MD06G1141300.v1.1

Polysaccharide biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0014552
Physical Location & Seq
Forward (+)
28499862 .. 28500817
956 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1141300.v1.1.491

Sequence Viewer

Length: 933 bp
ATGCCACCAGAAGTGCCTCATTTTCGCTCTATCGTAACTCCTCTGGTTCGGTTCTCTTCTCCCGTCTCACCAGAAGCACAAGAGTGCCAGCCTCATAGACACAAGCAATCGAGAGGATCTAAAGCCATGAACTTTTCCAGAACCAAACTCATCCCTTTCCTTGTTTTGATCCTTTCGATTGTTTCGGTGCTCAGACTTGTTAGAATTGCTGTAACTACACCTCCTCCTACTCTTGGAAAGACATGTTCTTTCTCTTCCACGTGCACCAAGTTTCGTGCAGAAACAAACGCCTCTGCCAACACCTCCGGCCTCAGGAAAAAGGAATACCAACTGCTGTTGAATCTCATTTCGCAGAAAGCTCCCTGCAACCTCCTCATATTCGGGCTTGAACCTCAGTATATATCTCTGTCATTAATCAATGCAGGCGGCATCACCGTCTTTCTAGAGGATGATCAAAAGAAGATAAGCACACTGAAACCAAATTCCAACACCACCAGAGTCTACAAGGTGGACTACCAGATACCTGCAAAAGAGGCGTACAGGCTGCTCAAGCACGCAAGGGAACACCCAGACTGCGTACCCAGCTCAAAACTGCTTCAACGATCAAAATGTCAACTTGCATTGAAAAATTTACCACAAGAAGTTTACCAGCTTAAATGGGATGTGGTGGTGGTGGATGGACCGAGCGGGAATGCACCAGAGGAACCGGGTAGGATGTCAGCAATCTATTCTGCTAGTATGATAGCAAGAAATGCAAACATTACGCATGTGGTAGTGCATGACGTAGACCGAATGATCGAGAAGTGGTTTTCCTGGGAGTTTCTGTGTGATGAGAACTTAGTTTCTTCCAAAGGGAGATTATGGAACTTTAGGATCACAGGCCAATCAAATTCTACAAATTTTTGCCCTGCCAAAGCAATTAGGATAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polysacc_synt_4

Protein Analysis

311

Amino Acids

34.83

Weight (kDa)

9.57

Isoelectric Point (pI)

43.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 108 - 290 5.2e-66 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014552)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24910
fragaria_vesca FvH4_5g08380
malus_domestica MD06G1141300.v1.1 MD14G1156500.v1.1
prunus_persica Prupe.5G150600_v2.0.a1
pyrus_communis pycom06g13130 pycom14g13010
rosa_chinensis RchiOBHm_Chr7g0190071
rosa_laevigata RLG00000004567
rosa_multiflora Rmu_sc0004886.1_g000008
rosa_roxburghii Rroxscaffold_3G00264910
rosa_rugosa Rorug06G0502200
rosa_samantha Rh7AG107800 Rh7BG110100 Rh7CG112300 Rh7DG110700
rosa_wichuraiana Rw7G009300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 532
AccBSI CCGCTC 1 cut(s) 687
AccI GTMKAC 2 cut(s) 501, 786
AciI CCGC 2 cut(s) 426, 687
AclWI GGATC 3 cut(s) 124, 163, 881
AcsI RAATTY 4 cut(s) 481, 628, 889, 898
AcvI CACGTG 1 cut(s) 261
AfaI GTAC 2 cut(s) 539, 579
AfiI CCNNNNNNNGG 2 cut(s) 233, 312
AflIII ACRYGT 1 cut(s) 242
AgsI TTSAA 4 cut(s) 340, 389, 599, 625
AjnI CCWGG 1 cut(s) 812
AleI CACNNNNGTG 1 cut(s) 82
AluBI AGCT 3 cut(s) 359, 585, 652
AluI AGCT 3 cut(s) 359, 585, 652
Alw21I GWGCWC 2 cut(s) 192, 266
Alw26I GTCTC 1 cut(s) 70
Alw44I GTGCAC 1 cut(s) 262
AlwI GGATC 3 cut(s) 124, 163, 881
AoxI GGCC 2 cut(s) 307, 880
ApaLI GTGCAC 1 cut(s) 262
ApeKI GCWGC 1 cut(s) 544
ApoI RAATTY 4 cut(s) 481, 628, 889, 898
AseI ATTAAT 1 cut(s) 413
AspS9I GGNCC 1 cut(s) 680
AsuC2I CCSGG 1 cut(s) 708
AsuHPI GGTGA 2 cut(s) 60, 424
AvaII GGWCC 1 cut(s) 680
AxyI CCTNAGG 1 cut(s) 311
BaeGI GKGCMC 1 cut(s) 266
BbrPI CACGTG 1 cut(s) 261
Bbv12I GWGCWC 2 cut(s) 192, 266
BbvI GCAGC 1 cut(s) 531
BccI CCATC 1 cut(s) 671
BciT130I CCWGG 1 cut(s) 814
BclI TGATCA 1 cut(s) 451
BcnI CCSGG 1 cut(s) 708
BcoDI GTCTC 1 cut(s) 70
BfaI CTAG 2 cut(s) 443, 735
BfuAI ACCTGC 1 cut(s) 532
BisI GCNGC 2 cut(s) 427, 545
BlsI GCNGC 2 cut(s) 428, 546
Bme1390I CCNGG 2 cut(s) 708, 814
Bme18I GGWCC 1 cut(s) 680
BmgT120I GGNCC 1 cut(s) 680
BmiI GGNNCC 1 cut(s) 705
BmrFI CCNGG 2 cut(s) 708, 814
BmsI GCATC 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 533
BpuMI CCSGG 1 cut(s) 708
BsaAI YACGTR 1 cut(s) 261
BsaJI CCNNGG 1 cut(s) 813
BsaXI ACNNNNNCTCC 6 cut(s) 205, 208, 235, 238, 809, 839
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 312
Bse21I CCTNAGG 1 cut(s) 311
BseBI CCWGG 1 cut(s) 814
BseDI CCNNGG 1 cut(s) 813
BseGI GGATG 5 cut(s) 150, 454, 667, 682, 720
BseLI CCNNNNNNNGG 2 cut(s) 233, 312
BseMII CTCAG 3 cut(s) 205, 325, 407
BseRI GAGGAG 3 cut(s) 30, 213, 362
BseSI GKGCMC 1 cut(s) 266
BseXI GCAGC 1 cut(s) 531
BseYI CCCAGC 1 cut(s) 581
BsgI GTGCAG 1 cut(s) 297
BshFI GGCC 2 cut(s) 309, 882
BsiHKAI GWGCWC 2 cut(s) 192, 266
BsiSI CCGG 2 cut(s) 306, 707
BslI CCNNNNNNNGG 2 cut(s) 233, 312
BsmAI GTCTC 1 cut(s) 70
BsmBI CGTCTC 1 cut(s) 70
BsmI GAATGC 1 cut(s) 697
BsnI GGCC 2 cut(s) 309, 882
Bsp1286I GDGCHC 2 cut(s) 192, 266
Bsp143I GATC 6 cut(s) 116, 168, 451, 602, 795, 873
BspACI CCGC 2 cut(s) 426, 687
BspANI GGCC 2 cut(s) 309, 882
BspCNI CTCAG 3 cut(s) 204, 324, 406
BspLI GGNNCC 1 cut(s) 705
BspMI ACCTGC 1 cut(s) 532
BspPI GGATC 3 cut(s) 124, 163, 881
BsrBI CCGCTC 1 cut(s) 687
BssECI CCNNGG 1 cut(s) 813
BssMI GATC 6 cut(s) 116, 168, 451, 602, 795, 873
Bst2UI CCWGG 1 cut(s) 814
Bst4CI ACNGT 1 cut(s) 436
Bst6I CTCTTC 2 cut(s) 61, 259
BstAPI GCANNNNNTGC 1 cut(s) 752
BstBAI YACGTR 1 cut(s) 261
BstC8I GCNNGC 3 cut(s) 89, 424, 555
BstDEI CTNAG 4 cut(s) 191, 311, 393, 838
BstF5I GGATG 5 cut(s) 150, 454, 667, 682, 720
BstKTI GATC 6 cut(s) 119, 171, 454, 605, 798, 876
BstMAI GTCTC 1 cut(s) 70
BstMBI GATC 6 cut(s) 116, 168, 451, 602, 795, 873
BstMWI GCNNNNNNNGC 4 cut(s) 533, 550, 582, 752
BstNI CCWGG 1 cut(s) 814
BstNSI RCATGY 2 cut(s) 246, 770
BstSCI CCNGG 2 cut(s) 706, 812
BstSLI GKGCMC 1 cut(s) 266
BstV1I GCAGC 1 cut(s) 531
BstX2I RGATCY 1 cut(s) 116
BstYI RGATCY 1 cut(s) 116
Bsu36I CCTNAGG 1 cut(s) 311
BsuRI GGCC 2 cut(s) 309, 882
BtsCI GGATG 5 cut(s) 150, 454, 667, 682, 720
BtsIMutI CAGTG 1 cut(s) 470
BveI ACCTGC 1 cut(s) 532
Cac8I GCNNGC 3 cut(s) 89, 424, 555
Cfr13I GGNCC 1 cut(s) 680
Csp6I GTAC 2 cut(s) 538, 578
CviAII CATG 4 cut(s) 127, 243, 767, 779
CviJI RGCY 9 cut(s) 91, 125, 309, 359, 385, 544, 585, 652, 882
CviKI_1 RGCY 9 cut(s) 91, 125, 309, 359, 385, 544, 585, 652, 882
CviQI GTAC 2 cut(s) 538, 578
DdeI CTNAG 4 cut(s) 191, 311, 393, 838
DpnI GATC 6 cut(s) 118, 170, 453, 604, 797, 875
DpnII GATC 6 cut(s) 116, 168, 451, 602, 795, 873
Eam1104I CTCTTC 2 cut(s) 61, 259
EarI CTCTTC 2 cut(s) 61, 259
Eco47I GGWCC 1 cut(s) 680
Eco72I CACGTG 1 cut(s) 261
Eco81I CCTNAGG 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 812
Esp3I CGTCTC 1 cut(s) 70
FaeI CATG 4 cut(s) 130, 246, 770, 782
FatI CATG 4 cut(s) 126, 242, 766, 778
FauI CCCGC 1 cut(s) 680
FbaI TGATCA 1 cut(s) 451
FblI GTMKAC 2 cut(s) 501, 786
Fnu4HI GCNGC 2 cut(s) 427, 545
FokI GGATG 5 cut(s) 137, 461, 674, 689, 727
Fsp4HI GCNGC 2 cut(s) 427, 545
FspBI CTAG 2 cut(s) 443, 735
GluI GCNGC 2 cut(s) 427, 545
GsaI CCCAGC 1 cut(s) 585
HaeIII GGCC 2 cut(s) 309, 882
HapII CCGG 2 cut(s) 306, 707
Hin1II CATG 4 cut(s) 130, 246, 770, 782
HincII GTYRAC 1 cut(s) 614
HindII GTYRAC 1 cut(s) 614
HinfI GANTC 2 cut(s) 340, 498
HpaII CCGG 2 cut(s) 306, 707
HphI GGTGA 2 cut(s) 60, 424
Hpy166II GTNNAC 6 cut(s) 264, 502, 511, 614, 646, 787
Hpy188I TCNGA 1 cut(s) 194
Hpy188III TCNNGA 5 cut(s) 111, 138, 313, 443, 799
Hpy8I GTNNAC 6 cut(s) 264, 502, 511, 614, 646, 787
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4IV ACGT 2 cut(s) 260, 783
HpyCH4V TGCA 9 cut(s) 264, 278, 366, 422, 527, 620, 695, 755, 778
HpyF10VI GCNNNNNNNGC 4 cut(s) 533, 550, 582, 752
HpyF3I CTNAG 4 cut(s) 191, 311, 393, 838
HpySE526I ACGT 2 cut(s) 260, 783
Hsp92II CATG 4 cut(s) 130, 246, 770, 782
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 6 cut(s) 116, 168, 451, 602, 795, 873
LmnI GCTCC 1 cut(s) 364
Lsp1109I GCAGC 1 cut(s) 531
LweI GCATC 1 cut(s) 438
MaeI CTAG 2 cut(s) 443, 735
MaeII ACGT 2 cut(s) 260, 783
MaeIII GTNAC 2 cut(s) 34, 211
MalI GATC 6 cut(s) 118, 170, 453, 604, 797, 875
MbiI CCGCTC 1 cut(s) 687
MboI GATC 6 cut(s) 116, 168, 451, 602, 795, 873
MboII GAAGA 4 cut(s) 48, 246, 472, 837
MflI RGATCY 1 cut(s) 116
MhlI GDGCHC 2 cut(s) 192, 266
MluCI AATT 6 cut(s) 204, 481, 628, 889, 898, 918
MlyI GAGTC 1 cut(s) 507
MmeI TCCRAC 1 cut(s) 510
MseI TTAA 2 cut(s) 413, 654
MslI CAYNNNNRTG 1 cut(s) 82
MspI CCGG 2 cut(s) 306, 707
MspR9I CCNGG 2 cut(s) 708, 814
Mva1269I GAATGC 1 cut(s) 697
MvaI CCWGG 1 cut(s) 814
MwoI GCNNNNNNNGC 4 cut(s) 533, 550, 582, 752
NciI CCSGG 1 cut(s) 708
NdeII GATC 6 cut(s) 116, 168, 451, 602, 795, 873
NlaIII CATG 4 cut(s) 130, 246, 770, 782
NlaIV GGNNCC 1 cut(s) 705
NspI RCATGY 2 cut(s) 246, 770
OliI CACNNNNGTG 1 cut(s) 82
PciI ACATGT 1 cut(s) 242
PctI GAATGC 1 cut(s) 697
PfeI GAWTC 1 cut(s) 340
PkrI GCNGC 2 cut(s) 428, 546
PleI GAGTC 1 cut(s) 506
PmaCI CACGTG 1 cut(s) 261
PmlI CACGTG 1 cut(s) 261
PpsI GAGTC 1 cut(s) 506
Ppu21I YACGTR 1 cut(s) 261
PscI ACATGT 1 cut(s) 242
PshBI ATTAAT 1 cut(s) 413
Psp6I CCWGG 1 cut(s) 812
PspCI CACGTG 1 cut(s) 261
PspFI CCCAGC 1 cut(s) 581
PspGI CCWGG 1 cut(s) 812
PspN4I GGNNCC 1 cut(s) 705
PspPI GGNCC 1 cut(s) 680
PsuI RGATCY 1 cut(s) 116
RsaI GTAC 2 cut(s) 539, 579
RsaNI GTAC 2 cut(s) 538, 578
RseI CAYNNNNRTG 1 cut(s) 82
SaqAI TTAA 2 cut(s) 413, 654
SatI GCNGC 2 cut(s) 427, 545
Sau3AI GATC 6 cut(s) 116, 168, 451, 602, 795, 873
Sau96I GGNCC 1 cut(s) 680
SchI GAGTC 1 cut(s) 507
ScrFI CCNGG 2 cut(s) 708, 814
SduI GDGCHC 2 cut(s) 192, 266
SfaNI GCATC 1 cut(s) 438
SinI GGWCC 1 cut(s) 680
SmiMI CAYNNNNRTG 1 cut(s) 82
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
Sse9I AATT 6 cut(s) 204, 481, 628, 889, 898, 918
SsiI CCGC 2 cut(s) 426, 687
SspMI CTAG 2 cut(s) 443, 735
StyD4I CCNGG 2 cut(s) 706, 812
TaaI ACNGT 1 cut(s) 436
TaiI ACGT 2 cut(s) 263, 786
TaqI TCGA 3 cut(s) 110, 176, 798
TaqII GACCGA 2 cut(s) 697, 804
TasI AATT 6 cut(s) 204, 481, 628, 889, 898, 918
TauI GCSGC 1 cut(s) 429
TfiI GAWTC 1 cut(s) 340
Tru1I TTAA 2 cut(s) 413, 654
Tru9I TTAA 2 cut(s) 413, 654
TscAI CASTG 1 cut(s) 477
TseI GCWGC 1 cut(s) 544
TspDTI ATGAA 1 cut(s) 143
TspRI CASTG 1 cut(s) 477
VneI GTGCAC 1 cut(s) 262
VpaK11BI GGWCC 1 cut(s) 680
VspI ATTAAT 1 cut(s) 413
XapI RAATTY 4 cut(s) 481, 628, 889, 898
XbaI TCTAGA 1 cut(s) 442
XceI RCATGY 2 cut(s) 246, 770
XmiI GTMKAC 2 cut(s) 501, 786
XspI CTAG 2 cut(s) 443, 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.