1 cut(s) 445
BstAPI GCANNNNNTGC 1 cut(s) 677
BstBAI YACGTR 1 cut(s) 409
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 4 cut(s) 191, 320, 402, 763
BstF5I GGATG 4 cut(s) 150, 592, 607, 645
BstKTI GATC 5 cut(s) 119, 171, 715, 723, 801
BstMAI GTCTC 1 cut(s) 70
BstMBI GATC 5 cut(s) 116, 168, 712, 720, 798
BstMWI GCNNNNNNNGC 4 cut(s) 311, 464, 481, 677
BstNI CCWGG 1 cut(s) 739
BstNSI RCATGY 3 cut(s) 255, 488, 695
BstSCI CCNGG 2 cut(s) 631, 737
BstSNI TACGTA 1 cut(s) 409
BstV1I GCAGC 1 cut(s) 462
BstX2I RGATCY 1 cut(s) 116
BstYI RGATCY 1 cut(s) 116
Bsu36I CCTNAGG 1 cut(s) 320
BsuRI GGCC 1 cut(s) 807
BtsCI GGATG 4 cut(s) 150, 592, 607, 645
BveI ACCTGC 1 cut(s) 463
Cac8I GCNNGC 1 cut(s) 486
Cfr13I GGNCC 1 cut(s) 605
Csp6I GTAC 1 cut(s) 406
CviAII CATG 5 cut(s) 127, 252, 485, 692, 704
CviJI RGCY 9 cut(s) 38, 92, 125, 368, 394, 467, 475, 577, 807
CviKI_1 RGCY 9 cut(s) 38, 92, 125, 368, 394, 467, 475, 577, 807
CviQI GTAC 1 cut(s) 406
DdeI CTNAG 4 cut(s) 191, 320, 402, 763
DpnI GATC 5 cut(s) 118, 170, 714, 722, 800
DpnII GATC 5 cut(s) 116, 168, 712, 720, 798
Eco105I TACGTA 1 cut(s) 409
Eco47I GGWCC 1 cut(s) 605
Eco81I CCTNAGG 1 cut(s) 320
EcoRII CCWGG 1 cut(s) 737
FaeI CATG 5 cut(s) 130, 255, 488, 695, 707
FatI CATG 5 cut(s) 126, 251, 484, 691, 703
FauI CCCGC 1 cut(s) 605
FauNDI CATATG 1 cut(s) 269
FblI GTMKAC 1 cut(s) 570
Fnu4HI GCNGC 3 cut(s) 315, 436, 476
FokI GGATG 4 cut(s) 137, 599, 614, 652
Fsp4HI GCNGC 3 cut(s) 315, 436, 476
FspBI CTAG 1 cut(s) 660
GluI GCNGC 3 cut(s) 315, 436, 476
GsuI CTGGAG 1 cut(s) 464
HaeIII GGCC 1 cut(s) 807
HapII CCGG 2 cut(s) 284, 632
Hin1II CATG 5 cut(s) 130, 255, 488, 695, 707
HincII GTYRAC 1 cut(s) 539
HindII GTYRAC 1 cut(s) 539
HinfI GANTC 1 cut(s) 349
HpaII CCGG 2 cut(s) 284, 632
HphI GGTGA 1 cut(s) 60
Hpy166II GTNNAC 3 cut(s) 54, 539, 571
Hpy188I TCNGA 1 cut(s) 194
Hpy188III TCNNGA 4 cut(s) 111, 138, 322, 724
Hpy8I GTNNAC 3 cut(s) 54, 539, 571
HpyCH4III ACNGT 1 cut(s) 445
HpyCH4IV ACGT 2 cut(s) 408, 708
HpyCH4V TGCA 9 cut(s) 209, 273, 375, 458, 488, 507, 545, 680, 703
HpyF10VI GCNNNNNNNGC 4 cut(s) 311, 464, 481, 677
HpyF3I CTNAG 4 cut(s) 191, 320, 402, 763
HpySE526I ACGT 2 cut(s) 408, 708
Hsp92II CATG 5 cut(s) 130, 255, 488, 695, 707
Kzo9I GATC 5 cut(s) 116, 168, 712, 720, 798
LmnI GCTCC 3 cut(s) 43, 373, 483
Lsp1109I GCAGC 1 cut(s) 462
LweI GCATC 1 cut(s) 308
MaeI CTAG 1 cut(s) 660
MaeII ACGT 2 cut(s) 408, 708
MaeIII GTNAC 1 cut(s) 211
MalI GATC 5 cut(s) 118, 170, 714, 722, 800
MbiI CCGCTC 1 cut(s) 612
MboI GATC 5 cut(s) 116, 168, 712, 720, 798
MboII GAAGA 1 cut(s) 762
MflI RGATCY 1 cut(s) 116
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 4 cut(s) 204, 809, 814, 822
MseI TTAA 2 cut(s) 579, 812
MspI CCGG 2 cut(s) 284, 632
MspR9I CCNGG 2 cut(s) 633, 739
MvaI CCWGG 1 cut(s) 739
MwoI GCNNNNNNNGC 4 cut(s) 311, 464, 481, 677
NciI CCSGG 1 cut(s) 633
NdeI CATATG 1 cut(s) 269
NdeII GATC 5 cut(s) 116, 168, 712, 720, 798
NlaIII CATG 5 cut(s) 130, 255, 488, 695, 707
NlaIV GGNNCC 3 cut(s) 39, 439, 630
NspI RCATGY 3 cut(s) 255, 488, 695
PaeI GCATGC 1 cut(s) 488
PciI ACATGT 1 cut(s) 251
PfeI GAWTC 1 cut(s) 349
PkrI GCNGC 3 cut(s) 316, 437, 477
Ppu21I YACGTR 1 cut(s) 409
PscI ACATGT 1 cut(s) 251
Psp6I CCWGG 1 cut(s) 737
PspGI CCWGG 1 cut(s) 737
PspN4I GGNNCC 3 cut(s) 39, 439, 630
PspPI GGNCC 1 cut(s) 605
PsuI RGATCY 1 cut(s) 116
RsaI GTAC 1 cut(s) 407
RsaNI GTAC 1 cut(s) 406
SaqAI TTAA 2 cut(s) 579, 812
SatI GCNGC 3 cut(s) 315, 436, 476
Sau3AI GATC 5 cut(s) 116, 168, 712, 720, 798
Sau96I GGNCC 1 cut(s) 605
ScrFI CCNGG 2 cut(s) 633, 739
SduI GDGCHC 1 cut(s) 192
SetI ASST 9 cut(s) 370, 381, 403, 411, 457, 477, 558, 579, 711
SfaNI GCATC 1 cut(s) 308
SinI GGWCC 1 cut(s) 605
SnaBI TACGTA 1 cut(s) 409
SphI GCATGC 1 cut(s) 488
Sse9I AATT 4 cut(s) 204, 809, 814, 822
SsiI CCGC 7 cut(s) 60, 71, 312, 315, 435, 560, 612
SspMI CTAG 1 cut(s) 660
StyD4I CCNGG 2 cut(s) 631, 737
TaaI ACNGT 1 cut(s) 445
TaiI ACGT 2 cut(s) 411, 711
TaqI TCGA 4 cut(s) 176, 347, 715, 723
TaqII GACCGA 1 cut(s) 622
TasI AATT 4 cut(s) 204, 809, 814, 822
TauI GCSGC 2 cut(s) 317, 438
TfiI GAWTC 1 cut(s) 349
Tru1I TTAA 2 cut(s) 579, 812
Tru9I TTAA 2 cut(s) 579, 812
TseI GCWGC 1 cut(s) 475
TspDTI ATGAA 2 cut(s) 143, 256
VpaK11BI GGWCC 1 cut(s) 605
XapI RAATTY 1 cut(s) 814
XceI RCATGY 3 cut(s) 255, 488, 695
XmiI GTMKAC 1 cut(s) 570
XspI CTAG 1 cut(s) 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.