FvH4_5g14080

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006410
Physical Location & Seq
Reverse (-)
7970192 .. 7971908
1717 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14080.t1

Sequence Viewer

Length: 543 bp
ATGGAAGCCCAGAAAGGAGAAGGAGAAGTGGTGATGAGTTCTGATTTGCTGCTTCCAGTTGGGTTTAGGTTTATGCCAACAGATGAAGAGCTCGTCAGATACTATCTAATGAACAAAGCTTGTTTTAGAGCTGTACCAGTTGCTGATGCGATTCAGGAGATCGATGCGACTCGGTTTTACAGCAACCATCCGAAGAATCTAGTGACGTTCTCTAATGGGGAGAGGGAGTGGTTCTTCTTTATACACGAAGACGATGAAAATTACAGTGGCAGCTGCTGCAGCCATGCCCAGAGAAGAAGAAATGTTCGAGAAGTCGGAAACGGGATAGGGTTTTGGAAACCAAGCGGATCAGAAAACCCGATCTACAGTAAAGATGGAAATGTCTTCGCCACCAAGATCTTCCTCACTTACTTCTCCGGAAGTTGTCACTCCAAAAAACCAAAGAGAACTCACTGGAAAATAGTCGAGTACCATTTACAGATAAGAAGAGAGTGGGTGGTGCTGGGAAAATTACAGAGAGGAATGGATTACACTGGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

181

Amino Acids

20.92

Weight (kDa)

8.32

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 19 - 159 5.4e-25 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 416
AciI CCGC 1 cut(s) 345
AclWI GGATC 1 cut(s) 355
AfaI GTAC 2 cut(s) 135, 470
AluBI AGCT 4 cut(s) 91, 119, 131, 273
AluI AGCT 4 cut(s) 91, 119, 131, 273
Alw21I GWGCWC 1 cut(s) 93
AlwI GGATC 1 cut(s) 355
AlwNI CAGNNNCTG 2 cut(s) 143, 276
Aor13HI TCCGGA 1 cut(s) 416
ApeKI GCWGC 5 cut(s) 49, 270, 273, 276, 279
AsuHPI GGTGA 1 cut(s) 43
BanII GRGCYC 1 cut(s) 93
BbsI GAAGAC 2 cut(s) 255, 376
Bbv12I GWGCWC 1 cut(s) 93
BbvI GCAGC 5 cut(s) 36, 260, 263, 282, 291
BccI CCATC 2 cut(s) 195, 368
BfaI CTAG 1 cut(s) 200
BfmI CTRYAG 2 cut(s) 277, 364
BglII AGATCT 1 cut(s) 396
BisI GCNGC 5 cut(s) 50, 271, 274, 277, 280
BlsI GCNGC 5 cut(s) 51, 272, 275, 278, 281
BmsI GCATC 2 cut(s) 136, 154
BpiI GAAGAC 2 cut(s) 255, 376
Bsa29I ATCGAT 1 cut(s) 162
BsaWI WCCGGW 1 cut(s) 416
BsaXI ACNNNNNCTCC 4 cut(s) 15, 45, 212, 242
Bse1I ACTGG 4 cut(s) 56, 137, 458, 538
BseAI TCCGGA 1 cut(s) 416
BseCI ATCGAT 1 cut(s) 162
BseGI GGATG 1 cut(s) 187
BseNI ACTGG 4 cut(s) 56, 137, 458, 538
BseXI GCAGC 5 cut(s) 36, 260, 263, 282, 291
BseYI CCCAGC 1 cut(s) 502
BshVI ATCGAT 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 93
BsiSI CCGG 1 cut(s) 417
Bsp1286I GDGCHC 1 cut(s) 93
Bsp13I TCCGGA 1 cut(s) 416
Bsp143I GATC 4 cut(s) 159, 347, 360, 396
BspACI CCGC 1 cut(s) 345
BspDI ATCGAT 1 cut(s) 162
BspEI TCCGGA 1 cut(s) 416
BspMAI CTGCAG 1 cut(s) 281
BspPI GGATC 1 cut(s) 355
BspQI GCTCTTC 1 cut(s) 81
BsrI ACTGG 4 cut(s) 56, 137, 458, 538
BssMI GATC 4 cut(s) 159, 347, 360, 396
Bst4CI ACNGT 2 cut(s) 266, 368
Bst6I CTCTTC 2 cut(s) 81, 481
BstAPI GCANNNNNTGC 1 cut(s) 276
BstF5I GGATG 1 cut(s) 187
BstKTI GATC 4 cut(s) 162, 350, 363, 399
BstMBI GATC 4 cut(s) 159, 347, 360, 396
BstMWI GCNNNNNNNGC 2 cut(s) 276, 279
BstSFI CTRYAG 2 cut(s) 277, 364
BstV1I GCAGC 5 cut(s) 36, 260, 263, 282, 291
BstV2I GAAGAC 2 cut(s) 255, 376
BstX2I RGATCY 1 cut(s) 396
BstYI RGATCY 1 cut(s) 396
Bsu15I ATCGAT 1 cut(s) 162
BsuTUI ATCGAT 1 cut(s) 162
BtsCI GGATG 1 cut(s) 187
BtsIMutI CAGTG 3 cut(s) 271, 451, 531
CaiI CAGNNNCTG 2 cut(s) 143, 276
ClaI ATCGAT 1 cut(s) 162
Csp6I GTAC 2 cut(s) 134, 469
CviAII CATG 1 cut(s) 284
CviJI RGCY 6 cut(s) 8, 91, 119, 131, 273, 282
CviKI_1 RGCY 6 cut(s) 8, 91, 119, 131, 273, 282
CviQI GTAC 2 cut(s) 134, 469
DpnI GATC 4 cut(s) 161, 349, 362, 398
DpnII GATC 4 cut(s) 159, 347, 360, 396
Eam1104I CTCTTC 2 cut(s) 81, 481
EarI CTCTTC 2 cut(s) 81, 481
Ecl136II GAGCTC 1 cut(s) 91
Eco24I GRGCYC 1 cut(s) 93
Eco53kI GAGCTC 1 cut(s) 91
EcoICRI GAGCTC 1 cut(s) 91
EcoT38I GRGCYC 1 cut(s) 93
FaeI CATG 1 cut(s) 287
FaiI YATR 3 cut(s) 74, 242, 285
FatI CATG 1 cut(s) 283
Fnu4HI GCNGC 5 cut(s) 50, 271, 274, 277, 280
FokI GGATG 1 cut(s) 174
FriOI GRGCYC 1 cut(s) 93
Fsp4HI GCNGC 5 cut(s) 50, 271, 274, 277, 280
FspBI CTAG 1 cut(s) 200
GluI GCNGC 5 cut(s) 50, 271, 274, 277, 280
GsaI CCCAGC 1 cut(s) 506
HapII CCGG 1 cut(s) 417
Hin1II CATG 1 cut(s) 287
HindIII AAGCTT 1 cut(s) 117
HinfI GANTC 3 cut(s) 151, 169, 196
HpaII CCGG 1 cut(s) 417
HphI GGTGA 1 cut(s) 43
Hpy188I TCNGA 5 cut(s) 43, 98, 192, 317, 352
Hpy188III TCNNGA 3 cut(s) 155, 308, 417
HpyAV CCTTC 1 cut(s) 14
HpyCH4III ACNGT 2 cut(s) 266, 368
HpyCH4IV ACGT 1 cut(s) 206
HpyCH4V TGCA 1 cut(s) 279
HpyF10VI GCNNNNNNNGC 2 cut(s) 276, 279
HpySE526I ACGT 1 cut(s) 206
Hsp92II CATG 1 cut(s) 287
Kpn2I TCCGGA 1 cut(s) 416
Kzo9I GATC 4 cut(s) 159, 347, 360, 396
LguI GCTCTTC 1 cut(s) 81
LpnPI CCDG 9 cut(s) 23, 69, 140, 150, 302, 430, 439, 488, 519
Lsp1109I GCAGC 5 cut(s) 36, 260, 263, 282, 291
LweI GCATC 2 cut(s) 136, 154
MaeI CTAG 1 cut(s) 200
MaeII ACGT 1 cut(s) 206
MaeIII GTNAC 2 cut(s) 202, 425
MalI GATC 4 cut(s) 161, 349, 362, 398
MboI GATC 4 cut(s) 159, 347, 360, 396
MboII GAAGA 9 cut(s) 98, 205, 226, 260, 306, 309, 376, 391, 498
MflI RGATCY 1 cut(s) 396
MhlI GDGCHC 1 cut(s) 93
MluCI AATT 2 cut(s) 259, 509
MlyI GAGTC 1 cut(s) 163
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 3 cut(s) 216, 413, 512
MroI TCCGGA 1 cut(s) 416
MspA1I CMGCKG 1 cut(s) 273
MspI CCGG 1 cut(s) 417
MwoI GCNNNNNNNGC 2 cut(s) 276, 279
NdeII GATC 4 cut(s) 159, 347, 360, 396
NlaIII CATG 1 cut(s) 287
NmuCI GTSAC 2 cut(s) 202, 425
PciSI GCTCTTC 1 cut(s) 81
PfeI GAWTC 2 cut(s) 151, 196
PkrI GCNGC 5 cut(s) 51, 272, 275, 278, 281
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
Psp124BI GAGCTC 1 cut(s) 93
PspFI CCCAGC 1 cut(s) 502
PstI CTGCAG 1 cut(s) 281
PstNI CAGNNNCTG 2 cut(s) 143, 276
PsuI RGATCY 1 cut(s) 396
PvuII CAGCTG 1 cut(s) 273
RsaI GTAC 2 cut(s) 135, 470
RsaNI GTAC 2 cut(s) 134, 469
SacI GAGCTC 1 cut(s) 93
SapI GCTCTTC 1 cut(s) 81
SatI GCNGC 5 cut(s) 50, 271, 274, 277, 280
Sau3AI GATC 4 cut(s) 159, 347, 360, 396
SchI GAGTC 1 cut(s) 163
SduI GDGCHC 1 cut(s) 93
SetI ASST 6 cut(s) 71, 93, 121, 133, 209, 275
SfaNI GCATC 2 cut(s) 136, 154
SfcI CTRYAG 2 cut(s) 277, 364
Sse9I AATT 2 cut(s) 259, 509
SsiI CCGC 1 cut(s) 345
SspMI CTAG 1 cut(s) 200
SstI GAGCTC 1 cut(s) 93
TaaI ACNGT 2 cut(s) 266, 368
TaiI ACGT 1 cut(s) 209
TaqI TCGA 3 cut(s) 162, 307, 465
TasI AATT 2 cut(s) 259, 509
TfiI GAWTC 2 cut(s) 151, 196
TscAI CASTG 3 cut(s) 271, 458, 538
TseFI GTSAC 2 cut(s) 202, 425
TseI GCWGC 5 cut(s) 49, 270, 273, 276, 279
Tsp45I GTSAC 2 cut(s) 202, 425
TspDTI ATGAA 3 cut(s) 99, 125, 270
TspRI CASTG 3 cut(s) 271, 458, 538
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.