Rroxscaffold_5G00344500

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006410
Physical Location & Seq
Forward (+)
14597393 .. 14600847
3455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00344500.1

Sequence Viewer

Length: 756 bp
ATGAGACATATTTTGGGCCCCAACCCCAACATTATTCCATATGGATCCAACATCCAAGCATGCCAGAAGTTCATGGGTTCTTTGTTACAAACCAGTTATTGTTCTCTATTTACCATATCTTCTTCTTCTTCTTCTTTGGCGACTTCGATCTACTCCGCTCCGAGCTTCAAGCTCGGGAGGCCATCAAGAAAAGAGTTTTTGCGAGTGAAGGAGTTTCTCGGCTTCCTTGGTTCCCAAGTGACGTTCTCTGATGGAGAGAGGGAGTGGTTCTTCTTTATTCATGAAGACGATGAAAGTTGCAGCAGCCTTGCCCAGAGAAAAAGAACTGTTCGAGAAGTCGGAAACGGGTTAGGGTTTTGGAAACGGAACGGATCAGAAAACCCGATCTACAATGAAGATGGAAACGTTTTTGCCACCAAGATCTACCTCACTTACTTCTCCGGAAGTTGTCACTCCAATAAACCAAAGAGAACCCACTGGAAAATGGTCGAGTACCATTTACAGATAAGAAGAGAATGGGTAGTGCTGGGAAGATTACAAAGAGGAATGGATTACACTGCTGCGACATTGCCGCATGATATGCATGTTGCTGCAATAACATACAATTGGTGCCCCGCCTCAGAAACACCTTCGAACCTTTGCATGCCGAGGTTCATGAAGATTGCCGAGTACAAGGCCGAGATTGTCCCCATATTACAGGAAATCCATATTGGCTTCTTGAACTGCTGTACAATCAACAATGTAGGAGGGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

251

Amino Acids

28.52

Weight (kDa)

8.88

Isoelectric Point (pI)

58.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 84 - 167 2.3e-11 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 609
AccBSI CCGCTC 1 cut(s) 158
AccIII TCCGGA 1 cut(s) 440
AciI CCGC 3 cut(s) 156, 572, 615
AclI AACGTT 1 cut(s) 405
AclWI GGATC 3 cut(s) 39, 52, 379
AfaI GTAC 3 cut(s) 494, 671, 730
AgsI TTSAA 2 cut(s) 169, 721
AjuI GAANNNNNNNTTGG 2 cut(s) 693, 725
AluBI AGCT 2 cut(s) 165, 172
AluI AGCT 2 cut(s) 165, 172
AlwI GGATC 3 cut(s) 39, 52, 379
Ama87I CYCGRG 1 cut(s) 173
Aor13HI TCCGGA 1 cut(s) 440
AoxI GGCC 3 cut(s) 16, 179, 675
ApaI GGGCCC 1 cut(s) 20
ApeKI GCWGC 4 cut(s) 300, 303, 560, 590
AspS9I GGNCC 2 cut(s) 16, 17
AsuII TTCGAA 1 cut(s) 632
AvaI CYCGRG 1 cut(s) 173
BaeGI GKGCMC 2 cut(s) 20, 614
BamHI GGATCC 1 cut(s) 44
BanI GGYRCC 1 cut(s) 609
BanII GRGCYC 1 cut(s) 20
BbsI GAAGAC 1 cut(s) 291
BbvI GCAGC 4 cut(s) 312, 315, 547, 577
BccI CCATC 3 cut(s) 190, 245, 392
BglII AGATCT 1 cut(s) 420
BisI GCNGC 5 cut(s) 301, 304, 561, 572, 591
BlsI GCNGC 5 cut(s) 302, 305, 562, 573, 592
BmeT110I CYCGRG 1 cut(s) 173
BmgT120I GGNCC 2 cut(s) 16, 17
BmiI GGNNCC 5 cut(s) 18, 19, 46, 232, 611
BpiI GAAGAC 1 cut(s) 291
Bpu14I TTCGAA 1 cut(s) 632
BsaJI CCNNGG 2 cut(s) 226, 647
BsaWI WCCGGW 1 cut(s) 440
Bse1I ACTGG 2 cut(s) 93, 482
Bse3DI GCAATG 1 cut(s) 566
BseAI TCCGGA 1 cut(s) 440
BseDI CCNNGG 2 cut(s) 226, 647
BseGI GGATG 1 cut(s) 51
BseMI GCAATG 1 cut(s) 566
BseMII CTCAG 1 cut(s) 633
BseNI ACTGG 2 cut(s) 93, 482
BseSI GKGCMC 2 cut(s) 20, 614
BseXI GCAGC 4 cut(s) 312, 315, 547, 577
BseYI CCCAGC 1 cut(s) 526
BshFI GGCC 3 cut(s) 18, 181, 677
BshNI GGYRCC 1 cut(s) 609
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 1 cut(s) 441
BslFI GGGAC 1 cut(s) 671
BsmFI GGGAC 1 cut(s) 671
BsnI GGCC 3 cut(s) 18, 181, 677
BsoBI CYCGRG 1 cut(s) 173
Bsp119I TTCGAA 1 cut(s) 632
Bsp120I GGGCCC 1 cut(s) 16
Bsp1286I GDGCHC 2 cut(s) 20, 614
Bsp13I TCCGGA 1 cut(s) 440
Bsp1407I TGTACA 1 cut(s) 728
Bsp143I GATC 5 cut(s) 44, 147, 371, 384, 420
BspACI CCGC 3 cut(s) 156, 572, 615
BspANI GGCC 3 cut(s) 18, 181, 677
BspCNI CTCAG 1 cut(s) 632
BspEI TCCGGA 1 cut(s) 440
BspHI TCATGA 2 cut(s) 280, 654
BspLI GGNNCC 5 cut(s) 18, 19, 46, 232, 611
BspPI GGATC 3 cut(s) 39, 52, 379
BspT104I TTCGAA 1 cut(s) 632
BspT107I GGYRCC 1 cut(s) 609
BsrBI CCGCTC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 566
BsrGI TGTACA 1 cut(s) 728
BsrI ACTGG 2 cut(s) 93, 482
BssECI CCNNGG 2 cut(s) 226, 647
BssMI GATC 5 cut(s) 44, 147, 371, 384, 420
BssT1I CCWWGG 1 cut(s) 226
Bst4CI ACNGT 1 cut(s) 328
Bst6I CTCTTC 1 cut(s) 505
BstAPI GCANNNNNTGC 1 cut(s) 580
BstAUI TGTACA 1 cut(s) 728
BstBI TTCGAA 1 cut(s) 632
BstC8I GCNNGC 2 cut(s) 61, 644
BstDEI CTNAG 1 cut(s) 619
BstF5I GGATG 1 cut(s) 51
BstKTI GATC 5 cut(s) 47, 150, 374, 387, 423
BstMBI GATC 5 cut(s) 44, 147, 371, 384, 420
BstMWI GCNNNNNNNGC 2 cut(s) 178, 580
BstNSI RCATGY 3 cut(s) 63, 587, 646
BstSLI GKGCMC 2 cut(s) 20, 614
BstV1I GCAGC 4 cut(s) 312, 315, 547, 577
BstV2I GAAGAC 1 cut(s) 291
BstX2I RGATCY 2 cut(s) 44, 420
BstYI RGATCY 2 cut(s) 44, 420
BsuRI GGCC 3 cut(s) 18, 181, 677
BtsCI GGATG 1 cut(s) 51
BtsI GCAGTG 1 cut(s) 555
BtsIMutI CAGTG 2 cut(s) 475, 555
Cac8I GCNNGC 2 cut(s) 61, 644
CciI TCATGA 2 cut(s) 280, 654
Cfr13I GGNCC 2 cut(s) 16, 17
Csp6I GTAC 3 cut(s) 493, 670, 729
CviAII CATG 7 cut(s) 60, 73, 281, 575, 584, 643, 655
CviJI RGCY 8 cut(s) 18, 165, 172, 181, 222, 306, 677, 714
CviKI_1 RGCY 8 cut(s) 18, 165, 172, 181, 222, 306, 677, 714
CviQI GTAC 3 cut(s) 493, 670, 729
DdeI CTNAG 1 cut(s) 619
DpnI GATC 5 cut(s) 46, 149, 373, 386, 422
DpnII GATC 5 cut(s) 44, 147, 371, 384, 420
Eam1104I CTCTTC 1 cut(s) 505
EarI CTCTTC 1 cut(s) 505
Eco130I CCWWGG 1 cut(s) 226
Eco24I GRGCYC 1 cut(s) 20
Eco88I CYCGRG 1 cut(s) 173
EcoO109I RGGNCCY 1 cut(s) 17
EcoT14I CCWWGG 1 cut(s) 226
EcoT22I ATGCAT 1 cut(s) 585
EcoT38I GRGCYC 1 cut(s) 20
ErhI CCWWGG 1 cut(s) 226
FaeI CATG 7 cut(s) 63, 76, 284, 578, 587, 646, 658
FaqI GGGAC 1 cut(s) 671
FatI CATG 7 cut(s) 59, 72, 280, 574, 583, 642, 654
FauI CCCGC 1 cut(s) 622
FauNDI CATATG 1 cut(s) 40
Fnu4HI GCNGC 5 cut(s) 301, 304, 561, 572, 591
FokI GGATG 1 cut(s) 38
FriOI GRGCYC 1 cut(s) 20
Fsp4HI GCNGC 5 cut(s) 301, 304, 561, 572, 591
GluI GCNGC 5 cut(s) 301, 304, 561, 572, 591
GsaI CCCAGC 1 cut(s) 530
HaeIII GGCC 3 cut(s) 18, 181, 677
HapII CCGG 1 cut(s) 441
Hin1II CATG 7 cut(s) 63, 76, 284, 578, 587, 646, 658
HpaII CCGG 1 cut(s) 441
Hpy188I TCNGA 5 cut(s) 162, 250, 341, 376, 622
Hpy188III TCNNGA 7 cut(s) 175, 186, 281, 332, 441, 655, 718
HpyAV CCTTC 2 cut(s) 202, 639
HpyCH4III ACNGT 1 cut(s) 328
HpyCH4IV ACGT 2 cut(s) 242, 405
HpyCH4V TGCA 4 cut(s) 300, 583, 593, 642
HpyF10VI GCNNNNNNNGC 2 cut(s) 178, 580
HpyF3I CTNAG 1 cut(s) 619
HpySE526I ACGT 2 cut(s) 242, 405
Hsp92II CATG 7 cut(s) 63, 76, 284, 578, 587, 646, 658
Kpn2I TCCGGA 1 cut(s) 440
Kzo9I GATC 5 cut(s) 44, 147, 371, 384, 420
LmnI GCTCC 1 cut(s) 163
LpnPI CCDG 7 cut(s) 77, 106, 326, 454, 463, 512, 683
Lsp1109I GCAGC 4 cut(s) 312, 315, 547, 577
MaeII ACGT 2 cut(s) 242, 405
MaeIII GTNAC 3 cut(s) 84, 238, 449
MalI GATC 5 cut(s) 46, 149, 373, 386, 422
MbiI CCGCTC 1 cut(s) 158
MboI GATC 5 cut(s) 44, 147, 371, 384, 420
MfeI CAATTG 1 cut(s) 604
MflI RGATCY 2 cut(s) 44, 420
MhlI GDGCHC 2 cut(s) 20, 614
MluCI AATT 1 cut(s) 604
MmeI TCCRAC 2 cut(s) 72, 319
MnlI CCTC 7 cut(s) 171, 252, 437, 536, 628, 642, 740
Mph1103I ATGCAT 1 cut(s) 585
MroI TCCGGA 1 cut(s) 440
MspI CCGG 1 cut(s) 441
MunI CAATTG 1 cut(s) 604
MwoI GCNNNNNNNGC 2 cut(s) 178, 580
NdeI CATATG 1 cut(s) 40
NdeII GATC 5 cut(s) 44, 147, 371, 384, 420
NlaIII CATG 7 cut(s) 63, 76, 284, 578, 587, 646, 658
NlaIV GGNNCC 5 cut(s) 18, 19, 46, 232, 611
NmeAIII GCCGAG 4 cut(s) 198, 672, 691, 703
NmuCI GTSAC 2 cut(s) 238, 449
NsiI ATGCAT 1 cut(s) 585
NspI RCATGY 3 cut(s) 63, 587, 646
NspV TTCGAA 1 cut(s) 632
PaeI GCATGC 2 cut(s) 63, 646
PagI TCATGA 2 cut(s) 280, 654
PkrI GCNGC 5 cut(s) 302, 305, 562, 573, 592
Psp1406I AACGTT 1 cut(s) 405
PspFI CCCAGC 1 cut(s) 526
PspN4I GGNNCC 5 cut(s) 18, 19, 46, 232, 611
PspOMI GGGCCC 1 cut(s) 16
PspPI GGNCC 2 cut(s) 16, 17
PsuI RGATCY 2 cut(s) 44, 420
RsaI GTAC 3 cut(s) 494, 671, 730
RsaNI GTAC 3 cut(s) 493, 670, 729
SatI GCNGC 5 cut(s) 301, 304, 561, 572, 591
Sau3AI GATC 5 cut(s) 44, 147, 371, 384, 420
Sau96I GGNCC 2 cut(s) 16, 17
SduI GDGCHC 2 cut(s) 20, 614
SetI ASST 8 cut(s) 167, 174, 245, 408, 429, 631, 639, 653
SfuI TTCGAA 1 cut(s) 632
SphI GCATGC 2 cut(s) 63, 646
Sse9I AATT 1 cut(s) 604
SsiI CCGC 3 cut(s) 156, 572, 615
StyI CCWWGG 1 cut(s) 226
TaaI ACNGT 1 cut(s) 328
TaiI ACGT 2 cut(s) 245, 408
TaqI TCGA 4 cut(s) 146, 331, 489, 632
TasI AATT 1 cut(s) 604
TatI WGTACW 2 cut(s) 669, 728
TauI GCSGC 1 cut(s) 574
TscAI CASTG 2 cut(s) 482, 562
TseFI GTSAC 2 cut(s) 238, 449
TseI GCWGC 4 cut(s) 300, 303, 560, 590
Tsp45I GTSAC 2 cut(s) 238, 449
TspDTI ATGAA 7 cut(s) 61, 269, 297, 306, 408, 643, 671
TspGWI ACGGA 2 cut(s) 379, 384
TspRI CASTG 2 cut(s) 482, 562
XceI RCATGY 3 cut(s) 63, 587, 646
Zsp2I ATGCAT 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.