Rroxscaffold_3G00264910

Polysaccharide biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014552
Physical Location & Seq
Reverse (-)
58406992 .. 58408807
1816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00264910.1

Sequence Viewer

Length: 1158 bp
ATGTCTCACTTAGTTGCTGAAAAGATCACACTCAACAATATAGATCCTAACCGGCTCTGGGACAAATACTCCAAATATCTAGTAGACTTTCAGCATAACAGCTTAAATTATCATAAACCCCACCACAACCTCTTACAAGAAGGATGTGAAAGCAGGTTGTGGAGCTGTGAAAGCAAACAAAGAGAAACAATGCCCCCAGAAGTGCCTCATTATCGATCTTCTGTAACTCCTCTAGTACGGTTCTCTCCTTCTATCTCACCTGAAGCACAACTAAATCAACTGTACAGACACAAGTATTGGAGAGGAGAGAGAGGAGAGACAAATATGAACTTTGCCAGAAAGAAACTCATCCCTTTTCTTGTTTTAATCCTTTCAATCATTTCTGTACTCAGACTTATTAGAGTAGCAATAACTACATCTTCTTCTTCTTCACATCCATTGCCTGCTTTACCTCCTCTTGGAGACTCATGTTCCAATCCTCCCTCATGCACTAAATTTCAATCAGAGAGAAGTGCCTCCATTAACGCCACACGCCTGCAGAAAAAGGAATACCAACTTCTGTCAAATCTCATTTCCCAGAGAGCCCCTTGCAACCTCCTCATTTTTGGGCTGGAAACTCAGTATCTATCTCTCTCATCAATCAATGCAGGCGGCACTACCATCTTTCTGGAGGATGACGTTAACAAGATAAGCACTATGACAACAGATTCCAACACCACCAGAATCTACAGGGTGGACTACGAGGTACCTGCAAAAGAGGCCTACAACCTGCTGAAGCATGCAAGGGAAAACCCAGTCTGTGCACCTAGCTCAAAACAGCTTCAAGTATCAAAATGTCAGCTTGCGCTGAAAAATTTGCCACAAGAAGTTTATGAGCTTAAATGGGATGTGGTGGTGGTGGATGGACCAAGCGGGAATAGGCCAGAGGCACCAGGCAGGATGGCAGCTATATATACTGCAAGTATGTTCGCAAGAAATGGGAATATTACGGATGTAGTAGTGCATGATGTGGATCGAACAATTGAGAAGTGGTTTTCCTGGGAATTTCTGTGTGATGAGAACTTGGTTTCTTCAAAAGGGAAACTATGGAACTTTAGGATCATGGGTCACACAAATTCTTCAAAATTTTGCCTTCCCAAAGAAGTTACAATAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polysacc_synt_4

Protein Analysis

385

Amino Acids

43.79

Weight (kDa)

8.93

Isoelectric Point (pI)

47.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 183 - 365 2.2e-68 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014552)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24910
fragaria_vesca FvH4_5g08380
malus_domestica MD06G1141300.v1.1 MD14G1156500.v1.1
prunus_persica Prupe.5G150600_v2.0.a1
pyrus_communis pycom06g13130 pycom14g13010
rosa_chinensis RchiOBHm_Chr7g0190071
rosa_laevigata RLG00000004567
rosa_multiflora Rmu_sc0004886.1_g000008
rosa_roxburghii Rroxscaffold_3G00264910
rosa_rugosa Rorug06G0502200
rosa_samantha Rh7AG107800 Rh7BG110100 Rh7CG112300 Rh7DG110700
rosa_wichuraiana Rw7G009300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 144, 757, 777
Acc65I GGTACC 1 cut(s) 745
AccB1I GGYRCC 2 cut(s) 745, 928
AccI GTMKAC 1 cut(s) 84
AciI CCGC 2 cut(s) 651, 912
AclWI GGATC 3 cut(s) 38, 1020, 1106
AcsI RAATTY 5 cut(s) 494, 853, 1043, 1114, 1124
AcuI CTGAAG 2 cut(s) 282, 794
AfaI GTAC 4 cut(s) 237, 284, 387, 747
AfiI CCNNNNNNNGG 2 cut(s) 58, 458
AgsI TTSAA 5 cut(s) 375, 500, 824, 1074, 1122
AjnI CCWGG 2 cut(s) 931, 1037
AluBI AGCT 7 cut(s) 102, 165, 810, 820, 841, 877, 947
AluI AGCT 7 cut(s) 102, 165, 810, 820, 841, 877, 947
Alw21I GWGCWC 1 cut(s) 805
Alw26I GTCTC 3 cut(s) 9, 311, 456
Alw44I GTGCAC 1 cut(s) 801
AlwI GGATC 3 cut(s) 38, 1020, 1106
AoxI GGCC 2 cut(s) 759, 920
ApaLI GTGCAC 1 cut(s) 801
ApeKI GCWGC 1 cut(s) 944
ApoI RAATTY 5 cut(s) 494, 853, 1043, 1114, 1124
Asp718I GGTACC 1 cut(s) 745
AspLEI GCGC 1 cut(s) 847
AspS9I GGNCC 1 cut(s) 905
AsuHPI GGTGA 1 cut(s) 249
AvaII GGWCC 1 cut(s) 905
BaeGI GKGCMC 1 cut(s) 805
BanI GGYRCC 2 cut(s) 745, 928
BanII GRGCYC 1 cut(s) 586
Bbv12I GWGCWC 1 cut(s) 805
BbvI GCAGC 1 cut(s) 956
BccI CCATC 3 cut(s) 668, 896, 934
BcgI CGANNNNNNTGC 2 cut(s) 731, 765
BciT130I CCWGG 2 cut(s) 933, 1039
BcoDI GTCTC 3 cut(s) 9, 311, 456
BfaI CTAG 3 cut(s) 80, 233, 807
BfmI CTRYAG 2 cut(s) 536, 727
BfuAI ACCTGC 3 cut(s) 144, 757, 777
BisI GCNGC 2 cut(s) 652, 945
BlsI GCNGC 2 cut(s) 653, 946
Bme1390I CCNGG 2 cut(s) 933, 1039
Bme18I GGWCC 1 cut(s) 905
BmgT120I GGNCC 1 cut(s) 905
BmiI GGNNCC 2 cut(s) 747, 930
BmrFI CCNGG 2 cut(s) 933, 1039
BmrI ACTGGG 1 cut(s) 788
BmuI ACTGGG 1 cut(s) 788
BpmI CTGGAG 1 cut(s) 689
Bsa29I ATCGAT 1 cut(s) 214
BsaBI GATNNNNATC 1 cut(s) 1011
BsaJI CCNNGG 1 cut(s) 1038
BsaXI ACNNNNNCTCC 2 cut(s) 53, 83
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 458
Bse118I RCCGGY 1 cut(s) 51
Bse1I ACTGG 1 cut(s) 794
Bse3DI GCAATG 1 cut(s) 437
Bse8I GATNNNNATC 1 cut(s) 1011
BseBI CCWGG 2 cut(s) 933, 1039
BseCI ATCGAT 1 cut(s) 214
BseDI CCNNGG 1 cut(s) 1038
BseGI GGATG 8 cut(s) 149, 348, 433, 679, 892, 907, 945, 997
BseJI GATNNNNATC 1 cut(s) 1011
BseLI CCNNNNNNNGG 2 cut(s) 58, 458
BseMI GCAATG 1 cut(s) 437
BseMII CTCAG 2 cut(s) 403, 632
BseNI ACTGG 1 cut(s) 794
BseRI GAGGAG 5 cut(s) 219, 318, 327, 444, 587
BseSI GKGCMC 1 cut(s) 805
BseXI GCAGC 1 cut(s) 956
BshFI GGCC 2 cut(s) 761, 922
BshNI GGYRCC 2 cut(s) 745, 928
BshVI ATCGAT 1 cut(s) 214
BsiHKAI GWGCWC 1 cut(s) 805
BsiSI CCGG 1 cut(s) 52
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 2 cut(s) 58, 458
BsmAI GTCTC 3 cut(s) 9, 311, 456
BsmFI GGGAC 1 cut(s) 74
BsnI GGCC 2 cut(s) 761, 922
Bsp1286I GDGCHC 2 cut(s) 586, 805
Bsp1407I TGTACA 1 cut(s) 282
Bsp143I GATC 5 cut(s) 24, 43, 215, 1012, 1098
BspACI CCGC 2 cut(s) 651, 912
BspANI GGCC 2 cut(s) 761, 922
BspCNI CTCAG 2 cut(s) 402, 631
BspDI ATCGAT 1 cut(s) 214
BspLI GGNNCC 2 cut(s) 747, 930
BspMAI CTGCAG 1 cut(s) 540
BspMI ACCTGC 3 cut(s) 144, 757, 777
BspPI GGATC 3 cut(s) 38, 1020, 1106
BspT107I GGYRCC 2 cut(s) 745, 928
BsrDI GCAATG 1 cut(s) 437
BsrFI RCCGGY 1 cut(s) 51
BsrGI TGTACA 1 cut(s) 282
BsrI ACTGG 1 cut(s) 794
BssAI RCCGGY 1 cut(s) 51
BssECI CCNNGG 1 cut(s) 1038
BssMI GATC 5 cut(s) 24, 43, 215, 1012, 1098
Bst2UI CCWGG 2 cut(s) 933, 1039
Bst4CI ACNGT 2 cut(s) 240, 282
BstAUI TGTACA 1 cut(s) 282
BstC8I GCNNGC 5 cut(s) 444, 536, 649, 780, 843
BstDEI CTNAG 3 cut(s) 10, 389, 618
BstF5I GGATG 8 cut(s) 149, 348, 433, 679, 892, 907, 945, 997
BstHHI GCGC 1 cut(s) 847
BstKTI GATC 5 cut(s) 27, 46, 218, 1015, 1101
BstMAI GTCTC 3 cut(s) 9, 311, 456
BstMBI GATC 5 cut(s) 24, 43, 215, 1012, 1098
BstMWI GCNNNNNNNGC 2 cut(s) 171, 758
BstNI CCWGG 2 cut(s) 933, 1039
BstNSI RCATGY 1 cut(s) 782
BstSCI CCNGG 2 cut(s) 931, 1037
BstSFI CTRYAG 2 cut(s) 536, 727
BstSLI GKGCMC 1 cut(s) 805
BstV1I GCAGC 1 cut(s) 956
BstX2I RGATCY 1 cut(s) 43
BstXI CCANNNNNNTGG 1 cut(s) 667
BstYI RGATCY 1 cut(s) 43
Bsu15I ATCGAT 1 cut(s) 214
BsuRI GGCC 2 cut(s) 761, 922
BsuTUI ATCGAT 1 cut(s) 214
BtsCI GGATG 8 cut(s) 149, 348, 433, 679, 892, 907, 945, 997
BveI ACCTGC 3 cut(s) 144, 757, 777
Cac8I GCNNGC 5 cut(s) 444, 536, 649, 780, 843
CfoI GCGC 1 cut(s) 847
Cfr10I RCCGGY 1 cut(s) 51
Cfr13I GGNCC 1 cut(s) 905
ClaI ATCGAT 1 cut(s) 214
Csp6I GTAC 4 cut(s) 236, 283, 386, 746
CviAII CATG 5 cut(s) 468, 486, 779, 1004, 1102
CviQI GTAC 4 cut(s) 236, 283, 386, 746
DdeI CTNAG 3 cut(s) 10, 389, 618
DpnI GATC 5 cut(s) 26, 45, 217, 1014, 1100
DpnII GATC 5 cut(s) 24, 43, 215, 1012, 1098
Eco147I AGGCCT 1 cut(s) 761
Eco24I GRGCYC 1 cut(s) 586
Eco47I GGWCC 1 cut(s) 905
Eco57I CTGAAG 2 cut(s) 282, 794
EcoRII CCWGG 2 cut(s) 931, 1037
EcoT38I GRGCYC 1 cut(s) 586
FaeI CATG 5 cut(s) 471, 489, 782, 1007, 1105
FaqI GGGAC 1 cut(s) 74
FatI CATG 5 cut(s) 467, 485, 778, 1003, 1101
FauI CCCGC 1 cut(s) 905
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 2 cut(s) 652, 945
FokI GGATG 8 cut(s) 156, 335, 420, 686, 899, 914, 952, 1004
FriOI GRGCYC 1 cut(s) 586
Fsp4HI GCNGC 2 cut(s) 652, 945
FspBI CTAG 3 cut(s) 80, 233, 807
GlaI GCGC 1 cut(s) 846
GluI GCNGC 2 cut(s) 652, 945
GsuI CTGGAG 1 cut(s) 689
HaeIII GGCC 2 cut(s) 761, 922
HapII CCGG 1 cut(s) 52
HhaI GCGC 1 cut(s) 847
Hin1II CATG 5 cut(s) 471, 489, 782, 1007, 1105
Hin6I GCGC 1 cut(s) 845
HinP1I GCGC 1 cut(s) 845
HincII GTYRAC 1 cut(s) 682
HindII GTYRAC 1 cut(s) 682
HinfI GANTC 3 cut(s) 464, 707, 723
HpaI GTTAAC 1 cut(s) 682
HpaII CCGG 1 cut(s) 52
HphI GGTGA 1 cut(s) 249
Hpy166II GTNNAC 4 cut(s) 85, 682, 736, 803
Hpy188I TCNGA 2 cut(s) 392, 505
Hpy188III TCNNGA 1 cut(s) 668
Hpy8I GTNNAC 4 cut(s) 85, 682, 736, 803
HpyAV CCTTC 3 cut(s) 134, 258, 1142
HpyCH4III ACNGT 2 cut(s) 240, 282
HpyCH4IV ACGT 1 cut(s) 678
HpyCH4V TGCA 9 cut(s) 489, 538, 591, 647, 752, 782, 803, 959, 1003
HpyF10VI GCNNNNNNNGC 2 cut(s) 171, 758
HpyF3I CTNAG 3 cut(s) 10, 389, 618
HpySE526I ACGT 1 cut(s) 678
Hsp92II CATG 5 cut(s) 471, 489, 782, 1007, 1105
HspAI GCGC 1 cut(s) 845
KpnI GGTACC 1 cut(s) 749
KspAI GTTAAC 1 cut(s) 682
Kzo9I GATC 5 cut(s) 24, 43, 215, 1012, 1098
LmnI GCTCC 1 cut(s) 162
Lsp1109I GCAGC 1 cut(s) 956
MaeI CTAG 3 cut(s) 80, 233, 807
MaeII ACGT 1 cut(s) 678
MaeIII GTNAC 3 cut(s) 223, 1106, 1144
MalI GATC 5 cut(s) 26, 45, 217, 1014, 1100
MboI GATC 5 cut(s) 24, 43, 215, 1012, 1098
MboII GAAGA 7 cut(s) 210, 411, 414, 417, 420, 1062, 1110
MfeI CAATTG 1 cut(s) 1020
MflI RGATCY 1 cut(s) 43
MhlI GDGCHC 2 cut(s) 586, 805
MluCI AATT 7 cut(s) 106, 494, 853, 1020, 1043, 1114, 1124
MlyI GAGTC 1 cut(s) 458
MmeI TCCRAC 1 cut(s) 735
MseI TTAA 5 cut(s) 104, 365, 522, 681, 879
MspI CCGG 1 cut(s) 52
MspR9I CCNGG 2 cut(s) 933, 1039
MunI CAATTG 1 cut(s) 1020
MvaI CCWGG 2 cut(s) 933, 1039
MwoI GCNNNNNNNGC 2 cut(s) 171, 758
NdeII GATC 5 cut(s) 24, 43, 215, 1012, 1098
NlaIII CATG 5 cut(s) 471, 489, 782, 1007, 1105
NlaIV GGNNCC 2 cut(s) 747, 930
NmuCI GTSAC 1 cut(s) 1106
NspI RCATGY 1 cut(s) 782
PaeI GCATGC 1 cut(s) 782
PceI AGGCCT 1 cut(s) 761
PfeI GAWTC 2 cut(s) 707, 723
PkrI GCNGC 2 cut(s) 653, 946
PleI GAGTC 1 cut(s) 458
PpsI GAGTC 1 cut(s) 458
Psp6I CCWGG 2 cut(s) 931, 1037
PspGI CCWGG 2 cut(s) 931, 1037
PspN4I GGNNCC 2 cut(s) 747, 930
PspPI GGNCC 1 cut(s) 905
PstI CTGCAG 1 cut(s) 540
PsuI RGATCY 1 cut(s) 43
RsaI GTAC 4 cut(s) 237, 284, 387, 747
RsaNI GTAC 4 cut(s) 236, 283, 386, 746
SaqAI TTAA 5 cut(s) 104, 365, 522, 681, 879
SatI GCNGC 2 cut(s) 652, 945
Sau3AI GATC 5 cut(s) 24, 43, 215, 1012, 1098
Sau96I GGNCC 1 cut(s) 905
SchI GAGTC 1 cut(s) 458
ScrFI CCNGG 2 cut(s) 933, 1039
SduI GDGCHC 2 cut(s) 586, 805
SfcI CTRYAG 2 cut(s) 536, 727
SinI GGWCC 1 cut(s) 905
SphI GCATGC 1 cut(s) 782
Sse9I AATT 7 cut(s) 106, 494, 853, 1020, 1043, 1114, 1124
SseBI AGGCCT 1 cut(s) 761
SsiI CCGC 2 cut(s) 651, 912
SspI AATATT 1 cut(s) 985
SspMI CTAG 3 cut(s) 80, 233, 807
StuI AGGCCT 1 cut(s) 761
StyD4I CCNGG 2 cut(s) 931, 1037
TaaI ACNGT 2 cut(s) 240, 282
TaiI ACGT 1 cut(s) 681
TaqI TCGA 2 cut(s) 214, 1015
TasI AATT 7 cut(s) 106, 494, 853, 1020, 1043, 1114, 1124
TatI WGTACW 2 cut(s) 282, 385
TauI GCSGC 1 cut(s) 654
TfiI GAWTC 2 cut(s) 707, 723
Tru1I TTAA 5 cut(s) 104, 365, 522, 681, 879
Tru9I TTAA 5 cut(s) 104, 365, 522, 681, 879
TseFI GTSAC 1 cut(s) 1106
TseI GCWGC 1 cut(s) 944
Tsp45I GTSAC 1 cut(s) 1106
TspDTI ATGAA 1 cut(s) 341
TspGWI ACGGA 1 cut(s) 1004
VneI GTGCAC 1 cut(s) 801
VpaK11BI GGWCC 1 cut(s) 905
XapI RAATTY 5 cut(s) 494, 853, 1043, 1114, 1124
XceI RCATGY 1 cut(s) 782
XmiI GTMKAC 1 cut(s) 84
XspI CTAG 3 cut(s) 80, 233, 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.