MD14G1210000.v1.1

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006410
Physical Location & Seq
Forward (+)
29583803 .. 29585316
1514 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1210000.v1.1.491

Sequence Viewer

Length: 525 bp
ATGATCATCGGCTTGAATCAGCTGCCTGTCGGGTTTAGGTTTATGCCAACAGACGAAGAGTTGGTCACTCATTATCTGATGAACAAGGTGTTGTATCGGCCTGTACCAGCTGCTCAGGAAATTCGGGAGATCGATGCGGCTCGATTCTACAGCAACCATCCTAAGAATCTAGTGACGTTCTCCTGCGGGGAGAGAGAGTGGTTTTTCTTCATCCATCAAGGTGAAAGCAGTTATGCATCCCAAACCAGAAGCAGTATTCGAGTAGTTGGAAATGGTTTAGGGTTTTGGAAACCCAAGTACGGATCAGAAACTCCCATCTGCGATGAGGATGGACATTTCTTTGCCTCCAAGATCTCTCTCACTTACTTCTCGGGAAGAAGTGCTTCGCATGCAAAGAGAACACATTGGAAAATGGTGGAGTACTACTTACATGAACATGATCACGTCGAGTCTAACACAGAAGAAAAGTACCAAGGTAAAAGACGAAATACAACCATACTCTACTTTCATGGGGCACTGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

175

Amino Acids

20.29

Weight (kDa)

7.85

Isoelectric Point (pI)

52.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 9 - 142 1.1e-24 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 137, 186
AclWI GGATC 1 cut(s) 310
AcsI RAATTY 1 cut(s) 120
AfaI GTAC 4 cut(s) 105, 299, 422, 470
AfiI CCNNNNNNNGG 1 cut(s) 299
AgsI TTSAA 1 cut(s) 16
AjiI CACGTC 1 cut(s) 445
AluBI AGCT 2 cut(s) 22, 110
AluI AGCT 2 cut(s) 22, 110
AlwI GGATC 1 cut(s) 310
Ama87I CYCGRG 1 cut(s) 370
AoxI GGCC 1 cut(s) 98
ApeKI GCWGC 2 cut(s) 22, 110
ApoI RAATTY 1 cut(s) 120
ArsI GACNNNNNNTTYG 2 cut(s) 48, 80
Asp700I GAANNNNTTC 1 cut(s) 382
AsuHPI GGTGA 1 cut(s) 233
AvaI CYCGRG 1 cut(s) 370
BaeGI GKGCMC 1 cut(s) 517
BbvI GCAGC 2 cut(s) 9, 97
BccI CCATC 4 cut(s) 165, 222, 323, 323
BclI TGATCA 2 cut(s) 3, 439
BfaI CTAG 1 cut(s) 170
BfmI CTRYAG 1 cut(s) 148
BglII AGATCT 1 cut(s) 351
BisI GCNGC 3 cut(s) 23, 111, 138
BlsI GCNGC 3 cut(s) 24, 112, 139
BmcAI AGTACT 1 cut(s) 422
BmeT110I CYCGRG 1 cut(s) 370
BmgBI CACGTC 1 cut(s) 445
BmsI GCATC 2 cut(s) 124, 245
Bpu10I CCTNAGC 1 cut(s) 114
Bsa29I ATCGAT 1 cut(s) 132
BsaJI CCNNGG 1 cut(s) 472
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 1 cut(s) 299
BseCI ATCGAT 1 cut(s) 132
BseDI CCNNGG 1 cut(s) 472
BseGI GGATG 4 cut(s) 157, 210, 236, 334
BseLI CCNNNNNNNGG 1 cut(s) 299
BseMII CTCAG 1 cut(s) 128
BseSI GKGCMC 1 cut(s) 517
BseXI GCAGC 2 cut(s) 9, 97
BshFI GGCC 1 cut(s) 100
BshVI ATCGAT 1 cut(s) 132
BsiHKCI CYCGRG 1 cut(s) 370
BslI CCNNNNNNNGG 1 cut(s) 299
BsnI GGCC 1 cut(s) 100
BsoBI CYCGRG 1 cut(s) 370
Bsp1286I GDGCHC 1 cut(s) 517
Bsp143I GATC 5 cut(s) 3, 129, 302, 351, 439
BspACI CCGC 2 cut(s) 137, 186
BspANI GGCC 1 cut(s) 100
BspCNI CTCAG 1 cut(s) 127
BspDI ATCGAT 1 cut(s) 132
BspPI GGATC 1 cut(s) 310
BssECI CCNNGG 1 cut(s) 472
BssMI GATC 5 cut(s) 3, 129, 302, 351, 439
BssT1I CCWWGG 1 cut(s) 472
Bst6I CTCTTC 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 390
BstDEI CTNAG 2 cut(s) 114, 162
BstF5I GGATG 4 cut(s) 157, 210, 236, 334
BstKTI GATC 5 cut(s) 6, 132, 305, 354, 442
BstMBI GATC 5 cut(s) 3, 129, 302, 351, 439
BstMWI GCNNNNNNNGC 1 cut(s) 389
BstNSI RCATGY 1 cut(s) 392
BstSFI CTRYAG 1 cut(s) 148
BstSLI GKGCMC 1 cut(s) 517
BstV1I GCAGC 2 cut(s) 9, 97
BstX2I RGATCY 1 cut(s) 351
BstYI RGATCY 1 cut(s) 351
Bsu15I ATCGAT 1 cut(s) 132
BsuRI GGCC 1 cut(s) 100
BsuTUI ATCGAT 1 cut(s) 132
BtgZI GCGATG 1 cut(s) 336
BtrI CACGTC 1 cut(s) 445
BtsCI GGATG 4 cut(s) 157, 210, 236, 334
BtsI GCAGTG 1 cut(s) 515
BtsIMutI CAGTG 1 cut(s) 515
Cac8I GCNNGC 1 cut(s) 390
ClaI ATCGAT 1 cut(s) 132
Csp6I GTAC 4 cut(s) 104, 298, 421, 469
CviAII CATG 4 cut(s) 389, 431, 437, 509
CviJI RGCY 5 cut(s) 12, 22, 100, 110, 140
CviKI_1 RGCY 5 cut(s) 12, 22, 100, 110, 140
CviQI GTAC 4 cut(s) 104, 298, 421, 469
DdeI CTNAG 2 cut(s) 114, 162
DpnI GATC 5 cut(s) 5, 131, 304, 353, 441
DpnII GATC 5 cut(s) 3, 129, 302, 351, 439
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 472
Eco88I CYCGRG 1 cut(s) 370
EcoT14I CCWWGG 1 cut(s) 472
EcoT22I ATGCAT 1 cut(s) 238
ErhI CCWWGG 1 cut(s) 472
FaeI CATG 4 cut(s) 392, 434, 440, 512
FaiI YATR 7 cut(s) 44, 234, 390, 432, 438, 497, 510
FalI AAGNNNNNCTT 2 cut(s) 367, 399
FatI CATG 4 cut(s) 388, 430, 436, 508
FauI CCCGC 1 cut(s) 179
FbaI TGATCA 2 cut(s) 3, 439
Fnu4HI GCNGC 3 cut(s) 23, 111, 138
FokI GGATG 4 cut(s) 144, 197, 223, 341
Fsp4HI GCNGC 3 cut(s) 23, 111, 138
FspBI CTAG 1 cut(s) 170
GluI GCNGC 3 cut(s) 23, 111, 138
HaeIII GGCC 1 cut(s) 100
Hin1II CATG 4 cut(s) 392, 434, 440, 512
HinfI GANTC 4 cut(s) 16, 144, 166, 449
HphI GGTGA 1 cut(s) 233
Hpy188I TCNGA 2 cut(s) 78, 307
Hpy188III TCNNGA 3 cut(s) 116, 125, 372
Hpy99I CGWCG 1 cut(s) 449
HpyCH4IV ACGT 2 cut(s) 176, 444
HpyCH4V TGCA 3 cut(s) 236, 392, 520
HpyF10VI GCNNNNNNNGC 1 cut(s) 389
HpyF3I CTNAG 2 cut(s) 114, 162
HpySE526I ACGT 2 cut(s) 176, 444
Hsp92II CATG 4 cut(s) 392, 434, 440, 512
Ksp22I TGATCA 2 cut(s) 3, 439
Kzo9I GATC 5 cut(s) 3, 129, 302, 351, 439
LpnPI CCDG 6 cut(s) 39, 101, 114, 120, 196, 259
Lsp1109I GCAGC 2 cut(s) 9, 97
LweI GCATC 2 cut(s) 124, 245
MaeI CTAG 1 cut(s) 170
MaeII ACGT 2 cut(s) 176, 444
MaeIII GTNAC 2 cut(s) 64, 172
MalI GATC 5 cut(s) 5, 131, 304, 353, 441
MboI GATC 5 cut(s) 3, 129, 302, 351, 439
MboII GAAGA 4 cut(s) 68, 199, 387, 473
MflI RGATCY 1 cut(s) 351
MhlI GDGCHC 1 cut(s) 517
MluCI AATT 1 cut(s) 120
MlyI GAGTC 1 cut(s) 458
MmeI TCCRAC 1 cut(s) 247
MnlI CCTC 2 cut(s) 319, 355
Mph1103I ATGCAT 1 cut(s) 238
MroXI GAANNNNTTC 1 cut(s) 382
MslI CAYNNNNRTG 3 cut(s) 219, 435, 520
MspA1I CMGCKG 2 cut(s) 22, 110
MwoI GCNNNNNNNGC 1 cut(s) 389
NdeII GATC 5 cut(s) 3, 129, 302, 351, 439
NlaIII CATG 4 cut(s) 392, 434, 440, 512
NmuCI GTSAC 2 cut(s) 64, 172
NsiI ATGCAT 1 cut(s) 238
NspI RCATGY 1 cut(s) 392
PaeI GCATGC 1 cut(s) 392
PdmI GAANNNNTTC 1 cut(s) 382
PfeI GAWTC 3 cut(s) 16, 144, 166
PkrI GCNGC 3 cut(s) 24, 112, 139
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
PsuI RGATCY 1 cut(s) 351
PvuII CAGCTG 2 cut(s) 22, 110
RsaI GTAC 4 cut(s) 105, 299, 422, 470
RsaNI GTAC 4 cut(s) 104, 298, 421, 469
RseI CAYNNNNRTG 3 cut(s) 219, 435, 520
SatI GCNGC 3 cut(s) 23, 111, 138
Sau3AI GATC 5 cut(s) 3, 129, 302, 351, 439
ScaI AGTACT 1 cut(s) 422
SchI GAGTC 1 cut(s) 458
SduI GDGCHC 1 cut(s) 517
SetI ASST 8 cut(s) 24, 41, 90, 112, 179, 223, 447, 478
SfaNI GCATC 2 cut(s) 124, 245
SfcI CTRYAG 1 cut(s) 148
SmiMI CAYNNNNRTG 3 cut(s) 219, 435, 520
SphI GCATGC 1 cut(s) 392
Sse9I AATT 1 cut(s) 120
SsiI CCGC 2 cut(s) 137, 186
SspMI CTAG 1 cut(s) 170
StyI CCWWGG 1 cut(s) 472
TaiI ACGT 2 cut(s) 179, 447
TaqI TCGA 4 cut(s) 132, 142, 259, 447
TasI AATT 1 cut(s) 120
TatI WGTACW 1 cut(s) 420
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 3 cut(s) 16, 144, 166
TscAI CASTG 1 cut(s) 522
TseFI GTSAC 2 cut(s) 64, 172
TseI GCWGC 2 cut(s) 22, 110
Tsp45I GTSAC 2 cut(s) 64, 172
TspDTI ATGAA 4 cut(s) 95, 199, 447, 497
TspGWI ACGGA 1 cut(s) 315
TspRI CASTG 1 cut(s) 522
XapI RAATTY 1 cut(s) 120
XceI RCATGY 1 cut(s) 392
XmnI GAANNNNTTC 1 cut(s) 382
XspI CTAG 1 cut(s) 170
ZrmI AGTACT 1 cut(s) 422
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.